Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81054.t1 | AAA82737.1 | 98.5 | 273 | 4 | 0 | 1 | 273 | 1 | 273 | 4.40E-148 | 533.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81054.t1 | Q01806 | 95.3 | 274 | 11 | 1 | 2 | 273 | 4 | 277 | 3.0e-145 | 515.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81054.t1 | Q42411 | 98.5 | 273 | 4 | 0 | 1 | 273 | 1 | 273 | 3.2e-148 | 533.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051005 | MS.gene81054 | 0.82897 | 6.69E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81054.t1 | MTR_8g023510 | 94.981 | 259 | 11 | 1 | 1 | 257 | 3 | 261 | 0.0 | 503 |
| MS.gene81054.t1 | MTR_3g463370 | 59.786 | 281 | 65 | 5 | 1 | 273 | 1 | 241 | 1.55e-101 | 296 |
| MS.gene81054.t1 | MTR_4g023810 | 60.236 | 254 | 85 | 6 | 22 | 273 | 25 | 264 | 2.82e-100 | 294 |
| MS.gene81054.t1 | MTR_8g068040 | 38.281 | 256 | 143 | 7 | 10 | 255 | 11 | 261 | 2.70e-49 | 164 |
| MS.gene81054.t1 | MTR_5g031100 | 37.970 | 266 | 137 | 6 | 17 | 255 | 14 | 278 | 5.70e-48 | 160 |
| MS.gene81054.t1 | MTR_8g068030 | 36.923 | 260 | 145 | 7 | 10 | 255 | 11 | 265 | 1.63e-47 | 159 |
| MS.gene81054.t1 | MTR_5g031160 | 38.800 | 250 | 133 | 8 | 23 | 255 | 41 | 287 | 3.18e-45 | 154 |
| MS.gene81054.t1 | MTR_5g031120 | 37.453 | 267 | 138 | 7 | 17 | 255 | 14 | 279 | 7.94e-45 | 152 |
| MS.gene81054.t1 | MTR_3g054170 | 41.379 | 261 | 142 | 8 | 1 | 256 | 1 | 255 | 1.25e-43 | 149 |
| MS.gene81054.t1 | MTR_5g031140 | 38.333 | 240 | 132 | 7 | 31 | 255 | 48 | 286 | 4.13e-43 | 148 |
| MS.gene81054.t1 | MTR_3g054180 | 41.961 | 255 | 133 | 8 | 10 | 256 | 14 | 261 | 2.85e-42 | 145 |
| MS.gene81054.t1 | MTR_8g068050 | 37.931 | 261 | 144 | 9 | 9 | 255 | 17 | 273 | 5.04e-42 | 152 |
| MS.gene81054.t1 | MTR_3g047140 | 38.168 | 262 | 150 | 8 | 7 | 262 | 13 | 268 | 8.40e-41 | 142 |
| MS.gene81054.t1 | MTR_5g031090 | 38.683 | 243 | 129 | 10 | 31 | 255 | 35 | 275 | 2.12e-39 | 139 |
| MS.gene81054.t1 | MTR_5g031030 | 37.903 | 248 | 137 | 9 | 22 | 255 | 30 | 274 | 7.02e-39 | 137 |
| MS.gene81054.t1 | MTR_3g027280 | 39.921 | 253 | 131 | 10 | 12 | 251 | 18 | 262 | 7.25e-39 | 137 |
| MS.gene81054.t1 | MTR_8g067980 | 40.000 | 240 | 119 | 12 | 31 | 253 | 26 | 257 | 3.39e-37 | 132 |
| MS.gene81054.t1 | MTR_1g090973 | 36.900 | 271 | 155 | 11 | 6 | 268 | 11 | 273 | 6.84e-37 | 132 |
| MS.gene81054.t1 | MTR_0684s0020 | 37.313 | 268 | 153 | 9 | 10 | 268 | 15 | 276 | 1.57e-35 | 129 |
| MS.gene81054.t1 | MTR_0163s0020 | 37.313 | 268 | 153 | 9 | 10 | 268 | 16 | 277 | 1.66e-35 | 128 |
| MS.gene81054.t1 | MTR_4g093080 | 34.926 | 272 | 164 | 10 | 7 | 270 | 8 | 274 | 5.05e-35 | 133 |
| MS.gene81054.t1 | MTR_1g090943 | 38.132 | 257 | 145 | 11 | 6 | 255 | 11 | 260 | 5.96e-34 | 124 |
| MS.gene81054.t1 | MTR_1g090957 | 36.905 | 252 | 128 | 10 | 12 | 251 | 18 | 250 | 6.06e-34 | 124 |
| MS.gene81054.t1 | MTR_4g093140 | 34.733 | 262 | 150 | 9 | 6 | 257 | 16 | 266 | 6.54e-34 | 130 |
| MS.gene81054.t1 | MTR_4g093110 | 33.846 | 260 | 151 | 8 | 6 | 255 | 16 | 264 | 1.00e-33 | 129 |
| MS.gene81054.t1 | MTR_8g067940 | 33.582 | 268 | 156 | 8 | 9 | 255 | 15 | 281 | 5.71e-33 | 122 |
| MS.gene81054.t1 | MTR_3g054200 | 37.302 | 252 | 147 | 8 | 10 | 256 | 10 | 255 | 7.18e-33 | 121 |
| MS.gene81054.t1 | MTR_8g067990 | 36.842 | 247 | 132 | 13 | 31 | 260 | 26 | 265 | 1.63e-31 | 117 |
| MS.gene81054.t1 | MTR_4g093070 | 35.379 | 277 | 147 | 10 | 2 | 257 | 3 | 268 | 1.44e-30 | 120 |
| MS.gene81054.t1 | MTR_8g067930 | 32.847 | 274 | 170 | 7 | 1 | 265 | 1 | 269 | 4.75e-30 | 119 |
| MS.gene81054.t1 | MTR_8g067690 | 33.906 | 233 | 144 | 5 | 32 | 255 | 63 | 294 | 7.16e-30 | 118 |
| MS.gene81054.t1 | MTR_8g067630 | 39.720 | 214 | 118 | 8 | 32 | 236 | 33 | 244 | 2.00e-29 | 117 |
| MS.gene81054.t1 | MTR_8g067720 | 30.855 | 269 | 173 | 6 | 3 | 263 | 2 | 265 | 2.18e-29 | 117 |
| MS.gene81054.t1 | MTR_1g090930 | 36.694 | 248 | 131 | 10 | 17 | 250 | 23 | 258 | 6.75e-28 | 108 |
| MS.gene81054.t1 | MTR_1g014240 | 33.054 | 239 | 144 | 8 | 32 | 256 | 40 | 276 | 1.09e-26 | 109 |
| MS.gene81054.t1 | MTR_8g067735 | 33.333 | 234 | 147 | 6 | 32 | 256 | 46 | 279 | 1.67e-26 | 108 |
| MS.gene81054.t1 | MTR_7g115740 | 31.343 | 268 | 161 | 10 | 18 | 269 | 14 | 274 | 6.54e-26 | 107 |
| MS.gene81054.t1 | MTR_1g090937 | 33.333 | 252 | 145 | 10 | 15 | 255 | 21 | 260 | 7.15e-25 | 100 |
| MS.gene81054.t1 | MTR_4g093040 | 36.170 | 188 | 109 | 6 | 67 | 244 | 73 | 259 | 2.36e-24 | 102 |
| MS.gene81054.t1 | MTR_8g067960 | 33.036 | 224 | 132 | 9 | 44 | 255 | 24 | 241 | 3.78e-24 | 97.8 |
| MS.gene81054.t1 | MTR_7g116130 | 34.498 | 229 | 128 | 9 | 43 | 255 | 39 | 261 | 1.28e-23 | 100 |
| MS.gene81054.t1 | MTR_1g090960 | 33.840 | 263 | 120 | 12 | 6 | 256 | 11 | 231 | 3.80e-23 | 95.1 |
| MS.gene81054.t1 | MTR_4g093040 | 36.517 | 178 | 102 | 6 | 77 | 244 | 1 | 177 | 5.01e-23 | 98.6 |
| MS.gene81054.t1 | MTR_1g048360 | 33.333 | 210 | 120 | 8 | 55 | 253 | 60 | 260 | 3.30e-21 | 93.2 |
| MS.gene81054.t1 | MTR_7g062940 | 31.225 | 253 | 154 | 8 | 16 | 251 | 6 | 255 | 1.36e-20 | 91.7 |
| MS.gene81054.t1 | MTR_4g093050 | 32.308 | 260 | 144 | 12 | 15 | 251 | 12 | 262 | 7.85e-19 | 86.3 |
| MS.gene81054.t1 | MTR_7g062950 | 32.000 | 225 | 136 | 5 | 43 | 251 | 32 | 255 | 8.46e-19 | 86.3 |
| MS.gene81054.t1 | MTR_7g063030 | 30.952 | 252 | 152 | 7 | 16 | 251 | 15 | 260 | 1.11e-18 | 85.9 |
| MS.gene81054.t1 | MTR_7g063030 | 32.444 | 225 | 134 | 6 | 43 | 251 | 32 | 254 | 1.31e-18 | 85.9 |
| MS.gene81054.t1 | MTR_7g062770 | 30.859 | 256 | 156 | 6 | 16 | 251 | 6 | 260 | 8.67e-18 | 83.2 |
| MS.gene81054.t1 | MTR_1g052275 | 30.502 | 259 | 156 | 7 | 14 | 251 | 3 | 258 | 3.16e-17 | 81.6 |
| MS.gene81054.t1 | MTR_5g035910 | 30.451 | 266 | 157 | 10 | 9 | 251 | 3 | 263 | 5.42e-17 | 80.9 |
| MS.gene81054.t1 | MTR_7g062890 | 31.102 | 254 | 154 | 8 | 16 | 251 | 6 | 256 | 7.33e-17 | 80.5 |
| MS.gene81054.t1 | MTR_5g025020 | 30.233 | 215 | 124 | 7 | 66 | 262 | 85 | 291 | 1.22e-16 | 80.1 |
| MS.gene81054.t1 | MTR_4g056320 | 28.736 | 261 | 125 | 10 | 10 | 256 | 17 | 230 | 1.72e-16 | 77.4 |
| MS.gene81054.t1 | MTR_7g062700 | 31.128 | 257 | 152 | 8 | 16 | 251 | 6 | 258 | 2.74e-16 | 79.0 |
| MS.gene81054.t1 | MTR_5g077100 | 31.771 | 192 | 115 | 5 | 69 | 253 | 68 | 250 | 3.76e-16 | 78.6 |
| MS.gene81054.t1 | MTR_7g063010 | 27.843 | 255 | 166 | 6 | 13 | 251 | 4 | 256 | 4.16e-15 | 75.5 |
| MS.gene81054.t1 | MTR_7g062750 | 28.516 | 256 | 160 | 7 | 16 | 251 | 6 | 258 | 7.88e-15 | 74.7 |
| MS.gene81054.t1 | MTR_3g068025 | 30.385 | 260 | 158 | 8 | 14 | 251 | 9 | 267 | 2.77e-14 | 72.8 |
| MS.gene81054.t1 | MTR_8g068050 | 33.333 | 132 | 80 | 4 | 9 | 134 | 17 | 146 | 1.69e-13 | 70.5 |
| MS.gene81054.t1 | MTR_2g013720 | 30.112 | 269 | 154 | 11 | 5 | 251 | 6 | 262 | 3.42e-13 | 69.7 |
| MS.gene81054.t1 | MTR_7g062920 | 29.297 | 256 | 158 | 7 | 16 | 251 | 6 | 258 | 8.21e-13 | 68.6 |
| MS.gene81054.t1 | MTR_7g062660 | 30.830 | 253 | 155 | 7 | 16 | 251 | 6 | 255 | 3.60e-12 | 66.6 |
| MS.gene81054.t1 | MTR_7g062900 | 26.556 | 241 | 156 | 5 | 26 | 251 | 17 | 251 | 6.52e-12 | 65.9 |
| MS.gene81054.t1 | MTR_1g099260 | 26.016 | 246 | 151 | 9 | 29 | 253 | 14 | 249 | 1.49e-11 | 64.7 |
| MS.gene81054.t1 | MTR_7g062680 | 29.258 | 229 | 141 | 5 | 43 | 251 | 32 | 259 | 1.68e-11 | 64.7 |
| MS.gene81054.t1 | MTR_5g095970 | 29.297 | 256 | 159 | 7 | 8 | 253 | 4 | 247 | 3.20e-11 | 63.9 |
| MS.gene81054.t1 | MTR_7g062990 | 26.667 | 255 | 169 | 5 | 14 | 251 | 6 | 259 | 3.94e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81054.t1 | AT5G65600 | 33.778 | 225 | 137 | 7 | 32 | 244 | 38 | 262 | 8.84e-25 | 103 |
| MS.gene81054.t1 | AT5G10530 | 33.462 | 260 | 155 | 10 | 11 | 258 | 4 | 257 | 9.88e-25 | 103 |
| MS.gene81054.t1 | AT4G02410 | 32.510 | 243 | 147 | 6 | 25 | 251 | 22 | 263 | 1.43e-23 | 100 |
| MS.gene81054.t1 | AT2G37710 | 31.111 | 225 | 138 | 6 | 43 | 251 | 38 | 261 | 2.46e-22 | 96.7 |
| MS.gene81054.t1 | AT4G02420 | 31.321 | 265 | 157 | 9 | 6 | 251 | 2 | 260 | 2.79e-22 | 96.7 |
| MS.gene81054.t1 | AT2G29250 | 30.657 | 274 | 154 | 10 | 5 | 254 | 3 | 264 | 2.42e-21 | 94.0 |
| MS.gene81054.t1 | AT5G01540 | 30.800 | 250 | 145 | 9 | 30 | 253 | 31 | 278 | 2.49e-20 | 90.9 |
| MS.gene81054.t1 | AT3G55550 | 30.290 | 241 | 153 | 4 | 26 | 252 | 20 | 259 | 5.82e-20 | 89.7 |
| MS.gene81054.t1 | AT4G29050 | 33.186 | 226 | 123 | 8 | 48 | 251 | 39 | 258 | 5.87e-20 | 89.7 |
| MS.gene81054.t1 | AT4G29050 | 33.186 | 226 | 123 | 8 | 48 | 251 | 53 | 272 | 6.51e-20 | 89.7 |
| MS.gene81054.t1 | AT3G53810 | 30.488 | 246 | 152 | 7 | 24 | 252 | 19 | 262 | 3.16e-19 | 87.8 |
| MS.gene81054.t1 | AT5G01550 | 29.537 | 281 | 165 | 10 | 2 | 253 | 14 | 290 | 5.14e-19 | 87.0 |
| MS.gene81054.t1 | AT5G01560 | 29.482 | 251 | 142 | 10 | 32 | 253 | 30 | 274 | 1.55e-17 | 82.8 |
| MS.gene81054.t1 | AT3G15356 | 28.689 | 244 | 142 | 8 | 40 | 252 | 28 | 270 | 4.96e-17 | 79.3 |
| MS.gene81054.t1 | AT5G03140 | 30.392 | 204 | 128 | 6 | 56 | 249 | 58 | 257 | 1.14e-16 | 80.1 |
| MS.gene81054.t1 | AT5G60310 | 30.677 | 251 | 141 | 11 | 23 | 251 | 18 | 257 | 3.47e-16 | 78.6 |
| MS.gene81054.t1 | AT3G08870 | 29.960 | 247 | 146 | 10 | 32 | 253 | 57 | 301 | 6.04e-16 | 77.8 |
| MS.gene81054.t1 | AT3G08870 | 29.960 | 247 | 146 | 10 | 32 | 253 | 36 | 280 | 6.75e-16 | 77.8 |
| MS.gene81054.t1 | AT2G29220 | 29.927 | 274 | 147 | 12 | 11 | 254 | 6 | 264 | 8.35e-16 | 77.4 |
| MS.gene81054.t1 | AT5G60280 | 28.016 | 257 | 160 | 9 | 12 | 251 | 8 | 256 | 9.89e-16 | 77.4 |
| MS.gene81054.t1 | AT3G45430 | 31.679 | 262 | 137 | 13 | 26 | 260 | 22 | 268 | 2.34e-15 | 76.3 |
| MS.gene81054.t1 | AT3G45430 | 31.679 | 262 | 137 | 13 | 26 | 260 | 22 | 268 | 2.34e-15 | 76.3 |
| MS.gene81054.t1 | AT3G45430 | 31.679 | 262 | 137 | 13 | 26 | 260 | 22 | 268 | 2.34e-15 | 76.3 |
| MS.gene81054.t1 | AT3G16530 | 26.277 | 274 | 167 | 10 | 10 | 252 | 1 | 270 | 5.50e-15 | 73.6 |
| MS.gene81054.t1 | AT4G04960 | 31.687 | 243 | 143 | 9 | 25 | 249 | 16 | 253 | 1.63e-14 | 73.6 |
| MS.gene81054.t1 | AT4G04960 | 32.203 | 236 | 137 | 9 | 32 | 249 | 23 | 253 | 3.32e-14 | 72.8 |
| MS.gene81054.t1 | AT5G06740 | 31.000 | 200 | 121 | 9 | 64 | 253 | 66 | 258 | 8.18e-14 | 71.6 |
| MS.gene81054.t1 | AT5G06740 | 31.000 | 200 | 121 | 9 | 64 | 253 | 78 | 270 | 8.55e-14 | 71.6 |
| MS.gene81054.t1 | AT5G60300 | 29.918 | 244 | 139 | 12 | 30 | 251 | 25 | 258 | 1.09e-13 | 71.2 |
| MS.gene81054.t1 | AT5G60300 | 29.918 | 244 | 139 | 12 | 30 | 251 | 25 | 258 | 1.09e-13 | 71.2 |
| MS.gene81054.t1 | AT5G60300 | 29.918 | 244 | 139 | 12 | 30 | 251 | 25 | 258 | 1.31e-13 | 70.9 |
| MS.gene81054.t1 | AT1G70110 | 27.273 | 231 | 139 | 9 | 43 | 251 | 33 | 256 | 1.24e-12 | 68.2 |
| MS.gene81054.t1 | AT3G59700 | 25.641 | 234 | 155 | 7 | 34 | 249 | 17 | 249 | 1.27e-12 | 68.2 |
| MS.gene81054.t1 | AT5G03350 | 27.197 | 239 | 143 | 8 | 44 | 252 | 31 | 268 | 2.05e-12 | 66.2 |
| MS.gene81054.t1 | AT5G42120 | 29.278 | 263 | 153 | 10 | 13 | 253 | 7 | 258 | 2.31e-12 | 67.4 |
| MS.gene81054.t1 | AT3G45330 | 27.600 | 250 | 163 | 7 | 25 | 258 | 23 | 270 | 3.59e-12 | 66.6 |
| MS.gene81054.t1 | AT3G53380 | 32.000 | 250 | 150 | 10 | 10 | 249 | 5 | 244 | 1.99e-11 | 64.3 |
| MS.gene81054.t1 | AT5G60320 | 27.799 | 259 | 159 | 12 | 16 | 251 | 9 | 262 | 2.07e-11 | 64.3 |
Find 50 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTCTGCTCCTATTCATATT+TGG | 0.101617 | 8.4:+71422350 | MS.gene81054:CDS |
| CTTGGAAGATTAGATTCTTT+TGG | 0.238719 | 8.4:-71422252 | None:intergenic |
| CAGAACATGATATTCGTTAT+TGG | 0.241480 | 8.4:+71422857 | MS.gene81054:CDS |
| CCAATATGTCTATTTATCTT+TGG | 0.248398 | 8.4:-71422604 | None:intergenic |
| CTTCATCTTGTTATTCAATA+AGG | 0.258451 | 8.4:+71422180 | MS.gene81054:CDS |
| TTCTGCTCCTATTCATATTT+GGG | 0.286679 | 8.4:+71422351 | MS.gene81054:CDS |
| CATATTTGGGATAGCAAAAC+AGG | 0.355340 | 8.4:+71422364 | MS.gene81054:CDS |
| CTTCCCTGTTGAGGTAGTTA+TGG | 0.356339 | 8.4:-71422281 | None:intergenic |
| ATCGGTCGTGCAGGATTTCT+CGG | 0.366627 | 8.4:+71422502 | MS.gene81054:CDS |
| TTGCTATCCCAAATATGAAT+AGG | 0.372573 | 8.4:-71422358 | None:intergenic |
| TACCAATGTTGCAGATGGTT+TGG | 0.397603 | 8.4:+71422441 | MS.gene81054:CDS |
| CCAAAGATAAATAGACATAT+TGG | 0.401782 | 8.4:+71422604 | MS.gene81054:CDS |
| TCTGCAACATTGGTACTATA+AGG | 0.412329 | 8.4:-71422433 | None:intergenic |
| TCACTAAGGATATAACTATC+AGG | 0.422347 | 8.4:-71422760 | None:intergenic |
| TAACAAGATGAAGACTGTTA+AGG | 0.442595 | 8.4:-71422170 | None:intergenic |
| AAGGCAGTCAAGAACTCTAT+TGG | 0.450440 | 8.4:+71422316 | MS.gene81054:CDS |
| AATGCCATAACTACCTCAAC+AGG | 0.458397 | 8.4:+71422277 | MS.gene81054:CDS |
| GAGAGGCTAATGTTGTGATA+AGG | 0.465611 | 8.4:+71422686 | MS.gene81054:CDS |
| GCTAAACTTTGTGATGGTAA+AGG | 0.469149 | 8.4:-71422224 | None:intergenic |
| GGCCAAACCATCTGCAACAT+TGG | 0.479659 | 8.4:-71422443 | None:intergenic |
| CCACAAAACATCGGTCGTGC+AGG | 0.494269 | 8.4:+71422493 | MS.gene81054:CDS |
| AATAGAGTTCTTGACTGCCT+TGG | 0.497569 | 8.4:-71422314 | None:intergenic |
| ATATTGTTCCTGAGTGGGTA+AGG | 0.498309 | 8.4:+71422800 | MS.gene81054:CDS |
| AGTGTAGTTATGTCTTATCC+AGG | 0.498729 | 8.4:+71422733 | MS.gene81054:CDS |
| GGTACTATAAGGAGCAGTTA+TGG | 0.500240 | 8.4:-71422422 | None:intergenic |
| TGAAAGATATTGTTCCTGAG+TGG | 0.520340 | 8.4:+71422794 | MS.gene81054:CDS |
| GAAAGATATTGTTCCTGAGT+GGG | 0.522902 | 8.4:+71422795 | MS.gene81054:CDS |
| TAATGTTTCAAGCTCTGTGC+AGG | 0.524413 | 8.4:+71422924 | MS.gene81054:CDS |
| GTCAATAAGCACTACACCAT+GGG | 0.524982 | 8.4:+71422648 | MS.gene81054:CDS |
| ATATAACTATCAGGAAGTCC+TGG | 0.527887 | 8.4:-71422751 | None:intergenic |
| ATCGGTTTCTCAGCAGCCAC+TGG | 0.531685 | 8.4:+71422823 | MS.gene81054:CDS |
| TATAGTACCAATGTTGCAGA+TGG | 0.532177 | 8.4:+71422436 | MS.gene81054:CDS |
| TTTGGCCTTCTTCATTGCAC+CGG | 0.538662 | 8.4:+71422459 | MS.gene81054:CDS |
| GATGAAGACTGTTAAGGAGA+TGG | 0.541381 | 8.4:-71422164 | None:intergenic |
| ATCGACCGGTGCAATGAAGA+AGG | 0.544745 | 8.4:-71422464 | None:intergenic |
| GTTCCTGAGTGGGTAAGGAT+CGG | 0.550149 | 8.4:+71422805 | MS.gene81054:CDS |
| AGGTAGTTATGGCATTGCCT+TGG | 0.557576 | 8.4:-71422270 | None:intergenic |
| TTCTGCAAATTCTGCACCAG+TGG | 0.566800 | 8.4:-71422839 | None:intergenic |
| AAGTTGTAACTTCCCTGTTG+AGG | 0.568898 | 8.4:-71422290 | None:intergenic |
| CAAAAGAATCTAATCTTCCA+AGG | 0.573374 | 8.4:+71422253 | MS.gene81054:CDS |
| TCAGGAACAATATCTTTCAA+AGG | 0.574670 | 8.4:-71422790 | None:intergenic |
| AAACCGATCCTTACCCACTC+AGG | 0.577788 | 8.4:-71422808 | None:intergenic |
| GTCTTGGCTAAACTTTGTGA+TGG | 0.583455 | 8.4:-71422230 | None:intergenic |
| TCAAAGGCACAACATCACTA+AGG | 0.583560 | 8.4:-71422774 | None:intergenic |
| GGATAGCAAAACAGGAGATG+TGG | 0.608354 | 8.4:+71422372 | MS.gene81054:CDS |
| ATAAGGAGCAGTTATGGCAA+AGG | 0.621792 | 8.4:-71422416 | None:intergenic |
| GATACTCAGCCACAAAACAT+CGG | 0.646194 | 8.4:+71422484 | MS.gene81054:CDS |
| AGTCAATAAGCACTACACCA+TGG | 0.646654 | 8.4:+71422647 | MS.gene81054:CDS |
| AGGGAAGTTACAACTTACCA+AGG | 0.684795 | 8.4:+71422297 | MS.gene81054:CDS |
| ATGCCATAACTACCTCAACA+GGG | 0.753652 | 8.4:+71422278 | MS.gene81054:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CATTGTTGTTATTAAAAACT+AGG | - | chr8.4:71422903-71422922 | None:intergenic | 20.0% |
| ! | CCAAAGATAAATAGACATAT+TGG | + | chr8.4:71422604-71422623 | MS.gene81054:CDS | 25.0% |
| ! | CCAATATGTCTATTTATCTT+TGG | - | chr8.4:71422607-71422626 | None:intergenic | 25.0% |
| !! | AGACACTTTTCATAATACTT+GGG | + | chr8.4:71422579-71422598 | MS.gene81054:CDS | 25.0% |
| !! | TAGACACTTTTCATAATACT+TGG | + | chr8.4:71422578-71422597 | MS.gene81054:CDS | 25.0% |
| !!! | CTTCATCTTGTTATTCAATA+AGG | + | chr8.4:71422180-71422199 | MS.gene81054:CDS | 25.0% |
| ATTCTGCTCCTATTCATATT+TGG | + | chr8.4:71422350-71422369 | MS.gene81054:CDS | 30.0% | |
| CAAAAGAATCTAATCTTCCA+AGG | + | chr8.4:71422253-71422272 | MS.gene81054:CDS | 30.0% | |
| CAGAACATGATATTCGTTAT+TGG | + | chr8.4:71422857-71422876 | MS.gene81054:CDS | 30.0% | |
| TAACAAGATGAAGACTGTTA+AGG | - | chr8.4:71422173-71422192 | None:intergenic | 30.0% | |
| TCACTAAGGATATAACTATC+AGG | - | chr8.4:71422763-71422782 | None:intergenic | 30.0% | |
| TCAGGAACAATATCTTTCAA+AGG | - | chr8.4:71422793-71422812 | None:intergenic | 30.0% | |
| TTCTGCTCCTATTCATATTT+GGG | + | chr8.4:71422351-71422370 | MS.gene81054:CDS | 30.0% | |
| TTGCTATCCCAAATATGAAT+AGG | - | chr8.4:71422361-71422380 | None:intergenic | 30.0% | |
| ! | AGATTAGATTCTTTTGGTCT+TGG | - | chr8.4:71422249-71422268 | None:intergenic | 30.0% |
| ! | CTTGGAAGATTAGATTCTTT+TGG | - | chr8.4:71422255-71422274 | None:intergenic | 30.0% |
| AGTGTAGTTATGTCTTATCC+AGG | + | chr8.4:71422733-71422752 | MS.gene81054:CDS | 35.0% | |
| ATATAACTATCAGGAAGTCC+TGG | - | chr8.4:71422754-71422773 | None:intergenic | 35.0% | |
| GAAAGATATTGTTCCTGAGT+GGG | + | chr8.4:71422795-71422814 | MS.gene81054:CDS | 35.0% | |
| GCTAAACTTTGTGATGGTAA+AGG | - | chr8.4:71422227-71422246 | None:intergenic | 35.0% | |
| TATAGTACCAATGTTGCAGA+TGG | + | chr8.4:71422436-71422455 | MS.gene81054:CDS | 35.0% | |
| TCTGCAACATTGGTACTATA+AGG | - | chr8.4:71422436-71422455 | None:intergenic | 35.0% | |
| TGAAAGATATTGTTCCTGAG+TGG | + | chr8.4:71422794-71422813 | MS.gene81054:CDS | 35.0% | |
| ! | CATATTTGGGATAGCAAAAC+AGG | + | chr8.4:71422364-71422383 | MS.gene81054:CDS | 35.0% |
| AAGGCAGTCAAGAACTCTAT+TGG | + | chr8.4:71422316-71422335 | MS.gene81054:CDS | 40.0% | |
| AAGTTGTAACTTCCCTGTTG+AGG | - | chr8.4:71422293-71422312 | None:intergenic | 40.0% | |
| AATAGAGTTCTTGACTGCCT+TGG | - | chr8.4:71422317-71422336 | None:intergenic | 40.0% | |
| AATGCCATAACTACCTCAAC+AGG | + | chr8.4:71422277-71422296 | MS.gene81054:CDS | 40.0% | |
| AGGGAAGTTACAACTTACCA+AGG | + | chr8.4:71422297-71422316 | MS.gene81054:CDS | 40.0% | |
| AGTCAATAAGCACTACACCA+TGG | + | chr8.4:71422647-71422666 | MS.gene81054:CDS | 40.0% | |
| ATAAGGAGCAGTTATGGCAA+AGG | - | chr8.4:71422419-71422438 | None:intergenic | 40.0% | |
| ATATTGTTCCTGAGTGGGTA+AGG | + | chr8.4:71422800-71422819 | MS.gene81054:CDS | 40.0% | |
| ATGCCATAACTACCTCAACA+GGG | + | chr8.4:71422278-71422297 | MS.gene81054:CDS | 40.0% | |
| GAGAGGCTAATGTTGTGATA+AGG | + | chr8.4:71422686-71422705 | MS.gene81054:CDS | 40.0% | |
| GATACTCAGCCACAAAACAT+CGG | + | chr8.4:71422484-71422503 | MS.gene81054:CDS | 40.0% | |
| GATGAAGACTGTTAAGGAGA+TGG | - | chr8.4:71422167-71422186 | None:intergenic | 40.0% | |
| GGTACTATAAGGAGCAGTTA+TGG | - | chr8.4:71422425-71422444 | None:intergenic | 40.0% | |
| GTCAATAAGCACTACACCAT+GGG | + | chr8.4:71422648-71422667 | MS.gene81054:CDS | 40.0% | |
| GTCTTGGCTAAACTTTGTGA+TGG | - | chr8.4:71422233-71422252 | None:intergenic | 40.0% | |
| TAATGTTTCAAGCTCTGTGC+AGG | + | chr8.4:71422924-71422943 | MS.gene81054:CDS | 40.0% | |
| TACCAATGTTGCAGATGGTT+TGG | + | chr8.4:71422441-71422460 | MS.gene81054:CDS | 40.0% | |
| ! | ACACCATGGGTTTTGCAAAA+TGG | + | chr8.4:71422661-71422680 | MS.gene81054:CDS | 40.0% |
| ! | CTTCCATTTTGCAAAACCCA+TGG | - | chr8.4:71422667-71422686 | None:intergenic | 40.0% |
| ! | GGTTTTGCAAAATGGAAGAG+AGG | + | chr8.4:71422669-71422688 | MS.gene81054:CDS | 40.0% |
| ! | TCAAAGGCACAACATCACTA+AGG | - | chr8.4:71422777-71422796 | None:intergenic | 40.0% |
| AGGTAGTTATGGCATTGCCT+TGG | - | chr8.4:71422273-71422292 | None:intergenic | 45.0% | |
| CTTCCCTGTTGAGGTAGTTA+TGG | - | chr8.4:71422284-71422303 | None:intergenic | 45.0% | |
| GGATAGCAAAACAGGAGATG+TGG | + | chr8.4:71422372-71422391 | MS.gene81054:CDS | 45.0% | |
| TTCTGCAAATTCTGCACCAG+TGG | - | chr8.4:71422842-71422861 | None:intergenic | 45.0% | |
| ! | TTTGGCCTTCTTCATTGCAC+CGG | + | chr8.4:71422459-71422478 | MS.gene81054:CDS | 45.0% |
| !!! | TTTTGTGGCTGAGTATCGAC+CGG | - | chr8.4:71422481-71422500 | None:intergenic | 45.0% |
| AAACCGATCCTTACCCACTC+AGG | - | chr8.4:71422811-71422830 | None:intergenic | 50.0% | |
| ATCGACCGGTGCAATGAAGA+AGG | - | chr8.4:71422467-71422486 | None:intergenic | 50.0% | |
| ATCGGTCGTGCAGGATTTCT+CGG | + | chr8.4:71422502-71422521 | MS.gene81054:CDS | 50.0% | |
| GGCCAAACCATCTGCAACAT+TGG | - | chr8.4:71422446-71422465 | None:intergenic | 50.0% | |
| GTTCCTGAGTGGGTAAGGAT+CGG | + | chr8.4:71422805-71422824 | MS.gene81054:CDS | 50.0% | |
| CCACAAAACATCGGTCGTGC+AGG | + | chr8.4:71422493-71422512 | MS.gene81054:CDS | 55.0% | |
| ! | ATCGGTTTCTCAGCAGCCAC+TGG | + | chr8.4:71422823-71422842 | MS.gene81054:CDS | 55.0% |
| ! | CCTGCACGACCGATGTTTTG+TGG | - | chr8.4:71422496-71422515 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 71422133 | 71422954 | 71422133 | ID=MS.gene81054 |
| chr8.4 | mRNA | 71422133 | 71422954 | 71422133 | ID=MS.gene81054.t1;Parent=MS.gene81054 |
| chr8.4 | exon | 71422133 | 71422954 | 71422133 | ID=MS.gene81054.t1.exon1;Parent=MS.gene81054.t1 |
| chr8.4 | CDS | 71422133 | 71422954 | 71422133 | ID=cds.MS.gene81054.t1;Parent=MS.gene81054.t1 |
| Gene Sequence |
| Protein sequence |
