AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene049181


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049181.t1 MTR_3g098390 90.741 108 1 2 1 108 1 99 3.74e-66 194
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049181.t1 AT2G44010 35.345 116 50 5 1 107 1 100 4.39e-12 57.8

Find 16 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGTATGAGGTTATTTCTTT+AGG 0.254934 3.3:-80936502 MS.gene049181:CDS
AAATCTGTTGGAGATAGTTT+TGG 0.346900 3.3:-80936370 MS.gene049181:CDS
GCTTCAGATAATTACAATAT+TGG 0.370686 3.3:-80936406 MS.gene049181:CDS
TTTCATGATCAGACAACAAC+TGG 0.416618 3.3:-80936334 MS.gene049181:CDS
TAGAACAAGACGACAGTATG+AGG 0.483439 3.3:-80936515 MS.gene049181:CDS
TGAAGTGGTTGTTGAGTTTG+AGG 0.489679 3.3:+80936445 None:intergenic
CACAGGAGACATAAATCTGT+TGG 0.502696 3.3:-80936382 MS.gene049181:CDS
TATGAGGTTATTTCTTTAGG+AGG 0.514646 3.3:-80936499 MS.gene049181:CDS
GTGGTTGTTGAGTTTGAGGG+TGG 0.516517 3.3:+80936449 None:intergenic
GAAGTGGTTGTTGAGTTTGA+GGG 0.525781 3.3:+80936446 None:intergenic
ATATTGTAATTATCTGAAGC+TGG 0.533222 3.3:+80936409 None:intergenic
TTGATTGCTTTATACACAAG+AGG 0.566790 3.3:+80936544 None:intergenic
ATAATTACAATATTGGTCAC+AGG 0.578276 3.3:-80936399 MS.gene049181:CDS
GGTATTTGATTATGATGAAG+TGG 0.585229 3.3:+80936430 None:intergenic
ACAATAAGAAGTGTTATGGA+AGG 0.606553 3.3:-80936574 None:intergenic
AGGAAGCTACAACATAAGCA+TGG 0.662553 3.3:-80936479 MS.gene049181:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACAAGTTTTTTTGAAGAATT+AGG + chr3.3:80936516-80936535 None:intergenic 20.0%
! ATAATTACAATATTGGTCAC+AGG - chr3.3:80936415-80936434 MS.gene049181:CDS 25.0%
! ATTCTTCAAAAAAACTTGTC+AGG - chr3.3:80936517-80936536 MS.gene049181:CDS 25.0%
! GCTTCAGATAATTACAATAT+TGG - chr3.3:80936408-80936427 MS.gene049181:CDS 25.0%
!! AGAATGTTTTCATGTATCAA+GGG - chr3.3:80936552-80936571 MS.gene049181:CDS 25.0%
!! ATATTGTAATTATCTGAAGC+TGG + chr3.3:80936408-80936427 None:intergenic 25.0%
!! TAGAATGTTTTCATGTATCA+AGG - chr3.3:80936551-80936570 MS.gene049181:CDS 25.0%
GGTATTTGATTATGATGAAG+TGG + chr3.3:80936387-80936406 None:intergenic 30.0%
TTGATTGCTTTATACACAAG+AGG + chr3.3:80936273-80936292 None:intergenic 30.0%
! AAATCTGTTGGAGATAGTTT+TGG - chr3.3:80936444-80936463 MS.gene049181:CDS 30.0%
!! CAGTATGAGGTTATTTCTTT+AGG - chr3.3:80936312-80936331 MS.gene049181:CDS 30.0%
!! TATGAGGTTATTTCTTTAGG+AGG - chr3.3:80936315-80936334 MS.gene049181:CDS 30.0%
TTTCATGATCAGACAACAAC+TGG - chr3.3:80936480-80936499 MS.gene049181:CDS 35.0%
!!! GTTGGAGATAGTTTTGGTAA+TGG - chr3.3:80936450-80936469 MS.gene049181:CDS 35.0%
AGGAAGCTACAACATAAGCA+TGG - chr3.3:80936335-80936354 MS.gene049181:CDS 40.0%
CACAGGAGACATAAATCTGT+TGG - chr3.3:80936432-80936451 MS.gene049181:CDS 40.0%
GAAGTGGTTGTTGAGTTTGA+GGG + chr3.3:80936371-80936390 None:intergenic 40.0%
TAGAACAAGACGACAGTATG+AGG - chr3.3:80936299-80936318 MS.gene049181:CDS 40.0%
TGAAGTGGTTGTTGAGTTTG+AGG + chr3.3:80936372-80936391 None:intergenic 40.0%
GTGGTTGTTGAGTTTGAGGG+TGG + chr3.3:80936368-80936387 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 80936255 80936581 80936255 ID=MS.gene049181
chr3.3 mRNA 80936255 80936581 80936255 ID=MS.gene049181.t1;Parent=MS.gene049181
chr3.3 exon 80936255 80936581 80936255 ID=MS.gene049181.t1.exon1;Parent=MS.gene049181.t1
chr3.3 CDS 80936255 80936581 80936255 ID=cds.MS.gene049181.t1;Parent=MS.gene049181.t1
Gene Sequence

>MS.gene049181

ATGGAAGGTCTTCTACCTCTTGTGTATAAAGCAATCAAGAAAAATAGAACAAGACGACAGTATGAGGTTATTTCTTTAGGAGGAAGCTACAACATAAGCATGGCTGAGATCCACCCTCAAACTCAACAACCACTTCATCATAATCAAATACCAGCTTCAGATAATTACAATATTGGTCACAGGAGACATAAATCTGTTGGAGATAGTTTTGGTAATGGTTTTCAATTTCATGATCAGACAACAACTGGTTCTGTTTCTCCTAATTCTTCAAAAAAACTTGTCAGGTTTAGTAGTCATAGAATGTTTTCATGTATCAAGGGTGCCTGA

Protein sequence

>MS.gene049181.t1

MEGLLPLVYKAIKKNRTRRQYEVISLGGSYNISMAEIHPQTQQPLHHNQIPASDNYNIGHRRHKSVGDSFGNGFQFHDQTTTGSVSPNSSKKLVRFSSHRMFSCIKGA