AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81294

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81294.t1 MTR_5g096310 99.481 385 2 0 1 385 1 385 0.0 773
MS.gene81294.t1 MTR_2g038410 60.963 374 142 3 3 373 1 373 2.86e-168 475
MS.gene81294.t1 MTR_2g094640 29.683 347 216 5 55 380 10 349 4.71e-40 145
MS.gene81294.t1 MTR_3g094270 28.213 319 210 4 73 385 28 333 4.02e-38 140
MS.gene81294.t1 MTR_4g058860 27.485 342 219 3 55 371 10 347 3.32e-37 138
MS.gene81294.t1 MTR_3g094260 28.339 307 201 4 73 373 28 321 5.68e-33 126
MS.gene81294.t1 MTR_3g095130 28.939 311 204 7 62 365 20 320 1.94e-28 114
MS.gene81294.t1 MTR_1g026590 32.602 319 202 5 59 369 15 328 4.61e-28 113
MS.gene81294.t1 MTR_5g004620 31.833 311 200 6 63 365 21 327 6.68e-28 112
MS.gene81294.t1 MTR_1g098240 27.393 303 201 4 46 338 4 297 1.53e-25 105
MS.gene81294.t1 MTR_8g092190 27.134 328 216 5 62 375 20 338 1.50e-22 97.8
MS.gene81294.t1 MTR_3g095130 27.046 281 188 7 92 365 1 271 5.39e-20 89.4
MS.gene81294.t1 MTR_3g072380 23.585 318 224 5 68 373 27 337 1.67e-18 86.3
MS.gene81294.t1 MTR_1g030320 22.222 324 233 5 68 383 26 338 5.20e-17 81.6
MS.gene81294.t1 MTR_8g090130 29.758 289 174 9 68 338 24 301 4.25e-13 69.7
MS.gene81294.t1 MTR_2g070400 25.786 318 205 13 69 366 24 330 4.62e-13 70.1
MS.gene81294.t1 MTR_4g101860 26.241 282 195 7 68 339 65 343 6.43e-13 69.7
MS.gene81294.t1 MTR_3g072380 26.282 156 108 2 68 219 27 179 1.08e-12 67.8
MS.gene81294.t1 MTR_3g070000 25.850 294 198 10 68 350 37 321 1.21e-11 65.5
MS.gene81294.t1 MTR_0011s0320 25.070 355 218 14 40 372 1 329 2.88e-11 64.7
MS.gene81294.t1 MTR_7g110380 24.606 317 216 9 69 371 28 335 3.52e-11 64.7
MS.gene81294.t1 MTR_4g018760 27.682 289 182 11 68 339 25 303 5.02e-11 63.5
MS.gene81294.t1 MTR_3g462070 25.773 291 199 9 60 338 14 299 6.37e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81294.t1 AT5G55950 79.893 373 72 2 1 370 1 373 0.0 608
MS.gene81294.t1 AT5G55950 85.911 291 40 1 1 290 1 291 0.0 514
MS.gene81294.t1 AT5G55950 78.914 313 64 1 60 370 1 313 8.90e-178 498
MS.gene81294.t1 AT5G55950 78.644 295 61 1 78 370 1 295 1.41e-166 469
MS.gene81294.t1 AT5G57100 64.897 339 116 1 7 342 12 350 9.10e-162 459
MS.gene81294.t1 AT5G57100 64.808 287 98 1 7 290 12 298 5.86e-136 390
MS.gene81294.t1 AT5G57100 63.043 230 82 1 7 233 12 241 1.80e-102 304
MS.gene81294.t1 AT5G57100 62.996 227 84 0 147 373 40 266 3.45e-95 286
MS.gene81294.t1 AT2G30460 32.432 333 185 8 73 374 28 351 4.71e-42 151
MS.gene81294.t1 AT2G30460 32.432 333 185 8 73 374 28 351 4.71e-42 151
MS.gene81294.t1 AT1G06890 32.456 342 202 9 43 370 2 328 2.60e-41 149
MS.gene81294.t1 AT1G06890 32.456 342 202 9 43 370 2 328 3.24e-41 149
MS.gene81294.t1 AT1G06890 32.456 342 202 9 43 370 2 328 5.14e-41 148
MS.gene81294.t1 AT1G06890 32.456 342 202 9 43 370 2 328 5.14e-41 148
MS.gene81294.t1 AT2G30460 32.797 311 175 7 95 374 23 330 1.16e-40 147
MS.gene81294.t1 AT2G30460 32.797 311 175 7 95 374 23 330 1.16e-40 147
MS.gene81294.t1 AT2G30460 32.797 311 175 7 95 374 23 330 1.16e-40 147
MS.gene81294.t1 AT2G28315 30.363 303 200 3 55 353 10 305 1.83e-35 133
MS.gene81294.t1 AT2G28315 30.032 313 206 4 45 353 2 305 7.00e-35 131
MS.gene81294.t1 AT5G42420 33.013 312 200 4 59 363 18 327 2.12e-31 122
MS.gene81294.t1 AT1G21070 30.448 335 217 6 59 382 18 347 9.96e-31 120
MS.gene81294.t1 AT4G09810 27.003 337 228 7 47 373 5 333 9.07e-30 117
MS.gene81294.t1 AT1G76670 29.598 348 231 5 44 380 2 346 4.42e-29 116
MS.gene81294.t1 AT1G76670 29.598 348 231 5 44 380 2 346 4.42e-29 116
MS.gene81294.t1 AT1G34020 27.019 322 217 7 62 373 20 333 2.49e-28 114
MS.gene81294.t1 AT1G34020 27.019 322 217 7 62 373 20 333 2.49e-28 114
MS.gene81294.t1 AT1G34020 27.019 322 217 7 62 373 20 333 2.49e-28 114
MS.gene81294.t1 AT2G30460 32.000 200 118 5 73 263 28 218 5.16e-24 102
MS.gene81294.t1 AT4G39390 26.627 338 235 6 41 373 4 333 3.12e-22 96.7
MS.gene81294.t1 AT4G39390 26.627 338 235 6 41 373 4 333 3.12e-22 96.7
MS.gene81294.t1 AT4G39390 26.786 336 233 6 43 373 2 329 5.12e-22 96.3
MS.gene81294.t1 AT4G09810 26.715 277 188 6 107 373 5 276 5.68e-21 92.0
MS.gene81294.t1 AT1G77610 24.458 323 223 5 68 382 21 330 1.37e-20 92.0
MS.gene81294.t1 AT1G21870 23.457 324 227 5 68 383 27 337 2.06e-19 89.0
MS.gene81294.t1 AT4G09810 28.846 208 144 2 47 254 5 208 4.06e-19 85.9
MS.gene81294.t1 AT1G53660 28.115 313 196 13 69 366 48 346 5.43e-14 72.8
MS.gene81294.t1 AT1G53660 28.115 313 196 13 69 366 30 328 6.41e-14 72.4
MS.gene81294.t1 AT1G53660 27.667 300 190 12 80 366 9 294 2.39e-13 70.5
MS.gene81294.t1 AT3G14410 28.076 317 196 14 69 364 28 333 2.23e-12 68.2
MS.gene81294.t1 AT5G25400 24.534 322 214 11 70 372 32 343 2.58e-12 67.8
MS.gene81294.t1 AT1G12500 26.241 282 195 7 68 339 71 349 4.42e-12 67.4
MS.gene81294.t1 AT3G17430 26.549 339 200 16 69 378 25 343 3.90e-11 64.3
MS.gene81294.t1 AT3G17430 26.549 339 200 16 69 378 25 343 3.90e-11 64.3
MS.gene81294.t1 AT3G10290 26.690 281 193 7 68 338 70 347 8.69e-11 63.2
MS.gene81294.t1 AT3G11320 27.562 283 190 9 68 339 23 301 9.94e-11 62.8

Find 78 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGCTATAGTTGCAATTATA+TGG 0.196565 5.2:-80802255 MS.gene81294:CDS
TGAACAAATGTGAGGAAAAT+TGG 0.206523 5.2:+80803333 None:intergenic
GATTTAGAGTTCAATTTATT+TGG 0.233562 5.2:-80802277 MS.gene81294:CDS
CGCATTATCCTTCAACTTCA+TGG 0.275264 5.2:-80803529 MS.gene81294:CDS
TTCTAGGAGCTTTGATGCCT+TGG 0.276040 5.2:-80802043 MS.gene81294:CDS
TGGTCAGGTGCCTTGGCATT+AGG 0.292303 5.2:-80801930 MS.gene81294:CDS
ATTTGCACTTGGTGCTGTTA+TGG 0.295321 5.2:-80803217 MS.gene81294:CDS
TTACATCCCACTTGAATGAT+AGG 0.344646 5.2:+80802003 None:intergenic
CTTGGTTGTTATGGGATTGT+TGG 0.362653 5.2:-80804239 None:intergenic
GACAGTGATGCAGGAGAAAC+AGG 0.365115 5.2:-80804175 MS.gene81294:intron
GGTCAGGTGCCTTGGCATTA+GGG 0.373196 5.2:-80801929 MS.gene81294:intron
GATCATCATGGCAAACAAAT+TGG 0.383428 5.2:-80803496 MS.gene81294:intron
GTCCGCAATATCTTTGTTGT+TGG 0.384372 5.2:+80803559 None:intergenic
GAACAAATGTGAGGAAAATT+GGG 0.386839 5.2:+80803334 None:intergenic
AAGACCTGTGTTATTCTGTT+GGG 0.387128 5.2:-80801728 MS.gene81294:CDS
ATTTACAGCAGCAAGGAAAC+TGG 0.392992 5.2:-80802195 MS.gene81294:CDS
TGTTACTCCAACAATTGTTC+TGG 0.395251 5.2:-80802524 MS.gene81294:CDS
ATATCTTTGTTGTTGGCGCT+TGG 0.395651 5.2:+80803566 None:intergenic
TTCAACTTCATGGTTGCTGT+TGG 0.397088 5.2:-80803519 MS.gene81294:CDS
TCAACTTCATGGTTGCTGTT+GGG 0.398882 5.2:-80803518 MS.gene81294:CDS
CTGTTATTCGACTCAGATCC+GGG 0.403312 5.2:-80801698 MS.gene81294:CDS
GCTTGGCTCCTTCCGAGGTT+CGG 0.411178 5.2:+80803583 None:intergenic
CCTAGAAAGAAAACTGTGAT+AGG 0.412694 5.2:+80802059 None:intergenic
TCAGACAACAATGTTATTGT+TGG 0.416058 5.2:+80801502 None:intergenic
ACACCAGCCTCAAGTATAAC+AGG 0.419149 5.2:-80803173 MS.gene81294:intron
TAACAGCAATCTTAGCCATT+TGG 0.422409 5.2:+80802542 None:intergenic
AACTCTGCCAGAACAATTGT+TGG 0.424008 5.2:+80802517 None:intergenic
CTTACAGTGGTCAGGTGCCT+TGG 0.424187 5.2:-80801937 MS.gene81294:CDS
TCTTTGTTGTCTTCACTAGC+TGG 0.443142 5.2:+80801542 None:intergenic
CAGTGTTGGTTTCTACCAAA+TGG 0.447856 5.2:-80802557 MS.gene81294:CDS
GCTTTGGCTGCTGTATCAGC+TGG 0.447867 5.2:-80802322 MS.gene81294:CDS
TTCTCTTCTATATTTGCACT+TGG 0.450424 5.2:-80803228 MS.gene81294:CDS
CCGGGGATTGTGAGCATTGG+CGG 0.454725 5.2:-80801680 MS.gene81294:CDS
GCAAGGAAACTGGACAGCTT+TGG 0.461956 5.2:-80802185 MS.gene81294:CDS
GGGATTGTTGGATTCACTGT+TGG 0.464531 5.2:-80804227 MS.gene81294:CDS
TCTGTTATTCGACTCAGATC+CGG 0.468414 5.2:-80801699 MS.gene81294:CDS
GGAGCGGTTGTTGCTCTCAC+TGG 0.471179 5.2:-80801659 MS.gene81294:CDS
GGATTGTTGGATTCACTGTT+GGG 0.478433 5.2:-80804226 MS.gene81294:CDS
TAAGACCTGTGTTATTCTGT+TGG 0.483155 5.2:-80801729 MS.gene81294:CDS
TGTTGCTCTCACTGGAATGT+CGG 0.489922 5.2:-80801651 MS.gene81294:CDS
CCGCCAATGCTCACAATCCC+CGG 0.490390 5.2:+80801680 None:intergenic
AAAAGAAAAGACAGTGATGC+AGG 0.490754 5.2:-80804184 MS.gene81294:CDS
CACTTGAATGATAGGACTCC+TGG 0.491316 5.2:+80802011 None:intergenic
AGTGCAAATATAGAAGAGAA+TGG 0.515498 5.2:+80803231 None:intergenic
AACAGCAACCATGAAGTTGA+AGG 0.516911 5.2:+80803521 None:intergenic
GTTGAAGGCAAACTATGCTT+CGG 0.526876 5.2:+80801575 None:intergenic
TTGGATTCACTGTTGGGAGA+TGG 0.530762 5.2:-80804220 MS.gene81294:CDS
TTGGCGCTTGGCTCCTTCCG+AGG 0.534697 5.2:+80803578 None:intergenic
GATCCGGGGATTGTGAGCAT+TGG 0.537864 5.2:-80801683 MS.gene81294:CDS
GAAATTATCAGGTAAAGCAC+TGG 0.545102 5.2:-80803637 MS.gene81294:intron
CTTTATTGTTGATAGGTGAT+GGG 0.545367 5.2:-80803375 MS.gene81294:intron
TGATAGGTGATGGGAAGAGT+TGG 0.547749 5.2:-80803366 MS.gene81294:intron
TTTCTCATTGTAGATTGATG+TGG 0.552469 5.2:-80802091 MS.gene81294:intron
ACCTGTGTTATTCTGTTGGG+AGG 0.560653 5.2:-80801725 MS.gene81294:CDS
AATGAGCTCCGAACCTCGGA+AGG 0.566484 5.2:-80803591 MS.gene81294:CDS
TTGTTCACTACATCACTGCA+TGG 0.573296 5.2:-80803317 MS.gene81294:CDS
AACAATAACATTGTTGTCTG+AGG 0.579088 5.2:-80801500 None:intergenic
TGGTCTAATTTACAGCAGCA+AGG 0.584781 5.2:-80802202 MS.gene81294:CDS
TTGAAGGATAATGCGACCAC+TGG 0.591814 5.2:+80803537 None:intergenic
CTACAATGAGCTCCGAACCT+CGG 0.592720 5.2:-80803595 MS.gene81294:CDS
CGCCAACAACAAAGATATTG+CGG 0.594240 5.2:-80803561 MS.gene81294:CDS
CAGGAGTCCTATCATTCAAG+TGG 0.594492 5.2:-80802010 MS.gene81294:CDS
ATGCCTTGGATTGATCCACC+AGG 0.606051 5.2:-80802029 MS.gene81294:CDS
ACATGAACCTGTTATACTTG+AGG 0.618701 5.2:+80803166 None:intergenic
GATTGTGAGCATTGGCGGAG+CGG 0.623842 5.2:-80801675 MS.gene81294:CDS
GAACCTGTTATACTTGAGGC+TGG 0.638511 5.2:+80803170 None:intergenic
AAAGTGCCTTGAAAATTGCA+AGG 0.638843 5.2:+80803289 None:intergenic
ACTCCTGGTGGATCAATCCA+AGG 0.644949 5.2:+80802026 None:intergenic
GTAGTCTTACCCTAATGCCA+AGG 0.645323 5.2:+80801920 None:intergenic
TGTAGCAGTTGCAACTGTGT+CGG 0.648797 5.2:-80802299 MS.gene81294:CDS
GCCTCCCAACAGAATAACAC+AGG 0.658463 5.2:+80801724 None:intergenic
AGGAGTCCTATCATTCAAGT+GGG 0.665530 5.2:-80802009 MS.gene81294:CDS
GGTTGCTGTTGGGATCATCA+TGG 0.682153 5.2:-80803508 MS.gene81294:CDS
TGTTATTCGACTCAGATCCG+GGG 0.684228 5.2:-80801697 MS.gene81294:CDS
ACAAAGATATTGCGGACCAG+TGG 0.685300 5.2:-80803553 MS.gene81294:CDS
TTGAATGATAGGACTCCTGG+TGG 0.704189 5.2:+80802014 None:intergenic
TGATGTAGTGAACAAATGTG+AGG 0.721156 5.2:+80803325 None:intergenic
ACCACTGTAAGAGAAAACCG+AGG 0.773883 5.2:+80801949 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATAATTTATTTCCTTGAA+TGG - chr5.2:80802957-80802976 MS.gene81294:intron 15.0%
!! ATTATGTAATTCAATTATGA+TGG + chr5.2:80802018-80802037 None:intergenic 15.0%
!! TCCAAAAAATATTTCATATT+TGG - chr5.2:80801925-80801944 MS.gene81294:intron 15.0%
!!! AAATATGAAATATTTTTTGG+AGG + chr5.2:80801926-80801945 None:intergenic 15.0%
!!! ACCAAATATGAAATATTTTT+TGG + chr5.2:80801929-80801948 None:intergenic 15.0%
!!! AGACCATAAAATTTTATTTA+TGG + chr5.2:80803515-80803534 None:intergenic 15.0%
!!! TAATTTTACAAAAGAGAAAT+TGG + chr5.2:80803594-80803613 None:intergenic 15.0%
!!! TTTTCTGTTTTTTCAAATTT+TGG - chr5.2:80801812-80801831 MS.gene81294:intron 15.0%
!! AAAAACCATTTCTTCAAAAA+AGG - chr5.2:80803249-80803268 MS.gene81294:CDS 20.0%
!! AAAGATTATGTTAATTGTCA+TGG - chr5.2:80802849-80802868 MS.gene81294:intron 20.0%
!! ATATTATTATCAATCAATGC+AGG - chr5.2:80803941-80803960 MS.gene81294:intron 20.0%
!! ATGTTTCAAATTTGTAAATG+TGG - chr5.2:80802722-80802741 MS.gene81294:intron 20.0%
!! CATAAACCATTTCAAATATA+TGG - chr5.2:80803311-80803330 MS.gene81294:CDS 20.0%
!! GATATAACTTTATTGTTGAT+AGG - chr5.2:80802349-80802368 MS.gene81294:intron 20.0%
!! GTGCATAAAAAATAATTGTA+TGG + chr5.2:80803349-80803368 None:intergenic 20.0%
!! TATTATTATCAATCAATGCA+GGG - chr5.2:80803942-80803961 MS.gene81294:intron 20.0%
!! TATTTGTGTTCAATTCTTAA+TGG - chr5.2:80801703-80801722 MS.gene81294:CDS 20.0%
!! TTAAAAGTCAATGACTAATT+TGG + chr5.2:80802649-80802668 None:intergenic 20.0%
!! TTGGTATGAAAAATAAACAA+AGG + chr5.2:80802630-80802649 None:intergenic 20.0%
!!! AAAATTTTATTTATGGCACT+TGG + chr5.2:80803508-80803527 None:intergenic 20.0%
!!! GATTTAGAGTTCAATTTATT+TGG - chr5.2:80803454-80803473 MS.gene81294:intron 20.0%
!!! GTGCCATAAATAAAATTTTA+TGG - chr5.2:80803509-80803528 MS.gene81294:CDS 20.0%
!!! TCTTTTCCATATATTTGAAA+TGG + chr5.2:80803320-80803339 None:intergenic 20.0%
!!! TTGTCATGGATTTTTTTTAA+AGG - chr5.2:80802863-80802882 MS.gene81294:intron 20.0%
!!! TTTTTAAAGGTTAATTGTGA+TGG - chr5.2:80802876-80802895 MS.gene81294:intron 20.0%
! AAAATATGCCTAAATATCAG+CGG - chr5.2:80801756-80801775 MS.gene81294:CDS 25.0%
! AAACATAATGACTGAACATT+AGG + chr5.2:80802708-80802727 None:intergenic 25.0%
! AATGATGCTGATCATAAATA+GGG - chr5.2:80802035-80802054 MS.gene81294:CDS 25.0%
! ATTAGGTCAGTAATGAAAAT+TGG + chr5.2:80802691-80802710 None:intergenic 25.0%
! CATAGATGAAGAATTAAGTT+TGG - chr5.2:80803004-80803023 MS.gene81294:intron 25.0%
! GCACTAATTAATCATGTAAT+TGG - chr5.2:80803365-80803384 MS.gene81294:intron 25.0%
! GTAAGGTATAAATCTGAATT+TGG - chr5.2:80801856-80801875 MS.gene81294:intron 25.0%
! TAATGATGCTGATCATAAAT+AGG - chr5.2:80802034-80802053 MS.gene81294:CDS 25.0%
!! AGACTTAATCTTTTATTGCT+TGG + chr5.2:80801900-80801919 None:intergenic 25.0%
!! AGTAGAATTTTGTGTCAAAA+AGG + chr5.2:80803875-80803894 None:intergenic 25.0%
!! GTAGAATTTTGTGTCAAAAA+GGG + chr5.2:80803874-80803893 None:intergenic 25.0%
!!! TATTTGTTATGCTAAGAGTA+AGG - chr5.2:80801839-80801858 MS.gene81294:intron 25.0%
!!! TGTCAACATGGATTTAATTT+TGG + chr5.2:80802794-80802813 None:intergenic 25.0%
!!! TGTTGTGTTTTGTTTAAGTA+AGG - chr5.2:80803903-80803922 MS.gene81294:intron 25.0%
!!! TTTGACCTTTTTTGAAGAAA+TGG + chr5.2:80803257-80803276 None:intergenic 25.0%
AAATATCAGCGGTTTGAAAA+GGG - chr5.2:80801767-80801786 MS.gene81294:CDS 30.0%
AAATCTTACTTGACCATTCA+AGG + chr5.2:80802973-80802992 None:intergenic 30.0%
AACATACATGCAATATGAGA+AGG + chr5.2:80802273-80802292 None:intergenic 30.0%
AACCTAATTGAAACTTAAGC+TGG - chr5.2:80803095-80803114 MS.gene81294:intron 30.0%
AGTGCAAATATAGAAGAGAA+TGG + chr5.2:80802503-80802522 None:intergenic 30.0%
ATGGAACATCTAATGAAAGT+AGG + chr5.2:80801999-80802018 None:intergenic 30.0%
GAACAAATGTGAGGAAAATT+GGG + chr5.2:80802400-80802419 None:intergenic 30.0%
GTGCTATAGTTGCAATTATA+TGG - chr5.2:80803476-80803495 MS.gene81294:intron 30.0%
TAAATATCAGCGGTTTGAAA+AGG - chr5.2:80801766-80801785 MS.gene81294:CDS 30.0%
TCAGACAACAATGTTATTGT+TGG + chr5.2:80804232-80804251 None:intergenic 30.0%
TCATCATGGTTGAAATTATC+AGG - chr5.2:80802083-80802102 MS.gene81294:intron 30.0%
TGAACAAATGTGAGGAAAAT+TGG + chr5.2:80802401-80802420 None:intergenic 30.0%
TTCTCTTCTATATTTGCACT+TGG - chr5.2:80802503-80802522 MS.gene81294:CDS 30.0%
TTTCTCATTGTAGATTGATG+TGG - chr5.2:80803640-80803659 MS.gene81294:intron 30.0%
TTTGCTCTAACATGTTTATC+AGG - chr5.2:80802589-80802608 MS.gene81294:intron 30.0%
! ACTTTATTGTTGATAGGTGA+TGG - chr5.2:80802355-80802374 MS.gene81294:intron 30.0%
! CTTTATTGTTGATAGGTGAT+GGG - chr5.2:80802356-80802375 MS.gene81294:intron 30.0%
! GAATGGTCAAGTAAGATTTT+TGG - chr5.2:80802974-80802993 MS.gene81294:intron 30.0%
! TTTTCAAACCGCTGATATTT+AGG + chr5.2:80801767-80801786 None:intergenic 30.0%
AAAAGAAAAGACAGTGATGC+AGG - chr5.2:80801547-80801566 MS.gene81294:CDS 35.0%
ACATGAACCTGTTATACTTG+AGG + chr5.2:80802568-80802587 None:intergenic 35.0%
CACCAGCTTAAGTTTCAATT+AGG + chr5.2:80803100-80803119 None:intergenic 35.0%
CCTAGAAAGAAAACTGTGAT+AGG + chr5.2:80803675-80803694 None:intergenic 35.0%
GAAATTATCAGGTAAAGCAC+TGG - chr5.2:80802094-80802113 MS.gene81294:intron 35.0%
GATCATCATGGCAAACAAAT+TGG - chr5.2:80802235-80802254 MS.gene81294:CDS 35.0%
TAACAGCAATCTTAGCCATT+TGG + chr5.2:80803192-80803211 None:intergenic 35.0%
TAATTGGAATGCAATGATCC+AGG - chr5.2:80803381-80803400 MS.gene81294:intron 35.0%
TATTCCTGACTCTCTATGAT+TGG - chr5.2:80801673-80801692 MS.gene81294:CDS 35.0%
TGATGTAGTGAACAAATGTG+AGG + chr5.2:80802409-80802428 None:intergenic 35.0%
TGTTACTCCAACAATTGTTC+TGG - chr5.2:80803207-80803226 MS.gene81294:CDS 35.0%
TTACATCCCACTTGAATGAT+AGG + chr5.2:80803731-80803750 None:intergenic 35.0%
! AAAGTGCCTTGAAAATTGCA+AGG + chr5.2:80802445-80802464 None:intergenic 35.0%
! AAGACCTGTGTTATTCTGTT+GGG - chr5.2:80804003-80804022 MS.gene81294:intron 35.0%
! CCTATCACAGTTTTCTTTCT+AGG - chr5.2:80803672-80803691 MS.gene81294:intron 35.0%
! GATTCTCCTTGCAATTTTCA+AGG - chr5.2:80802436-80802455 MS.gene81294:intron 35.0%
! TAAGACCTGTGTTATTCTGT+TGG - chr5.2:80804002-80804021 MS.gene81294:intron 35.0%
!! ATTTTGATATCTGCTCTCCT+CGG - chr5.2:80803765-80803784 MS.gene81294:intron 35.0%
!! TGACTTTGTTGTTGTCAACA+TGG + chr5.2:80802806-80802825 None:intergenic 35.0%
!! TGCAATTTTCAAGGCACTTT+CGG - chr5.2:80802445-80802464 MS.gene81294:intron 35.0%
!! TGGTGAAGTTTTGTCTAGTT+TGG - chr5.2:80803115-80803134 MS.gene81294:intron 35.0%
!!! AATGGAGTTGTTTTAGATGG+AGG + chr5.2:80802485-80802504 None:intergenic 35.0%
!!! GAGAATGGAGTTGTTTTAGA+TGG + chr5.2:80802488-80802507 None:intergenic 35.0%
AAAGCCAATCATAGAGAGTC+AGG + chr5.2:80801680-80801699 None:intergenic 40.0%
AACAGCAACCATGAAGTTGA+AGG + chr5.2:80802213-80802232 None:intergenic 40.0%
AACTCTGCCAGAACAATTGT+TGG + chr5.2:80803217-80803236 None:intergenic 40.0%
AGGAGTCCTATCATTCAAGT+GGG - chr5.2:80803722-80803741 MS.gene81294:intron 40.0%
ATTTACAGCAGCAAGGAAAC+TGG - chr5.2:80803536-80803555 MS.gene81294:CDS 40.0%
CATAGAGAGTCAGGAATACA+TGG + chr5.2:80801671-80801690 None:intergenic 40.0%
CATGATGAGAACACTGCATT+AGG + chr5.2:80802071-80802090 None:intergenic 40.0%
CGCATTATCCTTCAACTTCA+TGG - chr5.2:80802202-80802221 MS.gene81294:CDS 40.0%
CGCCAACAACAAAGATATTG+CGG - chr5.2:80802170-80802189 MS.gene81294:intron 40.0%
CTAATGCAGTGTTCTCATCA+TGG - chr5.2:80802069-80802088 MS.gene81294:CDS 40.0%
GAACTGAACTGTCAGCAAAA+AGG + chr5.2:80803151-80803170 None:intergenic 40.0%
GAACTGTCAGCAAAAAGGAA+AGG + chr5.2:80803146-80803165 None:intergenic 40.0%
GGATTGTTGGATTCACTGTT+GGG - chr5.2:80801505-80801524 MS.gene81294:CDS 40.0%
GTTGAAGGCAAACTATGCTT+CGG + chr5.2:80804159-80804178 None:intergenic 40.0%
TCTGTTATTCGACTCAGATC+CGG - chr5.2:80804032-80804051 MS.gene81294:intron 40.0%
TGGTCTAATTTACAGCAGCA+AGG - chr5.2:80803529-80803548 MS.gene81294:CDS 40.0%
TTCAACTTCATGGTTGCTGT+TGG - chr5.2:80802212-80802231 MS.gene81294:CDS 40.0%
TTGTTCACTACATCACTGCA+TGG - chr5.2:80802414-80802433 MS.gene81294:intron 40.0%
! GAATGCAATGATCCAGGTTT+TGG - chr5.2:80803387-80803406 MS.gene81294:intron 40.0%
! GTCCGCAATATCTTTGTTGT+TGG + chr5.2:80802175-80802194 None:intergenic 40.0%
! TCAACTTCATGGTTGCTGTT+GGG - chr5.2:80802213-80802232 MS.gene81294:CDS 40.0%
! TCTTTGTTGTCTTCACTAGC+TGG + chr5.2:80804192-80804211 None:intergenic 40.0%
!! ATATCTTTGTTGTTGGCGCT+TGG + chr5.2:80802168-80802187 None:intergenic 40.0%
!! ATTTGCACTTGGTGCTGTTA+TGG - chr5.2:80802514-80802533 MS.gene81294:CDS 40.0%
!! CAGTGTTGGTTTCTACCAAA+TGG - chr5.2:80803174-80803193 MS.gene81294:CDS 40.0%
!!! AATGATCCAGGTTTTGGCTT+TGG - chr5.2:80803393-80803412 MS.gene81294:intron 40.0%
!!! GCTACGACTCATGTTGTTTT+AGG - chr5.2:80803973-80803992 MS.gene81294:intron 40.0%
ACAAAGATATTGCGGACCAG+TGG - chr5.2:80802178-80802197 MS.gene81294:intron 45.0%
ACACCAGCCTCAAGTATAAC+AGG - chr5.2:80802558-80802577 MS.gene81294:intron 45.0%
ACCACTGTAAGAGAAAACCG+AGG + chr5.2:80803785-80803804 None:intergenic 45.0%
AGTCAGGAATACATGGAAGG+AGG + chr5.2:80801664-80801683 None:intergenic 45.0%
CACTTGAATGATAGGACTCC+TGG + chr5.2:80803723-80803742 None:intergenic 45.0%
CAGGAGTCCTATCATTCAAG+TGG - chr5.2:80803721-80803740 MS.gene81294:intron 45.0%
CAGTTCAGTTCATGCAGTGT+TGG - chr5.2:80803160-80803179 MS.gene81294:intron 45.0%
CTGTTATTCGACTCAGATCC+GGG - chr5.2:80804033-80804052 MS.gene81294:intron 45.0%
GAACCTGTTATACTTGAGGC+TGG + chr5.2:80802564-80802583 None:intergenic 45.0%
GAGAGTCAGGAATACATGGA+AGG + chr5.2:80801667-80801686 None:intergenic 45.0%
GGGATTGTTGGATTCACTGT+TGG - chr5.2:80801504-80801523 MS.gene81294:CDS 45.0%
TGTAGCAGTTGCAACTGTGT+CGG - chr5.2:80803432-80803451 MS.gene81294:intron 45.0%
TGTTATTCGACTCAGATCCG+GGG - chr5.2:80804034-80804053 MS.gene81294:intron 45.0%
TGTTGCTCTCACTGGAATGT+CGG - chr5.2:80804080-80804099 MS.gene81294:intron 45.0%
TTGAAGGATAATGCGACCAC+TGG + chr5.2:80802197-80802216 None:intergenic 45.0%
TTGAATGATAGGACTCCTGG+TGG + chr5.2:80803720-80803739 None:intergenic 45.0%
TTGGATTCACTGTTGGGAGA+TGG - chr5.2:80801511-80801530 MS.gene81294:CDS 45.0%
! ACCTGTGTTATTCTGTTGGG+AGG - chr5.2:80804006-80804025 MS.gene81294:intron 45.0%
! GCTGTTATGGCTTTTGCATC+TGG - chr5.2:80802527-80802546 MS.gene81294:CDS 45.0%
! GGTTTTCTCTTACAGTGGTC+AGG - chr5.2:80803786-80803805 MS.gene81294:intron 45.0%
! GTAGTCTTACCCTAATGCCA+AGG + chr5.2:80803814-80803833 None:intergenic 45.0%
! TCCTCGGTTTTCTCTTACAG+TGG - chr5.2:80803781-80803800 MS.gene81294:intron 45.0%
! TTCTAGGAGCTTTGATGCCT+TGG - chr5.2:80803688-80803707 MS.gene81294:intron 45.0%
!! GTTTTAGATGGAGGAGACAC+AGG + chr5.2:80802476-80802495 None:intergenic 45.0%
!! TGATAGGTGATGGGAAGAGT+TGG - chr5.2:80802365-80802384 MS.gene81294:intron 45.0%
!!! GCTGGTTTTTGCTGTGTTGA+AGG + chr5.2:80804174-80804193 None:intergenic 45.0%
!!! ATTTGATTTATATAAAAAAT+TGG - chr5.2:80801632-80801651 MS.gene81294:CDS 5.0%
ATGCCTTGGATTGATCCACC+AGG - chr5.2:80803702-80803721 MS.gene81294:intron 50.0%
CTACAATGAGCTCCGAACCT+CGG - chr5.2:80802136-80802155 MS.gene81294:intron 50.0%
GACAGTGATGCAGGAGAAAC+AGG - chr5.2:80801556-80801575 MS.gene81294:CDS 50.0%
GCAAGGAAACTGGACAGCTT+TGG - chr5.2:80803546-80803565 MS.gene81294:CDS 50.0%
GCCTCCCAACAGAATAACAC+AGG + chr5.2:80804010-80804029 None:intergenic 50.0%
! ACTCCTGGTGGATCAATCCA+AGG + chr5.2:80803708-80803727 None:intergenic 50.0%
! GGTTGCTGTTGGGATCATCA+TGG - chr5.2:80802223-80802242 MS.gene81294:CDS 50.0%
AATGAGCTCCGAACCTCGGA+AGG - chr5.2:80802140-80802159 MS.gene81294:intron 55.0%
CAGCAGCCAAAGCCAAAACC+TGG + chr5.2:80803402-80803421 None:intergenic 55.0%
CTTACAGTGGTCAGGTGCCT+TGG - chr5.2:80803794-80803813 MS.gene81294:intron 55.0%
GATCCGGGGATTGTGAGCAT+TGG - chr5.2:80804048-80804067 MS.gene81294:intron 55.0%
GCTTTGGCTGCTGTATCAGC+TGG - chr5.2:80803409-80803428 MS.gene81294:intron 55.0%
GGTCAGGTGCCTTGGCATTA+GGG - chr5.2:80803802-80803821 MS.gene81294:intron 55.0%
TGGTCAGGTGCCTTGGCATT+AGG - chr5.2:80803801-80803820 MS.gene81294:intron 55.0%
!! GATTGTGAGCATTGGCGGAG+CGG - chr5.2:80804056-80804075 MS.gene81294:intron 55.0%
CCGCCAATGCTCACAATCCC+CGG + chr5.2:80804054-80804073 None:intergenic 60.0%
GCTTGGCTCCTTCCGAGGTT+CGG + chr5.2:80802151-80802170 None:intergenic 60.0%
GGAGCGGTTGTTGCTCTCAC+TGG - chr5.2:80804072-80804091 MS.gene81294:intron 60.0%
!! CCGGGGATTGTGAGCATTGG+CGG - chr5.2:80804051-80804070 MS.gene81294:intron 60.0%
TTGGCGCTTGGCTCCTTCCG+AGG + chr5.2:80802156-80802175 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 80801502 80804251 80801502 ID=MS.gene81294
chr5.2 mRNA 80801502 80804251 80801502 ID=MS.gene81294.t1;Parent=MS.gene81294
chr5.2 exon 80804176 80804251 80804176 ID=MS.gene81294.t1.exon1;Parent=MS.gene81294.t1
chr5.2 CDS 80804176 80804251 80804176 ID=cds.MS.gene81294.t1;Parent=MS.gene81294.t1
chr5.2 exon 80803497 80803648 80803497 ID=MS.gene81294.t1.exon2;Parent=MS.gene81294.t1
chr5.2 CDS 80803497 80803648 80803497 ID=cds.MS.gene81294.t1;Parent=MS.gene81294.t1
chr5.2 exon 80803174 80803382 80803174 ID=MS.gene81294.t1.exon3;Parent=MS.gene81294.t1
chr5.2 CDS 80803174 80803382 80803174 ID=cds.MS.gene81294.t1;Parent=MS.gene81294.t1
chr5.2 exon 80802483 80802576 80802483 ID=MS.gene81294.t1.exon4;Parent=MS.gene81294.t1
chr5.2 CDS 80802483 80802576 80802483 ID=cds.MS.gene81294.t1;Parent=MS.gene81294.t1
chr5.2 exon 80802184 80802350 80802184 ID=MS.gene81294.t1.exon5;Parent=MS.gene81294.t1
chr5.2 CDS 80802184 80802350 80802184 ID=cds.MS.gene81294.t1;Parent=MS.gene81294.t1
chr5.2 exon 80801930 80802100 80801930 ID=MS.gene81294.t1.exon6;Parent=MS.gene81294.t1
chr5.2 CDS 80801930 80802100 80801930 ID=cds.MS.gene81294.t1;Parent=MS.gene81294.t1
chr5.2 exon 80801502 80801790 80801502 ID=MS.gene81294.t1.exon7;Parent=MS.gene81294.t1
chr5.2 CDS 80801502 80801790 80801502 ID=cds.MS.gene81294.t1;Parent=MS.gene81294.t1
Gene Sequence

>MS.gene81294

ATGGGATTGTTGGATTCACTGTTGGGAGATGGACGAAAATTTATCAAAAGAAAAGACAGTGATGCAGGAGAAACAGGTTTTTTTTCTTTCTGAATTTCTTAGTTCATATTTGATTGTAATTGAGTTTTGTATTTGATTTATATAAAAAATTGGTTTTTGCCTCCTTCCATGTATTCCTGACTCTCTATGATTGGCTTTTCATATTTGTGTTCAATTCTTAATGGATTTATCAAAAAATAATCTGATTCTGTTTTAAAATATGCCTAAATATCAGCGGTTTGAAAAGGGATTATTAGTCTTTTGAGTGTTATTTTCTGTTTTTTCAAATTTTGGATGATATTTGTTATGCTAAGAGTAAGGTATAAATCTGAATTTGGATTATATGTTACACTCAACCAAGCAATAAAAGATTAAGTCTTCTCCTCCAAAAAATATTTCATATTTGGTTTGCACAGTCTAAATCATAAGTTTGTAACATTACTTTCTTTTTCTCTCCTACTTTCATTAGATGTTCCATCATAATTGAATTACATAATGATGCTGATCATAAATAGGGTTTTTTAAAACCTAATGCAGTGTTCTCATCATGGTTGAAATTATCAGGTAAAGCACTGGAAGAACTAAGATCTTCTCTCTACAATGAGCTCCGAACCTCGGAAGGAGCCAAGCGCCAACAACAAAGATATTGCGGACCAGTGGTCGCATTATCCTTCAACTTCATGGTTGCTGTTGGGATCATCATGGCAAACAAATTGGTCTGTACAAATTCCTTCTCATATTGCATGTATGTTATTTTTATCATTGCTATGACATATTAGCAAGAATGAAATACTTCTCTTGAAGTTTTGATATAACTTTATTGTTGATAGGTGATGGGAAGAGTTGGCTTCAACTTCCCAATTTTCCTCACATTTGTTCACTACATCACTGCATGGATTCTCCTTGCAATTTTCAAGGCACTTTCGGTGCTTCCTGTGTCTCCTCCATCTAAAACAACTCCATTCTCTTCTATATTTGCACTTGGTGCTGTTATGGCTTTTGCATCTGGTCTTGCAAACACCAGCCTCAAGTATAACAGGTTCATGTTTTTGCTCTAACATGTTTATCAGGCAAAGTTGCATCAGTCCTTTGTTTATTTTTCATACCAAATTAGTCATTGACTTTTAATAATGTTTAGACTGTTGAACCAATTTTCATTACTGACCTAATGTTCAGTCATTATGTTTCAAATTTGTAAATGTGGTCATTGAATGATATATATACTATAAAGAATGTTTCAGTTCTCAGCACCAAAATTAAATCCATGTTGACAACAACAAAGTCACAATCATATATTAAAACGAAAAAAAAGATTATGTTAATTGTCATGGATTTTTTTTAAAGGTTAATTGTGATGGATTAGATATTATAATAGTCATTATGATGTATTTTAAATGTTATCATGATGAATAGAAGATATAATTTATTTCCTTGAATGGTCAAGTAAGATTTTTGGTAACGTGCATAGATGAAGAATTAAGTTTGGTCTAATATTGTTGATGTAATGATGTTTTAAACATAAATGACTAACTTATGATAAAAAGAAGATAAAAGAACCTAATTGAAACTTAAGCTGGTGAAGTTTTGTCTAGTTTGGTGCTTCCTTTCCTTTTTGCTGACAGTTCAGTTCATGCAGTGTTGGTTTCTACCAAATGGCTAAGATTGCTGTTACTCCAACAATTGTTCTGGCAGAGTTCATACTTTTCAGAAAAACCATTTCTTCAAAAAAGGTCAAAAATAAGCTCTTCAGCTTCTTTTTATTCTCTCTTTCATAAACCATTTCAAATATATGGAAAAGAACTATTCCATACAATTATTTTTTATGCACTAATTAATCATGTAATTGGAATGCAATGATCCAGGTTTTGGCTTTGGCTGCTGTATCAGCTGGTGTAGCAGTTGCAACTGTGTCGGATTTAGAGTTCAATTTATTTGGTGCTATAGTTGCAATTATATGGATAATTCCAAGTGCCATAAATAAAATTTTATGGTCTAATTTACAGCAGCAAGGAAACTGGACAGCTTTGGCGTAAGTATTAGCAAACACTATCCAATTTCTCTTTTGTAAAATTAAGTTGTCAAAAACTAGACTTAAAAACTTTCTCATTGTAGATTGATGTGGAAGACTACACCTATCACAGTTTTCTTTCTAGGAGCTTTGATGCCTTGGATTGATCCACCAGGAGTCCTATCATTCAAGTGGGATGTAAACAACTCATCTGCAATTTTGATATCTGCTCTCCTCGGTTTTCTCTTACAGTGGTCAGGTGCCTTGGCATTAGGGTAAGACTACTTTCAAGTTTCAATAAAAATAAATAAATAGTGATACACCCTTTTTGACACAAAATTCTACTTCTAATAGTGTTGTGTTTTGTTTAAGTAAGGTTAAGATTTGCTAAGATATTATTATCAATCAATGCAGGGCAACTTCTGCTACGACTCATGTTGTTTTAGGACAGTTTAAGACCTGTGTTATTCTGTTGGGAGGCTATCTGTTATTCGACTCAGATCCGGGGATTGTGAGCATTGGCGGAGCGGTTGTTGCTCTCACTGGAATGTCGGTTTACACAACATTTAACTTGCAAGAGTCTCAAGAAAATACAAGCAAGCAGCTTCCGAAGCATAGTTTGCCTTCAACACAGCAAAAACCAGCTAGTGAAGACAACAAAGATTTTAGTGTAAATATTACCAACAATAACATTGTTGTCTGA
Protein sequence

>MS.gene81294.t1

MGLLDSLLGDGRKFIKRKDSDAGETGKALEELRSSLYNELRTSEGAKRQQQRYCGPVVALSFNFMVAVGIIMANKLVMGRVGFNFPIFLTFVHYITAWILLAIFKALSVLPVSPPSKTTPFSSIFALGAVMAFASGLANTSLKYNSVGFYQMAKIAVTPTIVLAEFILFRKTISSKKVLALAAVSAGVAVATVSDLEFNLFGAIVAIIWIIPSAINKILWSNLQQQGNWTALALMWKTTPITVFFLGALMPWIDPPGVLSFKWDVNNSSAILISALLGFLLQWSGALALGATSATTHVVLGQFKTCVILLGGYLLFDSDPGIVSIGGAVVALTGMSVYTTFNLQESQENTSKQLPKHSLPSTQQKPASEDNKDFSVNITNNNIVV