Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79556.t1 | XP_003621270.1 | 92.3 | 195 | 11 | 2 | 1 | 195 | 119 | 309 | 3.00E-90 | 341.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79556.t1 | Q9LXF1 | 44.2 | 217 | 90 | 6 | 1 | 195 | 119 | 326 | 1.8e-22 | 107.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79556.t1 | G7L225 | 92.3 | 195 | 11 | 2 | 1 | 195 | 119 | 309 | 2.2e-90 | 341.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051789 | MS.gene79556 | 0.805859 | 1.09E-49 | -1.69E-46 |
| MS.gene053074 | MS.gene79556 | 0.801913 | 7.19E-49 | -1.69E-46 |
| MS.gene055133 | MS.gene79556 | 0.804308 | 2.30E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79556.t1 | MTR_7g011170 | 91.795 | 195 | 12 | 2 | 1 | 195 | 119 | 309 | 2.23e-114 | 328 |
| MS.gene79556.t1 | MTR_6g012690 | 47.082 | 257 | 56 | 11 | 1 | 195 | 119 | 357 | 1.88e-36 | 130 |
| MS.gene79556.t1 | MTR_4g082290 | 63.333 | 60 | 16 | 3 | 1 | 55 | 119 | 177 | 2.32e-13 | 67.8 |
| MS.gene79556.t1 | MTR_4g082230 | 63.333 | 60 | 16 | 3 | 1 | 55 | 119 | 177 | 2.55e-13 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79556.t1 | AT5G15310 | 45.161 | 217 | 88 | 7 | 1 | 195 | 40 | 247 | 3.04e-34 | 122 |
| MS.gene79556.t1 | AT5G15310 | 45.933 | 209 | 87 | 7 | 1 | 195 | 119 | 315 | 1.45e-33 | 122 |
| MS.gene79556.t1 | AT5G15310 | 45.161 | 217 | 88 | 7 | 1 | 195 | 119 | 326 | 3.58e-33 | 121 |
| MS.gene79556.t1 | AT3G01140 | 38.559 | 236 | 95 | 8 | 1 | 195 | 119 | 345 | 1.72e-26 | 103 |
| MS.gene79556.t1 | AT3G01140 | 39.496 | 238 | 90 | 10 | 1 | 195 | 162 | 388 | 2.88e-26 | 103 |
| MS.gene79556.t1 | AT5G15310 | 76.271 | 59 | 12 | 2 | 1 | 58 | 119 | 176 | 4.27e-17 | 77.0 |
Find 33 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTACACTCTCCACCGCTTTC+CGG | 0.265479 | 7.3:+86906964 | None:intergenic |
| GCTAAGATTTGCAGCATTCT+TGG | 0.289751 | 7.3:+86907350 | None:intergenic |
| GTTAGAATTCAAAGATTTGA+TGG | 0.315601 | 7.3:-86907109 | MS.gene79556:CDS |
| ACTTGAAGCTGAAGCAAGAT+TGG | 0.402581 | 7.3:-86907301 | MS.gene79556:CDS |
| AAGAAGAAGGTGAGAAAGAG+TGG | 0.428852 | 7.3:-86907158 | MS.gene79556:CDS |
| ATCTTAGCCACAAGGCTCAA+TGG | 0.433665 | 7.3:-86907335 | MS.gene79556:CDS |
| TCTTAGCCACAAGGCTCAAT+GGG | 0.448848 | 7.3:-86907334 | MS.gene79556:CDS |
| TTTGATGGAGAATTCATCAA+TGG | 0.459211 | 7.3:-86907094 | MS.gene79556:CDS |
| ATGAAGATAACAAGAACTAC+TGG | 0.460330 | 7.3:-86906900 | MS.gene79556:CDS |
| CGCTTTCCGGTGACAAACTC+AGG | 0.463872 | 7.3:+86906977 | None:intergenic |
| CACTTTCCCATTGAGCCTTG+TGG | 0.471844 | 7.3:+86907328 | None:intergenic |
| CAATTGCAACAGCATCAACT+TGG | 0.472770 | 7.3:-86907255 | MS.gene79556:CDS |
| TCATCTTCAAATCAAGTACT+TGG | 0.499747 | 7.3:-86907186 | MS.gene79556:CDS |
| GATTTGCAGCATTCTTGGAA+TGG | 0.519998 | 7.3:+86907355 | None:intergenic |
| GATGGAAGTGGTAGCGGCGG+TGG | 0.528157 | 7.3:-86906934 | MS.gene79556:CDS |
| ACTTGTGATGGAAGTGGTAG+CGG | 0.531173 | 7.3:-86906940 | MS.gene79556:CDS |
| GTACTTGGTATTAAAGAAGA+AGG | 0.544001 | 7.3:-86907171 | MS.gene79556:CDS |
| TAGAACATTGGCGAATCAGA+AGG | 0.556955 | 7.3:+86906847 | None:intergenic |
| TTGCTTGATAATTCTAACTC+CGG | 0.569468 | 7.3:-86907003 | MS.gene79556:CDS |
| ATGATCAGTGCTGCTGAGGA+GGG | 0.581711 | 7.3:-86907039 | MS.gene79556:CDS |
| CGGTGACCTGAGTTTGTCAC+CGG | 0.584452 | 7.3:-86906983 | MS.gene79556:CDS |
| AATGATCAGTGCTGCTGAGG+AGG | 0.587023 | 7.3:-86907040 | MS.gene79556:CDS |
| GGAGAGTGTAATACTTGTGA+TGG | 0.589020 | 7.3:-86906952 | MS.gene79556:CDS |
| CGGTGACAAACTCAGGTCAC+CGG | 0.590245 | 7.3:+86906984 | None:intergenic |
| GTAGATGATGAAGGTTGTGA+TGG | 0.594359 | 7.3:+86907213 | None:intergenic |
| GATGATGATGTAGATGATGA+AGG | 0.598426 | 7.3:+86907204 | None:intergenic |
| GAAGGTGAGAAAGAGTGGAA+AGG | 0.602261 | 7.3:-86907153 | MS.gene79556:CDS |
| TGTAATACTTGTGATGGAAG+TGG | 0.616978 | 7.3:-86906946 | MS.gene79556:CDS |
| CTGAGTTTGTCACCGGAAAG+CGG | 0.631632 | 7.3:-86906976 | MS.gene79556:CDS |
| TGCTGCAAATCTTAGCCACA+AGG | 0.636234 | 7.3:-86907343 | MS.gene79556:CDS |
| GACAATGATCAGTGCTGCTG+AGG | 0.665799 | 7.3:-86907043 | MS.gene79556:CDS |
| AGTTTGTCACCGGAAAGCGG+TGG | 0.707277 | 7.3:-86906973 | MS.gene79556:CDS |
| TGTGATGGAAGTGGTAGCGG+CGG | 0.710359 | 7.3:-86906937 | MS.gene79556:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GTTAGAATTCAAAGATTTGA+TGG | - | chr7.3:86907148-86907167 | MS.gene79556:CDS | 25.0% |
| ATGAAGATAACAAGAACTAC+TGG | - | chr7.3:86907357-86907376 | MS.gene79556:CDS | 30.0% | |
| GTACTTGGTATTAAAGAAGA+AGG | - | chr7.3:86907086-86907105 | MS.gene79556:CDS | 30.0% | |
| TCATCTTCAAATCAAGTACT+TGG | - | chr7.3:86907071-86907090 | MS.gene79556:CDS | 30.0% | |
| TTTGATGGAGAATTCATCAA+TGG | - | chr7.3:86907163-86907182 | MS.gene79556:CDS | 30.0% | |
| ! | TTGCTTGATAATTCTAACTC+CGG | - | chr7.3:86907254-86907273 | MS.gene79556:CDS | 30.0% |
| !!! | GGAAAAAGGGTTTCATTTTT+TGG | + | chr7.3:86906876-86906895 | None:intergenic | 30.0% |
| ! | GATGATGATGTAGATGATGA+AGG | + | chr7.3:86907056-86907075 | None:intergenic | 35.0% |
| ! | TGTAATACTTGTGATGGAAG+TGG | - | chr7.3:86907311-86907330 | MS.gene79556:CDS | 35.0% |
| !! | GGAATGGTTTTCAGGAAAAA+GGG | + | chr7.3:86906889-86906908 | None:intergenic | 35.0% |
| !! | TGGAATGGTTTTCAGGAAAA+AGG | + | chr7.3:86906890-86906909 | None:intergenic | 35.0% |
| !!! | TTTTTTGGCTTGTGTGTAAC+AGG | + | chr7.3:86906861-86906880 | None:intergenic | 35.0% |
| AAGAAGAAGGTGAGAAAGAG+TGG | - | chr7.3:86907099-86907118 | MS.gene79556:CDS | 40.0% | |
| ACTTGAAGCTGAAGCAAGAT+TGG | - | chr7.3:86906956-86906975 | MS.gene79556:CDS | 40.0% | |
| CAATTGCAACAGCATCAACT+TGG | - | chr7.3:86907002-86907021 | MS.gene79556:CDS | 40.0% | |
| GATTTGCAGCATTCTTGGAA+TGG | + | chr7.3:86906905-86906924 | None:intergenic | 40.0% | |
| GCTAAGATTTGCAGCATTCT+TGG | + | chr7.3:86906910-86906929 | None:intergenic | 40.0% | |
| GTAGATGATGAAGGTTGTGA+TGG | + | chr7.3:86907047-86907066 | None:intergenic | 40.0% | |
| TAGAACATTGGCGAATCAGA+AGG | + | chr7.3:86907413-86907432 | None:intergenic | 40.0% | |
| ! | GCATTCTTGGAATGGTTTTC+AGG | + | chr7.3:86906897-86906916 | None:intergenic | 40.0% |
| ! | GGAGAGTGTAATACTTGTGA+TGG | - | chr7.3:86907305-86907324 | MS.gene79556:CDS | 40.0% |
| ATCTTAGCCACAAGGCTCAA+TGG | - | chr7.3:86906922-86906941 | MS.gene79556:CDS | 45.0% | |
| GAAGGTGAGAAAGAGTGGAA+AGG | - | chr7.3:86907104-86907123 | MS.gene79556:CDS | 45.0% | |
| TCTTAGCCACAAGGCTCAAT+GGG | - | chr7.3:86906923-86906942 | MS.gene79556:CDS | 45.0% | |
| TGCTGCAAATCTTAGCCACA+AGG | - | chr7.3:86906914-86906933 | MS.gene79556:CDS | 45.0% | |
| ! | ACTTGTGATGGAAGTGGTAG+CGG | - | chr7.3:86907317-86907336 | MS.gene79556:CDS | 45.0% |
| CACTTTCCCATTGAGCCTTG+TGG | + | chr7.3:86906932-86906951 | None:intergenic | 50.0% | |
| CTGAGTTTGTCACCGGAAAG+CGG | - | chr7.3:86907281-86907300 | MS.gene79556:CDS | 50.0% | |
| TTACACTCTCCACCGCTTTC+CGG | + | chr7.3:86907296-86907315 | None:intergenic | 50.0% | |
| ! | AATGATCAGTGCTGCTGAGG+AGG | - | chr7.3:86907217-86907236 | MS.gene79556:CDS | 50.0% |
| ! | ATGATCAGTGCTGCTGAGGA+GGG | - | chr7.3:86907218-86907237 | MS.gene79556:CDS | 50.0% |
| ! | GACAATGATCAGTGCTGCTG+AGG | - | chr7.3:86907214-86907233 | MS.gene79556:CDS | 50.0% |
| AGTTTGTCACCGGAAAGCGG+TGG | - | chr7.3:86907284-86907303 | MS.gene79556:CDS | 55.0% | |
| CGCTTTCCGGTGACAAACTC+AGG | + | chr7.3:86907283-86907302 | None:intergenic | 55.0% | |
| CGGTGACAAACTCAGGTCAC+CGG | + | chr7.3:86907276-86907295 | None:intergenic | 55.0% | |
| CGGTGACCTGAGTTTGTCAC+CGG | - | chr7.3:86907274-86907293 | MS.gene79556:CDS | 55.0% | |
| !! | TGTGATGGAAGTGGTAGCGG+CGG | - | chr7.3:86907320-86907339 | MS.gene79556:CDS | 55.0% |
| !! | GATGGAAGTGGTAGCGGCGG+TGG | - | chr7.3:86907323-86907342 | MS.gene79556:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 86906846 | 86907433 | 86906846 | ID=MS.gene79556 |
| chr7.3 | mRNA | 86906846 | 86907433 | 86906846 | ID=MS.gene79556.t1;Parent=MS.gene79556 |
| chr7.3 | exon | 86906846 | 86907433 | 86906846 | ID=MS.gene79556.t1.exon1;Parent=MS.gene79556.t1 |
| chr7.3 | CDS | 86906846 | 86907433 | 86906846 | ID=cds.MS.gene79556.t1;Parent=MS.gene79556.t1 |
| Gene Sequence |
| Protein sequence |
