Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80032.t1 | RHN82715.1 | 75.8 | 223 | 31 | 2 | 1 | 223 | 1 | 200 | 3.70E-80 | 308.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80032.t1 | A0A396K533 | 75.8 | 223 | 31 | 2 | 1 | 223 | 1 | 200 | 2.7e-80 | 308.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057897 | MS.gene80032 | 0.915247 | 7.38E-85 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80032.t1 | MTR_1g115455 | 71.542 | 253 | 29 | 2 | 1 | 253 | 1 | 210 | 1.93e-121 | 345 |
| MS.gene80032.t1 | MTR_8g445860 | 66.000 | 250 | 43 | 4 | 4 | 253 | 8 | 215 | 5.92e-105 | 304 |
| MS.gene80032.t1 | MTR_0150s0010 | 57.087 | 254 | 66 | 5 | 2 | 253 | 3 | 215 | 3.20e-80 | 241 |
| MS.gene80032.t1 | MTR_1g115440 | 54.297 | 256 | 70 | 6 | 2 | 253 | 4 | 216 | 4.06e-80 | 241 |
| MS.gene80032.t1 | MTR_8g044880 | 44.710 | 293 | 79 | 4 | 3 | 253 | 2 | 253 | 7.91e-68 | 211 |
| MS.gene80032.t1 | MTR_8g445910 | 44.366 | 284 | 75 | 4 | 3 | 244 | 2 | 244 | 2.48e-64 | 202 |
| MS.gene80032.t1 | MTR_1g115450 | 76.800 | 125 | 29 | 0 | 4 | 128 | 2 | 126 | 1.36e-63 | 196 |
| MS.gene80032.t1 | MTR_0150s0030 | 49.438 | 178 | 45 | 2 | 3 | 135 | 2 | 179 | 4.48e-45 | 150 |
| MS.gene80032.t1 | MTR_8g445920 | 74.074 | 81 | 21 | 0 | 2 | 82 | 3 | 83 | 1.55e-35 | 123 |
| MS.gene80032.t1 | MTR_8g044890 | 74.074 | 81 | 21 | 0 | 2 | 82 | 3 | 83 | 1.55e-35 | 123 |
| MS.gene80032.t1 | MTR_1g115435 | 33.205 | 259 | 89 | 9 | 4 | 253 | 2 | 185 | 2.33e-31 | 115 |
| MS.gene80032.t1 | MTR_1g115470 | 55.046 | 109 | 46 | 1 | 5 | 113 | 2 | 107 | 4.06e-31 | 119 |
| MS.gene80032.t1 | MTR_1g115470 | 37.063 | 143 | 48 | 4 | 113 | 253 | 181 | 283 | 5.59e-17 | 80.1 |
| MS.gene80032.t1 | MTR_7g012680 | 29.130 | 230 | 109 | 5 | 9 | 233 | 14 | 194 | 4.58e-23 | 97.4 |
| MS.gene80032.t1 | MTR_8g445870 | 41.935 | 124 | 44 | 4 | 4 | 122 | 2 | 102 | 1.92e-22 | 89.0 |
| MS.gene80032.t1 | MTR_7g066570 | 27.459 | 244 | 122 | 6 | 10 | 247 | 15 | 209 | 8.43e-21 | 91.7 |
| MS.gene80032.t1 | MTR_7g066340 | 26.275 | 255 | 135 | 6 | 2 | 251 | 5 | 211 | 9.69e-21 | 91.3 |
| MS.gene80032.t1 | MTR_7g058710 | 28.295 | 258 | 129 | 10 | 1 | 254 | 1 | 206 | 2.13e-19 | 87.4 |
| MS.gene80032.t1 | MTR_1g014060 | 27.869 | 244 | 116 | 9 | 19 | 254 | 10 | 201 | 7.40e-18 | 82.4 |
| MS.gene80032.t1 | MTR_7g066260 | 24.111 | 253 | 137 | 6 | 9 | 254 | 15 | 219 | 2.64e-17 | 81.3 |
| MS.gene80032.t1 | MTR_7g066350 | 26.255 | 259 | 138 | 7 | 1 | 254 | 1 | 211 | 8.45e-17 | 79.7 |
| MS.gene80032.t1 | MTR_7g063900 | 25.097 | 259 | 137 | 8 | 2 | 254 | 3 | 210 | 1.37e-16 | 79.0 |
| MS.gene80032.t1 | MTR_7g066290 | 23.904 | 251 | 140 | 6 | 9 | 254 | 15 | 219 | 1.47e-16 | 79.0 |
| MS.gene80032.t1 | MTR_1g041315 | 26.587 | 252 | 129 | 8 | 9 | 253 | 14 | 216 | 3.25e-16 | 78.2 |
| MS.gene80032.t1 | MTR_4g019310 | 27.820 | 266 | 134 | 9 | 3 | 254 | 2 | 223 | 5.28e-16 | 77.4 |
| MS.gene80032.t1 | MTR_7g063660 | 27.692 | 260 | 129 | 8 | 1 | 254 | 1 | 207 | 7.18e-16 | 77.0 |
| MS.gene80032.t1 | MTR_7g063880 | 27.547 | 265 | 126 | 10 | 1 | 254 | 1 | 210 | 8.02e-16 | 76.6 |
| MS.gene80032.t1 | MTR_7g062280 | 30.159 | 252 | 133 | 10 | 9 | 254 | 10 | 224 | 1.08e-15 | 76.6 |
| MS.gene80032.t1 | MTR_7g063890 | 23.938 | 259 | 141 | 7 | 1 | 254 | 19 | 226 | 1.59e-15 | 75.9 |
| MS.gene80032.t1 | MTR_4g017410 | 24.354 | 271 | 142 | 8 | 1 | 254 | 1 | 225 | 3.18e-15 | 75.1 |
| MS.gene80032.t1 | MTR_7g063840 | 33.962 | 159 | 91 | 5 | 10 | 159 | 11 | 164 | 7.19e-15 | 73.9 |
| MS.gene80032.t1 | MTR_4g019340 | 26.336 | 262 | 132 | 7 | 10 | 254 | 39 | 256 | 8.31e-15 | 73.9 |
| MS.gene80032.t1 | MTR_4g019340 | 39.241 | 79 | 46 | 1 | 9 | 87 | 303 | 379 | 3.92e-12 | 65.9 |
| MS.gene80032.t1 | MTR_2g029400 | 38.824 | 85 | 47 | 1 | 9 | 88 | 14 | 98 | 1.89e-14 | 72.8 |
| MS.gene80032.t1 | MTR_7g066430 | 26.027 | 219 | 111 | 6 | 8 | 221 | 3 | 175 | 2.62e-14 | 72.4 |
| MS.gene80032.t1 | MTR_4g017830 | 27.237 | 257 | 126 | 10 | 10 | 254 | 13 | 220 | 3.20e-14 | 72.0 |
| MS.gene80032.t1 | MTR_4g017830 | 42.169 | 83 | 46 | 1 | 5 | 87 | 263 | 343 | 1.47e-13 | 70.1 |
| MS.gene80032.t1 | MTR_4g017470 | 41.667 | 84 | 47 | 1 | 4 | 87 | 229 | 310 | 3.67e-14 | 72.0 |
| MS.gene80032.t1 | MTR_4g017470 | 32.110 | 109 | 59 | 1 | 7 | 100 | 10 | 118 | 4.22e-11 | 62.8 |
| MS.gene80032.t1 | MTR_4g017610 | 26.459 | 257 | 126 | 9 | 12 | 254 | 16 | 223 | 2.70e-13 | 69.3 |
| MS.gene80032.t1 | MTR_7g063820 | 28.270 | 237 | 116 | 10 | 2 | 232 | 3 | 191 | 3.45e-13 | 68.9 |
| MS.gene80032.t1 | MTR_7g062300 | 25.097 | 259 | 139 | 8 | 2 | 254 | 5 | 214 | 7.10e-13 | 68.2 |
| MS.gene80032.t1 | MTR_7g066410 | 25.581 | 258 | 143 | 9 | 1 | 253 | 276 | 489 | 8.02e-13 | 68.2 |
| MS.gene80032.t1 | MTR_7g066410 | 27.523 | 218 | 109 | 10 | 9 | 221 | 10 | 183 | 4.76e-12 | 65.9 |
| MS.gene80032.t1 | MTR_7g066310 | 33.333 | 117 | 65 | 3 | 9 | 118 | 7 | 117 | 1.03e-12 | 67.4 |
| MS.gene80032.t1 | MTR_7g066360 | 21.790 | 257 | 147 | 5 | 3 | 254 | 8 | 215 | 1.33e-12 | 67.4 |
| MS.gene80032.t1 | MTR_4g017550 | 39.759 | 83 | 42 | 1 | 5 | 87 | 243 | 317 | 1.35e-12 | 67.4 |
| MS.gene80032.t1 | MTR_7g063830 | 32.450 | 151 | 86 | 6 | 1 | 139 | 1 | 147 | 1.62e-12 | 67.0 |
| MS.gene80032.t1 | MTR_7g066580 | 32.743 | 113 | 64 | 4 | 15 | 121 | 1 | 107 | 2.17e-12 | 66.6 |
| MS.gene80032.t1 | MTR_4g017440 | 39.286 | 84 | 49 | 1 | 4 | 87 | 269 | 350 | 3.82e-12 | 65.9 |
| MS.gene80032.t1 | MTR_4g017440 | 23.333 | 270 | 138 | 8 | 3 | 254 | 8 | 226 | 5.00e-12 | 65.9 |
| MS.gene80032.t1 | MTR_7g066490 | 40.909 | 88 | 47 | 1 | 5 | 87 | 12 | 99 | 6.02e-12 | 65.1 |
| MS.gene80032.t1 | MTR_7g066480 | 30.688 | 189 | 113 | 8 | 9 | 188 | 16 | 195 | 1.39e-11 | 64.3 |
| MS.gene80032.t1 | MTR_7g063920 | 38.298 | 94 | 51 | 2 | 1 | 87 | 1 | 94 | 2.16e-11 | 63.5 |
| MS.gene80032.t1 | MTR_3g007740 | 25.490 | 255 | 112 | 8 | 9 | 249 | 9 | 199 | 2.60e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80032.t1 | AT3G09110 | 33.523 | 176 | 95 | 5 | 1 | 163 | 1 | 167 | 1.64e-19 | 86.7 |
| MS.gene80032.t1 | AT5G01130 | 27.308 | 260 | 128 | 6 | 1 | 254 | 1 | 205 | 5.96e-18 | 83.2 |
| MS.gene80032.t1 | AT3G09140 | 25.379 | 264 | 151 | 6 | 1 | 254 | 1 | 228 | 1.06e-16 | 79.3 |
| MS.gene80032.t1 | AT3G09140 | 25.379 | 264 | 151 | 6 | 1 | 254 | 1 | 228 | 1.15e-16 | 79.3 |
| MS.gene80032.t1 | AT5G01120 | 24.812 | 266 | 139 | 7 | 1 | 254 | 1 | 217 | 2.49e-16 | 78.6 |
| MS.gene80032.t1 | AT5G43240 | 25.468 | 267 | 139 | 8 | 1 | 254 | 1 | 220 | 4.13e-15 | 74.7 |
| MS.gene80032.t1 | AT5G43240 | 25.468 | 267 | 139 | 8 | 1 | 254 | 1 | 220 | 4.13e-15 | 74.7 |
| MS.gene80032.t1 | AT5G43240 | 25.468 | 267 | 139 | 8 | 1 | 254 | 1 | 220 | 4.13e-15 | 74.7 |
| MS.gene80032.t1 | AT5G01150 | 23.954 | 263 | 141 | 5 | 1 | 254 | 1 | 213 | 2.82e-14 | 72.4 |
| MS.gene80032.t1 | AT3G09120 | 32.624 | 141 | 83 | 5 | 5 | 138 | 2 | 137 | 5.50e-11 | 62.0 |
Find 0 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GAAAGATGTTATCACATTTA+TGG | - | 23514:28421-28440 | MS.gene80032:CDS | 25.0% |
| ATCATCAATGTCTTCATTCT+GGG | + | 23514:28111-28130 | MS.gene80032:intergenic | 30.0% | |
| TGTTATCACATTTATGGTGA+TGG | - | 23514:28427-28446 | MS.gene80032:CDS | 30.0% | |
| !! | ATTTATGGTGATGGATGATT+TGG | - | 23514:28436-28455 | MS.gene80032:CDS | 30.0% |
| AACAGTGAAGTTCGTTATGT+CGG | - | 23514:28347-28366 | MS.gene80032:CDS | 35.0% | |
| AAGAAGTTTCAGTGACACTT+TGG | + | 23514:27832-27851 | MS.gene80032:intergenic | 35.0% | |
| AAGTTCGTTATGTCGGAAAA+AGG | - | 23514:28354-28373 | MS.gene80032:CDS | 35.0% | |
| ACTAAGTTATACATGTGTCC+AGG | - | 23514:28143-28162 | MS.gene80032:CDS | 35.0% | |
| AGAGTTTCCGATATTAAGAG+TGG | - | 23514:28392-28411 | MS.gene80032:CDS | 35.0% | |
| ATGCTTGATATGGTTGACAT+TGG | + | 23514:28470-28489 | MS.gene80032:intergenic | 35.0% | |
| CAAGAAAAGGTTGTTGAGAT+GGG | - | 23514:28533-28552 | MS.gene80032:CDS | 35.0% | |
| CACAAACCCACTCTTAATAT+CGG | + | 23514:28402-28421 | MS.gene80032:intergenic | 35.0% | |
| CATCATCAATGTCTTCATTC+TGG | + | 23514:28112-28131 | MS.gene80032:intergenic | 35.0% | |
| GAGTTTCCGATATTAAGAGT+GGG | - | 23514:28393-28412 | MS.gene80032:CDS | 35.0% | |
| GATTGAAACCTTGCAAGAAA+AGG | - | 23514:28520-28539 | MS.gene80032:CDS | 35.0% | |
| !! | AATGGAATGGTTGGTAGTTT+AGG | - | 23514:27966-27985 | MS.gene80032:CDS | 35.0% |
| !! | AATTTGCTATGCTTGCCAAT+TGG | - | 23514:27918-27937 | MS.gene80032:CDS | 35.0% |
| !! | GACATTGATGATGAAGATGA+AGG | - | 23514:28119-28138 | MS.gene80032:CDS | 35.0% |
| AAAGGTTGTTGAGATGGGAA+TGG | - | 23514:28538-28557 | MS.gene80032:CDS | 40.0% | |
| AAGTTTCAGTGACACTTTGG+TGG | + | 23514:27829-27848 | MS.gene80032:intergenic | 40.0% | |
| ATAGTCTTCGAGCAGTATAC+TGG | + | 23514:28326-28345 | MS.gene80032:intergenic | 40.0% | |
| CAACAGTGTGATGCTTGATA+TGG | + | 23514:28480-28499 | MS.gene80032:intergenic | 40.0% | |
| CAACCATTCCATTTGTGCTT+AGG | + | 23514:27959-27978 | MS.gene80032:intergenic | 40.0% | |
| CAATGTCTTCATTCTGGGTA+AGG | + | 23514:28106-28125 | MS.gene80032:intergenic | 40.0% | |
| CATGCAACCAAACCAAACTA+AGG | - | 23514:28028-28047 | MS.gene80032:CDS | 40.0% | |
| CTAAGCACAAATGGAATGGT+TGG | - | 23514:27957-27976 | MS.gene80032:CDS | 40.0% | |
| GCAAGAAAAGGTTGTTGAGA+TGG | - | 23514:28532-28551 | MS.gene80032:CDS | 40.0% | |
| GTTAAGCTCCTAAGCACAAA+TGG | - | 23514:27948-27967 | MS.gene80032:CDS | 40.0% | |
| TCGTTATGTCGGAAAAAGGA+AGG | - | 23514:28358-28377 | MS.gene80032:CDS | 40.0% | |
| TTAGCAGGACATCCTTAGTT+TGG | + | 23514:28043-28062 | MS.gene80032:intergenic | 40.0% | |
| ! | AGGACATCCTTAGTTTGGTT+TGG | + | 23514:28038-28057 | MS.gene80032:intergenic | 40.0% |
| ! | CTCAACAACCTTTTCTTGCA+AGG | + | 23514:28531-28550 | MS.gene80032:intergenic | 40.0% |
| AAGGAAGCCTGAGATTTCAG+TGG | + | 23514:28087-28106 | MS.gene80032:intergenic | 45.0% | |
| ACCTCAAAGTCACACTTACC+TGG | + | 23514:28164-28183 | MS.gene80032:intergenic | 45.0% | |
| AGCTCTTCCACTGAAATCTC+AGG | - | 23514:28077-28096 | MS.gene80032:CDS | 45.0% | |
| GCTCCTAAGCACAAATGGAA+TGG | - | 23514:27953-27972 | MS.gene80032:CDS | 45.0% | |
| TCCAGGTAAGTGTGACTTTG+AGG | - | 23514:28160-28179 | MS.gene80032:CDS | 45.0% | |
| TGTGCTTAGGAGCTTAACCA+CGG | + | 23514:27946-27965 | MS.gene80032:intergenic | 45.0% | |
| TGTTGAGATGGGAATGGATG+AGG | - | 23514:28544-28563 | MS.gene80032:CDS | 45.0% | |
| TTTGAGCTCTTGGGTTTAGC+AGG | + | 23514:28058-28077 | MS.gene80032:intergenic | 45.0% | |
| ! | ACCGTGGTTCTCTTCCTTTT+GGG | - | 23514:28228-28247 | MS.gene80032:CDS | 45.0% |
| ! | GGTTTGGTTGCATGTAACTC+TGG | + | 23514:28022-28041 | MS.gene80032:intergenic | 45.0% |
| !! | TGGAAGAGCTTTGAGCTCTT+GGG | + | 23514:28067-28086 | MS.gene80032:intergenic | 45.0% |
| AGGAAGAGAACCACGGTCAT+TGG | + | 23514:28225-28244 | MS.gene80032:intergenic | 50.0% | |
| CCCCAAAAGGAAGAGAACCA+CGG | + | 23514:28232-28251 | MS.gene80032:intergenic | 50.0% | |
| ! | AGCTTAACCACGGTGCCAAT+TGG | + | 23514:27936-27955 | MS.gene80032:intergenic | 50.0% |
| ! | CCGTGGTTCTCTTCCTTTTG+GGG | - | 23514:28229-28248 | MS.gene80032:CDS | 50.0% |
| ! | GACCGTGGTTCTCTTCCTTT+TGG | - | 23514:28227-28246 | MS.gene80032:CDS | 50.0% |
| !! | AAAGCAGGACTAGCCCCAAA+AGG | + | 23514:28245-28264 | MS.gene80032:intergenic | 50.0% |
| !! | GTGGAAGAGCTTTGAGCTCT+TGG | + | 23514:28068-28087 | MS.gene80032:intergenic | 50.0% |
| !!! | CTGCTTTTGGGGCTGCTTTT+GGG | - | 23514:28276-28295 | MS.gene80032:CDS | 50.0% |
| !!! | TGCTTTTGGGGCTGCTTTTG+GGG | - | 23514:28277-28296 | MS.gene80032:CDS | 50.0% |
| !!! | TTGGGGCTAGTCCTGCTTTT+GGG | - | 23514:28246-28265 | MS.gene80032:CDS | 50.0% |
| !!! | TTTGGGGCTAGTCCTGCTTT+TGG | - | 23514:28245-28264 | MS.gene80032:CDS | 50.0% |
| ACACCACTCGTTGTACTGCC+AGG | - | 23514:28195-28214 | MS.gene80032:CDS | 55.0% | |
| AGACCTGGCAGTACAACGAG+TGG | + | 23514:28201-28220 | MS.gene80032:intergenic | 55.0% | |
| ATGCTTGCCAATTGGCACCG+TGG | - | 23514:27926-27945 | MS.gene80032:CDS | 55.0% | |
| CCAAAAGCAGCCCCAAAAGC+AGG | + | 23514:28278-28297 | MS.gene80032:intergenic | 55.0% | |
| !! | ACTGGACTAGCCCCAAAAGC+AGG | + | 23514:28308-28327 | MS.gene80032:intergenic | 55.0% |
| !!! | CCTGCTTTTGGGGCTGCTTT+TGG | - | 23514:28275-28294 | MS.gene80032:CDS | 55.0% |
| !!! | TGGGGCTAGTCCTGCTTTTG+GGG | - | 23514:28247-28266 | MS.gene80032:CDS | 55.0% |
| ACCACGGTCATTGGCAGACC+TGG | + | 23514:28216-28235 | MS.gene80032:intergenic | 60.0% | |
| !! | GCAGGACTAGCCCCAAAAGC+AGG | + | 23514:28260-28279 | MS.gene80032:intergenic | 60.0% |
| GCCAGGTCTGCCAATGACCG+TGG | - | 23514:28212-28231 | MS.gene80032:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 23514 | gene | 27807 | 28574 | 27807 | ID=MS.gene80032 |
| 23514 | mRNA | 27807 | 28574 | 27807 | ID=MS.gene80032.t1;Parent=MS.gene80032 |
| 23514 | exon | 27807 | 28574 | 27807 | ID=MS.gene80032.t1.exon1;Parent=MS.gene80032.t1 |
| 23514 | CDS | 27807 | 28574 | 27807 | ID=cds.MS.gene80032.t1;Parent=MS.gene80032.t1 |
| Gene Sequence |
| Protein sequence |
