Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81727.t1 | XP_013468015.1 | 95.7 | 116 | 5 | 0 | 1 | 116 | 1 | 116 | 6.40E-56 | 226.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81727.t1 | A0A072VJR4 | 95.7 | 116 | 5 | 0 | 1 | 116 | 1 | 116 | 4.6e-56 | 226.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050807 | MS.gene81727 | 0.803299 | 3.73E-49 | -1.69E-46 |
| MS.gene051719 | MS.gene81727 | 0.81983 | 9.45E-53 | -1.69E-46 |
| MS.gene051720 | MS.gene81727 | 0.807674 | 4.50E-50 | -1.69E-46 |
| MS.gene053581 | MS.gene81727 | 0.820059 | 8.38E-53 | -1.69E-46 |
| MS.gene056859 | MS.gene81727 | 0.806764 | 7.02E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81727.t1 | MTR_1g060430 | 95.690 | 116 | 5 | 0 | 1 | 116 | 1 | 116 | 2.48e-79 | 229 |
| MS.gene81727.t1 | MTR_7g104250 | 64.762 | 105 | 25 | 3 | 10 | 113 | 1 | 94 | 1.45e-40 | 130 |
| MS.gene81727.t1 | MTR_8g043660 | 60.748 | 107 | 33 | 3 | 6 | 111 | 13 | 111 | 3.32e-35 | 117 |
| MS.gene81727.t1 | MTR_8g032890 | 55.140 | 107 | 39 | 3 | 6 | 111 | 13 | 111 | 3.86e-33 | 112 |
| MS.gene81727.t1 | MTR_1g101680 | 51.923 | 104 | 36 | 3 | 7 | 109 | 2 | 92 | 2.74e-31 | 107 |
| MS.gene81727.t1 | MTR_8g032940 | 55.140 | 107 | 39 | 3 | 6 | 111 | 13 | 111 | 6.63e-29 | 101 |
| MS.gene81727.t1 | MTR_1g101750 | 87.755 | 49 | 6 | 0 | 61 | 109 | 6 | 54 | 4.31e-28 | 97.8 |
| MS.gene81727.t1 | MTR_5g038200 | 50.000 | 48 | 24 | 0 | 54 | 101 | 27 | 74 | 7.31e-14 | 62.0 |
| MS.gene81727.t1 | MTR_7g055590 | 72.222 | 36 | 8 | 1 | 76 | 111 | 461 | 494 | 5.35e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81727.t1 | AT3G52790 | 56.075 | 107 | 42 | 2 | 6 | 111 | 5 | 107 | 5.73e-36 | 119 |
| MS.gene81727.t1 | AT5G62150 | 80.392 | 51 | 10 | 0 | 62 | 112 | 46 | 96 | 1.28e-27 | 97.8 |
| MS.gene81727.t1 | AT4G25433 | 75.510 | 49 | 12 | 0 | 62 | 110 | 57 | 105 | 9.97e-25 | 90.9 |
Find 15 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATTCTCTTCAACAATATA+AGG | 0.330783 | 1.3:-43809600 | None:intergenic |
| ATGGAACTGTGCACTGTTTG+TGG | 0.421993 | 1.3:+43809389 | MS.gene81727:CDS |
| ATTTATGAACTAACAGAGAA+AGG | 0.438055 | 1.3:-43809675 | None:intergenic |
| CAATGGCAGAGAAGATTTCA+TGG | 0.444015 | 1.3:+43809370 | MS.gene81727:CDS |
| ACTGTTTGTGGCATTGATGT+TGG | 0.479292 | 1.3:+43809401 | MS.gene81727:CDS |
| TTTATGAACTAACAGAGAAA+GGG | 0.480365 | 1.3:-43809674 | None:intergenic |
| AGGCCAGGGAAAACATCATC+AGG | 0.484403 | 1.3:-43809636 | None:intergenic |
| TTAATCTTGATAACAAGGCC+AGG | 0.525582 | 1.3:-43809651 | None:intergenic |
| CACAAATCAGAAATGGCTTG+TGG | 0.552081 | 1.3:+43809333 | None:intergenic |
| TCATCAGGATCATGAATATG+TGG | 0.554352 | 1.3:-43809621 | None:intergenic |
| TGTGATGAGATATACGTTGT+TGG | 0.555389 | 1.3:+43809534 | MS.gene81727:CDS |
| GAGATATACGTTGTTGGCGA+AGG | 0.561886 | 1.3:+43809540 | MS.gene81727:CDS |
| AAGGGTTAATCTTGATAACA+AGG | 0.616888 | 1.3:-43809656 | None:intergenic |
| TAATCTTGATAACAAGGCCA+GGG | 0.622108 | 1.3:-43809650 | None:intergenic |
| TGGATGTTCATCAACAGCAA+TGG | 0.630837 | 1.3:+43809353 | MS.gene81727:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CAAACAATAAGTGAAAAATG+TGG | + | chr1.3:43809573-43809592 | MS.gene81727:CDS | 25.0% |
| GGATTCTCTTCAACAATATA+AGG | - | chr1.3:43809603-43809622 | None:intergenic | 30.0% | |
| ! | AAGGGTTAATCTTGATAACA+AGG | - | chr1.3:43809659-43809678 | None:intergenic | 30.0% |
| TCATCAGGATCATGAATATG+TGG | - | chr1.3:43809624-43809643 | None:intergenic | 35.0% | |
| TGTGATGAGATATACGTTGT+TGG | + | chr1.3:43809534-43809553 | MS.gene81727:CDS | 35.0% | |
| ! | TAATCTTGATAACAAGGCCA+GGG | - | chr1.3:43809653-43809672 | None:intergenic | 35.0% |
| ! | TTAATCTTGATAACAAGGCC+AGG | - | chr1.3:43809654-43809673 | None:intergenic | 35.0% |
| CAATGGCAGAGAAGATTTCA+TGG | + | chr1.3:43809370-43809389 | MS.gene81727:CDS | 40.0% | |
| TGGATGTTCATCAACAGCAA+TGG | + | chr1.3:43809353-43809372 | MS.gene81727:CDS | 40.0% | |
| !! | ACTGTTTGTGGCATTGATGT+TGG | + | chr1.3:43809401-43809420 | MS.gene81727:CDS | 40.0% |
| ATGGAACTGTGCACTGTTTG+TGG | + | chr1.3:43809389-43809408 | MS.gene81727:CDS | 45.0% | |
| GAGATATACGTTGTTGGCGA+AGG | + | chr1.3:43809540-43809559 | MS.gene81727:CDS | 45.0% | |
| !! | GATCCTGATGATGTTTTCCC+TGG | + | chr1.3:43809633-43809652 | MS.gene81727:CDS | 45.0% |
| AGGCCAGGGAAAACATCATC+AGG | - | chr1.3:43809639-43809658 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 43809345 | 43809695 | 43809345 | ID=MS.gene81727 |
| chr1.3 | mRNA | 43809345 | 43809695 | 43809345 | ID=MS.gene81727.t1;Parent=MS.gene81727 |
| chr1.3 | exon | 43809345 | 43809695 | 43809345 | ID=MS.gene81727.t1.exon1;Parent=MS.gene81727.t1 |
| chr1.3 | CDS | 43809345 | 43809695 | 43809345 | ID=cds.MS.gene81727.t1;Parent=MS.gene81727.t1 |
| Gene Sequence |
| Protein sequence |
