Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76826.t1 | GAU44816.1 | 61.8 | 89 | 25 | 2 | 1 | 80 | 5 | 93 | 9.30E-18 | 99.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76826.t1 | A0A2Z6NMD3 | 61.8 | 89 | 25 | 2 | 1 | 80 | 5 | 93 | 6.7e-18 | 99.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055056 | MS.gene76826 | 0.800659 | 1.30E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCTATGTTGGTGGTAAAA+TGG | 0.234195 | 1.1:-76645409 | MS.gene76826:CDS |
| AAGGCGAAGGTGGTGGAGAA+AGG | 0.417691 | 1.1:-76646026 | MS.gene76826:CDS |
| ATATCATGGTGGGTTGTTCA+TGG | 0.434787 | 1.1:-76645445 | MS.gene76826:CDS |
| CAAAACTGATTGAAAGTCTA+GGG | 0.463478 | 1.1:-76645335 | MS.gene76826:CDS |
| TCAAAACTGATTGAAAGTCT+AGG | 0.466346 | 1.1:-76645336 | MS.gene76826:CDS |
| TGGAAGAAATGAATTGCGGT+TGG | 0.484666 | 1.1:-76645389 | MS.gene76826:CDS |
| GAGATGCATCTCTGTATTCA+AGG | 0.488933 | 1.1:-76645992 | MS.gene76826:intron |
| AGGTGGTGGAGAAAGGAGAA+GGG | 0.490083 | 1.1:-76646019 | MS.gene76826:CDS |
| AAGGTGGTGGAGAAAGGAGA+AGG | 0.492351 | 1.1:-76646020 | MS.gene76826:CDS |
| ATCTATGAAGGCGAAGGTGG+TGG | 0.496255 | 1.1:-76646033 | None:intergenic |
| GCTTGTGATCCTCGAGGTTG+AGG | 0.515924 | 1.1:+76645293 | None:intergenic |
| GTCATATTACGTATATATCA+TGG | 0.516960 | 1.1:-76645459 | MS.gene76826:CDS |
| CCACCAACATAGATGTTGCT+AGG | 0.517120 | 1.1:+76645417 | None:intergenic |
| TGTCGATGTCGTGTCCTACA+TGG | 0.525376 | 1.1:-76645364 | MS.gene76826:CDS |
| CCTAGCAACATCTATGTTGG+TGG | 0.553229 | 1.1:-76645417 | MS.gene76826:CDS |
| TTCATCTATGAAGGCGAAGG+TGG | 0.561267 | 1.1:-76646036 | None:intergenic |
| GTGGTGGAGAAAGGAGAAGG+GGG | 0.563580 | 1.1:-76646017 | MS.gene76826:CDS |
| TCACCTAGCAACATCTATGT+TGG | 0.577638 | 1.1:-76645420 | MS.gene76826:CDS |
| GGAAGAAATGAATTGCGGTT+GGG | 0.590491 | 1.1:-76645388 | MS.gene76826:CDS |
| AAATTCATCTATGAAGGCGA+AGG | 0.625676 | 1.1:-76646039 | None:intergenic |
| AATATGACATGCCTTTGCGA+TGG | 0.648827 | 1.1:+76645474 | None:intergenic |
| AAAATGGAAGAAATGAATTG+CGG | 0.653416 | 1.1:-76645393 | MS.gene76826:CDS |
| GGTGGTGGAGAAAGGAGAAG+GGG | 0.655309 | 1.1:-76646018 | MS.gene76826:CDS |
| TATTACGTATATATCATGGT+GGG | 0.655518 | 1.1:-76645455 | MS.gene76826:CDS |
| ATTTGTATAGGCCATCGCAA+AGG | 0.657043 | 1.1:-76645485 | MS.gene76826:intron |
| TCAGAAGCACCTCAACCTCG+AGG | 0.701100 | 1.1:-76645302 | MS.gene76826:CDS |
| ATATTACGTATATATCATGG+TGG | 0.711279 | 1.1:-76645456 | MS.gene76826:CDS |
| ACTTATGCTTGTGATCCTCG+AGG | 0.715140 | 1.1:+76645287 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTCACAATTTTTATTTGTTA+TGG | + | chr1.1:76645406-76645425 | None:intergenic | 15.0% |
| !!! | AATTTTGTATCTCTAGTTTA+GGG | - | chr1.1:76645482-76645501 | MS.gene76826:intron | 20.0% |
| !!! | TAATTTTGTATCTCTAGTTT+AGG | - | chr1.1:76645481-76645500 | MS.gene76826:intron | 20.0% |
| !!! | TGTATGTTCTTTATTTGTAT+AGG | - | chr1.1:76645813-76645832 | MS.gene76826:intron | 20.0% |
| ! | AAAATGGAAGAAATGAATTG+CGG | - | chr1.1:76645917-76645936 | MS.gene76826:intron | 25.0% |
| ! | AATCAAAATAGCTACTGTAA+AGG | + | chr1.1:76645677-76645696 | None:intergenic | 25.0% |
| ! | ATATTACGTATATATCATGG+TGG | - | chr1.1:76645854-76645873 | MS.gene76826:intron | 25.0% |
| ! | ATATTCATAAAACTCCTTCA+CGG | - | chr1.1:76645583-76645602 | MS.gene76826:intron | 25.0% |
| ! | GTCATATTACGTATATATCA+TGG | - | chr1.1:76645851-76645870 | MS.gene76826:intron | 25.0% |
| ! | TATTACGTATATATCATGGT+GGG | - | chr1.1:76645855-76645874 | MS.gene76826:intron | 25.0% |
| !!! | TACAGTAGCTATTTTGATTT+TGG | - | chr1.1:76645678-76645697 | MS.gene76826:intron | 25.0% |
| CAAAACTGATTGAAAGTCTA+GGG | - | chr1.1:76645975-76645994 | MS.gene76826:intron | 30.0% | |
| TCAAAACTGATTGAAAGTCT+AGG | - | chr1.1:76645974-76645993 | MS.gene76826:intron | 30.0% | |
| ! | TTTTCTCTTTTGACCCAATA+TGG | - | chr1.1:76645351-76645370 | MS.gene76826:CDS | 30.0% |
| !!! | CAGTTTTGAAATATCCATGT+AGG | + | chr1.1:76645963-76645982 | None:intergenic | 30.0% |
| !!! | TGTTCTTGCTACTGATTTTA+TGG | - | chr1.1:76645725-76645744 | MS.gene76826:intron | 30.0% |
| ACACCTTAACTACACCATAT+TGG | + | chr1.1:76645368-76645387 | None:intergenic | 35.0% | |
| CACCTTAACTACACCATATT+GGG | + | chr1.1:76645367-76645386 | None:intergenic | 35.0% | |
| TATCACAATAACTACCGTGA+AGG | + | chr1.1:76645600-76645619 | None:intergenic | 35.0% | |
| !! | CATCTATGTTGGTGGTAAAA+TGG | - | chr1.1:76645901-76645920 | MS.gene76826:intron | 35.0% |
| AATATGACATGCCTTTGCGA+TGG | + | chr1.1:76645839-76645858 | None:intergenic | 40.0% | |
| ATTTGTATAGGCCATCGCAA+AGG | - | chr1.1:76645825-76645844 | MS.gene76826:intron | 40.0% | |
| GACCCAATATGGTGTAGTTA+AGG | - | chr1.1:76645362-76645381 | MS.gene76826:CDS | 40.0% | |
| GAGATGCATCTCTGTATTCA+AGG | - | chr1.1:76645318-76645337 | MS.gene76826:CDS | 40.0% | |
| GGAAGAAATGAATTGCGGTT+GGG | - | chr1.1:76645922-76645941 | MS.gene76826:intron | 40.0% | |
| TCACCTAGCAACATCTATGT+TGG | - | chr1.1:76645890-76645909 | MS.gene76826:intron | 40.0% | |
| TGGAAGAAATGAATTGCGGT+TGG | - | chr1.1:76645921-76645940 | MS.gene76826:intron | 40.0% | |
| !! | ATATCATGGTGGGTTGTTCA+TGG | - | chr1.1:76645865-76645884 | MS.gene76826:intron | 40.0% |
| ACTTATGCTTGTGATCCTCG+AGG | + | chr1.1:76646026-76646045 | None:intergenic | 45.0% | |
| CCACCAACATAGATGTTGCT+AGG | + | chr1.1:76645896-76645915 | None:intergenic | 45.0% | |
| CCTAGCAACATCTATGTTGG+TGG | - | chr1.1:76645893-76645912 | MS.gene76826:intron | 45.0% | |
| TGTCGATGTCGTGTCCTACA+TGG | - | chr1.1:76645946-76645965 | MS.gene76826:intron | 50.0% | |
| !! | AAGGTGGTGGAGAAAGGAGA+AGG | - | chr1.1:76645290-76645309 | MS.gene76826:CDS | 50.0% |
| !! | AGGTGGTGGAGAAAGGAGAA+GGG | - | chr1.1:76645291-76645310 | MS.gene76826:CDS | 50.0% |
| GCTTGTGATCCTCGAGGTTG+AGG | + | chr1.1:76646020-76646039 | None:intergenic | 55.0% | |
| ! | GTGGTGGAGAAAGGAGAAGG+GGG | - | chr1.1:76645293-76645312 | MS.gene76826:CDS | 55.0% |
| !! | AAGGCGAAGGTGGTGGAGAA+AGG | - | chr1.1:76645284-76645303 | MS.gene76826:CDS | 55.0% |
| !! | GGTGGTGGAGAAAGGAGAAG+GGG | - | chr1.1:76645292-76645311 | MS.gene76826:CDS | 55.0% |
| !! | TCAGAAGCACCTCAACCTCG+AGG | - | chr1.1:76646008-76646027 | MS.gene76826:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 76645281 | 76646051 | 76645281 | ID=MS.gene76826 |
| chr1.1 | mRNA | 76645281 | 76646051 | 76645281 | ID=MS.gene76826.t1;Parent=MS.gene76826 |
| chr1.1 | exon | 76645993 | 76646051 | 76645993 | ID=MS.gene76826.t1.exon1;Parent=MS.gene76826.t1 |
| chr1.1 | CDS | 76645993 | 76646051 | 76645993 | ID=cds.MS.gene76826.t1;Parent=MS.gene76826.t1 |
| chr1.1 | exon | 76645281 | 76645497 | 76645281 | ID=MS.gene76826.t1.exon2;Parent=MS.gene76826.t1 |
| chr1.1 | CDS | 76645281 | 76645497 | 76645281 | ID=cds.MS.gene76826.t1;Parent=MS.gene76826.t1 |
| Gene Sequence |
| Protein sequence |
