Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80101.t1 | XP_013457391.1 | 100 | 57 | 0 | 0 | 4 | 60 | 1 | 57 | 4.40E-24 | 119.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80101.t1 | G8A1V0 | 100.0 | 57 | 0 | 0 | 4 | 60 | 1 | 57 | 3.2e-24 | 119.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054937 | MS.gene80101 | 0.804031 | 2.63E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 8 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTTCAAGTTGTAGGATAA+TGG | 0.313664 | 8.4:+69122438 | None:intergenic |
| ATTTCAAGTTGTAGGATAAT+GGG | 0.372893 | 8.4:+69122439 | None:intergenic |
| AGGATAATGGGGCGGAAAGC+TGG | 0.409342 | 8.4:+69122451 | MS.gene80101:CDS |
| AAATACATGTATAGAATCTC+AGG | 0.487514 | 8.4:+69122606 | MS.gene80101:CDS |
| TAATGGGGCGGAAAGCTGGC+GGG | 0.509687 | 8.4:+69122455 | MS.gene80101:CDS |
| ACTTCATAAACCTTGCATGA+AGG | 0.526107 | 8.4:+69122507 | MS.gene80101:CDS |
| GATCATGTTTATGAACTGTA+TGG | 0.548690 | 8.4:+69122534 | MS.gene80101:CDS |
| ATGATCATTTCCTTCATGCA+AGG | 0.553694 | 8.4:-69122517 | None:intergenic |
| ATAATGGGGCGGAAAGCTGG+CGG | 0.563977 | 8.4:+69122454 | MS.gene80101:CDS |
| TGAAGCATGTGCTAAGCAGA+AGG | 0.631587 | 8.4:+69122576 | MS.gene80101:CDS |
| TTTCAAGTTGTAGGATAATG+GGG | 0.640667 | 8.4:+69122440 | None:intergenic |
| CAAGTTGTAGGATAATGGGG+CGG | 0.699621 | 8.4:+69122443 | None:intergenic |
| CACATGCTTCAACATCACTG+TGG | 0.742984 | 8.4:-69122564 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GATCATGTTTATGAACTGTA+TGG | + | chr8.4:69122534-69122553 | MS.gene80101:CDS | 30.0% | |
| ACTTCATAAACCTTGCATGA+AGG | + | chr8.4:69122507-69122526 | MS.gene80101:CDS | 35.0% | |
| ATGATCATTTCCTTCATGCA+AGG | - | chr8.4:69122520-69122539 | None:intergenic | 35.0% | |
| CACATGCTTCAACATCACTG+TGG | - | chr8.4:69122567-69122586 | None:intergenic | 45.0% | |
| TGAAGCATGTGCTAAGCAGA+AGG | + | chr8.4:69122576-69122595 | MS.gene80101:CDS | 45.0% | |
| ! | ATAATGGGGCGGAAAGCTGG+CGG | + | chr8.4:69122454-69122473 | MS.gene80101:CDS | 55.0% |
| !! | AGGATAATGGGGCGGAAAGC+TGG | + | chr8.4:69122451-69122470 | MS.gene80101:CDS | 55.0% |
| TAATGGGGCGGAAAGCTGGC+GGG | + | chr8.4:69122455-69122474 | MS.gene80101:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 69122447 | 69122627 | 69122447 | ID=MS.gene80101 |
| chr8.4 | mRNA | 69122447 | 69122627 | 69122447 | ID=MS.gene80101.t1;Parent=MS.gene80101 |
| chr8.4 | exon | 69122447 | 69122627 | 69122447 | ID=MS.gene80101.t1.exon1;Parent=MS.gene80101.t1 |
| chr8.4 | CDS | 69122447 | 69122627 | 69122447 | ID=cds.MS.gene80101.t1;Parent=MS.gene80101.t1 |
| Gene Sequence |
| Protein sequence |
