AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82240.t1 MTR_4g029040 91.163 215 19 0 1 215 1 215 1.72e-146 406
MS.gene82240.t1 MTR_7g072560 49.107 224 102 2 1 212 1 224 1.91e-79 238
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82240.t1 AT3G51730 42.453 212 117 2 1 207 1 212 3.11e-58 183
MS.gene82240.t1 AT5G01800 39.153 189 103 3 16 195 15 200 7.84e-52 167

Find 38 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATACCGTTGCGGAATTAT+TGG 0.223574 4.2:+74958088 MS.gene82240:CDS
CTTCTGTGATTGCAGCTTCC+TGG 0.224979 4.2:-74959241 None:intergenic
GAGATTATGGAAAGCAAAAT+AGG 0.235192 4.2:+74955990 None:intergenic
ACTAAAGGCATGCAATAATA+TGG 0.295458 4.2:+74958310 MS.gene82240:CDS
TGCATATATCTGCTGTCTTC+AGG 0.324490 4.2:-74959181 None:intergenic
AAATCACTAACCTTAGTTTC+AGG 0.327947 4.2:-74958125 None:intergenic
GTTGAATGATCCTGAAACTA+AGG 0.370785 4.2:+74958115 MS.gene82240:CDS
AAGATTTCTTCACAGGTTCA+TGG 0.418003 4.2:+74958044 MS.gene82240:CDS
TCACAAATTGAACATGCATC+TGG 0.423663 4.2:-74957367 None:intergenic
TTCTTCCACTATTGTTAGCC+AGG 0.431054 4.2:+74959223 MS.gene82240:CDS
GTCTGCAAAGATTTCTTCAC+AGG 0.444344 4.2:+74958037 MS.gene82240:CDS
AGATTATGGAAAGCAAAATA+GGG 0.475417 4.2:+74955991 None:intergenic
AAAATAGCTCTAATGAAGCC+TGG 0.494193 4.2:+74957984 MS.gene82240:CDS
AACAATAGTGGAAGAAGGGC+AGG 0.499361 4.2:-74959216 None:intergenic
TCTTCCGTCTTTCAGCGAGC+AGG 0.501700 4.2:+74957477 MS.gene82240:CDS
CATTGTACAGTGCTTAGAAT+TGG 0.503629 4.2:+74957932 MS.gene82240:intron
TGTACAGTGCTTAGAATTGG+TGG 0.507359 4.2:+74957935 MS.gene82240:intron
AGCTTCCTGGCTAACAATAG+TGG 0.513011 4.2:-74959228 None:intergenic
TCTGCATTGACTAGAATCAA+TGG 0.518668 4.2:-74959152 None:intergenic
TGAGATACTTGATTATCTAA+AGG 0.525760 4.2:+74957402 MS.gene82240:CDS
TTGTTTATTTACAGTGCAAG+AGG 0.527287 4.2:+74959111 MS.gene82240:intron
TGGCTAACAATAGTGGAAGA+AGG 0.530354 4.2:-74959221 None:intergenic
TAATATGGAGAAGTATAAGA+AGG 0.539882 4.2:+74958325 MS.gene82240:CDS
GATAATTCAGAAACTACTAA+AGG 0.545272 4.2:+74958295 MS.gene82240:CDS
GGCTAACAATAGTGGAAGAA+GGG 0.565538 4.2:-74959220 None:intergenic
CAGGTTCATGGAAATAGTTG+TGG 0.583323 4.2:+74958056 MS.gene82240:CDS
TCTTTGCAGACTCGCAAAGG+TGG 0.584711 4.2:-74958025 None:intergenic
GCAAGAGAGTTTGCAAACCC+TGG 0.584783 4.2:+74956056 MS.gene82240:CDS
AAATCTTTGCAGACTCGCAA+AGG 0.587749 4.2:-74958028 None:intergenic
TTATTTACAGTGCAAGAGGA+TGG 0.587964 4.2:+74959115 MS.gene82240:intron
TCTAAAGGATAACAAGACTC+AGG 0.588548 4.2:+74957417 MS.gene82240:CDS
TATGGAGAAGTATAAGAAGG+AGG 0.591525 4.2:+74958328 MS.gene82240:CDS
AGATGCATGTTCAATTTGTG+AGG 0.595452 4.2:+74957369 MS.gene82240:CDS
CAAACCTGCTCGCTGAAAGA+CGG 0.602257 4.2:-74957481 None:intergenic
CGCTGAAAGACGGAAGATGA+CGG 0.614204 4.2:-74957471 None:intergenic
CTCAACCAATAATTCCGCAA+CGG 0.648292 4.2:-74958093 None:intergenic
GATGACGGCAAGTATTGTGA+AGG 0.651523 4.2:-74957456 None:intergenic
GATTAAGAGTCAGAAAACAA+TGG 0.672126 4.2:-74959269 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATGACATTTAATAATAAA+AGG - chr4.2:74958404-74958423 None:intergenic 10.0%
!! AATGACATTTAATAATAAAA+GGG - chr4.2:74958403-74958422 None:intergenic 10.0%
!! AAATGTACATATGATATTAT+AGG - chr4.2:74957237-74957256 None:intergenic 15.0%
!! ATAAACAAGAAAATAATTGT+TGG + chr4.2:74956217-74956236 MS.gene82240:intron 15.0%
!! ATCTATACCTATATATAAAA+GGG + chr4.2:74957611-74957630 MS.gene82240:intron 15.0%
!! TAAAAAAAAGAGAAAACAAA+AGG - chr4.2:74958372-74958391 None:intergenic 15.0%
!! TATCTATACCTATATATAAA+AGG + chr4.2:74957610-74957629 MS.gene82240:intron 15.0%
!! TGTTTCAATTCAAAAATATT+TGG - chr4.2:74956859-74956878 None:intergenic 15.0%
!!! AATTAATTTTATGTAACCAA+AGG - chr4.2:74958437-74958456 None:intergenic 15.0%
!!! TCAAAAATATTTGGATTTTT+TGG - chr4.2:74956850-74956869 None:intergenic 15.0%
!! AAAATAACTACTTTCTGAAT+TGG + chr4.2:74958207-74958226 MS.gene82240:intron 20.0%
!! AAAATATGCAACAAATAAAC+CGG - chr4.2:74958933-74958952 None:intergenic 20.0%
!! AATTCATACATCTACATTAT+AGG - chr4.2:74957173-74957192 None:intergenic 20.0%
!! ATATATGTAACTGCAAAAAT+TGG - chr4.2:74959022-74959041 None:intergenic 20.0%
!! ATTTATGTAATCTTAGAAGA+TGG + chr4.2:74956152-74956171 MS.gene82240:intron 20.0%
!! GTTTAAATGAAGAGTAAAAT+CGG - chr4.2:74957668-74957687 None:intergenic 20.0%
!! TGTAAAGTACAAAGATTAAT+AGG + chr4.2:74956175-74956194 MS.gene82240:intron 20.0%
!! TTGGTCAATGTTATAAAAAA+CGG - chr4.2:74959003-74959022 None:intergenic 20.0%
!!! AAATAAAACAATTACTGACT+AGG - chr4.2:74958515-74958534 None:intergenic 20.0%
!!! AATTTTTGCAGTTACATATA+TGG + chr4.2:74959021-74959040 MS.gene82240:intron 20.0%
!!! ATATGCAATTTTCATTAGTA+AGG + chr4.2:74956742-74956761 MS.gene82240:intron 20.0%
!!! ATTGATTACAATGATTTTTC+TGG + chr4.2:74958473-74958492 MS.gene82240:intron 20.0%
!!! ATTTTCTTGTTTATCATTGA+TGG - chr4.2:74956211-74956230 None:intergenic 20.0%
!!! CTTTTATTTCTGATTTTGTT+GGG + chr4.2:74956014-74956033 MS.gene82240:CDS 20.0%
! AAATAAACTTCAAACCATCT+AGG - chr4.2:74956132-74956151 None:intergenic 25.0%
! AAGATAAACCTATTGAGAAA+AGG + chr4.2:74956812-74956831 MS.gene82240:intron 25.0%
! ACAGAGAAGAGTTAAAAATA+AGG - chr4.2:74957897-74957916 None:intergenic 25.0%
! AGTAAAATCGGAAGAAAAAA+TGG - chr4.2:74957656-74957675 None:intergenic 25.0%
! CAAGCGTTTGTATTATAAAT+TGG + chr4.2:74958714-74958733 MS.gene82240:intron 25.0%
! GATAATTCAGAAACTACTAA+AGG + chr4.2:74958295-74958314 MS.gene82240:CDS 25.0%
! TAACATAAGAAGTTGAGTTA+GGG + chr4.2:74956382-74956401 MS.gene82240:intron 25.0%
! TATCAAATATCAACCATAGA+AGG - chr4.2:74957091-74957110 None:intergenic 25.0%
! TATTAAGGAAGGATAATGTA+CGG - chr4.2:74958156-74958175 None:intergenic 25.0%
! TGAGATACTTGATTATCTAA+AGG + chr4.2:74957402-74957421 MS.gene82240:CDS 25.0%
! TTAACATAAGAAGTTGAGTT+AGG + chr4.2:74956381-74956400 MS.gene82240:intron 25.0%
! TTAGATAGAATGAGTTTGAA+TGG - chr4.2:74956464-74956483 None:intergenic 25.0%
! TTCTGATTGTATGATATATC+AGG + chr4.2:74956995-74957014 MS.gene82240:intron 25.0%
!! ATTACAATGATTTTTCTGGA+AGG + chr4.2:74958477-74958496 MS.gene82240:intron 25.0%
!! GAAGTTAACAAATTTTGCAT+TGG - chr4.2:74957144-74957163 None:intergenic 25.0%
!! TAATATGGAGAAGTATAAGA+AGG + chr4.2:74958325-74958344 MS.gene82240:CDS 25.0%
!! TCTATGGTTGATATTTGATA+TGG + chr4.2:74957091-74957110 MS.gene82240:intron 25.0%
!! TTTAAACCAGTGTTGTTAAA+TGG + chr4.2:74957681-74957700 MS.gene82240:intron 25.0%
!!! AAGCTACACTTTTTTTTTGA+AGG - chr4.2:74956946-74956965 None:intergenic 25.0%
!!! CTTTTTTTTTGAAGGAAAAC+CGG - chr4.2:74956938-74956957 None:intergenic 25.0%
!!! GCTATTTTTGAGAAGAAAAT+CGG - chr4.2:74957972-74957991 None:intergenic 25.0%
!!! GCTTTTATTTCTGATTTTGT+TGG + chr4.2:74956013-74956032 MS.gene82240:CDS 25.0%
AAATCACTAACCTTAGTTTC+AGG - chr4.2:74958128-74958147 None:intergenic 30.0%
AAATTAGTCCAAATTACGCT+CGG + chr4.2:74956679-74956698 MS.gene82240:intron 30.0%
AACAAAATAGAATCAAGCGA+AGG - chr4.2:74957510-74957529 None:intergenic 30.0%
AAGCTATTCTTGATAGATCA+CGG - chr4.2:74958625-74958644 None:intergenic 30.0%
ACATCTACATTATAGGCTAA+GGG - chr4.2:74957166-74957185 None:intergenic 30.0%
AGCGTAATTTGGACTAATTT+AGG - chr4.2:74956679-74956698 None:intergenic 30.0%
ATAGCTTACGAAAGTAAACT+AGG + chr4.2:74958638-74958657 MS.gene82240:intron 30.0%
ATTTGCAAATTACACATGAG+AGG - chr4.2:74956417-74956436 None:intergenic 30.0%
CATAAGAACAGGTAACATAT+CGG - chr4.2:74956893-74956912 None:intergenic 30.0%
CATTATCCTTCCTTAATATG+AGG + chr4.2:74958158-74958177 MS.gene82240:intron 30.0%
GCATTCTAAACTAGCATATA+GGG + chr4.2:74956239-74956258 MS.gene82240:intron 30.0%
GCTTAGGCCAATAATATATA+GGG + chr4.2:74956347-74956366 MS.gene82240:intron 30.0%
GTATTCAATTGTTCCTTCTA+TGG + chr4.2:74957075-74957094 MS.gene82240:intron 30.0%
GTTGCAATGACATGTTTATA+TGG + chr4.2:74957779-74957798 MS.gene82240:intron 30.0%
TAAATTGATGTTGTCGATGT+CGG - chr4.2:74957827-74957846 None:intergenic 30.0%
TAACCAATTAAACCATGACT+CGG + chr4.2:74958952-74958971 MS.gene82240:intron 30.0%
TACATCTACATTATAGGCTA+AGG - chr4.2:74957167-74957186 None:intergenic 30.0%
TATAGTGTGTAGATTTGTCA+AGG + chr4.2:74957854-74957873 MS.gene82240:intron 30.0%
TTGTTTATTTACAGTGCAAG+AGG + chr4.2:74959111-74959130 MS.gene82240:intron 30.0%
TTTCTCGCCCTATATATTAT+TGG - chr4.2:74956357-74956376 None:intergenic 30.0%
TTTGCAAATTACACATGAGA+GGG - chr4.2:74956416-74956435 None:intergenic 30.0%
! AAACAATTACTGACTAGGTA+AGG - chr4.2:74958510-74958529 None:intergenic 30.0%
! ACTAAAGGCATGCAATAATA+TGG + chr4.2:74958310-74958329 MS.gene82240:CDS 30.0%
! ATATGGTTCTGTTCATTTGA+TGG + chr4.2:74957108-74957127 MS.gene82240:intron 30.0%
! TATGGTTCTGTTCATTTGAT+GGG + chr4.2:74957109-74957128 MS.gene82240:intron 30.0%
! TGGACTAATTTAGGTTCTAA+GGG - chr4.2:74956670-74956689 None:intergenic 30.0%
! TTCGGTCAGATTTAAAACAT+TGG - chr4.2:74958813-74958832 None:intergenic 30.0%
! TTGGACTAATTTAGGTTCTA+AGG - chr4.2:74956671-74956690 None:intergenic 30.0%
! TTGGCATCCCTTTTATATAT+AGG - chr4.2:74957621-74957640 None:intergenic 30.0%
! TTTTCTCCATCACATTCTTT+TGG - chr4.2:74956790-74956809 None:intergenic 30.0%
!! ATTTTAATCCGAGCGTAATT+TGG - chr4.2:74956690-74956709 None:intergenic 30.0%
!! TCTTTTCTTTTTTGCCTAGA+TGG + chr4.2:74956115-74956134 MS.gene82240:intron 30.0%
!! TTTTGGCTACTTCACTTATT+TGG - chr4.2:74956773-74956792 None:intergenic 30.0%
!!! TCGACTTTTTTTAGTATAGC+CGG + chr4.2:74956916-74956935 MS.gene82240:intron 30.0%
AAAATGGCTACACCAAAACT+TGG - chr4.2:74957640-74957659 None:intergenic 35.0%
AAACATGTCATTGCAACCTA+TGG - chr4.2:74957776-74957795 None:intergenic 35.0%
AAACGGATAAGAGATCAAAC+CGG - chr4.2:74958986-74959005 None:intergenic 35.0%
AAAGTCGACTACATAAGAAC+AGG - chr4.2:74956904-74956923 None:intergenic 35.0%
AACCAATTAAACCATGACTC+GGG + chr4.2:74958953-74958972 MS.gene82240:intron 35.0%
AAGATTTCTTCACAGGTTCA+TGG + chr4.2:74958044-74958063 MS.gene82240:CDS 35.0%
AAGTAGCCAAAAGAATGTGA+TGG + chr4.2:74956781-74956800 MS.gene82240:intron 35.0%
AATGCTCCTCATATTAAGGA+AGG - chr4.2:74958167-74958186 None:intergenic 35.0%
ACAGAATCCAGGTTATATAC+AGG - chr4.2:74956975-74956994 None:intergenic 35.0%
ACCCTGGTAATTAACAATGT+TGG + chr4.2:74956072-74956091 MS.gene82240:intron 35.0%
AGATGCATGTTCAATTTGTG+AGG + chr4.2:74957369-74957388 MS.gene82240:CDS 35.0%
AGCCAACATTGTTAATTACC+AGG - chr4.2:74956077-74956096 None:intergenic 35.0%
AGGTAACATATCGGTGAAAA+GGG - chr4.2:74956884-74956903 None:intergenic 35.0%
ATACAAACCAGCGAATTTAC+TGG - chr4.2:74958836-74958855 None:intergenic 35.0%
ATTTCGCCATTTAACAACAC+TGG - chr4.2:74957690-74957709 None:intergenic 35.0%
CAAACGCTTGTGCAAATATT+AGG - chr4.2:74958704-74958723 None:intergenic 35.0%
CAATTGAATACATGAACCTC+TGG - chr4.2:74957066-74957085 None:intergenic 35.0%
CAGAATCCAGGTTATATACA+GGG - chr4.2:74956974-74956993 None:intergenic 35.0%
CATTGTACAGTGCTTAGAAT+TGG + chr4.2:74957932-74957951 MS.gene82240:intron 35.0%
CGCTTAGGCCAATAATATAT+AGG + chr4.2:74956346-74956365 MS.gene82240:intron 35.0%
CTCTAATGCTCCTCATATTA+AGG - chr4.2:74958171-74958190 None:intergenic 35.0%
CTGTTTCCAAGATTTAAACC+TGG + chr4.2:74956597-74956616 MS.gene82240:intron 35.0%
GCCAACATTGTTAATTACCA+GGG - chr4.2:74956076-74956095 None:intergenic 35.0%
GGCATTCTAAACTAGCATAT+AGG + chr4.2:74956238-74956257 MS.gene82240:intron 35.0%
GTTGAATGATCCTGAAACTA+AGG + chr4.2:74958115-74958134 MS.gene82240:CDS 35.0%
TACAATCAGAAACAGAATCC+AGG - chr4.2:74956986-74957005 None:intergenic 35.0%
TCAAGGAATGAGAACTTATC+AGG + chr4.2:74957871-74957890 MS.gene82240:intron 35.0%
TCACAAATTGAACATGCATC+TGG - chr4.2:74957370-74957389 None:intergenic 35.0%
TCTAAAGGATAACAAGACTC+AGG + chr4.2:74957417-74957436 MS.gene82240:CDS 35.0%
TCTAAGCAACTCAATCAACT+AGG - chr4.2:74957036-74957055 None:intergenic 35.0%
TCTGTTGAACTGGATTGAAT+CGG + chr4.2:74958876-74958895 MS.gene82240:intron 35.0%
TTATTTACAGTGCAAGAGGA+TGG + chr4.2:74959115-74959134 MS.gene82240:intron 35.0%
TTCTAAACTAGCATATAGGG+CGG + chr4.2:74956242-74956261 MS.gene82240:intron 35.0%
TTGGAAACAGTCTCTTTGTT+TGG - chr4.2:74956587-74956606 None:intergenic 35.0%
! ATAAAAGGGATGCCAAGTTT+TGG + chr4.2:74957625-74957644 MS.gene82240:intron 35.0%
! TAGCACAATGGTAAGGTTTT+TGG - chr4.2:74956640-74956659 None:intergenic 35.0%
! TATGGAGAAGTATAAGAAGG+AGG + chr4.2:74958328-74958347 MS.gene82240:CDS 35.0%
! TCAGAATGCCTTTTCTCAAT+AGG - chr4.2:74956823-74956842 None:intergenic 35.0%
! TCATTTTGCATGAGATCCTT+TGG + chr4.2:74958418-74958437 MS.gene82240:intron 35.0%
! TTGCTTAGATTGATTTCCAG+AGG + chr4.2:74957047-74957066 MS.gene82240:intron 35.0%
!! AAAATAGCTCTAATGAAGCC+TGG + chr4.2:74957984-74958003 MS.gene82240:CDS 35.0%
!! ACTAATTTAGGTTCTAAGGG+TGG - chr4.2:74956667-74956686 None:intergenic 35.0%
!! AGGCGAAAGTACTTAAGAAA+TGG - chr4.2:74957217-74957236 None:intergenic 35.0%
!! AGTAAACTAGGATCGACTTT+TGG + chr4.2:74958650-74958669 MS.gene82240:intron 35.0%
!! GGCGAAAGTACTTAAGAAAT+GGG - chr4.2:74957216-74957235 None:intergenic 35.0%
!! TCTGCATTGACTAGAATCAA+TGG - chr4.2:74959155-74959174 None:intergenic 35.0%
!! TTGAGAAAAGGCATTCTGAA+AGG + chr4.2:74956824-74956843 MS.gene82240:intron 35.0%
AAATCTTTGCAGACTCGCAA+AGG - chr4.2:74958031-74958050 None:intergenic 40.0%
AGATACCGTTGCGGAATTAT+TGG + chr4.2:74958088-74958107 MS.gene82240:CDS 40.0%
ATCTTCTTACCATTGCGCTT+AGG + chr4.2:74956331-74956350 MS.gene82240:intron 40.0%
CAATCAGAGAGATTGTGTCT+AGG + chr4.2:74956304-74956323 MS.gene82240:intron 40.0%
CAGGTAACATATCGGTGAAA+AGG - chr4.2:74956885-74956904 None:intergenic 40.0%
CAGGTTCATGGAAATAGTTG+TGG + chr4.2:74958056-74958075 MS.gene82240:CDS 40.0%
CTAGACACAATCTCTCTGAT+TGG - chr4.2:74956306-74956325 None:intergenic 40.0%
CTCAACCAATAATTCCGCAA+CGG - chr4.2:74958096-74958115 None:intergenic 40.0%
CTGACATAGGCAATTTGTGA+AGG + chr4.2:74957731-74957750 MS.gene82240:intron 40.0%
CTGTTGAACTGGATTGAATC+GGG + chr4.2:74958877-74958896 MS.gene82240:intron 40.0%
GCTCGGATTAAAATCTGATG+CGG + chr4.2:74956696-74956715 MS.gene82240:intron 40.0%
GTCTGCAAAGATTTCTTCAC+AGG + chr4.2:74958037-74958056 MS.gene82240:CDS 40.0%
TAGAGAAGTAGTAGCTCACT+TGG - chr4.2:74958771-74958790 None:intergenic 40.0%
TGCATATATCTGCTGTCTTC+AGG - chr4.2:74959184-74959203 None:intergenic 40.0%
TGGACATTAGCACAATGGTA+AGG - chr4.2:74956647-74956666 None:intergenic 40.0%
TGTACAGTGCTTAGAATTGG+TGG + chr4.2:74957935-74957954 MS.gene82240:intron 40.0%
TGTTAAATGGCGAAATGACG+AGG + chr4.2:74957694-74957713 MS.gene82240:intron 40.0%
TGTTGAACTGGATTGAATCG+GGG + chr4.2:74958878-74958897 MS.gene82240:intron 40.0%
TTCTTCCACTATTGTTAGCC+AGG + chr4.2:74959223-74959242 MS.gene82240:CDS 40.0%
TTTCAGCCCTGTATATAACC+TGG + chr4.2:74956965-74956984 MS.gene82240:intron 40.0%
! AACCAGCGAATTTACTGGTT+CGG - chr4.2:74958831-74958850 None:intergenic 40.0%
! CTTTTGCAAAGATACCGTTG+CGG + chr4.2:74958079-74958098 MS.gene82240:CDS 40.0%
! GGCTAACAATAGTGGAAGAA+GGG - chr4.2:74959223-74959242 None:intergenic 40.0%
! TATTATTGGCCTAAGCGCAA+TGG - chr4.2:74956343-74956362 None:intergenic 40.0%
! TGGCTAACAATAGTGGAAGA+AGG - chr4.2:74959224-74959243 None:intergenic 40.0%
!!! AAGAGGATGGTTTTCGAGTA+TGG + chr4.2:74959128-74959147 MS.gene82240:CDS 40.0%
!!! GTTTTTGGTTTGTAACCTCG+AGG - chr4.2:74956625-74956644 None:intergenic 40.0%
AAATGGCGAAATGACGAGGT+GGG + chr4.2:74957698-74957717 MS.gene82240:intron 45.0%
AACAGAGCTGCAGTATAACC+GGG - chr4.2:74958862-74958881 None:intergenic 45.0%
AAGGGTGGACATTAGCACAA+TGG - chr4.2:74956652-74956671 None:intergenic 45.0%
AGAAGTTGAGTTAGGGCTAG+TGG + chr4.2:74956389-74956408 MS.gene82240:intron 45.0%
AGCTTCCTGGCTAACAATAG+TGG - chr4.2:74959231-74959250 None:intergenic 45.0%
AGTAGCTCACTTGGCACTAA+GGG - chr4.2:74958762-74958781 None:intergenic 45.0%
ATACTGCAGCTCTGTTGAAC+TGG + chr4.2:74958866-74958885 MS.gene82240:intron 45.0%
ATGATCCCTTCATGTGAGGT+TGG + chr4.2:74956557-74956576 MS.gene82240:intron 45.0%
CAACAGAGCTGCAGTATAAC+CGG - chr4.2:74958863-74958882 None:intergenic 45.0%
CTCCCGAGTCATGGTTTAAT+TGG - chr4.2:74958958-74958977 None:intergenic 45.0%
TAAATGGCGAAATGACGAGG+TGG + chr4.2:74957697-74957716 MS.gene82240:intron 45.0%
TAGTAGCTCACTTGGCACTA+AGG - chr4.2:74958763-74958782 None:intergenic 45.0%
TCAACCAACCCTGGTTGAAA+AGG - chr4.2:74958909-74958928 None:intergenic 45.0%
TGAGATGATCCCTTCATGTG+AGG + chr4.2:74956553-74956572 MS.gene82240:intron 45.0%
TGATCCCTTCATGTGAGGTT+GGG + chr4.2:74956558-74956577 MS.gene82240:intron 45.0%
TTGATGTTGTCGATGTCGGA+TGG - chr4.2:74957823-74957842 None:intergenic 45.0%
TTGTGAAGGAATGCCCACAA+TGG + chr4.2:74957745-74957764 MS.gene82240:intron 45.0%
! AACAATAGTGGAAGAAGGGC+AGG - chr4.2:74959219-74959238 None:intergenic 45.0%
! AACTTTTCGCAGAGTTCTCC+AGG - chr4.2:74958005-74958024 None:intergenic 45.0%
! GAGGTGCCAGGTTTAAATCT+TGG - chr4.2:74956606-74956625 None:intergenic 45.0%
! GATGACGGCAAGTATTGTGA+AGG - chr4.2:74957459-74957478 None:intergenic 45.0%
! TGAACCTTTTCAACCAGGGT+TGG + chr4.2:74958902-74958921 MS.gene82240:intron 45.0%
!! GAAATGACGAGGTGGGTTTT+GGG + chr4.2:74957705-74957724 MS.gene82240:intron 45.0%
!! TTCGCTGGTTTGTATTTCCC+CGG + chr4.2:74958841-74958860 MS.gene82240:intron 45.0%
!!! GATTTTGTTGGGTGCTGCTT+GGG + chr4.2:74956025-74956044 MS.gene82240:CDS 45.0%
!!! GGGTTTTGGGTAACTGACAT+AGG + chr4.2:74957718-74957737 MS.gene82240:intron 45.0%
!!! TGATTTTGTTGGGTGCTGCT+TGG + chr4.2:74956024-74956043 MS.gene82240:CDS 45.0%
AATTCCGCTAGGTGAGGTCA+GGG + chr4.2:74956278-74956297 MS.gene82240:intron 50.0%
ACAGAGCTGCAGTATAACCG+GGG - chr4.2:74958861-74958880 None:intergenic 50.0%
CAAACCTGCTCGCTGAAAGA+CGG - chr4.2:74957484-74957503 None:intergenic 50.0%
CAATGCACGAGAATTCCGCT+AGG + chr4.2:74956267-74956286 MS.gene82240:intron 50.0%
CGCTGAAAGACGGAAGATGA+CGG - chr4.2:74957474-74957493 None:intergenic 50.0%
CTTCTGTGATTGCAGCTTCC+TGG - chr4.2:74959244-74959263 None:intergenic 50.0%
GACCGAACCAGTAAATTCGC+TGG + chr4.2:74958826-74958845 MS.gene82240:intron 50.0%
GATTTAAACCTGGCACCTCG+AGG + chr4.2:74956607-74956626 MS.gene82240:intron 50.0%
GCAAGAGAGTTTGCAAACCC+TGG + chr4.2:74956056-74956075 MS.gene82240:CDS 50.0%
GCTTCCCAACCTCACATGAA+GGG - chr4.2:74956565-74956584 None:intergenic 50.0%
GTAGCTCACTTGGCACTAAG+GGG - chr4.2:74958761-74958780 None:intergenic 50.0%
TCTTTGCAGACTCGCAAAGG+TGG - chr4.2:74958028-74958047 None:intergenic 50.0%
TGTCGATGTCGGATGGCATA+AGG - chr4.2:74957816-74957835 None:intergenic 50.0%
! GGGCTGAACCTTTTCAACCA+GGG + chr4.2:74958898-74958917 MS.gene82240:intron 50.0%
! TAAACCGGTTCAACCAACCC+TGG - chr4.2:74958918-74958937 None:intergenic 50.0%
!! CGAAATGACGAGGTGGGTTT+TGG + chr4.2:74957704-74957723 MS.gene82240:intron 50.0%
!! TCAACCAGGGTTGGTTGAAC+CGG + chr4.2:74958911-74958930 MS.gene82240:intron 50.0%
AACCTATGGCGCTGCCATTG+TGG - chr4.2:74957762-74957781 None:intergenic 55.0%
ACCTATGGCGCTGCCATTGT+GGG - chr4.2:74957761-74957780 None:intergenic 55.0%
CACGAGAATTCCGCTAGGTG+AGG + chr4.2:74956272-74956291 MS.gene82240:intron 55.0%
GAATTCCGCTAGGTGAGGTC+AGG + chr4.2:74956277-74956296 MS.gene82240:intron 55.0%
GGCTTCCCAACCTCACATGA+AGG - chr4.2:74956566-74956585 None:intergenic 55.0%
TCTTCCGTCTTTCAGCGAGC+AGG + chr4.2:74957477-74957496 MS.gene82240:CDS 55.0%
! CCATGACTCGGGAGTTTCAC+CGG + chr4.2:74958964-74958983 MS.gene82240:intron 55.0%
! GGGGCTGAACCTTTTCAACC+AGG + chr4.2:74958897-74958916 MS.gene82240:intron 55.0%
! GTTTGTAACCTCGAGGTGCC+AGG - chr4.2:74956618-74956637 None:intergenic 55.0%
CCGGTGAAACTCCCGAGTCA+TGG - chr4.2:74958967-74958986 None:intergenic 60.0%
GCCCACAATGGCAGCGCCAT+AGG + chr4.2:74957757-74957776 MS.gene82240:intron 65.0%
GGCGCCCTGACCTCACCTAG+CGG - chr4.2:74956285-74956304 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 74955996 74959289 74955996 ID=MS.gene82240
chr4.2 mRNA 74955996 74959289 74955996 ID=MS.gene82240.t1;Parent=MS.gene82240
chr4.2 exon 74955996 74956077 74955996 ID=MS.gene82240.t1.exon1;Parent=MS.gene82240.t1
chr4.2 CDS 74955996 74956077 74955996 ID=cds.MS.gene82240.t1;Parent=MS.gene82240.t1
chr4.2 exon 74957353 74957498 74957353 ID=MS.gene82240.t1.exon2;Parent=MS.gene82240.t1
chr4.2 CDS 74957353 74957498 74957353 ID=cds.MS.gene82240.t1;Parent=MS.gene82240.t1
chr4.2 exon 74957942 74958136 74957942 ID=MS.gene82240.t1.exon3;Parent=MS.gene82240.t1
chr4.2 CDS 74957942 74958136 74957942 ID=cds.MS.gene82240.t1;Parent=MS.gene82240.t1
chr4.2 exon 74958290 74958349 74958290 ID=MS.gene82240.t1.exon4;Parent=MS.gene82240.t1
chr4.2 CDS 74958290 74958349 74958290 ID=cds.MS.gene82240.t1;Parent=MS.gene82240.t1
chr4.2 exon 74959125 74959289 74959125 ID=MS.gene82240.t1.exon5;Parent=MS.gene82240.t1
chr4.2 CDS 74959125 74959289 74959125 ID=cds.MS.gene82240.t1;Parent=MS.gene82240.t1
Gene Sequence

>MS.gene82240

ATGGAAAGCAAAATAGGGCTTTTATTTCTGATTTTGTTGGGTGCTGCTTGGGCTTGTGATGCAAGAGAGTTTGCAAACCCTGGTAATTAACAATGTTGGCTAATACTCATGTTCTTTTTTCTTTTCTTTTTTGCCTAGATGGTTTGAAGTTTATTTATTTATGTAATCTTAGAAGATGGTGTAAAGTACAAAGATTAATAGGTTACTTTTAGCCATCAATGATAAACAAGAAAATAATTGTTGGCATTCTAAACTAGCATATAGGGCGGCTCAATGCACGAGAATTCCGCTAGGTGAGGTCAGGGCGCCAATCAGAGAGATTGTGTCTAGGATTGATCTTCTTACCATTGCGCTTAGGCCAATAATATATAGGGCGAGAAACGACTTAACATAAGAAGTTGAGTTAGGGCTAGTGGACCCTCTCATGTGTAATTTGCAAATGAGAGATTAAGTTAAGATCTCTCACCATTCAAACTCATTCTATCTAAATTTCACATGTGTTTCTCTTTATGAATTGTGAGAGATCGTACTTTACTCTTTGATGTGTAAATTACACATGAGATGATCCCTTCATGTGAGGTTGGGAAGCCAAACAAAGAGACTGTTTCCAAGATTTAAACCTGGCACCTCGAGGTTACAAACCAAAAACCTTACCATTGTGCTAATGTCCACCCTTAGAACCTAAATTAGTCCAAATTACGCTCGGATTAAAATCTGATGCGGAAAAGAAGTTTCTAAGTATTGAGATATGCAATTTTCATTAGTAAGGTACTACCAAATAAGTGAAGTAGCCAAAAGAATGTGATGGAGAAAAATAAGATAAACCTATTGAGAAAAGGCATTCTGAAAGGCCAAAAAATCCAAATATTTTTGAATTGAAACAGACCCTTTTCACCGATATGTTACCTGTTCTTATGTAGTCGACTTTTTTTAGTATAGCCGGTTTTCCTTCAAAAAAAAAGTGTAGCTTTTCAGCCCTGTATATAACCTGGATTCTGTTTCTGATTGTATGATATATCAGGAATTTATCGATCAAGCCTAGTTGATTGAGTTGCTTAGATTGATTTCCAGAGGTTCATGTATTCAATTGTTCCTTCTATGGTTGATATTTGATATGGTTCTGTTCATTTGATGGGAAACGAAGACCAATGCAAAATTTGTTAACTTCCCTTAGCCTATAATGTAGATGTATGAATTAATGAAATTTCAAATCTACGCCCATTTCTTAAGTACTTTCGCCTATAATATCATATGTACATTTTTACACTTAAAAAAAAAAAAATTGTACTAAACATAGTTAGAGTTTTGAAATACAAATGTAAAATCATTCATCTTTTGACATATCTTTCATATGCAGAATTAAACAAAAAACCAGATGCATGTTCAATTTGTGAGGAATATACTACTGAGATACTTGATTATCTAAAGGATAACAAGACTCAGGCAAAGATCATTGATGACCTTCACAATACTTGCCGTCATCTTCCGTCTTTCAGCGAGCAGGTTTGTCTCCTTCGCTTGATTCTATTTTGTTTACATGCGTTGTTGCAAATTTTGACTTGAGTTTTTTGCTTTTATGTTTAACATTTTATCTACACATCTCAGCTCGCTTTTTATCTATACCTATATATAAAAGGGATGCCAAGTTTTGGTGTAGCCATTTTTTCTTCCGATTTTACTCTTCATTTAAACCAGTGTTGTTAAATGGCGAAATGACGAGGTGGGTTTTGGGTAACTGACATAGGCAATTTGTGAAGGAATGCCCACAATGGCAGCGCCATAGGTTGCAATGACATGTTTATATGGCTGCTTTTTTGCCTTATGCCATCCGACATCGACAACATCAATTTAATGAATTTATAGTGTGTAGATTTGTCAAGGAATGAGAACTTATCAGGCCTTATTTTTAACTCTTCTCTGTTTGTTTTGTATTGCACATTGTACAGTGCTTAGAATTGGTGGATCATCATGTTCCGATTTTCTTCTCAAAAATAGCTCTAATGAAGCCTGGAGAACTCTGCGAAAAGTTCCACCTTTGCGAGTCTGCAAAGATTTCTTCACAGGTTCATGGAAATAGTTGTGGCTTTTGCAAAGATACCGTTGCGGAATTATTGGTTGAGTTGAATGATCCTGAAACTAAGGTTAGTGATTTTACTGCCGTACATTATCCTTCCTTAATATGAGGAGCATTAGAGATTCATATCGTGAATCAAAATAACTACTTTCTGAATTGGTATTCGAATCTTATTATGAATCGTAAGATACATTACTAATACTTTATTATAAAATGACAGCTAGAGATAATTCAGAAACTACTAAAGGCATGCAATAATATGGAGAAGTATAAGAAGGAGGTAAAGAAAATGAGAACTTCCTTTTGTTTTCTCTTTTTTTTATTGAATTACCCTTTTATTATTAAATGTCATTTTGCATGAGATCCTTTGGTTACATAAAATTAATTTTCATATTCTTATTCAATTGATTACAATGATTTTTCTGGAAGGTCAGATACCTTACCTAGTCAGTAATTGTTTTATTTTTTTAATGACATATGTTAGTATGTTAATTGTTATATAAGAGAAGACGAAGATAAATTGCTCATAATAAACTGTGTCAAAATTTGTTTCCGTGATCTATCAAGAATAGCTTACGAAAGTAAACTAGGATCGACTTTTGGACATATCATAAGTTAATTTTATACTCTTCCTAATATTTGCACAAGCGTTTGTATTATAAATTGGAAAATAAGTTATTTTGAACGTCCCCTTAGTGCCAAGTGAGCTACTACTTCTCTACTAGAAAAGCACAACAATACCAATGTTTTAAATCTGACCGAACCAGTAAATTCGCTGGTTTGTATTTCCCCGGTTATACTGCAGCTCTGTTGAACTGGATTGAATCGGGGCTGAACCTTTTCAACCAGGGTTGGTTGAACCGGTTTATTTGTTGCATATTTTAACCAATTAAACCATGACTCGGGAGTTTCACCGGTTTGATCTCTTATCCGTTTTTTATAACATTGACCAATTTTTGCAGTTACATATATGGAAGTCGCAAGAGTACATAATTTTCATAATACATTCATGTTTATCTATGATTTCGCATAATCGTCTAATTGTTTATTTACAGTGCAAGAGGATGGTTTTCGAGTATGGACCATTGATTCTAGTCAATGCAGAGAAGTACCTGAAGACAGCAGATATATGCACTACACTACATGCCTGCCCTTCTTCCACTATTGTTAGCCAGGAAGCTGCAATCACAGAAGAAACACCATTGTTTTCTGACTCTTAA
Protein sequence

>MS.gene82240.t1

MESKIGLLFLILLGAAWACDAREFANPELNKKPDACSICEEYTTEILDYLKDNKTQAKIIDDLHNTCRHLPSFSEQCLELVDHHVPIFFSKIALMKPGELCEKFHLCESAKISSQVHGNSCGFCKDTVAELLVELNDPETKLEIIQKLLKACNNMEKYKKECKRMVFEYGPLILVNAEKYLKTADICTTLHACPSSTIVSQEAAITEETPLFSDS