Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80188.t1 | AES73545.2 | 98.9 | 178 | 2 | 0 | 1 | 178 | 277 | 454 | 2.30E-97 | 364.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80188.t1 | Q54SA1 | 31.9 | 166 | 88 | 3 | 1 | 166 | 279 | 419 | 8.8e-21 | 101.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80188.t1 | A0A072VCN8 | 98.9 | 178 | 2 | 0 | 1 | 178 | 333 | 510 | 1.7e-97 | 364.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049825 | MS.gene80188 | 0.804154 | 2.48E-49 | -1.69E-46 |
| MS.gene049873 | MS.gene80188 | 0.8017 | 7.96E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80188.t1 | MTR_3g105980 | 98.876 | 178 | 2 | 0 | 1 | 178 | 277 | 454 | 3.34e-127 | 365 |
| MS.gene80188.t1 | MTR_3g105980 | 98.876 | 178 | 2 | 0 | 1 | 178 | 333 | 510 | 1.71e-126 | 365 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCAGAGCATTTGTTGTATT+TGG | 0.114765 | 3.4:-92456749 | None:intergenic |
| TTGGGACAAGCAACCTTGTT+TGG | 0.271840 | 3.4:+92456917 | MS.gene80188:CDS |
| TAAAGATATTGGTGGCATAT+TGG | 0.305454 | 3.4:+92456656 | MS.gene80188:CDS |
| AAAGATATTGGTGGCATATT+GGG | 0.328535 | 3.4:+92456657 | MS.gene80188:CDS |
| AGCAGAGATTGTTGGTATAG+AGG | 0.338881 | 3.4:-92456720 | None:intergenic |
| TGATTCACTCTTGTAAAATC+AGG | 0.342748 | 3.4:-92456859 | None:intergenic |
| TATCTTTACTGTTGCATGCT+TGG | 0.359543 | 3.4:-92456641 | None:intergenic |
| TCCAAATACAACAAATGCTC+TGG | 0.360734 | 3.4:+92456748 | MS.gene80188:CDS |
| TTACAGCAGGTGTTAGCTTT+GGG | 0.376299 | 3.4:+92456953 | MS.gene80188:CDS |
| GTTACAGCAGGTGTTAGCTT+TGG | 0.378734 | 3.4:+92456952 | MS.gene80188:CDS |
| TGGGACAAGCAACCTTGTTT+GGG | 0.391086 | 3.4:+92456918 | MS.gene80188:CDS |
| ACAGGACCTGCCATTCATAA+TGG | 0.397851 | 3.4:+92456814 | MS.gene80188:CDS |
| AATATGCAGTTAGTAATGTA+AGG | 0.411467 | 3.4:+92456887 | MS.gene80188:CDS |
| TGGTATAGAGGAGATACTTC+AGG | 0.432932 | 3.4:-92456708 | None:intergenic |
| GGAAGAAGAGCAGAGATTGT+TGG | 0.437012 | 3.4:-92456728 | None:intergenic |
| TGCCATTGTATCTCAACTTA+AGG | 0.439601 | 3.4:+92456987 | MS.gene80188:CDS |
| GAGATGACCAGAAAATTGTT+TGG | 0.457256 | 3.4:-92456254 | None:intergenic |
| GCATGCAACAGTAAAGATAT+TGG | 0.462232 | 3.4:+92456645 | MS.gene80188:CDS |
| TGTACTACTTCCATTATGAA+TGG | 0.463453 | 3.4:-92456824 | None:intergenic |
| ACTCTCTCTAGCTCTTGAAT+AGG | 0.468910 | 3.4:-92457048 | None:intergenic |
| GACTATTTCTATGTTACAGC+AGG | 0.481970 | 3.4:+92456940 | MS.gene80188:CDS |
| ATTCATAATGGAAGTAGTAC+AGG | 0.489868 | 3.4:+92456826 | MS.gene80188:CDS |
| CTACTTCCATTATGAATGGC+AGG | 0.510980 | 3.4:-92456820 | None:intergenic |
| GTAATGTAAGGGCACACATT+GGG | 0.512078 | 3.4:+92456899 | MS.gene80188:CDS |
| GTACCAGGTTTCAACAATAC+AGG | 0.528175 | 3.4:+92456796 | MS.gene80188:CDS |
| ATCAAATATTATGTGGTACC+AGG | 0.539257 | 3.4:+92456781 | MS.gene80188:CDS |
| CCAAATACAACAAATGCTCT+GGG | 0.539667 | 3.4:+92456749 | MS.gene80188:CDS |
| TTCCTTAAGTTGAGATACAA+TGG | 0.539766 | 3.4:-92456989 | None:intergenic |
| GATTCACTCTTGTAAAATCA+GGG | 0.566765 | 3.4:-92456858 | None:intergenic |
| TCTTGAATAGGAACAGCATA+GGG | 0.569621 | 3.4:-92457036 | None:intergenic |
| CTCTTGAATAGGAACAGCAT+AGG | 0.588342 | 3.4:-92457037 | None:intergenic |
| TGACTGACCTAGCCAGTCCA+TGG | 0.592890 | 3.4:-92456220 | None:intergenic |
| GGTCCTGTATTGTTGAAACC+TGG | 0.596755 | 3.4:-92456799 | None:intergenic |
| AGTTGAGATCAAATATTATG+TGG | 0.603655 | 3.4:+92456774 | MS.gene80188:CDS |
| AGTAATGTAAGGGCACACAT+TGG | 0.611816 | 3.4:+92456898 | MS.gene80188:CDS |
| TTAGAATCAATACCATGGAC+TGG | 0.612694 | 3.4:+92456208 | None:intergenic |
| ATATGCAGTTAGTAATGTAA+GGG | 0.626582 | 3.4:+92456888 | MS.gene80188:CDS |
| CATAGAAATAGTCCCAAACA+AGG | 0.630129 | 3.4:-92456930 | None:intergenic |
| ATCAATACCATGGACTGGCT+AGG | 0.630406 | 3.4:+92456213 | None:intergenic |
| TGCAACAGTAAAGATATTGG+TGG | 0.631511 | 3.4:+92456648 | MS.gene80188:CDS |
| TCTGTCTTCTGCAATATCAG+AGG | 0.645205 | 3.4:+92456275 | MS.gene80188:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAAAAATTTATACTATCT+AGG | + | chr3.4:92456571-92456590 | MS.gene80188:intron | 10.0% |
| !! | ATAAAAATTTATACTATCTA+GGG | + | chr3.4:92456572-92456591 | MS.gene80188:intron | 10.0% |
| !!! | AGTTAATAAGTCATAATTTT+CGG | - | chr3.4:92456510-92456529 | None:intergenic | 15.0% |
| ! | AATATGCAGTTAGTAATGTA+AGG | + | chr3.4:92456887-92456906 | MS.gene80188:CDS | 25.0% |
| ! | AGTTGAGATCAAATATTATG+TGG | + | chr3.4:92456774-92456793 | MS.gene80188:CDS | 25.0% |
| ! | ATATGCAGTTAGTAATGTAA+GGG | + | chr3.4:92456888-92456907 | MS.gene80188:CDS | 25.0% |
| !! | ATATTCTTTGTTGTCTTTCA+TGG | + | chr3.4:92456327-92456346 | MS.gene80188:intron | 25.0% |
| !! | ATTTTCTTACGATGTTTCAA+CGG | + | chr3.4:92456599-92456618 | MS.gene80188:intron | 25.0% |
| AATATACAATGTCAGTGTGA+AGG | - | chr3.4:92456543-92456562 | None:intergenic | 30.0% | |
| ATCAAATATTATGTGGTACC+AGG | + | chr3.4:92456781-92456800 | MS.gene80188:CDS | 30.0% | |
| GATTCACTCTTGTAAAATCA+GGG | - | chr3.4:92456861-92456880 | None:intergenic | 30.0% | |
| TGATTCACTCTTGTAAAATC+AGG | - | chr3.4:92456862-92456881 | None:intergenic | 30.0% | |
| TGTACTACTTCCATTATGAA+TGG | - | chr3.4:92456827-92456846 | None:intergenic | 30.0% | |
| ! | AAAGATATTGGTGGCATATT+GGG | + | chr3.4:92456657-92456676 | MS.gene80188:CDS | 30.0% |
| ! | ATTCATAATGGAAGTAGTAC+AGG | + | chr3.4:92456826-92456845 | MS.gene80188:CDS | 30.0% |
| ! | GATTTTACAAGAGTGAATCA+TGG | + | chr3.4:92456862-92456881 | MS.gene80188:CDS | 30.0% |
| ! | TAAAGATATTGGTGGCATAT+TGG | + | chr3.4:92456656-92456675 | MS.gene80188:CDS | 30.0% |
| ! | TTACAGACCAAACAATTTTC+TGG | + | chr3.4:92456247-92456266 | MS.gene80188:CDS | 30.0% |
| !! | TAGTTCTAAAGCCATGTATA+TGG | - | chr3.4:92456456-92456475 | None:intergenic | 30.0% |
| !! | TTCCTTAAGTTGAGATACAA+TGG | - | chr3.4:92456992-92457011 | None:intergenic | 30.0% |
| ATCACGACAAACCATATACA+TGG | + | chr3.4:92456442-92456461 | MS.gene80188:intron | 35.0% | |
| CATAGAAATAGTCCCAAACA+AGG | - | chr3.4:92456933-92456952 | None:intergenic | 35.0% | |
| CCAAATACAACAAATGCTCT+GGG | + | chr3.4:92456749-92456768 | MS.gene80188:CDS | 35.0% | |
| GAGATGACCAGAAAATTGTT+TGG | - | chr3.4:92456257-92456276 | None:intergenic | 35.0% | |
| GCATGCAACAGTAAAGATAT+TGG | + | chr3.4:92456645-92456664 | MS.gene80188:CDS | 35.0% | |
| GGTTTGTCGTGATTTATTGA+CGG | - | chr3.4:92456435-92456454 | None:intergenic | 35.0% | |
| TATCTTTACTGTTGCATGCT+TGG | - | chr3.4:92456644-92456663 | None:intergenic | 35.0% | |
| TCCAAATACAACAAATGCTC+TGG | + | chr3.4:92456748-92456767 | MS.gene80188:CDS | 35.0% | |
| TCTTGAATAGGAACAGCATA+GGG | - | chr3.4:92457039-92457058 | None:intergenic | 35.0% | |
| TGCAACAGTAAAGATATTGG+TGG | + | chr3.4:92456648-92456667 | MS.gene80188:CDS | 35.0% | |
| TGCCATTGTATCTCAACTTA+AGG | + | chr3.4:92456987-92457006 | MS.gene80188:CDS | 35.0% | |
| ! | AGGAAATTTTTGATGCTGAC+TGG | + | chr3.4:92457007-92457026 | MS.gene80188:CDS | 35.0% |
| !! | GACTATTTCTATGTTACAGC+AGG | + | chr3.4:92456940-92456959 | MS.gene80188:CDS | 35.0% |
| AGCAGAGATTGTTGGTATAG+AGG | - | chr3.4:92456723-92456742 | None:intergenic | 40.0% | |
| CTACTTCCATTATGAATGGC+AGG | - | chr3.4:92456823-92456842 | None:intergenic | 40.0% | |
| CTCTTGAATAGGAACAGCAT+AGG | - | chr3.4:92457040-92457059 | None:intergenic | 40.0% | |
| GATGTTTCAACGGAACAAAC+AGG | + | chr3.4:92456609-92456628 | MS.gene80188:intron | 40.0% | |
| GTACCAGGTTTCAACAATAC+AGG | + | chr3.4:92456796-92456815 | MS.gene80188:CDS | 40.0% | |
| TCTGTCTTCTGCAATATCAG+AGG | + | chr3.4:92456275-92456294 | MS.gene80188:CDS | 40.0% | |
| TGGTATAGAGGAGATACTTC+AGG | - | chr3.4:92456711-92456730 | None:intergenic | 40.0% | |
| ! | AGTAATGTAAGGGCACACAT+TGG | + | chr3.4:92456898-92456917 | MS.gene80188:CDS | 40.0% |
| ! | GTAATGTAAGGGCACACATT+GGG | + | chr3.4:92456899-92456918 | MS.gene80188:CDS | 40.0% |
| !! | ACTCTCTCTAGCTCTTGAAT+AGG | - | chr3.4:92457051-92457070 | None:intergenic | 40.0% |
| !! | CCCAGAGCATTTGTTGTATT+TGG | - | chr3.4:92456752-92456771 | None:intergenic | 40.0% |
| !! | TTACAGCAGGTGTTAGCTTT+GGG | + | chr3.4:92456953-92456972 | MS.gene80188:CDS | 40.0% |
| ACAGGACCTGCCATTCATAA+TGG | + | chr3.4:92456814-92456833 | MS.gene80188:CDS | 45.0% | |
| GGAAGAAGAGCAGAGATTGT+TGG | - | chr3.4:92456731-92456750 | None:intergenic | 45.0% | |
| GGTCCTGTATTGTTGAAACC+TGG | - | chr3.4:92456802-92456821 | None:intergenic | 45.0% | |
| TTGGGACAAGCAACCTTGTT+TGG | + | chr3.4:92456917-92456936 | MS.gene80188:CDS | 45.0% | |
| ! | GTTACAGCAGGTGTTAGCTT+TGG | + | chr3.4:92456952-92456971 | MS.gene80188:CDS | 45.0% |
| ! | TGGGACAAGCAACCTTGTTT+GGG | + | chr3.4:92456918-92456937 | MS.gene80188:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 92456222 | 92457092 | 92456222 | ID=MS.gene80188 |
| chr3.4 | mRNA | 92456222 | 92457092 | 92456222 | ID=MS.gene80188.t1;Parent=MS.gene80188 |
| chr3.4 | exon | 92456222 | 92456296 | 92456222 | ID=MS.gene80188.t1.exon1;Parent=MS.gene80188.t1 |
| chr3.4 | CDS | 92456222 | 92456296 | 92456222 | ID=cds.MS.gene80188.t1;Parent=MS.gene80188.t1 |
| chr3.4 | exon | 92456631 | 92457092 | 92456631 | ID=MS.gene80188.t1.exon2;Parent=MS.gene80188.t1 |
| chr3.4 | CDS | 92456631 | 92457092 | 92456631 | ID=cds.MS.gene80188.t1;Parent=MS.gene80188.t1 |
| Gene Sequence |
| Protein sequence |
