Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054985.t1 | KEH21125.1 | 63.9 | 191 | 62 | 2 | 1 | 187 | 99 | 286 | 4.30E-58 | 234.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054985.t1 | A0A072U5I8 | 63.9 | 191 | 62 | 2 | 1 | 187 | 99 | 286 | 3.1e-58 | 234.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054985 | MS.gene45677 | 0.807467 | 4.98E-50 | -1.69E-46 |
| MS.gene054985 | MS.gene82609 | 0.897509 | 1.31E-76 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054985.t1 | MTR_8g098880 | 65.445 | 191 | 59 | 2 | 1 | 187 | 99 | 286 | 5.52e-82 | 246 |
| MS.gene054985.t1 | MTR_7g067580 | 66.146 | 192 | 57 | 3 | 1 | 187 | 21 | 209 | 2.60e-79 | 236 |
| MS.gene054985.t1 | MTR_7g066970 | 64.368 | 174 | 55 | 2 | 18 | 187 | 106 | 276 | 1.65e-59 | 188 |
| MS.gene054985.t1 | MTR_7g066880 | 45.856 | 181 | 72 | 2 | 19 | 199 | 89 | 243 | 3.26e-47 | 159 |
| MS.gene054985.t1 | MTR_7g066890 | 45.312 | 192 | 78 | 4 | 10 | 200 | 87 | 252 | 3.37e-42 | 146 |
| MS.gene054985.t1 | MTR_7g066890 | 45.312 | 192 | 78 | 4 | 10 | 200 | 189 | 354 | 4.04e-41 | 145 |
| MS.gene054985.t1 | MTR_7g066920 | 46.721 | 122 | 39 | 2 | 72 | 193 | 1 | 96 | 1.78e-31 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 50 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAATAGGAATTGGCCAAATT+TGG | 0.140871 | 7.4:-36613299 | None:intergenic |
| AATGGTTGGGAACCCAAATT+TGG | 0.239842 | 7.4:+36613286 | MS.gene054985:CDS |
| AAGTACCGAGACATCGTTAA+AGG | 0.256077 | 7.4:+36613011 | MS.gene054985:CDS |
| AATCCATCCCAGAGATTTAA+TGG | 0.256600 | 7.4:+36612969 | MS.gene054985:CDS |
| GTATTAGTTTCAGAGATAAT+AGG | 0.311467 | 7.4:-36613315 | None:intergenic |
| GGAAGATGATTACTCCTTGC+AGG | 0.323733 | 7.4:-36613156 | None:intergenic |
| TCGGTTCTACCTTCCCATTT+AGG | 0.341422 | 7.4:-36613222 | None:intergenic |
| CCTTGATACATAGGGTAACT+TGG | 0.376109 | 7.4:-36612937 | None:intergenic |
| AATAGGAATTGGCCAAATTT+GGG | 0.377581 | 7.4:-36613298 | None:intergenic |
| AGAATATTGTGCTATTTGTA+TGG | 0.390040 | 7.4:+36612870 | MS.gene054985:CDS |
| TTTCAAGATCCCTTGATTCA+TGG | 0.399012 | 7.4:-36612773 | None:intergenic |
| TTCACTTCCATTACGCTCAA+TGG | 0.406258 | 7.4:+36613104 | MS.gene054985:CDS |
| ACTACTAGCCATTAAATCTC+TGG | 0.407189 | 7.4:-36612977 | None:intergenic |
| AGAATCTTCAAAGTCACTTA+GGG | 0.410370 | 7.4:-36612905 | None:intergenic |
| GTTTCAGAGATAATAGGAAT+TGG | 0.414666 | 7.4:-36613309 | None:intergenic |
| TAGAATCTTCAAAGTCACTT+AGG | 0.416416 | 7.4:-36612906 | None:intergenic |
| TAATATGTTTATCTCTAGGT+TGG | 0.423404 | 7.4:-36612798 | None:intergenic |
| ATTAAAGTCATGCTCGTTTG+AGG | 0.428163 | 7.4:+36613359 | MS.gene054985:CDS |
| TGTGGAAGTATTGAAGTCAT+TGG | 0.439619 | 7.4:+36613053 | MS.gene054985:CDS |
| CTAGGTCCAATGGGAATAAC+CGG | 0.441881 | 7.4:-36613183 | None:intergenic |
| AAACTAATATGTTTATCTCT+AGG | 0.442036 | 7.4:-36612802 | None:intergenic |
| CCAATGGGAATAACCGGTCT+TGG | 0.447080 | 7.4:-36613177 | None:intergenic |
| TTCAAGATCCCTTGATTCAT+GGG | 0.450712 | 7.4:-36612772 | None:intergenic |
| CCAAGACCGGTTATTCCCAT+TGG | 0.456728 | 7.4:+36613177 | MS.gene054985:CDS |
| CCAAGTTACCCTATGTATCA+AGG | 0.466492 | 7.4:+36612937 | MS.gene054985:CDS |
| GATAAACATATTAGTTTACA+AGG | 0.469292 | 7.4:+36612808 | MS.gene054985:CDS |
| TTCAAGCTCCCTTGATACAT+AGG | 0.475861 | 7.4:-36612946 | None:intergenic |
| CGATGATGGTTTGAAATGGT+TGG | 0.490172 | 7.4:+36613272 | MS.gene054985:CDS |
| TGCTTGAAACCTAGGTCCAA+TGG | 0.490476 | 7.4:-36613193 | None:intergenic |
| TCTTCCTCCATTGAGCGTAA+TGG | 0.493265 | 7.4:-36613111 | None:intergenic |
| GCTTGAAACCTAGGTCCAAT+GGG | 0.500023 | 7.4:-36613192 | None:intergenic |
| TCAGATTACCCCATGAATCA+AGG | 0.503710 | 7.4:+36612763 | None:intergenic |
| TCAAGCTCCCTTGATACATA+GGG | 0.506145 | 7.4:-36612945 | None:intergenic |
| GATGATGGTTTGAAATGGTT+GGG | 0.517681 | 7.4:+36613273 | MS.gene054985:CDS |
| ACGACGATGATGGTTTGAAA+TGG | 0.517695 | 7.4:+36613268 | MS.gene054985:CDS |
| GATGACGACGACGACGATGA+TGG | 0.527360 | 7.4:+36613258 | MS.gene054985:CDS |
| CGGTTATTCCCATTGGACCT+AGG | 0.532003 | 7.4:+36613184 | MS.gene054985:CDS |
| GAAACTAATACAAAAGATGT+AGG | 0.532974 | 7.4:+36613327 | MS.gene054985:CDS |
| TTTATCCTTTAACGATGTCT+CGG | 0.542742 | 7.4:-36613016 | None:intergenic |
| CTTGATACATAGGGTAACTT+GGG | 0.544147 | 7.4:-36612936 | None:intergenic |
| GAGTAATCATCTTCCAAGAC+CGG | 0.557162 | 7.4:+36613164 | MS.gene054985:CDS |
| CTACTAGCCATTAAATCTCT+GGG | 0.559045 | 7.4:-36612976 | None:intergenic |
| TAGCCATTAAATCTCTGGGA+TGG | 0.569005 | 7.4:-36612972 | None:intergenic |
| TCTTCTTTATCATCTTGAGA+AGG | 0.570123 | 7.4:-36612850 | None:intergenic |
| CAAGTTACCCTATGTATCAA+GGG | 0.593917 | 7.4:+36612938 | MS.gene054985:CDS |
| ACGCTCAATGGAGGAAGACA+TGG | 0.596812 | 7.4:+36613116 | MS.gene054985:CDS |
| CAGATTACCCCATGAATCAA+GGG | 0.603884 | 7.4:+36612764 | None:intergenic |
| TGGAAGTGAAGTCTTACCAT+CGG | 0.632413 | 7.4:-36613091 | None:intergenic |
| ACTTCCATTACGCTCAATGG+AGG | 0.645133 | 7.4:+36613107 | MS.gene054985:CDS |
| GAATCTTCAAAGTCACTTAG+GGG | 0.661294 | 7.4:-36612904 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACTAATATGTTTATCTCT+AGG | - | chr7.4:36612805-36612824 | None:intergenic | 20.0% |
| !! | TAAACCAAAAATAGAATTTG+TGG | + | chr7.4:36613035-36613054 | MS.gene054985:CDS | 20.0% |
| !!! | GATAAACATATTAGTTTACA+AGG | + | chr7.4:36612808-36612827 | MS.gene054985:CDS | 20.0% |
| ! | GAAACTAATACAAAAGATGT+AGG | + | chr7.4:36613327-36613346 | MS.gene054985:CDS | 25.0% |
| ! | TAATATGTTTATCTCTAGGT+TGG | - | chr7.4:36612801-36612820 | None:intergenic | 25.0% |
| ! | TCAAGCAAAAATACCTAAAT+GGG | + | chr7.4:36613209-36613228 | MS.gene054985:CDS | 25.0% |
| ! | TTCAAGCAAAAATACCTAAA+TGG | + | chr7.4:36613208-36613227 | MS.gene054985:CDS | 25.0% |
| !! | ACTTCCACAAATTCTATTTT+TGG | - | chr7.4:36613042-36613061 | None:intergenic | 25.0% |
| !! | GTATTAGTTTCAGAGATAAT+AGG | - | chr7.4:36613318-36613337 | None:intergenic | 25.0% |
| !!! | AGAATATTGTGCTATTTGTA+TGG | + | chr7.4:36612870-36612889 | MS.gene054985:CDS | 25.0% |
| AATAGGAATTGGCCAAATTT+GGG | - | chr7.4:36613301-36613320 | None:intergenic | 30.0% | |
| AGAATCTTCAAAGTCACTTA+GGG | - | chr7.4:36612908-36612927 | None:intergenic | 30.0% | |
| GTTTCAGAGATAATAGGAAT+TGG | - | chr7.4:36613312-36613331 | None:intergenic | 30.0% | |
| TAATAGGAATTGGCCAAATT+TGG | - | chr7.4:36613302-36613321 | None:intergenic | 30.0% | |
| TAGAATCTTCAAAGTCACTT+AGG | - | chr7.4:36612909-36612928 | None:intergenic | 30.0% | |
| TCTTCTTTATCATCTTGAGA+AGG | - | chr7.4:36612853-36612872 | None:intergenic | 30.0% | |
| TTTATCCTTTAACGATGTCT+CGG | - | chr7.4:36613019-36613038 | None:intergenic | 30.0% | |
| ! | TCAACAAATTTTTACCTGCA+AGG | + | chr7.4:36613142-36613161 | MS.gene054985:CDS | 30.0% |
| !!! | GTCATCATGTTGTTTTATGT+CGG | - | chr7.4:36613244-36613263 | None:intergenic | 30.0% |
| AATCCATCCCAGAGATTTAA+TGG | + | chr7.4:36612969-36612988 | MS.gene054985:CDS | 35.0% | |
| ACTACTAGCCATTAAATCTC+TGG | - | chr7.4:36612980-36612999 | None:intergenic | 35.0% | |
| ATTAAAGTCATGCTCGTTTG+AGG | + | chr7.4:36613359-36613378 | MS.gene054985:CDS | 35.0% | |
| CAAGTTACCCTATGTATCAA+GGG | + | chr7.4:36612938-36612957 | MS.gene054985:CDS | 35.0% | |
| CTACTAGCCATTAAATCTCT+GGG | - | chr7.4:36612979-36612998 | None:intergenic | 35.0% | |
| CTTGATACATAGGGTAACTT+GGG | - | chr7.4:36612939-36612958 | None:intergenic | 35.0% | |
| GAATCTTCAAAGTCACTTAG+GGG | - | chr7.4:36612907-36612926 | None:intergenic | 35.0% | |
| GCAAAAATACCTAAATGGGA+AGG | + | chr7.4:36613213-36613232 | MS.gene054985:CDS | 35.0% | |
| ! | GATGATGGTTTGAAATGGTT+GGG | + | chr7.4:36613273-36613292 | MS.gene054985:CDS | 35.0% |
| !! | GGTATTTTTGCTTGAAACCT+AGG | - | chr7.4:36613204-36613223 | None:intergenic | 35.0% |
| !! | TGTGGAAGTATTGAAGTCAT+TGG | + | chr7.4:36613053-36613072 | MS.gene054985:CDS | 35.0% |
| AATGGTTGGGAACCCAAATT+TGG | + | chr7.4:36613286-36613305 | MS.gene054985:CDS | 40.0% | |
| CCAAGTTACCCTATGTATCA+AGG | + | chr7.4:36612937-36612956 | MS.gene054985:CDS | 40.0% | |
| CCTTGATACATAGGGTAACT+TGG | - | chr7.4:36612940-36612959 | None:intergenic | 40.0% | |
| GAGTAATCATCTTCCAAGAC+CGG | + | chr7.4:36613164-36613183 | MS.gene054985:CDS | 40.0% | |
| TCAAGCTCCCTTGATACATA+GGG | - | chr7.4:36612948-36612967 | None:intergenic | 40.0% | |
| TGGAAGTGAAGTCTTACCAT+CGG | - | chr7.4:36613094-36613113 | None:intergenic | 40.0% | |
| TTCAAGCTCCCTTGATACAT+AGG | - | chr7.4:36612949-36612968 | None:intergenic | 40.0% | |
| TTCACTTCCATTACGCTCAA+TGG | + | chr7.4:36613104-36613123 | MS.gene054985:CDS | 40.0% | |
| ! | ACGACGATGATGGTTTGAAA+TGG | + | chr7.4:36613268-36613287 | MS.gene054985:CDS | 40.0% |
| ! | CGATGATGGTTTGAAATGGT+TGG | + | chr7.4:36613272-36613291 | MS.gene054985:CDS | 40.0% |
| ! | GTCGATGAAGATTTTACCGA+TGG | + | chr7.4:36613075-36613094 | MS.gene054985:CDS | 40.0% |
| ! | TAGCCATTAAATCTCTGGGA+TGG | - | chr7.4:36612975-36612994 | None:intergenic | 40.0% |
| !! | AAGTACCGAGACATCGTTAA+AGG | + | chr7.4:36613011-36613030 | MS.gene054985:CDS | 40.0% |
| ACTTCCATTACGCTCAATGG+AGG | + | chr7.4:36613107-36613126 | MS.gene054985:CDS | 45.0% | |
| CTAGGTCCAATGGGAATAAC+CGG | - | chr7.4:36613186-36613205 | None:intergenic | 45.0% | |
| GCTTGAAACCTAGGTCCAAT+GGG | - | chr7.4:36613195-36613214 | None:intergenic | 45.0% | |
| GGAAGATGATTACTCCTTGC+AGG | - | chr7.4:36613159-36613178 | None:intergenic | 45.0% | |
| TCGGTTCTACCTTCCCATTT+AGG | - | chr7.4:36613225-36613244 | None:intergenic | 45.0% | |
| TCTTCCTCCATTGAGCGTAA+TGG | - | chr7.4:36613114-36613133 | None:intergenic | 45.0% | |
| TGCTTGAAACCTAGGTCCAA+TGG | - | chr7.4:36613196-36613215 | None:intergenic | 45.0% | |
| ACGCTCAATGGAGGAAGACA+TGG | + | chr7.4:36613116-36613135 | MS.gene054985:CDS | 50.0% | |
| CCAAGACCGGTTATTCCCAT+TGG | + | chr7.4:36613177-36613196 | MS.gene054985:CDS | 50.0% | |
| CCAATGGGAATAACCGGTCT+TGG | - | chr7.4:36613180-36613199 | None:intergenic | 50.0% | |
| CGGTTATTCCCATTGGACCT+AGG | + | chr7.4:36613184-36613203 | MS.gene054985:CDS | 50.0% | |
| GATGACGACGACGACGATGA+TGG | + | chr7.4:36613258-36613277 | MS.gene054985:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 36612775 | 36613404 | 36612775 | ID=MS.gene054985 |
| chr7.4 | mRNA | 36612775 | 36613404 | 36612775 | ID=MS.gene054985.t1;Parent=MS.gene054985 |
| chr7.4 | exon | 36612775 | 36613404 | 36612775 | ID=MS.gene054985.t1.exon1;Parent=MS.gene054985.t1 |
| chr7.4 | CDS | 36612775 | 36613404 | 36612775 | ID=cds.MS.gene054985.t1;Parent=MS.gene054985.t1 |
| Gene Sequence |
| Protein sequence |
