Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81642.t1 | XP_003616876.2 | 99.9 | 677 | 1 | 0 | 1 | 677 | 1 | 677 | 0.00E+00 | 1363.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81642.t1 | P93471 | 94.1 | 677 | 35 | 1 | 1 | 677 | 1 | 672 | 0.0e+00 | 1276.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81642.t1 | G7KH91 | 99.9 | 677 | 1 | 0 | 1 | 677 | 1 | 677 | 0.0e+00 | 1363.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049369 | MS.gene81642 | 0.804602 | 2.00E-49 | -1.69E-46 |
| MS.gene057668 | MS.gene81642 | 0.855128 | 7.76E-62 | -1.69E-46 |
| MS.gene058075 | MS.gene81642 | 0.803133 | 4.03E-49 | -1.69E-46 |
| MS.gene059674 | MS.gene81642 | 0.809828 | 1.56E-50 | -1.69E-46 |
| MS.gene059807 | MS.gene81642 | 0.836476 | 9.15E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81642.t1 | MTR_5g085250 | 99.852 | 677 | 1 | 0 | 1 | 677 | 1 | 677 | 0.0 | 1417 |
| MS.gene81642.t1 | MTR_5g085250 | 100.000 | 545 | 0 | 0 | 133 | 677 | 29 | 573 | 0.0 | 1135 |
| MS.gene81642.t1 | MTR_5g085250 | 100.000 | 540 | 0 | 0 | 138 | 677 | 1 | 540 | 0.0 | 1125 |
| MS.gene81642.t1 | MTR_2g085210 | 49.138 | 348 | 170 | 2 | 335 | 675 | 527 | 874 | 6.59e-114 | 363 |
| MS.gene81642.t1 | MTR_5g009530 | 47.688 | 346 | 169 | 3 | 341 | 675 | 689 | 1033 | 2.24e-106 | 347 |
| MS.gene81642.t1 | MTR_8g027985 | 45.170 | 352 | 180 | 3 | 336 | 675 | 613 | 963 | 3.30e-102 | 334 |
| MS.gene81642.t1 | MTR_5g009530 | 48.951 | 286 | 138 | 1 | 341 | 618 | 689 | 974 | 1.43e-90 | 303 |
| MS.gene81642.t1 | MTR_2g085210 | 50.307 | 163 | 81 | 0 | 335 | 497 | 527 | 689 | 2.17e-48 | 181 |
| MS.gene81642.t1 | MTR_2g085210 | 50.307 | 163 | 81 | 0 | 335 | 497 | 527 | 689 | 2.50e-48 | 181 |
| MS.gene81642.t1 | MTR_5g009530 | 46.281 | 121 | 64 | 1 | 341 | 461 | 689 | 808 | 9.47e-29 | 123 |
| MS.gene81642.t1 | MTR_5g016370 | 21.277 | 329 | 221 | 10 | 356 | 673 | 290 | 591 | 9.72e-13 | 71.6 |
| MS.gene81642.t1 | MTR_1g072140 | 30.058 | 173 | 111 | 6 | 427 | 593 | 116 | 284 | 1.43e-12 | 69.7 |
| MS.gene81642.t1 | MTR_6g004040 | 26.738 | 187 | 129 | 6 | 452 | 634 | 1 | 183 | 1.57e-12 | 71.2 |
| MS.gene81642.t1 | MTR_7g086360 | 26.042 | 192 | 131 | 6 | 437 | 619 | 112 | 301 | 1.29e-11 | 67.0 |
| MS.gene81642.t1 | MTR_4g073080 | 29.268 | 164 | 105 | 4 | 428 | 581 | 168 | 330 | 1.65e-11 | 67.0 |
| MS.gene81642.t1 | MTR_4g095058 | 22.086 | 326 | 209 | 11 | 364 | 673 | 278 | 574 | 6.92e-11 | 65.9 |
| MS.gene81642.t1 | MTR_4g095058 | 22.086 | 326 | 209 | 11 | 364 | 673 | 276 | 572 | 7.99e-11 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81642.t1 | AT2G32950 | 77.647 | 680 | 144 | 6 | 1 | 677 | 1 | 675 | 0.0 | 1096 |
| MS.gene81642.t1 | AT2G32950 | 80.839 | 548 | 99 | 4 | 132 | 677 | 19 | 562 | 0.0 | 922 |
| MS.gene81642.t1 | AT3G15354 | 36.508 | 567 | 290 | 9 | 148 | 673 | 54 | 591 | 4.14e-115 | 358 |
| MS.gene81642.t1 | AT3G15354 | 36.508 | 567 | 290 | 9 | 148 | 673 | 54 | 591 | 4.14e-115 | 358 |
| MS.gene81642.t1 | AT1G53090 | 46.849 | 365 | 185 | 3 | 316 | 673 | 208 | 570 | 9.48e-115 | 356 |
| MS.gene81642.t1 | AT3G15354 | 36.508 | 567 | 290 | 9 | 148 | 673 | 304 | 841 | 6.35e-112 | 357 |
| MS.gene81642.t1 | AT1G53090 | 46.849 | 365 | 185 | 3 | 316 | 673 | 429 | 791 | 6.38e-112 | 355 |
| MS.gene81642.t1 | AT1G53090 | 46.849 | 365 | 185 | 3 | 316 | 673 | 429 | 791 | 6.38e-112 | 355 |
| MS.gene81642.t1 | AT1G53090 | 46.849 | 365 | 185 | 3 | 316 | 673 | 429 | 791 | 6.38e-112 | 355 |
| MS.gene81642.t1 | AT4G11110 | 48.071 | 337 | 165 | 2 | 349 | 675 | 699 | 1035 | 4.52e-105 | 343 |
| MS.gene81642.t1 | AT3G15354 | 35.626 | 567 | 287 | 10 | 148 | 673 | 304 | 833 | 1.16e-104 | 337 |
| MS.gene81642.t1 | AT2G46340 | 37.381 | 527 | 280 | 9 | 160 | 675 | 541 | 1028 | 3.51e-104 | 340 |
| MS.gene81642.t1 | AT2G46340 | 37.381 | 527 | 280 | 9 | 160 | 675 | 541 | 1028 | 3.51e-104 | 340 |
| MS.gene81642.t1 | AT5G23730 | 35.061 | 328 | 181 | 11 | 374 | 674 | 41 | 363 | 3.06e-57 | 198 |
| MS.gene81642.t1 | AT5G52250 | 33.746 | 323 | 181 | 12 | 372 | 673 | 70 | 380 | 6.18e-49 | 176 |
| MS.gene81642.t1 | AT1G53090 | 45.604 | 182 | 97 | 1 | 316 | 497 | 429 | 608 | 2.70e-48 | 180 |
| MS.gene81642.t1 | AT3G15354 | 30.729 | 384 | 203 | 7 | 148 | 497 | 304 | 658 | 1.02e-47 | 179 |
| MS.gene81642.t1 | AT3G15610 | 26.906 | 223 | 146 | 7 | 367 | 585 | 93 | 302 | 1.45e-13 | 73.2 |
| MS.gene81642.t1 | AT5G08390 | 27.933 | 179 | 123 | 5 | 458 | 634 | 9 | 183 | 4.16e-13 | 73.2 |
| MS.gene81642.t1 | AT4G35050 | 24.242 | 198 | 137 | 6 | 396 | 581 | 141 | 337 | 7.02e-13 | 71.6 |
| MS.gene81642.t1 | AT4G18900 | 27.488 | 211 | 140 | 6 | 421 | 619 | 233 | 442 | 1.34e-12 | 70.9 |
| MS.gene81642.t1 | AT5G23430 | 27.072 | 181 | 126 | 5 | 452 | 628 | 1 | 179 | 3.10e-12 | 70.5 |
| MS.gene81642.t1 | AT5G23430 | 27.072 | 181 | 126 | 5 | 452 | 628 | 1 | 179 | 3.10e-12 | 70.5 |
| MS.gene81642.t1 | AT5G23430 | 27.072 | 181 | 126 | 5 | 452 | 628 | 1 | 179 | 3.16e-12 | 70.5 |
| MS.gene81642.t1 | AT2G43770 | 25.907 | 193 | 132 | 6 | 436 | 619 | 109 | 299 | 3.35e-12 | 68.9 |
| MS.gene81642.t1 | AT5G58230 | 25.833 | 240 | 154 | 9 | 357 | 579 | 115 | 347 | 4.75e-12 | 68.9 |
| MS.gene81642.t1 | AT3G49660 | 25.743 | 202 | 140 | 7 | 427 | 622 | 118 | 315 | 2.24e-11 | 66.2 |
| MS.gene81642.t1 | AT4G35370 | 28.643 | 199 | 134 | 5 | 428 | 618 | 222 | 420 | 2.97e-11 | 66.6 |
| MS.gene81642.t1 | AT1G52730 | 25.225 | 222 | 152 | 6 | 367 | 584 | 93 | 304 | 3.29e-11 | 65.9 |
| MS.gene81642.t1 | AT1G52730 | 25.225 | 222 | 152 | 6 | 367 | 584 | 93 | 304 | 3.29e-11 | 65.9 |
| MS.gene81642.t1 | AT2G16780 | 23.737 | 198 | 138 | 6 | 396 | 581 | 140 | 336 | 5.99e-11 | 65.5 |
Find 148 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCCTTTGGCAGAGTTTAA+TGG | 0.237138 | 5.1:+67098670 | MS.gene81642:intron |
| CAGATAGTACACTGCGGTTA+TGG | 0.252524 | 5.1:+67102371 | MS.gene81642:CDS |
| AACCCTCCTCGAATATTTAA+AGG | 0.258388 | 5.1:-67093977 | None:intergenic |
| ATTCGAGGAGGGTTGTCTTC+AGG | 0.287880 | 5.1:+67093986 | MS.gene81642:CDS |
| TTTGTAGATAACATTCTTGT+AGG | 0.307788 | 5.1:-67095184 | None:intergenic |
| TTGCGGATGCTTGATGATTC+TGG | 0.320860 | 5.1:+67093896 | MS.gene81642:CDS |
| TATTTCTCAAATCATAATAA+TGG | 0.348001 | 5.1:-67102258 | None:intergenic |
| ACTTCATCAGTGCTGTTTGC+TGG | 0.354397 | 5.1:+67103111 | MS.gene81642:CDS |
| TCCGAATTTCTTACTCGATA+AGG | 0.355252 | 5.1:+67089894 | MS.gene81642:CDS |
| CACTGATGGGATCTCTTCTC+TGG | 0.356789 | 5.1:-67094066 | None:intergenic |
| ATGAAGGAATTGTGACTGTT+TGG | 0.358639 | 5.1:+67098503 | MS.gene81642:CDS |
| ATTCGGAAACAAATTACTAT+TGG | 0.363357 | 5.1:-67089878 | None:intergenic |
| CAAAGTTTGGTGCACGAATC+AGG | 0.364240 | 5.1:+67101665 | MS.gene81642:CDS |
| TTGACTATTTGCAGTTAGTA+TGG | 0.366718 | 5.1:-67103150 | None:intergenic |
| AATTCGTATCTCTTTCTTGT+TGG | 0.388471 | 5.1:-67095238 | None:intergenic |
| TTCACAGTATATAAATCAAT+CGG | 0.394235 | 5.1:+67094096 | MS.gene81642:CDS |
| AGCAAGCACCAGCACTTTGA+TGG | 0.401646 | 5.1:-67103177 | None:intergenic |
| TTCGAGGAGGGTTGTCTTCA+GGG | 0.403288 | 5.1:+67093987 | MS.gene81642:CDS |
| AGATAGTACACTGCGGTTAT+GGG | 0.404349 | 5.1:+67102372 | MS.gene81642:CDS |
| CAATGTCCTCCTTTATGAAC+TGG | 0.413835 | 5.1:-67093814 | None:intergenic |
| TCTTTGAGCAGGTCAAAGTT+TGG | 0.423567 | 5.1:+67101652 | MS.gene81642:intron |
| TGAAGGAATTGTGACTGTTT+GGG | 0.426678 | 5.1:+67098504 | MS.gene81642:CDS |
| AGTTCATAAAGGAGGACATT+GGG | 0.430648 | 5.1:+67093816 | MS.gene81642:CDS |
| ATTCAGCCAACATCGTTTCA+AGG | 0.444771 | 5.1:+67096355 | MS.gene81642:CDS |
| CTATTTGCAGTTAGTATGGT+GGG | 0.447922 | 5.1:-67103146 | None:intergenic |
| GACGATTTGTTTGCTACAGC+TGG | 0.448381 | 5.1:+67097109 | MS.gene81642:CDS |
| TGTGTATTATTACTCATCTT+CGG | 0.448436 | 5.1:+67089818 | MS.gene81642:CDS |
| CAAAACTTAGTTGCTTGAGT+TGG | 0.449682 | 5.1:+67098443 | MS.gene81642:CDS |
| GTGAAGTATAATCCTGGATC+GGG | 0.450446 | 5.1:+67101732 | MS.gene81642:CDS |
| TCCTCCGCATCATCCATGTC+AGG | 0.456144 | 5.1:-67103074 | None:intergenic |
| TCCGGTGACGGTTACGGTGG+TGG | 0.456824 | 5.1:-67089665 | None:intergenic |
| GACGCGTTTCTTACAGCTTG+TGG | 0.459464 | 5.1:+67089778 | MS.gene81642:CDS |
| GGTTCGGGTTTGACGACGGC+GGG | 0.460222 | 5.1:-67089628 | None:intergenic |
| AACTGATCTCCAGTTCATAA+AGG | 0.467802 | 5.1:+67093805 | MS.gene81642:CDS |
| TTGATAATCTGCATACAAAT+CGG | 0.468114 | 5.1:-67089754 | None:intergenic |
| ACCCTCCTCGAATATTTAAA+GGG | 0.470850 | 5.1:-67093976 | None:intergenic |
| AAAGGAGGACATTGGGGCCG+TGG | 0.471087 | 5.1:+67093823 | MS.gene81642:CDS |
| AAGTCTCAAATCAGCTCTCA+TGG | 0.472937 | 5.1:+67094034 | MS.gene81642:CDS |
| AAAATCATGGCATTCATCAA+TGG | 0.479333 | 5.1:+67093925 | MS.gene81642:CDS |
| TCTCAGCAAGGAGTGACAAA+AGG | 0.479384 | 5.1:-67093344 | None:intergenic |
| TGACAACTTTCACACGCTAC+AGG | 0.480406 | 5.1:+67095312 | MS.gene81642:CDS |
| GCAATGTAATTCCCCGATCC+AGG | 0.483126 | 5.1:-67101744 | None:intergenic |
| ACCTTATCGAGTAAGAAATT+CGG | 0.487068 | 5.1:-67089895 | None:intergenic |
| CGTGTGGTCATCTCCACAAC+AGG | 0.491234 | 5.1:-67098418 | None:intergenic |
| TCTTGTGCCCACTGAAAACA+TGG | 0.491272 | 5.1:-67102294 | None:intergenic |
| TGTATTCACTACTTACTGTA+AGG | 0.494510 | 5.1:-67102888 | None:intergenic |
| TGCCCACTGAAAACATGGAC+TGG | 0.495346 | 5.1:-67102289 | None:intergenic |
| GAAACAAATTACTATTGGTA+AGG | 0.495996 | 5.1:-67089873 | None:intergenic |
| GCCTGCCGGAAATGCTCCAC+GGG | 0.497681 | 5.1:-67090881 | None:intergenic |
| GATCCCTCTATGCTTGTATC+CGG | 0.500871 | 5.1:+67098743 | MS.gene81642:CDS |
| GTCACCGCCGGCGATGTCTC+CGG | 0.501444 | 5.1:-67089683 | None:intergenic |
| ATCCGGTAGTGATGATTGTA+AGG | 0.506760 | 5.1:+67098760 | MS.gene81642:CDS |
| GATGTCTCCGGTGACGGTTA+CGG | 0.509729 | 5.1:-67089671 | None:intergenic |
| ACTTATATCGTGCTCGGGAT+CGG | 0.512625 | 5.1:+67093861 | MS.gene81642:CDS |
| TAGATTTAGTTCCCCTGACA+TGG | 0.513170 | 5.1:+67103061 | MS.gene81642:CDS |
| CAAATCAGCTCTCATGGACA+TGG | 0.515444 | 5.1:+67094040 | MS.gene81642:CDS |
| TTTCCGGCAGGCGATACAAA+AGG | 0.515478 | 5.1:+67090892 | MS.gene81642:CDS |
| CTCAGCAAGGAGTGACAAAA+GGG | 0.516560 | 5.1:-67093343 | None:intergenic |
| TCCACCACCGTAACCGTCAC+CGG | 0.517126 | 5.1:+67089664 | MS.gene81642:CDS |
| GAATGGACTTATATCGTGCT+CGG | 0.518337 | 5.1:+67093855 | MS.gene81642:CDS |
| CCCCGTGGAGCATTTCCGGC+AGG | 0.521449 | 5.1:+67090880 | MS.gene81642:CDS |
| ACTATTTGCAGTTAGTATGG+TGG | 0.523504 | 5.1:-67103147 | None:intergenic |
| CGCCTGCCGGAAATGCTCCA+CGG | 0.524040 | 5.1:-67090882 | None:intergenic |
| CTGTGAATCTGATCCACTGA+TGG | 0.527140 | 5.1:-67094080 | None:intergenic |
| AAATCTCCAAGCCTCTGACT+TGG | 0.530640 | 5.1:+67103036 | MS.gene81642:CDS |
| AATCATAATAATGGATGTGA+TGG | 0.531014 | 5.1:-67102249 | None:intergenic |
| CATGTCTATATTTAAAACAC+TGG | 0.531620 | 5.1:-67101691 | None:intergenic |
| TCTAGCCCTTTAAATATTCG+AGG | 0.534972 | 5.1:+67093971 | MS.gene81642:CDS |
| CATGGATGATGCGGAGGATG+AGG | 0.535745 | 5.1:+67103079 | MS.gene81642:CDS |
| GATGATGCGGAGGATGAGGC+TGG | 0.535882 | 5.1:+67103083 | MS.gene81642:CDS |
| TACCTTACAATCATCACTAC+CGG | 0.541446 | 5.1:-67098762 | None:intergenic |
| TGCTGATGCAAGTTCATCGT+TGG | 0.542829 | 5.1:-67102344 | None:intergenic |
| GCCGGCGATGTCTCCGGTGA+CGG | 0.545405 | 5.1:-67089677 | None:intergenic |
| ACTCCGTCATAGTTGCGATC+GGG | 0.545526 | 5.1:-67089711 | None:intergenic |
| AATGGACTTATATCGTGCTC+GGG | 0.547396 | 5.1:+67093856 | MS.gene81642:CDS |
| TCACAGTATATAAATCAATC+GGG | 0.550556 | 5.1:+67094097 | MS.gene81642:CDS |
| TCAAGGCACCATCAAAGTGC+TGG | 0.553897 | 5.1:+67103169 | MS.gene81642:CDS |
| TGGTTCGGGTTTGACGACGG+CGG | 0.555209 | 5.1:-67089629 | None:intergenic |
| TCCGACGGAGTGTTCTTCCA+TGG | 0.556975 | 5.1:-67089596 | None:intergenic |
| GCCCTTTAAATATTCGAGGA+GGG | 0.557638 | 5.1:+67093975 | MS.gene81642:CDS |
| TGTGAAGTATAATCCTGGAT+CGG | 0.558008 | 5.1:+67101731 | MS.gene81642:CDS |
| AACTCCGTCATAGTTGCGAT+CGG | 0.558278 | 5.1:-67089712 | None:intergenic |
| TCTATGCCAAGTCAGAGGCT+TGG | 0.560666 | 5.1:-67103042 | None:intergenic |
| ATCGGTACTCTGTGAAGTTG+CGG | 0.565158 | 5.1:+67093879 | MS.gene81642:CDS |
| AACACACCTTGAAACGATGT+TGG | 0.565732 | 5.1:-67096361 | None:intergenic |
| TGTTGTGTGAAGTATAATCC+TGG | 0.566028 | 5.1:+67101726 | MS.gene81642:CDS |
| AACAGGACAATGAGCATCTG+TGG | 0.567948 | 5.1:-67098401 | None:intergenic |
| GGATGGTTCGGGTTTGACGA+CGG | 0.571721 | 5.1:-67089632 | None:intergenic |
| AAGAGCATGAAAAGCGTGCA+TGG | 0.573190 | 5.1:+67098699 | MS.gene81642:CDS |
| CACTGAAAACATGGACTGGA+CGG | 0.574289 | 5.1:-67102285 | None:intergenic |
| GGCCGTGGAGAAACATAGAA+TGG | 0.575022 | 5.1:+67093838 | MS.gene81642:CDS |
| ACGAATTTCATAAGTCGAGA+AGG | 0.577292 | 5.1:+67095254 | MS.gene81642:CDS |
| CCTCCGCATCATCCATGTCA+GGG | 0.577657 | 5.1:-67103073 | None:intergenic |
| TATTTAAAGGGCTAGATGCA+AGG | 0.578156 | 5.1:-67093964 | None:intergenic |
| AAAGAAGAGGGTGCATACAC+AGG | 0.578452 | 5.1:+67094135 | MS.gene81642:CDS |
| TTGCTCTAGTTAGAAAGAAG+AGG | 0.582886 | 5.1:+67094122 | MS.gene81642:CDS |
| TTTCCCGATCGCAACTATGA+CGG | 0.587012 | 5.1:+67089708 | MS.gene81642:CDS |
| ATAGCTAGTAGTGATTATGA+AGG | 0.589727 | 5.1:+67098487 | MS.gene81642:CDS |
| AGATGCTCATTGTCCTGTTG+TGG | 0.590252 | 5.1:+67098405 | MS.gene81642:CDS |
| TTCCCCTGACATGGATGATG+CGG | 0.590370 | 5.1:+67103070 | MS.gene81642:CDS |
| TTGACGACGGCGGGAACGAG+TGG | 0.591311 | 5.1:-67089619 | None:intergenic |
| GTCCATTCTATGTTTCTCCA+CGG | 0.592829 | 5.1:-67093840 | None:intergenic |
| CAGGCTGCAGATAAGCCACA+TGG | 0.596225 | 5.1:+67095215 | MS.gene81642:CDS |
| ACCGTCACCGGAGACATCGC+CGG | 0.598356 | 5.1:+67089676 | MS.gene81642:CDS |
| TCTCTTTCTTGTTGGCCATG+TGG | 0.598443 | 5.1:-67095230 | None:intergenic |
| AGCCCTTTAAATATTCGAGG+AGG | 0.599877 | 5.1:+67093974 | MS.gene81642:CDS |
| GGCGGGAACGAGTGGTCCGA+CGG | 0.601510 | 5.1:-67089611 | None:intergenic |
| CTACCGGATACAAGCATAGA+GGG | 0.604059 | 5.1:-67098746 | None:intergenic |
| TGCTCTAGTTAGAAAGAAGA+GGG | 0.610073 | 5.1:+67094123 | MS.gene81642:CDS |
| TATTTGCAGTTAGTATGGTG+GGG | 0.611419 | 5.1:-67103145 | None:intergenic |
| CCCTGACATGGATGATGCGG+AGG | 0.614289 | 5.1:+67103073 | MS.gene81642:CDS |
| TTGGGATGTGACCACTCGAA+AGG | 0.618691 | 5.1:+67098522 | MS.gene81642:CDS |
| GTACAGGTCCGCACTTTCAG+AGG | 0.618814 | 5.1:+67102838 | MS.gene81642:intron |
| ACTACCGGATACAAGCATAG+AGG | 0.622223 | 5.1:-67098747 | None:intergenic |
| TGACGGTTACGGTGGTGGAG+AGG | 0.622505 | 5.1:-67089660 | None:intergenic |
| ATACTAACTGCAAATAGTCA+AGG | 0.622948 | 5.1:+67103152 | MS.gene81642:CDS |
| ATTCTGGTGGAAGAAAATCA+TGG | 0.624185 | 5.1:+67093912 | MS.gene81642:CDS |
| CTAAATCTATGCCAAGTCAG+AGG | 0.627688 | 5.1:-67103047 | None:intergenic |
| CGGATGCTTGATGATTCTGG+TGG | 0.629544 | 5.1:+67093899 | MS.gene81642:CDS |
| CAGAGAAGAGATCCCATCAG+TGG | 0.630890 | 5.1:+67094067 | MS.gene81642:CDS |
| GTTCACAATTACCTTTCGAG+TGG | 0.633179 | 5.1:-67098533 | None:intergenic |
| TGAAGTATTTGTCTACCACA+AGG | 0.636400 | 5.1:+67102933 | MS.gene81642:CDS |
| GATCGGGAAATTGTCACCGC+CGG | 0.637351 | 5.1:-67089695 | None:intergenic |
| CAGTTCATAAAGGAGGACAT+TGG | 0.639000 | 5.1:+67093815 | MS.gene81642:CDS |
| TGTGAATCTGATCCACTGAT+GGG | 0.640014 | 5.1:-67094079 | None:intergenic |
| TAGCGTGGCCTCTGAAAGTG+CGG | 0.641991 | 5.1:-67102846 | None:intergenic |
| GTCTCCGGTGACGGTTACGG+TGG | 0.644114 | 5.1:-67089668 | None:intergenic |
| TGATCTCCAGTTCATAAAGG+AGG | 0.645464 | 5.1:+67093808 | MS.gene81642:CDS |
| ACAGGACAATGAGCATCTGT+GGG | 0.650389 | 5.1:-67098400 | None:intergenic |
| GTTCATAAAGGAGGACATTG+GGG | 0.653194 | 5.1:+67093817 | MS.gene81642:CDS |
| CTCCGCATCATCCATGTCAG+GGG | 0.653353 | 5.1:-67103072 | None:intergenic |
| TAGCAAACAAATCGTCATCA+CGG | 0.653471 | 5.1:-67097102 | None:intergenic |
| AGTCGAGAAGGTTACAGTGG+TGG | 0.654884 | 5.1:+67095266 | MS.gene81642:CDS |
| AGTAGTGAATACATTGCATG+TGG | 0.658370 | 5.1:+67102898 | MS.gene81642:CDS |
| GGGTTGTGAAGTGACAATGA+AGG | 0.664179 | 5.1:+67093311 | MS.gene81642:intron |
| ACCATGGAAGAACACTCCGT+CGG | 0.669086 | 5.1:+67089595 | None:intergenic |
| TCAATGGACAAGAATAGCAG+TGG | 0.671034 | 5.1:+67093941 | MS.gene81642:CDS |
| GTCATTGCAGAAATAAGACA+CGG | 0.676332 | 5.1:+67096321 | MS.gene81642:CDS |
| AAAAGTGATTGTCCTTGCTG+TGG | 0.679991 | 5.1:+67089844 | MS.gene81642:CDS |
| ATAAGTCGAGAAGGTTACAG+TGG | 0.684944 | 5.1:+67095263 | MS.gene81642:CDS |
| AAAAGTTGATGAGTTGAAGG+AGG | 0.690590 | 5.1:+67093446 | MS.gene81642:CDS |
| GTAAGGTAATGACCACAGCA+AGG | 0.699689 | 5.1:-67089856 | None:intergenic |
| CCTGCCGGAAATGCTCCACG+GGG | 0.700061 | 5.1:-67090880 | None:intergenic |
| TGAAGTATAATCCTGGATCG+GGG | 0.700073 | 5.1:+67101733 | MS.gene81642:CDS |
| GTCACCGGAGACATCGCCGG+CGG | 0.708970 | 5.1:+67089679 | MS.gene81642:CDS |
| AGTTTGGTGCACGAATCAGG+AGG | 0.729323 | 5.1:+67101668 | MS.gene81642:CDS |
| CTGCCGGAAATGCTCCACGG+GGG | 0.738551 | 5.1:-67090879 | None:intergenic |
| CATCAACAGATAGTACACTG+CGG | 0.845506 | 5.1:+67102365 | MS.gene81642:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 67089598 | 67103205 | 67089598 | ID=MS.gene81642 |
| chr5.1 | mRNA | 67089598 | 67103205 | 67089598 | ID=MS.gene81642.t1;Parent=MS.gene81642 |
| chr5.1 | exon | 67089598 | 67089915 | 67089598 | ID=MS.gene81642.t1.exon1;Parent=MS.gene81642.t1 |
| chr5.1 | CDS | 67089598 | 67089915 | 67089598 | ID=cds.MS.gene81642.t1;Parent=MS.gene81642.t1 |
| chr5.1 | exon | 67090836 | 67090913 | 67090836 | ID=MS.gene81642.t1.exon2;Parent=MS.gene81642.t1 |
| chr5.1 | CDS | 67090836 | 67090913 | 67090836 | ID=cds.MS.gene81642.t1;Parent=MS.gene81642.t1 |
| chr5.1 | exon | 67093312 | 67093467 | 67093312 | ID=MS.gene81642.t1.exon3;Parent=MS.gene81642.t1 |
| chr5.1 | CDS | 67093312 | 67093467 | 67093312 | ID=cds.MS.gene81642.t1;Parent=MS.gene81642.t1 |
| chr5.1 | exon | 67093800 | 67094156 | 67093800 | ID=MS.gene81642.t1.exon4;Parent=MS.gene81642.t1 |
| chr5.1 | CDS | 67093800 | 67094156 | 67093800 | ID=cds.MS.gene81642.t1;Parent=MS.gene81642.t1 |
| chr5.1 | exon | 67095176 | 67095333 | 67095176 | ID=MS.gene81642.t1.exon5;Parent=MS.gene81642.t1 |
| chr5.1 | CDS | 67095176 | 67095333 | 67095176 | ID=cds.MS.gene81642.t1;Parent=MS.gene81642.t1 |
| chr5.1 | exon | 67096311 | 67096376 | 67096311 | ID=MS.gene81642.t1.exon6;Parent=MS.gene81642.t1 |
| chr5.1 | CDS | 67096311 | 67096376 | 67096311 | ID=cds.MS.gene81642.t1;Parent=MS.gene81642.t1 |
| chr5.1 | exon | 67097090 | 67097168 | 67097090 | ID=MS.gene81642.t1.exon7;Parent=MS.gene81642.t1 |
| chr5.1 | CDS | 67097090 | 67097168 | 67097090 | ID=cds.MS.gene81642.t1;Parent=MS.gene81642.t1 |
| chr5.1 | exon | 67098388 | 67098543 | 67098388 | ID=MS.gene81642.t1.exon8;Parent=MS.gene81642.t1 |
| chr5.1 | CDS | 67098388 | 67098543 | 67098388 | ID=cds.MS.gene81642.t1;Parent=MS.gene81642.t1 |
| chr5.1 | exon | 67098683 | 67098781 | 67098683 | ID=MS.gene81642.t1.exon9;Parent=MS.gene81642.t1 |
| chr5.1 | CDS | 67098683 | 67098781 | 67098683 | ID=cds.MS.gene81642.t1;Parent=MS.gene81642.t1 |
| chr5.1 | exon | 67101663 | 67101767 | 67101663 | ID=MS.gene81642.t1.exon10;Parent=MS.gene81642.t1 |
| chr5.1 | CDS | 67101663 | 67101767 | 67101663 | ID=cds.MS.gene81642.t1;Parent=MS.gene81642.t1 |
| chr5.1 | exon | 67102235 | 67102414 | 67102235 | ID=MS.gene81642.t1.exon11;Parent=MS.gene81642.t1 |
| chr5.1 | CDS | 67102235 | 67102414 | 67102235 | ID=cds.MS.gene81642.t1;Parent=MS.gene81642.t1 |
| chr5.1 | exon | 67102844 | 67102954 | 67102844 | ID=MS.gene81642.t1.exon12;Parent=MS.gene81642.t1 |
| chr5.1 | CDS | 67102844 | 67102954 | 67102844 | ID=cds.MS.gene81642.t1;Parent=MS.gene81642.t1 |
| chr5.1 | exon | 67103035 | 67103205 | 67103035 | ID=MS.gene81642.t1.exon13;Parent=MS.gene81642.t1 |
| chr5.1 | CDS | 67103035 | 67103205 | 67103035 | ID=cds.MS.gene81642.t1;Parent=MS.gene81642.t1 |
| Gene Sequence |
| Protein sequence |
