Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82609.t1 | KEH21125.1 | 65.8 | 190 | 59 | 2 | 1 | 186 | 99 | 286 | 1.20E-60 | 243 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82609.t1 | A0A072U5I8 | 65.8 | 190 | 59 | 2 | 1 | 186 | 99 | 286 | 8.6e-61 | 243.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054985 | MS.gene82609 | 0.897509 | 1.31E-76 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82609.t1 | MTR_8g098880 | 65.789 | 190 | 59 | 2 | 1 | 186 | 99 | 286 | 4.05e-82 | 246 |
| MS.gene82609.t1 | MTR_7g067580 | 66.492 | 191 | 57 | 3 | 1 | 186 | 21 | 209 | 1.47e-79 | 236 |
| MS.gene82609.t1 | MTR_7g066970 | 64.740 | 173 | 55 | 2 | 18 | 186 | 106 | 276 | 1.09e-59 | 188 |
| MS.gene82609.t1 | MTR_7g066880 | 46.111 | 180 | 72 | 2 | 19 | 198 | 89 | 243 | 1.59e-47 | 159 |
| MS.gene82609.t1 | MTR_7g066890 | 45.550 | 191 | 78 | 4 | 10 | 199 | 87 | 252 | 1.54e-42 | 147 |
| MS.gene82609.t1 | MTR_7g066890 | 45.550 | 191 | 78 | 4 | 10 | 199 | 189 | 354 | 1.79e-41 | 146 |
| MS.gene82609.t1 | MTR_7g066920 | 47.107 | 121 | 39 | 2 | 72 | 192 | 1 | 96 | 7.66e-32 | 111 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 50 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAATAGGAATTGGCCAAATT+TGG | 0.140871 | 3.1:-36147772 | None:intergenic |
| AATGGTTGGGAACCCAAATT+TGG | 0.239842 | 3.1:+36147759 | MS.gene82609:CDS |
| AAGTACCGAGACATCGTTAA+AGG | 0.256077 | 3.1:+36147487 | MS.gene82609:CDS |
| AATCCATCCCAGAGATTTAA+TGG | 0.256600 | 3.1:+36147445 | MS.gene82609:CDS |
| GAATTAGTTTCAGAGATAAT+AGG | 0.300684 | 3.1:-36147788 | None:intergenic |
| GGAAGATGATTACTCCTTGC+AGG | 0.323733 | 3.1:-36147632 | None:intergenic |
| TCGGTTCTACCTTCCCATTT+AGG | 0.341422 | 3.1:-36147698 | None:intergenic |
| CCTTGATACATAGGGTAACT+TGG | 0.376109 | 3.1:-36147413 | None:intergenic |
| AATAGGAATTGGCCAAATTT+GGG | 0.377581 | 3.1:-36147771 | None:intergenic |
| AGAATATTGTGCTATTTGTA+TGG | 0.390040 | 3.1:+36147346 | MS.gene82609:CDS |
| TTTCAAGATCCCTTGATTCA+TGG | 0.399012 | 3.1:-36147249 | None:intergenic |
| TTCACTTCCATTACGCTCAA+TGG | 0.406258 | 3.1:+36147580 | MS.gene82609:CDS |
| ACTACTAGCCATTAAATCTC+TGG | 0.407189 | 3.1:-36147453 | None:intergenic |
| AGAATCTTCAAAGTCACTTA+GGG | 0.410370 | 3.1:-36147381 | None:intergenic |
| GTTTCAGAGATAATAGGAAT+TGG | 0.414666 | 3.1:-36147782 | None:intergenic |
| TAGAATCTTCAAAGTCACTT+AGG | 0.416416 | 3.1:-36147382 | None:intergenic |
| TAATATGTTTATCTCTAGGT+TGG | 0.423404 | 3.1:-36147274 | None:intergenic |
| ATTAAAGTCATGCTCGTTTG+AGG | 0.428163 | 3.1:+36147832 | MS.gene82609:CDS |
| TGTGGAAGTATTGAAGTCAT+TGG | 0.439619 | 3.1:+36147529 | MS.gene82609:CDS |
| CTAGGTCCAATGGGAATAAC+CGG | 0.441881 | 3.1:-36147659 | None:intergenic |
| AAACTAATATGTTTATCTCT+AGG | 0.442036 | 3.1:-36147278 | None:intergenic |
| CCAATGGGAATAACCGGTCT+TGG | 0.447080 | 3.1:-36147653 | None:intergenic |
| TTCAAGATCCCTTGATTCAT+GGG | 0.450712 | 3.1:-36147248 | None:intergenic |
| CCAAGACCGGTTATTCCCAT+TGG | 0.456728 | 3.1:+36147653 | MS.gene82609:CDS |
| CCAAGTTACCCTATGTATCA+AGG | 0.466492 | 3.1:+36147413 | MS.gene82609:CDS |
| GATAAACATATTAGTTTACA+AGG | 0.469292 | 3.1:+36147284 | MS.gene82609:CDS |
| TTCAAGCTCCCTTGATACAT+AGG | 0.475861 | 3.1:-36147422 | None:intergenic |
| CATGATGACGACGACGATGA+TGG | 0.479371 | 3.1:+36147731 | MS.gene82609:CDS |
| CGATGATGGTTTGAAATGGT+TGG | 0.490172 | 3.1:+36147745 | MS.gene82609:CDS |
| TGCTTGAAACCTAGGTCCAA+TGG | 0.490476 | 3.1:-36147669 | None:intergenic |
| TCTTCCTCCATTGAGCGTAA+TGG | 0.493265 | 3.1:-36147587 | None:intergenic |
| GCTTGAAACCTAGGTCCAAT+GGG | 0.500023 | 3.1:-36147668 | None:intergenic |
| GAAACTAATTCAAAAGATGT+AGG | 0.500216 | 3.1:+36147800 | MS.gene82609:CDS |
| TCAGATTACCCCATGAATCA+AGG | 0.503710 | 3.1:+36147239 | None:intergenic |
| TCAAGCTCCCTTGATACATA+GGG | 0.506145 | 3.1:-36147421 | None:intergenic |
| GATGATGGTTTGAAATGGTT+GGG | 0.517681 | 3.1:+36147746 | MS.gene82609:CDS |
| ACGACGATGATGGTTTGAAA+TGG | 0.517695 | 3.1:+36147741 | MS.gene82609:CDS |
| CGGTTATTCCCATTGGACCT+AGG | 0.532003 | 3.1:+36147660 | MS.gene82609:CDS |
| TTTATCCTTTAACGATGTCT+CGG | 0.542742 | 3.1:-36147492 | None:intergenic |
| CTTGATACATAGGGTAACTT+GGG | 0.544147 | 3.1:-36147412 | None:intergenic |
| GAGTAATCATCTTCCAAGAC+CGG | 0.557162 | 3.1:+36147640 | MS.gene82609:CDS |
| CTACTAGCCATTAAATCTCT+GGG | 0.559045 | 3.1:-36147452 | None:intergenic |
| TAGCCATTAAATCTCTGGGA+TGG | 0.569005 | 3.1:-36147448 | None:intergenic |
| TCTTCTTTATCATCTTGAGA+AGG | 0.570123 | 3.1:-36147326 | None:intergenic |
| CAAGTTACCCTATGTATCAA+GGG | 0.593917 | 3.1:+36147414 | MS.gene82609:CDS |
| ACGCTCAATGGAGGAAGACA+TGG | 0.596812 | 3.1:+36147592 | MS.gene82609:CDS |
| CAGATTACCCCATGAATCAA+GGG | 0.603884 | 3.1:+36147240 | None:intergenic |
| TGGAAGTGAAGTCTTACCAT+CGG | 0.632413 | 3.1:-36147567 | None:intergenic |
| ACTTCCATTACGCTCAATGG+AGG | 0.645133 | 3.1:+36147583 | MS.gene82609:CDS |
| GAATCTTCAAAGTCACTTAG+GGG | 0.661294 | 3.1:-36147380 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACTAATATGTTTATCTCT+AGG | - | chr3.1:36147281-36147300 | None:intergenic | 20.0% |
| !! | TAAACCAAAAATAGAATTTG+TGG | + | chr3.1:36147511-36147530 | MS.gene82609:CDS | 20.0% |
| !!! | GATAAACATATTAGTTTACA+AGG | + | chr3.1:36147284-36147303 | MS.gene82609:CDS | 20.0% |
| ! | GAAACTAATTCAAAAGATGT+AGG | + | chr3.1:36147800-36147819 | MS.gene82609:CDS | 25.0% |
| ! | TAATATGTTTATCTCTAGGT+TGG | - | chr3.1:36147277-36147296 | None:intergenic | 25.0% |
| ! | TCAAGCAAAAATACCTAAAT+GGG | + | chr3.1:36147685-36147704 | MS.gene82609:CDS | 25.0% |
| ! | TTCAAGCAAAAATACCTAAA+TGG | + | chr3.1:36147684-36147703 | MS.gene82609:CDS | 25.0% |
| !! | ACTTCCACAAATTCTATTTT+TGG | - | chr3.1:36147518-36147537 | None:intergenic | 25.0% |
| !! | GAATTAGTTTCAGAGATAAT+AGG | - | chr3.1:36147791-36147810 | None:intergenic | 25.0% |
| !!! | AGAATATTGTGCTATTTGTA+TGG | + | chr3.1:36147346-36147365 | MS.gene82609:CDS | 25.0% |
| AATAGGAATTGGCCAAATTT+GGG | - | chr3.1:36147774-36147793 | None:intergenic | 30.0% | |
| AGAATCTTCAAAGTCACTTA+GGG | - | chr3.1:36147384-36147403 | None:intergenic | 30.0% | |
| GTTTCAGAGATAATAGGAAT+TGG | - | chr3.1:36147785-36147804 | None:intergenic | 30.0% | |
| TAATAGGAATTGGCCAAATT+TGG | - | chr3.1:36147775-36147794 | None:intergenic | 30.0% | |
| TAGAATCTTCAAAGTCACTT+AGG | - | chr3.1:36147385-36147404 | None:intergenic | 30.0% | |
| TCTTCTTTATCATCTTGAGA+AGG | - | chr3.1:36147329-36147348 | None:intergenic | 30.0% | |
| TTTATCCTTTAACGATGTCT+CGG | - | chr3.1:36147495-36147514 | None:intergenic | 30.0% | |
| ! | TCAACAAATTTTTACCTGCA+AGG | + | chr3.1:36147618-36147637 | MS.gene82609:CDS | 30.0% |
| !!! | GTCATCATGTTGTTTTATGT+CGG | - | chr3.1:36147720-36147739 | None:intergenic | 30.0% |
| AATCCATCCCAGAGATTTAA+TGG | + | chr3.1:36147445-36147464 | MS.gene82609:CDS | 35.0% | |
| ACTACTAGCCATTAAATCTC+TGG | - | chr3.1:36147456-36147475 | None:intergenic | 35.0% | |
| ATTAAAGTCATGCTCGTTTG+AGG | + | chr3.1:36147832-36147851 | MS.gene82609:CDS | 35.0% | |
| CAAGTTACCCTATGTATCAA+GGG | + | chr3.1:36147414-36147433 | MS.gene82609:CDS | 35.0% | |
| CTACTAGCCATTAAATCTCT+GGG | - | chr3.1:36147455-36147474 | None:intergenic | 35.0% | |
| CTTGATACATAGGGTAACTT+GGG | - | chr3.1:36147415-36147434 | None:intergenic | 35.0% | |
| GAATCTTCAAAGTCACTTAG+GGG | - | chr3.1:36147383-36147402 | None:intergenic | 35.0% | |
| GCAAAAATACCTAAATGGGA+AGG | + | chr3.1:36147689-36147708 | MS.gene82609:CDS | 35.0% | |
| ! | GATGATGGTTTGAAATGGTT+GGG | + | chr3.1:36147746-36147765 | MS.gene82609:CDS | 35.0% |
| !! | GGTATTTTTGCTTGAAACCT+AGG | - | chr3.1:36147680-36147699 | None:intergenic | 35.0% |
| !! | TGTGGAAGTATTGAAGTCAT+TGG | + | chr3.1:36147529-36147548 | MS.gene82609:CDS | 35.0% |
| AATGGTTGGGAACCCAAATT+TGG | + | chr3.1:36147759-36147778 | MS.gene82609:CDS | 40.0% | |
| CCAAGTTACCCTATGTATCA+AGG | + | chr3.1:36147413-36147432 | MS.gene82609:CDS | 40.0% | |
| CCTTGATACATAGGGTAACT+TGG | - | chr3.1:36147416-36147435 | None:intergenic | 40.0% | |
| GAGTAATCATCTTCCAAGAC+CGG | + | chr3.1:36147640-36147659 | MS.gene82609:CDS | 40.0% | |
| TCAAGCTCCCTTGATACATA+GGG | - | chr3.1:36147424-36147443 | None:intergenic | 40.0% | |
| TGGAAGTGAAGTCTTACCAT+CGG | - | chr3.1:36147570-36147589 | None:intergenic | 40.0% | |
| TTCAAGCTCCCTTGATACAT+AGG | - | chr3.1:36147425-36147444 | None:intergenic | 40.0% | |
| TTCACTTCCATTACGCTCAA+TGG | + | chr3.1:36147580-36147599 | MS.gene82609:CDS | 40.0% | |
| ! | ACGACGATGATGGTTTGAAA+TGG | + | chr3.1:36147741-36147760 | MS.gene82609:CDS | 40.0% |
| ! | CGATGATGGTTTGAAATGGT+TGG | + | chr3.1:36147745-36147764 | MS.gene82609:CDS | 40.0% |
| ! | GTCGATGAAGATTTTACCGA+TGG | + | chr3.1:36147551-36147570 | MS.gene82609:CDS | 40.0% |
| ! | TAGCCATTAAATCTCTGGGA+TGG | - | chr3.1:36147451-36147470 | None:intergenic | 40.0% |
| !! | AAGTACCGAGACATCGTTAA+AGG | + | chr3.1:36147487-36147506 | MS.gene82609:CDS | 40.0% |
| ACTTCCATTACGCTCAATGG+AGG | + | chr3.1:36147583-36147602 | MS.gene82609:CDS | 45.0% | |
| CTAGGTCCAATGGGAATAAC+CGG | - | chr3.1:36147662-36147681 | None:intergenic | 45.0% | |
| GCTTGAAACCTAGGTCCAAT+GGG | - | chr3.1:36147671-36147690 | None:intergenic | 45.0% | |
| GGAAGATGATTACTCCTTGC+AGG | - | chr3.1:36147635-36147654 | None:intergenic | 45.0% | |
| TCGGTTCTACCTTCCCATTT+AGG | - | chr3.1:36147701-36147720 | None:intergenic | 45.0% | |
| TCTTCCTCCATTGAGCGTAA+TGG | - | chr3.1:36147590-36147609 | None:intergenic | 45.0% | |
| TGCTTGAAACCTAGGTCCAA+TGG | - | chr3.1:36147672-36147691 | None:intergenic | 45.0% | |
| ACGCTCAATGGAGGAAGACA+TGG | + | chr3.1:36147592-36147611 | MS.gene82609:CDS | 50.0% | |
| CCAAGACCGGTTATTCCCAT+TGG | + | chr3.1:36147653-36147672 | MS.gene82609:CDS | 50.0% | |
| CCAATGGGAATAACCGGTCT+TGG | - | chr3.1:36147656-36147675 | None:intergenic | 50.0% | |
| CGGTTATTCCCATTGGACCT+AGG | + | chr3.1:36147660-36147679 | MS.gene82609:CDS | 50.0% | |
| ! | CATGATGACGACGACGATGA+TGG | + | chr3.1:36147731-36147750 | MS.gene82609:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 36147251 | 36147877 | 36147251 | ID=MS.gene82609 |
| chr3.1 | mRNA | 36147251 | 36147877 | 36147251 | ID=MS.gene82609.t1;Parent=MS.gene82609 |
| chr3.1 | exon | 36147251 | 36147877 | 36147251 | ID=MS.gene82609.t1.exon1;Parent=MS.gene82609.t1 |
| chr3.1 | CDS | 36147251 | 36147877 | 36147251 | ID=cds.MS.gene82609.t1;Parent=MS.gene82609.t1 |
| Gene Sequence |
| Protein sequence |
