Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81019.t1 | RHN74301.1 | 97.6 | 83 | 2 | 0 | 1 | 83 | 107 | 189 | 4.30E-38 | 166.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81019.t1 | O82497 | 56.4 | 78 | 34 | 0 | 1 | 78 | 290 | 367 | 1.1e-16 | 87.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81019.t1 | A0A072V950 | 97.6 | 83 | 2 | 0 | 1 | 83 | 293 | 375 | 3.1e-38 | 166.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053008 | MS.gene81019 | 0.802282 | 6.04E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81019.t1 | MTR_2g061470 | 97.590 | 83 | 2 | 0 | 1 | 83 | 293 | 375 | 3.84e-54 | 172 |
| MS.gene81019.t1 | MTR_7g091320 | 40.278 | 72 | 42 | 1 | 8 | 79 | 298 | 368 | 2.61e-13 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81019.t1 | AT4G10650 | 56.410 | 78 | 34 | 0 | 1 | 78 | 290 | 367 | 3.89e-22 | 88.2 |
| MS.gene81019.t1 | AT2G41670 | 44.872 | 78 | 42 | 1 | 2 | 79 | 179 | 255 | 3.84e-17 | 73.6 |
| MS.gene81019.t1 | AT2G41670 | 44.872 | 78 | 42 | 1 | 2 | 79 | 295 | 371 | 5.21e-17 | 73.9 |
Find 14 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATGGTCAGTGAATATTTC+TGG | 0.186267 | 2.1:-35579952 | None:intergenic |
| GAAACGATTTCATCCTTTGA+TGG | 0.307728 | 2.1:+35579753 | MS.gene81019:CDS |
| GATGCTCATGTCGTGGTTGC+CGG | 0.365316 | 2.1:+35579870 | MS.gene81019:CDS |
| TGGTCAGTTTACCGCGTTGC+AGG | 0.378740 | 2.1:+35579815 | MS.gene81019:CDS |
| TATAAGATGTGAAGTTGAAA+TGG | 0.388982 | 2.1:+35579779 | MS.gene81019:CDS |
| GAAATGGAAGCACTTATTGC+TGG | 0.449784 | 2.1:+35579795 | MS.gene81019:CDS |
| ATGCTCATGTCGTGGTTGCC+GGG | 0.464219 | 2.1:+35579871 | MS.gene81019:CDS |
| AATGGTCAGTGAATATTTCT+GGG | 0.476571 | 2.1:-35579951 | None:intergenic |
| ACATCTTATATTACCATCAA+AGG | 0.508833 | 2.1:-35579766 | None:intergenic |
| GTCAGTGAATATTTCTGGGA+AGG | 0.545922 | 2.1:-35579947 | None:intergenic |
| TTTGCAGGACGACATGGTGC+AGG | 0.557475 | 2.1:+35579710 | None:intergenic |
| CTTCACGTTCAGTTGAAACG+TGG | 0.582669 | 2.1:-35579845 | None:intergenic |
| GTGGAAAACTTCCTGCAACG+CGG | 0.694980 | 2.1:-35579826 | None:intergenic |
| TGAAGAAGATGCTCATGTCG+TGG | 0.731208 | 2.1:+35579863 | MS.gene81019:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAAAGATTAAGCAATTTCC+CGG | - | chr2.1:35579892-35579911 | None:intergenic | 25.0% |
| ! | ACATCTTATATTACCATCAA+AGG | - | chr2.1:35579769-35579788 | None:intergenic | 25.0% |
| ! | TATAAGATGTGAAGTTGAAA+TGG | + | chr2.1:35579779-35579798 | MS.gene81019:CDS | 25.0% |
| !! | TTGCTTAATCTTTTTCGTAC+AGG | + | chr2.1:35579897-35579916 | MS.gene81019:CDS | 30.0% |
| GAAACGATTTCATCCTTTGA+TGG | + | chr2.1:35579753-35579772 | MS.gene81019:CDS | 35.0% | |
| !! | GAAATGGAAGCACTTATTGC+TGG | + | chr2.1:35579795-35579814 | MS.gene81019:CDS | 40.0% |
| CTTCACGTTCAGTTGAAACG+TGG | - | chr2.1:35579848-35579867 | None:intergenic | 45.0% | |
| TGAAGAAGATGCTCATGTCG+TGG | + | chr2.1:35579863-35579882 | MS.gene81019:CDS | 45.0% | |
| !! | CTTTTTCGTACAGGTCGTCT+TGG | + | chr2.1:35579906-35579925 | MS.gene81019:CDS | 45.0% |
| GTGGAAAACTTCCTGCAACG+CGG | - | chr2.1:35579829-35579848 | None:intergenic | 50.0% | |
| ATGCTCATGTCGTGGTTGCC+GGG | + | chr2.1:35579871-35579890 | MS.gene81019:CDS | 55.0% | |
| GATGCTCATGTCGTGGTTGC+CGG | + | chr2.1:35579870-35579889 | MS.gene81019:CDS | 55.0% | |
| TGGTCAGTTTACCGCGTTGC+AGG | + | chr2.1:35579815-35579834 | MS.gene81019:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 35579717 | 35579968 | 35579717 | ID=MS.gene81019 |
| chr2.1 | mRNA | 35579717 | 35579968 | 35579717 | ID=MS.gene81019.t1;Parent=MS.gene81019 |
| chr2.1 | exon | 35579717 | 35579968 | 35579717 | ID=MS.gene81019.t1.exon1;Parent=MS.gene81019.t1 |
| chr2.1 | CDS | 35579717 | 35579968 | 35579717 | ID=cds.MS.gene81019.t1;Parent=MS.gene81019.t1 |
| Gene Sequence |
| Protein sequence |
