Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82355.t1 | RHN62028.1 | 83.3 | 84 | 14 | 0 | 4 | 87 | 73 | 156 | 1.00E-26 | 129 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82355.t1 | A0A396IB81 | 83.3 | 84 | 14 | 0 | 4 | 87 | 73 | 156 | 7.6e-27 | 129.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055946 | MS.gene82355 | 0.841205 | 5.47E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82355.t1 | MTR_4g082883 | 51.316 | 76 | 31 | 2 | 16 | 87 | 26 | 99 | 9.21e-12 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACTTGGTAGAAACTTATT+TGG | 0.193374 | 4.2:+26784275 | None:intergenic |
| CACACCTGCTTCTTCGGTTT+TGG | 0.199931 | 4.2:+26784094 | None:intergenic |
| GTTTCTACCAAGTGAGGTTT+TGG | 0.234806 | 4.2:-26784267 | MS.gene82355:CDS |
| ATCAAGCACACCTGCTTCTT+CGG | 0.347454 | 4.2:+26784088 | None:intergenic |
| TTTGGCAGGCTCCACAGGTT+TGG | 0.367487 | 4.2:+26784112 | None:intergenic |
| CAGTTGTAGAGCAAATTGAT+AGG | 0.431190 | 4.2:-26784154 | MS.gene82355:CDS |
| GAAGAAGCAGGTGTGCTTGA+TGG | 0.500199 | 4.2:-26784086 | MS.gene82355:CDS |
| GCACTCCCCAAAACCTCACT+TGG | 0.541506 | 4.2:+26784260 | None:intergenic |
| CCTGCCAAAACCGAAGAAGC+AGG | 0.546448 | 4.2:-26784098 | MS.gene82355:CDS |
| AAATAAGTTTCTACCAAGTG+AGG | 0.567992 | 4.2:-26784273 | MS.gene82355:CDS |
| AGTTGTAGAGCAAATTGATA+GGG | 0.586465 | 4.2:-26784153 | MS.gene82355:CDS |
| TTATGTTCATTCCAAACCTG+TGG | 0.590330 | 4.2:-26784123 | MS.gene82355:CDS |
| CAAACAACTGTAGTGAAACA+AGG | 0.639740 | 4.2:-26784215 | MS.gene82355:CDS |
| TGTAGTGAAACAAGGTGAGA+TGG | 0.645152 | 4.2:-26784207 | MS.gene82355:CDS |
| ATGGTTGCAACTTCTGCAGA+GGG | 0.653759 | 4.2:-26784188 | MS.gene82355:CDS |
| GATGGTTGCAACTTCTGCAG+AGG | 0.678769 | 4.2:-26784189 | MS.gene82355:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATAAGTTTCTACCAAGTG+AGG | - | chr4.2:26784072-26784091 | MS.gene82355:CDS | 30.0% | |
| AGTTGTAGAGCAAATTGATA+GGG | - | chr4.2:26784192-26784211 | MS.gene82355:CDS | 30.0% | |
| TCACTTGGTAGAAACTTATT+TGG | + | chr4.2:26784073-26784092 | None:intergenic | 30.0% | |
| CAAACAACTGTAGTGAAACA+AGG | - | chr4.2:26784130-26784149 | MS.gene82355:CDS | 35.0% | |
| CAGTTGTAGAGCAAATTGAT+AGG | - | chr4.2:26784191-26784210 | MS.gene82355:CDS | 35.0% | |
| TTATGTTCATTCCAAACCTG+TGG | - | chr4.2:26784222-26784241 | MS.gene82355:CDS | 35.0% | |
| ! | GATGGTGTTAAAAAGACTGT+TGG | - | chr4.2:26784277-26784296 | MS.gene82355:CDS | 35.0% |
| ! | TTTCTACCAAGTGAGGTTTT+GGG | - | chr4.2:26784079-26784098 | MS.gene82355:CDS | 35.0% |
| GGTGTTAAAAAGACTGTTGG+AGG | - | chr4.2:26784280-26784299 | MS.gene82355:CDS | 40.0% | |
| TGTAGTGAAACAAGGTGAGA+TGG | - | chr4.2:26784138-26784157 | MS.gene82355:CDS | 40.0% | |
| ! | GTTTCTACCAAGTGAGGTTT+TGG | - | chr4.2:26784078-26784097 | MS.gene82355:CDS | 40.0% |
| ! | TTCTACCAAGTGAGGTTTTG+GGG | - | chr4.2:26784080-26784099 | MS.gene82355:CDS | 40.0% |
| ATCAAGCACACCTGCTTCTT+CGG | + | chr4.2:26784260-26784279 | None:intergenic | 45.0% | |
| ATGGTTGCAACTTCTGCAGA+GGG | - | chr4.2:26784157-26784176 | MS.gene82355:CDS | 45.0% | |
| GAAGAAGCAGGTGTGCTTGA+TGG | - | chr4.2:26784259-26784278 | MS.gene82355:CDS | 50.0% | |
| GATGGTTGCAACTTCTGCAG+AGG | - | chr4.2:26784156-26784175 | MS.gene82355:CDS | 50.0% | |
| ! | CACACCTGCTTCTTCGGTTT+TGG | + | chr4.2:26784254-26784273 | None:intergenic | 50.0% |
| !!! | TGAGGTTTTGGGGAGTGCAA+AGG | - | chr4.2:26784090-26784109 | MS.gene82355:CDS | 50.0% |
| CCTGCCAAAACCGAAGAAGC+AGG | - | chr4.2:26784247-26784266 | MS.gene82355:CDS | 55.0% | |
| GCACTCCCCAAAACCTCACT+TGG | + | chr4.2:26784088-26784107 | None:intergenic | 55.0% | |
| TTTGGCAGGCTCCACAGGTT+TGG | + | chr4.2:26784236-26784255 | None:intergenic | 55.0% | |
| !! | CCTGCTTCTTCGGTTTTGGC+AGG | + | chr4.2:26784250-26784269 | None:intergenic | 55.0% |
| !!! | GGTTTTGGGGAGTGCAAAGG+TGG | - | chr4.2:26784093-26784112 | MS.gene82355:CDS | 55.0% |
| !! | TCGGTTTTGGCAGGCTCCAC+AGG | + | chr4.2:26784241-26784260 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 26784052 | 26784315 | 26784052 | ID=MS.gene82355 |
| chr4.2 | mRNA | 26784052 | 26784315 | 26784052 | ID=MS.gene82355.t1;Parent=MS.gene82355 |
| chr4.2 | exon | 26784052 | 26784315 | 26784052 | ID=MS.gene82355.t1.exon1;Parent=MS.gene82355.t1 |
| chr4.2 | CDS | 26784052 | 26784315 | 26784052 | ID=cds.MS.gene82355.t1;Parent=MS.gene82355.t1 |
| Gene Sequence |
| Protein sequence |
