AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82004

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82004.t1 MTR_8g024250 95.088 285 10 2 1 285 1 281 0.0 500
MS.gene82004.t1 MTR_7g118270 63.243 185 68 0 100 284 44 228 1.01e-88 264
MS.gene82004.t1 MTR_7g106050 37.013 154 97 0 106 259 24 177 1.07e-29 112
MS.gene82004.t1 MTR_4g051880 34.507 142 85 1 126 259 45 186 6.65e-22 91.7
MS.gene82004.t1 MTR_3g092790 33.333 150 99 1 125 274 49 197 1.42e-21 90.5
MS.gene82004.t1 MTR_2g086790 30.233 172 107 3 100 259 17 187 2.67e-20 87.4
MS.gene82004.t1 MTR_1g057420 30.464 151 105 0 121 271 39 189 4.33e-20 86.3
MS.gene82004.t1 MTR_2g011350 24.378 201 139 4 70 268 1 190 1.88e-15 73.6
MS.gene82004.t1 MTR_5g087760 26.705 176 117 6 99 263 60 234 1.84e-12 66.2
MS.gene82004.t1 MTR_4g119428 25.658 152 109 3 112 261 65 214 9.99e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82004.t1 AT4G01410 49.198 187 95 0 99 285 41 227 1.18e-56 182
MS.gene82004.t1 AT3G44220 29.762 168 118 0 109 276 27 194 1.56e-24 98.2
MS.gene82004.t1 AT3G52470 30.857 175 115 4 100 271 17 188 2.46e-24 97.8
MS.gene82004.t1 AT5G53730 33.557 149 97 1 113 259 38 186 3.00e-24 97.8
MS.gene82004.t1 AT2G35960 30.968 155 107 0 117 271 36 190 2.22e-23 95.5
MS.gene82004.t1 AT5G22200 32.692 156 105 0 121 276 43 198 3.93e-23 94.7
MS.gene82004.t1 AT5G06330 35.220 159 93 7 121 273 39 193 6.99e-23 94.0
MS.gene82004.t1 AT3G11660 33.735 166 101 7 99 259 16 177 1.28e-22 93.2
MS.gene82004.t1 AT4G09590 29.814 161 113 0 103 263 23 183 2.46e-22 92.4
MS.gene82004.t1 AT2G35970 30.435 161 112 0 103 263 23 183 9.35e-22 90.9
MS.gene82004.t1 AT5G22870 27.933 179 122 2 88 260 9 186 1.74e-16 76.6
MS.gene82004.t1 AT4G05220 24.405 168 125 2 103 270 47 212 1.48e-15 74.3
MS.gene82004.t1 AT1G61760 27.374 179 127 2 103 280 45 221 5.23e-15 72.8
MS.gene82004.t1 AT2G35980 27.848 158 113 1 106 262 45 202 1.21e-12 66.2

Find 73 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACGTGGTTGTTCCATTTC+TGG 0.141448 8.2:-71550502 None:intergenic
GTGGGTCAGATTCCTCTTAT+TGG 0.186108 8.2:+71551150 MS.gene82004:CDS
TGACGTGGTTGTTCCATTTC+GGG 0.190056 8.2:-71550460 None:intergenic
TTGACGTGGTTGTTCCATTT+CGG 0.237511 8.2:-71550461 None:intergenic
GCATTATAATCCTCATCTTA+TGG 0.245398 8.2:+71550683 MS.gene82004:CDS
CATATGCAATGTTCACGTTT+TGG 0.280824 8.2:-71550634 None:intergenic
TTGTCGCGGTTGTTCCATTT+CGG 0.294802 8.2:-71550545 None:intergenic
TGTCGCGGTTGTTCCATTTC+GGG 0.304192 8.2:-71550544 None:intergenic
TTGGTGTTGGATGAGAATTA+TGG 0.323767 8.2:+71551006 MS.gene82004:CDS
TGGTGTTGGATGAGAATTAT+GGG 0.333006 8.2:+71551007 MS.gene82004:CDS
TAAATGGATACACGTTTGTT+TGG 0.340333 8.2:-71550826 None:intergenic
AGCATATGATGGGGCGTAAT+CGG 0.346812 8.2:-71550889 None:intergenic
TTCAAAACATCCATCGAAAC+TGG 0.348821 8.2:-71550979 None:intergenic
GACGTTGTGAATGGTGTTCT+TGG 0.356343 8.2:-71550387 None:intergenic
GGAAAGCCTGTCAAAGTAAA+TGG 0.359178 8.2:-71550842 None:intergenic
TATGCTTCCGTCGCTTTACT+TGG 0.361015 8.2:+71550906 MS.gene82004:CDS
GTCACAAGTGCTGCAATCTA+TGG 0.378150 8.2:+71550739 MS.gene82004:CDS
GTTCCATTTCGGGATATTGA+TGG 0.381347 8.2:-71550534 None:intergenic
AACCATACCTTTGTGTTCCC+TGG 0.386231 8.2:-71550587 None:intergenic
ACAATCTTCTTCCTCGTACT+TGG 0.390574 8.2:+71550661 MS.gene82004:CDS
ACCGGCACGCCTCCTAAAAC+TGG 0.405172 8.2:-71550955 None:intergenic
GCCAAGTGTGATTTATTGTT+GGG 0.416075 8.2:+71551111 MS.gene82004:CDS
TGTGAATGGTGTTCTTGGTT+TGG 0.417912 8.2:-71550382 None:intergenic
GGCAGGTTGAGGTGGAAAAC+TGG 0.421371 8.2:+71551057 MS.gene82004:CDS
GGATGAGAATTATGGGGTGG+TGG 0.422334 8.2:+71551014 MS.gene82004:CDS
TGCCAAGTGTGATTTATTGT+TGG 0.432416 8.2:+71551110 MS.gene82004:CDS
GTGTATCCATTTACTTTGAC+AGG 0.438624 8.2:+71550836 MS.gene82004:CDS
TTATTGTTGGGTTTGAAGAA+AGG 0.446899 8.2:+71551123 MS.gene82004:CDS
ACGTCATCAATATCCAGAAA+TGG 0.451333 8.2:+71550489 MS.gene82004:CDS
GATGAGAATTATGGGGTGGT+GGG 0.453278 8.2:+71551015 MS.gene82004:CDS
TGCAATTCACAATCTTCATA+AGG 0.457022 8.2:+71550800 MS.gene82004:CDS
ACGTTGTCAATACCCCGAAA+TGG 0.457812 8.2:+71550447 MS.gene82004:CDS
TATGTATCCACATCACAAAC+TGG 0.461285 8.2:-71551177 None:intergenic
ACGCCATCAATATCCCGAAA+TGG 0.481535 8.2:+71550531 MS.gene82004:CDS
TGAAACTTATATTTCAAGGC+AGG 0.482813 8.2:+71551040 MS.gene82004:CDS
GGTGTGAAACTTATATTTCA+AGG 0.486868 8.2:+71551036 MS.gene82004:CDS
CAAACTGGAGCACCAATAAG+AGG 0.488904 8.2:-71551162 None:intergenic
ACCATACCTTTGTGTTCCCT+GGG 0.499208 8.2:-71550586 None:intergenic
ACATCCATCGAAACTGGAAC+CGG 0.504452 8.2:-71550973 None:intergenic
GGTTTGAAGAAAGGTCTTGT+GGG 0.505112 8.2:+71551132 MS.gene82004:CDS
TTATATTTCAAGGCAGGTTG+AGG 0.505575 8.2:+71551046 MS.gene82004:CDS
AAAGCGACGGAAGCATATGA+TGG 0.516968 8.2:-71550900 None:intergenic
GGGTTTGAAGAAAGGTCTTG+TGG 0.531873 8.2:+71551131 MS.gene82004:CDS
TGCATTGAGATTGACATGTC+TGG 0.540818 8.2:-71550781 None:intergenic
GGTGTTGGATGAGAATTATG+GGG 0.556537 8.2:+71551008 MS.gene82004:CDS
TATTTCAAGGCAGGTTGAGG+TGG 0.558650 8.2:+71551049 MS.gene82004:CDS
TCCCAGGGAACACAAAGGTA+TGG 0.559531 8.2:+71550585 MS.gene82004:CDS
TGGTGCTCCAGTTTGTGATG+TGG 0.562878 8.2:+71551170 MS.gene82004:CDS
TAGGGAAATGACGTTGTGAA+TGG 0.563199 8.2:-71550396 None:intergenic
AAGCGACGGAAGCATATGAT+GGG 0.563769 8.2:-71550899 None:intergenic
CGTGCCGGTTCCAGTTTCGA+TGG 0.565718 8.2:+71550969 MS.gene82004:CDS
AACAACGACATCAATATCCC+AGG 0.569261 8.2:+71550569 MS.gene82004:CDS
ACCCAACAATAAATCACACT+TGG 0.575443 8.2:-71551112 None:intergenic
TGCTTCTCCAAGTAAAGCGA+CGG 0.596510 8.2:-71550913 None:intergenic
TATGATGGGGCGTAATCGGA+TGG 0.600494 8.2:-71550885 None:intergenic
GACGTGGTTGTTCCATTTCG+GGG 0.622072 8.2:-71550459 None:intergenic
TGATGGCGTTGTTGTTGACG+TGG 0.628102 8.2:-71550517 None:intergenic
GCACTTGTGACTGTGATGTG+AGG 0.630907 8.2:-71550727 None:intergenic
ACAACGACATCAATATCCCA+GGG 0.646531 8.2:+71550570 MS.gene82004:CDS
TGATGACGTTGTTGTTGACG+TGG 0.649666 8.2:-71550475 None:intergenic
GGTATGCAAGCCATAAGATG+AGG 0.669740 8.2:-71550693 None:intergenic
ATGGTTTCTATATGACACAA+AGG 0.671270 8.2:-71550866 None:intergenic
TGATGTCGTTGTTGTTGTCG+CGG 0.672627 8.2:-71550559 None:intergenic
GGATTATAATGCCAAGTACG+AGG 0.674453 8.2:-71550672 None:intergenic
GTTGGATGAGAATTATGGGG+TGG 0.688698 8.2:+71551011 MS.gene82004:CDS
GGAGAAGCATGAAACCGTGT+CGG 0.692283 8.2:+71550927 MS.gene82004:CDS
AGCGACGGAAGCATATGATG+GGG 0.695019 8.2:-71550898 None:intergenic
TAAAACTGGTGATACCGACA+CGG 0.699605 8.2:-71550941 None:intergenic
CAATATCCCAGGGAACACAA+AGG 0.701119 8.2:+71550580 MS.gene82004:CDS
ATTGAGATTGACATGTCTGG+TGG 0.701504 8.2:-71550778 None:intergenic
GTTTCTATATGACACAAAGG+CGG 0.718743 8.2:-71550863 None:intergenic
GCATTAGGTACTCTATAACG+AGG 0.741839 8.2:-71550610 None:intergenic
CTGGTGGTGAAGTTGCATTG+AGG 0.741850 8.2:-71550762 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATGGTTTCTATATGACACAA+AGG - chr8.2:71550869-71550888 None:intergenic 30.0%
GCATTATAATCCTCATCTTA+TGG + chr8.2:71550683-71550702 MS.gene82004:CDS 30.0%
GGTGTGAAACTTATATTTCA+AGG + chr8.2:71551036-71551055 MS.gene82004:CDS 30.0%
TAAATGGATACACGTTTGTT+TGG - chr8.2:71550829-71550848 None:intergenic 30.0%
TGAAACTTATATTTCAAGGC+AGG + chr8.2:71551040-71551059 MS.gene82004:CDS 30.0%
TGCAATTCACAATCTTCATA+AGG + chr8.2:71550800-71550819 MS.gene82004:CDS 30.0%
TTATTGTTGGGTTTGAAGAA+AGG + chr8.2:71551123-71551142 MS.gene82004:CDS 30.0%
ACCCAACAATAAATCACACT+TGG - chr8.2:71551115-71551134 None:intergenic 35.0%
ACGTCATCAATATCCAGAAA+TGG + chr8.2:71550489-71550508 MS.gene82004:CDS 35.0%
GCCAAGTGTGATTTATTGTT+GGG + chr8.2:71551111-71551130 MS.gene82004:CDS 35.0%
GTGTATCCATTTACTTTGAC+AGG + chr8.2:71550836-71550855 MS.gene82004:CDS 35.0%
GTTTCTATATGACACAAAGG+CGG - chr8.2:71550866-71550885 None:intergenic 35.0%
TATGTATCCACATCACAAAC+TGG - chr8.2:71551180-71551199 None:intergenic 35.0%
TGCCAAGTGTGATTTATTGT+TGG + chr8.2:71551110-71551129 MS.gene82004:CDS 35.0%
TTATATTTCAAGGCAGGTTG+AGG + chr8.2:71551046-71551065 MS.gene82004:CDS 35.0%
TTCAAAACATCCATCGAAAC+TGG - chr8.2:71550982-71551001 None:intergenic 35.0%
! CATATGCAATGTTCACGTTT+TGG - chr8.2:71550637-71550656 None:intergenic 35.0%
! GTTGTTGTGGCATTTTGTTA+GGG - chr8.2:71550417-71550436 None:intergenic 35.0%
! TGGTGTTGGATGAGAATTAT+GGG + chr8.2:71551007-71551026 MS.gene82004:CDS 35.0%
! TGTTGTTGTGGCATTTTGTT+AGG - chr8.2:71550418-71550437 None:intergenic 35.0%
!! GATGGATGTTTTGAATGGTT+TGG + chr8.2:71550987-71551006 MS.gene82004:CDS 35.0%
!! GTTTCGATGGATGTTTTGAA+TGG + chr8.2:71550982-71551001 MS.gene82004:CDS 35.0%
!! TTGGTGTTGGATGAGAATTA+TGG + chr8.2:71551006-71551025 MS.gene82004:CDS 35.0%
!!! TGTTTTGAATGGTTTGGTGT+TGG + chr8.2:71550993-71551012 MS.gene82004:CDS 35.0%
AACAACGACATCAATATCCC+AGG + chr8.2:71550569-71550588 MS.gene82004:CDS 40.0%
ACAACGACATCAATATCCCA+GGG + chr8.2:71550570-71550589 MS.gene82004:CDS 40.0%
ACAATCTTCTTCCTCGTACT+TGG + chr8.2:71550661-71550680 MS.gene82004:CDS 40.0%
ATTGAGATTGACATGTCTGG+TGG - chr8.2:71550781-71550800 None:intergenic 40.0%
GCATTAGGTACTCTATAACG+AGG - chr8.2:71550613-71550632 None:intergenic 40.0%
GGAAAGCCTGTCAAAGTAAA+TGG - chr8.2:71550845-71550864 None:intergenic 40.0%
GGATTATAATGCCAAGTACG+AGG - chr8.2:71550675-71550694 None:intergenic 40.0%
GTTCCATTTCGGGATATTGA+TGG - chr8.2:71550537-71550556 None:intergenic 40.0%
TAAAACTGGTGATACCGACA+CGG - chr8.2:71550944-71550963 None:intergenic 40.0%
TAGGGAAATGACGTTGTGAA+TGG - chr8.2:71550399-71550418 None:intergenic 40.0%
TGCATTGAGATTGACATGTC+TGG - chr8.2:71550784-71550803 None:intergenic 40.0%
TGTGAATGGTGTTCTTGGTT+TGG - chr8.2:71550385-71550404 None:intergenic 40.0%
TTGACGTGGTTGTTCCATTT+CGG - chr8.2:71550464-71550483 None:intergenic 40.0%
! CGTTGTTGTTGTTGTTGTTG+TGG - chr8.2:71550430-71550449 None:intergenic 40.0%
! GGTGTTGGATGAGAATTATG+GGG + chr8.2:71551008-71551027 MS.gene82004:CDS 40.0%
! GGTTTGAAGAAAGGTCTTGT+GGG + chr8.2:71551132-71551151 MS.gene82004:CDS 40.0%
!! ACTGTGATGTGAGGTTTTGA+GGG - chr8.2:71550721-71550740 None:intergenic 40.0%
!!! TGTTCACGTTTTGGAGCATT+AGG - chr8.2:71550628-71550647 None:intergenic 40.0%
AACCATACCTTTGTGTTCCC+TGG - chr8.2:71550590-71550609 None:intergenic 45.0%
ACATCCATCGAAACTGGAAC+CGG - chr8.2:71550976-71550995 None:intergenic 45.0%
ACCATACCTTTGTGTTCCCT+GGG - chr8.2:71550589-71550608 None:intergenic 45.0%
ACGCCATCAATATCCCGAAA+TGG + chr8.2:71550531-71550550 MS.gene82004:CDS 45.0%
ACGTTGTCAATACCCCGAAA+TGG + chr8.2:71550447-71550466 MS.gene82004:CDS 45.0%
CAATATCCCAGGGAACACAA+AGG + chr8.2:71550580-71550599 MS.gene82004:CDS 45.0%
GACGTTGTGAATGGTGTTCT+TGG - chr8.2:71550390-71550409 None:intergenic 45.0%
GATGAGAATTATGGGGTGGT+GGG + chr8.2:71551015-71551034 MS.gene82004:CDS 45.0%
GGGTTTGAAGAAAGGTCTTG+TGG + chr8.2:71551131-71551150 MS.gene82004:CDS 45.0%
GGTATGCAAGCCATAAGATG+AGG - chr8.2:71550696-71550715 None:intergenic 45.0%
GTGGGTCAGATTCCTCTTAT+TGG + chr8.2:71551150-71551169 MS.gene82004:CDS 45.0%
GTTGGATGAGAATTATGGGG+TGG + chr8.2:71551011-71551030 MS.gene82004:CDS 45.0%
TGACGTGGTTGTTCCATTTC+GGG - chr8.2:71550463-71550482 None:intergenic 45.0%
TGACGTGGTTGTTCCATTTC+TGG - chr8.2:71550463-71550482 None:intergenic 45.0%
TGCTTCTCCAAGTAAAGCGA+CGG - chr8.2:71550916-71550935 None:intergenic 45.0%
TTGTCGCGGTTGTTCCATTT+CGG - chr8.2:71550548-71550567 None:intergenic 45.0%
! AAAGCGACGGAAGCATATGA+TGG - chr8.2:71550903-71550922 None:intergenic 45.0%
! AAGCGACGGAAGCATATGAT+GGG - chr8.2:71550902-71550921 None:intergenic 45.0%
! AGCATATGATGGGGCGTAAT+CGG - chr8.2:71550892-71550911 None:intergenic 45.0%
! CAAACTGGAGCACCAATAAG+AGG - chr8.2:71551165-71551184 None:intergenic 45.0%
! GACTGTGATGTGAGGTTTTG+AGG - chr8.2:71550722-71550741 None:intergenic 45.0%
! GTCACAAGTGCTGCAATCTA+TGG + chr8.2:71550739-71550758 MS.gene82004:CDS 45.0%
! TATGCTTCCGTCGCTTTACT+TGG + chr8.2:71550906-71550925 MS.gene82004:CDS 45.0%
! TATTTCAAGGCAGGTTGAGG+TGG + chr8.2:71551049-71551068 MS.gene82004:CDS 45.0%
! TGATGACGTTGTTGTTGACG+TGG - chr8.2:71550478-71550497 None:intergenic 45.0%
! TGATGTCGTTGTTGTTGTCG+CGG - chr8.2:71550562-71550581 None:intergenic 45.0%
!! GTGTCGGTATCACCAGTTTT+AGG + chr8.2:71550943-71550962 MS.gene82004:CDS 45.0%
!!! TCGGTATCACCAGTTTTAGG+AGG + chr8.2:71550946-71550965 MS.gene82004:CDS 45.0%
!!! TGATGTGAGGTTTTGAGGGA+TGG - chr8.2:71550717-71550736 None:intergenic 45.0%
GACGTGGTTGTTCCATTTCG+GGG - chr8.2:71550462-71550481 None:intergenic 50.0%
GCACTTGTGACTGTGATGTG+AGG - chr8.2:71550730-71550749 None:intergenic 50.0%
GGATGAGAATTATGGGGTGG+TGG + chr8.2:71551014-71551033 MS.gene82004:CDS 50.0%
TCCCAGGGAACACAAAGGTA+TGG + chr8.2:71550585-71550604 MS.gene82004:CDS 50.0%
TGGTGCTCCAGTTTGTGATG+TGG + chr8.2:71551170-71551189 MS.gene82004:CDS 50.0%
TGTCGCGGTTGTTCCATTTC+GGG - chr8.2:71550547-71550566 None:intergenic 50.0%
! AGCGACGGAAGCATATGATG+GGG - chr8.2:71550901-71550920 None:intergenic 50.0%
! CTGGTGGTGAAGTTGCATTG+AGG - chr8.2:71550765-71550784 None:intergenic 50.0%
! GGAGAAGCATGAAACCGTGT+CGG + chr8.2:71550927-71550946 MS.gene82004:CDS 50.0%
! TATGATGGGGCGTAATCGGA+TGG - chr8.2:71550888-71550907 None:intergenic 50.0%
! TGATGGCGTTGTTGTTGACG+TGG - chr8.2:71550520-71550539 None:intergenic 50.0%
! GGCAGGTTGAGGTGGAAAAC+TGG + chr8.2:71551057-71551076 MS.gene82004:CDS 55.0%
!!! ACCAGTTTTAGGAGGCGTGC+CGG + chr8.2:71550954-71550973 MS.gene82004:CDS 55.0%
ACCGGCACGCCTCCTAAAAC+TGG - chr8.2:71550958-71550977 None:intergenic 60.0%
!! CGTGCCGGTTCCAGTTTCGA+TGG + chr8.2:71550969-71550988 MS.gene82004:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 71550343 71551200 71550343 ID=MS.gene82004
chr8.2 mRNA 71550343 71551200 71550343 ID=MS.gene82004.t1;Parent=MS.gene82004
chr8.2 exon 71550343 71551200 71550343 ID=MS.gene82004.t1.exon1;Parent=MS.gene82004.t1
chr8.2 CDS 71550343 71551200 71550343 ID=cds.MS.gene82004.t1;Parent=MS.gene82004.t1
Gene Sequence

>MS.gene82004

ATGGAAGATGAAATAATGCATATAGAACGTCAAGAAGATCCAAACCAAGAACACCATTCACAACGTCATTTCCCTAACAAAATGCCACAACAACAACAACAACAACGTTGTCAATACCCCGAAATGGAACAACCACGTCAACAACAACGTCATCAATATCCAGAAATGGAACAACCACGTCAACAACAACGCCATCAATATCCCGAAATGGAACAACCGCGACAACAACAACGACATCAATATCCCAGGGAACACAAAGGTATGGTTCCTCGTTATAGAGTACCTAATGCTCCAAAACGTGAACATTGCATATGCATTACAATCTTCTTCCTCGTACTTGGCATTATAATCCTCATCTTATGGCTTGCATACCATCCCTCAAAACCTCACATCACAGTCACAAGTGCTGCAATCTATGGCCTCAATGCAACTTCACCACCAGACATGTCAATCTCAATGCAATTCACAATCTTCATAAGGAATCCAAACAAACGTGTATCCATTTACTTTGACAGGCTTTCCGCCTTTGTGTCATATAGAAACCATCCGATTACGCCCCATCATATGCTTCCGTCGCTTTACTTGGAGAAGCATGAAACCGTGTCGGTATCACCAGTTTTAGGAGGCGTGCCGGTTCCAGTTTCGATGGATGTTTTGAATGGTTTGGTGTTGGATGAGAATTATGGGGTGGTGGGTGTGAAACTTATATTTCAAGGCAGGTTGAGGTGGAAAACTGGTGAGATAAAATCTGCACATTATAATATGTATGCCAAGTGTGATTTATTGTTGGGTTTGAAGAAAGGTCTTGTGGGTCAGATTCCTCTTATTGGTGCTCCAGTTTGTGATGTGGATACATAA
Protein sequence

>MS.gene82004.t1

MEDEIMHIERQEDPNQEHHSQRHFPNKMPQQQQQQRCQYPEMEQPRQQQRHQYPEMEQPRQQQRHQYPEMEQPRQQQRHQYPREHKGMVPRYRVPNAPKREHCICITIFFLVLGIIILILWLAYHPSKPHITVTSAAIYGLNATSPPDMSISMQFTIFIRNPNKRVSIYFDRLSAFVSYRNHPITPHHMLPSLYLEKHETVSVSPVLGGVPVPVSMDVLNGLVLDENYGVVGVKLIFQGRLRWKTGEIKSAHYNMYAKCDLLLGLKKGLVGQIPLIGAPVCDVDT