Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82019.t1 | XP_013444560.1 | 97.4 | 419 | 11 | 0 | 1 | 419 | 163 | 581 | 6.20E-242 | 846.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82019.t1 | O81032 | 67.7 | 421 | 135 | 1 | 1 | 420 | 172 | 592 | 7.3e-175 | 614.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82019.t1 | A0A072TYF3 | 97.4 | 419 | 11 | 0 | 1 | 419 | 163 | 581 | 4.5e-242 | 846.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060494 | MS.gene82019 | 0.827228 | 1.76E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82019.t1 | MTR_8g024560 | 97.143 | 420 | 12 | 0 | 1 | 420 | 163 | 582 | 0.0 | 855 |
| MS.gene82019.t1 | MTR_5g005540 | 68.182 | 418 | 131 | 2 | 1 | 417 | 158 | 574 | 0.0 | 586 |
| MS.gene82019.t1 | MTR_1g026710 | 50.600 | 417 | 201 | 3 | 1 | 417 | 87 | 498 | 4.21e-148 | 431 |
| MS.gene82019.t1 | MTR_7g118100 | 75.000 | 136 | 34 | 0 | 285 | 420 | 18 | 153 | 2.00e-67 | 211 |
| MS.gene82019.t1 | MTR_1g026680 | 41.636 | 269 | 125 | 2 | 67 | 335 | 58 | 294 | 1.53e-63 | 206 |
| MS.gene82019.t1 | MTR_8g024550 | 75.926 | 54 | 13 | 0 | 288 | 341 | 3 | 56 | 1.54e-26 | 101 |
| MS.gene82019.t1 | MTR_6g033475 | 70.423 | 71 | 20 | 1 | 249 | 318 | 116 | 186 | 1.11e-24 | 100 |
| MS.gene82019.t1 | MTR_1g051520 | 42.222 | 90 | 51 | 1 | 239 | 328 | 9 | 97 | 3.94e-17 | 77.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82019.t1 | AT2G46760 | 67.696 | 421 | 135 | 1 | 1 | 420 | 172 | 592 | 0.0 | 624 |
| MS.gene82019.t1 | AT2G46750 | 65.238 | 420 | 145 | 1 | 1 | 419 | 164 | 583 | 0.0 | 604 |
| MS.gene82019.t1 | AT5G56490 | 64.846 | 421 | 146 | 2 | 1 | 419 | 154 | 574 | 0.0 | 587 |
| MS.gene82019.t1 | AT2G46740 | 62.857 | 420 | 155 | 1 | 1 | 419 | 168 | 587 | 0.0 | 584 |
| MS.gene82019.t1 | AT1G32300 | 63.420 | 421 | 152 | 2 | 1 | 419 | 155 | 575 | 0.0 | 582 |
| MS.gene82019.t1 | AT5G11540 | 47.015 | 402 | 209 | 3 | 18 | 417 | 176 | 575 | 1.10e-127 | 381 |
| MS.gene82019.t1 | AT5G56470 | 61.885 | 244 | 92 | 1 | 176 | 418 | 5 | 248 | 2.95e-113 | 332 |
Find 84 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATAATCGTCTTCAATCTTC+TGG | 0.188911 | 8.2:-71292507 | MS.gene82019:CDS |
| ACCATTGCCAGATGTGTTTA+TGG | 0.199281 | 8.2:+71296404 | None:intergenic |
| TTCACCGGATACCCTATAAT+TGG | 0.248554 | 8.2:-71292534 | MS.gene82019:CDS |
| CTTGAAGAGATTGAACAAAT+TGG | 0.251791 | 8.2:-71292171 | MS.gene82019:CDS |
| GGAGATCAAGTGGTGAGTTT+TGG | 0.264261 | 8.2:-71296504 | MS.gene82019:CDS |
| CGATTATGAAATCCAATTAT+AGG | 0.307683 | 8.2:+71292522 | None:intergenic |
| GCTTGGGACTCAAGAATAAA+AGG | 0.312743 | 8.2:-71292441 | MS.gene82019:CDS |
| CTGGCTGCCATAAGAGTTAC+AGG | 0.328354 | 8.2:-71296339 | MS.gene82019:intron |
| CTACAAAAGTACCCTTTACT+TGG | 0.328388 | 8.2:+71291904 | None:intergenic |
| GCATATGGGCTTACGAATAA+TGG | 0.338429 | 8.2:-71292672 | MS.gene82019:intron |
| ATTCTCTTCAAATCTGCATA+TGG | 0.343203 | 8.2:-71292687 | MS.gene82019:CDS |
| AAGCTCACCTGTAACTCTTA+TGG | 0.386717 | 8.2:+71296332 | None:intergenic |
| AGTATGGAGGGTTACCTCAT+TGG | 0.400674 | 8.2:-71292140 | MS.gene82019:CDS |
| CCATTGCCAGATGTGTTTAT+GGG | 0.400920 | 8.2:+71296405 | None:intergenic |
| GATTATGAAATCCAATTATA+GGG | 0.406384 | 8.2:+71292523 | None:intergenic |
| AATAGAAATGTGGCATTTGA+AGG | 0.414793 | 8.2:-71292111 | MS.gene82019:CDS |
| GTATGGAGGGTTACCTCATT+GGG | 0.419078 | 8.2:-71292139 | MS.gene82019:CDS |
| TCCCACTCCTTCCATTGCAC+TGG | 0.433347 | 8.2:-71296358 | MS.gene82019:CDS |
| ATGGAAGGAGTGGGACGAAA+GGG | 0.441145 | 8.2:+71296366 | None:intergenic |
| ATTCATTGCAATCCAAGTAA+AGG | 0.454440 | 8.2:-71291916 | MS.gene82019:CDS |
| TCAATTCCACATAACCCCTT+TGG | 0.454598 | 8.2:+71292330 | None:intergenic |
| TTCATTGCAATCCAAGTAAA+GGG | 0.455588 | 8.2:-71291915 | MS.gene82019:CDS |
| TTCTCTTCAAATCTGCATAT+GGG | 0.459362 | 8.2:-71292686 | MS.gene82019:CDS |
| GCAAAGATCCAATGGCTCCT+AGG | 0.463378 | 8.2:-71292209 | MS.gene82019:CDS |
| AAATCCAATTATAGGGTATC+CGG | 0.463379 | 8.2:+71292530 | None:intergenic |
| AGACCTTCGGATTCTGAAGA+TGG | 0.464622 | 8.2:-71303383 | MS.gene82019:CDS |
| CGTGTTCCCATAAACACATC+TGG | 0.467324 | 8.2:-71296411 | MS.gene82019:CDS |
| GATAAGGATCGTTAGACCTT+CGG | 0.471053 | 8.2:-71303396 | MS.gene82019:CDS |
| GAATTCACTGGTAATGTTGA+TGG | 0.478089 | 8.2:-71292741 | MS.gene82019:CDS |
| GGAAGCACATTGTGGGGAAA+AGG | 0.479799 | 8.2:-71303446 | MS.gene82019:CDS |
| TAGATAGCTTTGTGTTGACT+TGG | 0.483878 | 8.2:+71296447 | None:intergenic |
| AAAGTATCACTTGGTGTGCT+TGG | 0.490568 | 8.2:-71303305 | MS.gene82019:CDS |
| ATAGGTGGAATGATCGGAAC+TGG | 0.496487 | 8.2:-71303476 | None:intergenic |
| CAAAGATAACAACATGTGCT+TGG | 0.497231 | 8.2:-71292458 | MS.gene82019:CDS |
| CTAGTTCAACTAGTGCCAAA+GGG | 0.498588 | 8.2:-71292345 | MS.gene82019:CDS |
| TGCTCATGGAAGCACATTGT+GGG | 0.500964 | 8.2:-71303453 | MS.gene82019:CDS |
| ATGACTATGTTGTGGAGATA+AGG | 0.501245 | 8.2:-71303412 | MS.gene82019:CDS |
| CAAAGATCCAATGGCTCCTA+GGG | 0.502388 | 8.2:-71292208 | MS.gene82019:CDS |
| TCATAAACCCTAGGAGCCAT+TGG | 0.514872 | 8.2:+71292201 | None:intergenic |
| TGTGCTTGGTGTTATTTCAC+AGG | 0.517194 | 8.2:-71303291 | MS.gene82019:intron |
| ACGGCTTCAAGTGCTTACTT+AGG | 0.518485 | 8.2:-71292276 | MS.gene82019:CDS |
| CTAGTGCCAAAGGGGTTATG+TGG | 0.518507 | 8.2:-71292336 | MS.gene82019:CDS |
| GTAATGGATCGCTTGAACAT+GGG | 0.523068 | 8.2:+71296555 | None:intergenic |
| AAAGATAACAACATGTGCTT+GGG | 0.530045 | 8.2:-71292457 | MS.gene82019:CDS |
| CAAAGTCTAATGCATCCTCT+TGG | 0.533319 | 8.2:+71292250 | None:intergenic |
| CAAGTGCTTGGAATAAAAGA+AGG | 0.533447 | 8.2:-71291997 | MS.gene82019:CDS |
| ACATGGACGGATCAAGTGCT+TGG | 0.535057 | 8.2:-71292009 | MS.gene82019:CDS |
| TGTAATGGATCGCTTGAACA+TGG | 0.535640 | 8.2:+71296554 | None:intergenic |
| AGCCAGTGCAATGGAAGGAG+TGG | 0.541636 | 8.2:+71296356 | None:intergenic |
| CAAGCGATCCATTACATATG+TGG | 0.543977 | 8.2:-71296547 | MS.gene82019:CDS |
| AATGGAAGGAGTGGGACGAA+AGG | 0.544229 | 8.2:+71296365 | None:intergenic |
| GTGGAATTGAGCAATACAAT+GGG | 0.544599 | 8.2:-71292317 | MS.gene82019:CDS |
| AGATAGCTTTGTGTTGACTT+GGG | 0.549387 | 8.2:+71296448 | None:intergenic |
| ATGATCGGAACTGGTGCTCA+TGG | 0.556298 | 8.2:-71303467 | MS.gene82019:CDS |
| TAGACCGGGCAAAATTTACA+AGG | 0.556346 | 8.2:-71291884 | MS.gene82019:CDS |
| GCTAGTTCAACTAGTGCCAA+AGG | 0.560896 | 8.2:-71292346 | MS.gene82019:CDS |
| CTTGTAGGTATAATCTTCAC+CGG | 0.563511 | 8.2:-71292549 | MS.gene82019:intron |
| AGAATATCTTCATAAACCCT+AGG | 0.565816 | 8.2:+71292192 | None:intergenic |
| GAGGATCTTGATGAATTCAC+TGG | 0.566382 | 8.2:-71292753 | MS.gene82019:CDS |
| GATTCTAATGCGTTATGTCA+CGG | 0.567564 | 8.2:-71292295 | MS.gene82019:CDS |
| GCCAGTGCAATGGAAGGAGT+GGG | 0.572301 | 8.2:+71296357 | None:intergenic |
| CCCATAAACACATCTGGCAA+TGG | 0.577105 | 8.2:-71296405 | MS.gene82019:CDS |
| AATGCAGCTAAAGTATCACT+TGG | 0.578730 | 8.2:-71303314 | MS.gene82019:CDS |
| TTCAGATTTGGGAGATCAAG+TGG | 0.578792 | 8.2:-71296514 | MS.gene82019:CDS |
| GAGCCATTGGATCTTTGCTA+CGG | 0.582368 | 8.2:+71292214 | None:intergenic |
| TTTCTATTCTTACCCCAATG+AGG | 0.589593 | 8.2:+71292126 | None:intergenic |
| TGGAAGGAGTGGGACGAAAG+GGG | 0.592595 | 8.2:+71296367 | None:intergenic |
| TTAACAATAGGTGGAATGAT+CGG | 0.595792 | 8.2:-71303482 | None:intergenic |
| TGCTGTTCATGACTATGTTG+TGG | 0.597583 | 8.2:-71303420 | MS.gene82019:CDS |
| TCTTATGGCAGCCAGTGCAA+TGG | 0.603219 | 8.2:+71296347 | None:intergenic |
| AGATAAACTAACTCTAAATG+TGG | 0.605438 | 8.2:+71292401 | None:intergenic |
| GTGCTCATGGAAGCACATTG+TGG | 0.605590 | 8.2:-71303454 | MS.gene82019:CDS |
| TGTGGAATTGAGCAATACAA+TGG | 0.611294 | 8.2:-71292318 | MS.gene82019:CDS |
| TTACCGTAGCAAAGATCCAA+TGG | 0.619712 | 8.2:-71292217 | MS.gene82019:CDS |
| ATGGCAGCCAGTGCAATGGA+AGG | 0.624939 | 8.2:+71296351 | None:intergenic |
| TTGGGGTAAGAATAGAAATG+TGG | 0.660182 | 8.2:-71292121 | MS.gene82019:CDS |
| ATGAGTTTGCAGATATATCG+TGG | 0.673607 | 8.2:-71296473 | MS.gene82019:CDS |
| TATCCATCTTCAGAATCCGA+AGG | 0.677169 | 8.2:+71303380 | None:intergenic |
| TAGTTCAACTAGTGCCAAAG+GGG | 0.680746 | 8.2:-71292344 | MS.gene82019:CDS |
| TATGGAGGGTTACCTCATTG+GGG | 0.706301 | 8.2:-71292138 | MS.gene82019:CDS |
| TGCTTACTTAGGAAACCAAG+AGG | 0.708136 | 8.2:-71292265 | MS.gene82019:CDS |
| AAAGAGAAATATGATTCACA+AGG | 0.709239 | 8.2:-71292045 | MS.gene82019:CDS |
| ACCGGGCAAAATTTACAAGG+AGG | 0.716664 | 8.2:-71291881 | MS.gene82019:CDS |
| GCTCATGGAAGCACATTGTG+GGG | 0.736799 | 8.2:-71303452 | MS.gene82019:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 71291858 | 71303489 | 71291858 | ID=MS.gene82019 |
| chr8.2 | mRNA | 71291858 | 71303489 | 71291858 | ID=MS.gene82019.t1;Parent=MS.gene82019 |
| chr8.2 | exon | 71303292 | 71303489 | 71303292 | ID=MS.gene82019.t1.exon1;Parent=MS.gene82019.t1 |
| chr8.2 | CDS | 71303292 | 71303489 | 71303292 | ID=cds.MS.gene82019.t1;Parent=MS.gene82019.t1 |
| chr8.2 | exon | 71296340 | 71296595 | 71296340 | ID=MS.gene82019.t1.exon2;Parent=MS.gene82019.t1 |
| chr8.2 | CDS | 71296340 | 71296595 | 71296340 | ID=cds.MS.gene82019.t1;Parent=MS.gene82019.t1 |
| chr8.2 | exon | 71292673 | 71292774 | 71292673 | ID=MS.gene82019.t1.exon3;Parent=MS.gene82019.t1 |
| chr8.2 | CDS | 71292673 | 71292774 | 71292673 | ID=cds.MS.gene82019.t1;Parent=MS.gene82019.t1 |
| chr8.2 | exon | 71291858 | 71292564 | 71291858 | ID=MS.gene82019.t1.exon4;Parent=MS.gene82019.t1 |
| chr8.2 | CDS | 71291858 | 71292564 | 71291858 | ID=cds.MS.gene82019.t1;Parent=MS.gene82019.t1 |
| Gene Sequence |
| Protein sequence |
