AlfalfaGEDB | MS.gene77939

M. sativa cultivar XinJiangDaYe / MS.gene77939

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00319.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77939.t1	XP_013442640.1	81.7	235	43	0	1	235	1	235	2.10E-103	385.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77939.t1	Q9FKK2	49.7	167	81	2	5	168	3	169	4.2e-36	153.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77939.t1	A0A072TSY1	81.7	235	43	0	1	235	1	235	1.5e-103	385.6

TFs/TRs:

Gene ID	Type	Classification
MS.gene77939.t1	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene77939	MS.gene068836	PPI
MS.gene77939	MS.gene22094	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77939.t1	MTR_0121s0080	81.702	235	43	0	1	235	1	235	4.37e-139	392
MS.gene77939.t1	MTR_7g062350	78.414	227	48	1	10	235	21	247	3.38e-126	360
MS.gene77939.t1	MTR_8g043650	72.103	233	64	1	1	232	1	233	8.63e-121	346
MS.gene77939.t1	MTR_2g030740	70.320	219	65	0	1	219	1	219	3.70e-111	321
MS.gene77939.t1	MTR_1g105920	70.776	219	64	0	1	219	1	219	1.27e-110	320
MS.gene77939.t1	MTR_1g105910	68.444	225	71	0	1	225	1	225	9.35e-109	315
MS.gene77939.t1	MTR_1g012570	69.369	222	68	0	1	222	1	222	8.23e-108	313
MS.gene77939.t1	MTR_2g093190	67.660	235	58	3	1	225	1	227	2.67e-104	303
MS.gene77939.t1	MTR_1g108510	65.532	235	63	3	1	225	1	227	3.40e-103	301
MS.gene77939.t1	MTR_3g052920	66.667	219	73	0	1	219	1	219	9.80e-103	300
MS.gene77939.t1	MTR_3g030770	65.532	235	63	3	1	225	1	227	6.09e-102	298
MS.gene77939.t1	MTR_1g105905	66.667	216	71	1	18	232	1	216	1.14e-100	294
MS.gene77939.t1	MTR_1g108580	64.407	236	64	3	1	225	1	227	3.25e-99	291
MS.gene77939.t1	MTR_2g085280	60.169	236	93	1	1	235	1	236	4.19e-99	291
MS.gene77939.t1	MTR_2g085250	60.169	236	93	1	1	235	1	236	1.83e-98	289
MS.gene77939.t1	MTR_1g075600	61.778	225	86	0	1	225	1	225	1.93e-96	284
MS.gene77939.t1	MTR_5g053390	61.333	225	87	0	1	225	1	225	2.07e-93	276
MS.gene77939.t1	MTR_1g077360	59.556	225	91	0	1	225	1	225	2.80e-93	276
MS.gene77939.t1	MTR_5g055100	58.051	236	98	1	1	235	1	236	3.72e-93	276
MS.gene77939.t1	MTR_5g045560	58.667	225	93	0	1	225	1	225	1.12e-88	264
MS.gene77939.t1	MTR_3g052870	61.644	219	65	2	1	219	1	200	6.50e-87	259
MS.gene77939.t1	MTR_1g106070	69.061	181	56	0	1	181	1	181	1.07e-86	257
MS.gene77939.t1	MTR_3g030780	83.333	132	22	0	1	132	1	132	5.16e-74	223
MS.gene77939.t1	MTR_0121s0100	71.212	132	38	0	1	132	1	132	2.87e-59	186
MS.gene77939.t1	MTR_1g075570	52.247	178	71	1	18	195	1	164	1.05e-48	160
MS.gene77939.t1	MTR_3g080940	48.684	152	78	0	9	160	9	160	3.29e-47	157
MS.gene77939.t1	MTR_1g108500	70.103	97	8	1	22	118	110	185	7.57e-36	131
MS.gene77939.t1	MTR_3g067910	54.902	102	46	0	21	122	2	103	1.03e-31	114
MS.gene77939.t1	MTR_8g086290	35.593	177	104	2	5	178	8	177	2.17e-29	110
MS.gene77939.t1	MTR_2g105290	35.542	166	97	3	5	167	8	166	4.01e-28	107
MS.gene77939.t1	MTR_3g067875	65.672	67	23	0	18	84	1	67	1.35e-23	91.7
MS.gene77939.t1	MTR_7g028448	40.580	138	70	3	22	159	25	150	2.12e-23	94.4
MS.gene77939.t1	MTR_4g036915	40.580	138	70	3	22	159	25	150	2.12e-23	94.4
MS.gene77939.t1	MTR_4g127140	33.526	173	101	3	5	167	8	176	2.89e-20	86.7
MS.gene77939.t1	MTR_3g067870	59.677	62	25	0	15	76	82	143	6.09e-19	82.0
MS.gene77939.t1	MTR_1g054265	51.282	78	38	0	9	86	2	79	6.79e-19	82.4
MS.gene77939.t1	MTR_1g047550	53.623	69	32	0	9	77	2	70	6.88e-19	82.8
MS.gene77939.t1	MTR_3g067915	58.209	67	28	0	60	126	30	96	1.32e-18	79.3
MS.gene77939.t1	MTR_8g079502	53.623	69	32	0	9	77	2	70	1.40e-18	82.0
MS.gene77939.t1	MTR_4g131030	37.324	142	64	4	9	125	2	143	8.87e-18	80.5
MS.gene77939.t1	MTR_1g063160	47.436	78	41	0	9	86	2	79	2.13e-17	78.6
MS.gene77939.t1	MTR_8g051580	50.725	69	34	0	9	77	2	70	6.08e-17	77.4
MS.gene77939.t1	MTR_8g046350	50.725	69	34	0	9	77	2	70	3.17e-16	75.1
MS.gene77939.t1	MTR_4g094632	37.273	110	67	1	9	118	2	109	6.64e-16	73.9
MS.gene77939.t1	MTR_8g022970	32.278	158	94	3	8	159	17	167	6.43e-14	69.3
MS.gene77939.t1	MTR_4g094638	47.887	71	37	0	9	79	2	72	7.07e-14	68.9
MS.gene77939.t1	MTR_3g005530	44.118	68	38	0	9	76	2	69	9.93e-14	69.3
MS.gene77939.t1	MTR_4g093970	35.714	112	61	2	10	110	29	140	1.06e-13	69.7
MS.gene77939.t1	MTR_4g084740	36.842	95	59	1	9	102	2	96	4.91e-13	68.9
MS.gene77939.t1	MTR_3g452380	36.364	110	52	1	3	112	11	102	6.79e-13	67.4
MS.gene77939.t1	MTR_5g066180	44.118	68	38	0	9	76	2	69	7.49e-13	67.0
MS.gene77939.t1	MTR_3g102570	40.196	102	58	3	9	109	2	101	7.75e-13	68.2
MS.gene77939.t1	MTR_4g102530	36.842	95	60	0	10	104	3	97	1.47e-12	65.9
MS.gene77939.t1	MTR_5g031000	29.577	213	110	8	9	186	2	209	2.44e-12	65.9
MS.gene77939.t1	MTR_8g033270	42.647	68	39	0	9	76	2	69	2.98e-12	65.9
MS.gene77939.t1	MTR_8g087860	30.818	159	101	2	9	158	18	176	3.60e-12	65.5
MS.gene77939.t1	MTR_5g031000	30.500	200	111	7	9	186	2	195	4.37e-12	64.7
MS.gene77939.t1	MTR_1g053070	42.647	68	39	0	9	76	2	69	6.41e-12	64.7
MS.gene77939.t1	MTR_2g009890	41.176	68	40	0	9	76	2	69	8.06e-12	64.7
MS.gene77939.t1	MTR_2g017865	42.647	68	39	0	9	76	17	84	1.01e-11	64.3
MS.gene77939.t1	MTR_5g032520	39.706	68	41	0	9	76	2	69	1.11e-11	63.5
MS.gene77939.t1	MTR_5g032150	39.706	68	41	0	9	76	2	69	1.33e-11	63.5
MS.gene77939.t1	MTR_4g051538	23.223	211	135	6	1	204	1	191	1.50e-11	63.5
MS.gene77939.t1	MTR_0003s0590	36.585	82	52	0	9	90	2	83	1.62e-11	63.5
MS.gene77939.t1	MTR_1g029670	44.776	67	36	1	9	75	2	67	1.72e-11	62.4
MS.gene77939.t1	MTR_4g109830	42.254	71	38	2	7	76	2	70	1.84e-11	63.2
MS.gene77939.t1	MTR_5g021270	45.714	70	36	1	9	76	2	71	1.93e-11	63.2
MS.gene77939.t1	MTR_5g021270	45.714	70	36	1	9	76	2	71	2.01e-11	63.2
MS.gene77939.t1	MTR_6g464720	40.476	84	44	3	7	86	2	83	2.17e-11	63.2
MS.gene77939.t1	MTR_8g097090	34.000	100	62	2	9	104	2	101	2.44e-11	62.8
MS.gene77939.t1	MTR_2g461710	48.214	56	29	0	9	64	2	57	2.59e-11	58.5
MS.gene77939.t1	MTR_5g066960	42.105	57	33	0	9	65	2	58	2.93e-11	58.5
MS.gene77939.t1	MTR_3g088615	44.776	67	36	1	9	75	2	67	2.95e-11	61.6
MS.gene77939.t1	MTR_3g084980	32.407	108	69	2	9	112	2	109	3.32e-11	62.4
MS.gene77939.t1	MTR_1g101970	41.176	68	40	0	9	76	2	69	3.37e-11	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77939.t1	AT5G60440	49.405	168	82	2	4	168	2	169	5.14e-46	157
MS.gene77939.t1	AT1G01530	42.771	166	88	3	4	166	2	163	1.26e-33	123
MS.gene77939.t1	AT2G24840	42.500	160	83	3	9	163	63	218	1.37e-33	124
MS.gene77939.t1	AT1G65360	34.513	226	124	6	4	211	2	221	1.44e-32	120
MS.gene77939.t1	AT4G36590	39.157	166	89	4	4	160	2	164	9.57e-28	108
MS.gene77939.t1	AT3G04100	29.341	167	104	4	2	159	7	168	3.27e-19	84.3
MS.gene77939.t1	AT3G66656	36.885	122	67	2	9	123	2	120	2.53e-18	81.3
MS.gene77939.t1	AT2G34440	37.168	113	70	1	9	121	2	113	9.27e-18	79.3
MS.gene77939.t1	AT1G72350	50.685	73	34	1	9	81	43	113	7.06e-17	78.2
MS.gene77939.t1	AT1G28460	36.735	98	62	0	5	102	4	101	2.56e-14	70.1
MS.gene77939.t1	AT4G09960	39.535	86	51	1	9	93	2	87	3.58e-14	70.5
MS.gene77939.t1	AT4G09960	39.535	86	51	1	9	93	3	88	4.38e-14	70.5
MS.gene77939.t1	AT4G09960	39.535	86	51	1	9	93	2	87	4.53e-14	70.5
MS.gene77939.t1	AT4G09960	39.535	86	51	1	9	93	12	97	5.03e-14	70.5
MS.gene77939.t1	AT4G09960	40.000	90	51	2	5	93	26	113	6.97e-14	70.5
MS.gene77939.t1	AT2G45660	45.588	68	37	0	10	77	3	70	7.67e-14	68.2
MS.gene77939.t1	AT4G09960	40.000	90	51	2	5	93	95	182	1.51e-13	70.5
MS.gene77939.t1	AT2G45660	45.588	68	37	0	10	77	3	70	1.59e-13	68.6
MS.gene77939.t1	AT4G18960	33.333	114	74	1	2	113	11	124	1.61e-13	69.3
MS.gene77939.t1	AT1G22130	36.957	92	57	1	9	99	2	93	1.94e-13	70.1
MS.gene77939.t1	AT1G77980	38.202	89	54	1	9	96	2	90	3.02e-13	69.3
MS.gene77939.t1	AT3G54340	44.286	70	37	1	9	76	2	71	5.31e-13	67.4
MS.gene77939.t1	AT2G45650	42.647	68	39	0	9	76	2	69	5.98e-13	67.8
MS.gene77939.t1	AT3G57230	39.706	68	41	0	9	76	2	69	1.60e-12	64.3
MS.gene77939.t1	AT4G22950	43.284	67	38	0	10	76	3	69	1.78e-12	65.1
MS.gene77939.t1	AT2G22540	42.647	68	39	0	9	76	2	69	2.19e-12	65.9
MS.gene77939.t1	AT2G22540	42.647	68	39	0	9	76	2	69	2.19e-12	65.9
MS.gene77939.t1	AT5G51870	46.269	67	36	0	10	76	3	69	3.13e-12	64.3
MS.gene77939.t1	AT5G51870	46.269	67	36	0	10	76	3	69	3.34e-12	64.7
MS.gene77939.t1	AT4G22950	43.284	67	38	0	10	76	3	69	3.40e-12	65.1
MS.gene77939.t1	AT4G22950	43.284	67	38	0	10	76	3	69	3.40e-12	65.1
MS.gene77939.t1	AT2G42830	42.667	75	41	1	2	76	12	84	3.82e-12	65.5
MS.gene77939.t1	AT2G42830	42.667	75	41	1	2	76	12	84	4.39e-12	65.1
MS.gene77939.t1	AT3G58780	42.667	75	41	1	2	76	12	84	4.47e-12	65.1
MS.gene77939.t1	AT1G77950	34.831	89	57	1	9	96	2	90	4.50e-12	65.1
MS.gene77939.t1	AT1G77950	34.831	89	57	1	9	96	2	90	4.50e-12	65.1
MS.gene77939.t1	AT1G77950	34.831	89	57	1	9	96	2	90	4.50e-12	65.1
MS.gene77939.t1	AT1G77950	34.831	89	57	1	9	96	2	90	4.50e-12	65.1
MS.gene77939.t1	AT4G37940	41.176	68	40	0	9	76	2	69	4.59e-12	64.7
MS.gene77939.t1	AT3G58780	42.667	75	41	1	2	76	18	90	5.12e-12	65.1
MS.gene77939.t1	AT4G37940	39.506	81	42	1	9	89	2	75	5.20e-12	64.7
MS.gene77939.t1	AT4G37940	41.176	68	40	0	9	76	2	69	5.32e-12	63.5
MS.gene77939.t1	AT5G51870	46.269	67	36	0	10	76	13	79	5.92e-12	64.7
MS.gene77939.t1	AT5G51870	46.269	67	36	0	10	76	3	69	5.99e-12	64.3
MS.gene77939.t1	AT4G24540	42.647	68	39	0	9	76	2	69	6.44e-12	64.3
MS.gene77939.t1	AT1G17310	32.934	167	71	4	8	169	47	177	7.14e-12	63.9
MS.gene77939.t1	AT3G57230	39.706	68	41	0	9	76	2	69	7.70e-12	64.3
MS.gene77939.t1	AT3G57230	39.706	68	41	0	9	76	2	69	7.70e-12	64.3
MS.gene77939.t1	AT5G51870	46.269	67	36	0	10	76	26	92	7.88e-12	63.5
MS.gene77939.t1	AT1G17310	32.738	168	72	4	8	170	58	189	7.90e-12	64.3
MS.gene77939.t1	AT5G10140	43.478	69	38	1	9	76	2	70	8.46e-12	62.8
MS.gene77939.t1	AT3G58780	42.667	75	41	1	2	76	12	84	8.50e-12	64.7
MS.gene77939.t1	AT1G29962	33.663	101	64	1	5	102	4	104	8.78e-12	63.2
MS.gene77939.t1	AT5G13790	44.118	68	38	0	9	76	2	69	8.98e-12	64.7
MS.gene77939.t1	AT5G13790	44.118	68	38	0	9	76	2	69	8.99e-12	64.7
MS.gene77939.t1	AT3G61120	44.118	68	38	0	9	76	2	69	9.15e-12	64.3
MS.gene77939.t1	AT5G10140	43.478	69	38	1	9	76	2	70	9.86e-12	63.2
MS.gene77939.t1	AT5G62165	41.791	67	39	0	10	76	3	69	9.89e-12	61.2
MS.gene77939.t1	AT5G10140	43.478	69	38	1	9	76	2	70	1.16e-11	63.2
MS.gene77939.t1	AT1G26310	45.070	71	36	2	7	76	2	70	1.31e-11	63.9
MS.gene77939.t1	AT5G62165	41.791	67	39	0	10	76	3	69	1.35e-11	62.0
MS.gene77939.t1	AT5G62165	41.791	67	39	0	10	76	3	69	1.35e-11	62.0
MS.gene77939.t1	AT5G62165	41.791	67	39	0	10	76	3	69	1.35e-11	62.0
MS.gene77939.t1	AT5G62165	41.791	67	39	0	10	76	3	69	1.35e-11	62.0
MS.gene77939.t1	AT5G62165	41.791	67	39	0	10	76	3	69	1.35e-11	62.0
MS.gene77939.t1	AT5G62165	41.791	67	39	0	10	76	3	69	1.38e-11	62.0
MS.gene77939.t1	AT5G62165	41.791	67	39	0	10	76	3	69	1.38e-11	62.0
MS.gene77939.t1	AT5G62165	41.791	67	39	0	10	76	3	69	1.38e-11	62.0
MS.gene77939.t1	AT5G10140	43.478	69	38	1	9	76	2	70	1.63e-11	62.4
MS.gene77939.t1	AT3G57390	41.176	68	40	0	9	76	2	69	2.82e-11	62.4
MS.gene77939.t1	AT1G28450	25.143	175	119	4	5	168	4	177	3.40e-11	61.6
MS.gene77939.t1	AT5G62165	41.791	67	39	0	10	76	3	69	4.52e-11	61.6
MS.gene77939.t1	AT5G62165	41.791	67	39	0	10	76	3	69	4.52e-11	61.6
MS.gene77939.t1	AT5G60910	35.514	107	57	2	9	103	2	108	4.95e-11	62.0
MS.gene77939.t1	AT3G57390	41.176	68	40	0	9	76	2	69	5.83e-11	62.0

Find 59 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAATTATTCAAGAAGATTT+TGG	0.197704	3.2:-21480984	MS.gene77939:CDS
TTTAATTGGCTTCAACAATA+TGG	0.202381	3.2:-21480774	MS.gene77939:CDS
TCATAGATTGCTATCTCTTT+GGG	0.241640	3.2:-21481213	MS.gene77939:CDS
ATCTCAGTTTGTTGAGGATT+TGG	0.255732	3.2:+21480839	None:intergenic
ATGTTGCAACCTCATTTAAT+TGG	0.256996	3.2:-21480788	MS.gene77939:CDS
AAACCATTACCAACAGAAAA+TGG	0.261012	3.2:+21480890	None:intergenic
GTCATAGATTGCTATCTCTT+TGG	0.261827	3.2:-21481214	MS.gene77939:CDS
TACGAGTCTAATCATATCTT+TGG	0.269074	3.2:-21480605	MS.gene77939:CDS
TGGTGGGCTTGTCCGATTAA+TGG	0.300675	3.2:-21481025	MS.gene77939:CDS
TAGAATTTCAATAGAATATC+CGG	0.329833	3.2:+21480730	None:intergenic
AATATGGGAGGAGGAGGATA+TGG	0.331968	3.2:-21480758	MS.gene77939:CDS
AGGCGTTCAAGGCTCAATTT+TGG	0.334747	3.2:-21481045	MS.gene77939:CDS
ATGAGAAATTGAATTGGTTA+TGG	0.337177	3.2:+21480660	None:intergenic
GGTGGGCTTGTCCGATTAAT+GGG	0.369852	3.2:-21481024	MS.gene77939:CDS
GTCCGATTAATGGGATGAAT+AGG	0.377904	3.2:-21481015	MS.gene77939:CDS
GCCCTATTCATCCCATTAAT+CGG	0.382765	3.2:+21481013	None:intergenic
TTAATTGGCTTCAACAATAT+GGG	0.383325	3.2:-21480773	MS.gene77939:CDS
TTGTTGCTCATCATGTTGAT+AGG	0.402553	3.2:-21480946	MS.gene77939:CDS
GTGAGCCTCAATGAATTCTA+TGG	0.413803	3.2:+21481168	None:intergenic
ATAATAGAGAGGGATATTAG+AGG	0.419819	3.2:+21480865	None:intergenic
TCCGATTAATGGGATGAATA+GGG	0.426477	3.2:-21481014	MS.gene77939:CDS
ATTAAATGAGGTTGCAACAT+AGG	0.428043	3.2:+21480791	None:intergenic
TCTATGACCGTATCAACATT+TGG	0.448129	3.2:+21481229	None:intergenic
ACCTTTCCTTGCGCTTGACA+TGG	0.477663	3.2:+21481429	None:intergenic
AGCATTGAGCTCACACACTT+TGG	0.483043	3.2:+21481138	None:intergenic
GCATAGATGAGAAATTGAAT+TGG	0.484824	3.2:+21480654	None:intergenic
ATATTAGATGGCAGAGGAAA+GGG	0.484969	3.2:-21480568	MS.gene77939:CDS
TTAAATGAGGTTGCAACATA+GGG	0.493554	3.2:+21480792	None:intergenic
TTCATACAACACAAGTAAAG+TGG	0.501083	3.2:+21480686	None:intergenic
AGAATTTCAATAGAATATCC+GGG	0.513438	3.2:+21480731	None:intergenic
CGGTCATCCAAATGTTGATA+CGG	0.514501	3.2:-21481236	MS.gene77939:CDS
AGAAACATCTACACCACAAA+GGG	0.515799	3.2:+21481297	None:intergenic
TGTATTCTCACCTAGTGAAA+AGG	0.518205	3.2:-21481269	MS.gene77939:CDS
GCTCACACACTTTGGCTCTA+CGG	0.521890	3.2:+21481146	None:intergenic
GTTGATAGGAGTGCAATTCA+AGG	0.523826	3.2:-21480932	MS.gene77939:CDS
AAAGTTTGAGTTTCAAGAGA+GGG	0.530083	3.2:+21480632	None:intergenic
TTCAACAATATGGGAGGAGG+AGG	0.541923	3.2:-21480764	MS.gene77939:CDS
TTGTTGAAGCCAATTAAATG+AGG	0.551491	3.2:+21480779	None:intergenic
TGAACTATATTAGATGGCAG+AGG	0.554094	3.2:-21480574	MS.gene77939:CDS
GAAAACATCTCAGTTTGTTG+AGG	0.554882	3.2:+21480833	None:intergenic
GCTTTGAGAAAATCACTTGG+AGG	0.556997	3.2:+21481359	None:intergenic
TATATTAGATGGCAGAGGAA+AGG	0.558581	3.2:-21480569	MS.gene77939:CDS
GGCTTCAACAATATGGGAGG+AGG	0.561767	3.2:-21480767	MS.gene77939:CDS
GGATTTGGCTGATAATAGAG+AGG	0.582271	3.2:+21480854	None:intergenic
AATCAACAACACATCGGATG+AGG	0.592418	3.2:-21481104	MS.gene77939:CDS
ACCATGTCAAGCGCAAGGAA+AGG	0.599500	3.2:-21481430	MS.gene77939:CDS
ATTGGCTTCAACAATATGGG+AGG	0.605743	3.2:-21480770	MS.gene77939:CDS
CATAGATTGCTATCTCTTTG+GGG	0.615069	3.2:-21481212	MS.gene77939:CDS
GACGCTTTGAGAAAATCACT+TGG	0.615395	3.2:+21481356	None:intergenic
AAAAGTTTGAGTTTCAAGAG+AGG	0.623898	3.2:+21480631	None:intergenic
CTTCAACCATGTCAAGCGCA+AGG	0.629141	3.2:-21481435	None:intergenic
TCAAGCGCAAGGAAAGGTCG+TGG	0.632671	3.2:-21481424	MS.gene77939:CDS
GATTTGGCTGATAATAGAGA+GGG	0.634728	3.2:+21480855	None:intergenic
GAATTTCAATAGAATATCCG+GGG	0.640180	3.2:+21480732	None:intergenic
GAGAAACATCTACACCACAA+AGG	0.651442	3.2:+21481296	None:intergenic
GATTCAAATCAACAACACAT+CGG	0.687185	3.2:-21481110	MS.gene77939:CDS
TAGCACCATAGAATTCATTG+AGG	0.695216	3.2:-21481173	MS.gene77939:CDS
AATTTCAATAGAATATCCGG+GGG	0.699943	3.2:+21480733	None:intergenic
ATTTCAATAGAATATCCGGG+GGG	0.825379	3.2:+21480734	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAGAATTTCAATAGAATATC+CGG	+	chr3.2:21481265-21481284	None:intergenic	20.0%
!!!	TCATTTTGAACTATATTAGA+TGG	-	chr3.2:21481412-21481431	MS.gene77939:CDS	20.0%
!!!	TGAATTATTCAAGAAGATTT+TGG	-	chr3.2:21481008-21481027	MS.gene77939:CDS	20.0%
!	AGAATTTCAATAGAATATCC+GGG	+	chr3.2:21481264-21481283	None:intergenic	25.0%
!	ATAGGGTTATGAAAAATTTG+TGG	+	chr3.2:21481186-21481205	None:intergenic	25.0%
!	ATGAGAAATTGAATTGGTTA+TGG	+	chr3.2:21481335-21481354	None:intergenic	25.0%
!!	AAAGAAAATACCTTTTCACT+AGG	+	chr3.2:21480736-21480755	None:intergenic	25.0%
!!	ATTATTCAAGAAGATTTTGG+TGG	-	chr3.2:21481011-21481030	MS.gene77939:CDS	25.0%
!!	CAAATTTTTCCATTTTCTGT+TGG	-	chr3.2:21481093-21481112	MS.gene77939:CDS	25.0%
!!	TTAATTGGCTTCAACAATAT+GGG	-	chr3.2:21481219-21481238	MS.gene77939:CDS	25.0%
!!	TTTAATTGGCTTCAACAATA+TGG	-	chr3.2:21481218-21481237	MS.gene77939:CDS	25.0%
!!!	AGTGAAAAGGTATTTTCTTT+CGG	-	chr3.2:21480736-21480755	MS.gene77939:CDS	25.0%
	AAACCATTACCAACAGAAAA+TGG	+	chr3.2:21481105-21481124	None:intergenic	30.0%
	AATTTCAATAGAATATCCGG+GGG	+	chr3.2:21481262-21481281	None:intergenic	30.0%
	ATAATAGAGAGGGATATTAG+AGG	+	chr3.2:21481130-21481149	None:intergenic	30.0%
	ATGTTGCAACCTCATTTAAT+TGG	-	chr3.2:21481204-21481223	MS.gene77939:CDS	30.0%
	ATTAAATGAGGTTGCAACAT+AGG	+	chr3.2:21481204-21481223	None:intergenic	30.0%
	GAATTTCAATAGAATATCCG+GGG	+	chr3.2:21481263-21481282	None:intergenic	30.0%
	GATTCAAATCAACAACACAT+CGG	-	chr3.2:21480882-21480901	MS.gene77939:CDS	30.0%
	GCATAGATGAGAAATTGAAT+TGG	+	chr3.2:21481341-21481360	None:intergenic	30.0%
	TACGAGTCTAATCATATCTT+TGG	-	chr3.2:21481387-21481406	MS.gene77939:CDS	30.0%
	TCATAGATTGCTATCTCTTT+GGG	-	chr3.2:21480779-21480798	MS.gene77939:CDS	30.0%
	TTAAATGAGGTTGCAACATA+GGG	+	chr3.2:21481203-21481222	None:intergenic	30.0%
	TTCATACAACACAAGTAAAG+TGG	+	chr3.2:21481309-21481328	None:intergenic	30.0%
	TTGTTGAAGCCAATTAAATG+AGG	+	chr3.2:21481216-21481235	None:intergenic	30.0%
!	AAAAGTTTGAGTTTCAAGAG+AGG	+	chr3.2:21481364-21481383	None:intergenic	30.0%
!	AAAGTTTGAGTTTCAAGAGA+GGG	+	chr3.2:21481363-21481382	None:intergenic	30.0%
!!	TTTCCATTTTCTGTTGGTAA+TGG	-	chr3.2:21481099-21481118	MS.gene77939:CDS	30.0%
!!!	TTTTTTTCGTCTCGATCTTT+TGG	+	chr3.2:21480597-21480616	None:intergenic	30.0%
	AGAAACATCTACACCACAAA+GGG	+	chr3.2:21480698-21480717	None:intergenic	35.0%
	ATATTAGATGGCAGAGGAAA+GGG	-	chr3.2:21481424-21481443	MS.gene77939:CDS	35.0%
	ATTTCAATAGAATATCCGGG+GGG	+	chr3.2:21481261-21481280	None:intergenic	35.0%
	CATAGATTGCTATCTCTTTG+GGG	-	chr3.2:21480780-21480799	MS.gene77939:CDS	35.0%
	GATTTGGCTGATAATAGAGA+GGG	+	chr3.2:21481140-21481159	None:intergenic	35.0%
	GTCATAGATTGCTATCTCTT+TGG	-	chr3.2:21480778-21480797	MS.gene77939:CDS	35.0%
	TAGCACCATAGAATTCATTG+AGG	-	chr3.2:21480819-21480838	MS.gene77939:CDS	35.0%
	TATATTAGATGGCAGAGGAA+AGG	-	chr3.2:21481423-21481442	MS.gene77939:CDS	35.0%
	TCCGATTAATGGGATGAATA+GGG	-	chr3.2:21480978-21480997	MS.gene77939:CDS	35.0%
	TCTATGACCGTATCAACATT+TGG	+	chr3.2:21480766-21480785	None:intergenic	35.0%
	TGAACTATATTAGATGGCAG+AGG	-	chr3.2:21481418-21481437	MS.gene77939:CDS	35.0%
	TGTATTCTCACCTAGTGAAA+AGG	-	chr3.2:21480723-21480742	MS.gene77939:CDS	35.0%
	TTGTTGCTCATCATGTTGAT+AGG	-	chr3.2:21481046-21481065	MS.gene77939:CDS	35.0%
!!	ATCTCAGTTTGTTGAGGATT+TGG	+	chr3.2:21481156-21481175	None:intergenic	35.0%
!!	GAAAACATCTCAGTTTGTTG+AGG	+	chr3.2:21481162-21481181	None:intergenic	35.0%
!!!	TCTATGGTGCTATTGTTTTG+CGG	+	chr3.2:21480811-21480830	None:intergenic	35.0%
	AATCAACAACACATCGGATG+AGG	-	chr3.2:21480888-21480907	MS.gene77939:CDS	40.0%
	CGGTCATCCAAATGTTGATA+CGG	-	chr3.2:21480756-21480775	MS.gene77939:CDS	40.0%
	GAGAAACATCTACACCACAA+AGG	+	chr3.2:21480699-21480718	None:intergenic	40.0%
	GCCCTATTCATCCCATTAAT+CGG	+	chr3.2:21480982-21481001	None:intergenic	40.0%
	GGATTTGGCTGATAATAGAG+AGG	+	chr3.2:21481141-21481160	None:intergenic	40.0%
	GTCCGATTAATGGGATGAAT+AGG	-	chr3.2:21480977-21480996	MS.gene77939:CDS	40.0%
	GTGAGCCTCAATGAATTCTA+TGG	+	chr3.2:21480827-21480846	None:intergenic	40.0%
!	ATTGGCTTCAACAATATGGG+AGG	-	chr3.2:21481222-21481241	MS.gene77939:CDS	40.0%
!	GTTGATAGGAGTGCAATTCA+AGG	-	chr3.2:21481060-21481079	MS.gene77939:CDS	40.0%
!!	GACGCTTTGAGAAAATCACT+TGG	+	chr3.2:21480639-21480658	None:intergenic	40.0%
!!	GCTTTGAGAAAATCACTTGG+AGG	+	chr3.2:21480636-21480655	None:intergenic	40.0%
!!	GTTCAAGGCTCAATTTTGGT+GGG	-	chr3.2:21480951-21480970	MS.gene77939:CDS	40.0%
!!	TGAGTTGAGTCTTTTGTGCA+AGG	-	chr3.2:21480927-21480946	MS.gene77939:CDS	40.0%
!!!	ATTTTCTCAAAGCGTCGTAG+CGG	-	chr3.2:21480643-21480662	MS.gene77939:CDS	40.0%
	AATATGGGAGGAGGAGGATA+TGG	-	chr3.2:21481234-21481253	MS.gene77939:CDS	45.0%
	TCGGATGAGGAGAAAAAGCA+TGG	-	chr3.2:21480901-21480920	MS.gene77939:CDS	45.0%
	TTCAACAATATGGGAGGAGG+AGG	-	chr3.2:21481228-21481247	MS.gene77939:CDS	45.0%
!	ACTGAGTTTTGCTCCCTTTG+TGG	-	chr3.2:21480682-21480701	MS.gene77939:CDS	45.0%
!	AGCATTGAGCTCACACACTT+TGG	+	chr3.2:21480857-21480876	None:intergenic	45.0%
!	AGGCGTTCAAGGCTCAATTT+TGG	-	chr3.2:21480947-21480966	MS.gene77939:CDS	45.0%
!	CGTTCAAGGCTCAATTTTGG+TGG	-	chr3.2:21480950-21480969	MS.gene77939:CDS	45.0%
!!	TCTTTTGTGCAAGGCGTTCA+AGG	-	chr3.2:21480936-21480955	MS.gene77939:CDS	45.0%
!!!	ATGGTGCTATTGTTTTGCGG+TGG	+	chr3.2:21480808-21480827	None:intergenic	45.0%
!!!	TGGTGCTATTGTTTTGCGGT+GGG	+	chr3.2:21480807-21480826	None:intergenic	45.0%
!!!	TTTTCTCAAAGCGTCGTAGC+GGG	-	chr3.2:21480644-21480663	MS.gene77939:CDS	45.0%
	GGCTTCAACAATATGGGAGG+AGG	-	chr3.2:21481225-21481244	MS.gene77939:CDS	50.0%
!	GGTGGGCTTGTCCGATTAAT+GGG	-	chr3.2:21480968-21480987	MS.gene77939:CDS	50.0%
!!	GCTCACACACTTTGGCTCTA+CGG	+	chr3.2:21480849-21480868	None:intergenic	50.0%
!!	TGGTGGGCTTGTCCGATTAA+TGG	-	chr3.2:21480967-21480986	MS.gene77939:CDS	50.0%
	TCAAGCGCAAGGAAAGGTCG+TGG	-	chr3.2:21480568-21480587	MS.gene77939:CDS	55.0%
	GGAGGAGGATATGGACCCCC+CGG	-	chr3.2:21481243-21481262	MS.gene77939:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	21480565	21481449	21480565	ID=MS.gene77939
chr3.2	mRNA	21480565	21481449	21480565	ID=MS.gene77939.t1;Parent=MS.gene77939
chr3.2	exon	21480565	21481449	21480565	ID=MS.gene77939.t1.exon1;Parent=MS.gene77939.t1
chr3.2	CDS	21480565	21481449	21480565	ID=cds.MS.gene77939.t1;Parent=MS.gene77939.t1

Gene Sequence

>MS.gene77939

ATGTCAAGCGCAAGGAAAGGTCGTGGTCGCCAAAAGATCGAGACGAAAAAAATGAGCAACGAGAGCAACCTCCAAGTGATTTTCTCAAAGCGTCGTAGCGGGCTCTTCAAGAAAGTCACTGAGTTTTGCTCCCTTTGTGGTGTAGATGTTTCTCTCGTTGTATTCTCACCTAGTGAAAAGGTATTTTCTTTCGGTCATCCAAATGTTGATACGGTCATAGATTGCTATCTCTTTGGGGTCCCACCGCAAAACAATAGCACCATAGAATTCATTGAGGCTCACCGTAGAGCCAAAGTGTGTGAGCTCAATGCTGAGTTGATTCAAATCAACAACACATCGGATGAGGAGAAAAAGCATGGTGATGAGTTGAGTCTTTTGTGCAAGGCGTTCAAGGCTCAATTTTGGTGGGCTTGTCCGATTAATGGGATGAATAGGGCTCAACTTGAATTATTCAAGAAGATTTTGGTGGAGCTTAAGAAACTTGTTGCTCATCATGTTGATAGGAGTGCAATTCAAGGTGCTTTTGCTCAAATTTTTCCATTTTCTGTTGGTAATGGTTTATCCTCTAATATCCCTCTCTATTATCAGCCAAATCCTCAACAAACTGAGATGTTTTCGCCACAAATTTTTCATAACCCTATGTTGCAACCTCATTTAATTGGCTTCAACAATATGGGAGGAGGAGGATATGGACCCCCCGGATATTCTATTGAAATTCTAGAGTCATTAAGTTCAGCTTCGCCACTTTACTTGTGTTGTATGAAAAGCCATAACCAATTCAATTTCTCATCTATGCCCTCTCTTGAAACTCAAACTTTTGTTTACGAGTCTAATCATATCTTTGGTATCATTTTGAACTATATTAGATGGCAGAGGAAAGGGTAG

Protein sequence

>MS.gene77939.t1

MSSARKGRGRQKIETKKMSNESNLQVIFSKRRSGLFKKVTEFCSLCGVDVSLVVFSPSEKVFSFGHPNVDTVIDCYLFGVPPQNNSTIEFIEAHRRAKVCELNAELIQINNTSDEEKKHGDELSLLCKAFKAQFWWACPINGMNRAQLELFKKILVELKKLVAHHVDRSAIQGAFAQIFPFSVGNGLSSNIPLYYQPNPQQTEMFSPQIFHNPMLQPHLIGFNNMGGGGYGPPGYSIEILESLSSASPLYLCCMKSHNQFNFSSMPSLETQTFVYESNHIFGIILNYIRWQRKG