Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82031.t1 | XP_013444575.1 | 94.3 | 261 | 13 | 1 | 1 | 259 | 204 | 464 | 9.10E-127 | 463 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82031.t1 | O49687 | 42.6 | 256 | 133 | 6 | 9 | 258 | 339 | 586 | 4.9e-44 | 179.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82031.t1 | A0A072TYH1 | 94.3 | 261 | 13 | 1 | 1 | 259 | 204 | 464 | 6.6e-127 | 463.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene82031.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050657 | MS.gene82031 | 0.828543 | 8.48E-55 | -1.69E-46 |
| MS.gene060503 | MS.gene82031 | 0.905227 | 5.36E-80 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82031.t1 | MTR_8g024790 | 94.253 | 261 | 13 | 1 | 1 | 259 | 204 | 464 | 0.0 | 506 |
| MS.gene82031.t1 | MTR_8g024770 | 77.606 | 259 | 51 | 5 | 4 | 257 | 198 | 454 | 2.01e-134 | 387 |
| MS.gene82031.t1 | MTR_5g030430 | 50.990 | 202 | 88 | 4 | 67 | 258 | 474 | 674 | 9.62e-58 | 195 |
| MS.gene82031.t1 | MTR_8g067280 | 48.020 | 202 | 92 | 4 | 68 | 258 | 444 | 643 | 5.87e-54 | 184 |
| MS.gene82031.t1 | MTR_7g096350 | 40.698 | 172 | 77 | 3 | 66 | 237 | 335 | 481 | 1.06e-32 | 125 |
| MS.gene82031.t1 | MTR_8g027495 | 37.975 | 158 | 89 | 1 | 80 | 237 | 452 | 600 | 7.09e-29 | 115 |
| MS.gene82031.t1 | MTR_7g117670 | 37.342 | 158 | 90 | 1 | 80 | 237 | 396 | 544 | 5.22e-28 | 112 |
| MS.gene82031.t1 | MTR_4g097940 | 30.500 | 200 | 111 | 7 | 40 | 223 | 102 | 289 | 1.79e-18 | 83.6 |
| MS.gene82031.t1 | MTR_1g072320 | 33.526 | 173 | 103 | 3 | 78 | 238 | 457 | 629 | 2.05e-18 | 84.7 |
| MS.gene82031.t1 | MTR_4g009110 | 27.230 | 213 | 106 | 6 | 40 | 223 | 121 | 313 | 9.02e-18 | 82.0 |
| MS.gene82031.t1 | MTR_2g104490 | 29.444 | 180 | 113 | 6 | 69 | 238 | 116 | 291 | 2.10e-17 | 80.1 |
| MS.gene82031.t1 | MTR_3g104100 | 33.939 | 165 | 98 | 4 | 74 | 228 | 183 | 346 | 4.41e-17 | 80.1 |
| MS.gene82031.t1 | MTR_4g009540 | 30.435 | 161 | 95 | 5 | 72 | 223 | 142 | 294 | 1.11e-16 | 78.6 |
| MS.gene82031.t1 | MTR_2g104490 | 30.625 | 160 | 102 | 5 | 69 | 223 | 116 | 271 | 1.11e-16 | 78.6 |
| MS.gene82031.t1 | MTR_4g009540 | 30.435 | 161 | 95 | 5 | 72 | 223 | 142 | 294 | 1.29e-16 | 78.6 |
| MS.gene82031.t1 | MTR_5g014640 | 30.899 | 178 | 108 | 5 | 87 | 256 | 155 | 325 | 1.81e-16 | 77.8 |
| MS.gene82031.t1 | MTR_2g104550 | 27.329 | 161 | 109 | 4 | 69 | 223 | 117 | 275 | 1.86e-16 | 77.8 |
| MS.gene82031.t1 | MTR_0250s0040 | 28.729 | 181 | 109 | 5 | 86 | 254 | 88 | 260 | 1.93e-16 | 77.0 |
| MS.gene82031.t1 | MTR_6g047570 | 32.168 | 143 | 90 | 3 | 57 | 198 | 134 | 270 | 2.99e-16 | 77.4 |
| MS.gene82031.t1 | MTR_2g022280 | 26.136 | 176 | 116 | 4 | 85 | 248 | 1 | 174 | 1.08e-15 | 73.6 |
| MS.gene82031.t1 | MTR_0011s0260 | 31.159 | 138 | 94 | 1 | 86 | 223 | 183 | 319 | 2.30e-15 | 75.1 |
| MS.gene82031.t1 | MTR_4g066460 | 31.655 | 139 | 79 | 3 | 59 | 196 | 138 | 261 | 2.44e-15 | 74.7 |
| MS.gene82031.t1 | MTR_4g092700 | 28.261 | 184 | 112 | 5 | 60 | 236 | 137 | 307 | 2.46e-15 | 74.7 |
| MS.gene82031.t1 | MTR_1g112680 | 28.070 | 228 | 121 | 6 | 38 | 225 | 259 | 483 | 3.01e-15 | 75.5 |
| MS.gene82031.t1 | MTR_4g067010 | 26.857 | 175 | 107 | 5 | 55 | 223 | 121 | 280 | 4.68e-15 | 73.9 |
| MS.gene82031.t1 | MTR_2g081040 | 35.032 | 157 | 87 | 4 | 83 | 227 | 174 | 327 | 5.29e-15 | 73.9 |
| MS.gene82031.t1 | MTR_0011s0210 | 30.216 | 139 | 94 | 2 | 86 | 223 | 183 | 319 | 5.45e-15 | 73.9 |
| MS.gene82031.t1 | MTR_4g097920 | 28.643 | 199 | 115 | 7 | 40 | 225 | 102 | 286 | 8.47e-15 | 73.2 |
| MS.gene82031.t1 | MTR_4g097920 | 28.643 | 199 | 115 | 7 | 40 | 225 | 87 | 271 | 9.62e-15 | 72.8 |
| MS.gene82031.t1 | MTR_3g030700 | 34.118 | 170 | 94 | 5 | 69 | 229 | 183 | 343 | 1.13e-14 | 73.2 |
| MS.gene82031.t1 | MTR_5g014600 | 28.293 | 205 | 124 | 8 | 26 | 215 | 91 | 287 | 2.30e-14 | 72.0 |
| MS.gene82031.t1 | MTR_8g098275 | 38.835 | 103 | 53 | 2 | 85 | 177 | 400 | 502 | 2.70e-14 | 72.4 |
| MS.gene82031.t1 | MTR_8g098275 | 38.835 | 103 | 53 | 2 | 85 | 177 | 243 | 345 | 2.87e-14 | 72.4 |
| MS.gene82031.t1 | MTR_8g098275 | 38.835 | 103 | 53 | 2 | 85 | 177 | 307 | 409 | 2.92e-14 | 72.4 |
| MS.gene82031.t1 | MTR_7g083900 | 60.000 | 55 | 22 | 0 | 84 | 138 | 284 | 338 | 3.54e-14 | 72.0 |
| MS.gene82031.t1 | MTR_7g083900 | 60.000 | 55 | 22 | 0 | 84 | 138 | 284 | 338 | 3.65e-14 | 72.0 |
| MS.gene82031.t1 | MTR_3g498695 | 57.143 | 56 | 24 | 0 | 84 | 139 | 296 | 351 | 4.37e-14 | 72.0 |
| MS.gene82031.t1 | MTR_3g498695 | 57.143 | 56 | 24 | 0 | 84 | 139 | 296 | 351 | 4.42e-14 | 71.6 |
| MS.gene82031.t1 | MTR_4g097950 | 32.353 | 136 | 83 | 3 | 71 | 198 | 54 | 188 | 9.28e-14 | 69.3 |
| MS.gene82031.t1 | MTR_6g047550 | 37.500 | 112 | 62 | 2 | 57 | 168 | 148 | 251 | 1.26e-13 | 69.3 |
| MS.gene82031.t1 | MTR_2g010450 | 28.302 | 159 | 98 | 3 | 86 | 236 | 177 | 327 | 1.56e-13 | 69.7 |
| MS.gene82031.t1 | MTR_2g038040 | 53.226 | 62 | 22 | 1 | 87 | 141 | 53 | 114 | 1.95e-13 | 68.6 |
| MS.gene82031.t1 | MTR_4g066380 | 25.854 | 205 | 130 | 6 | 60 | 258 | 137 | 325 | 2.50e-13 | 68.9 |
| MS.gene82031.t1 | MTR_2g104530 | 28.777 | 139 | 94 | 1 | 85 | 223 | 104 | 237 | 3.31e-13 | 68.2 |
| MS.gene82031.t1 | MTR_0246s0020 | 49.254 | 67 | 30 | 1 | 86 | 152 | 198 | 260 | 3.38e-13 | 68.9 |
| MS.gene82031.t1 | MTR_0246s0020 | 49.254 | 67 | 30 | 1 | 86 | 152 | 198 | 260 | 3.44e-13 | 68.9 |
| MS.gene82031.t1 | MTR_8g009020 | 25.316 | 158 | 112 | 2 | 72 | 223 | 129 | 286 | 3.68e-12 | 65.5 |
| MS.gene82031.t1 | MTR_2g104500 | 25.806 | 186 | 130 | 5 | 38 | 223 | 58 | 235 | 6.76e-12 | 64.3 |
| MS.gene82031.t1 | MTR_4g094762 | 29.448 | 163 | 82 | 6 | 87 | 228 | 293 | 443 | 1.01e-11 | 64.7 |
| MS.gene82031.t1 | MTR_4g057270 | 30.488 | 164 | 71 | 8 | 90 | 231 | 153 | 295 | 2.21e-11 | 63.5 |
| MS.gene82031.t1 | MTR_2g084280 | 29.375 | 160 | 88 | 8 | 90 | 238 | 137 | 282 | 2.74e-11 | 63.2 |
| MS.gene82031.t1 | MTR_8g064700 | 28.477 | 151 | 84 | 4 | 91 | 227 | 249 | 389 | 6.92e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82031.t1 | AT1G32640 | 53.571 | 196 | 83 | 4 | 66 | 258 | 433 | 623 | 7.53e-59 | 197 |
| MS.gene82031.t1 | AT4G17880 | 42.578 | 256 | 133 | 6 | 9 | 258 | 339 | 586 | 1.21e-56 | 191 |
| MS.gene82031.t1 | AT5G46760 | 42.915 | 247 | 111 | 5 | 26 | 258 | 359 | 589 | 8.74e-56 | 188 |
| MS.gene82031.t1 | AT5G46830 | 50.575 | 174 | 79 | 4 | 77 | 248 | 334 | 502 | 4.60e-44 | 155 |
| MS.gene82031.t1 | AT4G00870 | 51.397 | 179 | 76 | 4 | 85 | 257 | 248 | 421 | 1.79e-41 | 147 |
| MS.gene82031.t1 | AT2G46510 | 41.714 | 175 | 82 | 3 | 66 | 240 | 376 | 530 | 1.04e-35 | 134 |
| MS.gene82031.t1 | AT4G16430 | 38.122 | 181 | 88 | 2 | 66 | 246 | 300 | 456 | 9.61e-33 | 124 |
| MS.gene82031.t1 | AT1G01260 | 34.562 | 217 | 117 | 3 | 21 | 237 | 370 | 561 | 8.03e-32 | 123 |
| MS.gene82031.t1 | AT1G01260 | 34.562 | 217 | 117 | 3 | 21 | 237 | 370 | 561 | 8.03e-32 | 123 |
| MS.gene82031.t1 | AT1G01260 | 34.562 | 217 | 117 | 3 | 21 | 237 | 370 | 561 | 8.03e-32 | 123 |
| MS.gene82031.t1 | AT1G63650 | 26.486 | 185 | 119 | 3 | 86 | 253 | 405 | 589 | 9.73e-17 | 79.7 |
| MS.gene82031.t1 | AT1G63650 | 26.486 | 185 | 119 | 3 | 86 | 253 | 405 | 589 | 9.73e-17 | 79.7 |
| MS.gene82031.t1 | AT1G63650 | 26.486 | 185 | 119 | 3 | 86 | 253 | 405 | 589 | 9.73e-17 | 79.7 |
| MS.gene82031.t1 | AT4G37850 | 31.325 | 166 | 104 | 5 | 63 | 223 | 130 | 290 | 2.74e-16 | 77.4 |
| MS.gene82031.t1 | AT4G37850 | 31.325 | 166 | 104 | 5 | 63 | 223 | 130 | 290 | 2.74e-16 | 77.4 |
| MS.gene82031.t1 | AT4G09820 | 33.588 | 131 | 64 | 2 | 85 | 214 | 362 | 470 | 1.57e-15 | 76.3 |
| MS.gene82031.t1 | AT5G57150 | 26.908 | 249 | 138 | 10 | 30 | 258 | 7 | 231 | 3.47e-15 | 73.2 |
| MS.gene82031.t1 | AT5G57150 | 27.391 | 230 | 125 | 9 | 30 | 238 | 7 | 215 | 5.30e-15 | 72.4 |
| MS.gene82031.t1 | AT1G12860 | 57.895 | 57 | 24 | 0 | 84 | 140 | 265 | 321 | 6.32e-15 | 74.3 |
| MS.gene82031.t1 | AT3G26744 | 60.000 | 55 | 22 | 0 | 84 | 138 | 305 | 359 | 6.42e-15 | 74.3 |
| MS.gene82031.t1 | AT3G26744 | 60.000 | 55 | 22 | 0 | 84 | 138 | 305 | 359 | 6.53e-15 | 74.3 |
| MS.gene82031.t1 | AT3G26744 | 60.000 | 55 | 22 | 0 | 84 | 138 | 305 | 359 | 6.53e-15 | 74.3 |
| MS.gene82031.t1 | AT3G26744 | 60.000 | 55 | 22 | 0 | 84 | 138 | 305 | 359 | 6.53e-15 | 74.3 |
| MS.gene82031.t1 | AT5G57150 | 26.957 | 230 | 126 | 9 | 30 | 238 | 7 | 215 | 6.81e-15 | 72.0 |
| MS.gene82031.t1 | AT1G12860 | 57.895 | 57 | 24 | 0 | 84 | 140 | 265 | 321 | 7.14e-15 | 74.3 |
| MS.gene82031.t1 | AT5G57150 | 36.719 | 128 | 59 | 5 | 30 | 156 | 7 | 113 | 8.39e-15 | 72.0 |
| MS.gene82031.t1 | AT5G57150 | 26.721 | 247 | 138 | 10 | 30 | 254 | 7 | 232 | 1.28e-14 | 72.0 |
| MS.gene82031.t1 | AT3G06120 | 27.869 | 183 | 111 | 4 | 85 | 246 | 1 | 183 | 2.08e-14 | 70.5 |
| MS.gene82031.t1 | AT4G29930 | 54.098 | 61 | 21 | 1 | 87 | 140 | 55 | 115 | 8.42e-14 | 69.3 |
| MS.gene82031.t1 | AT4G29930 | 54.098 | 61 | 21 | 1 | 87 | 140 | 55 | 115 | 9.69e-14 | 68.9 |
| MS.gene82031.t1 | AT4G29930 | 54.098 | 61 | 21 | 1 | 87 | 140 | 55 | 115 | 9.92e-14 | 68.9 |
| MS.gene82031.t1 | AT4G29930 | 54.098 | 61 | 21 | 1 | 87 | 140 | 55 | 115 | 1.57e-13 | 68.9 |
| MS.gene82031.t1 | AT4G29930 | 54.098 | 61 | 21 | 1 | 87 | 140 | 55 | 115 | 1.57e-13 | 68.9 |
| MS.gene82031.t1 | AT4G29930 | 54.098 | 61 | 21 | 1 | 87 | 140 | 55 | 115 | 1.91e-13 | 68.6 |
| MS.gene82031.t1 | AT4G29930 | 55.000 | 60 | 20 | 1 | 88 | 140 | 56 | 115 | 1.96e-13 | 67.4 |
| MS.gene82031.t1 | AT5G41315 | 23.684 | 190 | 123 | 3 | 86 | 253 | 441 | 630 | 9.48e-13 | 68.2 |
| MS.gene82031.t1 | AT5G41315 | 23.684 | 190 | 123 | 3 | 86 | 253 | 449 | 638 | 9.61e-13 | 68.2 |
| MS.gene82031.t1 | AT5G41315 | 23.684 | 190 | 123 | 3 | 86 | 253 | 446 | 635 | 1.03e-12 | 68.2 |
| MS.gene82031.t1 | AT1G10610 | 32.847 | 137 | 85 | 4 | 87 | 218 | 235 | 369 | 1.03e-12 | 67.8 |
| MS.gene82031.t1 | AT1G10610 | 32.847 | 137 | 85 | 4 | 87 | 218 | 265 | 399 | 1.04e-12 | 67.8 |
| MS.gene82031.t1 | AT1G10610 | 32.847 | 137 | 85 | 4 | 87 | 218 | 168 | 302 | 1.12e-12 | 67.4 |
| MS.gene82031.t1 | AT2G22760 | 28.415 | 183 | 106 | 7 | 73 | 236 | 100 | 276 | 2.29e-12 | 65.9 |
| MS.gene82031.t1 | AT2G16910 | 54.000 | 50 | 23 | 0 | 90 | 139 | 318 | 367 | 3.60e-12 | 66.2 |
| MS.gene82031.t1 | AT2G22760 | 29.697 | 165 | 96 | 6 | 73 | 223 | 100 | 258 | 4.56e-12 | 65.1 |
| MS.gene82031.t1 | AT3G24140 | 24.352 | 193 | 122 | 3 | 85 | 253 | 197 | 389 | 9.94e-12 | 64.7 |
| MS.gene82031.t1 | AT3G24140 | 24.352 | 193 | 122 | 3 | 85 | 253 | 197 | 389 | 1.13e-11 | 64.7 |
| MS.gene82031.t1 | AT4G21330 | 30.065 | 153 | 85 | 3 | 87 | 227 | 33 | 175 | 3.01e-11 | 61.6 |
| MS.gene82031.t1 | AT4G38070 | 38.095 | 84 | 49 | 1 | 55 | 138 | 67 | 147 | 5.67e-11 | 61.6 |
| MS.gene82031.t1 | AT2G22750 | 27.465 | 142 | 99 | 1 | 86 | 223 | 126 | 267 | 8.04e-11 | 61.6 |
| MS.gene82031.t1 | AT2G22750 | 27.465 | 142 | 99 | 1 | 86 | 223 | 126 | 267 | 8.32e-11 | 61.6 |
| MS.gene82031.t1 | AT2G28160 | 50.980 | 51 | 25 | 0 | 90 | 140 | 135 | 185 | 8.33e-11 | 61.6 |
| MS.gene82031.t1 | AT2G28160 | 50.980 | 51 | 25 | 0 | 90 | 140 | 135 | 185 | 8.59e-11 | 61.6 |
Find 41 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATTCATTTGTTGCAAATTT+TGG | 0.152440 | 8.4:-70246025 | None:intergenic |
| ATTGAAGGATTTGGAGTTTC+AGG | 0.165432 | 8.4:+70246577 | MS.gene82031:CDS |
| GAGATTGATGTGAAGATAAT+AGG | 0.216324 | 8.4:+70246488 | MS.gene82031:CDS |
| TTATCCATCTTTGAAACATT+TGG | 0.290544 | 8.4:-70246275 | None:intergenic |
| ATCTTAAAGCATAGAATCTA+TGG | 0.304209 | 8.4:-70246244 | None:intergenic |
| AAGATTGAAGATTTAGAATC+AGG | 0.311502 | 8.4:+70246347 | MS.gene82031:CDS |
| AATGAGTGTATTGAAGGATT+TGG | 0.327266 | 8.4:+70246568 | MS.gene82031:CDS |
| TACCGATGTCGGCGAATGTT+TGG | 0.330440 | 8.4:-70245953 | None:intergenic |
| ATTCTTGTTGGTGTTGCTAA+TGG | 0.335278 | 8.4:-70246104 | None:intergenic |
| ATCAGTGTCTTCTCCGGAAC+CGG | 0.379047 | 8.4:-70245982 | None:intergenic |
| AGAGGAAGAAAACCGTTATC+AGG | 0.381422 | 8.4:+70246164 | MS.gene82031:CDS |
| TCAGCCATTCTCATGAAATT+AGG | 0.386390 | 8.4:+70246698 | MS.gene82031:CDS |
| ATCGACTGTTAATGAAGTTA+TGG | 0.398979 | 8.4:+70246616 | MS.gene82031:CDS |
| ATCGGTATCGTCTCCGGTTC+CGG | 0.406820 | 8.4:+70245969 | MS.gene82031:CDS |
| TCTGCTTCCACATGGTTTAA+CGG | 0.415651 | 8.4:-70246197 | None:intergenic |
| AAGATTAATGAGTGTATTGA+AGG | 0.416800 | 8.4:+70246562 | MS.gene82031:CDS |
| GGTTCCAAATGTTTCAAAGA+TGG | 0.435449 | 8.4:+70246271 | MS.gene82031:CDS |
| AAAAGTGAAGATGGAAATGA+TGG | 0.445716 | 8.4:+70246388 | MS.gene82031:CDS |
| TCTTCCGCGCTCATCTCGTT+AGG | 0.463829 | 8.4:-70246662 | None:intergenic |
| TGTCTCATCAGTGTCTTCTC+CGG | 0.468594 | 8.4:-70245988 | None:intergenic |
| CCGACCTAATTTCATGAGAA+TGG | 0.497641 | 8.4:-70246702 | None:intergenic |
| GGTGTTTGAGTTCCTGATAA+CGG | 0.508977 | 8.4:-70246176 | None:intergenic |
| GCCGACATCGGTATCGTCTC+CGG | 0.512586 | 8.4:+70245963 | MS.gene82031:CDS |
| TTTAGAATCAGGGCAACAAA+AGG | 0.514365 | 8.4:+70246358 | MS.gene82031:CDS |
| TTTGTCTCTCTGCTTCCACA+TGG | 0.518910 | 8.4:-70246205 | None:intergenic |
| ATTCTATGCTTTAAGATCTG+TGG | 0.527981 | 8.4:+70246250 | MS.gene82031:CDS |
| ACACTCATTAATCTTGCACT+AGG | 0.528247 | 8.4:-70246554 | None:intergenic |
| TTCCAAACATTCGCCGACAT+CGG | 0.537863 | 8.4:+70245951 | MS.gene82031:CDS |
| ATGTGGAAGCAGAGAGACAA+AGG | 0.547229 | 8.4:+70246207 | MS.gene82031:CDS |
| TCAAAAGCATTAACATTACA+AGG | 0.560113 | 8.4:-70246467 | None:intergenic |
| AGATTGAAGATTTAGAATCA+GGG | 0.563283 | 8.4:+70246348 | MS.gene82031:CDS |
| CTTGTTGGTGTTGCTAATGG+TGG | 0.567083 | 8.4:-70246101 | None:intergenic |
| GATAATAGGAAATGATGCTA+TGG | 0.577854 | 8.4:+70246502 | MS.gene82031:CDS |
| TGCAACGGCAACTTCAACTG+TGG | 0.578390 | 8.4:+70246430 | MS.gene82031:CDS |
| AAAAGAGAACCTAAGAAGAG+AGG | 0.611347 | 8.4:+70246146 | MS.gene82031:CDS |
| TGTCGATAATAACAGTGCAA+CGG | 0.631393 | 8.4:+70246415 | MS.gene82031:CDS |
| CACTCATTAATCTTGCACTA+GGG | 0.639299 | 8.4:-70246553 | None:intergenic |
| ACCGGAGACGATACCGATGT+CGG | 0.645053 | 8.4:-70245964 | None:intergenic |
| CCATTCTCATGAAATTAGGT+CGG | 0.652747 | 8.4:+70246702 | MS.gene82031:CDS |
| TCAAACACCGTTAAACCATG+TGG | 0.685029 | 8.4:+70246190 | MS.gene82031:CDS |
| AGTTCCTAACGAGATGAGCG+CGG | 0.706023 | 8.4:+70246658 | MS.gene82031:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTCATTTGTTGCAAATTT+TGG | - | chr8.4:70246028-70246047 | None:intergenic | 20.0% |
| ! | AAGATTAATGAGTGTATTGA+AGG | + | chr8.4:70246562-70246581 | MS.gene82031:CDS | 25.0% |
| ! | ATCTTAAAGCATAGAATCTA+TGG | - | chr8.4:70246247-70246266 | None:intergenic | 25.0% |
| !! | AAGATTGAAGATTTAGAATC+AGG | + | chr8.4:70246347-70246366 | MS.gene82031:CDS | 25.0% |
| !! | AGATTGAAGATTTAGAATCA+GGG | + | chr8.4:70246348-70246367 | MS.gene82031:CDS | 25.0% |
| !!! | GAATCTTTTTCAATTCTTGT+TGG | - | chr8.4:70246119-70246138 | None:intergenic | 25.0% |
| !!! | TAACATTACAAGGTCTTTTT+TGG | - | chr8.4:70246460-70246479 | None:intergenic | 25.0% |
| !!! | TCAAAAGCATTAACATTACA+AGG | - | chr8.4:70246470-70246489 | None:intergenic | 25.0% |
| !!! | TTATCCATCTTTGAAACATT+TGG | - | chr8.4:70246278-70246297 | None:intergenic | 25.0% |
| AAAAGTGAAGATGGAAATGA+TGG | + | chr8.4:70246388-70246407 | MS.gene82031:CDS | 30.0% | |
| AATGAGTGTATTGAAGGATT+TGG | + | chr8.4:70246568-70246587 | MS.gene82031:CDS | 30.0% | |
| ATCGACTGTTAATGAAGTTA+TGG | + | chr8.4:70246616-70246635 | MS.gene82031:CDS | 30.0% | |
| GAGATTGATGTGAAGATAAT+AGG | + | chr8.4:70246488-70246507 | MS.gene82031:CDS | 30.0% | |
| GATAATAGGAAATGATGCTA+TGG | + | chr8.4:70246502-70246521 | MS.gene82031:CDS | 30.0% | |
| !! | ATTCTATGCTTTAAGATCTG+TGG | + | chr8.4:70246250-70246269 | MS.gene82031:CDS | 30.0% |
| AAAAGAGAACCTAAGAAGAG+AGG | + | chr8.4:70246146-70246165 | MS.gene82031:CDS | 35.0% | |
| ACACTCATTAATCTTGCACT+AGG | - | chr8.4:70246557-70246576 | None:intergenic | 35.0% | |
| CACTCATTAATCTTGCACTA+GGG | - | chr8.4:70246556-70246575 | None:intergenic | 35.0% | |
| CCATTCTCATGAAATTAGGT+CGG | + | chr8.4:70246702-70246721 | MS.gene82031:CDS | 35.0% | |
| GGAATCGAAAAAAGTGAAGA+TGG | + | chr8.4:70246379-70246398 | MS.gene82031:CDS | 35.0% | |
| GGTTCCAAATGTTTCAAAGA+TGG | + | chr8.4:70246271-70246290 | MS.gene82031:CDS | 35.0% | |
| TCAGCCATTCTCATGAAATT+AGG | + | chr8.4:70246698-70246717 | MS.gene82031:CDS | 35.0% | |
| TGTCGATAATAACAGTGCAA+CGG | + | chr8.4:70246415-70246434 | MS.gene82031:CDS | 35.0% | |
| ! | ATTGAAGGATTTGGAGTTTC+AGG | + | chr8.4:70246577-70246596 | MS.gene82031:CDS | 35.0% |
| ! | TTCTCTTTTATCAGATGCAG+TGG | + | chr8.4:70246301-70246320 | MS.gene82031:CDS | 35.0% |
| ! | TTTAGAATCAGGGCAACAAA+AGG | + | chr8.4:70246358-70246377 | MS.gene82031:CDS | 35.0% |
| !! | ATTCTTGTTGGTGTTGCTAA+TGG | - | chr8.4:70246107-70246126 | None:intergenic | 35.0% |
| AGAGGAAGAAAACCGTTATC+AGG | + | chr8.4:70246164-70246183 | MS.gene82031:CDS | 40.0% | |
| CCGACCTAATTTCATGAGAA+TGG | - | chr8.4:70246705-70246724 | None:intergenic | 40.0% | |
| TCAAACACCGTTAAACCATG+TGG | + | chr8.4:70246190-70246209 | MS.gene82031:CDS | 40.0% | |
| TCTGCTTCCACATGGTTTAA+CGG | - | chr8.4:70246200-70246219 | None:intergenic | 40.0% | |
| ! | GGTGTTTGAGTTCCTGATAA+CGG | - | chr8.4:70246179-70246198 | None:intergenic | 40.0% |
| ! | GGTTTTCTTCCTCTCTTCTT+AGG | - | chr8.4:70246158-70246177 | None:intergenic | 40.0% |
| ATGTGGAAGCAGAGAGACAA+AGG | + | chr8.4:70246207-70246226 | MS.gene82031:CDS | 45.0% | |
| TGTCTCATCAGTGTCTTCTC+CGG | - | chr8.4:70245991-70246010 | None:intergenic | 45.0% | |
| TTCCAAACATTCGCCGACAT+CGG | + | chr8.4:70245951-70245970 | MS.gene82031:CDS | 45.0% | |
| TTTGTCTCTCTGCTTCCACA+TGG | - | chr8.4:70246208-70246227 | None:intergenic | 45.0% | |
| !! | CTTGTTGGTGTTGCTAATGG+TGG | - | chr8.4:70246104-70246123 | None:intergenic | 45.0% |
| AGTTCCTAACGAGATGAGCG+CGG | + | chr8.4:70246658-70246677 | MS.gene82031:CDS | 50.0% | |
| ATCAGTGTCTTCTCCGGAAC+CGG | - | chr8.4:70245985-70246004 | None:intergenic | 50.0% | |
| TACCGATGTCGGCGAATGTT+TGG | - | chr8.4:70245956-70245975 | None:intergenic | 50.0% | |
| TGCAACGGCAACTTCAACTG+TGG | + | chr8.4:70246430-70246449 | MS.gene82031:CDS | 50.0% | |
| ACCGGAGACGATACCGATGT+CGG | - | chr8.4:70245967-70245986 | None:intergenic | 55.0% | |
| ATCGGTATCGTCTCCGGTTC+CGG | + | chr8.4:70245969-70245988 | MS.gene82031:CDS | 55.0% | |
| TCTTCCGCGCTCATCTCGTT+AGG | - | chr8.4:70246665-70246684 | None:intergenic | 55.0% | |
| GCCGACATCGGTATCGTCTC+CGG | + | chr8.4:70245963-70245982 | MS.gene82031:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 70245948 | 70246727 | 70245948 | ID=MS.gene82031 |
| chr8.4 | mRNA | 70245948 | 70246727 | 70245948 | ID=MS.gene82031.t1;Parent=MS.gene82031 |
| chr8.4 | exon | 70245948 | 70246727 | 70245948 | ID=MS.gene82031.t1.exon1;Parent=MS.gene82031.t1 |
| chr8.4 | CDS | 70245948 | 70246727 | 70245948 | ID=cds.MS.gene82031.t1;Parent=MS.gene82031.t1 |
| Gene Sequence |
| Protein sequence |
