AlfalfaGEDB | MS.gene82985

KEGG: K23154
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82985.t1	RHN62020.1	96.4	110	4	0	1	110	1	110	2.30E-47	198

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82985.t1	I1L3T1	55.5	110	48	1	2	110	4	113	2.1e-25	116.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82985.t1	G7JGC4	96.4	110	4	0	1	110	1	110	1.7e-47	198.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055942	MS.gene82985	0.801993	6.93E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82985.t1	MTR_4g082810	96.364	110	4	0	1	110	1	110	2.61e-69	213
MS.gene82985.t1	MTR_4g082820	86.364	110	13	1	1	110	1	108	1.53e-57	184
MS.gene82985.t1	MTR_5g043630	58.242	91	34	1	6	92	7	97	4.10e-30	107
MS.gene82985.t1	MTR_6g078320	52.294	109	46	4	2	104	4	112	5.55e-27	103

Find 34 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTGGTGGTGGAAGGTTAAA+TGG	0.260928	4.2:-26919389	None:intergenic
GGGTGGAGTCGCGAAGGGTT+TGG	0.376572	4.2:-26919454	None:intergenic
GAAGAAGAAGGTGGTGGTGG+TGG	0.427434	4.2:-26919401	None:intergenic
ATGGAACACGGTTAACTTGA+GGG	0.435621	4.2:-26919370	None:intergenic
ATGGAAGAAGAAGGTGGTGG+TGG	0.475612	4.2:-26919404	None:intergenic
TATGTTTCCAAGTGCAGGAA+TGG	0.479703	4.2:+26919217	MS.gene82985:CDS
GAAAGTGAAGAGATCAAAGA+AGG	0.488234	4.2:-26919485	None:intergenic
AAGAGATCAAAGAAGGAAGA+AGG	0.494956	4.2:-26919478	None:intergenic
GTTGCTATGTTTCCAAGTGC+AGG	0.495580	4.2:+26919212	MS.gene82985:CDS
AAGAAGAGTGGAAATGATCA+AGG	0.496372	4.2:-26919346	None:intergenic
AAGAAGGTGGTGGTGGTGGA+AGG	0.509600	4.2:-26919397	None:intergenic
ATGTTTCCAAGTGCAGGAAT+GGG	0.522998	4.2:+26919218	MS.gene82985:CDS
GCAAGAGAGACAGTGGGAAG+AGG	0.523325	4.2:-26919311	None:intergenic
AGTCGCGAAGGGTTTGGACT+CGG	0.528692	4.2:-26919448	None:intergenic
GAAGGTGGGTGGAGTCGCGA+AGG	0.532402	4.2:-26919460	None:intergenic
AGATCAAAGAAGGAAGAAGG+TGG	0.536328	4.2:-26919475	None:intergenic
CAAAGAAGGAAGAAGGTGGG+TGG	0.557011	4.2:-26919471	None:intergenic
GATCAAAGAAGGAAGAAGGT+GGG	0.564967	4.2:-26919474	None:intergenic
AATGGAACACGGTTAACTTG+AGG	0.565772	4.2:-26919371	None:intergenic
AGGTGACCCATTCCTGCACT+TGG	0.577693	4.2:-26919224	None:intergenic
ATTGTTGTTGAGAAGCAAAG+AGG	0.588370	4.2:-26919267	None:intergenic
TCAACGGAGATGGAAGAAGA+AGG	0.609413	4.2:-26919413	None:intergenic
CGCGAAGGGTTTGGACTCGG+AGG	0.626128	4.2:-26919445	None:intergenic
AAAGAGGCTAGTCTGAGGAA+TGG	0.626395	4.2:-26919251	None:intergenic
TAACTTGAGGGAAAGAAGAG+TGG	0.631553	4.2:-26919358	None:intergenic
TGATTCTGCAAGAGAGACAG+TGG	0.636474	4.2:-26919318	None:intergenic
GAAGCAAAGAGGCTAGTCTG+AGG	0.645661	4.2:-26919256	None:intergenic
GAGATGGAAGAAGAAGGTGG+TGG	0.647906	4.2:-26919407	None:intergenic
AAGGTGGGTGGAGTCGCGAA+GGG	0.650363	4.2:-26919459	None:intergenic
CACTTGGAAACATAGCAACA+TGG	0.656198	4.2:-26919208	None:intergenic
GATTCTGCAAGAGAGACAGT+GGG	0.694334	4.2:-26919317	None:intergenic
ACGGAGATGGAAGAAGAAGG+TGG	0.698362	4.2:-26919410	None:intergenic
CTAGTCTGAGGAATGGTGTG+AGG	0.710246	4.2:-26919244	None:intergenic
GTGGAAGGTTAAATGGAACA+CGG	0.714342	4.2:-26919382	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGAAGAGTGGAAATGATCA+AGG	-	chr4.2:26919349-26919368	None:intergenic	35.0%
	AAGAGATCAAAGAAGGAAGA+AGG	-	chr4.2:26919481-26919500	None:intergenic	35.0%
	GAAAGTGAAGAGATCAAAGA+AGG	-	chr4.2:26919488-26919507	None:intergenic	35.0%
!	ATTGTTGTTGAGAAGCAAAG+AGG	-	chr4.2:26919270-26919289	None:intergenic	35.0%
	AGATCAAAGAAGGAAGAAGG+TGG	-	chr4.2:26919478-26919497	None:intergenic	40.0%
	ATGTTTCCAAGTGCAGGAAT+GGG	+	chr4.2:26919218-26919237	MS.gene82985:CDS	40.0%
	CACTTGGAAACATAGCAACA+TGG	-	chr4.2:26919211-26919230	None:intergenic	40.0%
	GATCAAAGAAGGAAGAAGGT+GGG	-	chr4.2:26919477-26919496	None:intergenic	40.0%
	GTGGAAGGTTAAATGGAACA+CGG	-	chr4.2:26919385-26919404	None:intergenic	40.0%
	TAACTTGAGGGAAAGAAGAG+TGG	-	chr4.2:26919361-26919380	None:intergenic	40.0%
	TATGTTTCCAAGTGCAGGAA+TGG	+	chr4.2:26919217-26919236	MS.gene82985:CDS	40.0%
	TTTGGACTCGGAGGAAAAAA+GGG	-	chr4.2:26919439-26919458	None:intergenic	40.0%
!	AATGGAACACGGTTAACTTG+AGG	-	chr4.2:26919374-26919393	None:intergenic	40.0%
!	ATGGAACACGGTTAACTTGA+GGG	-	chr4.2:26919373-26919392	None:intergenic	40.0%
	GAAAAAAGGGTCAACGGAGA+TGG	-	chr4.2:26919426-26919445	None:intergenic	45.0%
	GATTCTGCAAGAGAGACAGT+GGG	-	chr4.2:26919320-26919339	None:intergenic	45.0%
	GTTGCTATGTTTCCAAGTGC+AGG	+	chr4.2:26919212-26919231	MS.gene82985:CDS	45.0%
	GTTTGGACTCGGAGGAAAAA+AGG	-	chr4.2:26919440-26919459	None:intergenic	45.0%
	TCAACGGAGATGGAAGAAGA+AGG	-	chr4.2:26919416-26919435	None:intergenic	45.0%
	TCGGAGGAAAAAAGGGTCAA+CGG	-	chr4.2:26919432-26919451	None:intergenic	45.0%
	TGATTCTGCAAGAGAGACAG+TGG	-	chr4.2:26919321-26919340	None:intergenic	45.0%
!!	AAAGAGGCTAGTCTGAGGAA+TGG	-	chr4.2:26919254-26919273	None:intergenic	45.0%
	ACGGAGATGGAAGAAGAAGG+TGG	-	chr4.2:26919413-26919432	None:intergenic	50.0%
	CAAAGAAGGAAGAAGGTGGG+TGG	-	chr4.2:26919474-26919493	None:intergenic	50.0%
	CTAGTCTGAGGAATGGTGTG+AGG	-	chr4.2:26919247-26919266	None:intergenic	50.0%
	GAGATGGAAGAAGAAGGTGG+TGG	-	chr4.2:26919410-26919429	None:intergenic	50.0%
!	ATGGAAGAAGAAGGTGGTGG+TGG	-	chr4.2:26919407-26919426	None:intergenic	50.0%
!!	GAAGCAAAGAGGCTAGTCTG+AGG	-	chr4.2:26919259-26919278	None:intergenic	50.0%
!!	GGTGGTGGTGGAAGGTTAAA+TGG	-	chr4.2:26919392-26919411	None:intergenic	50.0%
	AGGTGACCCATTCCTGCACT+TGG	-	chr4.2:26919227-26919246	None:intergenic	55.0%
	AGTCGCGAAGGGTTTGGACT+CGG	-	chr4.2:26919451-26919470	None:intergenic	55.0%
	GCAAGAGAGACAGTGGGAAG+AGG	-	chr4.2:26919314-26919333	None:intergenic	55.0%
!!	AAGAAGGTGGTGGTGGTGGA+AGG	-	chr4.2:26919400-26919419	None:intergenic	55.0%
!!	GAAGAAGAAGGTGGTGGTGG+TGG	-	chr4.2:26919404-26919423	None:intergenic	55.0%
	AAGGTGGGTGGAGTCGCGAA+GGG	-	chr4.2:26919462-26919481	None:intergenic	60.0%
	CGCGAAGGGTTTGGACTCGG+AGG	-	chr4.2:26919448-26919467	None:intergenic	65.0%
	GAAGGTGGGTGGAGTCGCGA+AGG	-	chr4.2:26919463-26919482	None:intergenic	65.0%
	GGGTGGAGTCGCGAAGGGTT+TGG	-	chr4.2:26919457-26919476	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	26919191	26919520	26919191	ID=MS.gene82985
chr4.2	mRNA	26919191	26919520	26919191	ID=MS.gene82985.t1;Parent=MS.gene82985
chr4.2	exon	26919191	26919520	26919191	ID=MS.gene82985.t1.exon1;Parent=MS.gene82985.t1
chr4.2	CDS	26919191	26919520	26919191	ID=cds.MS.gene82985.t1;Parent=MS.gene82985.t1

Gene Sequence	>MS.gene82985 ATGTCTGATGAAGCTGTCCATGTTGCTATGTTTCCAAGTGCAGGAATGGGTCACCTCACACCATTCCTCAGACTAGCCTCTTTGCTTCTCAACAACAATTGCAAAGTCACTCTCATCACTCCTCTTCCCACTGTCTCTCTTGCAGAATCACAACTCCTTGATCATTTCCACTCTTCTTTCCCTCAAGTTAACCGTGTTCCATTTAACCTTCCACCACCACCACCTTCTTCTTCCATCTCCGTTGACCCTTTTTTCCTCCGAGTCCAAACCCTTCGCGACTCCACCCACCTTCTTCCTTCTTTGATCTCTTCACTTTCACCACCCATCACT
Protein sequence	>MS.gene82985.t1 MSDEAVHVAMFPSAGMGHLTPFLRLASLLLNNNCKVTLITPLPTVSLAESQLLDHFHSSFPQVNRVPFNLPPPPPSSSISVDPFFLRVQTLRDSTHLLPSLISSLSPPIT

M. sativa cultivar XinJiangDaYe / MS.gene82985