AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053094


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053094.t1 MTR_7g007440 94.898 98 5 0 1 98 1 98 3.54e-56 169
MS.gene053094.t1 MTR_7g082640 53.933 89 41 0 10 98 6 94 2.99e-35 115
MS.gene053094.t1 MTR_6g082480 49.383 81 41 0 18 98 12 92 4.97e-29 100
MS.gene053094.t1 MTR_1g109650 42.222 90 52 0 9 98 3 92 1.54e-26 94.0
MS.gene053094.t1 MTR_7g082590 42.045 88 51 0 10 97 6 93 1.81e-22 83.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053094.t1 AT3G18280 49.438 89 42 1 10 98 35 120 2.35e-30 104
MS.gene053094.t1 AT3G18280 49.438 89 42 1 10 98 11 96 4.03e-30 103
MS.gene053094.t1 AT1G48750 55.556 72 32 0 27 98 23 94 1.41e-28 99.4
MS.gene053094.t1 AT1G73780 34.694 98 64 0 1 98 1 98 2.67e-22 83.6
MS.gene053094.t1 AT1G66850 50.725 69 34 0 30 98 34 102 1.95e-21 81.3
MS.gene053094.t1 AT1G43666 46.154 91 43 3 8 98 11 95 3.68e-20 77.8
MS.gene053094.t1 AT5G38170 45.588 68 37 0 31 98 36 103 4.46e-19 75.5
MS.gene053094.t1 AT5G38195 47.761 67 35 0 32 98 29 95 8.84e-19 74.3
MS.gene053094.t1 AT1G43667 35.000 100 61 2 1 98 1 98 1.80e-18 73.6
MS.gene053094.t1 AT5G38160 40.260 77 42 1 22 98 31 103 9.40e-18 72.0
MS.gene053094.t1 AT5G38180 36.000 100 57 2 1 98 1 95 1.05e-17 71.6
MS.gene053094.t1 AT2G14846 45.161 62 34 0 37 98 38 99 1.26e-14 63.9
MS.gene053094.t1 AT1G43665 36.250 80 45 2 17 96 22 95 2.21e-14 63.9
MS.gene053094.t1 AT1G07747 40.909 66 37 1 33 98 29 92 2.22e-13 60.8
MS.gene053094.t1 AT5G38197 34.694 98 62 2 1 98 1 96 4.46e-13 60.1
MS.gene053094.t1 AT3G57310 36.364 66 42 0 33 98 38 103 1.02e-12 59.3
MS.gene053094.t1 AT4G12825 34.066 91 50 2 16 98 7 95 1.62e-12 58.5

Find 28 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAGAGTTCAGTCCTTGTTT+AGG 0.334416 6.2:+250190 MS.gene053094:CDS
GTCTCTGCTCTCTTAACTTC+TGG 0.337118 6.2:-250252 None:intergenic
TTTACTCTCCTGGTGCTAGA+AGG 0.339135 6.2:+250320 MS.gene053094:CDS
GAGCAGAGACCATGCCTTTG+TGG 0.411218 6.2:+250265 MS.gene053094:CDS
GAAGGGTTACTAGCTCTTGT+GGG 0.413579 6.2:+250338 MS.gene053094:CDS
TTCTGGCAACAAGCACTTGT+TGG 0.433827 6.2:-250235 None:intergenic
TAAATATACTGCTTGAGGCT+TGG 0.456606 6.2:-250301 None:intergenic
ATGATTGATGGCTAACAAGT+TGG 0.458305 6.2:-250370 None:intergenic
AAACAAGGACTGAACTCTAC+AGG 0.466330 6.2:-250187 None:intergenic
TGATTGATGGCTAACAAGTT+GGG 0.497570 6.2:-250369 None:intergenic
TGGCAACAAGCACTTGTTGG+TGG 0.497936 6.2:-250232 None:intergenic
AGAAGGGTTACTAGCTCTTG+TGG 0.498825 6.2:+250337 MS.gene053094:CDS
TGTTTATGCAGTAGTGGTTG+TGG 0.508459 6.2:+250111 MS.gene053094:CDS
AAGCAGTATATTTACTCTCC+TGG 0.517554 6.2:+250310 MS.gene053094:CDS
TGGTATTGTTTATGCAGTAG+TGG 0.524181 6.2:+250105 MS.gene053094:CDS
CTAGTAACCCTTCTAGCACC+AGG 0.531212 6.2:-250328 None:intergenic
TTACTCTCCTGGTGCTAGAA+GGG 0.532631 6.2:+250321 MS.gene053094:CDS
GCTTGAGGCTTGGATTCTTG+AGG 0.548134 6.2:-250291 None:intergenic
ATGAAGAAACACTCTAGCTA+TGG 0.561126 6.2:+250085 MS.gene053094:CDS
TGATGGCTAACAAGTTGGGA+AGG 0.576643 6.2:-250365 None:intergenic
GAGAGTAAATATACTGCTTG+AGG 0.582416 6.2:-250306 None:intergenic
AAGGGTTACTAGCTCTTGTG+GGG 0.597115 6.2:+250339 MS.gene053094:CDS
GGAGGAGACTTACATGGCAA+AGG 0.624840 6.2:+250150 MS.gene053094:CDS
AACAAGGACTGAACTCTACA+GGG 0.629235 6.2:-250186 None:intergenic
GATGGCTAACAAGTTGGGAA+GGG 0.649759 6.2:-250364 None:intergenic
TTGAGGTAACCACAAAGGCA+TGG 0.650224 6.2:-250274 None:intergenic
GATTCTTGAGGTAACCACAA+AGG 0.669776 6.2:-250279 None:intergenic
GTTGTGATTGATCCTAAACA+AGG 0.684294 6.2:-250202 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAGCAGTATATTTACTCTCC+TGG + chr6.2:250310-250329 MS.gene053094:CDS 35.0%
ATGAAGAAACACTCTAGCTA+TGG + chr6.2:250085-250104 MS.gene053094:CDS 35.0%
GAGAGTAAATATACTGCTTG+AGG - chr6.2:250309-250328 None:intergenic 35.0%
GTTGTGATTGATCCTAAACA+AGG - chr6.2:250205-250224 None:intergenic 35.0%
TAAATATACTGCTTGAGGCT+TGG - chr6.2:250304-250323 None:intergenic 35.0%
TGGTATTGTTTATGCAGTAG+TGG + chr6.2:250105-250124 MS.gene053094:CDS 35.0%
! GTTGTGGCAATAGTGTTTTT+GGG + chr6.2:250127-250146 MS.gene053094:CDS 35.0%
!! TTGTGGCAATAGTGTTTTTG+GGG + chr6.2:250128-250147 MS.gene053094:CDS 35.0%
AAACAAGGACTGAACTCTAC+AGG - chr6.2:250190-250209 None:intergenic 40.0%
AACAAGGACTGAACTCTACA+GGG - chr6.2:250189-250208 None:intergenic 40.0%
GATTCTTGAGGTAACCACAA+AGG - chr6.2:250282-250301 None:intergenic 40.0%
TGTTTATGCAGTAGTGGTTG+TGG + chr6.2:250111-250130 MS.gene053094:CDS 40.0%
! GGTTGTGGCAATAGTGTTTT+TGG + chr6.2:250126-250145 MS.gene053094:CDS 40.0%
! GTAGAGTTCAGTCCTTGTTT+AGG + chr6.2:250190-250209 MS.gene053094:CDS 40.0%
!!! TGTGGCAATAGTGTTTTTGG+GGG + chr6.2:250129-250148 MS.gene053094:CDS 40.0%
GTCTCTGCTCTCTTAACTTC+TGG - chr6.2:250255-250274 None:intergenic 45.0%
TTCTGGCAACAAGCACTTGT+TGG - chr6.2:250238-250257 None:intergenic 45.0%
TTGAGGTAACCACAAAGGCA+TGG - chr6.2:250277-250296 None:intergenic 45.0%
! AAGGGTTACTAGCTCTTGTG+GGG + chr6.2:250339-250358 MS.gene053094:CDS 45.0%
! AGAAGGGTTACTAGCTCTTG+TGG + chr6.2:250337-250356 MS.gene053094:CDS 45.0%
! GAAGGGTTACTAGCTCTTGT+GGG + chr6.2:250338-250357 MS.gene053094:CDS 45.0%
! TTACTCTCCTGGTGCTAGAA+GGG + chr6.2:250321-250340 MS.gene053094:CDS 45.0%
! TTTACTCTCCTGGTGCTAGA+AGG + chr6.2:250320-250339 MS.gene053094:CDS 45.0%
CTAGTAACCCTTCTAGCACC+AGG - chr6.2:250331-250350 None:intergenic 50.0%
GCTTGAGGCTTGGATTCTTG+AGG - chr6.2:250294-250313 None:intergenic 50.0%
GGAGGAGACTTACATGGCAA+AGG + chr6.2:250150-250169 MS.gene053094:CDS 50.0%
TTTGGGGGAGGAGACTTACA+TGG + chr6.2:250144-250163 MS.gene053094:CDS 50.0%
! TGGCAACAAGCACTTGTTGG+TGG - chr6.2:250235-250254 None:intergenic 50.0%
!!! GGCAATAGTGTTTTTGGGGG+AGG + chr6.2:250132-250151 MS.gene053094:CDS 50.0%
GAGCAGAGACCATGCCTTTG+TGG + chr6.2:250265-250284 MS.gene053094:CDS 55.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 250085 250381 250085 ID=MS.gene053094
chr6.2 mRNA 250085 250381 250085 ID=MS.gene053094.t1;Parent=MS.gene053094
chr6.2 exon 250085 250381 250085 ID=MS.gene053094.t1.exon1;Parent=MS.gene053094.t1
chr6.2 CDS 250085 250381 250085 ID=cds.MS.gene053094.t1;Parent=MS.gene053094.t1
Gene Sequence

>MS.gene053094

ATGAAGAAACACTCTAGCTATGGTATTGTTTATGCAGTAGTGGTTGTGGCAATAGTGTTTTTGGGGGAGGAGACTTACATGGCAAAGGCATTGACTTGTAGCCCTGTAGAGTTCAGTCCTTGTTTAGGATCAATCACAACTTCTTCTCCACCAACAAGTGCTTGTTGCCAGAAGTTAAGAGAGCAGAGACCATGCCTTTGTGGTTACCTCAAGAATCCAAGCCTCAAGCAGTATATTTACTCTCCTGGTGCTAGAAGGGTTACTAGCTCTTGTGGGGTTCCCTTCCCAACTTGTTAG

Protein sequence

>MS.gene053094.t1

MKKHSSYGIVYAVVVVAIVFLGEETYMAKALTCSPVEFSPCLGSITTSSPPTSACCQKLREQRPCLCGYLKNPSLKQYIYSPGARRVTSSCGVPFPTC