AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052225

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052225.t1 MTR_3g087640 92.243 477 31 3 1 471 1 477 0.0 895
MS.gene052225.t1 MTR_2g089650 65.328 473 155 4 1 470 1 467 0.0 635
MS.gene052225.t1 MTR_2g089735 67.735 468 143 5 7 470 2 465 0.0 626
MS.gene052225.t1 MTR_2g089755 64.744 468 160 3 7 470 13 479 0.0 609
MS.gene052225.t1 MTR_3g435300 78.626 262 55 1 9 270 3 263 6.58e-150 428
MS.gene052225.t1 MTR_2g089765 36.066 488 261 15 10 467 17 483 1.32e-85 271
MS.gene052225.t1 MTR_7g014330 36.709 474 263 13 10 466 3 456 4.35e-83 264
MS.gene052225.t1 MTR_6g015850 37.953 469 250 15 11 467 19 458 2.12e-79 254
MS.gene052225.t1 MTR_7g014250 35.368 475 268 13 10 466 3 456 3.18e-79 254
MS.gene052225.t1 MTR_2g089670 35.220 477 267 16 10 466 23 477 1.17e-77 250
MS.gene052225.t1 MTR_7g015860 33.058 484 274 14 8 467 4 461 9.44e-76 245
MS.gene052225.t1 MTR_7g014230 34.310 478 273 13 7 465 2 457 8.28e-75 242
MS.gene052225.t1 MTR_6g015815 36.069 463 244 15 26 467 30 461 1.49e-73 239
MS.gene052225.t1 MTR_8g012750 34.221 488 273 14 10 467 4 473 2.72e-73 239
MS.gene052225.t1 MTR_7g014200 33.678 484 287 13 1 466 1 468 7.62e-73 238
MS.gene052225.t1 MTR_3g028210 32.700 474 286 12 10 467 9 465 1.91e-72 236
MS.gene052225.t1 MTR_6g015830 36.364 462 250 17 23 467 27 461 4.25e-70 230
MS.gene052225.t1 MTR_7g014610 33.742 489 270 15 10 466 9 475 1.47e-68 226
MS.gene052225.t1 MTR_7g014160 33.814 485 272 18 10 466 10 473 6.36e-68 225
MS.gene052225.t1 MTR_3g435290 81.818 121 22 0 350 470 1 121 4.92e-63 200
MS.gene052225.t1 MTR_6g015320 30.472 466 283 12 17 467 16 455 7.10e-63 211
MS.gene052225.t1 MTR_6g015315 32.265 437 262 12 43 467 39 453 5.97e-62 209
MS.gene052225.t1 MTR_4g032380 63.514 148 36 2 158 303 100 231 2.01e-60 197
MS.gene052225.t1 MTR_4g032380 58.750 80 30 1 9 88 32 108 4.85e-20 89.4
MS.gene052225.t1 MTR_7g013850 30.801 487 292 13 10 466 9 480 6.05e-55 191
MS.gene052225.t1 MTR_2g089665 30.337 445 183 14 10 440 3 334 1.10e-51 178
MS.gene052225.t1 MTR_7g014620 30.556 468 272 15 16 466 1 432 1.36e-50 178
MS.gene052225.t1 MTR_7g014360 30.958 407 225 11 75 466 21 386 2.00e-49 174
MS.gene052225.t1 MTR_2g067320 71.277 94 25 1 218 309 7 100 1.79e-40 142
MS.gene052225.t1 MTR_7g096620 59.504 121 42 3 218 334 7 124 1.59e-39 139
MS.gene052225.t1 MTR_2g104220 76.623 77 18 0 146 222 16 92 2.89e-39 137
MS.gene052225.t1 MTR_1g090750 67.010 97 30 1 215 309 4 100 3.79e-37 133
MS.gene052225.t1 MTR_1g036160 69.149 94 27 1 218 309 86 179 1.56e-36 133
MS.gene052225.t1 MTR_8g096530 29.833 419 242 16 10 420 8 382 4.35e-28 116
MS.gene052225.t1 MTR_7g014670 37.824 193 112 6 281 468 72 261 1.02e-27 111
MS.gene052225.t1 MTR_7g014630 29.054 296 173 11 10 284 10 289 2.49e-26 110
MS.gene052225.t1 MTR_2g064495 25.647 464 288 20 13 461 7 428 4.73e-26 110
MS.gene052225.t1 MTR_6g015310 24.819 415 215 13 41 444 5 333 1.47e-25 107
MS.gene052225.t1 MTR_7g014310 29.360 344 167 14 114 449 31 306 1.04e-24 104
MS.gene052225.t1 MTR_8g075610 25.977 435 267 17 41 462 25 417 1.05e-24 106
MS.gene052225.t1 MTR_2g105330 25.216 464 290 20 13 461 7 428 2.91e-24 105
MS.gene052225.t1 MTR_3g098980 25.622 402 259 12 66 462 66 432 5.42e-24 104
MS.gene052225.t1 MTR_4g007540 25.161 465 292 19 13 462 7 430 1.44e-23 103
MS.gene052225.t1 MTR_3g101900 24.885 434 279 13 30 450 30 429 1.01e-22 100
MS.gene052225.t1 MTR_4g022825 25.328 458 293 17 12 456 6 427 8.13e-22 98.2
MS.gene052225.t1 MTR_4g022800 24.893 466 299 17 5 456 1 429 1.49e-21 97.4
MS.gene052225.t1 MTR_4g022720 24.944 449 285 17 20 456 14 422 9.11e-21 94.7
MS.gene052225.t1 MTR_7g014180 33.889 180 102 4 10 185 8 174 8.75e-20 87.0
MS.gene052225.t1 MTR_7g027175 24.880 418 260 15 13 421 20 392 1.13e-19 91.7
MS.gene052225.t1 MTR_4g022670 24.464 466 308 14 5 460 1 432 1.28e-19 91.3
MS.gene052225.t1 MTR_3g026950 24.086 465 283 21 11 444 4 429 4.10e-19 90.1
MS.gene052225.t1 MTR_7g085930 23.308 399 260 12 47 421 40 416 1.12e-17 85.9
MS.gene052225.t1 MTR_4g094828 24.512 461 276 20 23 456 22 437 1.29e-17 85.5
MS.gene052225.t1 MTR_4g094835 25.553 407 258 16 66 460 64 437 1.67e-17 85.1
MS.gene052225.t1 MTR_4g015180 25.709 494 286 21 11 453 8 471 2.67e-17 84.7
MS.gene052225.t1 MTR_3g049150 23.472 409 245 17 63 440 52 423 1.21e-16 82.4
MS.gene052225.t1 MTR_0190s0020 24.390 451 285 18 21 456 20 429 2.93e-16 81.3
MS.gene052225.t1 MTR_3g070170 25.208 480 297 19 11 460 14 461 3.08e-16 81.3
MS.gene052225.t1 MTR_3g088420 25.676 444 266 19 30 456 24 420 6.68e-16 80.1
MS.gene052225.t1 MTR_4g127650 24.353 464 296 19 17 458 18 448 8.07e-16 80.1
MS.gene052225.t1 MTR_4g022290 24.943 441 254 22 30 446 35 422 8.64e-16 79.7
MS.gene052225.t1 MTR_3g086020 23.418 474 271 21 8 460 4 406 9.08e-16 79.3
MS.gene052225.t1 MTR_7g086000 23.585 424 277 10 17 420 12 408 2.06e-15 78.6
MS.gene052225.t1 MTR_2g104210 68.000 50 11 2 273 320 1 47 3.43e-15 70.5
MS.gene052225.t1 MTR_4g094832 26.518 313 198 13 156 460 133 421 7.58e-14 73.6
MS.gene052225.t1 MTR_8g041770 26.923 286 171 11 154 430 144 400 7.76e-14 73.6
MS.gene052225.t1 MTR_4g097090 24.444 450 276 17 17 444 17 424 8.03e-14 73.6
MS.gene052225.t1 MTR_7g014205 33.043 115 64 2 314 426 7 110 1.07e-13 68.2
MS.gene052225.t1 MTR_8g079710 24.036 441 262 18 40 456 41 432 9.72e-13 70.5
MS.gene052225.t1 MTR_7g014210 36.893 103 62 2 327 426 24 126 1.51e-12 65.9
MS.gene052225.t1 MTR_4g055170 25.416 421 268 21 15 423 18 404 6.07e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052225.t1 AT1G03940 33.264 481 278 19 10 469 9 467 3.12e-71 233
MS.gene052225.t1 AT3G29670 31.224 474 279 15 11 466 5 449 1.09e-56 194
MS.gene052225.t1 AT3G29635 31.858 452 275 13 28 468 27 456 1.98e-56 194
MS.gene052225.t1 AT3G29680 31.567 453 276 13 28 470 23 451 2.13e-56 194
MS.gene052225.t1 AT5G61160 29.032 465 294 14 10 461 4 445 8.06e-53 184
MS.gene052225.t1 AT3G29590 31.780 472 275 17 11 467 10 449 6.89e-48 171
MS.gene052225.t1 AT5G39090 28.326 466 299 13 12 467 8 448 8.13e-48 171
MS.gene052225.t1 AT5G39080 28.302 477 303 15 11 468 7 463 1.14e-47 171
MS.gene052225.t1 AT1G03495 33.427 356 203 15 9 351 8 342 1.38e-47 167
MS.gene052225.t1 AT5G39050 29.979 477 296 15 10 467 11 468 1.06e-43 160
MS.gene052225.t1 AT5G41040 24.408 422 259 16 13 421 21 395 3.51e-21 96.3
MS.gene052225.t1 AT5G48930 23.644 461 304 16 13 462 7 430 4.41e-21 95.9
MS.gene052225.t1 AT5G41040 24.408 422 259 16 13 421 37 411 4.88e-21 95.9
MS.gene052225.t1 AT5G67150 25.000 476 292 20 8 460 4 437 1.71e-19 91.3
MS.gene052225.t1 AT3G50270 23.992 471 288 19 6 450 3 429 2.03e-18 87.8
MS.gene052225.t1 AT5G63560 23.487 413 268 15 13 421 13 381 2.73e-18 87.4
MS.gene052225.t1 AT5G57840 24.583 480 298 17 10 468 4 440 5.51e-18 86.7
MS.gene052225.t1 AT2G19070 23.884 448 288 16 17 443 12 427 7.75e-18 86.3
MS.gene052225.t1 AT5G23940 25.652 460 271 20 30 455 25 447 9.45e-18 86.3
MS.gene052225.t1 AT5G42830 24.880 418 260 15 61 460 59 440 1.91e-17 85.1
MS.gene052225.t1 AT2G39980 24.152 501 290 22 11 460 10 471 4.96e-17 84.0
MS.gene052225.t1 AT5G38130 23.851 457 275 17 7 444 22 424 2.43e-16 81.6
MS.gene052225.t1 AT5G07850 24.528 477 299 21 7 460 6 444 4.23e-15 77.8
MS.gene052225.t1 AT1G27620 26.190 420 251 16 13 421 13 384 7.65e-15 77.0
MS.gene052225.t1 AT5G01210 24.947 473 296 18 12 453 9 453 1.13e-14 76.6
MS.gene052225.t1 AT5G07860 24.788 472 292 19 7 456 6 436 1.78e-14 75.9
MS.gene052225.t1 AT3G29690 31.333 150 98 3 320 467 33 179 5.61e-14 70.5
MS.gene052225.t1 AT5G07870 23.750 480 307 20 7 460 6 452 3.93e-12 68.6
MS.gene052225.t1 AT4G31910 22.917 384 245 12 11 375 11 362 1.99e-11 66.2

Find 73 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATACCAGAGGGGTATTT+TGG 0.151654 3.2:-74720061 MS.gene052225:CDS
ACAAATTTAGATAGAAATTT+TGG 0.182553 3.2:+74720202 None:intergenic
TGAGATGGTGCATGCGTTTA+AGG 0.195519 3.2:-74719789 MS.gene052225:CDS
AATGTTTATGAGACCGATTT+TGG 0.247173 3.2:-74719833 MS.gene052225:CDS
GGAGAAGGTGGACTTGAGTT+TGG 0.247995 3.2:-74719740 MS.gene052225:CDS
GCAATCATTTCATGAAATCT+TGG 0.347391 3.2:-74720504 MS.gene052225:CDS
TGCACCATCTCAACTTTAAC+AGG 0.358365 3.2:+74719800 None:intergenic
CAGTCTTCTACATCTTTAAG+AGG 0.376958 3.2:+74719923 None:intergenic
AGTTTATGTTCTGCTCCTAT+AGG 0.377376 3.2:+74721048 None:intergenic
GTTGTTGTTGTTCTTGTTGC+AGG 0.384008 3.2:+74721003 None:intergenic
CAGCTACATCACCTCCTTGA+TGG 0.384620 3.2:+74720473 None:intergenic
CACATTCACCCTTCCATTGT+TGG 0.385846 3.2:-74720605 MS.gene052225:CDS
GAGAAGGTGGACTTGAGTTT+GGG 0.395274 3.2:-74719739 MS.gene052225:CDS
AATAAGCGTTTAGCGTAGAT+TGG 0.403428 3.2:+74720940 None:intergenic
AGTAAAACTATGTATATGTC+TGG 0.416871 3.2:-74719890 MS.gene052225:CDS
GTCATTAATATCTTCAATCC+TGG 0.423090 3.2:+74720639 None:intergenic
TAAGAAATGGCAAAACCTAT+AGG 0.429096 3.2:-74721063 None:intergenic
GAAAGAGATTGTTTGAGAGA+TGG 0.430195 3.2:+74720868 None:intergenic
ATAAGATTCTCAAGTCCTCT+TGG 0.434524 3.2:+74720406 None:intergenic
CATCATTTCTTCCCTCTCGC+TGG 0.448126 3.2:-74720835 MS.gene052225:CDS
GGTGGTGTTTGTTGGAGAGA+TGG 0.452853 3.2:+74720713 None:intergenic
AGGTCTTCCAAATCCAAAAT+CGG 0.460413 3.2:+74719820 None:intergenic
CCCAACCATGGTGTTTGCGT+TGG 0.463801 3.2:-74720562 MS.gene052225:CDS
TAAGATTCTCAAGTCCTCTT+GGG 0.479124 3.2:+74720407 None:intergenic
AAAGGTGACGGAGTCTTCTT+CGG 0.484341 3.2:+74720765 None:intergenic
GCCAACGCAAACACCATGGT+TGG 0.488721 3.2:+74720561 None:intergenic
TGGTTGGTTGAATGAGGAAA+AGG 0.489129 3.2:+74720910 None:intergenic
GAGATGGTGCATGCGTTTAA+GGG 0.496036 3.2:-74719788 MS.gene052225:CDS
TTTGGAAAAGAAGAAATTGA+GGG 0.502317 3.2:-74720280 MS.gene052225:CDS
AGATATTAATGACATTGAAA+AGG 0.509159 3.2:-74720629 MS.gene052225:CDS
TGGGTTGGTGCTTAAAAGTG+AGG 0.512259 3.2:-74719720 MS.gene052225:CDS
CAATATTAACACACAAAACC+AGG 0.512389 3.2:-74720657 MS.gene052225:CDS
CCAACGCAAACACCATGGTT+GGG 0.516697 3.2:+74720562 None:intergenic
TGTTGTTAAGGTAATTGAAA+AGG 0.526288 3.2:-74719969 MS.gene052225:CDS
CTTGCAAAGCCAACAATGGA+AGG 0.529919 3.2:+74720596 None:intergenic
TCTTCGGTGCAGCGAATGAA+AGG 0.530046 3.2:+74720781 None:intergenic
GGAAATCAATTGTTTCTGCA+TGG 0.534473 3.2:-74719869 MS.gene052225:CDS
AAGTGCAACGATGAAGAGAA+AGG 0.537353 3.2:-74720020 MS.gene052225:CDS
TCTCTTGCTGAAAGTGGAGA+TGG 0.549776 3.2:-74719761 MS.gene052225:CDS
CTCTTAAAGATGTAGAAGAC+TGG 0.550312 3.2:-74719922 MS.gene052225:CDS
GGTGTGTCTCTTGCTGAAAG+TGG 0.551045 3.2:-74719767 MS.gene052225:CDS
GTCTCATAAACATTGAACTT+AGG 0.559774 3.2:+74719842 None:intergenic
AGGTGGACTTGAGTTTGGGT+TGG 0.563237 3.2:-74719735 MS.gene052225:CDS
CGATGATTCGATGATGGTAA+AGG 0.563360 3.2:+74720747 None:intergenic
GATGAAGAGAAAGGACATGA+AGG 0.568217 3.2:-74720011 MS.gene052225:CDS
GTTGCTCAAAGTCAAAGCAC+TGG 0.572117 3.2:-74720337 MS.gene052225:CDS
CAGTTTCCAAAATACCCCTC+TGG 0.574352 3.2:+74720055 None:intergenic
TTCGATGATGGTAAAGGTGA+CGG 0.584351 3.2:+74720753 None:intergenic
ATGAAGAGAAAGGACATGAA+GGG 0.596540 3.2:-74720010 MS.gene052225:CDS
GAAATGAGATTTCCAGCGAG+AGG 0.596654 3.2:+74720823 None:intergenic
CATTACATATTGTCACGTGA+TGG 0.601868 3.2:-74720539 MS.gene052225:CDS
ACATATTGTCACGTGATGGA+TGG 0.602963 3.2:-74720535 MS.gene052225:CDS
AAATGAGATTTCCAGCGAGA+GGG 0.605378 3.2:+74720824 None:intergenic
TTCTCTTCTCTCATTCAACA+AGG 0.605984 3.2:-74719686 MS.gene052225:CDS
CTTGGCTACTCAATACCAGA+GGG 0.607440 3.2:-74720070 MS.gene052225:CDS
GTCTCAATATTTCCCAACCA+TGG 0.616555 3.2:-74720574 MS.gene052225:CDS
ATCTTGCGATGATTCGATGA+TGG 0.618919 3.2:+74720741 None:intergenic
GAGACTTGCAAAGCCAACAA+TGG 0.623901 3.2:+74720592 None:intergenic
TGTGTGTTAATATTGGAACA+AGG 0.625336 3.2:+74720665 None:intergenic
TTGGAAAAGAAGAAATTGAG+GGG 0.626217 3.2:-74720279 MS.gene052225:CDS
GACCAGCAAAATGCAAGTCG+AGG 0.629102 3.2:+74720962 None:intergenic
GAAAGTGGAGATGGAGAAGG+TGG 0.639469 3.2:-74719752 MS.gene052225:CDS
AAGACCTGTTAAAGTTGAGA+TGG 0.641370 3.2:-74719804 MS.gene052225:CDS
TTGCAAAGCCAACAATGGAA+GGG 0.646182 3.2:+74720597 None:intergenic
TGTGATTGCAGAGATAGACT+TGG 0.654998 3.2:-74720088 MS.gene052225:CDS
AAAACTTCTCTATCAAAACA+AGG 0.666225 3.2:+74720436 None:intergenic
GCAATGCATAGAAATGATGA+CGG 0.667461 3.2:-74720142 MS.gene052225:CDS
TAATGCCAACGCAAACACCA+TGG 0.675041 3.2:+74720557 None:intergenic
TGCAAGTCGAGGAATGTGAG+AGG 0.687948 3.2:+74720973 None:intergenic
GCTGAAAGTGGAGATGGAGA+AGG 0.692838 3.2:-74719755 MS.gene052225:CDS
ACTTGGCTACTCAATACCAG+AGG 0.710749 3.2:-74720071 MS.gene052225:CDS
ACTTCTCTATCAAAACAAGG+TGG 0.730371 3.2:+74720439 None:intergenic
TTGGCTACTCAATACCAGAG+GGG 0.789894 3.2:-74720069 MS.gene052225:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACAAATTTAGATAGAAATTT+TGG + chr3.2:74720522-74720541 None:intergenic 15.0%
!!! ATTTTTTTACAAGAAAATCT+TGG - chr3.2:74720349-74720368 MS.gene052225:CDS 15.0%
!!! TTTAAGGTAATTATTGTTTT+TGG - chr3.2:74720423-74720442 MS.gene052225:CDS 15.0%
!! AGATATTAATGACATTGAAA+AGG - chr3.2:74720092-74720111 MS.gene052225:CDS 20.0%
!!! TATTTTCTTTGAGATTTTTC+AGG + chr3.2:74720032-74720051 None:intergenic 20.0%
! AAAACTTCTCTATCAAAACA+AGG + chr3.2:74720288-74720307 None:intergenic 25.0%
! AGTAAAACTATGTATATGTC+TGG - chr3.2:74720831-74720850 MS.gene052225:CDS 25.0%
! TGTTGTTAAGGTAATTGAAA+AGG - chr3.2:74720752-74720771 MS.gene052225:CDS 25.0%
! TTTGGAAAAGAAGAAATTGA+GGG - chr3.2:74720441-74720460 MS.gene052225:CDS 25.0%
!! AGAACATGAAGATTCTTTTA+AGG - chr3.2:74720407-74720426 MS.gene052225:CDS 25.0%
!! TTGTAACTAGTGCTTTTATT+TGG - chr3.2:74720541-74720560 MS.gene052225:CDS 25.0%
!!! TAGTTTTGTTGATGTTGTTA+AGG - chr3.2:74720740-74720759 MS.gene052225:CDS 25.0%
!!! TGTAACTAGTGCTTTTATTT+GGG - chr3.2:74720542-74720561 MS.gene052225:CDS 25.0%
!!! TTTTGATAGAGAAGTTTTGA+GGG - chr3.2:74720290-74720309 MS.gene052225:CDS 25.0%
!!! TTTTGGAAAAGAAGAAATTG+AGG - chr3.2:74720440-74720459 MS.gene052225:CDS 25.0%
AAATTGAGGGGATGAAAAAA+TGG - chr3.2:74720454-74720473 MS.gene052225:CDS 30.0%
AATTGAGGGGATGAAAAAAT+GGG - chr3.2:74720455-74720474 MS.gene052225:CDS 30.0%
CAATATTAACACACAAAACC+AGG - chr3.2:74720064-74720083 MS.gene052225:CDS 30.0%
GCAATCATTTCATGAAATCT+TGG - chr3.2:74720217-74720236 MS.gene052225:CDS 30.0%
GTCATTAATATCTTCAATCC+TGG + chr3.2:74720085-74720104 None:intergenic 30.0%
GTCTCATAAACATTGAACTT+AGG + chr3.2:74720882-74720901 None:intergenic 30.0%
TGTGTGTTAATATTGGAACA+AGG + chr3.2:74720059-74720078 None:intergenic 30.0%
TTGGAAAAGAAGAAATTGAG+GGG - chr3.2:74720442-74720461 MS.gene052225:CDS 30.0%
!! AATGTTTATGAGACCGATTT+TGG - chr3.2:74720888-74720907 MS.gene052225:CDS 30.0%
!! GTTTTGATAGAGAAGTTTTG+AGG - chr3.2:74720289-74720308 MS.gene052225:CDS 30.0%
!!! CTGGTTTTGTGTGTTAATAT+TGG + chr3.2:74720066-74720085 None:intergenic 30.0%
!!! GAAGTGTTGTTTTGCAAAAA+TGG + chr3.2:74719834-74719853 None:intergenic 30.0%
!!! TGTTGTTTTGCAAAAATGGT+TGG + chr3.2:74719830-74719849 None:intergenic 30.0%
!!! TTTCTTTGAGATTTTTCAGG+TGG + chr3.2:74720029-74720048 None:intergenic 30.0%
AAGACCTGTTAAAGTTGAGA+TGG - chr3.2:74720917-74720936 MS.gene052225:CDS 35.0%
AATAAGCGTTTAGCGTAGAT+TGG + chr3.2:74719784-74719803 None:intergenic 35.0%
ACTTCTCTATCAAAACAAGG+TGG + chr3.2:74720285-74720304 None:intergenic 35.0%
AGGTCTTCCAAATCCAAAAT+CGG + chr3.2:74720904-74720923 None:intergenic 35.0%
AGTTTATGTTCTGCTCCTAT+AGG + chr3.2:74719676-74719695 None:intergenic 35.0%
ATAAGATTCTCAAGTCCTCT+TGG + chr3.2:74720318-74720337 None:intergenic 35.0%
ATGAAGAGAAAGGACATGAA+GGG - chr3.2:74720711-74720730 MS.gene052225:CDS 35.0%
CAGTCTTCTACATCTTTAAG+AGG + chr3.2:74720801-74720820 None:intergenic 35.0%
CATTACATATTGTCACGTGA+TGG - chr3.2:74720182-74720201 MS.gene052225:CDS 35.0%
CTCTTAAAGATGTAGAAGAC+TGG - chr3.2:74720799-74720818 MS.gene052225:CDS 35.0%
GAAAGAGATTGTTTGAGAGA+TGG + chr3.2:74719856-74719875 None:intergenic 35.0%
GCAATGCATAGAAATGATGA+CGG - chr3.2:74720579-74720598 MS.gene052225:CDS 35.0%
TAAGATTCTCAAGTCCTCTT+GGG + chr3.2:74720317-74720336 None:intergenic 35.0%
TTCTCTTCTCTCATTCAACA+AGG - chr3.2:74721035-74721054 MS.gene052225:CDS 35.0%
! CAAAAATGGTTGGTTGAATG+AGG + chr3.2:74719820-74719839 None:intergenic 35.0%
!! AAAAATGGGTGCTGAATCAA+TGG - chr3.2:74720469-74720488 MS.gene052225:CDS 35.0%
!! GGAAATCAATTGTTTCTGCA+TGG - chr3.2:74720852-74720871 MS.gene052225:CDS 35.0%
!! TATGAGACCGATTTTGGATT+TGG - chr3.2:74720894-74720913 MS.gene052225:CDS 35.0%
!! TCTTGGTCTTTTATCCATCA+AGG - chr3.2:74720234-74720253 MS.gene052225:CDS 35.0%
!!! ATTTTTCAGGTGGTGTTTGT+TGG + chr3.2:74720019-74720038 None:intergenic 35.0%
!!! TGCTTTTATTTGGGCTAGTA+TGG - chr3.2:74720551-74720570 MS.gene052225:CDS 35.0%
AAATGAGATTTCCAGCGAGA+GGG + chr3.2:74719900-74719919 None:intergenic 40.0%
AAGTGCAACGATGAAGAGAA+AGG - chr3.2:74720701-74720720 MS.gene052225:CDS 40.0%
ATCTTGCGATGATTCGATGA+TGG + chr3.2:74719983-74720002 None:intergenic 40.0%
GATGAAGAGAAAGGACATGA+AGG - chr3.2:74720710-74720729 MS.gene052225:CDS 40.0%
GTCTCAATATTTCCCAACCA+TGG - chr3.2:74720147-74720166 MS.gene052225:CDS 40.0%
TGCACCATCTCAACTTTAAC+AGG + chr3.2:74720924-74720943 None:intergenic 40.0%
TGTGATTGCAGAGATAGACT+TGG - chr3.2:74720633-74720652 MS.gene052225:CDS 40.0%
TTGCAAAGCCAACAATGGAA+GGG + chr3.2:74720127-74720146 None:intergenic 40.0%
! ACATATTGTCACGTGATGGA+TGG - chr3.2:74720186-74720205 MS.gene052225:CDS 40.0%
! ATGAAAGGTTTTTGTGGTGG+TGG + chr3.2:74719928-74719947 None:intergenic 40.0%
! CAGCGAATGAAAGGTTTTTG+TGG + chr3.2:74719934-74719953 None:intergenic 40.0%
! CGAATGAAAGGTTTTTGTGG+TGG + chr3.2:74719931-74719950 None:intergenic 40.0%
! CGATGATTCGATGATGGTAA+AGG + chr3.2:74719977-74719996 None:intergenic 40.0%
! GTTGTTGTTGTTCTTGTTGC+AGG + chr3.2:74719721-74719740 None:intergenic 40.0%
! TGGTCTTTTATCCATCAAGG+AGG - chr3.2:74720237-74720256 MS.gene052225:CDS 40.0%
! TGGTTGGTTGAATGAGGAAA+AGG + chr3.2:74719814-74719833 None:intergenic 40.0%
! TTCGATGATGGTAAAGGTGA+CGG + chr3.2:74719971-74719990 None:intergenic 40.0%
!!! TCAATACCAGAGGGGTATTT+TGG - chr3.2:74720660-74720679 MS.gene052225:CDS 40.0%
ACTTGGCTACTCAATACCAG+AGG - chr3.2:74720650-74720669 MS.gene052225:CDS 45.0%
CACATTCACCCTTCCATTGT+TGG - chr3.2:74720116-74720135 MS.gene052225:CDS 45.0%
CAGTTTCCAAAATACCCCTC+TGG + chr3.2:74720669-74720688 None:intergenic 45.0%
CTTGCAAAGCCAACAATGGA+AGG + chr3.2:74720128-74720147 None:intergenic 45.0%
CTTGGCTACTCAATACCAGA+GGG - chr3.2:74720651-74720670 MS.gene052225:CDS 45.0%
GAAATGAGATTTCCAGCGAG+AGG + chr3.2:74719901-74719920 None:intergenic 45.0%
GAGACTTGCAAAGCCAACAA+TGG + chr3.2:74720132-74720151 None:intergenic 45.0%
GAGATGGTGCATGCGTTTAA+GGG - chr3.2:74720933-74720952 MS.gene052225:CDS 45.0%
GTTGCTCAAAGTCAAAGCAC+TGG - chr3.2:74720384-74720403 MS.gene052225:CDS 45.0%
TAATGCCAACGCAAACACCA+TGG + chr3.2:74720167-74720186 None:intergenic 45.0%
TCTCTTGCTGAAAGTGGAGA+TGG - chr3.2:74720960-74720979 MS.gene052225:CDS 45.0%
TGAGATGGTGCATGCGTTTA+AGG - chr3.2:74720932-74720951 MS.gene052225:CDS 45.0%
TTGGCTACTCAATACCAGAG+GGG - chr3.2:74720652-74720671 MS.gene052225:CDS 45.0%
! GAGAAGGTGGACTTGAGTTT+GGG - chr3.2:74720982-74721001 MS.gene052225:CDS 45.0%
!! AAAGGTGACGGAGTCTTCTT+CGG + chr3.2:74719959-74719978 None:intergenic 45.0%
!! TGGGTTGGTGCTTAAAAGTG+AGG - chr3.2:74721001-74721020 MS.gene052225:CDS 45.0%
!! TTCCTCGACTTGCATTTTGC+TGG - chr3.2:74719757-74719776 MS.gene052225:CDS 45.0%
!!! AAAGGTTTTTGTGGTGGTGG+TGG + chr3.2:74719925-74719944 None:intergenic 45.0%
CAGCTACATCACCTCCTTGA+TGG + chr3.2:74720251-74720270 None:intergenic 50.0%
CATCATTTCTTCCCTCTCGC+TGG - chr3.2:74719886-74719905 MS.gene052225:CDS 50.0%
CCAACGCAAACACCATGGTT+GGG + chr3.2:74720162-74720181 None:intergenic 50.0%
GAAAGTGGAGATGGAGAAGG+TGG - chr3.2:74720969-74720988 MS.gene052225:CDS 50.0%
GACCAGCAAAATGCAAGTCG+AGG + chr3.2:74719762-74719781 None:intergenic 50.0%
GCTGAAAGTGGAGATGGAGA+AGG - chr3.2:74720966-74720985 MS.gene052225:CDS 50.0%
GGTGTGTCTCTTGCTGAAAG+TGG - chr3.2:74720954-74720973 MS.gene052225:CDS 50.0%
TGCAAGTCGAGGAATGTGAG+AGG + chr3.2:74719751-74719770 None:intergenic 50.0%
! AGGTGGACTTGAGTTTGGGT+TGG - chr3.2:74720986-74721005 MS.gene052225:CDS 50.0%
! GGAGAAGGTGGACTTGAGTT+TGG - chr3.2:74720981-74721000 MS.gene052225:CDS 50.0%
! GGTGGTGTTTGTTGGAGAGA+TGG + chr3.2:74720011-74720030 None:intergenic 50.0%
! TCTTCGGTGCAGCGAATGAA+AGG + chr3.2:74719943-74719962 None:intergenic 50.0%
!!! GAAGTTTTGAGGGACCCAAG+AGG - chr3.2:74720300-74720319 MS.gene052225:CDS 50.0%
CCCAACCATGGTGTTTGCGT+TGG - chr3.2:74720159-74720178 MS.gene052225:CDS 55.0%
GCCAACGCAAACACCATGGT+TGG + chr3.2:74720163-74720182 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 74719664 74721079 74719664 ID=MS.gene052225
chr3.2 mRNA 74719664 74721079 74719664 ID=MS.gene052225.t1;Parent=MS.gene052225
chr3.2 exon 74719664 74721079 74719664 ID=MS.gene052225.t1.exon1;Parent=MS.gene052225.t1
chr3.2 CDS 74719664 74721079 74719664 ID=cds.MS.gene052225.t1;Parent=MS.gene052225.t1
Gene Sequence

>MS.gene052225

ATGGCAAAACCTATAGGAGCAGAACATAAACTTATTGAGCAAACTCTTGTGTTTCCTGCAACAAGAACAACAACAACTTCTCTTCCTCTCACATTCCTCGACTTGCATTTTGCTGGTCCAATCTACGCTAAACGCTTATTCTTCTATCCTTTTCCTCATTCAACCAACCATTTTTGCAAAACAACACTTCCATCTCTCAAACAATCTCTTTCTTGTGTTCTTCATCATTTCTTCCCTCTCGCTGGAAATCTCATTTCTCCACCACCACCACAAAAACCTTTCATTCGCTGCACCGAAGAAGACTCCGTCACCTTTACCATCATCGAATCATCGCAAGATTTCAACCATCTCTCCAACAAACACCACCTGAAAAATCTCAAAGAAAATAATAACCTTGTTCCAATATTAACACACAAAACCAGGATTGAAGATATTAATGACATTGAAAAGGACACATTCACCCTTCCATTGTTGGCTTTGCAAGTCTCAATATTTCCCAACCATGGTGTTTGCGTTGGCATTACATATTGTCACGTGATGGATGGTAACAGTTGCAATCATTTCATGAAATCTTGGTCTTTTATCCATCAAGGAGGTGATGTAGCTGAATTGAAGTCACCACCTTGTTTTGATAGAGAAGTTTTGAGGGACCCAAGAGGACTTGAGAATCTTATTTTAAGAGATTATTTTTTTACAAGAAAATCTTGGAAGTTTAGACTCGTTGCTCAAAGTCAAAGCACTGGAGAACATGAAGATTCTTTTAAGGTAATTATTGTTTTTGGAAAAGAAGAAATTGAGGGGATGAAAAAATGGGTGCTGAATCAATGGAAGAAAAATGATAATGAAATCAACGTGCCAAAATTTCTATCTAAATTTGTTGTAACTAGTGCTTTTATTTGGGCTAGTATGGTTAAAGCAATGCATAGAAATGATGACGGTGATGAAAAAGATGAGTACTTTTGTTTCACATGTGATTGCAGAGATAGACTTGGCTACTCAATACCAGAGGGGTATTTTGGAAACTGTGTAGTGTCTAAAAGTGCAACGATGAAGAGAAAGGACATGAAGGGAAAAAATAGTTTTGTTGATGTTGTTAAGGTAATTGAAAAGGCTGTTAATGAGATAAAAAATGAACCTCTTAAAGATGTAGAAGACTGGTTTGAATTAAGTAAAACTATGTATATGTCTGGAAATCAATTGTTTCTGCATGGATCACCTAAGTTCAATGTTTATGAGACCGATTTTGGATTTGGAAGACCTGTTAAAGTTGAGATGGTGCATGCGTTTAAGGGTGTGTCTCTTGCTGAAAGTGGAGATGGAGAAGGTGGACTTGAGTTTGGGTTGGTGCTTAAAAGTGAGGAATTTGAATATTTCTCTTCTCTCATTCAACAAGGACTTGAAGTTTTTAAACATTAA
Protein sequence

>MS.gene052225.t1

MAKPIGAEHKLIEQTLVFPATRTTTTSLPLTFLDLHFAGPIYAKRLFFYPFPHSTNHFCKTTLPSLKQSLSCVLHHFFPLAGNLISPPPPQKPFIRCTEEDSVTFTIIESSQDFNHLSNKHHLKNLKENNNLVPILTHKTRIEDINDIEKDTFTLPLLALQVSIFPNHGVCVGITYCHVMDGNSCNHFMKSWSFIHQGGDVAELKSPPCFDREVLRDPRGLENLILRDYFFTRKSWKFRLVAQSQSTGEHEDSFKVIIVFGKEEIEGMKKWVLNQWKKNDNEINVPKFLSKFVVTSAFIWASMVKAMHRNDDGDEKDEYFCFTCDCRDRLGYSIPEGYFGNCVVSKSATMKRKDMKGKNSFVDVVKVIEKAVNEIKNEPLKDVEDWFELSKTMYMSGNQLFLHGSPKFNVYETDFGFGRPVKVEMVHAFKGVSLAESGDGEGGLEFGLVLKSEEFEYFSSLIQQGLEVFKH