Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81269.t1 | AET00863.2 | 100 | 62 | 0 | 0 | 1 | 62 | 1 | 62 | 1.70E-23 | 117.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81269.t1 | G7K4U3 | 100.0 | 62 | 0 | 0 | 1 | 62 | 1 | 62 | 1.2e-23 | 117.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049255 | MS.gene81269 | 0.819294 | 1.25E-52 | -1.69E-46 |
| MS.gene049341 | MS.gene81269 | 0.806268 | 8.93E-50 | -1.69E-46 |
| MS.gene051328 | MS.gene81269 | 0.836073 | 1.16E-56 | -1.69E-46 |
| MS.gene058055 | MS.gene81269 | 0.813782 | 2.15E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81269.t1 | MTR_5g096760 | 100.000 | 62 | 0 | 0 | 1 | 62 | 1 | 62 | 1.25e-39 | 124 |
| MS.gene81269.t1 | MTR_5g096780 | 86.154 | 65 | 6 | 1 | 1 | 62 | 1 | 65 | 1.45e-32 | 106 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81269.t1 | AT3G13275 | 63.636 | 44 | 15 | 1 | 1 | 44 | 1 | 43 | 3.80e-14 | 60.5 |
Find 17 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGCGGAGGAAGGCCGTTCT+TGG | 0.245467 | 5.2:-81135901 | MS.gene81269:CDS |
| ATCGGAATTCTGTTTGGATT+TGG | 0.287774 | 5.2:-81135981 | MS.gene81269:CDS |
| ATCACGATCGGAATTCTGTT+TGG | 0.387585 | 5.2:-81135987 | MS.gene81269:CDS |
| GAACGGTGTCTTTGACTTTG+TGG | 0.441601 | 5.2:+81135940 | None:intergenic |
| GGCGGAGGAAGGCCGTTCTT+GGG | 0.459430 | 5.2:-81135900 | MS.gene81269:CDS |
| GTTCATTACTCTGATTCTTA+CGG | 0.479388 | 5.2:-81135921 | MS.gene81269:CDS |
| TACTCTGATTCTTACGGCGG+AGG | 0.514379 | 5.2:-81135915 | MS.gene81269:CDS |
| CTGATTCTTACGGCGGAGGA+AGG | 0.537452 | 5.2:-81135911 | MS.gene81269:CDS |
| TTAATCAAGATGTGTTGTCC+AGG | 0.568505 | 5.2:-81136050 | None:intergenic |
| ACAATACTGAACTAGAACAA+TGG | 0.587324 | 5.2:+81135864 | None:intergenic |
| ATTGTGGTTGTGATCACGAT+CGG | 0.620795 | 5.2:-81135999 | MS.gene81269:CDS |
| GCTATGCATATGCTTGATTG+TGG | 0.622895 | 5.2:-81136015 | MS.gene81269:CDS |
| CTGAACTAGAACAATGGTGG+TGG | 0.629807 | 5.2:+81135870 | None:intergenic |
| CATTACTCTGATTCTTACGG+CGG | 0.653791 | 5.2:-81135918 | MS.gene81269:CDS |
| GGTGGCGCATAACCCAAGAA+CGG | 0.667489 | 5.2:+81135888 | None:intergenic |
| ATACTGAACTAGAACAATGG+TGG | 0.667708 | 5.2:+81135867 | None:intergenic |
| GTAAGAATCAGAGTAATGAA+CGG | 0.707351 | 5.2:+81135923 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTAAGAATCAGAGTAATGAA+CGG | + | chr5.2:81135996-81136015 | None:intergenic | 30.0% | |
| GTTCATTACTCTGATTCTTA+CGG | - | chr5.2:81135995-81136014 | MS.gene81269:CDS | 30.0% | |
| ATCGGAATTCTGTTTGGATT+TGG | - | chr5.2:81135935-81135954 | MS.gene81269:CDS | 35.0% | |
| !! | GGATTTGGAGTTTTCAAGAA+TGG | - | chr5.2:81135950-81135969 | MS.gene81269:CDS | 35.0% |
| ATCACGATCGGAATTCTGTT+TGG | - | chr5.2:81135929-81135948 | MS.gene81269:CDS | 40.0% | |
| ATTGTGGTTGTGATCACGAT+CGG | - | chr5.2:81135917-81135936 | MS.gene81269:CDS | 40.0% | |
| CATTACTCTGATTCTTACGG+CGG | - | chr5.2:81135998-81136017 | MS.gene81269:CDS | 40.0% | |
| GCTATGCATATGCTTGATTG+TGG | - | chr5.2:81135901-81135920 | MS.gene81269:CDS | 40.0% | |
| ! | GAACGGTGTCTTTGACTTTG+TGG | + | chr5.2:81135979-81135998 | None:intergenic | 45.0% |
| TACTCTGATTCTTACGGCGG+AGG | - | chr5.2:81136001-81136020 | MS.gene81269:CDS | 50.0% | |
| ! | CATAGCGTGCAGATTTTGCC+TGG | + | chr5.2:81135887-81135906 | None:intergenic | 50.0% |
| CTGATTCTTACGGCGGAGGA+AGG | - | chr5.2:81136005-81136024 | MS.gene81269:CDS | 55.0% | |
| GGTGGCGCATAACCCAAGAA+CGG | + | chr5.2:81136031-81136050 | None:intergenic | 55.0% | |
| GGCGGAGGAAGGCCGTTCTT+GGG | - | chr5.2:81136016-81136035 | MS.gene81269:CDS | 65.0% | |
| CGGCGGAGGAAGGCCGTTCT+TGG | - | chr5.2:81136015-81136034 | MS.gene81269:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 81135875 | 81136063 | 81135875 | ID=MS.gene81269 |
| chr5.2 | mRNA | 81135875 | 81136063 | 81135875 | ID=MS.gene81269.t1;Parent=MS.gene81269 |
| chr5.2 | exon | 81135875 | 81136063 | 81135875 | ID=MS.gene81269.t1.exon1;Parent=MS.gene81269.t1 |
| chr5.2 | CDS | 81135875 | 81136063 | 81135875 | ID=cds.MS.gene81269.t1;Parent=MS.gene81269.t1 |
| Gene Sequence |
| Protein sequence |
