Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81175.t1 | PNX75835.1 | 63.6 | 44 | 16 | 0 | 1 | 44 | 1 | 44 | 3.90E-06 | 61.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81175.t1 | A0A2K3LBB7 | 63.6 | 44 | 16 | 0 | 1 | 44 | 1 | 44 | 2.8e-06 | 61.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057406 | MS.gene81175 | 0.814553 | 1.45E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81175.t1 | MTR_2g044740 | 51.020 | 98 | 44 | 2 | 1 | 98 | 1 | 94 | 7.85e-15 | 65.5 |
| MS.gene81175.t1 | MTR_2g042470 | 37.864 | 103 | 55 | 2 | 1 | 99 | 1 | 98 | 1.74e-12 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAGGAGAAAATAATAAAA+AGG | 0.299777 | 6.1:-55286814 | MS.gene81175:CDS |
| GAAAAGAACACAAAGATAAA+AGG | 0.316710 | 6.1:+55287200 | None:intergenic |
| AGGAGGAGGTGGACAAAATC+AGG | 0.330426 | 6.1:-55286874 | MS.gene81175:CDS |
| CATACATTGTTTCCCATGTC+TGG | 0.366229 | 6.1:+55287136 | None:intergenic |
| GGATCAAGCTCAGGAGAAAA+TGG | 0.374472 | 6.1:-55286915 | MS.gene81175:CDS |
| GCTGGCCGGGAGGATTAAAA+AGG | 0.387508 | 6.1:-55286977 | MS.gene81175:CDS |
| AATGGACCGCTTGGCAAAGC+TGG | 0.395837 | 6.1:-55286995 | MS.gene81175:CDS |
| ATTGGTAAAGAAGGCCAAAA+AGG | 0.400206 | 6.1:-55286954 | MS.gene81175:CDS |
| GACCGCTTGGCAAAGCTGGC+CGG | 0.427719 | 6.1:-55286991 | MS.gene81175:CDS |
| ACCGCTTGGCAAAGCTGGCC+GGG | 0.446723 | 6.1:-55286990 | MS.gene81175:CDS |
| TTGGGATACAGAATTGGAAA+TGG | 0.458344 | 6.1:-55287013 | MS.gene81175:CDS |
| GAAGGAGTTGCGCAAGGAGG+AGG | 0.483849 | 6.1:-55286888 | MS.gene81175:CDS |
| GAACACAAAGATAAAAGGCT+TGG | 0.496731 | 6.1:+55287205 | None:intergenic |
| AGAATTGGAAATGGACCGCT+TGG | 0.497786 | 6.1:-55287004 | MS.gene81175:CDS |
| CTAATTCTTGTCAATGTTGT+GGG | 0.501759 | 6.1:-55287170 | MS.gene81175:CDS |
| GGAGTTGCGCAAGGAGGAGG+TGG | 0.504922 | 6.1:-55286885 | MS.gene81175:CDS |
| GGCTCAGGTGGATCAAGCTC+AGG | 0.505461 | 6.1:-55286924 | MS.gene81175:CDS |
| GAGCTTGATCCACCTGAGCC+CGG | 0.516745 | 6.1:+55286927 | None:intergenic |
| AAAGGTGAACCGGGCTCAGG+TGG | 0.518166 | 6.1:-55286936 | MS.gene81175:CDS |
| TCAGGAGAAAATGGCGGTGA+AGG | 0.543194 | 6.1:-55286906 | MS.gene81175:CDS |
| ACTAATATAACATAAAATCA+TGG | 0.545693 | 6.1:+55286775 | None:intergenic |
| GACAAAGAAAGTGCGACTGA+TGG | 0.553843 | 6.1:-55286843 | MS.gene81175:CDS |
| GCGACTGATGGAGGAAGAAG+AGG | 0.555550 | 6.1:-55286831 | MS.gene81175:CDS |
| ACTAATTCTTGTCAATGTTG+TGG | 0.555572 | 6.1:-55287171 | MS.gene81175:CDS |
| TTGATTTGTAGTAGATTCAA+CGG | 0.568580 | 6.1:-55287054 | MS.gene81175:intron |
| AAAGAAAGTGCGACTGATGG+AGG | 0.575733 | 6.1:-55286840 | MS.gene81175:CDS |
| GGAATTGAATCATCCAGACA+TGG | 0.592959 | 6.1:-55287149 | MS.gene81175:CDS |
| GGAGGAGGTGGACAAAATCA+GGG | 0.611095 | 6.1:-55286873 | MS.gene81175:CDS |
| GGCGGTGAAGGAGTTGCGCA+AGG | 0.611945 | 6.1:-55286894 | MS.gene81175:CDS |
| TCCCGGCCAGCTTTGCCAAG+CGG | 0.616564 | 6.1:+55286989 | None:intergenic |
| GCTTGGCAAAGCTGGCCGGG+AGG | 0.664993 | 6.1:-55286987 | MS.gene81175:CDS |
| GAATTGAATCATCCAGACAT+GGG | 0.689467 | 6.1:-55287148 | MS.gene81175:CDS |
| GGTGAAGGAGTTGCGCAAGG+AGG | 0.712010 | 6.1:-55286891 | MS.gene81175:CDS |
| TCAAGCTCAGGAGAAAATGG+CGG | 0.716902 | 6.1:-55286912 | MS.gene81175:CDS |
| GGAGGTGGACAAAATCAGGG+TGG | 0.749319 | 6.1:-55286870 | MS.gene81175:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAAATAATAAAAAGGATTA+TGG | - | chr6.1:55287181-55287200 | MS.gene81175:CDS | 15.0% |
| !! | AAGAAGTCAATACAAAAATT+GGG | - | chr6.1:55286957-55286976 | MS.gene81175:CDS | 20.0% |
| !! | AAGAGGAGAAAATAATAAAA+AGG | - | chr6.1:55287174-55287193 | MS.gene81175:CDS | 20.0% |
| !! | AATAATAAAAAGGATTATGG+AGG | - | chr6.1:55287184-55287203 | MS.gene81175:CDS | 20.0% |
| !! | TTAGATATTACAAATGAAGA+AGG | + | chr6.1:55286901-55286920 | None:intergenic | 20.0% |
| ! | GAAAAGAACACAAAGATAAA+AGG | + | chr6.1:55286791-55286810 | None:intergenic | 25.0% |
| ! | GAAGAAGTCAATACAAAAAT+TGG | - | chr6.1:55286956-55286975 | MS.gene81175:CDS | 25.0% |
| !! | TTAAAAAGGATTGGTAAAGA+AGG | - | chr6.1:55287025-55287044 | MS.gene81175:CDS | 25.0% |
| !!! | TTGATTTGTAGTAGATTCAA+CGG | - | chr6.1:55286934-55286953 | MS.gene81175:CDS | 25.0% |
| ACTAATTCTTGTCAATGTTG+TGG | - | chr6.1:55286817-55286836 | MS.gene81175:CDS | 30.0% | |
| ATAAAAAGGATTATGGAGGT+TGG | - | chr6.1:55287188-55287207 | MS.gene81175:CDS | 30.0% | |
| CAAAAATTGGGATACAGAAT+TGG | - | chr6.1:55286969-55286988 | MS.gene81175:CDS | 30.0% | |
| CTAATTCTTGTCAATGTTGT+GGG | - | chr6.1:55286818-55286837 | MS.gene81175:CDS | 30.0% | |
| TAAAAAGGATTATGGAGGTT+GGG | - | chr6.1:55287189-55287208 | MS.gene81175:CDS | 30.0% | |
| ATTGGTAAAGAAGGCCAAAA+AGG | - | chr6.1:55287034-55287053 | MS.gene81175:CDS | 35.0% | |
| GAACACAAAGATAAAAGGCT+TGG | + | chr6.1:55286786-55286805 | None:intergenic | 35.0% | |
| GAATTGAATCATCCAGACAT+GGG | - | chr6.1:55286840-55286859 | MS.gene81175:CDS | 35.0% | |
| TTGGGATACAGAATTGGAAA+TGG | - | chr6.1:55286975-55286994 | MS.gene81175:CDS | 35.0% | |
| CATACATTGTTTCCCATGTC+TGG | + | chr6.1:55286855-55286874 | None:intergenic | 40.0% | |
| GGAATTGAATCATCCAGACA+TGG | - | chr6.1:55286839-55286858 | MS.gene81175:CDS | 40.0% | |
| ! | CCAATCCTTTTTAATCCTCC+CGG | + | chr6.1:55287019-55287038 | None:intergenic | 40.0% |
| AGAAGGCCAAAAAGGTGAAC+CGG | - | chr6.1:55287042-55287061 | MS.gene81175:CDS | 45.0% | |
| AGAATTGGAAATGGACCGCT+TGG | - | chr6.1:55286984-55287003 | MS.gene81175:CDS | 45.0% | |
| CCGGGAGGATTAAAAAGGAT+TGG | - | chr6.1:55287016-55287035 | MS.gene81175:CDS | 45.0% | |
| GGATCAAGCTCAGGAGAAAA+TGG | - | chr6.1:55287073-55287092 | MS.gene81175:intron | 45.0% | |
| TCAAGCTCAGGAGAAAATGG+CGG | - | chr6.1:55287076-55287095 | MS.gene81175:intron | 45.0% | |
| ! | AAAGAAAGTGCGACTGATGG+AGG | - | chr6.1:55287148-55287167 | MS.gene81175:CDS | 45.0% |
| ! | GACAAAGAAAGTGCGACTGA+TGG | - | chr6.1:55287145-55287164 | MS.gene81175:CDS | 45.0% |
| AGGAGGAGGTGGACAAAATC+AGG | - | chr6.1:55287114-55287133 | MS.gene81175:intron | 50.0% | |
| GAAGGCCAAAAAGGTGAACC+GGG | - | chr6.1:55287043-55287062 | MS.gene81175:CDS | 50.0% | |
| GGAGGAGGTGGACAAAATCA+GGG | - | chr6.1:55287115-55287134 | MS.gene81175:intron | 50.0% | |
| TCAGGAGAAAATGGCGGTGA+AGG | - | chr6.1:55287082-55287101 | MS.gene81175:intron | 50.0% | |
| AATGGACCGCTTGGCAAAGC+TGG | - | chr6.1:55286993-55287012 | MS.gene81175:CDS | 55.0% | |
| CAAAAAGGTGAACCGGGCTC+AGG | - | chr6.1:55287049-55287068 | MS.gene81175:intron | 55.0% | |
| GCGACTGATGGAGGAAGAAG+AGG | - | chr6.1:55287157-55287176 | MS.gene81175:CDS | 55.0% | |
| GCTGGCCGGGAGGATTAAAA+AGG | - | chr6.1:55287011-55287030 | MS.gene81175:CDS | 55.0% | |
| GGAGGTGGACAAAATCAGGG+TGG | - | chr6.1:55287118-55287137 | MS.gene81175:intron | 55.0% | |
| ! | CTGAGCCCGGTTCACCTTTT+TGG | + | chr6.1:55287051-55287070 | None:intergenic | 55.0% |
| AAAGGTGAACCGGGCTCAGG+TGG | - | chr6.1:55287052-55287071 | MS.gene81175:intron | 60.0% | |
| GAAGGAGTTGCGCAAGGAGG+AGG | - | chr6.1:55287100-55287119 | MS.gene81175:intron | 60.0% | |
| GAGCTTGATCCACCTGAGCC+CGG | + | chr6.1:55287064-55287083 | None:intergenic | 60.0% | |
| GGCTCAGGTGGATCAAGCTC+AGG | - | chr6.1:55287064-55287083 | MS.gene81175:intron | 60.0% | |
| GGTGAAGGAGTTGCGCAAGG+AGG | - | chr6.1:55287097-55287116 | MS.gene81175:intron | 60.0% | |
| ACCGCTTGGCAAAGCTGGCC+GGG | - | chr6.1:55286998-55287017 | MS.gene81175:CDS | 65.0% | |
| GACCGCTTGGCAAAGCTGGC+CGG | - | chr6.1:55286997-55287016 | MS.gene81175:CDS | 65.0% | |
| GGAGTTGCGCAAGGAGGAGG+TGG | - | chr6.1:55287103-55287122 | MS.gene81175:intron | 65.0% | |
| GGCGGTGAAGGAGTTGCGCA+AGG | - | chr6.1:55287094-55287113 | MS.gene81175:intron | 65.0% | |
| TCCCGGCCAGCTTTGCCAAG+CGG | + | chr6.1:55287002-55287021 | None:intergenic | 65.0% | |
| GCTTGGCAAAGCTGGCCGGG+AGG | - | chr6.1:55287001-55287020 | MS.gene81175:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 55286776 | 55287234 | 55286776 | ID=MS.gene81175 |
| chr6.1 | mRNA | 55286776 | 55287234 | 55286776 | ID=MS.gene81175.t1;Parent=MS.gene81175 |
| chr6.1 | exon | 55287141 | 55287234 | 55287141 | ID=MS.gene81175.t1.exon1;Parent=MS.gene81175.t1 |
| chr6.1 | CDS | 55287141 | 55287234 | 55287141 | ID=cds.MS.gene81175.t1;Parent=MS.gene81175.t1 |
| chr6.1 | exon | 55286776 | 55287065 | 55286776 | ID=MS.gene81175.t1.exon2;Parent=MS.gene81175.t1 |
| chr6.1 | CDS | 55286776 | 55287065 | 55286776 | ID=cds.MS.gene81175.t1;Parent=MS.gene81175.t1 |
| Gene Sequence |
| Protein sequence |
