Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81038.t1 | XP_003627446.1 | 91.8 | 134 | 10 | 1 | 1 | 134 | 1 | 133 | 1.20E-53 | 219.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81038.t1 | B7FMF3 | 91.8 | 134 | 10 | 1 | 1 | 134 | 1 | 133 | 8.6e-54 | 219.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051026 | MS.gene81038 | 0.814699 | 1.35E-51 | -1.69E-46 |
| MS.gene058974 | MS.gene81038 | 0.840298 | 9.46E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81038.t1 | MTR_8g023100 | 94.030 | 134 | 7 | 1 | 1 | 134 | 1 | 133 | 1.52e-91 | 261 |
| MS.gene81038.t1 | MTR_7g117550 | 53.488 | 129 | 45 | 6 | 1 | 123 | 1 | 120 | 2.10e-35 | 120 |
| MS.gene81038.t1 | MTR_7g117550 | 55.670 | 97 | 39 | 3 | 1 | 97 | 23 | 115 | 1.65e-29 | 103 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81038.t1 | AT3G14280 | 46.250 | 80 | 36 | 2 | 49 | 128 | 48 | 120 | 6.14e-15 | 67.0 |
Find 20 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATTATCATATTTCTGAAT+TGG | 0.275390 | 8.4:-71619104 | None:intergenic |
| AATTATCATATTTCTGAATT+GGG | 0.345181 | 8.4:-71619103 | None:intergenic |
| AGTTGGTTTCAAATTGCAAA+AGG | 0.405294 | 8.4:+71620018 | MS.gene81038:CDS |
| CTTTGGAGCATAGCATCGTT+TGG | 0.411384 | 8.4:-71619159 | None:intergenic |
| TGAATCTTCTAAAACAAAGT+TGG | 0.441522 | 8.4:+71620001 | MS.gene81038:CDS |
| CTTGAAATTTGTCTATAAGA+TGG | 0.456782 | 8.4:-71619679 | None:intergenic |
| GTTGGTTTCAAATTGCAAAA+GGG | 0.500062 | 8.4:+71620019 | MS.gene81038:CDS |
| ATTTGTGAAGGGTATGATGA+AGG | 0.501766 | 8.4:+71619655 | MS.gene81038:CDS |
| GACCAACGGTTCATCACAAA+AGG | 0.504204 | 8.4:+71619701 | MS.gene81038:CDS |
| GAAATTGATGATGCAATTGA+AGG | 0.528412 | 8.4:+71620045 | MS.gene81038:CDS |
| GTGGAGAAATAATTTGTGAA+GGG | 0.550320 | 8.4:+71619644 | MS.gene81038:CDS |
| AGTGGAGAAATAATTTGTGA+AGG | 0.561503 | 8.4:+71619643 | MS.gene81038:CDS |
| TTTGTGAAGGGTATGATGAA+GGG | 0.566441 | 8.4:+71619656 | MS.gene81038:CDS |
| CACAGCATCCATCAAGAACA+AGG | 0.570428 | 8.4:+71619217 | MS.gene81038:CDS |
| TAGACAAATTTCAAGACCAA+CGG | 0.578411 | 8.4:+71619687 | MS.gene81038:CDS |
| TGAAGGTGGTCGTTTGCAAG+AGG | 0.580771 | 8.4:+71620062 | MS.gene81038:CDS |
| TTATTTCTCCACTCTCATCT+CGG | 0.581409 | 8.4:-71619633 | None:intergenic |
| AAAGTCAACAGTTGGTCCAG+TGG | 0.602548 | 8.4:+71619184 | MS.gene81038:CDS |
| ATTTGCTACCGAGATGAGAG+TGG | 0.626981 | 8.4:+71619625 | MS.gene81038:CDS |
| ATTGATGATGCAATTGAAGG+TGG | 0.706142 | 8.4:+71620048 | MS.gene81038:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAGATGAAAAAAAACTT+TGG | + | chr8.4:71619339-71619358 | MS.gene81038:intron | 15.0% |
| !! | AAGAATAGATAAAAGATAAT+AGG | - | chr8.4:71619250-71619269 | None:intergenic | 15.0% |
| !! | ATAGATATATGTATATCAAT+TGG | - | chr8.4:71619293-71619312 | None:intergenic | 15.0% |
| !! | TAGATAATATTCAATCAAAA+AGG | + | chr8.4:71619724-71619743 | MS.gene81038:intron | 15.0% |
| !!! | AAAATGACAATTTTATTCTT+CGG | + | chr8.4:71619786-71619805 | MS.gene81038:intron | 15.0% |
| !!! | AATTATCATATTTCTGAATT+GGG | - | chr8.4:71619106-71619125 | None:intergenic | 15.0% |
| !!! | AGAATAAAATTGTCATTTTA+TGG | - | chr8.4:71619785-71619804 | None:intergenic | 15.0% |
| !!! | AGATAAGTTTTTTCTTTTTT+TGG | + | chr8.4:71619459-71619478 | MS.gene81038:intron | 15.0% |
| !!! | TACCTTATAGTTTTTTTTAA+GGG | - | chr8.4:71619427-71619446 | None:intergenic | 15.0% |
| !! | AAATACCAAATTAACATCAT+CGG | - | chr8.4:71619853-71619872 | None:intergenic | 20.0% |
| !! | AATTGATGTTTATATTCTGA+AGG | + | chr8.4:71619749-71619768 | MS.gene81038:intron | 20.0% |
| !! | ATATAAATTTGTCTTACCAA+TGG | + | chr8.4:71619945-71619964 | MS.gene81038:intron | 20.0% |
| !!! | AAAAAAACTTTGGTTAGTTA+TGG | + | chr8.4:71619349-71619368 | MS.gene81038:intron | 20.0% |
| !!! | GAATTATCATATTTCTGAAT+TGG | - | chr8.4:71619107-71619126 | None:intergenic | 20.0% |
| !!! | GTACCTTATAGTTTTTTTTA+AGG | - | chr8.4:71619428-71619447 | None:intergenic | 20.0% |
| !!! | TGGATATATCTACTTTTTAT+TGG | + | chr8.4:71619479-71619498 | MS.gene81038:intron | 20.0% |
| ! | CTTGAAATTTGTCTATAAGA+TGG | - | chr8.4:71619682-71619701 | None:intergenic | 25.0% |
| ! | GTCCCTTAAAAAAAACTATA+AGG | + | chr8.4:71619422-71619441 | MS.gene81038:intron | 25.0% |
| ! | TGTCTAATTTGATACTAAAC+AGG | + | chr8.4:71619573-71619592 | MS.gene81038:intron | 25.0% |
| ! | TTTCTCCTAGTAGTAAAAAT+AGG | + | chr8.4:71619515-71619534 | MS.gene81038:intron | 25.0% |
| ! | TTTGTCTCTAGTTATATCTT+TGG | + | chr8.4:71619396-71619415 | MS.gene81038:intron | 25.0% |
| !! | TTTGGTTAGTTATGGAAATT+TGG | + | chr8.4:71619357-71619376 | MS.gene81038:intron | 25.0% |
| !!! | ATGTTAATTTGGTATTTTCG+TGG | + | chr8.4:71619856-71619875 | MS.gene81038:intron | 25.0% |
| !!! | TACTAAACAGGTTTTTTATG+AGG | + | chr8.4:71619585-71619604 | MS.gene81038:intron | 25.0% |
| !!! | TGAATCTTCTAAAACAAAGT+TGG | + | chr8.4:71620001-71620020 | MS.gene81038:CDS | 25.0% |
| ACTATAAGGTACGTCAAAAT+TGG | + | chr8.4:71619436-71619455 | MS.gene81038:intron | 30.0% | |
| AGCAGAACAAAAAATTCCAT+TGG | - | chr8.4:71619964-71619983 | None:intergenic | 30.0% | |
| TAGACAAATTTCAAGACCAA+CGG | + | chr8.4:71619687-71619706 | MS.gene81038:CDS | 30.0% | |
| TGCTACCAGTTAAAATCTTT+CGG | + | chr8.4:71619817-71619836 | MS.gene81038:intron | 30.0% | |
| ! | AGTGGAGAAATAATTTGTGA+AGG | + | chr8.4:71619643-71619662 | MS.gene81038:CDS | 30.0% |
| ! | AGTTGGTTTCAAATTGCAAA+AGG | + | chr8.4:71620018-71620037 | MS.gene81038:CDS | 30.0% |
| ! | ATGACAATTTTATTCTTCGG+CGG | + | chr8.4:71619789-71619808 | MS.gene81038:intron | 30.0% |
| ! | GTGGAGAAATAATTTGTGAA+GGG | + | chr8.4:71619644-71619663 | MS.gene81038:CDS | 30.0% |
| ! | GTTGGTTTCAAATTGCAAAA+GGG | + | chr8.4:71620019-71620038 | MS.gene81038:CDS | 30.0% |
| !! | GAAATTGATGATGCAATTGA+AGG | + | chr8.4:71620045-71620064 | MS.gene81038:CDS | 30.0% |
| !! | GATTTTAACCTTGTTCTTGA+TGG | - | chr8.4:71619228-71619247 | None:intergenic | 30.0% |
| !!! | GGAATTTTTTGTTCTGCTTA+GGG | + | chr8.4:71619966-71619985 | MS.gene81038:intron | 30.0% |
| !!! | TGGAATTTTTTGTTCTGCTT+AGG | + | chr8.4:71619965-71619984 | MS.gene81038:intron | 30.0% |
| CCAAAGAAAAAGTCAACAGT+TGG | + | chr8.4:71619176-71619195 | MS.gene81038:CDS | 35.0% | |
| CCTAGTAGTAAAAATAGGCT+AGG | + | chr8.4:71619520-71619539 | MS.gene81038:intron | 35.0% | |
| TACTGCCGATGATGTTAATT+TGG | + | chr8.4:71619845-71619864 | MS.gene81038:intron | 35.0% | |
| TTCCTAGCATTCTTTGATGT+CGG | + | chr8.4:71619882-71619901 | MS.gene81038:intron | 35.0% | |
| ! | ATTTGTGAAGGGTATGATGA+AGG | + | chr8.4:71619655-71619674 | MS.gene81038:CDS | 35.0% |
| ! | TTATTTCTCCACTCTCATCT+CGG | - | chr8.4:71619636-71619655 | None:intergenic | 35.0% |
| ! | TTTGTGAAGGGTATGATGAA+GGG | + | chr8.4:71619656-71619675 | MS.gene81038:CDS | 35.0% |
| ! | TTTTATGAGGATGAGTCTCA+AGG | + | chr8.4:71619598-71619617 | MS.gene81038:CDS | 35.0% |
| !! | AACTGCCGAAAGATTTTAAC+TGG | - | chr8.4:71619825-71619844 | None:intergenic | 35.0% |
| !! | ATTGATGATGCAATTGAAGG+TGG | + | chr8.4:71620048-71620067 | MS.gene81038:CDS | 35.0% |
| !!! | CCAACTGTTGACTTTTTCTT+TGG | - | chr8.4:71619179-71619198 | None:intergenic | 35.0% |
| !!! | CCTAGCCTATTTTTACTACT+AGG | - | chr8.4:71619523-71619542 | None:intergenic | 35.0% |
| ATAGGCTAGGACTGAGATTA+AGG | + | chr8.4:71619533-71619552 | MS.gene81038:intron | 40.0% | |
| TTCCGACATCAAAGAATGCT+AGG | - | chr8.4:71619887-71619906 | None:intergenic | 40.0% | |
| ! | GCTGTGATTTTCAATACCAC+TGG | - | chr8.4:71619203-71619222 | None:intergenic | 40.0% |
| ! | TACCTTTTGTGATGAACCGT+TGG | - | chr8.4:71619706-71619725 | None:intergenic | 40.0% |
| AAAGTCAACAGTTGGTCCAG+TGG | + | chr8.4:71619184-71619203 | MS.gene81038:CDS | 45.0% | |
| CACAGCATCCATCAAGAACA+AGG | + | chr8.4:71619217-71619236 | MS.gene81038:CDS | 45.0% | |
| GACCAACGGTTCATCACAAA+AGG | + | chr8.4:71619701-71619720 | MS.gene81038:CDS | 45.0% | |
| ! | ATTTGCTACCGAGATGAGAG+TGG | + | chr8.4:71619625-71619644 | MS.gene81038:CDS | 45.0% |
| !! | CTTTGGAGCATAGCATCGTT+TGG | - | chr8.4:71619162-71619181 | None:intergenic | 45.0% |
| TGAAGGTGGTCGTTTGCAAG+AGG | + | chr8.4:71620062-71620081 | MS.gene81038:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 71619071 | 71620095 | 71619071 | ID=MS.gene81038 |
| chr8.4 | mRNA | 71619071 | 71620095 | 71619071 | ID=MS.gene81038.t1;Parent=MS.gene81038 |
| chr8.4 | exon | 71619071 | 71619238 | 71619071 | ID=MS.gene81038.t1.exon1;Parent=MS.gene81038.t1 |
| chr8.4 | CDS | 71619071 | 71619238 | 71619071 | ID=cds.MS.gene81038.t1;Parent=MS.gene81038.t1 |
| chr8.4 | exon | 71619595 | 71619722 | 71619595 | ID=MS.gene81038.t1.exon2;Parent=MS.gene81038.t1 |
| chr8.4 | CDS | 71619595 | 71619722 | 71619595 | ID=cds.MS.gene81038.t1;Parent=MS.gene81038.t1 |
| chr8.4 | exon | 71619987 | 71620095 | 71619987 | ID=MS.gene81038.t1.exon3;Parent=MS.gene81038.t1 |
| chr8.4 | CDS | 71619987 | 71620095 | 71619987 | ID=cds.MS.gene81038.t1;Parent=MS.gene81038.t1 |
| Gene Sequence |
| Protein sequence |
