Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80612.t1 | XP_003609088.1 | 97.4 | 152 | 4 | 0 | 1 | 152 | 1 | 152 | 2.10E-78 | 301.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80612.t1 | G7JT03 | 97.4 | 152 | 4 | 0 | 1 | 152 | 1 | 152 | 1.5e-78 | 301.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049181 | MS.gene80612 | 0.815812 | 7.63E-52 | -1.69E-46 |
| MS.gene056530 | MS.gene80612 | 0.812406 | 4.31E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80612.t1 | MTR_4g111840 | 97.368 | 152 | 4 | 0 | 1 | 152 | 1 | 152 | 2.21e-107 | 302 |
| MS.gene80612.t1 | MTR_4g077777 | 58.503 | 147 | 30 | 4 | 5 | 149 | 6 | 123 | 6.22e-47 | 149 |
| MS.gene80612.t1 | MTR_0077s0010 | 44.667 | 150 | 60 | 4 | 1 | 150 | 1 | 127 | 2.71e-32 | 112 |
| MS.gene80612.t1 | MTR_0077s0010 | 44.667 | 150 | 60 | 4 | 1 | 150 | 1 | 127 | 3.65e-32 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80612.t1 | AT1G49000 | 57.143 | 84 | 36 | 0 | 69 | 152 | 72 | 155 | 3.06e-29 | 105 |
| MS.gene80612.t1 | AT3G18560 | 49.000 | 100 | 39 | 2 | 65 | 152 | 82 | 181 | 5.05e-23 | 90.1 |
Find 44 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAAGCTCTGTCGCAATTC+GGG | 0.343880 | 8.4:+16586889 | MS.gene80612:CDS |
| AGGAGCGAGAGAAGGAAGGT+TGG | 0.352860 | 8.4:+16586928 | MS.gene80612:CDS |
| AACAAAGCTCTGTCGCAATT+CGG | 0.358109 | 8.4:+16586888 | MS.gene80612:CDS |
| GGAGCGAGAGAAGGAAGGTT+GGG | 0.363990 | 8.4:+16586929 | MS.gene80612:CDS |
| GTTGGGGAAACGGCGGCGTT+TGG | 0.367692 | 8.4:+16586946 | MS.gene80612:CDS |
| GGTTTCGCCATCGGTGATTT+CGG | 0.369181 | 8.4:-16586840 | None:intergenic |
| GAGAAGGAAGGTTGGGGAAA+CGG | 0.415587 | 8.4:+16586936 | MS.gene80612:CDS |
| TGATATACTACGATATTAAC+GGG | 0.426732 | 8.4:+16587021 | MS.gene80612:CDS |
| AACTTCTTCGGTTTCGCCAT+CGG | 0.447989 | 8.4:-16586849 | None:intergenic |
| CAACGGCGCGTGAGATATCC+AGG | 0.451264 | 8.4:-16587092 | None:intergenic |
| ATGTTGCTCAGCAACTTCTT+CGG | 0.460883 | 8.4:-16586861 | None:intergenic |
| GTGATATACTACGATATTAA+CGG | 0.474096 | 8.4:+16587020 | MS.gene80612:CDS |
| CCGTTGGAGTGGTGGCGGTT+CGG | 0.476183 | 8.4:-16586681 | None:intergenic |
| TGGTGGCGGTTCGGCGACTC+CGG | 0.488405 | 8.4:-16586672 | None:intergenic |
| AAACGGCGGCGTTTGGCAGA+AGG | 0.489679 | 8.4:+16586953 | MS.gene80612:CDS |
| AGCCGCCGTTGGAGTGGTGG+CGG | 0.497089 | 8.4:-16586686 | None:intergenic |
| GCGATTCTCGCTCTACTCTC+AGG | 0.498937 | 8.4:+16586726 | MS.gene80612:CDS |
| GAGCGAGAGAAGGAAGGTTG+GGG | 0.500115 | 8.4:+16586930 | MS.gene80612:CDS |
| TGTGAGCCGTTGGATTTCTC+CGG | 0.520015 | 8.4:+16586996 | MS.gene80612:CDS |
| CTCCACTCCTCGTCTCTGAT+CGG | 0.521441 | 8.4:-16586798 | None:intergenic |
| CCACGCGCTAGTCCTTTGCC+TGG | 0.524315 | 8.4:+16587074 | MS.gene80612:CDS |
| GCAGAGAGAGGAGCGAGAGA+AGG | 0.532332 | 8.4:+16586920 | MS.gene80612:CDS |
| AGACGCGTGAAGTTCCGATC+AGG | 0.541127 | 8.4:+16587048 | MS.gene80612:CDS |
| GGAAAAGCTAACCGATTGAA+AGG | 0.546588 | 8.4:+16586747 | MS.gene80612:CDS |
| AACCGCCACCACTCCAACGG+CGG | 0.547632 | 8.4:+16586684 | MS.gene80612:CDS |
| AATGGTGAAAATCACTTCGC+CGG | 0.550125 | 8.4:+16586653 | None:intergenic |
| AGAAGCCGCCGTTGGAGTGG+TGG | 0.555447 | 8.4:-16586689 | None:intergenic |
| TGTTATGAAGAAGCCGCCGT+TGG | 0.572522 | 8.4:-16586697 | None:intergenic |
| GGGAGGAAAGTGTGAGCCGT+TGG | 0.581512 | 8.4:+16586986 | MS.gene80612:CDS |
| CCGAACCGCCACCACTCCAA+CGG | 0.589190 | 8.4:+16586681 | MS.gene80612:CDS |
| TGAAGAAGCCGCCGTTGGAG+TGG | 0.592784 | 8.4:-16586692 | None:intergenic |
| CGCGTGAGATATCCAGGCAA+AGG | 0.600202 | 8.4:-16587086 | None:intergenic |
| CGATCAGAGACGAGGAGTGG+AGG | 0.603694 | 8.4:+16586799 | MS.gene80612:CDS |
| TTCGCCATCGGTGATTTCGG+CGG | 0.604781 | 8.4:-16586837 | None:intergenic |
| AGAGAGGAGCGAGAGAAGGA+AGG | 0.610746 | 8.4:+16586924 | MS.gene80612:CDS |
| CGGGAAGAAGAAGCAGAGAG+AGG | 0.622318 | 8.4:+16586908 | MS.gene80612:CDS |
| AACCGATCAGAGACGAGGAG+TGG | 0.623642 | 8.4:+16586796 | MS.gene80612:CDS |
| AAGGAAGGTTGGGGAAACGG+CGG | 0.630868 | 8.4:+16586939 | MS.gene80612:CDS |
| CTAGCGCGTGGAGACCTGAT+CGG | 0.633867 | 8.4:-16587062 | None:intergenic |
| CCAGGCAAAGGACTAGCGCG+TGG | 0.650067 | 8.4:-16587074 | None:intergenic |
| ATCACACCGGAGAAATCCAA+CGG | 0.664699 | 8.4:-16587002 | None:intergenic |
| GACGAAACCGATCAGAGACG+AGG | 0.672569 | 8.4:+16586791 | MS.gene80612:CDS |
| GACACCGCCGAAATCACCGA+TGG | 0.691978 | 8.4:+16586833 | MS.gene80612:CDS |
| AATATCGTAGTATATCACAC+CGG | 0.751710 | 8.4:-16587015 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GTGATATACTACGATATTAA+CGG | + | chr8.4:16587020-16587039 | MS.gene80612:CDS | 25.0% |
| ! | TGATATACTACGATATTAAC+GGG | + | chr8.4:16587021-16587040 | MS.gene80612:CDS | 25.0% |
| !!! | ATTTCGTTTTTCCTTTCAAT+CGG | - | chr8.4:16586761-16586780 | None:intergenic | 25.0% |
| AATATCGTAGTATATCACAC+CGG | - | chr8.4:16587018-16587037 | None:intergenic | 30.0% | |
| AACAAAGCTCTGTCGCAATT+CGG | + | chr8.4:16586888-16586907 | MS.gene80612:CDS | 40.0% | |
| ATGTTGCTCAGCAACTTCTT+CGG | - | chr8.4:16586864-16586883 | None:intergenic | 40.0% | |
| GGAAAAGCTAACCGATTGAA+AGG | + | chr8.4:16586747-16586766 | MS.gene80612:CDS | 40.0% | |
| ! | TTGGCAGAAGGAGATTTTGA+TGG | + | chr8.4:16586965-16586984 | MS.gene80612:CDS | 40.0% |
| !! | TGGCAGAAGGAGATTTTGAT+GGG | + | chr8.4:16586966-16586985 | MS.gene80612:CDS | 40.0% |
| AACTTCTTCGGTTTCGCCAT+CGG | - | chr8.4:16586852-16586871 | None:intergenic | 45.0% | |
| ACAAAGCTCTGTCGCAATTC+GGG | + | chr8.4:16586889-16586908 | MS.gene80612:CDS | 45.0% | |
| ATCACACCGGAGAAATCCAA+CGG | - | chr8.4:16587005-16587024 | None:intergenic | 45.0% | |
| !! | CAGAAGGAGATTTTGATGGG+AGG | + | chr8.4:16586969-16586988 | MS.gene80612:CDS | 45.0% |
| GAGAAGGAAGGTTGGGGAAA+CGG | + | chr8.4:16586936-16586955 | MS.gene80612:CDS | 50.0% | |
| GGTTTCGCCATCGGTGATTT+CGG | - | chr8.4:16586843-16586862 | None:intergenic | 50.0% | |
| TGTGAGCCGTTGGATTTCTC+CGG | + | chr8.4:16586996-16587015 | MS.gene80612:CDS | 50.0% | |
| TGTTATGAAGAAGCCGCCGT+TGG | - | chr8.4:16586700-16586719 | None:intergenic | 50.0% | |
| AACCGATCAGAGACGAGGAG+TGG | + | chr8.4:16586796-16586815 | MS.gene80612:CDS | 55.0% | |
| AAGGAAGGTTGGGGAAACGG+CGG | + | chr8.4:16586939-16586958 | MS.gene80612:CDS | 55.0% | |
| AGACGCGTGAAGTTCCGATC+AGG | + | chr8.4:16587048-16587067 | MS.gene80612:CDS | 55.0% | |
| AGAGAGGAGCGAGAGAAGGA+AGG | + | chr8.4:16586924-16586943 | MS.gene80612:CDS | 55.0% | |
| AGGAGCGAGAGAAGGAAGGT+TGG | + | chr8.4:16586928-16586947 | MS.gene80612:CDS | 55.0% | |
| CGCGTGAGATATCCAGGCAA+AGG | - | chr8.4:16587089-16587108 | None:intergenic | 55.0% | |
| CGGGAAGAAGAAGCAGAGAG+AGG | + | chr8.4:16586908-16586927 | MS.gene80612:CDS | 55.0% | |
| CTCCACTCCTCGTCTCTGAT+CGG | - | chr8.4:16586801-16586820 | None:intergenic | 55.0% | |
| GACGAAACCGATCAGAGACG+AGG | + | chr8.4:16586791-16586810 | MS.gene80612:CDS | 55.0% | |
| GAGCGAGAGAAGGAAGGTTG+GGG | + | chr8.4:16586930-16586949 | MS.gene80612:CDS | 55.0% | |
| GCGATTCTCGCTCTACTCTC+AGG | + | chr8.4:16586726-16586745 | MS.gene80612:CDS | 55.0% | |
| GGAGCGAGAGAAGGAAGGTT+GGG | + | chr8.4:16586929-16586948 | MS.gene80612:CDS | 55.0% | |
| TTCGCCATCGGTGATTTCGG+CGG | - | chr8.4:16586840-16586859 | None:intergenic | 55.0% | |
| AAACGGCGGCGTTTGGCAGA+AGG | + | chr8.4:16586953-16586972 | MS.gene80612:CDS | 60.0% | |
| CAACGGCGCGTGAGATATCC+AGG | - | chr8.4:16587095-16587114 | None:intergenic | 60.0% | |
| CGATCAGAGACGAGGAGTGG+AGG | + | chr8.4:16586799-16586818 | MS.gene80612:CDS | 60.0% | |
| CTAGCGCGTGGAGACCTGAT+CGG | - | chr8.4:16587065-16587084 | None:intergenic | 60.0% | |
| GACACCGCCGAAATCACCGA+TGG | + | chr8.4:16586833-16586852 | MS.gene80612:CDS | 60.0% | |
| GCAGAGAGAGGAGCGAGAGA+AGG | + | chr8.4:16586920-16586939 | MS.gene80612:CDS | 60.0% | |
| TGAAGAAGCCGCCGTTGGAG+TGG | - | chr8.4:16586695-16586714 | None:intergenic | 60.0% | |
| ! | GGGAGGAAAGTGTGAGCCGT+TGG | + | chr8.4:16586986-16587005 | MS.gene80612:CDS | 60.0% |
| AACCGCCACCACTCCAACGG+CGG | + | chr8.4:16586684-16586703 | MS.gene80612:CDS | 65.0% | |
| AGAAGCCGCCGTTGGAGTGG+TGG | - | chr8.4:16586692-16586711 | None:intergenic | 65.0% | |
| CCACGCGCTAGTCCTTTGCC+TGG | + | chr8.4:16587074-16587093 | MS.gene80612:CDS | 65.0% | |
| CCGAACCGCCACCACTCCAA+CGG | + | chr8.4:16586681-16586700 | MS.gene80612:CDS | 65.0% | |
| GTTGGGGAAACGGCGGCGTT+TGG | + | chr8.4:16586946-16586965 | MS.gene80612:CDS | 65.0% | |
| ! | CCAGGCAAAGGACTAGCGCG+TGG | - | chr8.4:16587077-16587096 | None:intergenic | 65.0% |
| !! | CCGTTGGAGTGGTGGCGGTT+CGG | - | chr8.4:16586684-16586703 | None:intergenic | 65.0% |
| AGCCGCCGTTGGAGTGGTGG+CGG | - | chr8.4:16586689-16586708 | None:intergenic | 70.0% | |
| !! | TGGTGGCGGTTCGGCGACTC+CGG | - | chr8.4:16586675-16586694 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 16586654 | 16587115 | 16586654 | ID=MS.gene80612 |
| chr8.4 | mRNA | 16586654 | 16587115 | 16586654 | ID=MS.gene80612.t1;Parent=MS.gene80612 |
| chr8.4 | exon | 16586654 | 16587115 | 16586654 | ID=MS.gene80612.t1.exon1;Parent=MS.gene80612.t1 |
| chr8.4 | CDS | 16586654 | 16587115 | 16586654 | ID=cds.MS.gene80612.t1;Parent=MS.gene80612.t1 |
| Gene Sequence |
| Protein sequence |
