Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81369.t1 | XP_003617738.1 | 84.1 | 391 | 54 | 1 | 1 | 391 | 1 | 383 | 1.90E-112 | 416 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81369.t1 | G7K4I1 | 84.1 | 391 | 54 | 1 | 1 | 391 | 1 | 383 | 1.4e-112 | 416.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene81369 | 0.801667 | 8.08E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81369.t1 | MTR_5g094910 | 88.747 | 391 | 36 | 1 | 1 | 391 | 1 | 383 | 0.0 | 644 |
| MS.gene81369.t1 | MTR_5g094920 | 63.764 | 356 | 75 | 6 | 75 | 391 | 197 | 537 | 4.92e-131 | 387 |
| MS.gene81369.t1 | MTR_5g094920 | 49.593 | 246 | 52 | 5 | 1 | 213 | 1 | 207 | 1.63e-55 | 191 |
| MS.gene81369.t1 | MTR_5g094960 | 50.740 | 473 | 144 | 11 | 1 | 390 | 1 | 467 | 5.04e-118 | 351 |
| MS.gene81369.t1 | MTR_5g094990 | 49.263 | 475 | 149 | 13 | 1 | 390 | 1 | 468 | 5.98e-107 | 323 |
| MS.gene81369.t1 | MTR_5g094850 | 43.393 | 507 | 143 | 13 | 3 | 391 | 1 | 481 | 2.05e-86 | 270 |
| MS.gene81369.t1 | MTR_5g094850 | 42.075 | 530 | 140 | 15 | 3 | 391 | 1 | 504 | 3.47e-82 | 260 |
| MS.gene81369.t1 | MTR_5g094950 | 40.357 | 560 | 162 | 13 | 1 | 391 | 1 | 557 | 7.66e-73 | 237 |
| MS.gene81369.t1 | MTR_5g095390 | 38.549 | 441 | 147 | 18 | 3 | 391 | 1 | 369 | 4.25e-52 | 178 |
| MS.gene81369.t1 | MTR_5g094970 | 41.577 | 279 | 92 | 4 | 36 | 243 | 14 | 292 | 2.17e-40 | 146 |
| MS.gene81369.t1 | MTR_5g095050 | 37.872 | 235 | 94 | 5 | 42 | 243 | 167 | 382 | 6.01e-26 | 109 |
| MS.gene81369.t1 | MTR_5g095050 | 30.855 | 269 | 110 | 8 | 1 | 199 | 1 | 263 | 5.81e-11 | 64.3 |
| MS.gene81369.t1 | MTR_5g077500 | 37.013 | 154 | 80 | 3 | 98 | 246 | 96 | 237 | 7.72e-14 | 71.6 |
| MS.gene81369.t1 | MTR_5g095370 | 36.478 | 159 | 68 | 6 | 1 | 137 | 1 | 148 | 2.52e-12 | 66.2 |
| MS.gene81369.t1 | MTR_1g071620 | 51.807 | 83 | 30 | 2 | 186 | 258 | 106 | 188 | 4.49e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 62 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATGAAATGAATGCTATTTA+TGG | 0.157301 | 5.2:-80052335 | MS.gene81369:CDS |
| TGAATTAGAGTCTGAAATTT+TGG | 0.200999 | 5.2:-80061082 | MS.gene81369:CDS |
| GATTAAAACTGGTGAGTTAA+AGG | 0.223103 | 5.2:-80060857 | MS.gene81369:CDS |
| TGATGACCCAGGTTCGATTT+TGG | 0.247518 | 5.2:+80061404 | None:intergenic |
| GGAAACAATGAAGAAGAATT+TGG | 0.257020 | 5.2:-80060836 | MS.gene81369:CDS |
| TAAATTAGAGCACGAGGTTT+TGG | 0.288164 | 5.2:-80060989 | MS.gene81369:CDS |
| GCTGAAATTAAGATTAAAAC+TGG | 0.338214 | 5.2:-80060868 | MS.gene81369:CDS |
| CGGACGGTTGAGATATTACT+TGG | 0.354529 | 5.2:-80060778 | MS.gene81369:CDS |
| CGTCAGAAACATCCTCTTCC+TGG | 0.357072 | 5.2:+80060680 | None:intergenic |
| GTTAACCGCATTTGCAAACT+TGG | 0.363844 | 5.2:+80052253 | None:intergenic |
| GTAGATGCTCATCGCACTCT+TGG | 0.411937 | 5.2:-80060643 | MS.gene81369:CDS |
| GGGAACAGTGAACATGAAAA+AGG | 0.418775 | 5.2:-80052423 | MS.gene81369:CDS |
| TTCAAGTTTGTTGCTAGGGT+TGG | 0.426251 | 5.2:+80061374 | None:intergenic |
| TATCTTCAAGTTTGTTGCTA+GGG | 0.435539 | 5.2:+80061370 | None:intergenic |
| TTGAAGGAGAAACTTGTTGA+TGG | 0.453707 | 5.2:-80061141 | MS.gene81369:CDS |
| CATCGTTGTTGTCGTTATCA+AGG | 0.462013 | 5.2:+80052384 | None:intergenic |
| GGAGGGTGATAAGAATGATT+TGG | 0.467049 | 5.2:-80060890 | MS.gene81369:CDS |
| TTATCTTCAAGTTTGTTGCT+AGG | 0.469484 | 5.2:+80061369 | None:intergenic |
| GGTTGAAGTTAAGAATTTGA+AGG | 0.471513 | 5.2:-80061157 | MS.gene81369:CDS |
| AAAGAAGAAATGTATGAGAC+AGG | 0.472260 | 5.2:-80061228 | MS.gene81369:CDS |
| ACAAAAGAAGAACAATGAAC+TGG | 0.473393 | 5.2:-80061181 | MS.gene81369:CDS |
| ACAAAGCAAGGTTCGTGTAA+TGG | 0.481653 | 5.2:-80060911 | MS.gene81369:CDS |
| GACATTAAATGAAGAGATGG+TGG | 0.484309 | 5.2:-80061052 | MS.gene81369:CDS |
| CTTGCTCTGTAGCTGAGTAC+TGG | 0.490258 | 5.2:+80060612 | None:intergenic |
| GACGCTCAAGATGCATCATC+AGG | 0.492956 | 5.2:-80060589 | MS.gene81369:intron |
| GAAATGTATGAGACAGGTAA+TGG | 0.503863 | 5.2:-80061222 | MS.gene81369:CDS |
| GAATGACTGGAAGAGTTTCA+AGG | 0.511598 | 5.2:-80060732 | MS.gene81369:CDS |
| GACTGGAAGAGTTTCAAGGT+TGG | 0.512261 | 5.2:-80060728 | MS.gene81369:CDS |
| ATCTTAACAAGATCATGAAT+AGG | 0.517050 | 5.2:+80061345 | None:intergenic |
| ATCATCAGAGGAAGAAGAAG+TGG | 0.517134 | 5.2:+80052190 | None:intergenic |
| GAGAGTAGGGATTTGAGACT+CGG | 0.520311 | 5.2:-80061309 | MS.gene81369:CDS |
| AGTTTGTTGCTAGGGTTGGT+AGG | 0.527539 | 5.2:+80061378 | None:intergenic |
| GCTAGTGATGAACTTGGAGT+TGG | 0.533733 | 5.2:+80052290 | None:intergenic |
| ATTCAACCCAAAATCGAACC+TGG | 0.539946 | 5.2:-80061411 | MS.gene81369:CDS |
| ACGACAACAACGATGATGAA+TGG | 0.543622 | 5.2:-80052376 | MS.gene81369:CDS |
| TTCTTGATAATCTTACAAGA+AGG | 0.548649 | 5.2:-80052160 | MS.gene81369:CDS |
| ACTCGGAGAGGAGTTTCACT+TGG | 0.549729 | 5.2:-80061292 | MS.gene81369:CDS |
| GAGGACATTAAATGAAGAGA+TGG | 0.556544 | 5.2:-80061055 | MS.gene81369:CDS |
| TGAACTGCTAGTGATGAACT+TGG | 0.561399 | 5.2:+80052284 | None:intergenic |
| AGGAACCAAGTTTGCAAATG+CGG | 0.569154 | 5.2:-80052258 | MS.gene81369:CDS |
| ATTAAATGAAGAGATGGTGG+AGG | 0.581644 | 5.2:-80061049 | MS.gene81369:CDS |
| ATCAGAGGAAGAAGAAGTGG+AGG | 0.583434 | 5.2:+80052193 | None:intergenic |
| AAGCAAGGTTCGTGTAATGG+AGG | 0.588505 | 5.2:-80060908 | MS.gene81369:CDS |
| TGGAATTGCTGTGAAGAATG+AGG | 0.597222 | 5.2:-80052315 | MS.gene81369:CDS |
| TTCACTTGGAAAGACTCTCA+AGG | 0.600450 | 5.2:-80061278 | MS.gene81369:CDS |
| TCATCACTAGCAGTTCAACT+AGG | 0.607456 | 5.2:-80052278 | MS.gene81369:CDS |
| AGCAAGGTTCGTGTAATGGA+GGG | 0.616655 | 5.2:-80060907 | MS.gene81369:CDS |
| TCGTGATGAGGTACAAAGCA+AGG | 0.624053 | 5.2:-80060923 | MS.gene81369:CDS |
| CAGTACTCAGCTACAGAGCA+AGG | 0.625882 | 5.2:-80060611 | MS.gene81369:CDS |
| AAGAAACAATGAATCATCAG+AGG | 0.630095 | 5.2:+80052178 | None:intergenic |
| TGAGAAAAGAAAATGATAAG+CGG | 0.633648 | 5.2:-80060798 | MS.gene81369:CDS |
| GTTGTGCTAAGAGAAATGAT+TGG | 0.634304 | 5.2:-80061012 | MS.gene81369:CDS |
| TTGGTAGGTGATGATGACCC+AGG | 0.634407 | 5.2:+80061393 | None:intergenic |
| AAAAGAAAATGATAAGCGGA+CGG | 0.636762 | 5.2:-80060794 | MS.gene81369:CDS |
| TTCAACCCAAAATCGAACCT+GGG | 0.641655 | 5.2:-80061410 | MS.gene81369:CDS |
| GGACGGTTGAGATATTACTT+GGG | 0.657606 | 5.2:-80060777 | MS.gene81369:CDS |
| GATGAATGGTGTATGTCTCA+AGG | 0.659079 | 5.2:-80052362 | MS.gene81369:CDS |
| AAAGAAGAACAATGAACTGG+AGG | 0.659967 | 5.2:-80061178 | MS.gene81369:CDS |
| GATTGGTAAATTAGAGCACG+AGG | 0.662647 | 5.2:-80060995 | MS.gene81369:CDS |
| TAGGGATTTGAGACTCGGAG+AGG | 0.690796 | 5.2:-80061304 | MS.gene81369:CDS |
| TAATCTTACAAGAAGGTCTG+CGG | 0.693035 | 5.2:-80052153 | MS.gene81369:CDS |
| TGATAGCAATGCTCGTGATG+AGG | 0.725632 | 5.2:-80060935 | MS.gene81369:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 80052124 | 80061439 | 80052124 | ID=MS.gene81369 |
| chr5.2 | mRNA | 80052124 | 80061439 | 80052124 | ID=MS.gene81369.t1;Parent=MS.gene81369 |
| chr5.2 | exon | 80060590 | 80061439 | 80060590 | ID=MS.gene81369.t1.exon1;Parent=MS.gene81369.t1 |
| chr5.2 | CDS | 80060590 | 80061439 | 80060590 | ID=cds.MS.gene81369.t1;Parent=MS.gene81369.t1 |
| chr5.2 | exon | 80052124 | 80052449 | 80052124 | ID=MS.gene81369.t1.exon2;Parent=MS.gene81369.t1 |
| chr5.2 | CDS | 80052124 | 80052449 | 80052124 | ID=cds.MS.gene81369.t1;Parent=MS.gene81369.t1 |
| Gene Sequence |
| Protein sequence |
