Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80271.t1 | XP_003613228.1 | 98.6 | 278 | 4 | 0 | 1 | 278 | 1 | 278 | 3.50E-156 | 560.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80271.t1 | P27141 | 71.4 | 262 | 74 | 1 | 18 | 278 | 60 | 321 | 5.1e-108 | 392.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80271.t1 | G7K1D4 | 98.6 | 278 | 4 | 0 | 1 | 278 | 1 | 278 | 2.5e-156 | 560.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene061224 | MS.gene80271 | 0.833286 | 5.82E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80271.t1 | MTR_5g034250 | 98.561 | 278 | 4 | 0 | 1 | 278 | 1 | 278 | 0.0 | 570 |
| MS.gene80271.t1 | MTR_5g034250 | 98.438 | 256 | 4 | 0 | 23 | 278 | 1 | 256 | 0.0 | 525 |
| MS.gene80271.t1 | MTR_8g064630 | 81.250 | 256 | 47 | 1 | 23 | 278 | 1 | 255 | 1.54e-157 | 439 |
| MS.gene80271.t1 | MTR_6g006990 | 63.158 | 266 | 92 | 2 | 18 | 278 | 67 | 331 | 3.78e-122 | 352 |
| MS.gene80271.t1 | MTR_6g006990 | 63.707 | 259 | 89 | 1 | 25 | 278 | 2 | 260 | 5.53e-122 | 349 |
| MS.gene80271.t1 | MTR_8g064630 | 85.714 | 175 | 24 | 1 | 103 | 277 | 6 | 179 | 3.80e-112 | 321 |
| MS.gene80271.t1 | MTR_3g077910 | 54.475 | 257 | 116 | 1 | 23 | 278 | 33 | 289 | 5.12e-94 | 279 |
| MS.gene80271.t1 | MTR_3g077940 | 52.529 | 257 | 121 | 1 | 23 | 278 | 1 | 257 | 1.10e-92 | 275 |
| MS.gene80271.t1 | MTR_6g006990 | 61.321 | 212 | 76 | 2 | 18 | 224 | 67 | 277 | 3.87e-91 | 271 |
| MS.gene80271.t1 | MTR_3g077940 | 57.592 | 191 | 81 | 0 | 88 | 278 | 28 | 218 | 2.64e-75 | 229 |
| MS.gene80271.t1 | MTR_2g009500 | 51.777 | 197 | 92 | 2 | 74 | 270 | 89 | 282 | 3.11e-70 | 219 |
| MS.gene80271.t1 | MTR_5g066060 | 50.254 | 197 | 96 | 2 | 74 | 270 | 94 | 288 | 3.83e-63 | 201 |
| MS.gene80271.t1 | MTR_5g066060 | 51.299 | 154 | 73 | 2 | 74 | 227 | 94 | 245 | 9.33e-46 | 154 |
| MS.gene80271.t1 | MTR_3g077930 | 48.000 | 175 | 68 | 3 | 104 | 278 | 40 | 191 | 5.26e-42 | 143 |
| MS.gene80271.t1 | MTR_5g066060 | 52.809 | 89 | 41 | 1 | 74 | 162 | 94 | 181 | 2.16e-24 | 97.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80271.t1 | AT5G14740 | 69.884 | 259 | 75 | 1 | 23 | 278 | 1 | 259 | 2.01e-135 | 383 |
| MS.gene80271.t1 | AT5G14740 | 69.884 | 259 | 75 | 1 | 23 | 278 | 1 | 259 | 2.01e-135 | 383 |
| MS.gene80271.t1 | AT5G14740 | 69.884 | 259 | 75 | 1 | 23 | 278 | 1 | 259 | 2.01e-135 | 383 |
| MS.gene80271.t1 | AT5G14740 | 69.884 | 259 | 75 | 1 | 23 | 278 | 1 | 259 | 2.01e-135 | 383 |
| MS.gene80271.t1 | AT5G14740 | 69.884 | 259 | 75 | 1 | 23 | 278 | 1 | 259 | 2.01e-135 | 383 |
| MS.gene80271.t1 | AT5G14740 | 66.909 | 275 | 87 | 2 | 8 | 278 | 57 | 331 | 2.88e-134 | 383 |
| MS.gene80271.t1 | AT1G70410 | 64.158 | 279 | 99 | 1 | 1 | 278 | 1 | 279 | 5.92e-132 | 375 |
| MS.gene80271.t1 | AT5G14740 | 66.545 | 275 | 87 | 3 | 8 | 278 | 57 | 330 | 1.05e-131 | 376 |
| MS.gene80271.t1 | AT5G14740 | 65.818 | 275 | 89 | 3 | 8 | 278 | 57 | 330 | 1.52e-130 | 373 |
| MS.gene80271.t1 | AT3G01500 | 66.023 | 259 | 85 | 1 | 23 | 278 | 1 | 259 | 1.87e-126 | 360 |
| MS.gene80271.t1 | AT3G01500 | 66.023 | 259 | 85 | 1 | 23 | 278 | 1 | 259 | 3.30e-126 | 360 |
| MS.gene80271.t1 | AT3G01500 | 65.900 | 261 | 86 | 1 | 21 | 278 | 76 | 336 | 5.00e-126 | 362 |
| MS.gene80271.t1 | AT3G01500 | 65.900 | 261 | 86 | 1 | 21 | 278 | 76 | 336 | 1.18e-125 | 362 |
| MS.gene80271.t1 | AT1G70410 | 65.759 | 257 | 87 | 1 | 23 | 278 | 1 | 257 | 8.96e-125 | 356 |
| MS.gene80271.t1 | AT1G70410 | 65.759 | 257 | 87 | 1 | 23 | 278 | 1 | 257 | 8.96e-125 | 356 |
| MS.gene80271.t1 | AT1G23730 | 62.646 | 257 | 95 | 1 | 23 | 278 | 1 | 257 | 1.31e-121 | 348 |
| MS.gene80271.t1 | AT1G23730 | 62.646 | 257 | 95 | 1 | 23 | 278 | 1 | 257 | 1.31e-121 | 348 |
| MS.gene80271.t1 | AT5G14740 | 68.780 | 205 | 61 | 1 | 23 | 224 | 73 | 277 | 1.22e-101 | 299 |
| MS.gene80271.t1 | AT4G33580 | 47.442 | 215 | 106 | 4 | 61 | 270 | 67 | 279 | 1.61e-63 | 202 |
| MS.gene80271.t1 | AT4G33580 | 47.442 | 215 | 106 | 4 | 61 | 270 | 67 | 279 | 1.61e-63 | 202 |
| MS.gene80271.t1 | AT4G33580 | 47.222 | 216 | 106 | 5 | 61 | 270 | 67 | 280 | 6.48e-63 | 200 |
| MS.gene80271.t1 | AT1G58180 | 38.956 | 249 | 149 | 2 | 21 | 269 | 28 | 273 | 9.73e-61 | 194 |
| MS.gene80271.t1 | AT1G58180 | 38.956 | 249 | 149 | 2 | 21 | 269 | 28 | 273 | 9.73e-61 | 194 |
| MS.gene80271.t1 | AT1G58180 | 41.441 | 222 | 129 | 1 | 48 | 269 | 2 | 222 | 9.13e-60 | 190 |
| MS.gene80271.t1 | AT1G58180 | 41.441 | 222 | 129 | 1 | 48 | 269 | 2 | 222 | 9.13e-60 | 190 |
| MS.gene80271.t1 | AT1G58180 | 41.441 | 222 | 129 | 1 | 48 | 269 | 2 | 222 | 9.13e-60 | 190 |
| MS.gene80271.t1 | AT1G58180 | 40.265 | 226 | 132 | 2 | 21 | 246 | 28 | 250 | 5.82e-56 | 181 |
| MS.gene80271.t1 | AT1G58180 | 40.196 | 204 | 119 | 2 | 21 | 224 | 28 | 228 | 7.07e-48 | 160 |
Find 82 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTACCCTCTGTATCCATTTA+AGG | 0.143367 | 5.4:+24225550 | MS.gene80271:CDS |
| ACCTTGCTGGATTGCAAATT+TGG | 0.156469 | 5.4:-24225165 | None:intergenic |
| AGTATGCCCTTCTCACATTT+TGG | 0.186284 | 5.4:+24222392 | MS.gene80271:CDS |
| ATATGCGGTCTTACATTTAA+AGG | 0.232845 | 5.4:+24223113 | MS.gene80271:CDS |
| CTTGCGGTGGTCCCATCATC+TGG | 0.259227 | 5.4:-24224239 | None:intergenic |
| CGCTCCTGCTCCCGAATACT+TGG | 0.298559 | 5.4:-24223082 | None:intergenic |
| GACCTTAGGGCTCTGGCCTT+TGG | 0.303340 | 5.4:-24222020 | None:intergenic |
| AATTTAACCTTGGACCTTGC+TGG | 0.313505 | 5.4:-24225178 | None:intergenic |
| CAGCTGCTGTGGAGGCATAA+AGG | 0.320529 | 5.4:+24224202 | MS.gene80271:CDS |
| AGCTGCTGTGGAGGCATAAA+GGG | 0.327110 | 5.4:+24224203 | MS.gene80271:CDS |
| AAATGGATACAGAGGGTAAA+AGG | 0.342781 | 5.4:-24225546 | None:intergenic |
| GGTGTTGGAGAGACCTTAAA+TGG | 0.356865 | 5.4:-24225563 | None:intergenic |
| GGAGCTGACGGCGGAGTTAG+AGG | 0.369439 | 5.4:+24220949 | MS.gene80271:CDS |
| CAGTGATTTCATTGAGCAAT+GGG | 0.388371 | 5.4:+24225141 | MS.gene80271:intron |
| CCAACCGGGTGAAGCCTTTG+TGG | 0.395118 | 5.4:+24222419 | MS.gene80271:CDS |
| GTGGAGAATATTGTAGTCAT+TGG | 0.437839 | 5.4:+24224176 | MS.gene80271:CDS |
| ATCCTCTCATCCGGGTTAAA+CGG | 0.445021 | 5.4:-24220986 | None:intergenic |
| GAAAAGTAAACCTTGTCATA+TGG | 0.446345 | 5.4:-24222468 | None:intergenic |
| GAACCATGTTGGCGATGTTA+CGG | 0.461219 | 5.4:-24222443 | None:intergenic |
| GTTAAACGGCTTTGAACCGT+TGG | 0.469755 | 5.4:-24220972 | None:intergenic |
| CCGGATCGGATCCTCTCATC+CGG | 0.470107 | 5.4:-24220995 | None:intergenic |
| CTCATGTCTATCCCAGATGA+TGG | 0.470494 | 5.4:+24224227 | MS.gene80271:CDS |
| GAAGCTGTGAATGTATCTCT+TGG | 0.472140 | 5.4:+24225406 | MS.gene80271:CDS |
| GCAAGTTCACCGTACAGCTC+AGG | 0.475420 | 5.4:-24221998 | None:intergenic |
| AGTCTTGAAACTAACAAAAC+CGG | 0.477828 | 5.4:-24221014 | None:intergenic |
| ACAACACCATCTCTCACAAA+TGG | 0.478188 | 5.4:-24225445 | None:intergenic |
| CGGTTCAAAGCCGTTTAACC+CGG | 0.492215 | 5.4:+24220976 | MS.gene80271:CDS |
| ATGTAAGACCGCATATTCAA+TGG | 0.508542 | 5.4:-24223106 | None:intergenic |
| TTCAGGGAGAAAGCTGAGCT+TGG | 0.509469 | 5.4:+24220902 | MS.gene80271:intron |
| AATAGCTGACCTTAGGGCTC+TGG | 0.511436 | 5.4:-24222027 | None:intergenic |
| ACTTGTTTCTAATTTAACCT+TGG | 0.514008 | 5.4:-24225188 | None:intergenic |
| AAGAAAACCCTTGCTTTGAA+AGG | 0.517445 | 5.4:+24225469 | MS.gene80271:CDS |
| AAGCTGTGAATGTATCTCTT+GGG | 0.518223 | 5.4:+24225407 | MS.gene80271:CDS |
| CAGACCAAGTATTCGGGAGC+AGG | 0.520906 | 5.4:+24223078 | MS.gene80271:intron |
| GACCAAAGAGAGAAATGGCT+GGG | 0.523370 | 5.4:+24215246 | None:intergenic |
| CGTCAGCTCCTTGATCTTCA+CGG | 0.527964 | 5.4:-24220936 | None:intergenic |
| ACAGTGATTTCATTGAGCAA+TGG | 0.531537 | 5.4:+24225140 | MS.gene80271:intron |
| ATGTGCTCCTTTCAAAGCAA+GGG | 0.535168 | 5.4:-24225476 | None:intergenic |
| CATCGCCGCCGTGAAGATCA+AGG | 0.541374 | 5.4:+24220928 | MS.gene80271:CDS |
| TTGCGGTGGTCCCATCATCT+GGG | 0.549867 | 5.4:-24224238 | None:intergenic |
| CAAGTATTCGGGAGCAGGAG+CGG | 0.551194 | 5.4:+24223083 | MS.gene80271:CDS |
| GCAGTGCACAAACTGTGAAA+AGG | 0.551365 | 5.4:+24225228 | MS.gene80271:CDS |
| ACTTATCCATTTGTGAGAGA+TGG | 0.556933 | 5.4:+24225439 | MS.gene80271:CDS |
| AATGTGCTCCTTTCAAAGCA+AGG | 0.563009 | 5.4:-24225477 | None:intergenic |
| AATCAGAAGGAAGACATGGC+TGG | 0.564330 | 5.4:+24220656 | MS.gene80271:intron |
| TTGCACAAGCAAGATTGTCT+CGG | 0.566591 | 5.4:+24215295 | MS.gene80271:CDS |
| CCGGATGAGAGGATCCGATC+CGG | 0.568309 | 5.4:+24220995 | MS.gene80271:CDS |
| AAGTCTGGTCAGAGTTGCGA+TGG | 0.569242 | 5.4:-24220696 | None:intergenic |
| TGGAAATCCAAAATGTGAGA+AGG | 0.571528 | 5.4:-24222399 | None:intergenic |
| GTCATTGGACACAGCTGCTG+TGG | 0.574698 | 5.4:+24224191 | MS.gene80271:CDS |
| TCCAAATTTGCAATCCAGCA+AGG | 0.577092 | 5.4:+24225164 | MS.gene80271:CDS |
| CCACAAAGGCTTCACCCGGT+TGG | 0.578743 | 5.4:-24222419 | None:intergenic |
| GGACCAAAGAGAGAAATGGC+TGG | 0.579000 | 5.4:+24215245 | None:intergenic |
| ATTGGACACAGCTGCTGTGG+AGG | 0.579655 | 5.4:+24224194 | MS.gene80271:CDS |
| GGAAATCCAAAATGTGAGAA+GGG | 0.582778 | 5.4:-24222398 | None:intergenic |
| AAATAAAACTTGCCTTGCGG+TGG | 0.586782 | 5.4:-24224252 | None:intergenic |
| CGGACCACAAAGGCTTCACC+CGG | 0.592537 | 5.4:-24222423 | None:intergenic |
| TGCCAAAGGCCAGAGCCCTA+AGG | 0.593445 | 5.4:+24222018 | MS.gene80271:CDS |
| TCATGTCTATCCCAGATGAT+GGG | 0.593762 | 5.4:+24224228 | MS.gene80271:CDS |
| TTGCAATCCAGCAAGGTCCA+AGG | 0.594272 | 5.4:+24225171 | MS.gene80271:CDS |
| AGGAAGAATCCTGAGCTGTA+CGG | 0.595793 | 5.4:+24221989 | MS.gene80271:intron |
| AAGTAAACCTTGTCATATGG+TGG | 0.601200 | 5.4:-24222465 | None:intergenic |
| AGCCGTTTAACCCGGATGAG+AGG | 0.610912 | 5.4:+24220984 | MS.gene80271:CDS |
| CATGGTTCCACCATATGACA+AGG | 0.613750 | 5.4:+24222458 | MS.gene80271:CDS |
| CATGGCTGGAGAAACATTCG+AGG | 0.615024 | 5.4:+24220670 | MS.gene80271:CDS |
| CGGATCGGATCCTCTCATCC+GGG | 0.617375 | 5.4:-24220994 | None:intergenic |
| GTCATATGGTGGAACCATGT+TGG | 0.618619 | 5.4:-24222454 | None:intergenic |
| GGTCCGTAACATCGCCAACA+TGG | 0.627271 | 5.4:+24222440 | MS.gene80271:CDS |
| CAGATGATGGGACCACCGCA+AGG | 0.627584 | 5.4:+24224240 | MS.gene80271:CDS |
| TGAAACTAACAAAACCGGAT+CGG | 0.630831 | 5.4:-24221009 | None:intergenic |
| ATGAAATCAGAAGGAAGACA+TGG | 0.641190 | 5.4:+24220652 | MS.gene80271:intron |
| ACCAAAGAGAGAAATGGCTG+GGG | 0.642666 | 5.4:+24215247 | None:intergenic |
| CTGTACGGTGAACTTGCCAA+AGG | 0.647222 | 5.4:+24222004 | MS.gene80271:CDS |
| GAGACCTTAAATGGATACAG+AGG | 0.653917 | 5.4:-24225554 | None:intergenic |
| AGGAGCGGCCATTGAATATG+CGG | 0.655268 | 5.4:+24223098 | MS.gene80271:CDS |
| CGTGAAGATCAAGGAGCTGA+CGG | 0.657927 | 5.4:+24220937 | MS.gene80271:CDS |
| ACGGCGGAGTTAGAGGCCAA+CGG | 0.658529 | 5.4:+24220956 | MS.gene80271:CDS |
| GCTGCTGTGGAGGCATAAAG+GGG | 0.658582 | 5.4:+24224204 | MS.gene80271:CDS |
| GGCGATGTTACGGACCACAA+AGG | 0.665172 | 5.4:-24222433 | None:intergenic |
| AGACCTTAAATGGATACAGA+GGG | 0.665728 | 5.4:-24225553 | None:intergenic |
| GAAGATCAAGGAGCTGACGG+CGG | 0.707015 | 5.4:+24220940 | MS.gene80271:CDS |
| CAGCTCCTTGATCTTCACGG+CGG | 0.827966 | 5.4:-24220933 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 24215260 | 24225570 | 24215260 | ID=MS.gene80271 |
| chr5.4 | mRNA | 24215260 | 24225570 | 24215260 | ID=MS.gene80271.t1;Parent=MS.gene80271 |
| chr5.4 | exon | 24215260 | 24215316 | 24215260 | ID=MS.gene80271.t1.exon1;Parent=MS.gene80271.t1 |
| chr5.4 | CDS | 24215260 | 24215316 | 24215260 | ID=cds.MS.gene80271.t1;Parent=MS.gene80271.t1 |
| chr5.4 | exon | 24220662 | 24220723 | 24220662 | ID=MS.gene80271.t1.exon2;Parent=MS.gene80271.t1 |
| chr5.4 | CDS | 24220662 | 24220723 | 24220662 | ID=cds.MS.gene80271.t1;Parent=MS.gene80271.t1 |
| chr5.4 | exon | 24220907 | 24221047 | 24220907 | ID=MS.gene80271.t1.exon3;Parent=MS.gene80271.t1 |
| chr5.4 | CDS | 24220907 | 24221047 | 24220907 | ID=cds.MS.gene80271.t1;Parent=MS.gene80271.t1 |
| chr5.4 | exon | 24221991 | 24222039 | 24221991 | ID=MS.gene80271.t1.exon4;Parent=MS.gene80271.t1 |
| chr5.4 | CDS | 24221991 | 24222039 | 24221991 | ID=cds.MS.gene80271.t1;Parent=MS.gene80271.t1 |
| chr5.4 | exon | 24222363 | 24222479 | 24222363 | ID=MS.gene80271.t1.exon5;Parent=MS.gene80271.t1 |
| chr5.4 | CDS | 24222363 | 24222479 | 24222363 | ID=cds.MS.gene80271.t1;Parent=MS.gene80271.t1 |
| chr5.4 | exon | 24223081 | 24223134 | 24223081 | ID=MS.gene80271.t1.exon6;Parent=MS.gene80271.t1 |
| chr5.4 | CDS | 24223081 | 24223134 | 24223081 | ID=cds.MS.gene80271.t1;Parent=MS.gene80271.t1 |
| chr5.4 | exon | 24224176 | 24224261 | 24224176 | ID=MS.gene80271.t1.exon7;Parent=MS.gene80271.t1 |
| chr5.4 | CDS | 24224176 | 24224261 | 24224176 | ID=cds.MS.gene80271.t1;Parent=MS.gene80271.t1 |
| chr5.4 | exon | 24225144 | 24225249 | 24225144 | ID=MS.gene80271.t1.exon8;Parent=MS.gene80271.t1 |
| chr5.4 | CDS | 24225144 | 24225249 | 24225144 | ID=cds.MS.gene80271.t1;Parent=MS.gene80271.t1 |
| chr5.4 | exon | 24225406 | 24225570 | 24225406 | ID=MS.gene80271.t1.exon9;Parent=MS.gene80271.t1 |
| chr5.4 | CDS | 24225406 | 24225570 | 24225406 | ID=cds.MS.gene80271.t1;Parent=MS.gene80271.t1 |
| Gene Sequence |
| Protein sequence |
