AlfalfaGEDB | MS.gene056859

M. sativa cultivar XinJiangDaYe / MS.gene056859

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056859.t1	XP_013468015.1	94.8	116	6	0	1	116	1	116	1.10E-55	225.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056859.t1	A0A072VJR4	94.8	116	6	0	1	116	1	116	7.9e-56	225.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049404	MS.gene056859	0.800266	1.56E-48	-1.69E-46
MS.gene050807	MS.gene056859	0.82177	3.38E-53	-1.69E-46
MS.gene051719	MS.gene056859	0.838886	2.20E-57	-1.69E-46
MS.gene051720	MS.gene056859	0.835201	1.93E-56	-1.69E-46
MS.gene05415	MS.gene056859	0.809014	2.33E-50	-1.69E-46
MS.gene055466	MS.gene056859	0.801756	7.75E-49	-1.69E-46
MS.gene056859	MS.gene057060	0.800147	1.65E-48	-1.69E-46
MS.gene056859	MS.gene059015	0.807569	4.74E-50	-1.69E-46
MS.gene056859	MS.gene060519	0.810016	1.42E-50	-1.69E-46
MS.gene056859	MS.gene061947	0.81823	2.19E-52	-1.69E-46
MS.gene056859	MS.gene062821	0.810647	1.04E-50	-1.69E-46
MS.gene056859	MS.gene063163	0.809613	1.73E-50	-1.69E-46
MS.gene056859	MS.gene064944	0.844817	5.98E-59	-1.69E-46
MS.gene056859	MS.gene065667	0.809419	1.91E-50	-1.69E-46
MS.gene056859	MS.gene066335	0.800898	1.16E-48	-1.69E-46
MS.gene056859	MS.gene067071	0.800816	1.21E-48	-1.69E-46
MS.gene056859	MS.gene067932	0.826714	2.33E-54	-1.69E-46
MS.gene056859	MS.gene068004	0.821097	4.84E-53	-1.69E-46
MS.gene056859	MS.gene068006	0.804088	2.55E-49	-1.69E-46
MS.gene056859	MS.gene068116	0.803731	3.03E-49	-1.69E-46
MS.gene056859	MS.gene068924	0.806286	8.86E-50	-1.69E-46
MS.gene056859	MS.gene069956	0.806645	7.44E-50	-1.69E-46
MS.gene056859	MS.gene073083	0.802631	5.12E-49	-1.69E-46
MS.gene056859	MS.gene23105	0.820845	5.53E-53	-1.69E-46
MS.gene056859	MS.gene24885	0.800043	1.73E-48	-1.69E-46
MS.gene056859	MS.gene29576	0.812451	4.21E-51	-1.69E-46
MS.gene056859	MS.gene29807	0.806747	7.08E-50	-1.69E-46
MS.gene056859	MS.gene30882	0.804558	2.04E-49	-1.69E-46
MS.gene056859	MS.gene31347	0.808011	3.82E-50	-1.69E-46
MS.gene056859	MS.gene31433	0.806793	6.92E-50	-1.69E-46
MS.gene056859	MS.gene34180	0.817053	4.03E-52	-1.69E-46
MS.gene056859	MS.gene35575	0.801383	9.24E-49	-1.69E-46
MS.gene056859	MS.gene37745	0.804171	2.46E-49	-1.69E-46
MS.gene056859	MS.gene43075	0.815446	9.21E-52	-1.69E-46
MS.gene056859	MS.gene47689	0.804274	2.34E-49	-1.69E-46
MS.gene056859	MS.gene53586	0.874974	4.44E-68	-1.69E-46
MS.gene056859	MS.gene54507	0.805304	1.42E-49	-1.69E-46
MS.gene056859	MS.gene64249	0.865959	4.05E-65	-1.69E-46
MS.gene056859	MS.gene65172	0.825584	4.33E-54	-1.69E-46
MS.gene056859	MS.gene67215	0.804924	1.71E-49	-1.69E-46
MS.gene056859	MS.gene68886	0.804477	2.12E-49	-1.69E-46
MS.gene056859	MS.gene71704	0.808683	2.75E-50	-1.69E-46
MS.gene056859	MS.gene72268	0.866094	3.67E-65	-1.69E-46
MS.gene056859	MS.gene73044	0.810359	1.20E-50	-1.69E-46
MS.gene056859	MS.gene76158	0.805033	1.62E-49	-1.69E-46
MS.gene056859	MS.gene79960	0.806571	7.71E-50	-1.69E-46
MS.gene056859	MS.gene80853	0.805539	1.27E-49	-1.69E-46
MS.gene056859	MS.gene81466	0.81215	4.90E-51	-1.69E-46
MS.gene056859	MS.gene81727	0.806764	7.02E-50	-1.69E-46
MS.gene056859	MS.gene82699	0.803962	2.71E-49	-1.69E-46
MS.gene056859	MS.gene84426	0.827188	1.80E-54	-1.69E-46
MS.gene056859	MS.gene84803	0.801342	9.42E-49	-1.69E-46
MS.gene056859	MS.gene88243	0.817712	2.86E-52	-1.69E-46
MS.gene056859	MS.gene88304	0.807583	4.71E-50	-1.69E-46
MS.gene056859	MS.gene90649	0.808577	2.89E-50	-1.69E-46
MS.gene056859	MS.gene94929	0.821903	3.15E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056859.t1	MTR_1g060430	94.828	116	6	0	1	116	1	116	1.02e-78	227
MS.gene056859.t1	MTR_7g104250	65.714	105	24	3	10	113	1	94	2.49e-41	132
MS.gene056859.t1	MTR_8g043660	60.748	107	33	3	6	111	13	111	5.14e-35	117
MS.gene056859.t1	MTR_8g032890	55.140	107	39	3	6	111	13	111	6.17e-33	111
MS.gene056859.t1	MTR_1g101680	51.456	103	38	2	7	109	2	92	3.34e-31	107
MS.gene056859.t1	MTR_8g032940	55.140	107	39	3	6	111	13	111	1.49e-28	100
MS.gene056859.t1	MTR_1g101750	87.755	49	6	0	61	109	6	54	4.55e-28	97.8
MS.gene056859.t1	MTR_5g038200	50.000	48	24	0	54	101	27	74	7.88e-14	62.0
MS.gene056859.t1	MTR_7g055590	72.222	36	8	1	76	111	461	494	5.51e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056859.t1	AT3G52790	56.604	106	43	1	6	111	5	107	1.06e-36	120
MS.gene056859.t1	AT5G62150	77.358	53	12	0	60	112	44	96	1.32e-27	97.8
MS.gene056859.t1	AT4G25433	75.510	49	12	0	62	110	57	105	1.08e-24	90.5

Find 15 sgRNAs with CRISPR-Local

Find 14 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGATTCTCTTCAACAATATA+AGG	0.330783	1.1:-45978942	None:intergenic
ATTTATGAACTAACAGAGAA+AGG	0.438055	1.1:-45979017	None:intergenic
CAATGGCAGAGAAGATTTCA+TGG	0.444015	1.1:+45978712	MS.gene056859:CDS
ATGGAACTGTGCGTTGTTTG+TGG	0.460242	1.1:+45978731	MS.gene056859:CDS
TTTATGAACTAACAGAGAAA+GGG	0.480365	1.1:-45979016	None:intergenic
AGGCCAGGGAAAACATCATC+AGG	0.484403	1.1:-45978978	None:intergenic
TTAATCTTGATAACAAGGCC+AGG	0.525582	1.1:-45978993	None:intergenic
GTTGTTTGTGGCATTGATGT+TGG	0.526384	1.1:+45978743	MS.gene056859:CDS
TCATCAGGATCATGAATATG+TGG	0.554352	1.1:-45978963	None:intergenic
TGTGATGAGATATACGTTGT+TGG	0.555389	1.1:+45978876	MS.gene056859:CDS
CACATATCAGAAATGGCTTG+TGG	0.561139	1.1:+45978675	None:intergenic
GAGATATACGTTGTTGGCGA+AGG	0.561886	1.1:+45978882	MS.gene056859:CDS
AAGGGTTAATCTTGATAACA+AGG	0.616888	1.1:-45978998	None:intergenic
TAATCTTGATAACAAGGCCA+GGG	0.622108	1.1:-45978992	None:intergenic
TGGATGTTCATCAACAGCAA+TGG	0.630837	1.1:+45978695	MS.gene056859:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CAAACAATAAGTGAAAAATG+TGG	+	chr1.1:45978915-45978934	MS.gene056859:CDS	25.0%
	GGATTCTCTTCAACAATATA+AGG	-	chr1.1:45978945-45978964	None:intergenic	30.0%
!	AAGGGTTAATCTTGATAACA+AGG	-	chr1.1:45979001-45979020	None:intergenic	30.0%
	TCATCAGGATCATGAATATG+TGG	-	chr1.1:45978966-45978985	None:intergenic	35.0%
	TGTGATGAGATATACGTTGT+TGG	+	chr1.1:45978876-45978895	MS.gene056859:CDS	35.0%
!	TAATCTTGATAACAAGGCCA+GGG	-	chr1.1:45978995-45979014	None:intergenic	35.0%
!	TTAATCTTGATAACAAGGCC+AGG	-	chr1.1:45978996-45979015	None:intergenic	35.0%
	CAATGGCAGAGAAGATTTCA+TGG	+	chr1.1:45978712-45978731	MS.gene056859:CDS	40.0%
	TGGATGTTCATCAACAGCAA+TGG	+	chr1.1:45978695-45978714	MS.gene056859:CDS	40.0%
!!	GTTGTTTGTGGCATTGATGT+TGG	+	chr1.1:45978743-45978762	MS.gene056859:CDS	40.0%
	ATGGAACTGTGCGTTGTTTG+TGG	+	chr1.1:45978731-45978750	MS.gene056859:CDS	45.0%
	GAGATATACGTTGTTGGCGA+AGG	+	chr1.1:45978882-45978901	MS.gene056859:CDS	45.0%
!!	GATCCTGATGATGTTTTCCC+TGG	+	chr1.1:45978975-45978994	MS.gene056859:CDS	45.0%
	AGGCCAGGGAAAACATCATC+AGG	-	chr1.1:45978981-45979000	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	45978687	45979037	45978687	ID=MS.gene056859
chr1.1	mRNA	45978687	45979037	45978687	ID=MS.gene056859.t1;Parent=MS.gene056859
chr1.1	exon	45978687	45979037	45978687	ID=MS.gene056859.t1.exon1;Parent=MS.gene056859.t1
chr1.1	CDS	45978687	45979037	45978687	ID=cds.MS.gene056859.t1;Parent=MS.gene056859.t1

Gene Sequence	>MS.gene056859 ATGGCTTGTGGATGTTCATCAACAGCAATGGCAGAGAAGATTTCATGGAACTGTGCGTTGTTTGTGGCATTGATGTTGGTTTTGAACTTCTGTGAATCAAACACAAGTGATGATGAGTTGAATGTTCAGATACAAAATATGCTTCAAGAGAACGATTTTAGTATTAGTAATGCGAACAACAACAAAGTGTGTGATGAGATATACGTTGTTGGCGAAGGAGAGACACTTCAAACAATAAGTGAAAAATGTGGTGATCCTTATATTGTTGAAGAGAATCCACATATTCATGATCCTGATGATGTTTTCCCTGGCCTTGTTATCAAGATTAACCCTTTCTCTGTTAGTTCATAA
Protein sequence	>MS.gene056859.t1 MACGCSSTAMAEKISWNCALFVALMLVLNFCESNTSDDELNVQIQNMLQENDFSISNANNNKVCDEIYVVGEGETLQTISEKCGDPYIVEENPHIHDPDDVFPGLVIKINPFSVSS