AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82783

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82783.t1 MTR_7g007440 92.857 98 7 0 1 98 1 98 1.41e-55 167
MS.gene82783.t1 MTR_7g082640 53.933 89 41 0 10 98 6 94 8.56e-35 114
MS.gene82783.t1 MTR_6g082480 49.383 81 41 0 18 98 12 92 7.29e-29 99.8
MS.gene82783.t1 MTR_1g109650 42.222 90 52 0 9 98 3 92 1.87e-26 93.6
MS.gene82783.t1 MTR_7g082590 42.045 88 51 0 10 97 6 93 3.68e-22 82.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82783.t1 AT3G18280 49.438 89 42 1 10 98 35 120 3.80e-30 103
MS.gene82783.t1 AT3G18280 49.438 89 45 0 10 98 8 96 6.05e-30 102
MS.gene82783.t1 AT1G48750 55.556 72 32 0 27 98 23 94 1.29e-28 99.4
MS.gene82783.t1 AT1G73780 33.673 98 65 0 1 98 1 98 5.04e-22 82.8
MS.gene82783.t1 AT1G66850 50.725 69 34 0 30 98 34 102 2.03e-21 81.3
MS.gene82783.t1 AT1G43666 42.391 92 49 2 9 98 6 95 5.27e-20 77.4
MS.gene82783.t1 AT1G43667 36.000 100 60 2 1 98 1 98 4.00e-19 75.5
MS.gene82783.t1 AT5G38170 45.588 68 37 0 31 98 36 103 4.76e-19 75.5
MS.gene82783.t1 AT5G38195 47.761 67 35 0 32 98 29 95 7.76e-19 74.7
MS.gene82783.t1 AT5G38160 41.176 68 40 0 31 98 36 103 1.29e-17 71.6
MS.gene82783.t1 AT5G38180 34.694 98 61 1 1 98 1 95 5.80e-17 69.7
MS.gene82783.t1 AT2G14846 45.161 62 34 0 37 98 38 99 1.31e-14 63.9
MS.gene82783.t1 AT1G43665 38.235 68 40 1 29 96 30 95 2.28e-14 63.9
MS.gene82783.t1 AT5G38197 35.714 98 61 2 1 98 1 96 9.05e-14 61.6
MS.gene82783.t1 AT1G07747 40.909 66 37 1 33 98 29 92 2.30e-13 60.5
MS.gene82783.t1 AT4G12825 33.333 90 54 2 9 98 12 95 3.47e-13 60.1
MS.gene82783.t1 AT3G57310 36.364 66 42 0 33 98 38 103 9.86e-13 59.3

Find 29 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAGAGTTCAGTCCTTGTTT+AGG 0.334416 6.1:+255254 MS.gene82783:CDS
GTCTCTGCTCTCTTAACTTC+TGG 0.337118 6.1:-255316 None:intergenic
TTTACTCTCCTGGTGCTAGA+AGG 0.339135 6.1:+255384 MS.gene82783:CDS
GAGCAGAGACCATGCCTTTG+TGG 0.411218 6.1:+255329 MS.gene82783:CDS
GAAGGGTTACTAGCTCTTGT+GGG 0.413579 6.1:+255402 MS.gene82783:CDS
TTCTGGCAACAAGCACTTGT+TGG 0.433827 6.1:-255299 None:intergenic
ATGATTGATGGCTAACAAGT+TGG 0.442427 6.1:-255434 None:intergenic
TAAATATACTGCTTGAGGCT+TGG 0.456606 6.1:-255365 None:intergenic
AAACAAGGACTGAACTCTAC+AGG 0.466330 6.1:-255251 None:intergenic
TGGCAACAAGCACTTGTTGG+TGG 0.497936 6.1:-255296 None:intergenic
AGAAGGGTTACTAGCTCTTG+TGG 0.498825 6.1:+255401 MS.gene82783:CDS
TGATTGATGGCTAACAAGTT+GGG 0.507677 6.1:-255433 None:intergenic
TGTTTATGCAGTAGTGGTTG+TGG 0.513787 6.1:+255175 MS.gene82783:CDS
AAGCAGTATATTTACTCTCC+TGG 0.517554 6.1:+255374 MS.gene82783:CDS
TGGTATTGTTTATGCAGTAG+TGG 0.524181 6.1:+255169 MS.gene82783:CDS
TTTGGGGAAGGAGACTTACA+TGG 0.529156 6.1:+255208 MS.gene82783:CDS
CTAGTAACCCTTCTAGCACC+AGG 0.531212 6.1:-255392 None:intergenic
TTACTCTCCTGGTGCTAGAA+GGG 0.532631 6.1:+255385 MS.gene82783:CDS
GCTTGAGGCTTGGATTCTTG+AGG 0.548134 6.1:-255355 None:intergenic
ATGAAGAAACACTCTAGCTA+TGG 0.561126 6.1:+255149 MS.gene82783:CDS
TGATGGCTAACAAGTTGGGA+AGG 0.576643 6.1:-255429 None:intergenic
GAGAGTAAATATACTGCTTG+AGG 0.582416 6.1:-255370 None:intergenic
AAGGGTTACTAGCTCTTGTG+GGG 0.597115 6.1:+255403 MS.gene82783:CDS
GAAGGAGACTTACATGGCAA+AGG 0.601332 6.1:+255214 MS.gene82783:CDS
AACAAGGACTGAACTCTACA+GGG 0.629235 6.1:-255250 None:intergenic
GATGGCTAACAAGTTGGGAA+GGG 0.649759 6.1:-255428 None:intergenic
TTGAGGTAACCACAAAGGCA+TGG 0.650224 6.1:-255338 None:intergenic
GATTCTTGAGGTAACCACAA+AGG 0.669776 6.1:-255343 None:intergenic
GTTGTGATTGATCCTAAACA+AGG 0.684294 6.1:-255266 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAGCAGTATATTTACTCTCC+TGG + chr6.1:255374-255393 MS.gene82783:CDS 35.0%
ATGAAGAAACACTCTAGCTA+TGG + chr6.1:255149-255168 MS.gene82783:CDS 35.0%
GAGAGTAAATATACTGCTTG+AGG - chr6.1:255373-255392 None:intergenic 35.0%
GTTGTGATTGATCCTAAACA+AGG - chr6.1:255269-255288 None:intergenic 35.0%
TAAATATACTGCTTGAGGCT+TGG - chr6.1:255368-255387 None:intergenic 35.0%
TGGTATTGTTTATGCAGTAG+TGG + chr6.1:255169-255188 MS.gene82783:CDS 35.0%
AAACAAGGACTGAACTCTAC+AGG - chr6.1:255254-255273 None:intergenic 40.0%
AACAAGGACTGAACTCTACA+GGG - chr6.1:255253-255272 None:intergenic 40.0%
GATTCTTGAGGTAACCACAA+AGG - chr6.1:255346-255365 None:intergenic 40.0%
TGTTTATGCAGTAGTGGTTG+TGG + chr6.1:255175-255194 MS.gene82783:CDS 40.0%
! GTAGAGTTCAGTCCTTGTTT+AGG + chr6.1:255254-255273 MS.gene82783:CDS 40.0%
! GTTGTGGCAGTAGTGTTTTT+GGG + chr6.1:255191-255210 MS.gene82783:CDS 40.0%
!! TTGTGGCAGTAGTGTTTTTG+GGG + chr6.1:255192-255211 MS.gene82783:CDS 40.0%
GAAGGAGACTTACATGGCAA+AGG + chr6.1:255214-255233 MS.gene82783:CDS 45.0%
GTCTCTGCTCTCTTAACTTC+TGG - chr6.1:255319-255338 None:intergenic 45.0%
TTCTGGCAACAAGCACTTGT+TGG - chr6.1:255302-255321 None:intergenic 45.0%
TTGAGGTAACCACAAAGGCA+TGG - chr6.1:255341-255360 None:intergenic 45.0%
TTTGGGGAAGGAGACTTACA+TGG + chr6.1:255208-255227 MS.gene82783:CDS 45.0%
! AAGGGTTACTAGCTCTTGTG+GGG + chr6.1:255403-255422 MS.gene82783:CDS 45.0%
! AGAAGGGTTACTAGCTCTTG+TGG + chr6.1:255401-255420 MS.gene82783:CDS 45.0%
! GAAGGGTTACTAGCTCTTGT+GGG + chr6.1:255402-255421 MS.gene82783:CDS 45.0%
! GGTTGTGGCAGTAGTGTTTT+TGG + chr6.1:255190-255209 MS.gene82783:CDS 45.0%
! TTACTCTCCTGGTGCTAGAA+GGG + chr6.1:255385-255404 MS.gene82783:CDS 45.0%
! TTTACTCTCCTGGTGCTAGA+AGG + chr6.1:255384-255403 MS.gene82783:CDS 45.0%
CTAGTAACCCTTCTAGCACC+AGG - chr6.1:255395-255414 None:intergenic 50.0%
GCTTGAGGCTTGGATTCTTG+AGG - chr6.1:255358-255377 None:intergenic 50.0%
! TGGCAACAAGCACTTGTTGG+TGG - chr6.1:255299-255318 None:intergenic 50.0%
!!! GGCAGTAGTGTTTTTGGGGA+AGG + chr6.1:255196-255215 MS.gene82783:CDS 50.0%
GAGCAGAGACCATGCCTTTG+TGG + chr6.1:255329-255348 MS.gene82783:CDS 55.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 255149 255445 255149 ID=MS.gene82783
chr6.1 mRNA 255149 255445 255149 ID=MS.gene82783.t1;Parent=MS.gene82783
chr6.1 exon 255149 255445 255149 ID=MS.gene82783.t1.exon1;Parent=MS.gene82783.t1
chr6.1 CDS 255149 255445 255149 ID=cds.MS.gene82783.t1;Parent=MS.gene82783.t1
Gene Sequence

>MS.gene82783

ATGAAGAAACACTCTAGCTATGGTATTGTTTATGCAGTAGTGGTTGTGGCAGTAGTGTTTTTGGGGAAGGAGACTTACATGGCAAAGGCATTGACTTGTAGCCCTGTAGAGTTCAGTCCTTGTTTAGGATCAATCACAACTTCTTCTCCACCAACAAGTGCTTGTTGCCAGAAGTTAAGAGAGCAGAGACCATGCCTTTGTGGTTACCTCAAGAATCCAAGCCTCAAGCAGTATATTTACTCTCCTGGTGCTAGAAGGGTTACTAGCTCTTGTGGGGTTCCCTTCCCAACTTGTTAG
Protein sequence

>MS.gene82783.t1

MKKHSSYGIVYAVVVVAVVFLGKETYMAKALTCSPVEFSPCLGSITTSSPPTSACCQKLREQRPCLCGYLKNPSLKQYIYSPGARRVTSSCGVPFPTC