Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051026.t1 | XP_003627446.1 | 94.8 | 134 | 6 | 1 | 1 | 134 | 1 | 133 | 2.40E-54 | 221.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051026.t1 | B7FMF3 | 94.8 | 134 | 6 | 1 | 1 | 134 | 1 | 133 | 1.7e-54 | 221.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051026 | MS.gene059819 | 0.811841 | 5.72E-51 | -1.69E-46 |
| MS.gene051026 | MS.gene39903 | 0.803812 | 2.92E-49 | -1.69E-46 |
| MS.gene051026 | MS.gene49339 | 0.804809 | 1.81E-49 | -1.69E-46 |
| MS.gene051026 | MS.gene50585 | 0.801017 | 1.10E-48 | -1.69E-46 |
| MS.gene051026 | MS.gene66360 | 0.804675 | 1.93E-49 | -1.69E-46 |
| MS.gene051026 | MS.gene68181 | 0.810612 | 1.06E-50 | -1.69E-46 |
| MS.gene051026 | MS.gene70806 | -0.820214 | 7.72E-53 | -1.69E-46 |
| MS.gene051026 | MS.gene76224 | 0.831837 | 1.33E-55 | -1.69E-46 |
| MS.gene051026 | MS.gene81038 | 0.814699 | 1.35E-51 | -1.69E-46 |
| MS.gene051026 | MS.gene92272 | 0.922407 | 1.02E-88 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051026.t1 | MTR_8g023100 | 95.522 | 134 | 5 | 1 | 1 | 134 | 1 | 133 | 3.60e-93 | 265 |
| MS.gene051026.t1 | MTR_7g117550 | 54.264 | 129 | 44 | 6 | 1 | 123 | 1 | 120 | 7.95e-37 | 124 |
| MS.gene051026.t1 | MTR_7g117550 | 56.701 | 97 | 38 | 3 | 1 | 97 | 23 | 115 | 8.09e-31 | 107 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051026.t1 | AT3G14280 | 39.815 | 108 | 57 | 3 | 21 | 128 | 21 | 120 | 4.60e-15 | 67.4 |
Find 20 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATTATCATATTTCTGAAT+TGG | 0.275390 | 8.3:-68896985 | None:intergenic |
| AATTATCATATTTCTGAATT+GGG | 0.345181 | 8.3:-68896984 | None:intergenic |
| AGTTGGTTTCAAATTGCAAA+AGG | 0.405294 | 8.3:+68897898 | MS.gene051026:CDS |
| CTTTGGAGCATAGCATCGTT+TGG | 0.411384 | 8.3:-68897040 | None:intergenic |
| TGAATCTTCTAAAACAAAGT+TGG | 0.441522 | 8.3:+68897881 | MS.gene051026:CDS |
| CTTGAAATTTGTCTATAAGA+TGG | 0.456782 | 8.3:-68897560 | None:intergenic |
| TTATTTCTCCACTCTCATCT+TGG | 0.480024 | 8.3:-68897514 | None:intergenic |
| GTTGGTTTCAAATTGCAAAA+GGG | 0.500062 | 8.3:+68897899 | MS.gene051026:CDS |
| ATTTGTGAAGGGTATGATGA+AGG | 0.501766 | 8.3:+68897536 | MS.gene051026:CDS |
| GACCAACGGTTCATCACAAA+AGG | 0.504204 | 8.3:+68897582 | MS.gene051026:CDS |
| GAAATTGATGATGCAATTGA+AGG | 0.528412 | 8.3:+68897925 | MS.gene051026:CDS |
| GTGGAGAAATAATTTGTGAA+GGG | 0.550320 | 8.3:+68897525 | MS.gene051026:CDS |
| AGTGGAGAAATAATTTGTGA+AGG | 0.561503 | 8.3:+68897524 | MS.gene051026:CDS |
| TTTGTGAAGGGTATGATGAA+GGG | 0.566441 | 8.3:+68897537 | MS.gene051026:CDS |
| CACAGCATCCATCAAGAACA+AGG | 0.570428 | 8.3:+68897098 | MS.gene051026:CDS |
| TAGACAAATTTCAAGACCAA+CGG | 0.578411 | 8.3:+68897568 | MS.gene051026:CDS |
| TGAAGGTGGTCGTTTGCAAG+AGG | 0.580771 | 8.3:+68897942 | MS.gene051026:CDS |
| AAAGTCAACAGTTGGTCCAG+TGG | 0.602548 | 8.3:+68897065 | MS.gene051026:CDS |
| ATTTGCTACCAAGATGAGAG+TGG | 0.633835 | 8.3:+68897506 | MS.gene051026:CDS |
| ATTGATGATGCAATTGAAGG+TGG | 0.706142 | 8.3:+68897928 | MS.gene051026:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAGATGAAAAAAACTT+TGG | + | chr8.3:68897220-68897239 | MS.gene051026:intron | 15.0% |
| !! | AAGAATAGATAAAAGATAAT+AGG | - | chr8.3:68897131-68897150 | None:intergenic | 15.0% |
| !! | ATAGATATATGTATATCAAT+TGG | - | chr8.3:68897174-68897193 | None:intergenic | 15.0% |
| !!! | AAAATGACAATTTTATTCTT+CGG | + | chr8.3:68897667-68897686 | MS.gene051026:intron | 15.0% |
| !!! | AATTATCATATTTCTGAATT+GGG | - | chr8.3:68896987-68897006 | None:intergenic | 15.0% |
| !!! | AGAATAAAATTGTCATTTTA+TGG | - | chr8.3:68897666-68897685 | None:intergenic | 15.0% |
| !! | AATTGATGTTTATATTCTGA+AGG | + | chr8.3:68897630-68897649 | MS.gene051026:intron | 20.0% |
| !! | ATATAAATATGTCTTACCAA+TGG | + | chr8.3:68897825-68897844 | MS.gene051026:intron | 20.0% |
| !!! | AAAAAAACTTTGGTTAGTTA+TGG | + | chr8.3:68897230-68897249 | MS.gene051026:intron | 20.0% |
| !!! | GAATTATCATATTTCTGAAT+TGG | - | chr8.3:68896988-68897007 | None:intergenic | 20.0% |
| !!! | GGATAAGTTTTTTCTTTTTT+TGG | + | chr8.3:68897340-68897359 | MS.gene051026:intron | 20.0% |
| !!! | TACCTTATAGTTTTTTCTAA+GGG | - | chr8.3:68897308-68897327 | None:intergenic | 20.0% |
| !!! | TGGATATATCTACTTTTTAT+TGG | + | chr8.3:68897360-68897379 | MS.gene051026:intron | 20.0% |
| ! | CTTGAAATTTGTCTATAAGA+TGG | - | chr8.3:68897563-68897582 | None:intergenic | 25.0% |
| ! | TGTCTAATTTGATACTAAAC+AGG | + | chr8.3:68897454-68897473 | MS.gene051026:intron | 25.0% |
| ! | TTTCTCCTAGTAGTAAAAAT+AGG | + | chr8.3:68897396-68897415 | MS.gene051026:intron | 25.0% |
| ! | TTTGTCTCTAGTTATATCTT+TGG | + | chr8.3:68897277-68897296 | MS.gene051026:intron | 25.0% |
| !! | TTTGGTTAGTTATGGAAATT+TGG | + | chr8.3:68897238-68897257 | MS.gene051026:intron | 25.0% |
| !!! | GTACCTTATAGTTTTTTCTA+AGG | - | chr8.3:68897309-68897328 | None:intergenic | 25.0% |
| !!! | TACTAAACAGGTTTTTTATG+AGG | + | chr8.3:68897466-68897485 | MS.gene051026:intron | 25.0% |
| !!! | TGAATCTTCTAAAACAAAGT+TGG | + | chr8.3:68897881-68897900 | MS.gene051026:CDS | 25.0% |
| AAATGTCGATGAGGTTAATT+TGG | + | chr8.3:68897726-68897745 | MS.gene051026:intron | 30.0% | |
| ACTATAAGGTACGTCAAAAT+TGG | + | chr8.3:68897317-68897336 | MS.gene051026:intron | 30.0% | |
| AGCACAACAAAAAATTCCAT+TGG | - | chr8.3:68897844-68897863 | None:intergenic | 30.0% | |
| CTATAAGGTACGTCAAAATT+GGG | + | chr8.3:68897318-68897337 | MS.gene051026:intron | 30.0% | |
| GTCCCTTAGAAAAAACTATA+AGG | + | chr8.3:68897303-68897322 | MS.gene051026:intron | 30.0% | |
| TAGACAAATTTCAAGACCAA+CGG | + | chr8.3:68897568-68897587 | MS.gene051026:CDS | 30.0% | |
| TATAAGGTACGTCAAAATTG+GGG | + | chr8.3:68897319-68897338 | MS.gene051026:intron | 30.0% | |
| TGCTACCAGTTAAAATCTTT+CGG | + | chr8.3:68897698-68897717 | MS.gene051026:intron | 30.0% | |
| ! | AGTGGAGAAATAATTTGTGA+AGG | + | chr8.3:68897524-68897543 | MS.gene051026:CDS | 30.0% |
| ! | AGTTGGTTTCAAATTGCAAA+AGG | + | chr8.3:68897898-68897917 | MS.gene051026:CDS | 30.0% |
| ! | ATGACAATTTTATTCTTCGG+CGG | + | chr8.3:68897670-68897689 | MS.gene051026:intron | 30.0% |
| ! | GTGGAGAAATAATTTGTGAA+GGG | + | chr8.3:68897525-68897544 | MS.gene051026:CDS | 30.0% |
| ! | GTTGGTTTCAAATTGCAAAA+GGG | + | chr8.3:68897899-68897918 | MS.gene051026:CDS | 30.0% |
| ! | TTTCTAGCATTCTTTGATGT+CGG | + | chr8.3:68897763-68897782 | MS.gene051026:intron | 30.0% |
| !! | AGGTTAATTTGGTATTTTCG+TGG | + | chr8.3:68897737-68897756 | MS.gene051026:intron | 30.0% |
| !! | GAAATTGATGATGCAATTGA+AGG | + | chr8.3:68897925-68897944 | MS.gene051026:CDS | 30.0% |
| !! | GATTTTAACCTTGTTCTTGA+TGG | - | chr8.3:68897109-68897128 | None:intergenic | 30.0% |
| !!! | GGAATTTTTTGTTGTGCTTA+GGG | + | chr8.3:68897846-68897865 | MS.gene051026:intron | 30.0% |
| !!! | TGGAATTTTTTGTTGTGCTT+AGG | + | chr8.3:68897845-68897864 | MS.gene051026:intron | 30.0% |
| CCAAAGAAAAAGTCAACAGT+TGG | + | chr8.3:68897057-68897076 | MS.gene051026:CDS | 35.0% | |
| CCTAGTAGTAAAAATAGGCT+AGG | + | chr8.3:68897401-68897420 | MS.gene051026:intron | 35.0% | |
| TACCAGTTAAAATCTTTCGG+CGG | + | chr8.3:68897701-68897720 | MS.gene051026:intron | 35.0% | |
| ! | ATTTGTGAAGGGTATGATGA+AGG | + | chr8.3:68897536-68897555 | MS.gene051026:CDS | 35.0% |
| ! | TTATTTCTCCACTCTCATCT+TGG | - | chr8.3:68897517-68897536 | None:intergenic | 35.0% |
| ! | TTTGTGAAGGGTATGATGAA+GGG | + | chr8.3:68897537-68897556 | MS.gene051026:CDS | 35.0% |
| ! | TTTTATGAGGATGAGTCTCA+AGG | + | chr8.3:68897479-68897498 | MS.gene051026:CDS | 35.0% |
| !! | ATTGATGATGCAATTGAAGG+TGG | + | chr8.3:68897928-68897947 | MS.gene051026:CDS | 35.0% |
| !!! | CCAACTGTTGACTTTTTCTT+TGG | - | chr8.3:68897060-68897079 | None:intergenic | 35.0% |
| !!! | CCTAGCCTATTTTTACTACT+AGG | - | chr8.3:68897404-68897423 | None:intergenic | 35.0% |
| ! | ATTTGCTACCAAGATGAGAG+TGG | + | chr8.3:68897506-68897525 | MS.gene051026:CDS | 40.0% |
| ! | GCTGTGATTTTCAATACCAC+TGG | - | chr8.3:68897084-68897103 | None:intergenic | 40.0% |
| ! | TACCTTTTGTGATGAACCGT+TGG | - | chr8.3:68897587-68897606 | None:intergenic | 40.0% |
| !! | AACCGCCGAAAGATTTTAAC+TGG | - | chr8.3:68897706-68897725 | None:intergenic | 40.0% |
| AAAGTCAACAGTTGGTCCAG+TGG | + | chr8.3:68897065-68897084 | MS.gene051026:CDS | 45.0% | |
| CACAGCATCCATCAAGAACA+AGG | + | chr8.3:68897098-68897117 | MS.gene051026:CDS | 45.0% | |
| GACCAACGGTTCATCACAAA+AGG | + | chr8.3:68897582-68897601 | MS.gene051026:CDS | 45.0% | |
| !! | CTTTGGAGCATAGCATCGTT+TGG | - | chr8.3:68897043-68897062 | None:intergenic | 45.0% |
| TCGGCGGTTAAATGTCGATG+AGG | + | chr8.3:68897717-68897736 | MS.gene051026:intron | 50.0% | |
| TGAAGGTGGTCGTTTGCAAG+AGG | + | chr8.3:68897942-68897961 | MS.gene051026:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 68896952 | 68897975 | 68896952 | ID=MS.gene051026 |
| chr8.3 | mRNA | 68896952 | 68897975 | 68896952 | ID=MS.gene051026.t1;Parent=MS.gene051026 |
| chr8.3 | exon | 68896952 | 68897119 | 68896952 | ID=MS.gene051026.t1.exon1;Parent=MS.gene051026.t1 |
| chr8.3 | CDS | 68896952 | 68897119 | 68896952 | ID=cds.MS.gene051026.t1;Parent=MS.gene051026.t1 |
| chr8.3 | exon | 68897476 | 68897603 | 68897476 | ID=MS.gene051026.t1.exon2;Parent=MS.gene051026.t1 |
| chr8.3 | CDS | 68897476 | 68897603 | 68897476 | ID=cds.MS.gene051026.t1;Parent=MS.gene051026.t1 |
| chr8.3 | exon | 68897867 | 68897975 | 68897867 | ID=MS.gene051026.t1.exon3;Parent=MS.gene051026.t1 |
| chr8.3 | CDS | 68897867 | 68897975 | 68897867 | ID=cds.MS.gene051026.t1;Parent=MS.gene051026.t1 |
| Gene Sequence |
| Protein sequence |
