AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene79234

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79234.t1 MTR_3g087640 91.824 477 33 3 1 471 1 477 0.0 891
MS.gene79234.t1 MTR_2g089650 64.905 473 157 4 1 470 1 467 0.0 630
MS.gene79234.t1 MTR_2g089735 67.308 468 145 5 7 470 2 465 0.0 621
MS.gene79234.t1 MTR_2g089755 64.744 468 160 3 7 470 13 479 0.0 610
MS.gene79234.t1 MTR_3g435300 78.244 262 56 1 9 270 3 263 3.24e-149 426
MS.gene79234.t1 MTR_2g089765 35.861 488 262 15 10 467 17 483 3.77e-85 270
MS.gene79234.t1 MTR_7g014330 36.498 474 264 13 10 466 3 456 6.89e-83 263
MS.gene79234.t1 MTR_6g015850 37.740 469 251 15 11 467 19 458 5.09e-79 253
MS.gene79234.t1 MTR_7g014250 35.232 474 270 12 10 466 3 456 7.63e-79 253
MS.gene79234.t1 MTR_2g089670 35.010 477 268 16 10 466 23 477 2.83e-77 249
MS.gene79234.t1 MTR_7g015860 32.851 484 275 14 8 467 4 461 2.28e-75 244
MS.gene79234.t1 MTR_7g014230 34.100 478 274 13 7 465 2 457 2.06e-74 241
MS.gene79234.t1 MTR_6g015815 35.853 463 245 15 26 467 30 461 3.37e-73 238
MS.gene79234.t1 MTR_8g012750 34.016 488 274 14 10 467 4 473 8.04e-73 238
MS.gene79234.t1 MTR_7g014200 33.471 484 288 13 1 466 1 468 2.00e-72 236
MS.gene79234.t1 MTR_3g028210 32.489 474 287 12 10 467 9 465 4.31e-72 236
MS.gene79234.t1 MTR_6g015830 36.147 462 251 17 23 467 27 461 9.40e-70 229
MS.gene79234.t1 MTR_7g014610 33.742 489 270 15 10 466 9 475 4.71e-69 228
MS.gene79234.t1 MTR_7g014160 33.814 485 272 18 10 466 10 473 2.31e-68 226
MS.gene79234.t1 MTR_6g015320 30.258 466 284 12 17 467 16 455 7.25e-63 211
MS.gene79234.t1 MTR_6g015315 32.265 437 262 12 43 467 39 453 3.28e-62 209
MS.gene79234.t1 MTR_3g435290 80.992 121 23 0 350 470 1 121 9.15e-62 197
MS.gene79234.t1 MTR_4g032380 63.514 148 36 2 158 303 100 231 2.01e-60 197
MS.gene79234.t1 MTR_4g032380 61.644 73 25 1 9 81 32 101 1.59e-19 87.8
MS.gene79234.t1 MTR_7g013850 30.801 487 292 13 10 466 9 480 3.52e-55 191
MS.gene79234.t1 MTR_2g089665 30.112 445 184 14 10 440 3 334 3.12e-51 177
MS.gene79234.t1 MTR_7g014620 30.556 468 272 15 16 466 1 432 6.72e-51 179
MS.gene79234.t1 MTR_7g014360 30.958 407 225 11 75 466 21 386 8.42e-50 174
MS.gene79234.t1 MTR_2g067320 71.277 94 25 1 218 309 7 100 1.85e-40 142
MS.gene79234.t1 MTR_7g096620 59.504 121 42 3 218 334 7 124 1.66e-39 139
MS.gene79234.t1 MTR_2g104220 76.623 77 18 0 146 222 16 92 2.72e-39 137
MS.gene79234.t1 MTR_1g090750 67.010 97 30 1 215 309 4 100 4.08e-37 133
MS.gene79234.t1 MTR_1g036160 69.149 94 27 1 218 309 86 179 1.63e-36 133
MS.gene79234.t1 MTR_7g014670 37.824 193 112 6 281 468 72 261 4.02e-28 112
MS.gene79234.t1 MTR_8g096530 29.833 419 242 16 10 420 8 382 4.70e-28 116
MS.gene79234.t1 MTR_2g064495 25.862 464 287 20 13 461 7 428 1.09e-26 112
MS.gene79234.t1 MTR_7g014630 29.054 296 173 11 10 284 10 289 1.97e-26 110
MS.gene79234.t1 MTR_6g015310 25.542 415 212 15 41 444 5 333 7.65e-26 108
MS.gene79234.t1 MTR_7g014310 29.360 344 167 14 114 449 31 306 3.88e-25 105
MS.gene79234.t1 MTR_2g105330 25.431 464 289 20 13 461 7 428 7.81e-25 106
MS.gene79234.t1 MTR_4g007540 25.216 464 293 17 13 462 7 430 3.58e-24 104
MS.gene79234.t1 MTR_8g075610 25.747 435 268 17 41 462 25 417 4.61e-24 104
MS.gene79234.t1 MTR_3g098980 25.490 408 252 13 66 462 66 432 2.73e-23 102
MS.gene79234.t1 MTR_3g101900 25.400 437 273 14 30 450 30 429 8.58e-23 100
MS.gene79234.t1 MTR_4g022825 26.126 444 272 18 12 435 6 413 2.87e-21 96.3
MS.gene79234.t1 MTR_7g027175 24.880 418 260 15 13 421 20 392 1.09e-19 91.7
MS.gene79234.t1 MTR_7g014180 33.333 180 103 4 10 185 8 174 3.47e-19 85.5
MS.gene79234.t1 MTR_3g026950 24.638 414 247 19 62 444 50 429 6.12e-19 89.4
MS.gene79234.t1 MTR_4g022670 24.034 466 310 14 5 460 1 432 4.82e-18 86.7
MS.gene79234.t1 MTR_4g094842 26.914 431 269 19 7 423 2 400 6.56e-18 86.3
MS.gene79234.t1 MTR_7g085930 24.051 395 251 14 52 421 46 416 1.52e-17 85.5
MS.gene79234.t1 MTR_3g026310 24.449 454 294 16 20 456 14 435 2.47e-17 84.7
MS.gene79234.t1 MTR_4g015180 25.355 493 289 20 11 453 8 471 2.60e-17 84.7
MS.gene79234.t1 MTR_3g049150 23.636 385 230 16 63 420 52 399 1.26e-16 82.4
MS.gene79234.t1 MTR_4g094835 25.245 408 258 17 66 460 64 437 1.36e-16 82.4
MS.gene79234.t1 MTR_4g094828 24.295 461 277 20 23 456 22 437 1.37e-16 82.4
MS.gene79234.t1 MTR_4g127650 24.353 464 296 19 17 458 18 448 5.24e-16 80.5
MS.gene79234.t1 MTR_0190s0020 24.667 450 271 19 21 448 20 423 5.86e-16 80.1
MS.gene79234.t1 MTR_3g086020 23.579 475 269 22 8 460 4 406 8.44e-16 79.7
MS.gene79234.t1 MTR_3g070170 25.466 483 292 21 11 460 14 461 1.01e-15 79.7
MS.gene79234.t1 MTR_7g086000 23.585 424 277 10 17 420 12 408 2.09e-15 78.6
MS.gene79234.t1 MTR_2g104210 68.000 50 11 2 273 320 1 47 3.85e-15 70.5
MS.gene79234.t1 MTR_4g022290 25.240 416 237 21 30 424 35 397 4.76e-15 77.4
MS.gene79234.t1 MTR_4g097090 24.444 450 276 17 17 444 17 424 6.42e-14 73.9
MS.gene79234.t1 MTR_8g041770 26.923 286 171 11 154 430 144 400 8.19e-14 73.6
MS.gene79234.t1 MTR_7g014205 33.043 115 64 2 314 426 7 110 1.07e-13 68.2
MS.gene79234.t1 MTR_4g094832 27.273 275 170 12 156 424 133 383 3.41e-13 71.6
MS.gene79234.t1 MTR_8g079710 24.439 401 243 16 40 424 41 397 1.34e-12 69.7
MS.gene79234.t1 MTR_7g014210 36.893 103 62 2 327 426 24 126 1.49e-12 65.9
MS.gene79234.t1 MTR_4g055170 25.178 421 269 21 15 423 18 404 6.46e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79234.t1 AT1G03940 33.333 483 275 20 10 469 9 467 1.82e-71 234
MS.gene79234.t1 AT3G29670 31.224 474 279 15 11 466 5 449 5.52e-57 195
MS.gene79234.t1 AT3G29680 31.567 453 276 13 28 470 23 451 8.93e-57 195
MS.gene79234.t1 AT3G29635 31.858 452 275 13 28 468 27 456 9.62e-57 195
MS.gene79234.t1 AT5G61160 29.032 465 294 14 10 461 4 445 3.94e-53 185
MS.gene79234.t1 AT5G39090 28.326 466 299 13 12 467 8 448 4.00e-48 172
MS.gene79234.t1 AT5G39080 28.302 477 303 15 11 468 7 463 5.97e-48 171
MS.gene79234.t1 AT1G03495 33.427 356 203 15 9 351 8 342 1.37e-47 167
MS.gene79234.t1 AT3G29590 31.568 472 276 17 11 467 10 449 9.40e-47 168
MS.gene79234.t1 AT5G39050 29.979 477 296 15 10 467 11 468 6.71e-44 160
MS.gene79234.t1 AT5G48930 23.644 461 304 16 13 462 7 430 3.40e-21 96.3
MS.gene79234.t1 AT5G41040 24.408 422 259 16 13 421 21 395 3.71e-21 96.3
MS.gene79234.t1 AT5G41040 24.408 422 259 16 13 421 37 411 4.53e-21 95.9
MS.gene79234.t1 AT5G67150 24.790 476 293 20 8 460 4 437 2.02e-18 87.8
MS.gene79234.t1 AT5G63560 23.487 413 268 15 13 421 13 381 2.76e-18 87.4
MS.gene79234.t1 AT3G50270 23.849 478 280 20 6 450 3 429 3.19e-18 87.4
MS.gene79234.t1 AT5G57840 24.583 480 298 17 10 468 4 440 4.94e-18 86.7
MS.gene79234.t1 AT2G19070 23.884 448 288 16 17 443 12 427 5.44e-18 86.7
MS.gene79234.t1 AT2G39980 24.351 501 289 22 11 460 10 471 5.92e-18 86.7
MS.gene79234.t1 AT5G23940 25.652 460 271 20 30 455 25 447 9.03e-18 86.3
MS.gene79234.t1 AT5G42830 24.760 416 259 15 63 460 61 440 3.03e-17 84.3
MS.gene79234.t1 AT5G38130 23.851 457 275 17 7 444 22 424 3.81e-16 80.9
MS.gene79234.t1 AT5G01210 24.101 473 300 18 12 453 9 453 6.58e-15 77.4
MS.gene79234.t1 AT1G27620 26.190 420 251 16 13 421 13 384 6.62e-15 77.0
MS.gene79234.t1 AT5G07850 24.319 477 300 22 7 460 6 444 1.25e-14 76.3
MS.gene79234.t1 AT5G07860 24.576 472 293 19 7 456 6 436 3.53e-14 74.7
MS.gene79234.t1 AT3G29690 31.333 150 98 3 320 467 33 179 5.50e-14 70.5
MS.gene79234.t1 AT5G07870 23.542 480 308 20 7 460 6 452 7.77e-12 67.8
MS.gene79234.t1 AT4G31910 22.917 384 245 12 11 375 11 362 2.74e-11 65.9

Find 72 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATACCAGAGGGGTATTT+TGG 0.151654 3.1:-70454553 MS.gene79234:CDS
ACAAATTTAGATAGAAATTT+TGG 0.182553 3.1:+70454694 None:intergenic
TGAGATGGTGCATGCGTTTA+AGG 0.195519 3.1:-70454281 MS.gene79234:CDS
GGAGAAGGTGGACTTGAGTT+TGG 0.247995 3.1:-70454232 MS.gene79234:CDS
AATGTTTATGAGACCGATTT+TGG 0.269268 3.1:-70454325 MS.gene79234:CDS
GCAATCATTTCATGAAATCT+TGG 0.347391 3.1:-70454996 MS.gene79234:CDS
TGCACCATCTCAACTTTAAC+AGG 0.358365 3.1:+70454292 None:intergenic
CAGTCTTCTACATCTTTAAG+AGG 0.376958 3.1:+70454415 None:intergenic
AGTTTATGTTCTGCTCCTAT+AGG 0.377376 3.1:+70455540 None:intergenic
GTTGTTGTTGTTCTTGTTGC+AGG 0.384008 3.1:+70455495 None:intergenic
CAGCTACATCACCTCCTTGA+TGG 0.384620 3.1:+70454965 None:intergenic
CACATTCACCCTTCCATTGT+TGG 0.385846 3.1:-70455097 MS.gene79234:CDS
GAGAAGGTGGACTTGAGTTT+GGG 0.395274 3.1:-70454231 MS.gene79234:CDS
AATAAGCGTTTAGCGTAGAT+TGG 0.403428 3.1:+70455432 None:intergenic
AGTAAAACTATGTATATGTC+TGG 0.416871 3.1:-70454382 MS.gene79234:CDS
GTCATTAATATCTTCAATCC+TGG 0.423090 3.1:+70455131 None:intergenic
TAAGAAATGGCAAAACCTAT+AGG 0.429096 3.1:-70455555 None:intergenic
GAAAGAGATTGTTTGAGAGA+TGG 0.430195 3.1:+70455360 None:intergenic
ATAAGATTCTCAAGTCCTCT+TGG 0.434524 3.1:+70454898 None:intergenic
CATCATTTCTTCCCTCTCGC+TGG 0.448126 3.1:-70455327 MS.gene79234:CDS
GGTGGTGTTTGTTGGAGAGA+TGG 0.452853 3.1:+70455205 None:intergenic
AGGTCTTCCAAATCCAAAAT+CGG 0.460413 3.1:+70454312 None:intergenic
CCCAACCATGGTGTTTGCGT+TGG 0.463801 3.1:-70455054 MS.gene79234:CDS
TAAGATTCTCAAGTCCTCTT+GGG 0.479124 3.1:+70454899 None:intergenic
AAAGGTGACGGAGTCTTCTT+CGG 0.484341 3.1:+70455257 None:intergenic
GCCAACGCAAACACCATGGT+TGG 0.488721 3.1:+70455053 None:intergenic
TGGTTGGTTGAATGAGGAAA+AGG 0.489129 3.1:+70455402 None:intergenic
GAGATGGTGCATGCGTTTAA+GGG 0.496036 3.1:-70454280 MS.gene79234:CDS
TTTGGAAAAGAAGAAATTGA+GGG 0.502317 3.1:-70454772 MS.gene79234:CDS
AGATATTAATGACATTGAAA+AGG 0.509159 3.1:-70455121 MS.gene79234:CDS
TCTCTTGCTGAAAGTGAAGA+TGG 0.509760 3.1:-70454253 MS.gene79234:CDS
TGGGTTGGTGCTTAAAAGTG+AGG 0.512259 3.1:-70454212 MS.gene79234:CDS
CAATATTAACACACAAAACC+AGG 0.512389 3.1:-70455149 MS.gene79234:CDS
CCAACGCAAACACCATGGTT+GGG 0.516697 3.1:+70455054 None:intergenic
TGTTGTTAAGGTAATTGAAA+AGG 0.526288 3.1:-70454461 MS.gene79234:CDS
CTTGCAAAGCCAACAATGGA+AGG 0.529919 3.1:+70455088 None:intergenic
TCTTCGGTGCAGCGAATGAA+AGG 0.530046 3.1:+70455273 None:intergenic
GGAAATCAATTGTTTCTGCA+TGG 0.534473 3.1:-70454361 MS.gene79234:CDS
AAGTGCAACGATGAAGAGAA+AGG 0.537353 3.1:-70454512 MS.gene79234:CDS
CTCTTAAAGATGTAGAAGAC+TGG 0.550312 3.1:-70454414 MS.gene79234:CDS
GTCTCATAAACATTAAACTT+AGG 0.550716 3.1:+70454334 None:intergenic
AGGTGGACTTGAGTTTGGGT+TGG 0.563237 3.1:-70454227 MS.gene79234:CDS
CGATGATTCGATGATGGTAA+AGG 0.563360 3.1:+70455239 None:intergenic
GATGAAGAGAAAGGACATGA+AGG 0.568217 3.1:-70454503 MS.gene79234:CDS
GTTGCTCAAAGTCAAAGCAC+TGG 0.572117 3.1:-70454829 MS.gene79234:CDS
CAGTTTCCAAAATACCCCTC+TGG 0.574352 3.1:+70454547 None:intergenic
TTCGATGATGGTAAAGGTGA+CGG 0.584351 3.1:+70455245 None:intergenic
ATGAAGAGAAAGGACATGAA+GGG 0.596540 3.1:-70454502 MS.gene79234:CDS
GAAATGAGATTTCCAGCGAG+AGG 0.596654 3.1:+70455315 None:intergenic
CATTACATATTGTCACGTGA+TGG 0.601868 3.1:-70455031 MS.gene79234:CDS
ACATATTGTCACGTGATGGA+TGG 0.602963 3.1:-70455027 MS.gene79234:CDS
AAATGAGATTTCCAGCGAGA+GGG 0.605378 3.1:+70455316 None:intergenic
TTCTCTTCTCTCATTCAACA+AGG 0.605984 3.1:-70454178 MS.gene79234:CDS
CTTGGCTACTCAATACCAGA+GGG 0.607440 3.1:-70454562 MS.gene79234:CDS
GTCTCAATATTTCCCAACCA+TGG 0.616555 3.1:-70455066 MS.gene79234:CDS
ATCTTGCGATGATTCGATGA+TGG 0.618919 3.1:+70455233 None:intergenic
GAGACTTGCAAAGCCAACAA+TGG 0.623901 3.1:+70455084 None:intergenic
TGTGTGTTAATATTGGAACA+AGG 0.625336 3.1:+70455157 None:intergenic
TTGGAAAAGAAGAAATTGAG+GGG 0.626217 3.1:-70454771 MS.gene79234:CDS
GACCAGCAAAATGCAAGTCG+AGG 0.629102 3.1:+70455454 None:intergenic
GAAAGTGAAGATGGAGAAGG+TGG 0.637173 3.1:-70454244 MS.gene79234:CDS
AAGACCTGTTAAAGTTGAGA+TGG 0.641370 3.1:-70454296 MS.gene79234:CDS
TTGCAAAGCCAACAATGGAA+GGG 0.646182 3.1:+70455089 None:intergenic
TGTGATTGCAGAGATAGACT+TGG 0.654998 3.1:-70454580 MS.gene79234:CDS
AAAACTTCTCTATCAAAACA+AGG 0.666225 3.1:+70454928 None:intergenic
GCAATGCATAGAAATGATGA+CGG 0.667461 3.1:-70454634 MS.gene79234:CDS
TAATGCCAACGCAAACACCA+TGG 0.675041 3.1:+70455049 None:intergenic
TGCAAGTCGAGGAATGTGAG+AGG 0.687948 3.1:+70455465 None:intergenic
ACTTGGCTACTCAATACCAG+AGG 0.710749 3.1:-70454563 MS.gene79234:CDS
GCTGAAAGTGAAGATGGAGA+AGG 0.712274 3.1:-70454247 MS.gene79234:CDS
ACTTCTCTATCAAAACAAGG+TGG 0.730371 3.1:+70454931 None:intergenic
TTGGCTACTCAATACCAGAG+GGG 0.789894 3.1:-70454561 MS.gene79234:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACAAATTTAGATAGAAATTT+TGG + chr3.1:70455014-70455033 None:intergenic 15.0%
!!! ATTTTTTTACAAGAAAATCT+TGG - chr3.1:70454841-70454860 MS.gene79234:CDS 15.0%
!!! TTTAAGGTAATTATTGTTTT+TGG - chr3.1:70454915-70454934 MS.gene79234:CDS 15.0%
!! AGATATTAATGACATTGAAA+AGG - chr3.1:70454584-70454603 MS.gene79234:CDS 20.0%
!!! TATTTTCTTTGAGATTTTTC+AGG + chr3.1:70454524-70454543 None:intergenic 20.0%
! AAAACTTCTCTATCAAAACA+AGG + chr3.1:70454780-70454799 None:intergenic 25.0%
! AGTAAAACTATGTATATGTC+TGG - chr3.1:70455323-70455342 MS.gene79234:CDS 25.0%
! GTCTCATAAACATTAAACTT+AGG + chr3.1:70455374-70455393 None:intergenic 25.0%
! TGTTGTTAAGGTAATTGAAA+AGG - chr3.1:70455244-70455263 MS.gene79234:CDS 25.0%
! TTTGGAAAAGAAGAAATTGA+GGG - chr3.1:70454933-70454952 MS.gene79234:CDS 25.0%
!! AGAACATGAAGATTCTTTTA+AGG - chr3.1:70454899-70454918 MS.gene79234:CDS 25.0%
!! TTGTAACTAGTGCTTTTATT+TGG - chr3.1:70455033-70455052 MS.gene79234:CDS 25.0%
!!! TAGTTTTGTTGATGTTGTTA+AGG - chr3.1:70455232-70455251 MS.gene79234:CDS 25.0%
!!! TGTAACTAGTGCTTTTATTT+GGG - chr3.1:70455034-70455053 MS.gene79234:CDS 25.0%
!!! TTTTGATAGAGAAGTTTTGA+GGG - chr3.1:70454782-70454801 MS.gene79234:CDS 25.0%
!!! TTTTGGAAAAGAAGAAATTG+AGG - chr3.1:70454932-70454951 MS.gene79234:CDS 25.0%
AAATTGAGGGGATGAAAAAA+TGG - chr3.1:70454946-70454965 MS.gene79234:CDS 30.0%
AATTGAGGGGATGAAAAAAT+GGG - chr3.1:70454947-70454966 MS.gene79234:CDS 30.0%
CAATATTAACACACAAAACC+AGG - chr3.1:70454556-70454575 MS.gene79234:CDS 30.0%
GCAATCATTTCATGAAATCT+TGG - chr3.1:70454709-70454728 MS.gene79234:CDS 30.0%
GTCATTAATATCTTCAATCC+TGG + chr3.1:70454577-70454596 None:intergenic 30.0%
TGTGTGTTAATATTGGAACA+AGG + chr3.1:70454551-70454570 None:intergenic 30.0%
TTGGAAAAGAAGAAATTGAG+GGG - chr3.1:70454934-70454953 MS.gene79234:CDS 30.0%
!! AATGTTTATGAGACCGATTT+TGG - chr3.1:70455380-70455399 MS.gene79234:CDS 30.0%
!! GTTTTGATAGAGAAGTTTTG+AGG - chr3.1:70454781-70454800 MS.gene79234:CDS 30.0%
!!! CTGGTTTTGTGTGTTAATAT+TGG + chr3.1:70454558-70454577 None:intergenic 30.0%
!!! TTTCTTTGAGATTTTTCAGG+TGG + chr3.1:70454521-70454540 None:intergenic 30.0%
AAGACCTGTTAAAGTTGAGA+TGG - chr3.1:70455409-70455428 MS.gene79234:CDS 35.0%
AATAAGCGTTTAGCGTAGAT+TGG + chr3.1:70454276-70454295 None:intergenic 35.0%
ACTTCTCTATCAAAACAAGG+TGG + chr3.1:70454777-70454796 None:intergenic 35.0%
AGGTCTTCCAAATCCAAAAT+CGG + chr3.1:70455396-70455415 None:intergenic 35.0%
AGTTTATGTTCTGCTCCTAT+AGG + chr3.1:70454168-70454187 None:intergenic 35.0%
ATAAGATTCTCAAGTCCTCT+TGG + chr3.1:70454810-70454829 None:intergenic 35.0%
ATGAAGAGAAAGGACATGAA+GGG - chr3.1:70455203-70455222 MS.gene79234:CDS 35.0%
CAGTCTTCTACATCTTTAAG+AGG + chr3.1:70455293-70455312 None:intergenic 35.0%
CATTACATATTGTCACGTGA+TGG - chr3.1:70454674-70454693 MS.gene79234:CDS 35.0%
CTCTTAAAGATGTAGAAGAC+TGG - chr3.1:70455291-70455310 MS.gene79234:CDS 35.0%
GAAAGAGATTGTTTGAGAGA+TGG + chr3.1:70454348-70454367 None:intergenic 35.0%
GCAATGCATAGAAATGATGA+CGG - chr3.1:70455071-70455090 MS.gene79234:CDS 35.0%
TAAGATTCTCAAGTCCTCTT+GGG + chr3.1:70454809-70454828 None:intergenic 35.0%
TTCTCTTCTCTCATTCAACA+AGG - chr3.1:70455527-70455546 MS.gene79234:CDS 35.0%
! CAAAAATGGTTGGTTGAATG+AGG + chr3.1:70454312-70454331 None:intergenic 35.0%
!! AAAAATGGGTGCTGAATCAA+TGG - chr3.1:70454961-70454980 MS.gene79234:CDS 35.0%
!! GAAGTGTTGCTTTGCAAAAA+TGG + chr3.1:70454326-70454345 None:intergenic 35.0%
!! GGAAATCAATTGTTTCTGCA+TGG - chr3.1:70455344-70455363 MS.gene79234:CDS 35.0%
!! TATGAGACCGATTTTGGATT+TGG - chr3.1:70455386-70455405 MS.gene79234:CDS 35.0%
!! TCTTGGTCTTTTATCCATCA+AGG - chr3.1:70454726-70454745 MS.gene79234:CDS 35.0%
!! TGTTGCTTTGCAAAAATGGT+TGG + chr3.1:70454322-70454341 None:intergenic 35.0%
!!! ATTTTTCAGGTGGTGTTTGT+TGG + chr3.1:70454511-70454530 None:intergenic 35.0%
!!! TGCTTTTATTTGGGCTAGTA+TGG - chr3.1:70455043-70455062 MS.gene79234:CDS 35.0%
AAATGAGATTTCCAGCGAGA+GGG + chr3.1:70454392-70454411 None:intergenic 40.0%
AAGTGCAACGATGAAGAGAA+AGG - chr3.1:70455193-70455212 MS.gene79234:CDS 40.0%
ATCTTGCGATGATTCGATGA+TGG + chr3.1:70454475-70454494 None:intergenic 40.0%
GATGAAGAGAAAGGACATGA+AGG - chr3.1:70455202-70455221 MS.gene79234:CDS 40.0%
GTCTCAATATTTCCCAACCA+TGG - chr3.1:70454639-70454658 MS.gene79234:CDS 40.0%
TCTCTTGCTGAAAGTGAAGA+TGG - chr3.1:70455452-70455471 MS.gene79234:CDS 40.0%
TGCACCATCTCAACTTTAAC+AGG + chr3.1:70455416-70455435 None:intergenic 40.0%
TGTGATTGCAGAGATAGACT+TGG - chr3.1:70455125-70455144 MS.gene79234:CDS 40.0%
TTGCAAAGCCAACAATGGAA+GGG + chr3.1:70454619-70454638 None:intergenic 40.0%
! ACATATTGTCACGTGATGGA+TGG - chr3.1:70454678-70454697 MS.gene79234:CDS 40.0%
! ATGAAAGGTTTTTGTGGTGG+TGG + chr3.1:70454420-70454439 None:intergenic 40.0%
! CAGCGAATGAAAGGTTTTTG+TGG + chr3.1:70454426-70454445 None:intergenic 40.0%
! CGAATGAAAGGTTTTTGTGG+TGG + chr3.1:70454423-70454442 None:intergenic 40.0%
! CGATGATTCGATGATGGTAA+AGG + chr3.1:70454469-70454488 None:intergenic 40.0%
! GTTGTTGTTGTTCTTGTTGC+AGG + chr3.1:70454213-70454232 None:intergenic 40.0%
! TGGTCTTTTATCCATCAAGG+AGG - chr3.1:70454729-70454748 MS.gene79234:CDS 40.0%
! TGGTTGGTTGAATGAGGAAA+AGG + chr3.1:70454306-70454325 None:intergenic 40.0%
! TTCGATGATGGTAAAGGTGA+CGG + chr3.1:70454463-70454482 None:intergenic 40.0%
!!! TCAATACCAGAGGGGTATTT+TGG - chr3.1:70455152-70455171 MS.gene79234:CDS 40.0%
ACTTGGCTACTCAATACCAG+AGG - chr3.1:70455142-70455161 MS.gene79234:CDS 45.0%
CACATTCACCCTTCCATTGT+TGG - chr3.1:70454608-70454627 MS.gene79234:CDS 45.0%
CAGTTTCCAAAATACCCCTC+TGG + chr3.1:70455161-70455180 None:intergenic 45.0%
CTTGCAAAGCCAACAATGGA+AGG + chr3.1:70454620-70454639 None:intergenic 45.0%
CTTGGCTACTCAATACCAGA+GGG - chr3.1:70455143-70455162 MS.gene79234:CDS 45.0%
GAAAGTGAAGATGGAGAAGG+TGG - chr3.1:70455461-70455480 MS.gene79234:CDS 45.0%
GAAATGAGATTTCCAGCGAG+AGG + chr3.1:70454393-70454412 None:intergenic 45.0%
GAGACTTGCAAAGCCAACAA+TGG + chr3.1:70454624-70454643 None:intergenic 45.0%
GAGATGGTGCATGCGTTTAA+GGG - chr3.1:70455425-70455444 MS.gene79234:CDS 45.0%
GCTGAAAGTGAAGATGGAGA+AGG - chr3.1:70455458-70455477 MS.gene79234:CDS 45.0%
GTTGCTCAAAGTCAAAGCAC+TGG - chr3.1:70454876-70454895 MS.gene79234:CDS 45.0%
TAATGCCAACGCAAACACCA+TGG + chr3.1:70454659-70454678 None:intergenic 45.0%
TGAGATGGTGCATGCGTTTA+AGG - chr3.1:70455424-70455443 MS.gene79234:CDS 45.0%
TTGGCTACTCAATACCAGAG+GGG - chr3.1:70455144-70455163 MS.gene79234:CDS 45.0%
! GAGAAGGTGGACTTGAGTTT+GGG - chr3.1:70455474-70455493 MS.gene79234:CDS 45.0%
!! AAAGGTGACGGAGTCTTCTT+CGG + chr3.1:70454451-70454470 None:intergenic 45.0%
!! TGGGTTGGTGCTTAAAAGTG+AGG - chr3.1:70455493-70455512 MS.gene79234:CDS 45.0%
!! TTCCTCGACTTGCATTTTGC+TGG - chr3.1:70454249-70454268 MS.gene79234:CDS 45.0%
!!! AAAGGTTTTTGTGGTGGTGG+TGG + chr3.1:70454417-70454436 None:intergenic 45.0%
CAGCTACATCACCTCCTTGA+TGG + chr3.1:70454743-70454762 None:intergenic 50.0%
CATCATTTCTTCCCTCTCGC+TGG - chr3.1:70454378-70454397 MS.gene79234:CDS 50.0%
CCAACGCAAACACCATGGTT+GGG + chr3.1:70454654-70454673 None:intergenic 50.0%
GACCAGCAAAATGCAAGTCG+AGG + chr3.1:70454254-70454273 None:intergenic 50.0%
TGCAAGTCGAGGAATGTGAG+AGG + chr3.1:70454243-70454262 None:intergenic 50.0%
! AGGTGGACTTGAGTTTGGGT+TGG - chr3.1:70455478-70455497 MS.gene79234:CDS 50.0%
! GGAGAAGGTGGACTTGAGTT+TGG - chr3.1:70455473-70455492 MS.gene79234:CDS 50.0%
! GGTGGTGTTTGTTGGAGAGA+TGG + chr3.1:70454503-70454522 None:intergenic 50.0%
! TCTTCGGTGCAGCGAATGAA+AGG + chr3.1:70454435-70454454 None:intergenic 50.0%
!!! GAAGTTTTGAGGGACCCAAG+AGG - chr3.1:70454792-70454811 MS.gene79234:CDS 50.0%
CCCAACCATGGTGTTTGCGT+TGG - chr3.1:70454651-70454670 MS.gene79234:CDS 55.0%
GCCAACGCAAACACCATGGT+TGG + chr3.1:70454655-70454674 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 70454156 70455571 70454156 ID=MS.gene79234
chr3.1 mRNA 70454156 70455571 70454156 ID=MS.gene79234.t1;Parent=MS.gene79234
chr3.1 exon 70454156 70455571 70454156 ID=MS.gene79234.t1.exon1;Parent=MS.gene79234.t1
chr3.1 CDS 70454156 70455571 70454156 ID=cds.MS.gene79234.t1;Parent=MS.gene79234.t1
Gene Sequence

>MS.gene79234

ATGGCAAAACCTATAGGAGCAGAACATAAACTTATTGAGCAAACTCTTGTGTTTCCTGCAACAAGAACAACAACAACTTCTCTTCCTCTCACATTCCTCGACTTGCATTTTGCTGGTCCAATCTACGCTAAACGCTTATTCTTCTATCCTTTTCCTCATTCAACCAACCATTTTTGCAAAGCAACACTTCCATCTCTCAAACAATCTCTTTCTTGTGTTCTTCATCATTTCTTCCCTCTCGCTGGAAATCTCATTTCTCCACCACCACCACAAAAACCTTTCATTCGCTGCACCGAAGAAGACTCCGTCACCTTTACCATCATCGAATCATCGCAAGATTTCAACCATCTCTCCAACAAACACCACCTGAAAAATCTCAAAGAAAATAATAACCTTGTTCCAATATTAACACACAAAACCAGGATTGAAGATATTAATGACATTGAAAAGGACACATTCACCCTTCCATTGTTGGCTTTGCAAGTCTCAATATTTCCCAACCATGGTGTTTGCGTTGGCATTACATATTGTCACGTGATGGATGGTAACAGTTGCAATCATTTCATGAAATCTTGGTCTTTTATCCATCAAGGAGGTGATGTAGCTGAATTGAAGTCACCACCTTGTTTTGATAGAGAAGTTTTGAGGGACCCAAGAGGACTTGAGAATCTTATTTTAAGAGATTATTTTTTTACAAGAAAATCTTGGAAGTTTAGACTCGTTGCTCAAAGTCAAAGCACTGGAGAACATGAAGATTCTTTTAAGGTAATTATTGTTTTTGGAAAAGAAGAAATTGAGGGGATGAAAAAATGGGTGCTGAATCAATGGAAGAAAAATGATAATGAAATCAACGTGCCAAAATTTCTATCTAAATTTGTTGTAACTAGTGCTTTTATTTGGGCTAGTATGGTTAAAGCAATGCATAGAAATGATGACGGTGATGAAAAAGATGAGTACTTTTGTTTCACATGTGATTGCAGAGATAGACTTGGCTACTCAATACCAGAGGGGTATTTTGGAAACTGTGTAGTGTCTAAAAGTGCAACGATGAAGAGAAAGGACATGAAGGGAAAAAATAGTTTTGTTGATGTTGTTAAGGTAATTGAAAAGGCTGTTAATGAGATAAAAAATGAACCTCTTAAAGATGTAGAAGACTGGTTTGAATTAAGTAAAACTATGTATATGTCTGGAAATCAATTGTTTCTGCATGGATCACCTAAGTTTAATGTTTATGAGACCGATTTTGGATTTGGAAGACCTGTTAAAGTTGAGATGGTGCATGCGTTTAAGGGTGTGTCTCTTGCTGAAAGTGAAGATGGAGAAGGTGGACTTGAGTTTGGGTTGGTGCTTAAAAGTGAGGAATTTGAATATTTCTCTTCTCTCATTCAACAAGGACTTGAAGTTTTTAAACATTAA
Protein sequence

>MS.gene79234.t1

MAKPIGAEHKLIEQTLVFPATRTTTTSLPLTFLDLHFAGPIYAKRLFFYPFPHSTNHFCKATLPSLKQSLSCVLHHFFPLAGNLISPPPPQKPFIRCTEEDSVTFTIIESSQDFNHLSNKHHLKNLKENNNLVPILTHKTRIEDINDIEKDTFTLPLLALQVSIFPNHGVCVGITYCHVMDGNSCNHFMKSWSFIHQGGDVAELKSPPCFDREVLRDPRGLENLILRDYFFTRKSWKFRLVAQSQSTGEHEDSFKVIIVFGKEEIEGMKKWVLNQWKKNDNEINVPKFLSKFVVTSAFIWASMVKAMHRNDDGDEKDEYFCFTCDCRDRLGYSIPEGYFGNCVVSKSATMKRKDMKGKNSFVDVVKVIEKAVNEIKNEPLKDVEDWFELSKTMYMSGNQLFLHGSPKFNVYETDFGFGRPVKVEMVHAFKGVSLAESEDGEGGLEFGLVLKSEEFEYFSSLIQQGLEVFKH