AlfalfaGEDB | MS.gene78192

M. sativa cultivar XinJiangDaYe / MS.gene78192

KEGG: K06693
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF13180.9 PF18265.4

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78192.t1	XP_013464039.1	94.6	221	6	1	1	221	1	215	3.30E-107	397.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78192.t1	Q9VFS8	29.8	225	132	5	3	221	4	208	2.1e-17	90.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78192.t1	A0A072V7P5	94.6	221	6	1	1	221	1	215	2.4e-107	397.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene78192	MS.gene44130	PPI
MS.gene78192	MS.gene005258	PPI
MS.gene005257	MS.gene78192	PPI
MS.gene78192	MS.gene043161	PPI
MS.gene78192	MS.gene056063	PPI
MS.gene93918	MS.gene78192	PPI
MS.gene97064	MS.gene78192	PPI
MS.gene48614	MS.gene78192	PPI
MS.gene78192	MS.gene40879	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78192.t1	MTR_2g460780	97.285	221	6	0	1	221	1	221	1.67e-158	438
MS.gene78192.t1	MTR_2g460780	94.570	221	6	1	1	221	1	215	3.28e-151	419
MS.gene78192.t1	MTR_2g460780	91.403	221	5	1	1	221	1	207	1.76e-144	402

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78192.t1	AT5G57950	61.883	223	81	3	1	221	1	221	5.24e-93	273
MS.gene78192.t1	AT5G57950	61.883	223	81	3	1	221	30	250	1.59e-92	272

Find 56 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATAACAAATAACAAAATAAT+TGG	0.204859	2.2:-32996764	None:intergenic
TTGGCAAGGCAGAGGTTTAC+TGG	0.266830	2.2:+32996726	MS.gene78192:CDS
CCTGGCGCCCCTGGACTTTC+CGG	0.273188	2.2:+32995686	MS.gene78192:CDS
TTGTTTGACCCATGCTCGAC+TGG	0.343385	2.2:-32996645	None:intergenic
TCTCAATTCCCTGGCGCCCC+TGG	0.352289	2.2:+32995677	MS.gene78192:CDS
CTCAGAATCAATTAGATTGC+CGG	0.364511	2.2:-32995705	None:intergenic
AACAAGTGGAACGTCGATAT+CGG	0.371561	2.2:-32995829	None:intergenic
AGCAGAAACAAGATCTCTAA+TGG	0.389792	2.2:+32995604	MS.gene78192:CDS
ACATCCACATCCATCGAGTT+GGG	0.410980	2.2:-32996475	None:intergenic
ACCCATGCTCGACTGGGCCT+CGG	0.418523	2.2:-32996638	None:intergenic
CCGGAAAGTCCAGGGGCGCC+AGG	0.421638	2.2:-32995686	None:intergenic
GCTCGTCTTTCTCAATTCCC+TGG	0.439681	2.2:+32995668	MS.gene78192:CDS
TTCCGAGGCCCAGTCGAGCA+TGG	0.452146	2.2:+32996636	MS.gene78192:CDS
TGAAAATTTGCTCCATAGGT+TGG	0.465291	2.2:+32996612	MS.gene78192:CDS
ATCTCGTCAACCACTGCAAA+AGG	0.466046	2.2:-32996511	None:intergenic
AAATTTGGCAACGTCGAAGC+AGG	0.480789	2.2:+32996589	MS.gene78192:CDS
TAACAAATAACAAAATAATT+GGG	0.487430	2.2:-32996763	None:intergenic
ATTAGATTGCCGGAAAGTCC+AGG	0.487468	2.2:-32995695	None:intergenic
CCTCTGCCTTGCCAAGTCCT+AGG	0.496210	2.2:-32996718	None:intergenic
CGGCAATCTAATTGATTCTG+AGG	0.497483	2.2:+32995706	MS.gene78192:CDS
TTCCGCTAAGACAATGATGA+TGG	0.500406	2.2:+32996376	MS.gene78192:intron
GTAGAAGATGGTTTACAGCT+TGG	0.500978	2.2:+32996553	MS.gene78192:CDS
GGGCCTCGGAAGCCAACCTA+TGG	0.501119	2.2:-32996624	None:intergenic
CAGGTGAAAATTTGCTCCAT+AGG	0.501246	2.2:+32996608	MS.gene78192:CDS
TGGCAAGGCAGAGGTTTACT+GGG	0.504955	2.2:+32996727	MS.gene78192:CDS
CTCGATGGATGTGGATGTCT+TGG	0.513868	2.2:+32996480	MS.gene78192:CDS
GACATGCAGACCTCATCTAC+TGG	0.518074	2.2:+32996403	MS.gene78192:CDS
ATTAATCTTATCAGTTATGT+CGG	0.522206	2.2:-32995895	None:intergenic
TCCGAGGCCCAGTCGAGCAT+GGG	0.530910	2.2:+32996637	MS.gene78192:CDS
GACATCCACATCCATCGAGT+TGG	0.533268	2.2:-32996476	None:intergenic
CCTTGCCTCATGATGACAAC+AGG	0.536038	2.2:-32996670	None:intergenic
GCTCCATAGGTTGGCTTCCG+AGG	0.537445	2.2:+32996621	MS.gene78192:CDS
AATAACAAAATAATTGGGAC+AGG	0.549596	2.2:-32996758	None:intergenic
TTTATGATTTCCAAGTCTTG+AGG	0.549764	2.2:-32995940	None:intergenic
TGTTTGACCCATGCTCGACT+GGG	0.552241	2.2:-32996644	None:intergenic
GCATCACCAGCGGTAGAAGA+TGG	0.559452	2.2:+32996541	MS.gene78192:CDS
CGTCGTTCAGCTCGAACAAG+TGG	0.560165	2.2:-32995843	None:intergenic
CCTAGGACTTGGCAAGGCAG+AGG	0.560284	2.2:+32996718	MS.gene78192:CDS
TGTAAACCATCTTCTACCGC+TGG	0.569610	2.2:-32996547	None:intergenic
CAGAGTAGAAGAAGAATGGT+GGG	0.571601	2.2:+32995569	None:intergenic
CGGAAAGTCCAGGGGCGCCA+GGG	0.574777	2.2:-32995685	None:intergenic
TAACTGTTACACCTAGGACT+TGG	0.577662	2.2:+32996707	MS.gene78192:CDS
CATCCACATCCATCGAGTTG+GGG	0.579168	2.2:-32996474	None:intergenic
TTAGATTGCCGGAAAGTCCA+GGG	0.588856	2.2:-32995694	None:intergenic
CAAGATCTCTAATGGATAAG+CGG	0.592560	2.2:+32995612	MS.gene78192:CDS
CAATACCTGTTGTCATCATG+AGG	0.605657	2.2:+32996665	MS.gene78192:CDS
AACAGTCTCTCCAGTAGATG+AGG	0.614905	2.2:-32996413	None:intergenic
CCTGTTGTCATCATGAGGCA+AGG	0.626933	2.2:+32996670	MS.gene78192:CDS
GTTACACCTAGGACTTGGCA+AGG	0.647181	2.2:+32996712	MS.gene78192:CDS
CTCACAAGGACATTCTGCAA+TGG	0.656644	2.2:-32996445	None:intergenic
ATCCACATCCATCGAGTTGG+GGG	0.663720	2.2:-32996473	None:intergenic
TCAACTTAACTGTTACACCT+AGG	0.680007	2.2:+32996701	MS.gene78192:CDS
TAGATTGCCGGAAAGTCCAG+GGG	0.688081	2.2:-32995693	None:intergenic
GGCAAGGCAGAGGTTTACTG+GGG	0.698130	2.2:+32996728	MS.gene78192:CDS
GATATCAGATGCATCACCAG+CGG	0.721340	2.2:+32996531	MS.gene78192:CDS
GGAACGTCGATATCGGAACG+AGG	0.736140	2.2:-32995822	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAACAAATAACAAAATAAT+TGG	-	chr2.2:32996767-32996786	None:intergenic	10.0%
!!	TAACAAATAACAAAATAATT+GGG	-	chr2.2:32996766-32996785	None:intergenic	10.0%
!!	ACATAATTCAATGTAAACAT+AGG	+	chr2.2:32995991-32996010	MS.gene78192:intron	20.0%
!!	ATTAATCTTATCAGTTATGT+CGG	-	chr2.2:32995898-32995917	None:intergenic	20.0%
!	AATAACAAAATAATTGGGAC+AGG	-	chr2.2:32996761-32996780	None:intergenic	25.0%
!	AGATTCAAGATTTAGGAAAT+AGG	+	chr2.2:32996105-32996124	MS.gene78192:intron	25.0%
!	CTTGGAAATCATAAAAATTC+AGG	+	chr2.2:32995948-32995967	MS.gene78192:CDS	25.0%
!	GAAATCATAAAAATTCAGGT+TGG	+	chr2.2:32995952-32995971	MS.gene78192:intron	25.0%
!	TAAGATCAGATTCAAGATTT+AGG	+	chr2.2:32996098-32996117	MS.gene78192:intron	25.0%
!!!	GGAGATCAAATTTTGAAATT+TGG	+	chr2.2:32996574-32996593	MS.gene78192:CDS	25.0%
	TTCAATGTAAACATAGGTGT+CGG	+	chr2.2:32995997-32996016	MS.gene78192:intron	30.0%
	TTTATGATTTCCAAGTCTTG+AGG	-	chr2.2:32995943-32995962	None:intergenic	30.0%
!	GAAACTTCACAATCAGATTT+TGG	-	chr2.2:32996163-32996182	None:intergenic	30.0%
	AGCAGAAACAAGATCTCTAA+TGG	+	chr2.2:32995604-32995623	MS.gene78192:CDS	35.0%
	CTCAGAATCAATTAGATTGC+CGG	-	chr2.2:32995708-32995727	None:intergenic	35.0%
	GCTGTACTTGTATTACTGAA+AGG	-	chr2.2:32996193-32996212	None:intergenic	35.0%
	GTACTTGTATTACTGAAAGG+CGG	-	chr2.2:32996190-32996209	None:intergenic	35.0%
	TCAACTTAACTGTTACACCT+AGG	+	chr2.2:32996701-32996720	MS.gene78192:CDS	35.0%
!	ATTTTGCATTCCTCAAGACT+TGG	+	chr2.2:32995930-32995949	MS.gene78192:CDS	35.0%
!	CAAGATCTCTAATGGATAAG+CGG	+	chr2.2:32995612-32995631	MS.gene78192:CDS	35.0%
!	TGAAAATTTGCTCCATAGGT+TGG	+	chr2.2:32996612-32996631	MS.gene78192:CDS	35.0%
!!	ATGGATGGATTGAAAGAAGA+AGG	-	chr2.2:32995765-32995784	None:intergenic	35.0%
!!	TGGATGGATTGAAAGAAGAA+GGG	-	chr2.2:32995764-32995783	None:intergenic	35.0%
	AACAAGTGGAACGTCGATAT+CGG	-	chr2.2:32995832-32995851	None:intergenic	40.0%
	AGAGAGAGAGAGAGTTATGA+TGG	-	chr2.2:32995784-32995803	None:intergenic	40.0%
	AGCCATCATCATTGTCTTAG+CGG	-	chr2.2:32996381-32996400	None:intergenic	40.0%
	CAATACCTGTTGTCATCATG+AGG	+	chr2.2:32996665-32996684	MS.gene78192:CDS	40.0%
	GCACAAACACTTCTCGAATT+AGG	-	chr2.2:32996041-32996060	None:intergenic	40.0%
	GTAGAAGATGGTTTACAGCT+TGG	+	chr2.2:32996553-32996572	MS.gene78192:CDS	40.0%
	TAACTGTTACACCTAGGACT+TGG	+	chr2.2:32996707-32996726	MS.gene78192:CDS	40.0%
	TTCCGCTAAGACAATGATGA+TGG	+	chr2.2:32996376-32996395	MS.gene78192:intron	40.0%
!	AGAGAGAGAGTTATGATGGA+TGG	-	chr2.2:32995780-32995799	None:intergenic	40.0%
!	CAGGTGAAAATTTGCTCCAT+AGG	+	chr2.2:32996608-32996627	MS.gene78192:CDS	40.0%
!	CGGCAATCTAATTGATTCTG+AGG	+	chr2.2:32995706-32995725	MS.gene78192:CDS	40.0%
	AAATTTGGCAACGTCGAAGC+AGG	+	chr2.2:32996589-32996608	MS.gene78192:CDS	45.0%
	AACAGTCTCTCCAGTAGATG+AGG	-	chr2.2:32996416-32996435	None:intergenic	45.0%
	ACATCCACATCCATCGAGTT+GGG	-	chr2.2:32996478-32996497	None:intergenic	45.0%
	ATCTCGTCAACCACTGCAAA+AGG	-	chr2.2:32996514-32996533	None:intergenic	45.0%
	ATTAGATTGCCGGAAAGTCC+AGG	-	chr2.2:32995698-32995717	None:intergenic	45.0%
	CTCACAAGGACATTCTGCAA+TGG	-	chr2.2:32996448-32996467	None:intergenic	45.0%
	GATATCAGATGCATCACCAG+CGG	+	chr2.2:32996531-32996550	MS.gene78192:CDS	45.0%
	TCTCTCTCTCTCTCTCTCTA+GGG	+	chr2.2:32995795-32995814	MS.gene78192:intron	45.0%
	TGTAAACCATCTTCTACCGC+TGG	-	chr2.2:32996550-32996569	None:intergenic	45.0%
	TTAGATTGCCGGAAAGTCCA+GGG	-	chr2.2:32995697-32995716	None:intergenic	45.0%
!	GGTTAGTAGACCTTTTGCAG+TGG	+	chr2.2:32996501-32996520	MS.gene78192:CDS	45.0%
!!	GTAAACATAGGTGTCGGTGT+CGG	+	chr2.2:32996003-32996022	MS.gene78192:intron	45.0%
	AGTTGGGGGAAAGACTCACA+AGG	-	chr2.2:32996462-32996481	None:intergenic	50.0%
	CCTGTTGTCATCATGAGGCA+AGG	+	chr2.2:32996670-32996689	MS.gene78192:CDS	50.0%
	CCTTGCCTCATGATGACAAC+AGG	-	chr2.2:32996673-32996692	None:intergenic	50.0%
	CTCGATGGATGTGGATGTCT+TGG	+	chr2.2:32996480-32996499	MS.gene78192:CDS	50.0%
	CTCTCTCTCTCTCTCTCTCT+AGG	+	chr2.2:32995790-32995809	MS.gene78192:intron	50.0%
	GACATCCACATCCATCGAGT+TGG	-	chr2.2:32996479-32996498	None:intergenic	50.0%
	GACATGCAGACCTCATCTAC+TGG	+	chr2.2:32996403-32996422	MS.gene78192:CDS	50.0%
	GCTCGTCTTTCTCAATTCCC+TGG	+	chr2.2:32995668-32995687	MS.gene78192:CDS	50.0%
	GTTACACCTAGGACTTGGCA+AGG	+	chr2.2:32996712-32996731	MS.gene78192:CDS	50.0%
	TAGATTGCCGGAAAGTCCAG+GGG	-	chr2.2:32995696-32995715	None:intergenic	50.0%
	TGGCAAGGCAGAGGTTTACT+GGG	+	chr2.2:32996727-32996746	MS.gene78192:CDS	50.0%
	TGTTTGACCCATGCTCGACT+GGG	-	chr2.2:32996647-32996666	None:intergenic	50.0%
	TTGGCAAGGCAGAGGTTTAC+TGG	+	chr2.2:32996726-32996745	MS.gene78192:CDS	50.0%
	TTGTTTGACCCATGCTCGAC+TGG	-	chr2.2:32996648-32996667	None:intergenic	50.0%
!	CATCCACATCCATCGAGTTG+GGG	-	chr2.2:32996477-32996496	None:intergenic	50.0%
!!	ATCCACATCCATCGAGTTGG+GGG	-	chr2.2:32996476-32996495	None:intergenic	50.0%
	CGTCGTTCAGCTCGAACAAG+TGG	-	chr2.2:32995846-32995865	None:intergenic	55.0%
	GAGTCTTTCCCCCAACTCGA+TGG	+	chr2.2:32996465-32996484	MS.gene78192:CDS	55.0%
	GCATCACCAGCGGTAGAAGA+TGG	+	chr2.2:32996541-32996560	MS.gene78192:CDS	55.0%
	GGAACGTCGATATCGGAACG+AGG	-	chr2.2:32995825-32995844	None:intergenic	55.0%
	GGCAAGGCAGAGGTTTACTG+GGG	+	chr2.2:32996728-32996747	MS.gene78192:CDS	55.0%
	TTCCCCCAACTCGATGGATG+TGG	+	chr2.2:32996471-32996490	MS.gene78192:CDS	55.0%
!!	ATAGGTGTCGGTGTCGGACA+CGG	+	chr2.2:32996009-32996028	MS.gene78192:intron	55.0%
	CCTAGGACTTGGCAAGGCAG+AGG	+	chr2.2:32996718-32996737	MS.gene78192:CDS	60.0%
	CCTCTGCCTTGCCAAGTCCT+AGG	-	chr2.2:32996721-32996740	None:intergenic	60.0%
!!	GCTCCATAGGTTGGCTTCCG+AGG	+	chr2.2:32996621-32996640	MS.gene78192:CDS	60.0%
!!	TAGGTGTCGGTGTCGGACAC+GGG	+	chr2.2:32996010-32996029	MS.gene78192:intron	60.0%
	ACCCATGCTCGACTGGGCCT+CGG	-	chr2.2:32996641-32996660	None:intergenic	65.0%
	GGGCCTCGGAAGCCAACCTA+TGG	-	chr2.2:32996627-32996646	None:intergenic	65.0%
	TCCGAGGCCCAGTCGAGCAT+GGG	+	chr2.2:32996637-32996656	MS.gene78192:CDS	65.0%
	TCTCAATTCCCTGGCGCCCC+TGG	+	chr2.2:32995677-32995696	MS.gene78192:CDS	65.0%
	TTCCGAGGCCCAGTCGAGCA+TGG	+	chr2.2:32996636-32996655	MS.gene78192:CDS	65.0%
	CCTGGCGCCCCTGGACTTTC+CGG	+	chr2.2:32995686-32995705	MS.gene78192:CDS	70.0%
!!	CGGAAAGTCCAGGGGCGCCA+GGG	-	chr2.2:32995688-32995707	None:intergenic	70.0%
!!	CCGGAAAGTCCAGGGGCGCC+AGG	-	chr2.2:32995689-32995708	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	32995584	32996801	32995584	ID=MS.gene78192
chr2.2	mRNA	32995584	32996801	32995584	ID=MS.gene78192.t1;Parent=MS.gene78192
chr2.2	exon	32995584	32995727	32995584	ID=MS.gene78192.t1.exon1;Parent=MS.gene78192.t1
chr2.2	CDS	32995584	32995727	32995584	ID=cds.MS.gene78192.t1;Parent=MS.gene78192.t1
chr2.2	exon	32995816	32995969	32995816	ID=MS.gene78192.t1.exon2;Parent=MS.gene78192.t1
chr2.2	CDS	32995816	32995969	32995816	ID=cds.MS.gene78192.t1;Parent=MS.gene78192.t1
chr2.2	exon	32996386	32996801	32996386	ID=MS.gene78192.t1.exon3;Parent=MS.gene78192.t1
chr2.2	CDS	32996386	32996801	32996386	ID=cds.MS.gene78192.t1;Parent=MS.gene78192.t1

Gene Sequence

>MS.gene78192

ATGGTGGGAACGAACGTGAAAGCAGAAACAAGATCTCTAATGGATAAGCGGAGTGCGTTAGAGTCTGAGATGAACTCAATCATCGCTCGTCTTTCTCAATTCCCTGGCGCCCCTGGACTTTCCGGCAATCTAATTGATTCTGAGGTTTGTTTCTCTGCAAATTATTTCATTCAAATACCCTTCTTCTTTCAATCCATCCATCATAACTCTCTCTCTCTCTCTCTCTCTCTAGGGTTTTCCTCGTTCCGATATCGACGTTCCACTTGTTCGAGCTGAACGACGTCGTCTTGCTGAGCTGCGTAATGACTACACCGACATAACTGATAAGATTAATCAAAATATACACATTTTGCATTCCTCAAGACTTGGAAATCATAAAAATTCAGGTTGGTTTACTTCTCAAAATGACATAATTCAATGTAAACATAGGTGTCGGTGTCGGACACGGGACACACCTAATTCGAGAAGTGTTTGTGCTTCATAATCTCAAACTCTTCATGCTCATATTTAACAATAAGATCAGATTCAAGATTTAGGAAATAGGTCTGTGAACAAATCGACTGCTCATGAAAGATGCCAAAATCTGATTGTGAAGTTTCTTTACCGCCTTTCAGTAATACAAGTACAGCATAAGAAAAGACGATAATTCGCTGTTGCGTTTTATTACTGAAGTAATCAAATTTCAGTTTTTGCACAAATGTTGTTTGCTGAATCATGAAGTTCTGTATTAACTTTTCATGATATTTAATATTAATATCATAGTATTTGTTATCTCATGTTGAATGTTTGCTTTTCCGCTAAGACAATGATGATGGCTCAGACATGCAGACCTCATCTACTGGAGAGACTGTTGCATCAACACCATTGCAGAATGTCCTTGTGAGTCTTTCCCCCAACTCGATGGATGTGGATGTCTTGGTTAGTAGACCTTTTGCAGTGGTTGACGAGATATCAGATGCATCACCAGCGGTAGAAGATGGTTTACAGCTTGGAGATCAAATTTTGAAATTTGGCAACGTCGAAGCAGGTGAAAATTTGCTCCATAGGTTGGCTTCCGAGGCCCAGTCGAGCATGGGTCAAACAATACCTGTTGTCATCATGAGGCAAGGCACAGTGATCAACTTAACTGTTACACCTAGGACTTGGCAAGGCAGAGGTTTACTGGGGTATGCTTCCTGTCCCAATTATTTTGTTATTTGTTATCTTGTGTGCTTATAG

Protein sequence

>MS.gene78192.t1

MVGTNVKAETRSLMDKRSALESEMNSIIARLSQFPGAPGLSGNLIDSEGFPRSDIDVPLVRAERRRLAELRNDYTDITDKINQNIHILHSSRLGNHKNSDNDDGSDMQTSSTGETVASTPLQNVLVSLSPNSMDVDVLVSRPFAVVDEISDASPAVEDGLQLGDQILKFGNVEAGENLLHRLASEAQSSMGQTIPVVIMRQGTVINLTVTPRTWQGRGLLGYASCPNYFVICYLVCL