AlfalfaGEDB | MS.gene78306

M. sativa cultivar XinJiangDaYe / MS.gene78306

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78306.t1	XP_013452956.1	85.4	508	74	0	2	509	1	508	1.90E-112	416.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78306.t1	Q84VY2	49.0	51	26	0	457	507	463	513	2.1e-06	55.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78306.t1	A0A072UB28	85.4	508	74	0	2	509	1	508	1.4e-112	416.4

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049850	MS.gene78306	0.806315	8.73E-50	-1.69E-46
MS.gene050959	MS.gene78306	0.803991	2.68E-49	-1.69E-46
MS.gene051720	MS.gene78306	0.807838	4.16E-50	-1.69E-46
MS.gene052133	MS.gene78306	0.817867	2.64E-52	-1.69E-46
MS.gene052665	MS.gene78306	0.813087	3.06E-51	-1.69E-46
MS.gene052896	MS.gene78306	0.809061	2.28E-50	-1.69E-46
MS.gene055465	MS.gene78306	0.844392	7.78E-59	-1.69E-46
MS.gene055466	MS.gene78306	0.837216	5.93E-57	-1.69E-46
MS.gene055468	MS.gene78306	0.832336	1.00E-55	-1.69E-46
MS.gene055469	MS.gene78306	0.820745	5.83E-53	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78306.t1	MTR_6g082800	92.913	508	36	0	2	509	1	508	0.0	918
MS.gene78306.t1	MTR_8g032900	45.665	519	223	12	2	501	1	479	3.10e-109	333
MS.gene78306.t1	MTR_8g032930	44.007	559	208	15	2	509	1	505	7.51e-107	328
MS.gene78306.t1	MTR_3g093770	43.659	552	206	10	24	498	2	525	1.63e-106	328
MS.gene78306.t1	MTR_3g093750	45.842	469	195	11	2	448	1	432	2.80e-95	299
MS.gene78306.t1	MTR_5g075490	31.923	520	314	9	3	509	112	604	1.57e-59	207
MS.gene78306.t1	MTR_3g467610	27.043	514	279	11	21	509	94	536	8.46e-34	134
MS.gene78306.t1	MTR_3g093720	57.432	148	49	3	119	252	49	196	6.84e-33	127
MS.gene78306.t1	MTR_7g022780	36.726	226	114	3	233	458	3	199	1.32e-24	102

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78306.t1	AT5G05180	34.448	299	157	5	237	507	115	402	4.03e-40	150
MS.gene78306.t1	AT5G05180	34.448	299	157	5	237	507	145	432	4.11e-40	150
MS.gene78306.t1	AT5G05180	44.560	193	79	2	332	500	209	397	1.33e-37	143
MS.gene78306.t1	AT5G58320	25.954	524	287	13	9	509	113	558	9.70e-22	99.0
MS.gene78306.t1	AT5G58320	25.954	524	287	13	9	509	113	558	9.70e-22	99.0
MS.gene78306.t1	AT2G30500	28.070	399	206	13	118	508	189	514	8.28e-21	95.9
MS.gene78306.t1	AT2G30500	28.070	399	206	13	118	508	189	514	8.28e-21	95.9
MS.gene78306.t1	AT2G30500	28.070	399	206	13	118	508	188	513	1.12e-20	95.5
MS.gene78306.t1	AT3G10880	55.435	92	40	1	397	487	188	279	1.78e-18	86.3
MS.gene78306.t1	AT3G10880	55.435	92	40	1	397	487	188	279	1.78e-18	86.3

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Find 92 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGTGACTAAATTAGAA+TGG	0.541639	6.4:-56086926	MS.gene78306:CDS
AAAGACAAAACCAGTTTGTT+GGG	0.390775	6.4:-56087340	MS.gene78306:CDS
AAATTTGCAAGTTGGAAGAC+AGG	0.450922	6.4:-56086881	MS.gene78306:CDS
AAATTTGGTGGAACAATTGG+AGG	0.473827	6.4:-56087063	MS.gene78306:CDS
AACACAATTGACCTCAACAC+TGG	0.559176	6.4:-56086718	MS.gene78306:CDS
AACCAGTTTGTTGGGGAGGG+AGG	0.556196	6.4:-56087332	MS.gene78306:CDS
AAGACAAAACCAGTTTGTTG+GGG	0.607800	6.4:-56087339	MS.gene78306:CDS
AAGAGAAGAGGGAGGCAATT+CGG	0.438047	6.4:-56086251	MS.gene78306:CDS
AAGCAGGGATTGCAGAAGTC+TGG	0.482361	6.4:-56087581	MS.gene78306:CDS
AAGTCCAAATTGACGGGTTG+AGG	0.461874	6.4:+56087557	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGGAGTAAATAAGTACTTG+AGG	-	chr6.4:56086362-56086381	MS.gene78306:CDS	30.0%
AAGTCTGTAATTCATTACCA+TGG	-	chr6.4:56086964-56086983	MS.gene78306:CDS	30.0%
AGTAGAAGAACTTGAAAAAG+AGG	-	chr6.4:56088085-56088104	MS.gene78306:intron	30.0%
ATGTATCATGAACAATAGAC+TGG	+	chr6.4:56086580-56086599	None:intergenic	30.0%
CAAGATTAAGAAAGGAACTT+GGG	-	chr6.4:56087394-56087413	MS.gene78306:CDS	30.0%
CTTCATCATTTGATATCTTG+AGG	+	chr6.4:56087371-56087390	None:intergenic	30.0%
GAATCAAAATGAGAAAAGTG+AGG	-	chr6.4:56087194-56087213	MS.gene78306:CDS	30.0%
GATGAACTTGAGCATAAAAA+GGG	-	chr6.4:56087258-56087277	MS.gene78306:CDS	30.0%
GTTGTATAAATTATGTCTCC+TGG	+	chr6.4:56086318-56086337	None:intergenic	30.0%
TCATCAGAGAAGTAATGATT+TGG	-	chr6.4:56087836-56087855	MS.gene78306:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6.4	gene	56086155	56088262	56086155	ID=MS.gene78306
chr6.4	mRNA	56086155	56088262	56086155	ID=MS.gene78306.t1;Parent=MS.gene78306
chr6.4	exon	56088260	56088262	56088260	ID=MS.gene78306.t1.exon1;Parent=MS.gene78306.t1
chr6.4	CDS	56088260	56088262	56088260	ID=cds.MS.gene78306.t1;Parent=MS.gene78306.t1
chr6.4	exon	56086155	56087681	56086155	ID=MS.gene78306.t1.exon2;Parent=MS.gene78306.t1
chr6.4	CDS	56086155	56087681	56086155	ID=cds.MS.gene78306.t1;Parent=MS.gene78306.t1

Gene Sequence

>MS.gene78306

ATGGTATAGTCATTGTAAAATACCTATGTCAGTTGCAGACACTTACTCTCATAGGGCTAATATCAATAATAATTTATAAGTCGGAACCTTATTTTGCTTTGACAAATGAGTTGGGGTGCGTCTTTTATGCTTATTTGTTCAATCAAAAGATGCAATCTTTCCAGGAGACATAATTTATACAACATAATTAAAGTGTCATCTGTTTCAAAGGAGTAAATAAGTACTTGAGGTACAATTTTTTGCGTTGAGAGATTTAAAGTGTCTATGCTGGATGTTTTACTTTTGATATCTATTTTGATTGTAGGATTTTTAAACAGTTTTAGTGTTATGTTACTACTCTAGATTTGCTTCGCATCTCAAGAGAAGAGAATGATGCGAAAAAATGTGACCACTGTCACCATCTTACTGAATGGCTCTGCGATCCAGTCTATTGTTCATGATACATTTATTCATTTGGGTTCATTGAATTTTGCCTTGTTGATTATTTGATGTTTGGAATGGAAATGCTGTTCCTTTTAGGATTTTTCCCCCCTGTAATGCAGAGTACTTATCATATACTTTTTTTATCCTCACAATCTCAGATGGAGGGCACTGGTGATTTTGGCGAAGGTCTTGACAAAGAAAGTTCATTGGTGACTCCATATTATACTCCATACCAGAAGCAGGGATTGCAGAAGTCTGGTCCTCAACCCGTCAATTTGGACTTCTCTCCTAGCTCAGGTGGTGTTAGCTCTGCCGCTTCTCAAAAGGAGGGTTCTGAATCAAGTTCTTCATCATCCTCCTCTGATTCTGAAACTGACTCTTGTATCAAGTCTGTAATTCATTACCATGGCACGGCGGTGAATACTGACGATGAAGGAACCACTCAAGGTAAGCTGAAAGAAGACCTTGCTGATACCGAAAGACAAAACCAGTTTGTTGGGGAGGGAGGAAATAATAGTTATGATGAATTGCTGAAGAAGTTCATTAAAATTGAGGAAGAGCTACGAGTTTCCAATTTGAAACTTAAGCTTTCAGAAGAAGAGATTATCAAGTTGAAGAATCAAAATGAGAAAAGTGAGGGTCAGCTTGATAGTGTGCAGAAAGAATTAGCACTGAATAAGGATGAACTTGAGCATAAAAAGGGACAGGTGCTGGAATTGCAAAAGCAAAAAGCTGAGTTGGAGACTCATGTTCCAAATTTGGTGGAACAATTGGAGGTGGCTAATGAACACCTCAAGATATCAAATGATGAAGTTGCAAGATTAAGAAAGGAACTTGGGAGTAGTTCAGCTGAGACTCGTCAATTGCAAGACCAGCTTGAAGTGGCACAGGAAAATGTGACTAAATTAGAATGGCAGCTTGATTCAGGCAGAAAGCAAATTTGCAAGTTGGAAGACAGGATTACATGGTTTAAAACTAATGAAACTAACCTTGAAATTGAGGTGCAAAAACTGAAGGATCAAATGCATGATGTGCAGGCACAGTTCTCTTTTGAGAAAGATCAATTGCATTCTGATATTGCGAGCTTATCAGAAGTAAAAACACAATTGACCTCAACACTGGAGGAATGGGAGTCTAGAAGCCATTTATTAGCAAACAAGTTAAGGCGATGTGAAGCTGAAAATTTGGAGCACGAAGAGCTGTATGCTACACAACAAATGGTTATGCAAGGCGAAATCAGTTCTTTAAAGGAAGAACTTCATCAGAGAAGTAATGATTTGGAAGCTGTGAATAAGGATTTTGACAGGCATAAACAGAAATTTGACATGCTCATGACTGAAAAAGATGAAGTTAATGCCACGATTGATAAACTTAAGGCTGAAATAAGCTTTCGAGACGATCAAATAGAAAATATGGACCGAGAGCTTATCCAGCTTCGCGCACAAGAGAGTGAGCTGATCTCAAGATCTGAAACTAGACTGAATTTAGTAAATGAGTTGAAACTGAAAGTAGAAGAACTTGAAAAAGAGGTGACTAGACAGAATATTGTGATCTCCGATCGGGATGAAGAGAAGAGGGAGGCAATTCGGCAACTGTGTTTCTCACTTGATCACTACAAGAGTGGATATAAAGAAATTCTTCAAGCTTTTACTGGCCATAGGAGTCATACCGTTATAGCCTCCTAA

Protein sequence

>MS.gene78306.t1

MMEGTGDFGEGLDKESSLVTPYYTPYQKQGLQKSGPQPVNLDFSPSSGGVSSAASQKEGSESSSSSSSSDSETDSCIKSVIHYHGTAVNTDDEGTTQGKLKEDLADTERQNQFVGEGGNNSYDELLKKFIKIEEELRVSNLKLKLSEEEIIKLKNQNEKSEGQLDSVQKELALNKDELEHKKGQVLELQKQKAELETHVPNLVEQLEVANEHLKISNDEVARLRKELGSSSAETRQLQDQLEVAQENVTKLEWQLDSGRKQICKLEDRITWFKTNETNLEIEVQKLKDQMHDVQAQFSFEKDQLHSDIASLSEVKTQLTSTLEEWESRSHLLANKLRRCEAENLEHEELYATQQMVMQGEISSLKEELHQRSNDLEAVNKDFDRHKQKFDMLMTEKDEVNATIDKLKAEISFRDDQIENMDRELIQLRAQESELISRSETRLNLVNELKLKVEELEKEVTRQNIVISDRDEEKREAIRQLCFSLDHYKSGYKEILQAFTGHRSHTVIAS