Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77322.t1 | XP_003623274.1 | 86.9 | 130 | 7 | 2 | 1 | 126 | 1 | 124 | 4.40E-50 | 207.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77322.t1 | G7KRF0 | 86.9 | 130 | 7 | 2 | 1 | 126 | 1 | 124 | 3.2e-50 | 207.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053031 | MS.gene77322 | 0.804574 | 2.02E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77322.t1 | MTR_7g068050 | 85.470 | 117 | 7 | 2 | 1 | 113 | 1 | 111 | 6.31e-66 | 196 |
| MS.gene77322.t1 | MTR_7g067950 | 51.961 | 102 | 43 | 3 | 30 | 126 | 27 | 127 | 5.32e-22 | 85.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 37 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCTGAATCGTCAGCAATTC+GGG | 0.244167 | 7.2:-35691356 | MS.gene77322:CDS |
| ACCCGAATTGCTGACGATTC+AGG | 0.254329 | 7.2:+35691355 | None:intergenic |
| TAAGAGGGGATTCTCTTATA+AGG | 0.293421 | 7.2:-35692697 | MS.gene77322:CDS |
| GGAGATCGCGGTTTCTTGAT+TGG | 0.347991 | 7.2:+35692575 | None:intergenic |
| ACCCTGAATCGTCAGCAATT+CGG | 0.349585 | 7.2:-35691357 | MS.gene77322:CDS |
| CCCGAATTGCTGACGATTCA+GGG | 0.374699 | 7.2:+35691356 | None:intergenic |
| ATAACTTGGAAAGCAATTTC+AGG | 0.384541 | 7.2:+35691203 | None:intergenic |
| CGCGGTTTCTTGATTGGAGG+GGG | 0.395599 | 7.2:+35692581 | None:intergenic |
| GGAGAACTCCTTCGCAAACT+TGG | 0.406549 | 7.2:-35691226 | MS.gene77322:CDS |
| GTCAGCAATTCGGGTAAGTC+TGG | 0.408786 | 7.2:-35691347 | MS.gene77322:CDS |
| TAGATATCAGTTTCATAACT+TGG | 0.426133 | 7.2:+35691189 | None:intergenic |
| AAGAGGGGATTCTCTTATAA+GGG | 0.477382 | 7.2:-35692696 | MS.gene77322:CDS |
| ATCTCCGATCTTACAAATCA+AGG | 0.478783 | 7.2:-35692558 | MS.gene77322:CDS |
| TAAGGGAAATAGTTATCCTT+CGG | 0.485583 | 7.2:-35692679 | MS.gene77322:CDS |
| CCGCCCCTGGGTATGGGTGA+AGG | 0.491403 | 7.2:-35692726 | None:intergenic |
| TGGGTGAAGGCGATCATAAG+AGG | 0.509307 | 7.2:-35692713 | MS.gene77322:CDS |
| TCTTCCATCGCGTTGTCTGT+TGG | 0.511505 | 7.2:+35691283 | None:intergenic |
| ATCGCGGTTTCTTGATTGGA+GGG | 0.519025 | 7.2:+35692579 | None:intergenic |
| TGATCCTTGATTTGTAAGAT+CGG | 0.527760 | 7.2:+35692554 | None:intergenic |
| TACAATGAATTGCACAAACA+CGG | 0.528310 | 7.2:-35692627 | MS.gene77322:CDS |
| GGTAAGTCTGGAGGTGAACA+AGG | 0.534232 | 7.2:-35691335 | MS.gene77322:CDS |
| GATCGCGGTTTCTTGATTGG+AGG | 0.552553 | 7.2:+35692578 | None:intergenic |
| GCAGCTGTGTTGGTTTCTCA+AGG | 0.564887 | 7.2:-35691247 | MS.gene77322:CDS |
| TGCACACACCGCAGCTGTGT+TGG | 0.571415 | 7.2:-35691257 | MS.gene77322:CDS |
| GGGTGAAGGCGATCATAAGA+GGG | 0.592975 | 7.2:-35692712 | MS.gene77322:CDS |
| ACAATGAATTGCACAAACAC+GGG | 0.606791 | 7.2:-35692626 | MS.gene77322:CDS |
| ATTTGTAAGATCGGAGATCG+CGG | 0.611829 | 7.2:+35692563 | None:intergenic |
| TCGCGGTTTCTTGATTGGAG+GGG | 0.613086 | 7.2:+35692580 | None:intergenic |
| TATCCTTCGGATGATGAGCA+TGG | 0.617244 | 7.2:-35692666 | MS.gene77322:CDS |
| ATTTCAGGCCAAGTTTGCGA+AGG | 0.621581 | 7.2:+35691218 | None:intergenic |
| ACTCCATGCTCATCATCCGA+AGG | 0.646422 | 7.2:+35692663 | None:intergenic |
| GCGGTTTCTTGATTGGAGGG+GGG | 0.647692 | 7.2:+35692582 | None:intergenic |
| TTGAGAAACCAACACAGCTG+CGG | 0.651919 | 7.2:+35691249 | None:intergenic |
| TGTTCCAACAGACAACGCGA+TGG | 0.657088 | 7.2:-35691287 | MS.gene77322:CDS |
| AGCAATTCGGGTAAGTCTGG+AGG | 0.657891 | 7.2:-35691344 | MS.gene77322:CDS |
| GTAAGTCTGGAGGTGAACAA+GGG | 0.696458 | 7.2:-35691334 | MS.gene77322:CDS |
| GGTGAAGGCGATCATAAGAG+GGG | 0.710139 | 7.2:-35692711 | MS.gene77322:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTAAATTAATAATATTTTA+AGG | + | chr7.2:35691992-35692011 | None:intergenic | 0.0% |
| !!! | TTATTTTTTTTAATAAAAAT+TGG | - | chr7.2:35691919-35691938 | MS.gene77322:intron | 0.0% |
| !! | AAAAATTGATATATTTAGAT+GGG | + | chr7.2:35691826-35691845 | None:intergenic | 10.0% |
| !! | GATAAATTAATAAAGATTAA+AGG | - | chr7.2:35692050-35692069 | MS.gene77322:intron | 10.0% |
| !! | TAAAAATTGATATATTTAGA+TGG | + | chr7.2:35691827-35691846 | None:intergenic | 10.0% |
| !!! | AAAAATAGATAAAATGTTTT+AGG | + | chr7.2:35692328-35692347 | None:intergenic | 10.0% |
| !!! | TAAAAATTTCATTCAAATTT+TGG | + | chr7.2:35692252-35692271 | None:intergenic | 10.0% |
| !! | AAAAGAACAAACATAAAAAT+TGG | - | chr7.2:35692206-35692225 | MS.gene77322:intron | 15.0% |
| !! | AAAGTAAAATAAATAGTATG+AGG | - | chr7.2:35692353-35692372 | MS.gene77322:intron | 15.0% |
| !! | AACTCTATTTATTATTTGTT+GGG | - | chr7.2:35691784-35691803 | MS.gene77322:intron | 15.0% |
| !!! | ACTATAAATTTGTTTCTTTA+AGG | - | chr7.2:35691685-35691704 | MS.gene77322:intron | 15.0% |
| !!! | CTAAATAATTCTTTGTATTT+TGG | - | chr7.2:35691859-35691878 | MS.gene77322:intron | 15.0% |
| !!! | TAATCTTTAATTTGAAGTTA+TGG | - | chr7.2:35692028-35692047 | MS.gene77322:intron | 15.0% |
| !! | AAATTAAAGATGGAAATGAA+TGG | + | chr7.2:35691474-35691493 | None:intergenic | 20.0% |
| !! | ATATCAGAAAATAAGTCATT+TGG | - | chr7.2:35692161-35692180 | MS.gene77322:intron | 20.0% |
| !! | ATTAGTAACCGAAAATTTAT+AGG | + | chr7.2:35691886-35691905 | None:intergenic | 20.0% |
| !! | CAAACATAAAAATTGGTAAA+AGG | - | chr7.2:35692213-35692232 | MS.gene77322:intron | 20.0% |
| !! | CAACTCTATTTATTATTTGT+TGG | - | chr7.2:35691783-35691802 | MS.gene77322:intron | 20.0% |
| !! | TAATCCATTGAAATTAAAGA+TGG | + | chr7.2:35691484-35691503 | None:intergenic | 20.0% |
| !! | TTCATAGATGTCAAAATTAA+AGG | + | chr7.2:35691419-35691438 | None:intergenic | 20.0% |
| !!! | AGCGACAAATATTTTTTTTA+AGG | + | chr7.2:35691546-35691565 | None:intergenic | 20.0% |
| !!! | ATTTCCATCTTTAATTTCAA+TGG | - | chr7.2:35691477-35691496 | MS.gene77322:intron | 20.0% |
| !!! | ATTTTGGTCCTATAAATTTT+CGG | - | chr7.2:35691875-35691894 | MS.gene77322:intron | 20.0% |
| !!! | GCGACAAATATTTTTTTTAA+GGG | + | chr7.2:35691545-35691564 | None:intergenic | 20.0% |
| ! | ATCAATAATAACAAGTGAGT+TGG | - | chr7.2:35691380-35691399 | MS.gene77322:intron | 25.0% |
| !! | ATTATTTGTTGGGTTAACTA+TGG | - | chr7.2:35691794-35691813 | MS.gene77322:intron | 25.0% |
| !!! | TTTTAAGGGATCTATGTTAA+AGG | + | chr7.2:35691531-35691550 | None:intergenic | 25.0% |
| ATAACTTGGAAAGCAATTTC+AGG | + | chr7.2:35692715-35692734 | None:intergenic | 30.0% | |
| CATGCAGAAAGTTGAAAATA+GGG | + | chr7.2:35691617-35691636 | None:intergenic | 30.0% | |
| TAAGGGAAATAGTTATCCTT+CGG | - | chr7.2:35691236-35691255 | MS.gene77322:CDS | 30.0% | |
| TACAATGAATTGCACAAACA+CGG | - | chr7.2:35691288-35691307 | MS.gene77322:CDS | 30.0% | |
| TCATGCAGAAAGTTGAAAAT+AGG | + | chr7.2:35691618-35691637 | None:intergenic | 30.0% | |
| !! | TCTTTAAGGTGTATAAGAGA+GGG | - | chr7.2:35691699-35691718 | MS.gene77322:intron | 30.0% |
| !! | TGATCCTTGATTTGTAAGAT+CGG | + | chr7.2:35691364-35691383 | None:intergenic | 30.0% |
| !! | TTCTTTAAGGTGTATAAGAG+AGG | - | chr7.2:35691698-35691717 | MS.gene77322:intron | 30.0% |
| AAGAGGGGATTCTCTTATAA+GGG | - | chr7.2:35691219-35691238 | MS.gene77322:CDS | 35.0% | |
| ACAATGAATTGCACAAACAC+GGG | - | chr7.2:35691289-35691308 | MS.gene77322:CDS | 35.0% | |
| ATCTCCGATCTTACAAATCA+AGG | - | chr7.2:35691357-35691376 | MS.gene77322:CDS | 35.0% | |
| TAAGAGGGGATTCTCTTATA+AGG | - | chr7.2:35691218-35691237 | MS.gene77322:CDS | 35.0% | |
| TTTAGTCAGTGAGCTTAACT+CGG | + | chr7.2:35691758-35691777 | None:intergenic | 35.0% | |
| TTTCAACTTTCTGCATGAGT+CGG | - | chr7.2:35691620-35691639 | MS.gene77322:intron | 35.0% | |
| ATTTGTAAGATCGGAGATCG+CGG | + | chr7.2:35691355-35691374 | None:intergenic | 40.0% | |
| ACCCTGAATCGTCAGCAATT+CGG | - | chr7.2:35692558-35692577 | MS.gene77322:CDS | 45.0% | |
| ATCGCGGTTTCTTGATTGGA+GGG | + | chr7.2:35691339-35691358 | None:intergenic | 45.0% | |
| ATTTCAGGCCAAGTTTGCGA+AGG | + | chr7.2:35692700-35692719 | None:intergenic | 45.0% | |
| GTAAGTCTGGAGGTGAACAA+GGG | - | chr7.2:35692581-35692600 | MS.gene77322:CDS | 45.0% | |
| TATCCTTCGGATGATGAGCA+TGG | - | chr7.2:35691249-35691268 | MS.gene77322:CDS | 45.0% | |
| TTGAGAAACCAACACAGCTG+CGG | + | chr7.2:35692669-35692688 | None:intergenic | 45.0% | |
| ! | TCGGTTGGTATGCATGAGTT+GGG | + | chr7.2:35691739-35691758 | None:intergenic | 45.0% |
| ACCCGAATTGCTGACGATTC+AGG | + | chr7.2:35692563-35692582 | None:intergenic | 50.0% | |
| ACTCCATGCTCATCATCCGA+AGG | + | chr7.2:35691255-35691274 | None:intergenic | 50.0% | |
| AGCAATTCGGGTAAGTCTGG+AGG | - | chr7.2:35692571-35692590 | MS.gene77322:CDS | 50.0% | |
| CCCGAATTGCTGACGATTCA+GGG | + | chr7.2:35692562-35692581 | None:intergenic | 50.0% | |
| CCCTGAATCGTCAGCAATTC+GGG | - | chr7.2:35692559-35692578 | MS.gene77322:CDS | 50.0% | |
| GATCGCGGTTTCTTGATTGG+AGG | + | chr7.2:35691340-35691359 | None:intergenic | 50.0% | |
| GGAGAACTCCTTCGCAAACT+TGG | - | chr7.2:35692689-35692708 | MS.gene77322:CDS | 50.0% | |
| GGAGATCGCGGTTTCTTGAT+TGG | + | chr7.2:35691343-35691362 | None:intergenic | 50.0% | |
| GGGTGAAGGCGATCATAAGA+GGG | - | chr7.2:35691203-35691222 | MS.gene77322:CDS | 50.0% | |
| GGTAAGTCTGGAGGTGAACA+AGG | - | chr7.2:35692580-35692599 | MS.gene77322:CDS | 50.0% | |
| GGTGAAGGCGATCATAAGAG+GGG | - | chr7.2:35691204-35691223 | MS.gene77322:CDS | 50.0% | |
| GTCAGCAATTCGGGTAAGTC+TGG | - | chr7.2:35692568-35692587 | MS.gene77322:CDS | 50.0% | |
| GTCAGTGAGCTTAACTCGGT+TGG | + | chr7.2:35691754-35691773 | None:intergenic | 50.0% | |
| TCGCGGTTTCTTGATTGGAG+GGG | + | chr7.2:35691338-35691357 | None:intergenic | 50.0% | |
| TCTTCCATCGCGTTGTCTGT+TGG | + | chr7.2:35692635-35692654 | None:intergenic | 50.0% | |
| TGGGTGAAGGCGATCATAAG+AGG | - | chr7.2:35691202-35691221 | MS.gene77322:CDS | 50.0% | |
| TGTTCCAACAGACAACGCGA+TGG | - | chr7.2:35692628-35692647 | MS.gene77322:CDS | 50.0% | |
| TTGATTGGAGGGGGGAGAAT+TGG | + | chr7.2:35691328-35691347 | None:intergenic | 50.0% | |
| ! | CGGTTGGTATGCATGAGTTG+GGG | + | chr7.2:35691738-35691757 | None:intergenic | 50.0% |
| ! | CTCGGTTGGTATGCATGAGT+TGG | + | chr7.2:35691740-35691759 | None:intergenic | 50.0% |
| !! | GCAGCTGTGTTGGTTTCTCA+AGG | - | chr7.2:35692668-35692687 | MS.gene77322:CDS | 50.0% |
| CGCGGTTTCTTGATTGGAGG+GGG | + | chr7.2:35691337-35691356 | None:intergenic | 55.0% | |
| GAGGGGGGAGAATTGGATTC+TGG | + | chr7.2:35691321-35691340 | None:intergenic | 55.0% | |
| GCGGTTTCTTGATTGGAGGG+GGG | + | chr7.2:35691336-35691355 | None:intergenic | 55.0% | |
| TGCACACACCGCAGCTGTGT+TGG | - | chr7.2:35692658-35692677 | MS.gene77322:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 35691201 | 35692736 | 35691201 | ID=MS.gene77322 |
| chr7.2 | mRNA | 35691201 | 35692736 | 35691201 | ID=MS.gene77322.t1;Parent=MS.gene77322 |
| chr7.2 | exon | 35692544 | 35692736 | 35692544 | ID=MS.gene77322.t1.exon1;Parent=MS.gene77322.t1 |
| chr7.2 | CDS | 35692544 | 35692736 | 35692544 | ID=cds.MS.gene77322.t1;Parent=MS.gene77322.t1 |
| chr7.2 | exon | 35691201 | 35691388 | 35691201 | ID=MS.gene77322.t1.exon2;Parent=MS.gene77322.t1 |
| chr7.2 | CDS | 35691201 | 35691388 | 35691201 | ID=cds.MS.gene77322.t1;Parent=MS.gene77322.t1 |
| Gene Sequence |
| Protein sequence |
