Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82062.t1 | XP_027188904.1 | 67.6 | 111 | 34 | 2 | 5 | 115 | 179 | 287 | 1.70E-29 | 139 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82062.t1 | A0A3Q7X5E5 | 67.6 | 111 | 34 | 2 | 5 | 115 | 179 | 287 | 1.2e-29 | 139.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05894 | MS.gene82062 | 0.831699 | 1.44E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82062.t1 | MTR_0116s0090 | 61.207 | 116 | 43 | 2 | 5 | 120 | 134 | 247 | 2.45e-39 | 133 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCAAAGAAATTGAAAATTT+TGG | 0.051328 | 7.3:+59417851 | None:intergenic |
| CCTTGTACTTAACTGTTAAA+TGG | 0.112626 | 7.3:+59417768 | None:intergenic |
| AGTAACCACCAAGCCATTAT+TGG | 0.262755 | 7.3:+59417814 | None:intergenic |
| AAGATAGAGTCTTCAAATTC+AGG | 0.329055 | 7.3:+59417926 | None:intergenic |
| GATCTAGCAAATTCCAATAA+TGG | 0.350921 | 7.3:-59417827 | MS.gene82062:CDS |
| TGTGTGACATGAAGTTTAAT+TGG | 0.394338 | 7.3:-59417877 | MS.gene82062:CDS |
| TGAATTTGAAGACTCTATCT+TGG | 0.404281 | 7.3:-59417924 | MS.gene82062:CDS |
| CTTGTACTTAACTGTTAAAT+GGG | 0.427882 | 7.3:+59417769 | None:intergenic |
| TTTGTGTTTAGCTCTAATAC+AGG | 0.448926 | 7.3:+59417692 | None:intergenic |
| AGCAAATTCCAATAATGGCT+TGG | 0.489888 | 7.3:-59417822 | MS.gene82062:CDS |
| AAAGCTTCAACTTGTGAAGA+TGG | 0.495233 | 7.3:-59417606 | MS.gene82062:CDS |
| AGATAGAGTCTTCAAATTCA+GGG | 0.508885 | 7.3:+59417927 | None:intergenic |
| CCATTTAACAGTTAAGTACA+AGG | 0.515145 | 7.3:-59417768 | MS.gene82062:CDS |
| TAATCAGAGTTCAGTACAAT+CGG | 0.518306 | 7.3:-59417639 | MS.gene82062:CDS |
| CACAGCTGACACAGAACTCT+CGG | 0.524832 | 7.3:+59417568 | None:intergenic |
| CTCTCAAACTTATTGACACA+GGG | 0.544383 | 7.3:+59417960 | None:intergenic |
| AAATTCCAATAATGGCTTGG+TGG | 0.556308 | 7.3:-59417819 | MS.gene82062:CDS |
| TGTGAAGATGGAAAAGGTGA+TGG | 0.556478 | 7.3:-59417594 | MS.gene82062:CDS |
| TCAACTTGTGAAGATGGAAA+AGG | 0.558493 | 7.3:-59417600 | MS.gene82062:CDS |
| CGAGAGTTCTGTGTCAGCTG+TGG | 0.574851 | 7.3:-59417567 | MS.gene82062:CDS |
| TCTCTCAAACTTATTGACAC+AGG | 0.599143 | 7.3:+59417959 | None:intergenic |
| TAAATGGGTAGAAGAATCTG+AGG | 0.614354 | 7.3:+59417784 | None:intergenic |
| TCAGAGTTCAGTACAATCGG+TGG | 0.645978 | 7.3:-59417636 | MS.gene82062:CDS |
| AAATGGGTAGAAGAATCTGA+GGG | 0.683935 | 7.3:+59417785 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CCAAAGAAATTGAAAATTTT+GGG | + | chr7.3:59417690-59417709 | None:intergenic | 20.0% |
| !!! | GATTTTCAAATAGATAATCA+GGG | + | chr7.3:59417828-59417847 | None:intergenic | 20.0% |
| ! | CTTGTACTTAACTGTTAAAT+GGG | + | chr7.3:59417773-59417792 | None:intergenic | 25.0% |
| !! | CCCAAAATTTTCAATTTCTT+TGG | - | chr7.3:59417687-59417706 | MS.gene82062:CDS | 25.0% |
| !! | GCCAAAGAAATTGAAAATTT+TGG | + | chr7.3:59417691-59417710 | None:intergenic | 25.0% |
| !! | GGATTTTCAAATAGATAATC+AGG | + | chr7.3:59417829-59417848 | None:intergenic | 25.0% |
| AAGATAGAGTCTTCAAATTC+AGG | + | chr7.3:59417616-59417635 | None:intergenic | 30.0% | |
| AGATAGAGTCTTCAAATTCA+GGG | + | chr7.3:59417615-59417634 | None:intergenic | 30.0% | |
| CCATTTAACAGTTAAGTACA+AGG | - | chr7.3:59417771-59417790 | MS.gene82062:CDS | 30.0% | |
| CCTTGTACTTAACTGTTAAA+TGG | + | chr7.3:59417774-59417793 | None:intergenic | 30.0% | |
| GATCTAGCAAATTCCAATAA+TGG | - | chr7.3:59417712-59417731 | MS.gene82062:CDS | 30.0% | |
| TAATCAGAGTTCAGTACAAT+CGG | - | chr7.3:59417900-59417919 | MS.gene82062:CDS | 30.0% | |
| TGAATTTGAAGACTCTATCT+TGG | - | chr7.3:59417615-59417634 | MS.gene82062:CDS | 30.0% | |
| TGTGTGACATGAAGTTTAAT+TGG | - | chr7.3:59417662-59417681 | MS.gene82062:CDS | 30.0% | |
| !! | TTTGTGTTTAGCTCTAATAC+AGG | + | chr7.3:59417850-59417869 | None:intergenic | 30.0% |
| AAAGCTTCAACTTGTGAAGA+TGG | - | chr7.3:59417933-59417952 | MS.gene82062:CDS | 35.0% | |
| AAATGGGTAGAAGAATCTGA+GGG | + | chr7.3:59417757-59417776 | None:intergenic | 35.0% | |
| AGCAAATTCCAATAATGGCT+TGG | - | chr7.3:59417717-59417736 | MS.gene82062:CDS | 35.0% | |
| CTCTCAAACTTATTGACACA+GGG | + | chr7.3:59417582-59417601 | None:intergenic | 35.0% | |
| TAAATGGGTAGAAGAATCTG+AGG | + | chr7.3:59417758-59417777 | None:intergenic | 35.0% | |
| TCAACTTGTGAAGATGGAAA+AGG | - | chr7.3:59417939-59417958 | MS.gene82062:CDS | 35.0% | |
| TCTCTCAAACTTATTGACAC+AGG | + | chr7.3:59417583-59417602 | None:intergenic | 35.0% | |
| ! | AAATTCCAATAATGGCTTGG+TGG | - | chr7.3:59417720-59417739 | MS.gene82062:CDS | 35.0% |
| AGTAACCACCAAGCCATTAT+TGG | + | chr7.3:59417728-59417747 | None:intergenic | 40.0% | |
| TGTGAAGATGGAAAAGGTGA+TGG | - | chr7.3:59417945-59417964 | MS.gene82062:CDS | 40.0% | |
| TCAGAGTTCAGTACAATCGG+TGG | - | chr7.3:59417903-59417922 | MS.gene82062:CDS | 45.0% | |
| CACAGCTGACACAGAACTCT+CGG | + | chr7.3:59417974-59417993 | None:intergenic | 50.0% | |
| CGAGAGTTCTGTGTCAGCTG+TGG | - | chr7.3:59417972-59417991 | MS.gene82062:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 59417562 | 59417999 | 59417562 | ID=MS.gene82062 |
| chr7.3 | mRNA | 59417562 | 59417999 | 59417562 | ID=MS.gene82062.t1;Parent=MS.gene82062 |
| chr7.3 | exon | 59417562 | 59417999 | 59417562 | ID=MS.gene82062.t1.exon1;Parent=MS.gene82062.t1 |
| chr7.3 | CDS | 59417562 | 59417999 | 59417562 | ID=cds.MS.gene82062.t1;Parent=MS.gene82062.t1 |
| Gene Sequence |
| Protein sequence |
