Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060494.t1 | XP_013444560.1 | 96.7 | 509 | 17 | 0 | 1 | 509 | 74 | 582 | 7.40E-290 | 1005.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060494.t1 | O81032 | 65.2 | 509 | 176 | 1 | 1 | 508 | 83 | 591 | 8.2e-205 | 714.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060494.t1 | A0A072TYF3 | 96.7 | 509 | 17 | 0 | 1 | 509 | 74 | 582 | 5.4e-290 | 1005.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060494 | MS.gene067323 | 0.800287 | 1.55E-48 | -1.69E-46 |
| MS.gene060494 | MS.gene63578 | 0.806647 | 7.43E-50 | -1.69E-46 |
| MS.gene060494 | MS.gene68228 | 0.80218 | 6.34E-49 | -1.69E-46 |
| MS.gene060494 | MS.gene76883 | 0.803998 | 2.67E-49 | -1.69E-46 |
| MS.gene060494 | MS.gene79858 | 0.803312 | 3.70E-49 | -1.69E-46 |
| MS.gene060494 | MS.gene82019 | 0.827228 | 1.76E-54 | -1.69E-46 |
| MS.gene060494 | MS.gene99127 | 0.802068 | 6.69E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060494.t1 | MTR_8g024560 | 96.660 | 509 | 17 | 0 | 1 | 509 | 74 | 582 | 0.0 | 1021 |
| MS.gene060494.t1 | MTR_5g005540 | 67.258 | 507 | 163 | 3 | 1 | 506 | 70 | 574 | 0.0 | 700 |
| MS.gene060494.t1 | MTR_1g026710 | 50.328 | 457 | 222 | 3 | 50 | 506 | 47 | 498 | 2.88e-161 | 468 |
| MS.gene060494.t1 | MTR_7g118100 | 75.556 | 135 | 33 | 0 | 374 | 508 | 18 | 152 | 1.12e-66 | 212 |
| MS.gene060494.t1 | MTR_1g026680 | 42.007 | 269 | 124 | 2 | 156 | 424 | 58 | 294 | 8.64e-63 | 207 |
| MS.gene060494.t1 | MTR_8g024550 | 75.926 | 54 | 13 | 0 | 377 | 430 | 3 | 56 | 1.83e-26 | 102 |
| MS.gene060494.t1 | MTR_6g033475 | 70.423 | 71 | 20 | 1 | 338 | 407 | 116 | 186 | 1.74e-24 | 100 |
| MS.gene060494.t1 | MTR_7g118095 | 62.500 | 72 | 25 | 1 | 1 | 70 | 75 | 146 | 1.44e-22 | 94.4 |
| MS.gene060494.t1 | MTR_1g051520 | 42.222 | 90 | 51 | 1 | 328 | 417 | 9 | 97 | 5.03e-17 | 77.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060494.t1 | AT2G46760 | 65.226 | 509 | 176 | 1 | 1 | 508 | 83 | 591 | 0.0 | 731 |
| MS.gene060494.t1 | AT2G46750 | 63.137 | 510 | 187 | 1 | 1 | 509 | 75 | 584 | 0.0 | 710 |
| MS.gene060494.t1 | AT5G56490 | 63.601 | 511 | 184 | 2 | 1 | 509 | 65 | 575 | 0.0 | 700 |
| MS.gene060494.t1 | AT2G46740 | 61.176 | 510 | 197 | 1 | 1 | 509 | 79 | 588 | 0.0 | 693 |
| MS.gene060494.t1 | AT1G32300 | 62.035 | 511 | 192 | 2 | 1 | 509 | 66 | 576 | 0.0 | 692 |
| MS.gene060494.t1 | AT5G11540 | 45.957 | 507 | 270 | 3 | 2 | 506 | 71 | 575 | 5.09e-155 | 454 |
| MS.gene060494.t1 | AT5G56470 | 62.195 | 246 | 92 | 1 | 265 | 509 | 5 | 250 | 8.11e-113 | 334 |
Find 102 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCTGGACATACCAATTT+TGG | 0.164055 | 8.1:+75775607 | None:intergenic |
| ATTCTCTTCAAATCTGCTTA+TGG | 0.182736 | 8.1:-75765426 | MS.gene060494:CDS |
| CATAACCGTCTTCAATCTTC+TGG | 0.197666 | 8.1:-75765245 | MS.gene060494:CDS |
| TTTACTGGATACCCTGTAAT+TGG | 0.202457 | 8.1:-75765272 | MS.gene060494:CDS |
| CTTGAAGAAATTGAACAAAT+TGG | 0.203853 | 8.1:-75764909 | MS.gene060494:CDS |
| AATATCCCATTTGAATCTTC+TGG | 0.225555 | 8.1:+75775592 | None:intergenic |
| CTTGTAGGTATAATCTTTAC+TGG | 0.227732 | 8.1:-75765287 | MS.gene060494:intron |
| ATTAATGAAGCCTCAAAATA+TGG | 0.248241 | 8.1:-75775478 | MS.gene060494:CDS |
| TAGATAGCTTTGTGTTGATT+TGG | 0.261808 | 8.1:+75768435 | None:intergenic |
| GGAGATCAAGTGGTGAGTTT+TGG | 0.264261 | 8.1:-75768492 | MS.gene060494:CDS |
| CCATTGCCAGATGTATTTAT+AGG | 0.266199 | 8.1:+75768393 | None:intergenic |
| CCATACCAATATGGTGTATA+TGG | 0.268864 | 8.1:+75775445 | None:intergenic |
| TTCACACAGCGTACCAAAAT+TGG | 0.274218 | 8.1:-75775620 | MS.gene060494:CDS |
| TTCTCTTCAAATCTGCTTAT+GGG | 0.292802 | 8.1:-75765425 | MS.gene060494:CDS |
| GCTTGGGACTCAAGAATAAA+AGG | 0.312743 | 8.1:-75765179 | MS.gene060494:CDS |
| CTACAAAAGTACCCTTTACT+TGG | 0.328388 | 8.1:+75764642 | None:intergenic |
| GCTTATGGGCTTACGAATAA+TGG | 0.336257 | 8.1:-75765411 | MS.gene060494:intron |
| CTTTACCATATACACCATAT+TGG | 0.340310 | 8.1:-75775450 | MS.gene060494:CDS |
| AGATAGCTTTGTGTTGATTT+GGG | 0.350795 | 8.1:+75768436 | None:intergenic |
| TATTGGTATGGTTTAACAAT+AGG | 0.351486 | 8.1:-75775433 | MS.gene060494:CDS |
| CGGTTATGAAATCCAATTAC+AGG | 0.356659 | 8.1:+75765260 | None:intergenic |
| TGAAGCCTCAAAATATGGTT+TGG | 0.389002 | 8.1:-75775473 | MS.gene060494:CDS |
| AAGCTGACCTGTGATTCTTA+TGG | 0.392062 | 8.1:+75768320 | None:intergenic |
| AGTATGGAGGGTTACCTCAT+TGG | 0.400674 | 8.1:-75764878 | MS.gene060494:CDS |
| GCACTTTCCATCAACATTAC+CGG | 0.402460 | 8.1:+75765473 | None:intergenic |
| AATAGAAATGTGGCATTTGA+AGG | 0.414793 | 8.1:-75764849 | MS.gene060494:CDS |
| GTATGGAGGGTTACCTCATT+GGG | 0.419078 | 8.1:-75764877 | MS.gene060494:CDS |
| ACGTGTAGCAACTTTGACTT+TGG | 0.419589 | 8.1:+75775645 | None:intergenic |
| TCCCACTCCTTCCATTGCAC+TGG | 0.433347 | 8.1:-75768346 | MS.gene060494:CDS |
| CGTGTTCCTATAAATACATC+TGG | 0.439311 | 8.1:-75768399 | MS.gene060494:CDS |
| ATGGAAGGAGTGGGACGAAA+GGG | 0.441145 | 8.1:+75768354 | None:intergenic |
| ATTCATTGCAATCCAAGTAA+AGG | 0.454440 | 8.1:-75764654 | MS.gene060494:CDS |
| TTCATTGCAATCCAAGTAAA+GGG | 0.455588 | 8.1:-75764653 | MS.gene060494:CDS |
| ATTGTTAAACCATACCAATA+TGG | 0.460970 | 8.1:+75775436 | None:intergenic |
| GCAAAGATCCAATGGCTCCT+AGG | 0.463378 | 8.1:-75764947 | MS.gene060494:CDS |
| AGACCTTCGGATTCTGAAGA+TGG | 0.464622 | 8.1:-75775322 | MS.gene060494:CDS |
| TCGATTCCACATAACCCCTT+TGG | 0.465105 | 8.1:+75765068 | None:intergenic |
| CAAGAACTAGTTTCAATGGT+TGG | 0.466903 | 8.1:-75775688 | None:intergenic |
| GATAAGGATCGTTAGACCTT+CGG | 0.471053 | 8.1:-75775335 | MS.gene060494:CDS |
| CCATATACACCATATTGGTA+TGG | 0.475468 | 8.1:-75775445 | MS.gene060494:CDS |
| GGAAGCACATTGTGGGGAAA+AGG | 0.479799 | 8.1:-75775385 | MS.gene060494:CDS |
| AAAGTATCACTTGGTGTGCT+TGG | 0.490568 | 8.1:-75775244 | MS.gene060494:CDS |
| ATAGGTGGAATGATCGGAAC+TGG | 0.496487 | 8.1:-75775415 | MS.gene060494:CDS |
| CAAAGATAACAACATGTGCT+TGG | 0.497231 | 8.1:-75765196 | MS.gene060494:CDS |
| CTAGTTCAACTAGTGCCAAA+GGG | 0.500050 | 8.1:-75765083 | MS.gene060494:CDS |
| TGCTCATGGAAGCACATTGT+GGG | 0.500964 | 8.1:-75775392 | MS.gene060494:CDS |
| ATGACTATGTTGTGGAGATA+AGG | 0.501245 | 8.1:-75775351 | MS.gene060494:CDS |
| CAAAGATCCAATGGCTCCTA+GGG | 0.502388 | 8.1:-75764946 | MS.gene060494:CDS |
| GTATGTCCAGAAGATTCAAA+TGG | 0.506120 | 8.1:-75775598 | MS.gene060494:CDS |
| GAATTCACCGGTAATGTTGA+TGG | 0.506657 | 8.1:-75765480 | MS.gene060494:CDS |
| TCATAAACCCTAGGAGCCAT+TGG | 0.511109 | 8.1:+75764939 | None:intergenic |
| GGAAGAAGAAACAATAGCAT+TGG | 0.515706 | 8.1:-75775527 | MS.gene060494:CDS |
| TGTGCTTGGTGTTATTTCAC+AGG | 0.517194 | 8.1:-75775230 | MS.gene060494:intron |
| CTAGTGCCAAAGGGGTTATG+TGG | 0.518507 | 8.1:-75765074 | MS.gene060494:CDS |
| TGGTATGGTTTAACAATAGG+TGG | 0.522598 | 8.1:-75775430 | MS.gene060494:CDS |
| GTAATGGATCGCTTGAACAT+GGG | 0.523068 | 8.1:+75768543 | None:intergenic |
| AAAGATAACAACATGTGCTT+GGG | 0.530045 | 8.1:-75765195 | MS.gene060494:CDS |
| CAAGTGCTTGGAATAAAAGA+AGG | 0.533447 | 8.1:-75764735 | MS.gene060494:CDS |
| ACATGGACGGATCAAGTGCT+TGG | 0.535057 | 8.1:-75764747 | MS.gene060494:CDS |
| TGTAATGGATCGCTTGAACA+TGG | 0.535640 | 8.1:+75768542 | None:intergenic |
| AGCCAGTGCAATGGAAGGAG+TGG | 0.541636 | 8.1:+75768344 | None:intergenic |
| CAAGCGATCCATTACATATG+TGG | 0.543977 | 8.1:-75768535 | MS.gene060494:CDS |
| AATGGAAGGAGTGGGACGAA+AGG | 0.544229 | 8.1:+75768353 | None:intergenic |
| CAAAGTCTAATGCATCCTCT+TGG | 0.555717 | 8.1:+75764988 | None:intergenic |
| ATGATCGGAACTGGTGCTCA+TGG | 0.556298 | 8.1:-75775406 | MS.gene060494:CDS |
| TAGACCGGGCAAAATTTACA+AGG | 0.556346 | 8.1:-75764622 | MS.gene060494:CDS |
| CTGGCTGCCATAAGAATCAC+AGG | 0.562942 | 8.1:-75768327 | MS.gene060494:intron |
| AATTCTAATGCGTTATGTGA+CGG | 0.569583 | 8.1:-75765033 | MS.gene060494:CDS |
| GCCAGTGCAATGGAAGGAGT+GGG | 0.572301 | 8.1:+75768345 | None:intergenic |
| TATGTCCAGAAGATTCAAAT+GGG | 0.573247 | 8.1:-75775597 | MS.gene060494:CDS |
| ACTAGTTCAACTAGTGCCAA+AGG | 0.575968 | 8.1:-75765084 | MS.gene060494:CDS |
| AATGCAGCTAAAGTATCACT+TGG | 0.578730 | 8.1:-75775253 | MS.gene060494:CDS |
| TTCAGATTTGGGAGATCAAG+TGG | 0.578792 | 8.1:-75768502 | MS.gene060494:CDS |
| GAGCCATTGGATCTTTGCTA+CGG | 0.582368 | 8.1:+75764952 | None:intergenic |
| TTTCTATTCTTACCCCAATG+AGG | 0.589593 | 8.1:+75764864 | None:intergenic |
| CCTATAAATACATCTGGCAA+TGG | 0.589797 | 8.1:-75768393 | MS.gene060494:CDS |
| TGGAAGGAGTGGGACGAAAG+GGG | 0.592595 | 8.1:+75768355 | None:intergenic |
| TTAACAATAGGTGGAATGAT+CGG | 0.595792 | 8.1:-75775421 | MS.gene060494:CDS |
| TGCTGTTCATGACTATGTTG+TGG | 0.597583 | 8.1:-75775359 | MS.gene060494:CDS |
| AGAATGTCTTCATAAACCCT+AGG | 0.601673 | 8.1:+75764930 | None:intergenic |
| AGATAAACTAACTCTAAATG+TGG | 0.605438 | 8.1:+75765139 | None:intergenic |
| GTGCTCATGGAAGCACATTG+TGG | 0.605590 | 8.1:-75775393 | MS.gene060494:CDS |
| TCTTATGGCAGCCAGTGCAA+TGG | 0.605702 | 8.1:+75768335 | None:intergenic |
| GAGGATCTTGATGAATTCAC+CGG | 0.609550 | 8.1:-75765492 | MS.gene060494:CDS |
| GAAACAATAGCATTGGAGAG+TGG | 0.612458 | 8.1:-75775520 | MS.gene060494:CDS |
| TGTGGAATCGAGCAATACAA+TGG | 0.614936 | 8.1:-75765056 | MS.gene060494:CDS |
| TTACCGTAGCAAAGATCCAA+TGG | 0.619712 | 8.1:-75764955 | MS.gene060494:CDS |
| ATGGCAGCCAGTGCAATGGA+AGG | 0.624939 | 8.1:+75768339 | None:intergenic |
| ACGGCTTCAAGTGCTTACTT+AGG | 0.625852 | 8.1:-75765014 | MS.gene060494:CDS |
| ATGATCCAGAAGATTGAAGA+CGG | 0.651809 | 8.1:+75765240 | None:intergenic |
| TTGGGGTAAGAATAGAAATG+TGG | 0.660182 | 8.1:-75764859 | MS.gene060494:CDS |
| AAAGAGAAATATGATTCACA+AGG | 0.660324 | 8.1:-75764783 | MS.gene060494:CDS |
| AAGAGAAATATGATTCACAA+GGG | 0.671054 | 8.1:-75764782 | MS.gene060494:CDS |
| ATGAGTTTGCAGATATATCG+TGG | 0.673607 | 8.1:-75768461 | MS.gene060494:CDS |
| TATCCATCTTCAGAATCCGA+AGG | 0.677169 | 8.1:+75775319 | None:intergenic |
| TAGTTCAACTAGTGCCAAAG+GGG | 0.684263 | 8.1:-75765082 | MS.gene060494:CDS |
| GGTTATGAAATCCAATTACA+GGG | 0.689622 | 8.1:+75765261 | None:intergenic |
| TGCTTACTTAGGCAACCAAG+AGG | 0.696185 | 8.1:-75765003 | MS.gene060494:CDS |
| TATGGAGGGTTACCTCATTG+GGG | 0.706301 | 8.1:-75764876 | MS.gene060494:CDS |
| ACCGGGCAAAATTTACAAGG+AGG | 0.716664 | 8.1:-75764619 | MS.gene060494:CDS |
| AAGCACGAAATATCTCAACA+AGG | 0.722713 | 8.1:-75775566 | MS.gene060494:CDS |
| GCTCATGGAAGCACATTGTG+GGG | 0.736799 | 8.1:-75775391 | MS.gene060494:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 75764596 | 75775695 | 75764596 | ID=MS.gene060494 |
| chr8.1 | mRNA | 75764596 | 75775695 | 75764596 | ID=MS.gene060494.t1;Parent=MS.gene060494 |
| chr8.1 | exon | 75775231 | 75775695 | 75775231 | ID=MS.gene060494.t1.exon1;Parent=MS.gene060494.t1 |
| chr8.1 | CDS | 75775231 | 75775695 | 75775231 | ID=cds.MS.gene060494.t1;Parent=MS.gene060494.t1 |
| chr8.1 | exon | 75768328 | 75768583 | 75768328 | ID=MS.gene060494.t1.exon2;Parent=MS.gene060494.t1 |
| chr8.1 | CDS | 75768328 | 75768583 | 75768328 | ID=cds.MS.gene060494.t1;Parent=MS.gene060494.t1 |
| chr8.1 | exon | 75765412 | 75765513 | 75765412 | ID=MS.gene060494.t1.exon3;Parent=MS.gene060494.t1 |
| chr8.1 | CDS | 75765412 | 75765513 | 75765412 | ID=cds.MS.gene060494.t1;Parent=MS.gene060494.t1 |
| chr8.1 | exon | 75764596 | 75765302 | 75764596 | ID=MS.gene060494.t1.exon4;Parent=MS.gene060494.t1 |
| chr8.1 | CDS | 75764596 | 75765302 | 75764596 | ID=cds.MS.gene060494.t1;Parent=MS.gene060494.t1 |
| Gene Sequence |
| Protein sequence |
