Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82357.t1 | XP_003607784.1 | 93.8 | 81 | 5 | 0 | 1 | 81 | 1 | 81 | 1.50E-14 | 88.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82357.t1 | G7JHG4 | 93.8 | 81 | 5 | 0 | 1 | 81 | 1 | 81 | 1.1e-14 | 88.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04991 | MS.gene82357 | -0.80094 | 1.14E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82357.t1 | MTR_4g082900 | 91.358 | 81 | 7 | 0 | 1 | 81 | 1 | 81 | 1.14e-39 | 125 |
| MS.gene82357.t1 | MTR_4g082883 | 64.865 | 74 | 26 | 0 | 8 | 81 | 26 | 99 | 4.27e-25 | 89.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCTGGACCTAACGATATTT+TGG | 0.245207 | 4.2:-26775819 | MS.gene82357:CDS |
| AAAACCAAAGGTGCGGTTAA+TGG | 0.283737 | 4.2:-26775632 | MS.gene82357:CDS |
| TTTGCCAATAGGATCTGGTT+TGG | 0.364938 | 4.2:+26775652 | None:intergenic |
| TTGCCAATAGGATCTGGTTT+GGG | 0.376061 | 4.2:+26775653 | None:intergenic |
| AGCACCATTAACCGCACCTT+TGG | 0.426753 | 4.2:+26775628 | None:intergenic |
| GCACTTGCCAAAATATCGTT+AGG | 0.455143 | 4.2:+26775812 | None:intergenic |
| AAACCCAAACCAGATCCTAT+TGG | 0.473112 | 4.2:-26775656 | MS.gene82357:CDS |
| TAAACAACAAAATCGATGGC+TGG | 0.494913 | 4.2:-26775836 | None:intergenic |
| GGTGCTACAAAGTCTGTTGG+AGG | 0.564564 | 4.2:-26775611 | MS.gene82357:CDS |
| ACTGTAGGAAATGCTGCAGC+TGG | 0.565617 | 4.2:-26775761 | MS.gene82357:CDS |
| GCTGGTGTTCATAAGACTGT+AGG | 0.573068 | 4.2:-26775710 | MS.gene82357:CDS |
| AATGGTGCTACAAAGTCTGT+TGG | 0.575777 | 4.2:-26775614 | MS.gene82357:CDS |
| ACTGTGGGAAATGCTGCAGC+TGG | 0.580044 | 4.2:-26775728 | MS.gene82357:CDS |
| GCTTCAGCACAGAAAACTGT+AGG | 0.594928 | 4.2:-26775776 | MS.gene82357:CDS |
| GATCCTATTGGCAAAACCAA+AGG | 0.604323 | 4.2:-26775644 | MS.gene82357:CDS |
| AGCTGGTGCCCAGAAAACTG+TGG | 0.654418 | 4.2:-26775744 | MS.gene82357:CDS |
| TGTAGGAGACTATGCTACCA+TGG | 0.655972 | 4.2:-26775693 | MS.gene82357:CDS |
| TATTGGCAAAACCAAAGGTG+CGG | 0.681681 | 4.2:-26775639 | MS.gene82357:CDS |
| GCTGGTGCCCAGAAAACTGT+GGG | 0.683027 | 4.2:-26775743 | MS.gene82357:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTTACATAATCAGCAGCCA+TGG | + | chr4.2:26775746-26775765 | None:intergenic | 35.0% |
| AAACCCAAACCAGATCCTAT+TGG | - | chr4.2:26775763-26775782 | MS.gene82357:CDS | 40.0% | |
| GATCCTATTGGCAAAACCAA+AGG | - | chr4.2:26775775-26775794 | MS.gene82357:CDS | 40.0% | |
| GCACTTGCCAAAATATCGTT+AGG | + | chr4.2:26775610-26775629 | None:intergenic | 40.0% | |
| TATTGGCAAAACCAAAGGTG+CGG | - | chr4.2:26775780-26775799 | MS.gene82357:CDS | 40.0% | |
| TTTGCCAATAGGATCTGGTT+TGG | + | chr4.2:26775770-26775789 | None:intergenic | 40.0% | |
| ! | AAAACCAAAGGTGCGGTTAA+TGG | - | chr4.2:26775787-26775806 | MS.gene82357:CDS | 40.0% |
| ! | AGCATTTCCCACAGTTTTCT+GGG | + | chr4.2:26775686-26775705 | None:intergenic | 40.0% |
| ! | TTGCCAATAGGATCTGGTTT+GGG | + | chr4.2:26775769-26775788 | None:intergenic | 40.0% |
| !! | AATGGTGCTACAAAGTCTGT+TGG | - | chr4.2:26775805-26775824 | MS.gene82357:CDS | 40.0% |
| !!! | CGATATTTTGGCAAGTGCAA+AGG | - | chr4.2:26775612-26775631 | MS.gene82357:CDS | 40.0% |
| !!! | TTGGTTTTGCCAATAGGATC+TGG | + | chr4.2:26775775-26775794 | None:intergenic | 40.0% |
| GCTGGTGTTCATAAGACTGT+AGG | - | chr4.2:26775709-26775728 | MS.gene82357:CDS | 45.0% | |
| GCTTCAGCACAGAAAACTGT+AGG | - | chr4.2:26775643-26775662 | MS.gene82357:CDS | 45.0% | |
| TGTAGGAGACTATGCTACCA+TGG | - | chr4.2:26775726-26775745 | MS.gene82357:CDS | 45.0% | |
| ! | CAGCATTTCCCACAGTTTTC+TGG | + | chr4.2:26775687-26775706 | None:intergenic | 45.0% |
| ! | GGCTGGACCTAACGATATTT+TGG | - | chr4.2:26775600-26775619 | MS.gene82357:CDS | 45.0% |
| !!! | GCACCTTTGGTTTTGCCAAT+AGG | + | chr4.2:26775781-26775800 | None:intergenic | 45.0% |
| ACTGTAGGAAATGCTGCAGC+TGG | - | chr4.2:26775658-26775677 | MS.gene82357:CDS | 50.0% | |
| AGCACCATTAACCGCACCTT+TGG | + | chr4.2:26775794-26775813 | None:intergenic | 50.0% | |
| ! | GGTGCTACAAAGTCTGTTGG+AGG | - | chr4.2:26775808-26775827 | MS.gene82357:CDS | 50.0% |
| ACTGTGGGAAATGCTGCAGC+TGG | - | chr4.2:26775691-26775710 | MS.gene82357:CDS | 55.0% | |
| AGCTGGTGCCCAGAAAACTG+TGG | - | chr4.2:26775675-26775694 | MS.gene82357:CDS | 55.0% | |
| GCTGGTGCCCAGAAAACTGT+GGG | - | chr4.2:26775676-26775695 | MS.gene82357:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 26775598 | 26775843 | 26775598 | ID=MS.gene82357 |
| chr4.2 | mRNA | 26775598 | 26775843 | 26775598 | ID=MS.gene82357.t1;Parent=MS.gene82357 |
| chr4.2 | exon | 26775598 | 26775843 | 26775598 | ID=MS.gene82357.t1.exon1;Parent=MS.gene82357.t1 |
| chr4.2 | CDS | 26775598 | 26775843 | 26775598 | ID=cds.MS.gene82357.t1;Parent=MS.gene82357.t1 |
| Gene Sequence |
| Protein sequence |
