Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78792.t1 | XP_003614714.1 | 82.2 | 202 | 30 | 1 | 1 | 196 | 1 | 202 | 7.60E-80 | 307.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78792.t1 | Q9FX64 | 69.0 | 71 | 22 | 0 | 225 | 295 | 149 | 219 | 1.6e-22 | 108.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78792.t1 | G7JXB6 | 82.2 | 202 | 30 | 1 | 1 | 196 | 1 | 202 | 5.5e-80 | 307.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052867 | MS.gene78792 | 0.801706 | 7.93E-49 | -1.69E-46 |
| MS.gene053974 | MS.gene78792 | 0.934127 | 6.53E-96 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene64804 | MS.gene78792 | PPI |
| MS.gene78792 | MS.gene035486 | PPI |
| MS.gene78792 | MS.gene67483 | PPI |
| MS.gene78792 | MS.gene93333 | PPI |
| MS.gene78792 | MS.gene040757 | PPI |
| MS.gene59503 | MS.gene78792 | PPI |
| MS.gene78792 | MS.gene073734 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78792.t1 | MTR_5g058150 | 81.304 | 230 | 30 | 2 | 1 | 224 | 1 | 223 | 3.92e-119 | 342 |
| MS.gene78792.t1 | MTR_6g075250 | 62.613 | 222 | 76 | 3 | 1 | 216 | 1 | 221 | 1.18e-83 | 252 |
| MS.gene78792.t1 | MTR_5g055150 | 95.556 | 90 | 4 | 0 | 206 | 295 | 130 | 219 | 4.81e-57 | 184 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78792.t1 | AT1G12650 | 54.545 | 154 | 67 | 2 | 46 | 196 | 48 | 201 | 5.79e-42 | 145 |
| MS.gene78792.t1 | AT1G12650 | 54.545 | 154 | 67 | 2 | 46 | 196 | 48 | 201 | 5.79e-42 | 145 |
| MS.gene78792.t1 | AT1G12650 | 54.545 | 154 | 67 | 2 | 46 | 196 | 48 | 201 | 5.79e-42 | 145 |
| MS.gene78792.t1 | AT1G12650 | 54.545 | 154 | 67 | 2 | 46 | 196 | 48 | 201 | 5.79e-42 | 145 |
| MS.gene78792.t1 | AT1G13330 | 67.416 | 89 | 29 | 0 | 207 | 295 | 131 | 219 | 2.25e-37 | 133 |
Find 64 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAGGATCCCAAAGAATTTA+AGG | 0.146216 | 5.1:+40053903 | MS.gene78792:CDS |
| CTTACATTTCTTGAGATAAA+AGG | 0.188990 | 5.1:-40052658 | None:intergenic |
| AAAAGAAACCTTAAATTCTT+TGG | 0.192821 | 5.1:-40053911 | None:intergenic |
| AAAGAATGTTCAGGGATTTA+TGG | 0.204783 | 5.1:+40053857 | MS.gene78792:CDS |
| ACAATTAATCTGACCTTCTT+AGG | 0.222334 | 5.1:-40050661 | None:intergenic |
| GAATATTGAGACACAGATTT+TGG | 0.225799 | 5.1:+40052587 | MS.gene78792:CDS |
| TCAACTGCCGCGCGTTCTTC+TGG | 0.252324 | 5.1:-40053793 | None:intergenic |
| TGGGTTTCATCGGTTGAATT+TGG | 0.277771 | 5.1:-40043102 | None:intergenic |
| CATCTATAAAATTCTACTTC+AGG | 0.308517 | 5.1:+40053714 | MS.gene78792:CDS |
| AAAGAAACCTTAAATTCTTT+GGG | 0.320146 | 5.1:-40053910 | None:intergenic |
| TGGCATAACAGGCTCTTAAA+AGG | 0.329338 | 5.1:+40052383 | MS.gene78792:intron |
| GGGTTTCATCGGTTGAATTT+GGG | 0.335327 | 5.1:-40043101 | None:intergenic |
| AAGAATGTTCAGGGATTTAT+GGG | 0.335669 | 5.1:+40053858 | MS.gene78792:CDS |
| AGAAGTTCAAAAGGGCTAAT+AGG | 0.344627 | 5.1:+40049619 | MS.gene78792:CDS |
| TTAAATTCTTTGGGATCCTT+AGG | 0.363696 | 5.1:-40053901 | None:intergenic |
| TGAACATTGCAGGAGGATCT+TGG | 0.381760 | 5.1:+40054002 | MS.gene78792:intron |
| AATGATTACCTTGTGGTATC+AGG | 0.391887 | 5.1:-40054073 | None:intergenic |
| GGAAGCGTAAAAGAATGTTC+AGG | 0.398097 | 5.1:+40053848 | MS.gene78792:CDS |
| TTGAACTTCTTATCTTCTGC+AGG | 0.399522 | 5.1:-40049606 | None:intergenic |
| CCACGATTTGAGTCTCTTTG+CGG | 0.402540 | 5.1:+40051109 | MS.gene78792:CDS |
| TCTACTTCAGGTTCAAGAAC+TGG | 0.406886 | 5.1:+40053726 | MS.gene78792:CDS |
| TGCGGTACATTTGACAATGA+TGG | 0.407412 | 5.1:+40051127 | MS.gene78792:CDS |
| GAACATTGCAGGAGGATCTT+GGG | 0.433317 | 5.1:+40054003 | MS.gene78792:intron |
| TTCTTGCTACTAGCTTCCAT+TGG | 0.435250 | 5.1:-40050604 | None:intergenic |
| TCAAAAGGGCTAATAGGAAT+AGG | 0.438404 | 5.1:+40049625 | MS.gene78792:CDS |
| TCTATTTCACTTCTACGCTC+TGG | 0.442608 | 5.1:-40052427 | None:intergenic |
| TTACATTTCTTGAGATAAAA+GGG | 0.446015 | 5.1:-40052657 | None:intergenic |
| CTCCTCGTAGTGGGTTTCAT+CGG | 0.452674 | 5.1:-40043112 | None:intergenic |
| CAGAAGATAAGAAGTTCAAA+AGG | 0.452716 | 5.1:+40049610 | MS.gene78792:CDS |
| GCGGTACATTTGACAATGAT+GGG | 0.485185 | 5.1:+40051128 | MS.gene78792:CDS |
| TGCAGAAAGCTCGATCCAAT+GGG | 0.499915 | 5.1:+40049562 | MS.gene78792:CDS |
| TGATCTTCCTGCTGAAAGAC+AGG | 0.502469 | 5.1:+40052284 | MS.gene78792:CDS |
| TTGCAGAAAGCTCGATCCAA+TGG | 0.504502 | 5.1:+40049561 | MS.gene78792:CDS |
| ACGTCTCTAAAAGCAGGAAC+TGG | 0.506037 | 5.1:-40050628 | None:intergenic |
| TTACCTACCTGTCTTTCAGC+AGG | 0.507162 | 5.1:-40052291 | None:intergenic |
| GAGAGAGAAGCAGCAAAGCA+AGG | 0.511269 | 5.1:+40052627 | MS.gene78792:CDS |
| AGTTTCATTCTCCTCGTAGT+GGG | 0.519416 | 5.1:-40043121 | None:intergenic |
| CAGTTTCATTCTCCTCGTAG+TGG | 0.524074 | 5.1:-40043122 | None:intergenic |
| GTTACGTGGAGGTGTTACTT+TGG | 0.529557 | 5.1:+40053765 | MS.gene78792:CDS |
| GGCAGTTGAGAGTACGTTGT+CGG | 0.546754 | 5.1:+40053807 | MS.gene78792:CDS |
| TACTTACATCTCTGATGAGA+AGG | 0.548825 | 5.1:-40043163 | None:intergenic |
| CGTCGTTCAAGCTCCTAAGA+AGG | 0.569172 | 5.1:+40050648 | MS.gene78792:CDS |
| AGAAGATAAGAAGTTCAAAA+GGG | 0.570488 | 5.1:+40049611 | MS.gene78792:CDS |
| GATACCACAAGGTAATCATT+CGG | 0.580909 | 5.1:+40054076 | MS.gene78792:CDS |
| GTGGAGGTGTTACTTTGGTG+AGG | 0.581557 | 5.1:+40053770 | MS.gene78792:CDS |
| GAAGCGTAAAAGAATGTTCA+GGG | 0.583026 | 5.1:+40053849 | MS.gene78792:CDS |
| TGAACGACGTCTCTAAAAGC+AGG | 0.592618 | 5.1:-40050634 | None:intergenic |
| TTAGAATATGATGAAGATGT+TGG | 0.594551 | 5.1:+40054026 | MS.gene78792:CDS |
| AAATAGAAGAGTACATAGCA+TGG | 0.598427 | 5.1:+40052443 | MS.gene78792:CDS |
| CCTCACTGAGAACTCACCTA+AGG | 0.600047 | 5.1:+40053885 | MS.gene78792:CDS |
| AATAGAAGAGTACATAGCAT+GGG | 0.631081 | 5.1:+40052444 | MS.gene78792:CDS |
| CTTAGGTGAGTTCTCAGTGA+GGG | 0.632171 | 5.1:-40053884 | None:intergenic |
| CCGCAAAGAGACTCAAATCG+TGG | 0.650639 | 5.1:-40051109 | None:intergenic |
| CGGAGAAAATAAGTCAGTGG+AGG | 0.655882 | 5.1:+40053827 | MS.gene78792:CDS |
| TGTCGGAGAAAATAAGTCAG+TGG | 0.667783 | 5.1:+40053824 | MS.gene78792:CDS |
| TGCAGTGACCTGATACCACA+AGG | 0.683122 | 5.1:+40054065 | MS.gene78792:CDS |
| GGTGAGGCCAGAAGAACGCG+CGG | 0.684052 | 5.1:+40053786 | MS.gene78792:CDS |
| TTTCTCAAAATGAACAAACA+CGG | 0.687082 | 5.1:+40043069 | None:intergenic |
| TCAACCGAATGATTACCTTG+TGG | 0.690079 | 5.1:-40054080 | None:intergenic |
| ATCAAGTTGAACAAGTTACG+TGG | 0.698323 | 5.1:+40053751 | MS.gene78792:CDS |
| AACCGATGAAACCCACTACG+AGG | 0.717095 | 5.1:+40043110 | MS.gene78792:CDS |
| GCAGAAAGCTCGATCCAATG+GGG | 0.727256 | 5.1:+40049563 | MS.gene78792:CDS |
| AAGTTGAACAAGTTACGTGG+AGG | 0.731034 | 5.1:+40053754 | MS.gene78792:CDS |
| CCTTAGGTGAGTTCTCAGTG+AGG | 0.748594 | 5.1:-40053885 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 40043078 | 40054124 | 40043078 | ID=MS.gene78792 |
| chr5.1 | mRNA | 40043078 | 40054124 | 40043078 | ID=MS.gene78792.t1;Parent=MS.gene78792 |
| chr5.1 | exon | 40043078 | 40043178 | 40043078 | ID=MS.gene78792.t1.exon1;Parent=MS.gene78792.t1 |
| chr5.1 | CDS | 40043078 | 40043178 | 40043078 | ID=cds.MS.gene78792.t1;Parent=MS.gene78792.t1 |
| chr5.1 | exon | 40049527 | 40049646 | 40049527 | ID=MS.gene78792.t1.exon2;Parent=MS.gene78792.t1 |
| chr5.1 | CDS | 40049527 | 40049646 | 40049527 | ID=cds.MS.gene78792.t1;Parent=MS.gene78792.t1 |
| chr5.1 | exon | 40050603 | 40050669 | 40050603 | ID=MS.gene78792.t1.exon3;Parent=MS.gene78792.t1 |
| chr5.1 | CDS | 40050603 | 40050669 | 40050603 | ID=cds.MS.gene78792.t1;Parent=MS.gene78792.t1 |
| chr5.1 | exon | 40051097 | 40051149 | 40051097 | ID=MS.gene78792.t1.exon4;Parent=MS.gene78792.t1 |
| chr5.1 | CDS | 40051097 | 40051149 | 40051097 | ID=cds.MS.gene78792.t1;Parent=MS.gene78792.t1 |
| chr5.1 | exon | 40052251 | 40052305 | 40052251 | ID=MS.gene78792.t1.exon5;Parent=MS.gene78792.t1 |
| chr5.1 | CDS | 40052251 | 40052305 | 40052251 | ID=cds.MS.gene78792.t1;Parent=MS.gene78792.t1 |
| chr5.1 | exon | 40052394 | 40052468 | 40052394 | ID=MS.gene78792.t1.exon6;Parent=MS.gene78792.t1 |
| chr5.1 | CDS | 40052394 | 40052468 | 40052394 | ID=cds.MS.gene78792.t1;Parent=MS.gene78792.t1 |
| chr5.1 | exon | 40052558 | 40052675 | 40052558 | ID=MS.gene78792.t1.exon7;Parent=MS.gene78792.t1 |
| chr5.1 | CDS | 40052558 | 40052675 | 40052558 | ID=cds.MS.gene78792.t1;Parent=MS.gene78792.t1 |
| chr5.1 | exon | 40053704 | 40053924 | 40053704 | ID=MS.gene78792.t1.exon8;Parent=MS.gene78792.t1 |
| chr5.1 | CDS | 40053704 | 40053924 | 40053704 | ID=cds.MS.gene78792.t1;Parent=MS.gene78792.t1 |
| chr5.1 | exon | 40054014 | 40054124 | 40054014 | ID=MS.gene78792.t1.exon9;Parent=MS.gene78792.t1 |
| chr5.1 | CDS | 40054014 | 40054124 | 40054014 | ID=cds.MS.gene78792.t1;Parent=MS.gene78792.t1 |
| Gene Sequence |
| Protein sequence |
