AlfalfaGEDB | MS.gene81727

M. sativa cultivar XinJiangDaYe / MS.gene81727

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81727.t1	XP_013468015.1	95.7	116	5	0	1	116	1	116	6.40E-56	226.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81727.t1	A0A072VJR4	95.7	116	5	0	1	116	1	116	4.6e-56	226.5

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050807	MS.gene81727	0.803299	3.73E-49	-1.69E-46
MS.gene051719	MS.gene81727	0.81983	9.45E-53	-1.69E-46
MS.gene051720	MS.gene81727	0.807674	4.50E-50	-1.69E-46
MS.gene053581	MS.gene81727	0.820059	8.38E-53	-1.69E-46
MS.gene056859	MS.gene81727	0.806764	7.02E-50	-1.69E-46

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PPI:

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81727.t1	MTR_1g060430	95.690	116	5	0	1	116	1	116	2.48e-79	229
MS.gene81727.t1	MTR_7g104250	64.762	105	25	3	10	113	1	94	1.45e-40	130
MS.gene81727.t1	MTR_8g043660	60.748	107	33	3	6	111	13	111	3.32e-35	117
MS.gene81727.t1	MTR_8g032890	55.140	107	39	3	6	111	13	111	3.86e-33	112
MS.gene81727.t1	MTR_1g101680	51.923	104	36	3	7	109	2	92	2.74e-31	107
MS.gene81727.t1	MTR_8g032940	55.140	107	39	3	6	111	13	111	6.63e-29	101
MS.gene81727.t1	MTR_1g101750	87.755	49	6	0	61	109	6	54	4.31e-28	97.8
MS.gene81727.t1	MTR_5g038200	50.000	48	24	0	54	101	27	74	7.31e-14	62.0
MS.gene81727.t1	MTR_7g055590	72.222	36	8	1	76	111	461	494	5.35e-11	58.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81727.t1	AT3G52790	56.075	107	42	2	6	111	5	107	5.73e-36	119
MS.gene81727.t1	AT5G62150	80.392	51	10	0	62	112	46	96	1.28e-27	97.8
MS.gene81727.t1	AT4G25433	75.510	49	12	0	62	110	57	105	9.97e-25	90.9

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Find 15 sgRNAs with CRISPR-Local

Find 14 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGGGTTAATCTTGATAACA+AGG	0.616888	1.3:-43809656	None:intergenic
ACTGTTTGTGGCATTGATGT+TGG	0.479292	1.3:+43809401	MS.gene81727:CDS
AGGCCAGGGAAAACATCATC+AGG	0.484403	1.3:-43809636	None:intergenic
ATGGAACTGTGCACTGTTTG+TGG	0.421993	1.3:+43809389	MS.gene81727:CDS
ATTTATGAACTAACAGAGAA+AGG	0.438055	1.3:-43809675	None:intergenic
CAATGGCAGAGAAGATTTCA+TGG	0.444015	1.3:+43809370	MS.gene81727:CDS
CACAAATCAGAAATGGCTTG+TGG	0.552081	1.3:+43809333	None:intergenic
GAGATATACGTTGTTGGCGA+AGG	0.561886	1.3:+43809540	MS.gene81727:CDS
GGATTCTCTTCAACAATATA+AGG	0.330783	1.3:-43809600	None:intergenic
TAATCTTGATAACAAGGCCA+GGG	0.622108	1.3:-43809650	None:intergenic

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CRISPR-GE

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badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	GGATTCTCTTCAACAATATA+AGG	-	chr1.3:43809603-43809622	None:intergenic	30.0%
	TCATCAGGATCATGAATATG+TGG	-	chr1.3:43809624-43809643	None:intergenic	35.0%
	TGTGATGAGATATACGTTGT+TGG	+	chr1.3:43809534-43809553	MS.gene81727:CDS	35.0%
	CAATGGCAGAGAAGATTTCA+TGG	+	chr1.3:43809370-43809389	MS.gene81727:CDS	40.0%
	TGGATGTTCATCAACAGCAA+TGG	+	chr1.3:43809353-43809372	MS.gene81727:CDS	40.0%
	ATGGAACTGTGCACTGTTTG+TGG	+	chr1.3:43809389-43809408	MS.gene81727:CDS	45.0%
	GAGATATACGTTGTTGGCGA+AGG	+	chr1.3:43809540-43809559	MS.gene81727:CDS	45.0%
	AGGCCAGGGAAAACATCATC+AGG	-	chr1.3:43809639-43809658	None:intergenic	50.0%
!	CAAACAATAAGTGAAAAATG+TGG	+	chr1.3:43809573-43809592	MS.gene81727:CDS	25.0%
!	AAGGGTTAATCTTGATAACA+AGG	-	chr1.3:43809659-43809678	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	43809345	43809695	43809345	ID=MS.gene81727
chr1.3	mRNA	43809345	43809695	43809345	ID=MS.gene81727.t1;Parent=MS.gene81727
chr1.3	exon	43809345	43809695	43809345	ID=MS.gene81727.t1.exon1;Parent=MS.gene81727.t1
chr1.3	CDS	43809345	43809695	43809345	ID=cds.MS.gene81727.t1;Parent=MS.gene81727.t1

Gene Sequence	>MS.gene81727 ATGGCTTGTGGATGTTCATCAACAGCAATGGCAGAGAAGATTTCATGGAACTGTGCACTGTTTGTGGCATTGATGTTGGTTTTGAGCTTCTGCGAATCAAACACAAGTGATGATGAGTTGAATGTTCAGATACAAAATATGCTTCAAGAGAACGATTTTAGTATTAGTAATGCGAACAACAACAAAGTGTGTGATGAGATATACGTTGTTGGCGAAGGAGAGACACTTCAAACAATAAGTGAAAAATGTGGTGATCCTTATATTGTTGAAGAGAATCCACATATTCATGATCCTGATGATGTTTTCCCTGGCCTTGTTATCAAGATTAACCCTTTCTCTGTTAGTTCATAA
Protein sequence	>MS.gene81727.t1 MACGCSSTAMAEKISWNCALFVALMLVLSFCESNTSDDELNVQIQNMLQENDFSISNANNNKVCDEIYVVGEGETLQTISEKCGDPYIVEENPHIHDPDDVFPGLVIKINPFSVSS