Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80709.t1 | XP_003613209.1 | 99.5 | 217 | 1 | 0 | 1 | 217 | 1 | 217 | 1.80E-107 | 398.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80709.t1 | Q9LDT3 | 56.4 | 227 | 84 | 5 | 2 | 217 | 9 | 231 | 3.9e-47 | 189.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80709.t1 | G7K086 | 99.5 | 217 | 1 | 0 | 1 | 217 | 1 | 217 | 1.3e-107 | 398.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene80709.t1 | TF | C2C2-YABBY |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053074 | MS.gene80709 | 0.805982 | 1.03E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80709.t1 | MTR_5g034030 | 99.539 | 217 | 1 | 0 | 1 | 217 | 1 | 217 | 6.88e-164 | 451 |
| MS.gene80709.t1 | MTR_4g114730 | 49.686 | 159 | 58 | 4 | 12 | 162 | 14 | 158 | 1.12e-44 | 147 |
| MS.gene80709.t1 | MTR_2g087740 | 51.250 | 160 | 62 | 4 | 12 | 162 | 12 | 164 | 6.68e-44 | 145 |
| MS.gene80709.t1 | MTR_4g050300 | 48.344 | 151 | 73 | 1 | 12 | 162 | 9 | 154 | 5.85e-41 | 137 |
| MS.gene80709.t1 | MTR_4g025900 | 50.000 | 156 | 70 | 2 | 14 | 162 | 25 | 179 | 6.93e-40 | 136 |
| MS.gene80709.t1 | MTR_4g101660 | 51.553 | 161 | 64 | 5 | 12 | 162 | 18 | 174 | 2.23e-39 | 135 |
| MS.gene80709.t1 | MTR_5g046230 | 34.021 | 194 | 99 | 3 | 12 | 195 | 17 | 191 | 1.45e-29 | 109 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80709.t1 | AT1G23420 | 55.947 | 227 | 85 | 4 | 2 | 217 | 9 | 231 | 4.21e-73 | 221 |
| MS.gene80709.t1 | AT1G23420 | 55.947 | 227 | 85 | 4 | 2 | 217 | 40 | 262 | 5.59e-73 | 222 |
| MS.gene80709.t1 | AT2G26580 | 50.327 | 153 | 60 | 3 | 12 | 162 | 9 | 147 | 2.67e-46 | 151 |
| MS.gene80709.t1 | AT2G26580 | 50.327 | 153 | 60 | 3 | 12 | 162 | 9 | 147 | 2.67e-46 | 151 |
| MS.gene80709.t1 | AT2G45190 | 46.061 | 165 | 79 | 1 | 8 | 162 | 19 | 183 | 1.01e-42 | 144 |
| MS.gene80709.t1 | AT1G08465 | 49.677 | 155 | 66 | 4 | 12 | 162 | 8 | 154 | 7.40e-41 | 137 |
| MS.gene80709.t1 | AT4G00180 | 45.810 | 179 | 71 | 5 | 8 | 162 | 19 | 195 | 8.09e-39 | 134 |
| MS.gene80709.t1 | AT4G00180 | 43.976 | 166 | 67 | 5 | 21 | 162 | 1 | 164 | 6.41e-30 | 110 |
| MS.gene80709.t1 | AT1G69180 | 40.385 | 156 | 73 | 4 | 12 | 163 | 19 | 158 | 5.82e-29 | 107 |
| MS.gene80709.t1 | AT2G45190 | 63.636 | 66 | 24 | 0 | 97 | 162 | 54 | 119 | 3.98e-25 | 97.1 |
Find 43 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGCAGAGAAGAGATTAAA+AGG | 0.220244 | 5.4:+24013892 | MS.gene80709:intron |
| CATCACCTTTGCACTGAGTT+GGG | 0.247070 | 5.4:-24014157 | None:intergenic |
| GCTTCCTTGTGAGCCATATC+AGG | 0.254990 | 5.4:-24013930 | None:intergenic |
| ACTCATGGTTACCATTAAAA+TGG | 0.296363 | 5.4:-24013154 | None:intergenic |
| CTTTCCAAGATGGAATTTCT+TGG | 0.337983 | 5.4:-24014850 | None:intergenic |
| GTCTTGTTTGCATCTTCATC+TGG | 0.344790 | 5.4:-24013552 | None:intergenic |
| GATGAGTAAGAGATGCTAAA+AGG | 0.346967 | 5.4:-24013388 | None:intergenic |
| GGTGTTCTTTGTCTCTTCTC+TGG | 0.372695 | 5.4:-24013759 | None:intergenic |
| TGTACATAGCAAATCTGTTC+TGG | 0.376697 | 5.4:-24013114 | None:intergenic |
| TCATCACCTTTGCACTGAGT+TGG | 0.384038 | 5.4:-24014158 | None:intergenic |
| TATTCATTCATTCACTCAAA+TGG | 0.405002 | 5.4:-24014877 | None:intergenic |
| TTATTGACTACATTGCTGAT+TGG | 0.408933 | 5.4:-24013639 | None:intergenic |
| CAGCTTCATGAGGGTCCACT+TGG | 0.437038 | 5.4:-24014223 | None:intergenic |
| ATCACCTTTGCACTGAGTTG+GGG | 0.440172 | 5.4:-24014156 | None:intergenic |
| GATCCACAAATTGATCTGTC+TGG | 0.455209 | 5.4:-24014193 | None:intergenic |
| ACAAGGATTCCGTGGAAGAA+AGG | 0.512794 | 5.4:+24014825 | MS.gene80709:CDS |
| AGCATCTCTTACTCATCTTG+AGG | 0.521370 | 5.4:+24013394 | MS.gene80709:CDS |
| GGTACCAAGAAATTCCATCT+TGG | 0.522438 | 5.4:+24014846 | MS.gene80709:CDS |
| AGCTTCATGAGGGTCCACTT+GGG | 0.522893 | 5.4:-24014222 | None:intergenic |
| ACAATCAGAATAAGTCATCA+TGG | 0.529221 | 5.4:-24013598 | None:intergenic |
| AAATGGTGTCCTTTCCAAGA+TGG | 0.529231 | 5.4:-24014860 | None:intergenic |
| TAGCCAGACAGATCAATTTG+TGG | 0.529249 | 5.4:+24014190 | MS.gene80709:CDS |
| AGAAGAGATTAAAAGGCTAA+AGG | 0.529887 | 5.4:+24013899 | MS.gene80709:CDS |
| ATATTAATTACTTGATGAAG+CGG | 0.533614 | 5.4:-24013794 | None:intergenic |
| TTCTTGGTACCTTTCTTCCA+CGG | 0.541076 | 5.4:-24014834 | None:intergenic |
| CTTCCTTGTGAGCCATATCA+GGG | 0.545406 | 5.4:-24013929 | None:intergenic |
| GAGATGCTAAAAGGTGGAAA+GGG | 0.553103 | 5.4:-24013379 | None:intergenic |
| AGAGATGCTAAAAGGTGGAA+AGG | 0.557063 | 5.4:-24013380 | None:intergenic |
| GAGTAAGAGATGCTAAAAGG+TGG | 0.562368 | 5.4:-24013385 | None:intergenic |
| ACCATTGTCAAACTGCTGCA+TGG | 0.563063 | 5.4:-24013290 | None:intergenic |
| CAGGTGAATGAAGAAGAACA+AGG | 0.564909 | 5.4:+24014808 | MS.gene80709:intron |
| GAAGAAGAACAAGGATTCCG+TGG | 0.572480 | 5.4:+24014817 | MS.gene80709:CDS |
| AAACCCTGATATGGCTCACA+AGG | 0.577441 | 5.4:+24013926 | MS.gene80709:CDS |
| CAAGAAATTCCATCTTGGAA+AGG | 0.597804 | 5.4:+24014851 | MS.gene80709:CDS |
| TCACCTTTGCACTGAGTTGG+GGG | 0.601505 | 5.4:-24014155 | None:intergenic |
| GATGACTTATTCTGATTGTG+AGG | 0.620697 | 5.4:+24013602 | MS.gene80709:CDS |
| CAGATTTGCTATGTACAATG+TGG | 0.632418 | 5.4:+24013120 | MS.gene80709:CDS |
| AGTGGACCCTCATGAAGCTG+AGG | 0.650640 | 5.4:+24014226 | MS.gene80709:CDS |
| TCCATGCAGCAGTTTGACAA+TGG | 0.655787 | 5.4:+24013289 | MS.gene80709:CDS |
| ATGAAGCGGTTATAAGCTGA+TGG | 0.663979 | 5.4:-24013780 | None:intergenic |
| TGTGGATCTTGACTCCCAAG+TGG | 0.667148 | 5.4:+24014208 | MS.gene80709:CDS |
| ATGCAGCAGTTTGACAATGG+TGG | 0.669140 | 5.4:+24013292 | MS.gene80709:CDS |
| ATGGTGGTGACAGTAAGATG+TGG | 0.764759 | 5.4:+24013308 | MS.gene80709:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTATTTTTAATTTTCAGT+GGG | + | chr5.4:24014124-24014143 | MS.gene80709:intron | 10.0% |
| !!! | TTTTTATTTTTAATTTTCAG+TGG | + | chr5.4:24014123-24014142 | MS.gene80709:intron | 10.0% |
| !! | AAAGAAAATAAACAAAGTAT+TGG | - | chr5.4:24013442-24013461 | None:intergenic | 15.0% |
| !!! | CAAAGCATAATATTTTTAAA+AGG | - | chr5.4:24014591-24014610 | None:intergenic | 15.0% |
| !!! | TATAGGAAATTTTATAAGAT+TGG | + | chr5.4:24014063-24014082 | MS.gene80709:intron | 15.0% |
| !!! | TTGTTTATTTTCTTTTAGTT+AGG | + | chr5.4:24013447-24013466 | MS.gene80709:intron | 15.0% |
| !! | ATATTAATTACTTGATGAAG+CGG | - | chr5.4:24013797-24013816 | None:intergenic | 20.0% |
| ! | CTTCTTAACTTCATCATATT+TGG | + | chr5.4:24013189-24013208 | MS.gene80709:intron | 25.0% |
| ! | TCAACTCATTTGAAAACTAT+AGG | + | chr5.4:24014046-24014065 | MS.gene80709:intron | 25.0% |
| !! | ATTCTGCAATACCATTTTAA+TGG | + | chr5.4:24013143-24013162 | MS.gene80709:CDS | 25.0% |
| !!! | CATTAAAACTTGCTATAAGT+AGG | + | chr5.4:24014344-24014363 | MS.gene80709:intron | 25.0% |
| AAGAGCAATTAGAAAACTCA+TGG | - | chr5.4:24013172-24013191 | None:intergenic | 30.0% | |
| AATGCAGAGAAGAGATTAAA+AGG | + | chr5.4:24013892-24013911 | MS.gene80709:intron | 30.0% | |
| ACAATCAGAATAAGTCATCA+TGG | - | chr5.4:24013601-24013620 | None:intergenic | 30.0% | |
| ACTCATGGTTACCATTAAAA+TGG | - | chr5.4:24013157-24013176 | None:intergenic | 30.0% | |
| ACTTTATATCCTCATCTTGT+TGG | + | chr5.4:24014472-24014491 | MS.gene80709:intron | 30.0% | |
| AGAAGAGATTAAAAGGCTAA+AGG | + | chr5.4:24013899-24013918 | MS.gene80709:CDS | 30.0% | |
| AGTAGGAGATTCTTTAGATT+TGG | + | chr5.4:24014361-24014380 | MS.gene80709:intron | 30.0% | |
| GTCTCTTTCTTTATATCACA+TGG | + | chr5.4:24014410-24014429 | MS.gene80709:intron | 30.0% | |
| TAAAAGGCTATGTCAAATGA+CGG | - | chr5.4:24014575-24014594 | None:intergenic | 30.0% | |
| TATAGATCACAAAAACAGGT+AGG | - | chr5.4:24014749-24014768 | None:intergenic | 30.0% | |
| TATCACATGGTGAATAGTAA+AGG | + | chr5.4:24014423-24014442 | MS.gene80709:intron | 30.0% | |
| ! | AAAACAGGTAGGTTTTTCTT+TGG | - | chr5.4:24014738-24014757 | None:intergenic | 30.0% |
| ! | TTATTGACTACATTGCTGAT+TGG | - | chr5.4:24013642-24013661 | None:intergenic | 30.0% |
| !!! | TAGCTAGTTAGTTTTGAACA+TGG | - | chr5.4:24014021-24014040 | None:intergenic | 30.0% |
| AAAGGCTAAAAACCCTGATA+TGG | + | chr5.4:24013917-24013936 | MS.gene80709:CDS | 35.0% | |
| CAAGAAATTCCATCTTGGAA+AGG | + | chr5.4:24014851-24014870 | MS.gene80709:CDS | 35.0% | |
| CTGCTATAGATCACAAAAAC+AGG | - | chr5.4:24014753-24014772 | None:intergenic | 35.0% | |
| CTTTCCAAGATGGAATTTCT+TGG | - | chr5.4:24014853-24014872 | None:intergenic | 35.0% | |
| GATGACTTATTCTGATTGTG+AGG | + | chr5.4:24013602-24013621 | MS.gene80709:CDS | 35.0% | |
| GATGAGTAAGAGATGCTAAA+AGG | - | chr5.4:24013391-24013410 | None:intergenic | 35.0% | |
| GTACAGCTGCAAAAAATGTA+AGG | + | chr5.4:24013958-24013977 | MS.gene80709:intron | 35.0% | |
| TCATTTCAATTGTGTGATGC+AGG | + | chr5.4:24013259-24013278 | MS.gene80709:intron | 35.0% | |
| TGGTGACTTACTCATAATCT+TGG | + | chr5.4:24014083-24014102 | MS.gene80709:intron | 35.0% | |
| TGTACATAGCAAATCTGTTC+TGG | - | chr5.4:24013117-24013136 | None:intergenic | 35.0% | |
| ! | TTTTTACCTCAGCTTCATGA+GGG | - | chr5.4:24014235-24014254 | None:intergenic | 35.0% |
| !! | CAGATTTGCTATGTACAATG+TGG | + | chr5.4:24013120-24013139 | MS.gene80709:CDS | 35.0% |
| !! | GTTCTGGAAGATCAAAAAGA+TGG | - | chr5.4:24013101-24013120 | None:intergenic | 35.0% |
| AAATGGTGTCCTTTCCAAGA+TGG | - | chr5.4:24014863-24014882 | None:intergenic | 40.0% | |
| AGAGATGCTAAAAGGTGGAA+AGG | - | chr5.4:24013383-24013402 | None:intergenic | 40.0% | |
| AGCATCTCTTACTCATCTTG+AGG | + | chr5.4:24013394-24013413 | MS.gene80709:CDS | 40.0% | |
| AGTCTTTGCCATTGAATGCA+TGG | - | chr5.4:24014455-24014474 | None:intergenic | 40.0% | |
| ATGAAGCGGTTATAAGCTGA+TGG | - | chr5.4:24013783-24013802 | None:intergenic | 40.0% | |
| ATGACATTGATCCACCAAAC+TGG | + | chr5.4:24014507-24014526 | MS.gene80709:intron | 40.0% | |
| CAGGTGAATGAAGAAGAACA+AGG | + | chr5.4:24014808-24014827 | MS.gene80709:intron | 40.0% | |
| GAGATGCTAAAAGGTGGAAA+GGG | - | chr5.4:24013382-24013401 | None:intergenic | 40.0% | |
| GAGTAAGAGATGCTAAAAGG+TGG | - | chr5.4:24013388-24013407 | None:intergenic | 40.0% | |
| GATCCACAAATTGATCTGTC+TGG | - | chr5.4:24014196-24014215 | None:intergenic | 40.0% | |
| GATTGGAAACCAACAAGATG+AGG | - | chr5.4:24014484-24014503 | None:intergenic | 40.0% | |
| GGTACCAAGAAATTCCATCT+TGG | + | chr5.4:24014846-24014865 | MS.gene80709:CDS | 40.0% | |
| GGTATAGTCCATGCATTCAA+TGG | + | chr5.4:24014444-24014463 | MS.gene80709:intron | 40.0% | |
| TAGCCAGACAGATCAATTTG+TGG | + | chr5.4:24014190-24014209 | MS.gene80709:CDS | 40.0% | |
| TCTGCAAGAACTTTCCAGTT+TGG | - | chr5.4:24014524-24014543 | None:intergenic | 40.0% | |
| TGGATCAATGTCATGCTGAT+TGG | - | chr5.4:24014501-24014520 | None:intergenic | 40.0% | |
| TTCTTGGTACCTTTCTTCCA+CGG | - | chr5.4:24014837-24014856 | None:intergenic | 40.0% | |
| ! | GTTTTTACCTCAGCTTCATG+AGG | - | chr5.4:24014236-24014255 | None:intergenic | 40.0% |
| !! | GAACTGCATCATCACTTTTC+AGG | + | chr5.4:24014789-24014808 | MS.gene80709:intron | 40.0% |
| !! | GTCTTGTTTGCATCTTCATC+TGG | - | chr5.4:24013555-24013574 | None:intergenic | 40.0% |
| AAACCCTGATATGGCTCACA+AGG | + | chr5.4:24013926-24013945 | MS.gene80709:CDS | 45.0% | |
| ACAAGGATTCCGTGGAAGAA+AGG | + | chr5.4:24014825-24014844 | MS.gene80709:CDS | 45.0% | |
| ACCATTGTCAAACTGCTGCA+TGG | - | chr5.4:24013293-24013312 | None:intergenic | 45.0% | |
| ATCACCTTTGCACTGAGTTG+GGG | - | chr5.4:24014159-24014178 | None:intergenic | 45.0% | |
| ATGCAGCAGTTTGACAATGG+TGG | + | chr5.4:24013292-24013311 | MS.gene80709:CDS | 45.0% | |
| CATCACCTTTGCACTGAGTT+GGG | - | chr5.4:24014160-24014179 | None:intergenic | 45.0% | |
| CTTCCTTGTGAGCCATATCA+GGG | - | chr5.4:24013932-24013951 | None:intergenic | 45.0% | |
| GAAGAAGAACAAGGATTCCG+TGG | + | chr5.4:24014817-24014836 | MS.gene80709:CDS | 45.0% | |
| GCAAGAACTTTCCAGTTTGG+TGG | - | chr5.4:24014521-24014540 | None:intergenic | 45.0% | |
| GGCACTGTGTGCATTTATAG+TGG | + | chr5.4:24014382-24014401 | MS.gene80709:intron | 45.0% | |
| GGTGTTCTTTGTCTCTTCTC+TGG | - | chr5.4:24013762-24013781 | None:intergenic | 45.0% | |
| TCATCACCTTTGCACTGAGT+TGG | - | chr5.4:24014161-24014180 | None:intergenic | 45.0% | |
| TCCATGCAGCAGTTTGACAA+TGG | + | chr5.4:24013289-24013308 | MS.gene80709:CDS | 45.0% | |
| ! | ATGGTGGTGACAGTAAGATG+TGG | + | chr5.4:24013308-24013327 | MS.gene80709:CDS | 45.0% |
| AGCTTCATGAGGGTCCACTT+GGG | - | chr5.4:24014225-24014244 | None:intergenic | 50.0% | |
| GCTTCCTTGTGAGCCATATC+AGG | - | chr5.4:24013933-24013952 | None:intergenic | 50.0% | |
| TGTGGATCTTGACTCCCAAG+TGG | + | chr5.4:24014208-24014227 | MS.gene80709:CDS | 50.0% | |
| ! | TCACCTTTGCACTGAGTTGG+GGG | - | chr5.4:24014158-24014177 | None:intergenic | 50.0% |
| AGTGGACCCTCATGAAGCTG+AGG | + | chr5.4:24014226-24014245 | MS.gene80709:CDS | 55.0% | |
| CAGCTTCATGAGGGTCCACT+TGG | - | chr5.4:24014226-24014245 | None:intergenic | 55.0% | |
| TGTCCCCCAACTCAGTGCAA+AGG | + | chr5.4:24014152-24014171 | MS.gene80709:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 24013084 | 24014888 | 24013084 | ID=MS.gene80709 |
| chr5.4 | mRNA | 24013084 | 24014888 | 24013084 | ID=MS.gene80709.t1;Parent=MS.gene80709 |
| chr5.4 | exon | 24013084 | 24013164 | 24013084 | ID=MS.gene80709.t1.exon1;Parent=MS.gene80709.t1 |
| chr5.4 | CDS | 24013084 | 24013164 | 24013084 | ID=cds.MS.gene80709.t1;Parent=MS.gene80709.t1 |
| chr5.4 | exon | 24013281 | 24013415 | 24013281 | ID=MS.gene80709.t1.exon2;Parent=MS.gene80709.t1 |
| chr5.4 | CDS | 24013281 | 24013415 | 24013281 | ID=cds.MS.gene80709.t1;Parent=MS.gene80709.t1 |
| chr5.4 | exon | 24013534 | 24013663 | 24013534 | ID=MS.gene80709.t1.exon3;Parent=MS.gene80709.t1 |
| chr5.4 | CDS | 24013534 | 24013663 | 24013534 | ID=cds.MS.gene80709.t1;Parent=MS.gene80709.t1 |
| chr5.4 | exon | 24013757 | 24013805 | 24013757 | ID=MS.gene80709.t1.exon4;Parent=MS.gene80709.t1 |
| chr5.4 | CDS | 24013757 | 24013805 | 24013757 | ID=cds.MS.gene80709.t1;Parent=MS.gene80709.t1 |
| chr5.4 | exon | 24013899 | 24013974 | 24013899 | ID=MS.gene80709.t1.exon5;Parent=MS.gene80709.t1 |
| chr5.4 | CDS | 24013899 | 24013974 | 24013899 | ID=cds.MS.gene80709.t1;Parent=MS.gene80709.t1 |
| chr5.4 | exon | 24014143 | 24014247 | 24014143 | ID=MS.gene80709.t1.exon6;Parent=MS.gene80709.t1 |
| chr5.4 | CDS | 24014143 | 24014247 | 24014143 | ID=cds.MS.gene80709.t1;Parent=MS.gene80709.t1 |
| chr5.4 | exon | 24014811 | 24014888 | 24014811 | ID=MS.gene80709.t1.exon7;Parent=MS.gene80709.t1 |
| chr5.4 | CDS | 24014811 | 24014888 | 24014811 | ID=cds.MS.gene80709.t1;Parent=MS.gene80709.t1 |
| Gene Sequence |
| Protein sequence |
