AlfalfaGEDB | MS.gene81575

M. sativa cultivar XinJiangDaYe / MS.gene81575

KEGG: -
GO: -
PFAM: PF03140.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81575.t1	XP_024639458.1	72.2	176	48	1	2	177	280	454	4.60E-61	244.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81575.t1	Q9SD53	26.5	181	127	5	3	178	285	464	3.4e-12	73.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81575.t1	A0A396HXR1	72.2	176	48	1	2	177	280	454	3.3e-61	244.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051719	MS.gene81575	0.803607	3.22E-49	-1.69E-46
MS.gene051720	MS.gene81575	0.807803	4.23E-50	-1.69E-46
MS.gene05423	MS.gene81575	0.820374	7.10E-53	-1.69E-46
MS.gene054801	MS.gene81575	0.838725	2.43E-57	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81575.t1	MTR_5g086260	77.966	177	39	0	1	177	139	315	5.28e-97	283
MS.gene81575.t1	MTR_5g086340	65.714	175	59	1	1	175	264	437	1.08e-74	231
MS.gene81575.t1	MTR_5g087580	60.571	175	68	1	3	177	48	221	5.69e-72	216
MS.gene81575.t1	MTR_8g042830	55.135	185	73	2	1	176	236	419	2.43e-64	203
MS.gene81575.t1	MTR_5g016570	46.857	175	91	2	2	175	358	531	2.34e-39	140
MS.gene81575.t1	MTR_7g011980	35.795	176	110	3	2	175	172	346	3.70e-33	120
MS.gene81575.t1	MTR_6g010850	37.572	173	105	3	2	172	179	350	7.13e-33	120
MS.gene81575.t1	MTR_6g011050	38.690	168	100	3	2	167	239	405	2.41e-31	117
MS.gene81575.t1	MTR_6g010920	37.278	169	102	4	2	167	238	405	4.17e-31	116
MS.gene81575.t1	MTR_7g060310	38.857	175	102	2	1	171	315	488	4.53e-31	117
MS.gene81575.t1	MTR_6g011060	37.079	178	105	4	2	175	249	423	8.25e-31	115
MS.gene81575.t1	MTR_7g059480	40.341	176	96	4	1	170	601	773	1.70e-30	117
MS.gene81575.t1	MTR_7g059525	39.884	173	99	2	2	170	112	283	5.48e-30	111
MS.gene81575.t1	MTR_6g009590	36.158	177	110	3	2	176	241	416	9.72e-30	113
MS.gene81575.t1	MTR_0024s0080	36.932	176	108	3	2	175	236	410	3.41e-29	111
MS.gene81575.t1	MTR_7g060840	38.953	172	100	2	3	170	262	432	6.77e-29	110
MS.gene81575.t1	MTR_2g035640	33.133	166	109	2	2	166	180	344	8.59e-29	109
MS.gene81575.t1	MTR_6g009580	35.838	173	108	3	2	172	65	236	1.61e-28	106
MS.gene81575.t1	MTR_6g009610	35.795	176	110	3	2	175	241	415	7.84e-28	107
MS.gene81575.t1	MTR_6g010810	36.158	177	109	4	2	175	237	412	1.04e-27	107
MS.gene81575.t1	MTR_7g060330	38.857	175	101	2	1	171	316	488	1.23e-27	108
MS.gene81575.t1	MTR_6g010890	34.483	174	105	4	2	167	250	422	1.58e-27	107
MS.gene81575.t1	MTR_7g060080	35.260	173	107	2	2	170	20	191	2.57e-27	102
MS.gene81575.t1	MTR_7g059540	38.150	173	102	2	2	170	295	466	2.65e-27	107
MS.gene81575.t1	MTR_7g060260	38.506	174	102	2	2	171	255	427	3.24e-27	106
MS.gene81575.t1	MTR_7g059540	38.150	173	102	2	2	170	304	475	3.47e-27	106
MS.gene81575.t1	MTR_3g107590	34.463	177	113	3	2	176	251	426	2.43e-26	103
MS.gene81575.t1	MTR_6g009170	37.634	186	105	6	2	178	245	428	3.17e-26	103
MS.gene81575.t1	MTR_8g042170	32.979	188	108	4	2	171	271	458	5.88e-26	103
MS.gene81575.t1	MTR_8g042400	32.979	188	108	4	2	171	158	345	5.46e-25	99.4
MS.gene81575.t1	MTR_8g037923	34.225	187	97	5	2	166	284	466	1.94e-24	99.0
MS.gene81575.t1	MTR_8g037920	34.091	176	92	4	11	166	234	405	2.65e-24	98.2
MS.gene81575.t1	MTR_7g060020	34.783	184	100	4	2	178	265	435	1.72e-23	96.3
MS.gene81575.t1	MTR_7g059520	35.632	174	97	4	2	170	9	172	1.92e-23	91.7
MS.gene81575.t1	MTR_7g060320	39.655	174	100	2	1	170	292	464	2.56e-22	93.2
MS.gene81575.t1	MTR_0182s0060	35.838	173	105	3	2	170	346	516	7.04e-22	92.0
MS.gene81575.t1	MTR_3g079390	30.409	171	116	3	2	170	280	449	2.39e-21	90.1
MS.gene81575.t1	MTR_7g060115	34.254	181	105	3	2	178	285	455	2.91e-21	90.1
MS.gene81575.t1	MTR_8g042070	31.771	192	105	5	2	171	77	264	3.45e-20	85.1
MS.gene81575.t1	MTR_8g005225	26.804	194	120	3	3	176	271	462	6.15e-20	86.3
MS.gene81575.t1	MTR_6g009600	39.623	106	62	2	2	106	27	131	2.06e-19	80.5
MS.gene81575.t1	MTR_2g036320	33.908	174	105	6	2	167	290	461	2.06e-19	85.1
MS.gene81575.t1	MTR_7g060160	32.044	181	109	4	2	178	237	407	3.00e-19	84.0
MS.gene81575.t1	MTR_7g446270	34.706	170	106	2	1	166	312	480	4.15e-19	84.0
MS.gene81575.t1	MTR_7g059510	33.143	175	102	4	1	170	253	417	2.03e-18	82.0
MS.gene81575.t1	MTR_7g060370	36.207	174	103	4	2	170	300	470	4.69e-18	80.9
MS.gene81575.t1	MTR_8g005265	28.125	192	116	3	2	173	266	455	9.51e-18	80.1
MS.gene81575.t1	MTR_2g041180	32.584	178	102	8	2	167	274	445	2.40e-16	76.3
MS.gene81575.t1	MTR_8g005325	29.348	184	104	4	2	163	284	463	2.61e-16	75.9
MS.gene81575.t1	MTR_7g060220	34.104	173	108	3	2	170	266	436	4.71e-16	75.1
MS.gene81575.t1	MTR_8g005235	26.562	192	115	4	2	171	254	441	8.57e-15	71.6
MS.gene81575.t1	MTR_7g059810	34.104	173	108	3	2	170	274	444	9.50e-15	71.6
MS.gene81575.t1	MTR_2g036310	31.447	159	99	6	20	170	2	158	2.25e-14	67.4
MS.gene81575.t1	MTR_0209s0060	29.609	179	98	6	2	161	257	426	9.13e-14	68.6
MS.gene81575.t1	MTR_0209s0030	29.609	179	98	6	2	161	257	426	9.13e-14	68.6
MS.gene81575.t1	MTR_4g009590	28.729	181	112	7	6	176	386	559	1.83e-13	67.8
MS.gene81575.t1	MTR_8g005295	27.072	181	106	4	2	158	319	497	4.25e-13	66.6
MS.gene81575.t1	MTR_3g107600	26.923	156	86	4	2	156	443	571	2.47e-11	61.6
MS.gene81575.t1	MTR_4g009620	27.419	186	116	8	2	176	380	557	2.90e-11	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81575.t1	AT3G50120	42.857	175	98	2	2	175	355	528	7.35e-41	144
MS.gene81575.t1	AT3G50160	39.429	175	104	2	2	175	325	498	4.76e-38	136
MS.gene81575.t1	AT3G50170	39.429	175	104	2	2	175	363	536	2.26e-37	135
MS.gene81575.t1	AT3G50150	38.857	175	105	2	2	175	333	506	2.53e-37	134
MS.gene81575.t1	AT3G50140	38.857	175	105	2	2	175	363	536	3.73e-37	134
MS.gene81575.t1	AT4G31980	37.647	170	103	2	2	170	510	677	4.02e-32	121
MS.gene81575.t1	AT3G50130	38.506	174	105	2	2	174	388	560	1.49e-30	116
MS.gene81575.t1	AT3G50180	34.270	178	111	2	2	174	408	584	1.37e-27	108
MS.gene81575.t1	AT3G50190	31.461	178	117	3	2	175	314	490	2.47e-26	104
MS.gene81575.t1	AT5G11290	32.353	170	111	3	2	170	184	350	4.84e-26	102
MS.gene81575.t1	AT5G11290	32.353	170	111	3	2	170	253	419	9.33e-26	102
MS.gene81575.t1	AT3G60470	35.795	176	110	3	2	176	367	540	1.07e-23	97.4
MS.gene81575.t1	AT2G44930	31.319	182	113	4	2	177	327	502	2.11e-21	90.5
MS.gene81575.t1	AT3G47250	30.682	176	118	4	3	175	303	477	1.11e-20	88.6
MS.gene81575.t1	AT3G47250	30.682	176	118	4	3	175	303	477	1.11e-20	88.6
MS.gene81575.t1	AT3G47250	30.682	176	118	4	3	175	303	477	1.11e-20	88.6
MS.gene81575.t1	AT5G22540	33.333	144	93	3	7	148	266	408	2.46e-20	87.4
MS.gene81575.t1	AT2G36430	33.898	177	114	3	2	176	273	448	1.86e-19	85.1
MS.gene81575.t1	AT2G28580	31.410	156	97	3	21	172	382	531	4.14e-19	84.3
MS.gene81575.t1	AT2G28580	31.410	156	97	3	21	172	351	500	4.39e-19	84.0
MS.gene81575.t1	AT2G28580	31.410	156	97	3	21	172	368	517	4.51e-19	84.0
MS.gene81575.t1	AT2G28580	31.410	156	97	3	21	172	365	514	4.76e-19	84.0
MS.gene81575.t1	AT2G28580	31.638	177	109	5	2	172	279	449	5.56e-19	83.6
MS.gene81575.t1	AT5G22550	28.977	176	122	3	2	175	313	487	8.09e-19	83.2
MS.gene81575.t1	AT5G22550	28.977	176	122	3	2	175	264	438	8.66e-19	83.2
MS.gene81575.t1	AT3G44710	28.977	176	123	2	2	175	326	501	6.83e-18	80.5
MS.gene81575.t1	AT5G22560	28.994	169	117	3	2	168	340	507	6.35e-17	77.8
MS.gene81575.t1	AT3G47200	26.163	172	122	4	3	170	285	455	1.26e-15	73.9
MS.gene81575.t1	AT3G47200	26.163	172	122	4	3	170	285	455	1.26e-15	73.9
MS.gene81575.t1	AT1G67150	27.647	170	122	1	3	172	193	361	7.93e-13	65.9
MS.gene81575.t1	AT1G67150	27.647	170	122	1	3	172	240	408	9.32e-13	65.9
MS.gene81575.t1	AT1G67150	27.647	170	122	1	3	172	236	404	1.02e-12	65.5
MS.gene81575.t1	AT1G67150	27.647	170	122	1	3	172	236	404	1.02e-12	65.5
MS.gene81575.t1	AT3G02645	28.000	150	105	2	2	149	350	498	1.61e-11	62.4

Find 35 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCCAACGAGTTGAACAATT+TGG	0.245536	5.1:-68267567	None:intergenic
AAAAGATGTTACCTTGTTAT+CGG	0.302978	5.1:-68267444	None:intergenic
CCTTCAATCGAGGTGTTCTT+AGG	0.321521	5.1:+68267339	MS.gene81575:CDS
CGTTCTCAAGATAGTTGATT+AGG	0.331878	5.1:-68267532	None:intergenic
ATTCCTTAACCTCATGGCTT+TGG	0.361611	5.1:+68267400	MS.gene81575:CDS
GAATAATAGTAAGAGCAAAA+AGG	0.409383	5.1:-68267745	None:intergenic
GCTCCAAAGCCATGAGGTTA+AGG	0.418526	5.1:-68267403	None:intergenic
ATGGATTGCAATGGAGAACT+TGG	0.448261	5.1:+68267602	MS.gene81575:CDS
ACACTTACTTTAAGAATCCA+TGG	0.451026	5.1:+68267693	MS.gene81575:CDS
CCTAAGAACACCTCGATTGA+AGG	0.471335	5.1:-68267339	None:intergenic
AAGTTCTCCATTGCAATCCA+TGG	0.472099	5.1:-68267600	None:intergenic
TGTTATCGGCTTCAACTACA+TGG	0.480168	5.1:-68267430	None:intergenic
GCAATGGAGCTTAAAGAGGC+TGG	0.505520	5.1:+68267272	None:intergenic
ATACATATTCCTTAACCTCA+TGG	0.515139	5.1:+68267394	MS.gene81575:CDS
AGCCAAATTGTTCAACTCGT+TGG	0.520680	5.1:+68267565	MS.gene81575:CDS
AGTTCTCCATTGCAATCCAT+GGG	0.524419	5.1:-68267599	None:intergenic
CACTTACTTTAAGAATCCAT+GGG	0.526297	5.1:+68267694	MS.gene81575:CDS
GTAGGAAACCGTGGAAAAGA+TGG	0.537234	5.1:+68267654	MS.gene81575:CDS
ATCTGCAATGGAGCTTAAAG+AGG	0.545147	5.1:+68267268	None:intergenic
ATTCAAACTATATACACAGT+TGG	0.561149	5.1:+68267764	MS.gene81575:CDS
CAATGGAGAACTTGGAGAAG+TGG	0.569190	5.1:+68267610	MS.gene81575:CDS
AGAGTGTGATTTCTTACTGT+AGG	0.595533	5.1:+68267636	MS.gene81575:CDS
TGTAGTTGAAGCCGATAACA+AGG	0.611122	5.1:+68267433	MS.gene81575:CDS
TGTCTACTACTATTAAGAGC+GGG	0.613862	5.1:-68267364	None:intergenic
ACAAGAGATACAATAGCCCA+TGG	0.623258	5.1:-68267710	None:intergenic
TTTCTTACTGTAGGAAACCG+TGG	0.625114	5.1:+68267645	MS.gene81575:CDS
AGTTGATTAGGATATGACTT+CGG	0.631023	5.1:-68267520	None:intergenic
AGAGATGTTTCCTTCAATCG+AGG	0.652766	5.1:+68267329	MS.gene81575:CDS
TTCTCCATTGCAATCCATGG+GGG	0.668558	5.1:-68267597	None:intergenic
GCTTCAAGAAAAGCGAAACG+AGG	0.673060	5.1:+68267300	MS.gene81575:CDS
CTTAATAGTAGTAGACAACA+CGG	0.677320	5.1:+68267370	MS.gene81575:CDS
TTGTCTACTACTATTAAGAG+CGG	0.691634	5.1:-68267365	None:intergenic
GTTCTCCATTGCAATCCATG+GGG	0.702774	5.1:-68267598	None:intergenic
GATTGAAGGAAACATCTCTG+AGG	0.729285	5.1:-68267325	None:intergenic
GGAGACGCTCCAAAGCCATG+AGG	0.747403	5.1:-68267409	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATTCAAACTATATACACAGT+TGG	+	chr5.1:68267764-68267783	MS.gene81575:CDS	25.0%
!	GAATAATAGTAAGAGCAAAA+AGG	-	chr5.1:68267748-68267767	None:intergenic	25.0%
!!	AAAAGATGTTACCTTGTTAT+CGG	-	chr5.1:68267447-68267466	None:intergenic	25.0%
!!	AACATCTTTTGTATGCTTTA+TGG	+	chr5.1:68267457-68267476	MS.gene81575:CDS	25.0%
!!	AGTTGGTCAATTTTATCAAA+AGG	+	chr5.1:68267781-68267800	MS.gene81575:CDS	25.0%
	AGTTGATTAGGATATGACTT+CGG	-	chr5.1:68267523-68267542	None:intergenic	30.0%
	ATACATATTCCTTAACCTCA+TGG	+	chr5.1:68267394-68267413	MS.gene81575:CDS	30.0%
	CTTAATAGTAGTAGACAACA+CGG	+	chr5.1:68267370-68267389	MS.gene81575:CDS	30.0%
	TTGTCTACTACTATTAAGAG+CGG	-	chr5.1:68267368-68267387	None:intergenic	30.0%
!	ACACTTACTTTAAGAATCCA+TGG	+	chr5.1:68267693-68267712	MS.gene81575:CDS	30.0%
!	CACTTACTTTAAGAATCCAT+GGG	+	chr5.1:68267694-68267713	MS.gene81575:CDS	30.0%
!	GGTCAATTTTATCAAAAGGA+GGG	+	chr5.1:68267785-68267804	MS.gene81575:CDS	30.0%
!	TGGTCAATTTTATCAAAAGG+AGG	+	chr5.1:68267784-68267803	MS.gene81575:CDS	30.0%
	AGAGTGTGATTTCTTACTGT+AGG	+	chr5.1:68267636-68267655	MS.gene81575:CDS	35.0%
	CGTTCTCAAGATAGTTGATT+AGG	-	chr5.1:68267535-68267554	None:intergenic	35.0%
	TGTCTACTACTATTAAGAGC+GGG	-	chr5.1:68267367-68267386	None:intergenic	35.0%
	AAGAGCAAAAAGGAAGACAG+CGG	-	chr5.1:68267738-68267757	None:intergenic	40.0%
	AAGTTCTCCATTGCAATCCA+TGG	-	chr5.1:68267603-68267622	None:intergenic	40.0%
	ACAAGAGATACAATAGCCCA+TGG	-	chr5.1:68267713-68267732	None:intergenic	40.0%
	AGAGATGTTTCCTTCAATCG+AGG	+	chr5.1:68267329-68267348	MS.gene81575:CDS	40.0%
	AGCCAAATTGTTCAACTCGT+TGG	+	chr5.1:68267565-68267584	MS.gene81575:CDS	40.0%
	AGTTCTCCATTGCAATCCAT+GGG	-	chr5.1:68267602-68267621	None:intergenic	40.0%
	ATTCCTTAACCTCATGGCTT+TGG	+	chr5.1:68267400-68267419	MS.gene81575:CDS	40.0%
	GATTGAAGGAAACATCTCTG+AGG	-	chr5.1:68267328-68267347	None:intergenic	40.0%
	TGTAGTTGAAGCCGATAACA+AGG	+	chr5.1:68267433-68267452	MS.gene81575:CDS	40.0%
	TTTCTTACTGTAGGAAACCG+TGG	+	chr5.1:68267645-68267664	MS.gene81575:CDS	40.0%
!	ATGGATTGCAATGGAGAACT+TGG	+	chr5.1:68267602-68267621	MS.gene81575:CDS	40.0%
!	TGCCAACGAGTTGAACAATT+TGG	-	chr5.1:68267570-68267589	None:intergenic	40.0%
!!	TGTTATCGGCTTCAACTACA+TGG	-	chr5.1:68267433-68267452	None:intergenic	40.0%
	CAATGGAGAACTTGGAGAAG+TGG	+	chr5.1:68267610-68267629	MS.gene81575:CDS	45.0%
	CCTAAGAACACCTCGATTGA+AGG	-	chr5.1:68267342-68267361	None:intergenic	45.0%
	GCTTCAAGAAAAGCGAAACG+AGG	+	chr5.1:68267300-68267319	MS.gene81575:CDS	45.0%
	GTAGGAAACCGTGGAAAAGA+TGG	+	chr5.1:68267654-68267673	MS.gene81575:CDS	45.0%
	GTTCTCCATTGCAATCCATG+GGG	-	chr5.1:68267601-68267620	None:intergenic	45.0%
	TTCTCCATTGCAATCCATGG+GGG	-	chr5.1:68267600-68267619	None:intergenic	45.0%
!	CCTTCAATCGAGGTGTTCTT+AGG	+	chr5.1:68267339-68267358	MS.gene81575:CDS	45.0%
	GCTCCAAAGCCATGAGGTTA+AGG	-	chr5.1:68267406-68267425	None:intergenic	50.0%
!	CTTGCACGCCATCTTTTCCA+CGG	-	chr5.1:68267665-68267684	None:intergenic	50.0%
	GTTGGCAAAAGAAGCCCCCA+TGG	+	chr5.1:68267583-68267602	MS.gene81575:CDS	55.0%
!	GAAGCCCCCATGGATTGCAA+TGG	+	chr5.1:68267593-68267612	MS.gene81575:CDS	55.0%
	GGAGACGCTCCAAAGCCATG+AGG	-	chr5.1:68267412-68267431	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	68267275	68267811	68267275	ID=MS.gene81575
chr5.1	mRNA	68267275	68267811	68267275	ID=MS.gene81575.t1;Parent=MS.gene81575
chr5.1	exon	68267275	68267811	68267275	ID=MS.gene81575.t1.exon1;Parent=MS.gene81575.t1
chr5.1	CDS	68267275	68267811	68267275	ID=cds.MS.gene81575.t1;Parent=MS.gene81575.t1

Gene Sequence

>MS.gene81575

ATGGAGCTTAAAGAGGCTGGAATCAGCTTCAAGAAAAGCGAAACGAGGAGCCTCAGAGATGTTTCCTTCAATCGAGGTGTTCTTAGGCTCCCGCTCTTAATAGTAGTAGACAACACGGAATACATATTCCTTAACCTCATGGCTTTGGAGCGTCTCCATGTAGTTGAAGCCGATAACAAGGTAACATCTTTTGTATGCTTTATGGACACTATCATCGACAGTACTAAAGATGTTGAAATTCTCATCCGAAGTCATATCCTAATCAACTATCTTGAGAACGATGAAGCTGTAGCCAAATTGTTCAACTCGTTGGCAAAAGAAGCCCCCATGGATTGCAATGGAGAACTTGGAGAAGTGGCGAAGAGTGTGATTTCTTACTGTAGGAAACCGTGGAAAAGATGGCGTGCAAGTCTCATTCACACTTACTTTAAGAATCCATGGGCTATTGTATCTCTTGTCGCCGCTGTCTTCCTTTTTGCTCTTACTATTATTCAAACTATATACACAGTTGGTCAATTTTATCAAAAGGAGGGCTAG

Protein sequence

>MS.gene81575.t1

MELKEAGISFKKSETRSLRDVSFNRGVLRLPLLIVVDNTEYIFLNLMALERLHVVEADNKVTSFVCFMDTIIDSTKDVEILIRSHILINYLENDEAVAKLFNSLAKEAPMDCNGELGEVAKSVISYCRKPWKRWRASLIHTYFKNPWAIVSLVAAVFLFALTIIQTIYTVGQFYQKEG