Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79105.t1 | XP_003601842.3 | 94.7 | 133 | 7 | 0 | 1 | 133 | 269 | 401 | 6.40E-68 | 266.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79105.t1 | A0A2P1GIW7 | 32.3 | 133 | 79 | 2 | 1 | 133 | 283 | 404 | 6.4e-16 | 85.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79105.t1 | G7J2I5 | 94.7 | 133 | 7 | 0 | 1 | 133 | 269 | 401 | 4.6e-68 | 266.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051201 | MS.gene79105 | 0.810843 | 9.42E-51 | -1.69E-46 |
| MS.gene056677 | MS.gene79105 | 0.80605 | 9.93E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79105.t1 | MTR_3g086020 | 94.737 | 133 | 7 | 0 | 1 | 133 | 269 | 401 | 3.59e-89 | 265 |
| MS.gene79105.t1 | MTR_8g079980 | 36.429 | 140 | 75 | 3 | 1 | 133 | 284 | 416 | 1.13e-21 | 89.4 |
| MS.gene79105.t1 | MTR_5g084850 | 36.842 | 133 | 78 | 3 | 1 | 129 | 276 | 406 | 1.11e-19 | 84.0 |
| MS.gene79105.t1 | MTR_5g084870 | 35.606 | 132 | 78 | 4 | 1 | 129 | 280 | 407 | 2.88e-19 | 82.8 |
| MS.gene79105.t1 | MTR_8g079970 | 35.766 | 137 | 82 | 4 | 1 | 133 | 283 | 417 | 4.57e-18 | 79.3 |
| MS.gene79105.t1 | MTR_5g084900 | 35.766 | 137 | 63 | 5 | 1 | 129 | 211 | 330 | 2.01e-17 | 77.0 |
| MS.gene79105.t1 | MTR_2g007880 | 30.405 | 148 | 83 | 4 | 1 | 133 | 294 | 436 | 8.21e-16 | 72.8 |
| MS.gene79105.t1 | MTR_8g079950 | 30.952 | 126 | 65 | 3 | 26 | 129 | 288 | 413 | 1.24e-14 | 69.3 |
| MS.gene79105.t1 | MTR_3g026950 | 31.783 | 129 | 77 | 4 | 3 | 121 | 300 | 427 | 4.17e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79105.t1 | AT1G24430 | 50.000 | 136 | 63 | 3 | 2 | 133 | 277 | 411 | 3.58e-41 | 142 |
| MS.gene79105.t1 | AT3G26040 | 31.159 | 138 | 89 | 3 | 1 | 133 | 279 | 415 | 1.83e-18 | 80.5 |
| MS.gene79105.t1 | AT1G24420 | 35.036 | 137 | 74 | 4 | 5 | 133 | 286 | 415 | 2.31e-15 | 71.6 |
| MS.gene79105.t1 | AT1G24420 | 35.036 | 137 | 74 | 4 | 5 | 133 | 286 | 415 | 2.31e-15 | 71.6 |
| MS.gene79105.t1 | AT5G47950 | 43.421 | 76 | 38 | 2 | 59 | 133 | 334 | 405 | 4.00e-14 | 68.2 |
| MS.gene79105.t1 | AT3G30280 | 30.216 | 139 | 88 | 3 | 3 | 133 | 285 | 422 | 8.02e-14 | 67.4 |
| MS.gene79105.t1 | AT5G23970 | 33.333 | 135 | 82 | 4 | 5 | 133 | 275 | 407 | 2.77e-13 | 65.9 |
| MS.gene79105.t1 | AT4G15390 | 44.828 | 58 | 31 | 1 | 77 | 133 | 368 | 425 | 1.18e-12 | 63.9 |
| MS.gene79105.t1 | AT4G15390 | 44.828 | 58 | 31 | 1 | 77 | 133 | 368 | 425 | 1.18e-12 | 63.9 |
| MS.gene79105.t1 | AT4G15400 | 33.010 | 103 | 63 | 3 | 34 | 133 | 315 | 414 | 4.86e-12 | 62.0 |
Find 0 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATTCTTAAAAGTCAAAGC+AGG | - | 22522:15241-15260 | MS.gene79105:intergenic | 25.0% |
| ! | TGATAAAGAATATGTGAAGA+AGG | + | 22522:15060-15079 | MS.gene79105:CDS | 25.0% |
| !! | TTAATTTTACCAGTTTGTGT+AGG | + | 22522:15161-15180 | MS.gene79105:CDS | 25.0% |
| !!! | TGCTTTGACTTTTAAGAATT+TGG | + | 22522:15240-15259 | MS.gene79105:CDS | 25.0% |
| ! | AGAGCATTTGGAATTTCTTA+AGG | + | 22522:15096-15115 | MS.gene79105:CDS | 30.0% |
| !! | TAAGGAAAGTTTTGATAGAG+TGG | + | 22522:15114-15133 | MS.gene79105:CDS | 30.0% |
| ATAACGATTCCGAAGATGAA+TGG | + | 22522:14983-15002 | MS.gene79105:CDS | 35.0% | |
| TAGTTACCAAACGATTCTGA+AGG | - | 22522:14950-14969 | MS.gene79105:intergenic | 35.0% | |
| TATGTGAAGAAGGTTCAAGA+AGG | + | 22522:15070-15089 | MS.gene79105:CDS | 35.0% | |
| ! | GGTTGTGTTTGTTGATTGTA+GGG | + | 22522:15261-15280 | MS.gene79105:CDS | 35.0% |
| ! | TGGTTGTGTTTGTTGATTGT+AGG | + | 22522:15260-15279 | MS.gene79105:CDS | 35.0% |
| ! | TGTTTGTTGATTGTAGGGAT+GGG | + | 22522:15266-15285 | MS.gene79105:CDS | 35.0% |
| ! | TTTTGATAGAGTGGTTGTGA+AGG | + | 22522:15123-15142 | MS.gene79105:CDS | 35.0% |
| !! | CCTTTGTATGATGCTGATTT+CGG | + | 22522:15187-15206 | MS.gene79105:CDS | 35.0% |
| !! | TCAAGAAGGTAAAGAGCATT+TGG | + | 22522:15084-15103 | MS.gene79105:CDS | 35.0% |
| CAAAGGAAACCTACACAAAC+TGG | - | 22522:15173-15192 | MS.gene79105:intergenic | 40.0% | |
| CCGAAATCAGCATCATACAA+AGG | - | 22522:15190-15209 | MS.gene79105:intergenic | 40.0% | |
| CTCAAGCTTCAAGCTAACAT+AGG | - | 22522:15305-15324 | MS.gene79105:intergenic | 40.0% | |
| ! | CCAAACGATTCTGAAGGTAA+CGG | - | 22522:14944-14963 | MS.gene79105:intergenic | 40.0% |
| ! | CTATGTTAGCTTGAAGCTTG+AGG | + | 22522:15303-15322 | MS.gene79105:CDS | 40.0% |
| ! | GTGTTTGTTGATTGTAGGGA+TGG | + | 22522:15265-15284 | MS.gene79105:CDS | 40.0% |
| ! | GTTTGTTGATTGTAGGGATG+GGG | + | 22522:15267-15286 | MS.gene79105:CDS | 40.0% |
| ! | TTTGTTGATTGTAGGGATGG+GGG | + | 22522:15268-15287 | MS.gene79105:CDS | 40.0% |
| !! | GTATGATGCTGATTTCGGTT+GGG | + | 22522:15192-15211 | MS.gene79105:CDS | 40.0% |
| !! | TATGATGCTGATTTCGGTTG+GGG | + | 22522:15193-15212 | MS.gene79105:CDS | 40.0% |
| !! | TGTATGATGCTGATTTCGGT+TGG | + | 22522:15191-15210 | MS.gene79105:CDS | 40.0% |
| ACGATTCCGAAGATGAATGG+CGG | + | 22522:14986-15005 | MS.gene79105:CDS | 45.0% | |
| AGTGGTTGTGAAGGAGGAAT+TGG | + | 22522:15132-15151 | MS.gene79105:CDS | 45.0% | |
| CCGTTACCTTCAGAATCGTT+TGG | + | 22522:14941-14960 | MS.gene79105:CDS | 45.0% | |
| TCTTCTCCGCCATTCATCTT+CGG | - | 22522:14995-15014 | MS.gene79105:intergenic | 45.0% | |
| TGATAGAGTGGTTGTGAAGG+AGG | + | 22522:15126-15145 | MS.gene79105:CDS | 45.0% | |
| ! | AACGATTCTGAAGGTAACGG+TGG | - | 22522:14941-14960 | MS.gene79105:intergenic | 45.0% |
| !! | AATGGCGGAGAAGAGTGTTA+CGG | + | 22522:15001-15020 | MS.gene79105:CDS | 45.0% |
| CGGTTGGGGCAAACCTATTT+GGG | + | 22522:15207-15226 | MS.gene79105:CDS | 50.0% | |
| GCAGGTGAACCAACCCAAAT+AGG | - | 22522:15223-15242 | MS.gene79105:intergenic | 50.0% | |
| TCGGTTGGGGCAAACCTATT+TGG | + | 22522:15206-15225 | MS.gene79105:CDS | 50.0% | |
| TGGGGCAAACCTATTTGGGT+TGG | + | 22522:15211-15230 | MS.gene79105:CDS | 50.0% | |
| !! | ATGGCGGAGAAGAGTGTTAC+GGG | + | 22522:15002-15021 | MS.gene79105:CDS | 50.0% |
| GTTGATTGTAGGGATGGGGG+TGG | + | 22522:15271-15290 | MS.gene79105:CDS | 55.0% | |
| TAGGGATGGGGGTGGTATTG+AGG | + | 22522:15279-15298 | MS.gene79105:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 22522 | gene | 14932 | 15330 | 14932 | ID=MS.gene79105 |
| 22522 | mRNA | 14932 | 15330 | 14932 | ID=MS.gene79105.t1;Parent=MS.gene79105 |
| 22522 | exon | 14932 | 15330 | 14932 | ID=MS.gene79105.t1.exon1;Parent=MS.gene79105.t1 |
| 22522 | CDS | 14932 | 15330 | 14932 | ID=cds.MS.gene79105.t1;Parent=MS.gene79105.t1 |
| Gene Sequence |
| Protein sequence |
