AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene80190

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80190.t1 MTR_3g106000 94.891 274 14 0 2 275 1 274 0.0 541
MS.gene80190.t1 MTR_1g013820 61.511 278 100 4 1 275 1 274 1.92e-123 353
MS.gene80190.t1 MTR_1g013820 65.737 251 83 2 28 275 4 254 8.55e-123 350
MS.gene80190.t1 MTR_1g013820 65.339 251 82 3 28 275 4 252 2.72e-120 344
MS.gene80190.t1 MTR_6g013425 45.818 275 142 4 5 275 45 316 2.64e-78 240
MS.gene80190.t1 MTR_5g043210 49.393 247 120 4 33 275 18 263 2.85e-76 233
MS.gene80190.t1 MTR_5g043210 47.909 263 131 5 18 275 57 318 2.01e-75 233
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80190.t1 AT5G10870 57.588 257 104 2 24 275 9 265 2.04e-99 292
MS.gene80190.t1 AT1G69370 48.810 252 124 3 29 275 65 316 7.76e-79 241
MS.gene80190.t1 AT3G29200 45.290 276 139 3 11 275 66 340 1.49e-76 236

Find 50 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGAAGCAGCTTCAATTTA+TGG 0.102058 3.4:-92471247 MS.gene80190:CDS
TATACCTTGGCACGAATTAA+AGG 0.253511 3.4:+92468228 None:intergenic
GGTGGTAAAATTCCTTTATA+AGG 0.277992 3.4:+92469476 None:intergenic
AAGATGCCCTCACTGATTCT+AGG 0.300800 3.4:+92471151 None:intergenic
AGCAACTACCTTGGCTTGAA+TGG 0.341924 3.4:+92470984 None:intergenic
ATCTCTAGAAGGATTCATTA+TGG 0.362754 3.4:-92468300 MS.gene80190:CDS
CCTGAAGAAAATCCTTATAA+AGG 0.368752 3.4:-92469488 MS.gene80190:CDS
GAAAAGAAGGCCATGGTGTT+TGG 0.389912 3.4:-92467976 MS.gene80190:CDS
AACGACATCGAGCAACGAAC+CGG 0.410241 3.4:+92471023 None:intergenic
TACAAGCGAGATACTATTGC+TGG 0.411415 3.4:+92467904 None:intergenic
TTTGTTGCTTTCGGGGATGA+TGG 0.428043 3.4:-92468596 MS.gene80190:CDS
AGAAGAGATAGTTGTGAAGA+GGG 0.446517 3.4:-92468001 MS.gene80190:CDS
TAGAAGAGATAGTTGTGAAG+AGG 0.455201 3.4:-92468002 MS.gene80190:CDS
TGGTAGGTGGGAGAATTAAG+AGG 0.466502 3.4:+92471075 None:intergenic
TGTTATGCTGTTAAGTTGTC+CGG 0.466573 3.4:-92471217 MS.gene80190:CDS
ATTCGCAGGCCATCTCTAGA+AGG 0.470601 3.4:-92468311 MS.gene80190:intron
TGTGAAGAGGGTTGAAAAGA+AGG 0.489246 3.4:-92467989 MS.gene80190:CDS
GCAGTTGATCTGTGTCTCTC+CGG 0.489387 3.4:+92471198 None:intergenic
TTAACATAAACAAGAACATA+AGG 0.494659 3.4:-92468649 MS.gene80190:CDS
AGACACAGATCAACTGCAAA+TGG 0.502992 3.4:-92471192 MS.gene80190:CDS
GTCCCTTGACATCATGATCA+AGG 0.517248 3.4:+92467941 None:intergenic
GGTAGGTGGGAGAATTAAGA+GGG 0.519339 3.4:+92471076 None:intergenic
GCAGCTTCAATTTATGGCTG+TGG 0.525817 3.4:-92471241 MS.gene80190:CDS
CTTGTTTATGTTAATCGAAG+CGG 0.533853 3.4:+92468658 None:intergenic
AATAGTATCTCGCTTGTACA+AGG 0.537580 3.4:-92467899 MS.gene80190:CDS
TTGTAATGTGGCACAATTGA+GGG 0.543976 3.4:+92469455 None:intergenic
TATCTCGCTTGTACAAGGAA+TGG 0.544751 3.4:-92467894 MS.gene80190:CDS
AGATGCCCTCACTGATTCTA+GGG 0.546079 3.4:+92471152 None:intergenic
GAATGGAAGCATAATGTTGA+TGG 0.547788 3.4:+92471055 None:intergenic
TGATGTCAAGGGACATTACA+AGG 0.549802 3.4:-92467932 MS.gene80190:CDS
GCATAATGTTGATGGTAGGT+GGG 0.554786 3.4:+92471063 None:intergenic
TTATGGAAAGTTTGTAGCTG+AGG 0.563769 3.4:-92468283 MS.gene80190:CDS
GAGCCTTGATCATGATGTCA+AGG 0.568630 3.4:-92467944 MS.gene80190:CDS
ATAATGAATCCTTCTAGAGA+TGG 0.570026 3.4:+92468302 None:intergenic
TGAGCCTTTAATTCGTGCCA+AGG 0.575950 3.4:-92468232 MS.gene80190:intron
AGCATAATGTTGATGGTAGG+TGG 0.585994 3.4:+92471062 None:intergenic
TACTGAAGCCATTCAAGCCA+AGG 0.587805 3.4:-92470992 MS.gene80190:intron
CGAACCGGAAAAGTGAGGAA+TGG 0.610407 3.4:+92471038 None:intergenic
GCCACATTACAACTTCTCAC+AGG 0.612065 3.4:-92469444 MS.gene80190:intron
GGAAGCATAATGTTGATGGT+AGG 0.616006 3.4:+92471059 None:intergenic
AGCAACGAACCGGAAAAGTG+AGG 0.623308 3.4:+92471033 None:intergenic
GAGGGTTGAAAAGAAGGCCA+TGG 0.631982 3.4:-92467983 MS.gene80190:CDS
CACTTCTTGACCAAACACCA+TGG 0.632219 3.4:+92467966 None:intergenic
AGCCTTGATCATGATGTCAA+GGG 0.643010 3.4:-92467943 MS.gene80190:CDS
GTTGTAATGTGGCACAATTG+AGG 0.646000 3.4:+92469454 None:intergenic
ACCTGTGAGAAGTTGTAATG+TGG 0.704606 3.4:+92469443 None:intergenic
AATACACCCTAGAATCAGTG+AGG 0.710690 3.4:-92471158 MS.gene80190:CDS
ATACACCCTAGAATCAGTGA+GGG 0.715519 3.4:-92471157 MS.gene80190:CDS
TAATGTGGCACAATTGAGGG+TGG 0.727705 3.4:+92469458 None:intergenic
ATTAAAGGCTCATAGTCACG+AGG 0.752224 3.4:+92468243 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTTAATACAGAAAATTT+AGG + chr3.4:92469487-92469506 None:intergenic 10.0%
!!! ATTTTCTGTATTAAATAAAT+TGG - chr3.4:92469489-92469508 MS.gene80190:CDS 10.0%
!!! ATTTTGTATTAGAATTTATT+GGG - chr3.4:92468168-92468187 MS.gene80190:intron 10.0%
!!! ATTTTTCTAAAAATAATCTT+TGG - chr3.4:92469894-92469913 MS.gene80190:intron 10.0%
!!! TAGTTTTAAAAGTAATAATT+TGG + chr3.4:92468942-92468961 None:intergenic 10.0%
!! AAATAAAGCCAAAAATAAAT+AGG + chr3.4:92468668-92468687 None:intergenic 15.0%
!! AAATTATCCTTATTAACTAT+TGG + chr3.4:92468966-92468985 None:intergenic 15.0%
!! AGTTATAGATGATTAAAATA+CGG - chr3.4:92468519-92468538 MS.gene80190:intron 15.0%
!! TTAAGAATCTTAAATACAAT+AGG - chr3.4:92469167-92469186 MS.gene80190:intron 15.0%
!!! ACATTCTTTATTTTTTTTGA+TGG + chr3.4:92468773-92468792 None:intergenic 15.0%
!!! CGAATATATTTTATAGAATA+AGG + chr3.4:92470760-92470779 None:intergenic 15.0%
!!! GATCTAATGATATTTTAAAA+GGG - chr3.4:92468575-92468594 MS.gene80190:CDS 15.0%
!!! GATTTTGTATTAGAATTTAT+TGG - chr3.4:92468167-92468186 MS.gene80190:intron 15.0%
!!! GGTTTTTTTTTTTTTTTGTA+AGG - chr3.4:92468290-92468309 MS.gene80190:CDS 15.0%
!!! TAAGACAATTTTGTTATATT+TGG - chr3.4:92469844-92469863 MS.gene80190:intron 15.0%
!! AAACTAACCAATAGTTAATA+AGG - chr3.4:92468956-92468975 MS.gene80190:intron 20.0%
!! AAATCAAAAAGAAGAAGAAA+AGG - chr3.4:92469982-92470001 MS.gene80190:intron 20.0%
!! AGAAAAAGTTATTATTGCAT+TGG + chr3.4:92468879-92468898 None:intergenic 20.0%
!! ATAAATAAAACAAGATGCAT+AGG + chr3.4:92468367-92468386 None:intergenic 20.0%
!! ATGTAAAAATGAGAAAATCA+AGG + chr3.4:92470689-92470708 None:intergenic 20.0%
!! ATGTTAGTGGAAAATAATAA+AGG - chr3.4:92468553-92468572 MS.gene80190:CDS 20.0%
!! ATTCTATAAAATATATTCGC+AGG - chr3.4:92470761-92470780 MS.gene80190:intron 20.0%
!! CAAAAAAAAAAAAAAACCAG+AGG + chr3.4:92468288-92468307 None:intergenic 20.0%
!! TCAAAATTGTATGAAAATCA+AGG + chr3.4:92469361-92469380 None:intergenic 20.0%
!! TCTATCTTTGTAATATGTAT+AGG + chr3.4:92470036-92470055 None:intergenic 20.0%
!! TCTTATTAACAAGATAAAGA+TGG - chr3.4:92470613-92470632 MS.gene80190:intron 20.0%
!! TGAAATATTATTCGTTAGTT+AGG - chr3.4:92469562-92469581 MS.gene80190:intron 20.0%
!! TTAACATAAACAAGAACATA+AGG - chr3.4:92470437-92470456 MS.gene80190:intron 20.0%
!! TTCTGTATTAAATAAATTGG+TGG - chr3.4:92469492-92469511 MS.gene80190:CDS 20.0%
!! TTTATATCAAAGGAAGATAT+TGG - chr3.4:92470115-92470134 MS.gene80190:intron 20.0%
!!! AAAAATAATCTTTGGAAATG+CGG - chr3.4:92469902-92469921 MS.gene80190:intron 20.0%
!!! AATTTTGCAGGTAAAATTTT+CGG - chr3.4:92470546-92470565 MS.gene80190:intron 20.0%
!!! ATTTCCACGAATAAATTTTA+TGG + chr3.4:92470331-92470350 None:intergenic 20.0%
!!! CATTTTGAAACAAATTGTTT+TGG + chr3.4:92468839-92468858 None:intergenic 20.0%
!!! GGATCTAATGATATTTTAAA+AGG - chr3.4:92468574-92468593 MS.gene80190:CDS 20.0%
!!! TCTCAAAAATATGTTTTACT+GGG + chr3.4:92470069-92470088 None:intergenic 20.0%
!!! TCTTTATTTTTTTTGATGGT+CGG + chr3.4:92468769-92468788 None:intergenic 20.0%
!!! TTCATTAAATTTTCATTTCG+TGG + chr3.4:92469311-92469330 None:intergenic 20.0%
!!! TTCTAATGCTTTTATATCAA+AGG - chr3.4:92470105-92470124 MS.gene80190:intron 20.0%
!!! TTCTCAAAAATATGTTTTAC+TGG + chr3.4:92470070-92470089 None:intergenic 20.0%
!!! TTTGATGGTAGTAAAAATAA+TGG - chr3.4:92469712-92469731 MS.gene80190:intron 20.0%
!!! TTTTGGTGTAAAAATTGAAA+CGG - chr3.4:92471001-92471020 MS.gene80190:CDS 20.0%
! ATAGATGATTAAAATACGGT+AGG - chr3.4:92468523-92468542 MS.gene80190:intron 25.0%
! ATCTTGTTAATAAGAACCAA+GGG + chr3.4:92470608-92470627 None:intergenic 25.0%
! ATGCAACAATAAAGAATGTA+TGG - chr3.4:92468706-92468725 MS.gene80190:intron 25.0%
! CTCTTGAATTGATTAGAAAA+GGG + chr3.4:92470231-92470250 None:intergenic 25.0%
! CTTTCATTAATTCGATTGTA+AGG + chr3.4:92469109-92469128 None:intergenic 25.0%
! GTAAAAATTGAAACGGTAAA+TGG - chr3.4:92471008-92471027 MS.gene80190:CDS 25.0%
! TATCTTGTTAATAAGAACCA+AGG + chr3.4:92470609-92470628 None:intergenic 25.0%
! TCAACCATGAAAAGAAAATA+AGG - chr3.4:92470365-92470384 MS.gene80190:intron 25.0%
! TCCAAAACTCTTCAATTTAT+CGG - chr3.4:92468233-92468252 MS.gene80190:CDS 25.0%
! TCGAATTAATGAAAGAGAAA+TGG - chr3.4:92469114-92469133 MS.gene80190:intron 25.0%
! TCTCTTGAATTGATTAGAAA+AGG + chr3.4:92470232-92470251 None:intergenic 25.0%
! TGTTCCATAAAATTTATTCG+TGG - chr3.4:92470324-92470343 MS.gene80190:intron 25.0%
!! ATTAAGTTGTAATCATGTGA+CGG + chr3.4:92469434-92469453 None:intergenic 25.0%
!! CGTTTAAAACTTAAATGTGA+GGG + chr3.4:92468409-92468428 None:intergenic 25.0%
!! CTCAAAAATATGTTTTACTG+GGG + chr3.4:92470068-92470087 None:intergenic 25.0%
!! CTTTATAAGGATTTTCTTCA+GGG + chr3.4:92469600-92469619 None:intergenic 25.0%
!! TTTATGTTGTTACTACGTTT+TGG - chr3.4:92470984-92471003 MS.gene80190:intron 25.0%
!!! AAGTTCAACATTTTTAGTCA+TGG + chr3.4:92471225-92471244 None:intergenic 25.0%
!!! AGAGGTTACCTATTTATTTT+TGG - chr3.4:92468657-92468676 MS.gene80190:CDS 25.0%
!!! AGATCCTTATTTTCTTTTCA+TGG + chr3.4:92470372-92470391 None:intergenic 25.0%
!!! AGTTCAACATTTTTAGTCAT+GGG + chr3.4:92471224-92471243 None:intergenic 25.0%
!!! CCTCACATTTAAGTTTTAAA+CGG - chr3.4:92468407-92468426 MS.gene80190:intron 25.0%
!!! CGTTTTTTAAATGCAAAACT+TGG - chr3.4:92470958-92470977 MS.gene80190:intron 25.0%
!!! CTTTATTTTTTTTGATGGTC+GGG + chr3.4:92468768-92468787 None:intergenic 25.0%
!!! TTTATTTTTTTTGATGGTCG+GGG + chr3.4:92468767-92468786 None:intergenic 25.0%
AAAAAAAGAGTAGGAAGATG+CGG + chr3.4:92468260-92468279 None:intergenic 30.0%
AAAGGAAGATATTGGAGAAA+AGG - chr3.4:92470123-92470142 MS.gene80190:intron 30.0%
AAGATAAAGATGGATATCGT+AGG - chr3.4:92470623-92470642 MS.gene80190:intron 30.0%
AAGGAAGATATTGGAGAAAA+GGG - chr3.4:92470124-92470143 MS.gene80190:intron 30.0%
CCTGAAGAAAATCCTTATAA+AGG - chr3.4:92469598-92469617 MS.gene80190:intron 30.0%
CTCGATTACAGATAATTCTA+AGG - chr3.4:92470183-92470202 MS.gene80190:intron 30.0%
CTGTGTGATTTGATATTCTA+GGG - chr3.4:92469681-92469700 MS.gene80190:intron 30.0%
CTTAGAATTATCTGTAATCG+AGG + chr3.4:92470185-92470204 None:intergenic 30.0%
CTTGTTTATGTTAATCGAAG+CGG + chr3.4:92470431-92470450 None:intergenic 30.0%
GAATTGATTAGAAAAGGGTT+TGG + chr3.4:92470226-92470245 None:intergenic 30.0%
GAGGATAAGAAAAAAAGAGT+AGG + chr3.4:92468269-92468288 None:intergenic 30.0%
GATTGTAAGGACTAAATTGT+TGG + chr3.4:92469096-92469115 None:intergenic 30.0%
GGTGGTAAAATTCCTTTATA+AGG + chr3.4:92469613-92469632 None:intergenic 30.0%
TTAAAACTTAAATGTGAGGG+AGG + chr3.4:92468406-92468425 None:intergenic 30.0%
TTTCCAGTTTCAAATATGAG+AGG + chr3.4:92468133-92468152 None:intergenic 30.0%
! ATAATGAATCCTTCTAGAGA+TGG + chr3.4:92470787-92470806 None:intergenic 30.0%
! ATCTCTAGAAGGATTCATTA+TGG - chr3.4:92470786-92470805 MS.gene80190:intron 30.0%
! ATTCCTCTCATATTTGAAAC+TGG - chr3.4:92468127-92468146 MS.gene80190:intron 30.0%
! CATTTCAATTTTCACTGCAT+TGG + chr3.4:92469224-92469243 None:intergenic 30.0%
! CCGTTTAAAACTTAAATGTG+AGG + chr3.4:92468410-92468429 None:intergenic 30.0%
! CCTTTATAAGGATTTTCTTC+AGG + chr3.4:92469601-92469620 None:intergenic 30.0%
! GGAAGACAACACAATTTTTA+AGG + chr3.4:92468630-92468649 None:intergenic 30.0%
! GTAGTGTGTAGAGTATTAAT+TGG + chr3.4:92468917-92468936 None:intergenic 30.0%
! TAGTGATCTTTCAATTTTGC+AGG - chr3.4:92470534-92470553 MS.gene80190:intron 30.0%
! TCCGATAAATTGAAGAGTTT+TGG + chr3.4:92468237-92468256 None:intergenic 30.0%
! TGTGTAGAGTATTAATTGGA+GGG + chr3.4:92468913-92468932 None:intergenic 30.0%
!! TCTAGGGCTATTTTATTTGA+TGG - chr3.4:92469697-92469716 MS.gene80190:intron 30.0%
!!! ACTCTTTTTTTCTTATCCTC+TGG - chr3.4:92468269-92468288 MS.gene80190:CDS 30.0%
!!! ATTGAAGAGTTTTGGATCAA+AGG + chr3.4:92468229-92468248 None:intergenic 30.0%
!!! TTCAAAATGAGTGTCGTTTT+AGG + chr3.4:92469047-92469066 None:intergenic 30.0%
AACAAGATCACTGTATACCT+TGG + chr3.4:92470874-92470893 None:intergenic 35.0%
AATAGGTAACCTCTTACATG+TGG + chr3.4:92468651-92468670 None:intergenic 35.0%
AATAGTATCTCGCTTGTACA+AGG - chr3.4:92471187-92471206 MS.gene80190:CDS 35.0%
AGAAGAGATAGTTGTGAAGA+GGG - chr3.4:92471085-92471104 MS.gene80190:CDS 35.0%
CATAAAATATGCAAGGTCTG+GGG - chr3.4:92468735-92468754 MS.gene80190:intron 35.0%
CCTAGAATATCAAATCACAC+AGG + chr3.4:92469683-92469702 None:intergenic 35.0%
CCTGTGTGATTTGATATTCT+AGG - chr3.4:92469680-92469699 MS.gene80190:intron 35.0%
GACAGTGCATAAAATATGCA+AGG - chr3.4:92468728-92468747 MS.gene80190:intron 35.0%
GATGAAGCAGCTTCAATTTA+TGG - chr3.4:92467839-92467858 MS.gene80190:CDS 35.0%
GCATAAAATATGCAAGGTCT+GGG - chr3.4:92468734-92468753 MS.gene80190:intron 35.0%
TAGAAGAGATAGTTGTGAAG+AGG - chr3.4:92471084-92471103 MS.gene80190:CDS 35.0%
TATACCTTGGCACGAATTAA+AGG + chr3.4:92470861-92470880 None:intergenic 35.0%
TGCATAAAATATGCAAGGTC+TGG - chr3.4:92468733-92468752 MS.gene80190:intron 35.0%
TTATGGAAAGTTTGTAGCTG+AGG - chr3.4:92470803-92470822 MS.gene80190:intron 35.0%
TTGTAAGGTTGAACAACATG+AGG - chr3.4:92468305-92468324 MS.gene80190:CDS 35.0%
TTGTAATGTGGCACAATTGA+GGG + chr3.4:92469634-92469653 None:intergenic 35.0%
! GAATGGAAGCATAATGTTGA+TGG + chr3.4:92468034-92468053 None:intergenic 35.0%
! GTGTGTAGAGTATTAATTGG+AGG + chr3.4:92468914-92468933 None:intergenic 35.0%
! TGTTATGCTGTTAAGTTGTC+CGG - chr3.4:92467869-92467888 MS.gene80190:CDS 35.0%
!! AAGCGACACTCATTTTGAAA+CGG + chr3.4:92468813-92468832 None:intergenic 35.0%
!! TCCTAGTCCTTTTAGGAATT+GGG - chr3.4:92470569-92470588 MS.gene80190:intron 35.0%
!!! AACGACACTCATTTTGAAAC+AGG - chr3.4:92469049-92469068 MS.gene80190:intron 35.0%
!!! GGTAAATGGTTTGTTTTTGC+AGG - chr3.4:92471022-92471041 MS.gene80190:CDS 35.0%
AATACACCCTAGAATCAGTG+AGG - chr3.4:92467928-92467947 MS.gene80190:CDS 40.0%
ACCTGTGAGAAGTTGTAATG+TGG + chr3.4:92469646-92469665 None:intergenic 40.0%
AGACACAGATCAACTGCAAA+TGG - chr3.4:92467894-92467913 MS.gene80190:CDS 40.0%
AGCCTTGATCATGATGTCAA+GGG - chr3.4:92471143-92471162 MS.gene80190:CDS 40.0%
AGGATGCATTAGTCAAACCA+AGG + chr3.4:92470740-92470759 None:intergenic 40.0%
ATACACCCTAGAATCAGTGA+GGG - chr3.4:92467929-92467948 MS.gene80190:CDS 40.0%
ATTAAAGGCTCATAGTCACG+AGG + chr3.4:92470846-92470865 None:intergenic 40.0%
CAGTACTCCCAATTCCTAAA+AGG + chr3.4:92470579-92470598 None:intergenic 40.0%
GAGGAATTAAGCAACTACCT+TGG + chr3.4:92468114-92468133 None:intergenic 40.0%
GGATGCATTAGTCAAACCAA+GGG + chr3.4:92470739-92470758 None:intergenic 40.0%
GTTGTAATGTGGCACAATTG+AGG + chr3.4:92469635-92469654 None:intergenic 40.0%
TACAAGCGAGATACTATTGC+TGG + chr3.4:92471185-92471204 None:intergenic 40.0%
TATCTCGCTTGTACAAGGAA+TGG - chr3.4:92471192-92471211 MS.gene80190:CDS 40.0%
TCCCAATTCCTAAAAGGACT+AGG + chr3.4:92470573-92470592 None:intergenic 40.0%
TGATGTCAAGGGACATTACA+AGG - chr3.4:92471154-92471173 MS.gene80190:CDS 40.0%
TGTGAAGAGGGTTGAAAAGA+AGG - chr3.4:92471097-92471116 MS.gene80190:CDS 40.0%
TGTTGTCTTCCACATGTAAG+AGG - chr3.4:92468639-92468658 MS.gene80190:CDS 40.0%
! GGTAGGTAAGAGTATGTTAG+TGG - chr3.4:92468540-92468559 MS.gene80190:intron 40.0%
! TGATCAGTGCTCTAAGTCTA+AGG - chr3.4:92469137-92469156 MS.gene80190:intron 40.0%
! TTTTCGGTCCTAGTCCTTTT+AGG - chr3.4:92470562-92470581 MS.gene80190:intron 40.0%
!! AGCATAATGTTGATGGTAGG+TGG + chr3.4:92468027-92468046 None:intergenic 40.0%
!! GCATAATGTTGATGGTAGGT+GGG + chr3.4:92468026-92468045 None:intergenic 40.0%
!! GGAAGCATAATGTTGATGGT+AGG + chr3.4:92468030-92468049 None:intergenic 40.0%
!! GGGCTTCACTTGTTTGTTTT+TGG - chr3.4:92468188-92468207 MS.gene80190:intron 40.0%
!! GTCCTAGTCCTTTTAGGAAT+TGG - chr3.4:92470568-92470587 MS.gene80190:intron 40.0%
!!! CTTCCGCTTTTTGTTGCTTT+CGG - chr3.4:92470481-92470500 MS.gene80190:intron 40.0%
!!! GACACTCATTTTGAAACAGG+AGG - chr3.4:92469052-92469071 MS.gene80190:intron 40.0%
!!! TTCCGCTTTTTGTTGCTTTC+GGG - chr3.4:92470482-92470501 MS.gene80190:intron 40.0%
AGGGCAAGAATCGTCTAAAG+TGG + chr3.4:92470720-92470739 None:intergenic 45.0%
CACTTCTTGACCAAACACCA+TGG + chr3.4:92471123-92471142 None:intergenic 45.0%
CTTTAGACGATTCTTGCCCT+TGG - chr3.4:92470720-92470739 MS.gene80190:intron 45.0%
GAAAAGAAGGCCATGGTGTT+TGG - chr3.4:92471110-92471129 MS.gene80190:CDS 45.0%
GAGCCTTGATCATGATGTCA+AGG - chr3.4:92471142-92471161 MS.gene80190:CDS 45.0%
GCAGCTTCAATTTATGGCTG+TGG - chr3.4:92467845-92467864 MS.gene80190:CDS 45.0%
GCCACATTACAACTTCTCAC+AGG - chr3.4:92469642-92469661 MS.gene80190:intron 45.0%
GGTAGGTGGGAGAATTAAGA+GGG + chr3.4:92468013-92468032 None:intergenic 45.0%
GTCCCTTGACATCATGATCA+AGG + chr3.4:92471148-92471167 None:intergenic 45.0%
TAATGTGGCACAATTGAGGG+TGG + chr3.4:92469631-92469650 None:intergenic 45.0%
TACTGAAGCCATTCAAGCCA+AGG - chr3.4:92468094-92468113 MS.gene80190:intron 45.0%
TCCCCGAAAGCAACAAAAAG+CGG + chr3.4:92470487-92470506 None:intergenic 45.0%
TGAGCCTTTAATTCGTGCCA+AGG - chr3.4:92470854-92470873 MS.gene80190:intron 45.0%
TGGTAGGTGGGAGAATTAAG+AGG + chr3.4:92468014-92468033 None:intergenic 45.0%
! AGCAACTACCTTGGCTTGAA+TGG + chr3.4:92468105-92468124 None:intergenic 45.0%
! GGGCAAGAATCGTCTAAAGT+GGG + chr3.4:92470719-92470738 None:intergenic 45.0%
!! AAGATGCCCTCACTGATTCT+AGG + chr3.4:92467938-92467957 None:intergenic 45.0%
!! AGATGCCCTCACTGATTCTA+GGG + chr3.4:92467937-92467956 None:intergenic 45.0%
!! GCTTCCATTCCTCACTTTTC+CGG - chr3.4:92468044-92468063 MS.gene80190:CDS 45.0%
!! GGGAGTACTGTATAATCCCT+TGG - chr3.4:92470589-92470608 MS.gene80190:intron 45.0%
!! TTTGTTGCTTTCGGGGATGA+TGG - chr3.4:92470490-92470509 MS.gene80190:intron 45.0%
!!! TCCGCTTTTTGTTGCTTTCG+GGG - chr3.4:92470483-92470502 MS.gene80190:intron 45.0%
!!! AATTTTATTCTTTTTTTTAA+TGG + chr3.4:92470265-92470284 None:intergenic 5.0%
!!! TTGATTTTTTTTTTTTTTTA+CGG + chr3.4:92469969-92469988 None:intergenic 5.0%
AACGACATCGAGCAACGAAC+CGG + chr3.4:92468066-92468085 None:intergenic 50.0%
AGCAACGAACCGGAAAAGTG+AGG + chr3.4:92468056-92468075 None:intergenic 50.0%
ATTCGCAGGCCATCTCTAGA+AGG - chr3.4:92470775-92470794 MS.gene80190:intron 50.0%
CGAACCGGAAAAGTGAGGAA+TGG + chr3.4:92468051-92468070 None:intergenic 50.0%
GAGGGTTGAAAAGAAGGCCA+TGG - chr3.4:92471103-92471122 MS.gene80190:CDS 50.0%
GCAGTTGATCTGTGTCTCTC+CGG + chr3.4:92467891-92467910 None:intergenic 50.0%
!! CAAAAAGCGGAAGCATCTCG+TGG + chr3.4:92470474-92470493 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 92467837 92471271 92467837 ID=MS.gene80190
chr3.4 mRNA 92467837 92471271 92467837 ID=MS.gene80190.t1;Parent=MS.gene80190
chr3.4 exon 92470993 92471271 92470993 ID=MS.gene80190.t1.exon1;Parent=MS.gene80190.t1
chr3.4 CDS 92470993 92471271 92470993 ID=cds.MS.gene80190.t1;Parent=MS.gene80190.t1
chr3.4 exon 92469445 92469528 92469445 ID=MS.gene80190.t1.exon2;Parent=MS.gene80190.t1
chr3.4 CDS 92469445 92469528 92469445 ID=cds.MS.gene80190.t1;Parent=MS.gene80190.t1
chr3.4 exon 92468553 92468696 92468553 ID=MS.gene80190.t1.exon3;Parent=MS.gene80190.t1
chr3.4 CDS 92468553 92468696 92468553 ID=cds.MS.gene80190.t1;Parent=MS.gene80190.t1
chr3.4 exon 92468233 92468325 92468233 ID=MS.gene80190.t1.exon4;Parent=MS.gene80190.t1
chr3.4 CDS 92468233 92468325 92468233 ID=cds.MS.gene80190.t1;Parent=MS.gene80190.t1
chr3.4 exon 92467837 92468064 92467837 ID=MS.gene80190.t1.exon5;Parent=MS.gene80190.t1
chr3.4 CDS 92467837 92468064 92467837 ID=cds.MS.gene80190.t1;Parent=MS.gene80190.t1
Gene Sequence

>MS.gene80190

ATGATGAAGCAGCTTCAATTTATGGCTGTGGTTGTTATGCTGTTAAGTTGTCCGGAGAGACACAGATCAACTGCAAATGGTTCAGAGAATGAATACACCCTAGAATCAGTGAGGGCATCTTTAATTAGACAAGAAGATACAATCATATTTAGTGTGATTGAGAGAGCAAGATTCCCTCTTAATTCTCCCACCTACCATCAACATTATGCTTCCATTCCTCACTTTTCCGGTTCGTTGCTCGATGTCGTTCTTGACTATACTGAAGCCATTCAAGCCAAGGTAGTTGCTTAATTCCTCTCATATTTGAAACTGGAAATCTTTTATGTTAATGATTTTGTATTAGAATTTATTGGGCTTCACTTGTTTGTTTTTGGTGATTGTTCTAATGACCTTTGATCCAAAACTCTTCAATTTATCGGACCGCATCTTCCTACTCTTTTTTTCTTATCCTCTGGTTTTTTTTTTTTTTTGTAAGGTTGAACAACATGAGGAACTTATATAGAGCAATTATCTGCGTTCTTTTTTTACCTATGCATCTTGTTTTATTTATGTGTATGTGTTTTCTTCCTCCCTCACATTTAAGTTTTAAACGGTTAACAAAATAATAATAAACAATATTGATTTTAATCGTAAAATATGTATTATTTGTTAAAACACTTTTATTAATTATAGTTAGTTAAGTAGTTATAGATGATTAAAATACGGTAGGTAAGAGTATGTTAGTGGAAAATAATAAAGGATCTAATGATATTTTAAAAGGGACAAATAAAATGTGTATGCGTGTTTAGATCCTTAAAAATTGTGTTGTCTTCCACATGTAAGAGGTTACCTATTTATTTTTGGCTTTATTTTTTTTCTACATAATACATATGCAACAATAAAGAATGTATGGACAGTGCATAAAATATGCAAGGTCTGGGGTTCAAACCCCGACCATCAAAAAAAATAAAGAATGTGTCTTATTATACTCTCTCCGTTTCAAAATGAGTGTCGCTTTAACCAAAACAATTTGTTTCAAAATGAATGTCACTTTCAGTTTCCAATGCAATAATAACTTTTTCTTTTCAATTATACCCTCCAATTAATACTCTACACACTACTTCCAAATTATTACTTTTAAAACTAACCAATAGTTAATAAGGATAATTTTGTAAAATAACTATTCTCTTTCTTTTAATCATTGCATTTCTTAATATGTGTGTAAACACCTAAAACGACACTCATTTTGAAACAGGAGGAGTATTAGATAAGTTGAACCAACAATTTAGTCCTTACAATCGAATTAATGAAAGAGAAATGGTGATCAGTGCTCTAAGTCTAAGGACATTTTTTAAGAATCTTAAATACAATAGGAAATTTTTGTGTGAATTACTAAACTTTGCGTCCAATGCAGTGAAAATTGAAATGTTTGACAATAAATTTTTTAAATAAGATTATTTAAAGAGTACGTTTAAAACACTTGTGAGCATGACCACGAAATGAAAATTTAATGAAATTAACAAAATTTCAAAATTAAAATGTCCTTGATTTTCATACAATTTTGAAAAACAAAATTGAGCAAAGATTTATTAGAAAATATTTTCGATTTTAAAAACCGTCACATGATTACAACTTAATTTTTTCGTACAACAACTTACTTTAGTGACACCTAAATTTTCTGTATTAAATAAATTGGTGGTTTACTTTATTATATCTATGTCAATGAGTTATTTATGTTGATTGCTTTGAAATATTATTCGTTAGTTAGGAAGATACATCAACCCTGAAGAAAATCCTTATAAAGGAATTTTACCACCCTCAATTGTGCCACATTACAACTTCTCACAGGTAATTCACTAAAACTCCTGTGTGATTTGATATTCTAGGGCTATTTTATTTGATGGTAGTAAAAATAATGGTCATGTTGACTAGTATCTTCTGAATATTCATTGATGAAACAGAAAAAAAATACATTTTGTATTGATTTTTTTTTTTCACTTTTAAGATATTGACTACATGTATAAGTTTTAAGACAATTTTGTTATATTTGGTTTCTTAACAAGTGCTCTAATGCTTCTATTTTTCTAAAAATAATCTTTGGAAATGCGGAGATTAGAAATTTGAATTCTTTTTCTTTTGTTGTGTGTAAACCGTAAAAAAAAAAAAAAATCAAAAAGAAGAAGAAAAGGAAAATGAAGAAGATTAGAAATTTGAATTCCTATACATATTACAAAGATAGAAAATTTAGTCCCCAGTAAAACATATTTTTGAGAATAAAATAATTTTCATTTCTAATGCTTTTATATCAAAGGAAGATATTGGAGAAAAGGGATCTTCATGAAGATGAAAATAAAATTATGCAATTGCCTCGATTACAGATAATTCTAAGGAGTTTCACAAGTGTATGCCAAACCCTTTTCTAATCAATTCAAGAGAATAAATTTAGCCATTAAAAAAAAGAATAAAATTACAAATGTTTTTTTTTAATAAATAAAATTACAAATGTTATGTTCCATAAAATTTATTCGTGGAAATAAATATCTCAATTCTCAACCATGAAAAGAAAATAAGGATCTCTTTTGAATTTTGTCTTTAGTTTTTGCATGCTGCAGCCGCTTCGATTAACATAAACAAGAACATAAGGAAAGTGTATTTCCACGAGATGCTTCCGCTTTTTGTTGCTTTCGGGGATGATGGCAACTATGCACAAACTGCAGCTAGTGATCTTTCAATTTTGCAGGTAAAATTTTCGGTCCTAGTCCTTTTAGGAATTGGGAGTACTGTATAATCCCTTGGTTCTTATTAACAAGATAAAGATGGATATCGTAGGTACATCATAACAAATTATAAGAATATGTTATAATAATAAGCCTTGATTTTCTCATTTTTACATTTAATTACCCACTTTAGACGATTCTTGCCCTTGGTTTGACTAATGCATCCTTATTCTATAAAATATATTCGCAGGCCATCTCTAGAAGGATTCATTATGGAAAGTTTGTAGCTGAGGCGAAGTTCAGAGAATCTCCTCGTGACTATGAGCCTTTAATTCGTGCCAAGGTATACAGTGATCTTGTTCATAAGAAGAAAATAAAAACTAATAGAAATATTTTTGCAATATTTCTAGACATTGTTCTACTATCGTTTTTTAAATGCAAAACTTGGTTTTTTATGTTGTTACTACGTTTTGGTGTAAAAATTGAAACGGTAAATGGTTTGTTTTTGCAGGACAGAAAAGCACTGATGAAATTGTTGACATCGAAAAATGTAGAAGAGATAGTTGTGAAGAGGGTTGAAAAGAAGGCCATGGTGTTTGGTCAAGAAGTGAGCCTTGATCATGATGTCAAGGGACATTACAAGGTTGATCCAGCAATAGTATCTCGCTTGTACAAGGAATGGATAATACCCATGACTAAAAATGTTGAACTTGAGTACTTACTGCGTCGTCTCGATTGA
Protein sequence

>MS.gene80190.t1

MMKQLQFMAVVVMLLSCPERHRSTANGSENEYTLESVRASLIRQEDTIIFSVIERARFPLNSPTYHQHYASIPHFSGSLLDVVLDYTEAIQAKLGRYINPEENPYKGILPPSIVPHYNFSQFLHAAAASININKNIRKVYFHEMLPLFVAFGDDGNYAQTAASDLSILQAISRRIHYGKFVAEAKFRESPRDYEPLIRAKDRKALMKLLTSKNVEEIVVKRVEKKAMVFGQEVSLDHDVKGHYKVDPAIVSRLYKEWIIPMTKNVELEYLLRRLD