Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77580.t1 | XP_003601758.1 | 97.6 | 123 | 3 | 0 | 1 | 123 | 1 | 123 | 3.40E-63 | 250.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77580.t1 | Q9FZK4 | 82.9 | 123 | 20 | 1 | 1 | 123 | 1 | 122 | 3.2e-54 | 212.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77580.t1 | G7JBF4 | 97.6 | 123 | 3 | 0 | 1 | 123 | 1 | 123 | 2.4e-63 | 250.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057321 | MS.gene77580 | 0.813746 | 2.19E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77580.t1 | MTR_3g085070 | 97.561 | 123 | 3 | 0 | 1 | 123 | 1 | 123 | 1.87e-88 | 252 |
| MS.gene77580.t1 | MTR_8g080790 | 90.244 | 123 | 12 | 0 | 1 | 123 | 1 | 123 | 1.43e-81 | 235 |
| MS.gene77580.t1 | MTR_2g461940 | 80.851 | 94 | 12 | 1 | 1 | 94 | 1 | 88 | 3.12e-49 | 153 |
| MS.gene77580.t1 | MTR_7g055610 | 35.246 | 122 | 71 | 5 | 3 | 119 | 19 | 137 | 4.08e-11 | 58.9 |
| MS.gene77580.t1 | MTR_7g057520 | 35.246 | 122 | 71 | 5 | 3 | 119 | 12 | 130 | 4.75e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77580.t1 | AT1G27970 | 82.114 | 123 | 22 | 0 | 1 | 123 | 4 | 126 | 5.08e-74 | 216 |
| MS.gene77580.t1 | AT1G27310 | 82.927 | 123 | 20 | 1 | 1 | 123 | 1 | 122 | 2.47e-73 | 214 |
| MS.gene77580.t1 | AT1G27970 | 81.356 | 118 | 22 | 0 | 1 | 118 | 4 | 121 | 1.17e-69 | 205 |
| MS.gene77580.t1 | AT1G11570 | 59.504 | 121 | 46 | 1 | 6 | 123 | 12 | 132 | 9.05e-47 | 147 |
| MS.gene77580.t1 | AT1G11570 | 59.504 | 121 | 46 | 1 | 6 | 123 | 33 | 153 | 1.19e-46 | 147 |
| MS.gene77580.t1 | AT1G11570 | 57.426 | 101 | 40 | 1 | 6 | 103 | 12 | 112 | 1.52e-34 | 116 |
| MS.gene77580.t1 | AT1G11570 | 57.426 | 101 | 40 | 1 | 6 | 103 | 33 | 133 | 2.49e-34 | 116 |
| MS.gene77580.t1 | AT5G48650 | 37.302 | 126 | 70 | 6 | 1 | 120 | 11 | 133 | 2.64e-12 | 62.4 |
Find 39 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTGAAGGTCAGCAAATTA+TGG | 0.205195 | 3.2:-73145231 | MS.gene77580:CDS |
| TTTGAAGGTCAGCAAATTAT+GGG | 0.362536 | 3.2:-73145230 | MS.gene77580:CDS |
| AATGCCTTTGACAATGCGTT+TGG | 0.385167 | 3.2:+73145332 | None:intergenic |
| GGTTCTATGCTCACCTTTGA+AGG | 0.402799 | 3.2:-73145245 | MS.gene77580:CDS |
| CGTGTTGTTCGCCGGCGAGC+TGG | 0.403242 | 3.2:+73145081 | None:intergenic |
| CGCATTGTCAAAGGCATTCG+TGG | 0.412022 | 3.2:-73145327 | MS.gene77580:CDS |
| CATAATTTGCTGACCTTCAA+AGG | 0.412917 | 3.2:+73145232 | None:intergenic |
| CTTGCGGTGTTGGTATCAAA+TGG | 0.429115 | 3.2:+73143973 | None:intergenic |
| TTGGTAGAGAGTGGCGAGAT+TGG | 0.433392 | 3.2:+73145271 | None:intergenic |
| TGGTAGAGAGTGGCGAGATT+GGG | 0.451654 | 3.2:+73145272 | None:intergenic |
| TTCTTCACGCATAGTTCAAA+CGG | 0.464644 | 3.2:+73143922 | None:intergenic |
| TGATGGAGTGATGGCACTGC+TGG | 0.466864 | 3.2:+73145168 | None:intergenic |
| AATTGACCTGACTGAATTTG+AGG | 0.471209 | 3.2:+73145057 | None:intergenic |
| TTTGAGGGCGTGTTGTTCGC+CGG | 0.484969 | 3.2:+73145073 | None:intergenic |
| ACCGTCGATTGTCAACCTTC+TGG | 0.514517 | 3.2:-73145143 | MS.gene77580:CDS |
| ATTGGGACGGTTGGTGTCGA+AGG | 0.521383 | 3.2:+73145289 | None:intergenic |
| GAGTGATGGCACTGCTGGAA+AGG | 0.524413 | 3.2:+73145173 | None:intergenic |
| TTGACAATCGACGGTGGTGA+TGG | 0.528680 | 3.2:+73145151 | None:intergenic |
| TAATAGCTTCCTTGCGGTGT+TGG | 0.531758 | 3.2:+73143963 | None:intergenic |
| ATTGACCTGACTGAATTTGA+GGG | 0.536270 | 3.2:+73145058 | None:intergenic |
| ATGCCGCCGTTAGCGCCAGA+AGG | 0.549119 | 3.2:+73145128 | None:intergenic |
| TGTCAACCTTCTGGCGCTAA+CGG | 0.557071 | 3.2:-73145134 | MS.gene77580:CDS |
| AGTGGCGAGATTGGGACGGT+TGG | 0.563337 | 3.2:+73145280 | None:intergenic |
| GGGACGGTTGGTGTCGAAGG+TGG | 0.565989 | 3.2:+73145292 | None:intergenic |
| AGGTGAGCATAGAACCATCT+TGG | 0.568405 | 3.2:+73145252 | None:intergenic |
| CTCGCCACTCTCTACCAAGA+TGG | 0.569246 | 3.2:-73145266 | MS.gene77580:CDS |
| ACACGCCCTCAAATTCAGTC+AGG | 0.570302 | 3.2:-73145063 | MS.gene77580:intron |
| GGATCCAAACGCATTGTCAA+AGG | 0.574827 | 3.2:-73145336 | MS.gene77580:CDS |
| AGTGGTAATCTCCAGCTCGC+CGG | 0.581878 | 3.2:-73145092 | MS.gene77580:CDS |
| AGTGATGGCACTGCTGGAAA+GGG | 0.591260 | 3.2:+73145174 | None:intergenic |
| GGCATGCTTGTCTTTGTCAG+TGG | 0.596448 | 3.2:-73145110 | MS.gene77580:CDS |
| CATTTGATACCAACACCGCA+AGG | 0.621557 | 3.2:-73143972 | MS.gene77580:CDS |
| GCCAGAAGGTTGACAATCGA+CGG | 0.623192 | 3.2:+73145142 | None:intergenic |
| AGAACCATCTTGGTAGAGAG+TGG | 0.624728 | 3.2:+73145262 | None:intergenic |
| CGACGGTGGTGATGGAGTGA+TGG | 0.635677 | 3.2:+73145159 | None:intergenic |
| AACACATAATAGCTTCCTTG+CGG | 0.638641 | 3.2:+73143957 | None:intergenic |
| CAACCTTCTGGCGCTAACGG+CGG | 0.643186 | 3.2:-73145131 | MS.gene77580:CDS |
| AGAGAGTGGCGAGATTGGGA+CGG | 0.652017 | 3.2:+73145276 | None:intergenic |
| AGAAGGTTGACAATCGACGG+TGG | 0.721439 | 3.2:+73145145 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTCTATTTGTTAATTTTA+GGG | - | chr3.2:73144248-73144267 | MS.gene77580:intron | 10.0% |
| !! | AAATCCATCATAATAATAAT+AGG | + | chr3.2:73144907-73144926 | None:intergenic | 15.0% |
| !! | AGAATTGTAAAATGTTAATT+AGG | - | chr3.2:73144571-73144590 | MS.gene77580:intron | 15.0% |
| !! | TGATATAATCAACAATTTAA+GGG | - | chr3.2:73144864-73144883 | MS.gene77580:intron | 15.0% |
| !! | TTGATATAATCAACAATTTA+AGG | - | chr3.2:73144863-73144882 | MS.gene77580:intron | 15.0% |
| !!! | ATTAACATTTTACAATTCTA+AGG | + | chr3.2:73144570-73144589 | None:intergenic | 15.0% |
| !!! | GTATTATCAATATTCTAAAA+TGG | - | chr3.2:73144543-73144562 | MS.gene77580:intron | 15.0% |
| !!! | GTTTTCTATTTGTTAATTTT+AGG | - | chr3.2:73144247-73144266 | MS.gene77580:intron | 15.0% |
| !!! | TATTATTATTATGATGGATT+TGG | - | chr3.2:73144906-73144925 | MS.gene77580:intron | 15.0% |
| !!! | TTTCATGATTAAGTTTTAAT+TGG | - | chr3.2:73144322-73144341 | MS.gene77580:intron | 15.0% |
| !!! | TTTTATTTTAGTGATCATTT+TGG | - | chr3.2:73144814-73144833 | MS.gene77580:intron | 15.0% |
| !! | ATAATACCAATCAAATTTCA+GGG | + | chr3.2:73144530-73144549 | None:intergenic | 20.0% |
| !! | CATGAAAAACTTAAATAACA+CGG | + | chr3.2:73144309-73144328 | None:intergenic | 20.0% |
| !! | TTTGCCTATTATTATTATGA+TGG | - | chr3.2:73144900-73144919 | MS.gene77580:intron | 20.0% |
| !!! | TAGAGTGAAATTTCTAATTT+TGG | - | chr3.2:73145027-73145046 | MS.gene77580:intron | 20.0% |
| !!! | TGGAATATTGTTATTGTAAA+TGG | - | chr3.2:73144834-73144853 | MS.gene77580:intron | 20.0% |
| ! | AAAACACCATTATCAATATG+AGG | + | chr3.2:73145059-73145078 | None:intergenic | 25.0% |
| ! | AACAATTTAAGGGACTAAAT+TGG | - | chr3.2:73144874-73144893 | MS.gene77580:intron | 25.0% |
| ! | ATTTCACTCTAATCATGAAT+TGG | + | chr3.2:73145018-73145037 | None:intergenic | 25.0% |
| ! | CAAATTTCAGTAACATATGA+AGG | - | chr3.2:73145099-73145118 | MS.gene77580:CDS | 25.0% |
| ! | CTTCATATGTTACTGAAATT+TGG | + | chr3.2:73145101-73145120 | None:intergenic | 25.0% |
| ! | GATAATACCAATCAAATTTC+AGG | + | chr3.2:73144531-73144550 | None:intergenic | 25.0% |
| ! | GTTAAATCATCAATATCACT+TGG | - | chr3.2:73145173-73145192 | MS.gene77580:CDS | 25.0% |
| ! | TCTATCTGGACAATAATAAA+TGG | + | chr3.2:73145144-73145163 | None:intergenic | 25.0% |
| ! | TTGGAATGTCAAAATTACAT+AGG | + | chr3.2:73144697-73144716 | None:intergenic | 25.0% |
| !! | AATTAGGTTTTTCTATCTGT+TGG | - | chr3.2:73144587-73144606 | MS.gene77580:intron | 25.0% |
| !! | AGTTTTCCTCATATTGATAA+TGG | - | chr3.2:73145050-73145069 | MS.gene77580:intron | 25.0% |
| !!! | CATGATTAAGTTTTAATTGG+TGG | - | chr3.2:73144325-73144344 | MS.gene77580:intron | 25.0% |
| AGTTAGATTCATCTCTTTGT+TGG | + | chr3.2:73144787-73144806 | None:intergenic | 30.0% | |
| ATTTCAGTAACATATGAAGG+CGG | - | chr3.2:73145102-73145121 | MS.gene77580:CDS | 30.0% | |
| TCAAATTTCAGGGATCAAAT+TGG | + | chr3.2:73144520-73144539 | None:intergenic | 30.0% | |
| TGTCATGTTAGAAATGCATT+AGG | + | chr3.2:73144734-73144753 | None:intergenic | 30.0% | |
| TTAACAATCACAGTTCTATC+TGG | + | chr3.2:73145158-73145177 | None:intergenic | 30.0% | |
| TTCTAACATGACAAACGTAT+CGG | - | chr3.2:73144741-73144760 | MS.gene77580:intron | 30.0% | |
| TTTGAAGGTCAGCAAATTAT+GGG | - | chr3.2:73144034-73144053 | MS.gene77580:intron | 30.0% | |
| TTTGATCCCTGAAATTTGAT+TGG | - | chr3.2:73144521-73144540 | MS.gene77580:intron | 30.0% | |
| ! | TGAAATTTGGTCTTTCTCTA+TGG | + | chr3.2:73145088-73145107 | None:intergenic | 30.0% |
| ! | TTGCAACTTTATCTGGTTTA+GGG | - | chr3.2:73144407-73144426 | MS.gene77580:intron | 30.0% |
| !!! | CTTGTTTTTGTGATTGTAGT+TGG | - | chr3.2:73144437-73144456 | MS.gene77580:intron | 30.0% |
| AACACATAATAGCTTCCTTG+CGG | + | chr3.2:73145310-73145329 | None:intergenic | 35.0% | |
| AATTGACCTGACTGAATTTG+AGG | + | chr3.2:73144210-73144229 | None:intergenic | 35.0% | |
| ATTGACCTGACTGAATTTGA+GGG | + | chr3.2:73144209-73144228 | None:intergenic | 35.0% | |
| CAAGATTGAGAAATAAGAGC+AGG | + | chr3.2:73145233-73145252 | None:intergenic | 35.0% | |
| CTGTCGTAGGATTTCAATAT+TGG | - | chr3.2:73144485-73144504 | MS.gene77580:intron | 35.0% | |
| TAAATTCTCGTTCTGTGGTT+GGG | - | chr3.2:73144968-73144987 | MS.gene77580:intron | 35.0% | |
| TCACTCTAATCATGAATTGG+TGG | + | chr3.2:73145015-73145034 | None:intergenic | 35.0% | |
| TCATTGTTCATTTGCATGCT+TGG | - | chr3.2:73144639-73144658 | MS.gene77580:intron | 35.0% | |
| TGAACTAAATTCTCGTTCTG+TGG | - | chr3.2:73144963-73144982 | MS.gene77580:intron | 35.0% | |
| TGAGAAATAAGAGCAGGATT+AGG | + | chr3.2:73145227-73145246 | None:intergenic | 35.0% | |
| TTAGGAGCTAAGTGTGTATT+TGG | + | chr3.2:73144716-73144735 | None:intergenic | 35.0% | |
| ! | CTTTGAAGGTCAGCAAATTA+TGG | - | chr3.2:73144033-73144052 | MS.gene77580:intron | 35.0% |
| ! | GTTGCAACTTTATCTGGTTT+AGG | - | chr3.2:73144406-73144425 | MS.gene77580:intron | 35.0% |
| !! | CATAATTTGCTGACCTTCAA+AGG | + | chr3.2:73144035-73144054 | None:intergenic | 35.0% |
| !! | GTTAATAGTTTTGCTGTCGT+AGG | - | chr3.2:73144472-73144491 | MS.gene77580:intron | 35.0% |
| AATGCCTTTGACAATGCGTT+TGG | + | chr3.2:73143935-73143954 | None:intergenic | 40.0% | |
| CATTTGCATGCTTGGATTTG+CGG | - | chr3.2:73144647-73144666 | MS.gene77580:intron | 40.0% | |
| CTAAATTCTCGTTCTGTGGT+TGG | - | chr3.2:73144967-73144986 | MS.gene77580:intron | 40.0% | |
| TCTAGCGTTGCAACTTTATC+TGG | - | chr3.2:73144400-73144419 | MS.gene77580:intron | 40.0% | |
| AGAAAACCCTAGAGAGAGAG+AGG | + | chr3.2:73144234-73144253 | None:intergenic | 45.0% | |
| AGAACCATCTTGGTAGAGAG+TGG | + | chr3.2:73144005-73144024 | None:intergenic | 45.0% | |
| AGGTGAGCATAGAACCATCT+TGG | + | chr3.2:73144015-73144034 | None:intergenic | 45.0% | |
| CAATTCCCTCTCTCTCTCTA+GGG | - | chr3.2:73144225-73144244 | MS.gene77580:intron | 45.0% | |
| CATTTGATACCAACACCGCA+AGG | - | chr3.2:73145292-73145311 | MS.gene77580:CDS | 45.0% | |
| GAAAACCCTAGAGAGAGAGA+GGG | + | chr3.2:73144233-73144252 | None:intergenic | 45.0% | |
| GGATCCAAACGCATTGTCAA+AGG | - | chr3.2:73143928-73143947 | MS.gene77580:CDS | 45.0% | |
| GGTTCTATGCTCACCTTTGA+AGG | - | chr3.2:73144019-73144038 | MS.gene77580:intron | 45.0% | |
| TAATAGCTTCCTTGCGGTGT+TGG | + | chr3.2:73145304-73145323 | None:intergenic | 45.0% | |
| TCAATTCCCTCTCTCTCTCT+AGG | - | chr3.2:73144224-73144243 | MS.gene77580:intron | 45.0% | |
| TGGTTGGGCAATGGGAATTA+GGG | - | chr3.2:73144983-73145002 | MS.gene77580:intron | 45.0% | |
| !! | CTTGCGGTGTTGGTATCAAA+TGG | + | chr3.2:73145294-73145313 | None:intergenic | 45.0% |
| ACACGCCCTCAAATTCAGTC+AGG | - | chr3.2:73144201-73144220 | MS.gene77580:intron | 50.0% | |
| AGAAGGTTGACAATCGACGG+TGG | + | chr3.2:73144122-73144141 | None:intergenic | 50.0% | |
| AGTGATGGCACTGCTGGAAA+GGG | + | chr3.2:73144093-73144112 | None:intergenic | 50.0% | |
| GCCAGAAGGTTGACAATCGA+CGG | + | chr3.2:73144125-73144144 | None:intergenic | 50.0% | |
| GCTTGGATTTGCGGTGAGTT+TGG | - | chr3.2:73144656-73144675 | MS.gene77580:intron | 50.0% | |
| GGCATGCTTGTCTTTGTCAG+TGG | - | chr3.2:73144154-73144173 | MS.gene77580:intron | 50.0% | |
| GTGGTTGGGCAATGGGAATT+AGG | - | chr3.2:73144982-73145001 | MS.gene77580:intron | 50.0% | |
| TCGTTCTGTGGTTGGGCAAT+GGG | - | chr3.2:73144975-73144994 | MS.gene77580:intron | 50.0% | |
| TGGTAGAGAGTGGCGAGATT+GGG | + | chr3.2:73143995-73144014 | None:intergenic | 50.0% | |
| TGTCAACCTTCTGGCGCTAA+CGG | - | chr3.2:73144130-73144149 | MS.gene77580:intron | 50.0% | |
| TTGACAATCGACGGTGGTGA+TGG | + | chr3.2:73144116-73144135 | None:intergenic | 50.0% | |
| TTGGTAGAGAGTGGCGAGAT+TGG | + | chr3.2:73143996-73144015 | None:intergenic | 50.0% | |
| ! | ACCGTCGATTGTCAACCTTC+TGG | - | chr3.2:73144121-73144140 | MS.gene77580:intron | 50.0% |
| !! | CGCATTGTCAAAGGCATTCG+TGG | - | chr3.2:73143937-73143956 | MS.gene77580:CDS | 50.0% |
| AGAGAGTGGCGAGATTGGGA+CGG | + | chr3.2:73143991-73144010 | None:intergenic | 55.0% | |
| AGTGGTAATCTCCAGCTCGC+CGG | - | chr3.2:73144172-73144191 | MS.gene77580:intron | 55.0% | |
| CTCGCCACTCTCTACCAAGA+TGG | - | chr3.2:73143998-73144017 | MS.gene77580:intron | 55.0% | |
| CTCGTTCTGTGGTTGGGCAA+TGG | - | chr3.2:73144974-73144993 | MS.gene77580:intron | 55.0% | |
| GAGTGATGGCACTGCTGGAA+AGG | + | chr3.2:73144094-73144113 | None:intergenic | 55.0% | |
| TGATGGAGTGATGGCACTGC+TGG | + | chr3.2:73144099-73144118 | None:intergenic | 55.0% | |
| ! | TTTGAGGGCGTGTTGTTCGC+CGG | + | chr3.2:73144194-73144213 | None:intergenic | 55.0% |
| !! | ATTGGGACGGTTGGTGTCGA+AGG | + | chr3.2:73143978-73143997 | None:intergenic | 55.0% |
| AGTGGCGAGATTGGGACGGT+TGG | + | chr3.2:73143987-73144006 | None:intergenic | 60.0% | |
| CAACCTTCTGGCGCTAACGG+CGG | - | chr3.2:73144133-73144152 | MS.gene77580:intron | 60.0% | |
| !! | CGACGGTGGTGATGGAGTGA+TGG | + | chr3.2:73144108-73144127 | None:intergenic | 60.0% |
| ATGCCGCCGTTAGCGCCAGA+AGG | + | chr3.2:73144139-73144158 | None:intergenic | 65.0% | |
| !! | GGGACGGTTGGTGTCGAAGG+TGG | + | chr3.2:73143975-73143994 | None:intergenic | 65.0% |
| ! | CGTGTTGTTCGCCGGCGAGC+TGG | + | chr3.2:73144186-73144205 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 73143926 | 73145360 | 73143926 | ID=MS.gene77580 |
| chr3.2 | mRNA | 73143926 | 73145360 | 73143926 | ID=MS.gene77580.t1;Parent=MS.gene77580 |
| chr3.2 | exon | 73145064 | 73145360 | 73145064 | ID=MS.gene77580.t1.exon1;Parent=MS.gene77580.t1 |
| chr3.2 | CDS | 73145064 | 73145360 | 73145064 | ID=cds.MS.gene77580.t1;Parent=MS.gene77580.t1 |
| chr3.2 | exon | 73143926 | 73144000 | 73143926 | ID=MS.gene77580.t1.exon2;Parent=MS.gene77580.t1 |
| chr3.2 | CDS | 73143926 | 73144000 | 73143926 | ID=cds.MS.gene77580.t1;Parent=MS.gene77580.t1 |
| Gene Sequence |
| Protein sequence |
