Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80426.t1 | RHN63488.1 | 88.1 | 67 | 8 | 0 | 1 | 67 | 150 | 216 | 4.90E-24 | 119.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80426.t1 | A0A072UQ35 | 88.1 | 67 | 8 | 0 | 1 | 67 | 150 | 216 | 3.5e-24 | 119.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05329 | MS.gene80426 | 0.809139 | 2.19E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 10 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTAAATACTTGAATTTCT+TGG | 0.237378 | 6.3:-29784073 | MS.gene80426:intron |
| CTTGCAATATTTGATCTTAG+AGG | 0.267024 | 6.3:-29784117 | MS.gene80426:CDS |
| ATTGTCTCAAGTATTATTTG+TGG | 0.480852 | 6.3:-29783896 | MS.gene80426:CDS |
| CATTGAGTAAGCTTCCCACT+TGG | 0.513570 | 6.3:-29784152 | MS.gene80426:CDS |
| AATACTTGAGACAATCGATT+GGG | 0.532364 | 6.3:+29783904 | None:intergenic |
| ATTGAGTAAGCTTCCCACTT+GGG | 0.558820 | 6.3:-29784151 | MS.gene80426:CDS |
| TAATACTTGAGACAATCGAT+TGG | 0.563337 | 6.3:+29783903 | None:intergenic |
| AGTATTTGCTCTTCCCAAGT+GGG | 0.594021 | 6.3:+29784138 | None:intergenic |
| AAGTATTTGCTCTTCCCAAG+TGG | 0.630764 | 6.3:+29784137 | None:intergenic |
| AGGTCCCCGCAGATGATCAG+AGG | 0.632925 | 6.3:-29784200 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTTAAATACTTGAATTTCT+TGG | - | chr6.3:29784000-29784019 | MS.gene80426:intron | 20.0% |
| !! | TTTGTTCAAGATTAGAAAAT+AGG | + | chr6.3:29784087-29784106 | None:intergenic | 20.0% |
| !!! | ACTTATCGACTAAAAATATT+TGG | + | chr6.3:29784050-29784069 | None:intergenic | 20.0% |
| !!! | CAAATACATTTCTTTACTTT+TGG | - | chr6.3:29784119-29784138 | MS.gene80426:CDS | 20.0% |
| ! | ATTGTCTCAAGTATTATTTG+TGG | - | chr6.3:29784177-29784196 | MS.gene80426:CDS | 25.0% |
| ! | TTTCTAATCTTGAACAAAAG+TGG | - | chr6.3:29784089-29784108 | MS.gene80426:CDS | 25.0% |
| !!! | TATTTAAGATCAGCATTTTC+TGG | + | chr6.3:29783989-29784008 | None:intergenic | 25.0% |
| AATACTTGAGACAATCGATT+GGG | + | chr6.3:29784172-29784191 | None:intergenic | 30.0% | |
| CTTGCAATATTTGATCTTAG+AGG | - | chr6.3:29783956-29783975 | MS.gene80426:intron | 30.0% | |
| TAATACTTGAGACAATCGAT+TGG | + | chr6.3:29784173-29784192 | None:intergenic | 30.0% | |
| ! | GTGTGTTTTCTTAATTGAGA+AGG | - | chr6.3:29783898-29783917 | MS.gene80426:CDS | 30.0% |
| AAGTATTTGCTCTTCCCAAG+TGG | + | chr6.3:29783939-29783958 | None:intergenic | 40.0% | |
| AGTATTTGCTCTTCCCAAGT+GGG | + | chr6.3:29783938-29783957 | None:intergenic | 40.0% | |
| ATTGAGTAAGCTTCCCACTT+GGG | - | chr6.3:29783922-29783941 | MS.gene80426:CDS | 40.0% | |
| CATTGAGTAAGCTTCCCACT+TGG | - | chr6.3:29783921-29783940 | MS.gene80426:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 29783885 | 29784210 | 29783885 | ID=MS.gene80426 |
| chr6.3 | mRNA | 29783885 | 29784210 | 29783885 | ID=MS.gene80426.t1;Parent=MS.gene80426 |
| chr6.3 | exon | 29784074 | 29784210 | 29784074 | ID=MS.gene80426.t1.exon1;Parent=MS.gene80426.t1 |
| chr6.3 | CDS | 29784074 | 29784210 | 29784074 | ID=cds.MS.gene80426.t1;Parent=MS.gene80426.t1 |
| chr6.3 | exon | 29783885 | 29783950 | 29783885 | ID=MS.gene80426.t1.exon2;Parent=MS.gene80426.t1 |
| chr6.3 | CDS | 29783885 | 29783950 | 29783885 | ID=cds.MS.gene80426.t1;Parent=MS.gene80426.t1 |
| Gene Sequence |
| Protein sequence |
