Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80967.t1 | XP_013447451.1 | 89.1 | 55 | 6 | 0 | 1 | 55 | 127 | 181 | 2.10E-20 | 107.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80967.t1 | P00869 | 83.6 | 55 | 9 | 0 | 1 | 55 | 126 | 180 | 2.1e-21 | 102.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80967.t1 | A0A072U6Q1 | 89.1 | 55 | 6 | 0 | 1 | 55 | 127 | 181 | 1.5e-20 | 107.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049173 | MS.gene80967 | 0.805258 | 1.46E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80967.t1 | MTR_7g007120 | 89.091 | 55 | 6 | 0 | 1 | 55 | 127 | 181 | 8.32e-33 | 110 |
| MS.gene80967.t1 | MTR_7g007120 | 87.273 | 55 | 7 | 0 | 1 | 55 | 127 | 181 | 3.39e-32 | 108 |
| MS.gene80967.t1 | MTR_7g007120 | 87.273 | 55 | 7 | 0 | 1 | 55 | 127 | 181 | 3.62e-32 | 108 |
| MS.gene80967.t1 | MTR_7g007200 | 87.273 | 55 | 7 | 0 | 1 | 55 | 127 | 181 | 3.74e-32 | 108 |
| MS.gene80967.t1 | MTR_7g007220 | 87.273 | 55 | 7 | 0 | 1 | 55 | 127 | 181 | 6.51e-32 | 108 |
| MS.gene80967.t1 | MTR_7g007230 | 85.455 | 55 | 8 | 0 | 1 | 55 | 127 | 181 | 1.16e-30 | 105 |
| MS.gene80967.t1 | MTR_6g018300 | 83.636 | 55 | 9 | 0 | 1 | 55 | 123 | 177 | 1.17e-30 | 105 |
| MS.gene80967.t1 | MTR_6g018310 | 83.636 | 55 | 9 | 0 | 1 | 55 | 126 | 180 | 2.82e-30 | 104 |
| MS.gene80967.t1 | MTR_7g007210 | 84.211 | 38 | 6 | 0 | 1 | 38 | 127 | 164 | 4.37e-19 | 75.9 |
| MS.gene80967.t1 | MTR_4g127870 | 44.231 | 52 | 29 | 0 | 1 | 52 | 116 | 167 | 9.51e-16 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80967.t1 | AT1G67090 | 65.455 | 55 | 19 | 0 | 1 | 55 | 124 | 178 | 1.03e-23 | 87.4 |
| MS.gene80967.t1 | AT5G38420 | 65.455 | 55 | 19 | 0 | 1 | 55 | 124 | 178 | 3.28e-23 | 86.3 |
| MS.gene80967.t1 | AT5G38430 | 65.455 | 55 | 19 | 0 | 1 | 55 | 124 | 178 | 3.77e-23 | 85.9 |
| MS.gene80967.t1 | AT5G38430 | 65.455 | 55 | 19 | 0 | 1 | 55 | 124 | 178 | 3.77e-23 | 85.9 |
| MS.gene80967.t1 | AT5G38410 | 65.455 | 55 | 19 | 0 | 1 | 55 | 129 | 183 | 4.36e-23 | 85.9 |
| MS.gene80967.t1 | AT5G38410 | 65.455 | 55 | 19 | 0 | 1 | 55 | 124 | 178 | 4.68e-23 | 85.9 |
| MS.gene80967.t1 | AT5G38410 | 65.455 | 55 | 19 | 0 | 1 | 55 | 117 | 171 | 4.75e-23 | 85.5 |
Find 11 sgRNAs with CRISPR-Local
Find 10 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGTGGAAGTTGCCTTTGTT+TGG | 0.411503 | 7.3:-90808212 | MS.gene80967:CDS |
| CATCAAGCTCCTTCAACACT+TGG | 0.411643 | 7.3:+90808174 | None:intergenic |
| TGATGCTTCCCAAGTGTTGA+AGG | 0.425106 | 7.3:-90808183 | MS.gene80967:CDS |
| ATGATACGGACAAAGGCATC+AGG | 0.443113 | 7.3:+90808134 | None:intergenic |
| TCTCAAATTTAGTAGACTGC+AGG | 0.515110 | 7.3:+90808059 | None:intergenic |
| GATGCCTTTGTCCGTATCAT+CGG | 0.516870 | 7.3:-90808131 | MS.gene80967:CDS |
| ATCAAGCTCCTTCAACACTT+GGG | 0.552649 | 7.3:+90808175 | None:intergenic |
| CGTTGTCGTATCCGATGATA+CGG | 0.556365 | 7.3:+90808120 | None:intergenic |
| GCATCAGTTGCTCCAAACAA+AGG | 0.598394 | 7.3:+90808200 | None:intergenic |
| TGATACGGACAAAGGCATCA+GGG | 0.651232 | 7.3:+90808135 | None:intergenic |
| GTATCCGATGATACGGACAA+AGG | 0.728734 | 7.3:+90808127 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATCAAGCTCCTTCAACACTT+GGG | + | chr7.3:90808107-90808126 | None:intergenic | 40.0% | |
| ATGTGGAAGTTGCCTTTGTT+TGG | - | chr7.3:90808067-90808086 | MS.gene80967:CDS | 40.0% | |
| CATCAAGCTCCTTCAACACT+TGG | + | chr7.3:90808108-90808127 | None:intergenic | 45.0% | |
| CGTTGTCGTATCCGATGATA+CGG | + | chr7.3:90808162-90808181 | None:intergenic | 45.0% | |
| GATGCCTTTGTCCGTATCAT+CGG | - | chr7.3:90808148-90808167 | MS.gene80967:CDS | 45.0% | |
| GCATCAGTTGCTCCAAACAA+AGG | + | chr7.3:90808082-90808101 | None:intergenic | 45.0% | |
| GTATCCGATGATACGGACAA+AGG | + | chr7.3:90808155-90808174 | None:intergenic | 45.0% | |
| !! | ATGATACGGACAAAGGCATC+AGG | + | chr7.3:90808148-90808167 | None:intergenic | 45.0% |
| !! | TGATACGGACAAAGGCATCA+GGG | + | chr7.3:90808147-90808166 | None:intergenic | 45.0% |
| !! | TGATGCTTCCCAAGTGTTGA+AGG | - | chr7.3:90808096-90808115 | MS.gene80967:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 90808067 | 90808234 | 90808067 | ID=MS.gene80967 |
| chr7.3 | mRNA | 90808067 | 90808234 | 90808067 | ID=MS.gene80967.t1;Parent=MS.gene80967 |
| chr7.3 | exon | 90808067 | 90808234 | 90808067 | ID=MS.gene80967.t1.exon1;Parent=MS.gene80967.t1 |
| chr7.3 | CDS | 90808067 | 90808234 | 90808067 | ID=cds.MS.gene80967.t1;Parent=MS.gene80967.t1 |
| Gene Sequence |
| Protein sequence |
