Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83071.t1 | XP_024638642.1 | 93.8 | 128 | 8 | 0 | 1 | 128 | 1 | 128 | 2.20E-57 | 232.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83071.t1 | Q9CX48 | 55.9 | 34 | 15 | 0 | 86 | 119 | 20 | 53 | 5.8e-06 | 52.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83071.t1 | A0A396IFI3 | 93.8 | 128 | 8 | 0 | 1 | 128 | 1 | 128 | 1.6e-57 | 232.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene045256 | MS.gene83071 | PPI |
| MS.gene83071 | MS.gene066824 | PPI |
| MS.gene75592 | MS.gene83071 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83071.t1 | MTR_1g032030 | 91.045 | 67 | 5 | 1 | 18 | 83 | 15 | 81 | 2.86e-22 | 89.4 |
| MS.gene83071.t1 | MTR_1g032370 | 91.045 | 67 | 5 | 1 | 18 | 83 | 15 | 81 | 2.86e-22 | 89.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83071.t1 | AT5G49400 | 74.803 | 127 | 24 | 2 | 9 | 129 | 16 | 140 | 4.96e-48 | 159 |
Find 52 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTAGTCTGCTTCTTCTTC+TGG | 0.203184 | 1.2:+16878590 | None:intergenic |
| GACACATCCGGCCTCAATTT+AGG | 0.222584 | 1.2:+16878835 | None:intergenic |
| ATCAGATTATTCTTCTAGTT+CGG | 0.272097 | 1.2:-16878668 | MS.gene83071:CDS |
| TAGTAAGAAGGAAGAGATTA+AGG | 0.276310 | 1.2:-16879187 | MS.gene83071:CDS |
| ACACATCCGGCCTCAATTTA+GGG | 0.281606 | 1.2:+16878836 | None:intergenic |
| GGCCGCAGTTGCTGCTCTTT+CGG | 0.294122 | 1.2:+16879122 | None:intergenic |
| TCAGATTATTCTTCTAGTTC+GGG | 0.343157 | 1.2:-16878667 | MS.gene83071:CDS |
| CCATCGATAGTCGACATTGT+AGG | 0.393513 | 1.2:+16878964 | None:intergenic |
| GTCAAAGAGGAAGCATGATT+CGG | 0.395633 | 1.2:-16878728 | MS.gene83071:CDS |
| TCAAAGAGGAAGCATGATTC+GGG | 0.417245 | 1.2:-16878727 | MS.gene83071:CDS |
| CAGCATTAATTGCTGCGAAA+GGG | 0.433572 | 1.2:-16879161 | MS.gene83071:CDS |
| TGTGTCTGTGGATTATGATT+TGG | 0.452148 | 1.2:-16878818 | MS.gene83071:CDS |
| GCGCGTAATGTTAACGCATA+TGG | 0.458903 | 1.2:-16879099 | MS.gene83071:CDS |
| CAGAGGAAGAAAGGAGAAGG+AGG | 0.477099 | 1.2:-16878624 | MS.gene83071:CDS |
| ATGAGCGGGTTTATATTTCA+AGG | 0.478841 | 1.2:-16878885 | MS.gene83071:CDS |
| GCAGCATTAATTGCTGCGAA+AGG | 0.482444 | 1.2:-16879162 | MS.gene83071:CDS |
| CATGATTCGGGTGTTGATAG+TGG | 0.485011 | 1.2:-16878715 | MS.gene83071:CDS |
| CGTCTGAATCAGAGGAAGAA+AGG | 0.505020 | 1.2:-16878633 | MS.gene83071:CDS |
| AATGTTACCAGGGTGGGCAC+TGG | 0.514214 | 1.2:-16878924 | MS.gene83071:CDS |
| GAATATGTTGAGTAGTAAGA+AGG | 0.518238 | 1.2:-16879199 | None:intergenic |
| CAGTGGATCATCGTCTGCCA+CGG | 0.518690 | 1.2:-16878692 | MS.gene83071:CDS |
| TGCTACAGTGGTTGAAAGGA+AGG | 0.521953 | 1.2:-16878995 | MS.gene83071:CDS |
| GCATATGGTCAGAAGGAAGA+AGG | 0.525781 | 1.2:-16879084 | MS.gene83071:CDS |
| GCAGTGTCAGAAATGTTACC+AGG | 0.532627 | 1.2:-16878935 | MS.gene83071:CDS |
| TACTGAGAAAGCTGCTACAG+TGG | 0.535042 | 1.2:-16879007 | MS.gene83071:CDS |
| TCATAATCCACAGACACATC+CGG | 0.535556 | 1.2:+16878823 | None:intergenic |
| AGTGGATCATCGTCTGCCAC+GGG | 0.553302 | 1.2:-16878691 | MS.gene83071:CDS |
| AGAAGGGCCTAGCAGATGGC+AGG | 0.555494 | 1.2:-16879067 | MS.gene83071:CDS |
| TTAAGTTGCTGAGTCCTCGA+TGG | 0.557112 | 1.2:+16878862 | None:intergenic |
| CAGCTCATCGTCTGAATCAG+AGG | 0.559637 | 1.2:-16878641 | MS.gene83071:CDS |
| TCGGGTGTTGATAGTGGCAG+TGG | 0.566877 | 1.2:-16878709 | MS.gene83071:CDS |
| GTCAGAAATGTTACCAGGGT+GGG | 0.567291 | 1.2:-16878930 | MS.gene83071:CDS |
| ATTTCAATGTAAGAATGAGC+GGG | 0.569709 | 1.2:-16878899 | MS.gene83071:CDS |
| AGGAAGAAGGGCCTAGCAGA+TGG | 0.572751 | 1.2:-16879071 | MS.gene83071:CDS |
| AAGCTGCTACAGTGGTTGAA+AGG | 0.575356 | 1.2:-16878999 | MS.gene83071:CDS |
| AGCCGAAAGAGCAGCAACTG+CGG | 0.577539 | 1.2:-16879124 | MS.gene83071:CDS |
| TCGTGATGTGAAGGAGGAGA+AGG | 0.580022 | 1.2:-16878770 | MS.gene83071:CDS |
| TGTTAACGCATATGGTCAGA+AGG | 0.582981 | 1.2:-16879091 | MS.gene83071:CDS |
| TAATAATCATCGTGATGTGA+AGG | 0.585747 | 1.2:-16878779 | MS.gene83071:CDS |
| CATTTCAATGTAAGAATGAG+CGG | 0.604438 | 1.2:-16878900 | MS.gene83071:CDS |
| CAGACTAAGAAGAAGAGCAA+AGG | 0.608854 | 1.2:-16878577 | MS.gene83071:CDS |
| AATCAGAGGAAGAAAGGAGA+AGG | 0.616394 | 1.2:-16878627 | MS.gene83071:CDS |
| CATATGGTCAGAAGGAAGAA+GGG | 0.616780 | 1.2:-16879083 | MS.gene83071:CDS |
| TAATCATCGTGATGTGAAGG+AGG | 0.624901 | 1.2:-16878776 | MS.gene83071:CDS |
| CCTACAATGTCGACTATCGA+TGG | 0.625467 | 1.2:-16878964 | MS.gene83071:CDS |
| ATTGAGGCCGGATGTGTCTG+TGG | 0.648025 | 1.2:-16878830 | MS.gene83071:CDS |
| GAAATGTCCAGTGCCCACCC+TGG | 0.650305 | 1.2:+16878917 | None:intergenic |
| CAGTGTCAGAAATGTTACCA+GGG | 0.672372 | 1.2:-16878934 | MS.gene83071:CDS |
| TGTCAGAAATGTTACCAGGG+TGG | 0.676105 | 1.2:-16878931 | MS.gene83071:CDS |
| AGAAGAATAATCTGATCCCG+TGG | 0.687932 | 1.2:+16878675 | None:intergenic |
| TTTATATTTCAAGGCCATCG+AGG | 0.711495 | 1.2:-16878876 | MS.gene83071:CDS |
| ATATGCGTTAACATTACGCG+CGG | 0.742150 | 1.2:+16879101 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACTTAAAAACCCTAAATTG+AGG | - | chr1.2:16878901-16878920 | MS.gene83071:CDS | 25.0% |
| !! | ATCAGATTATTCTTCTAGTT+CGG | - | chr1.2:16879079-16879098 | MS.gene83071:CDS | 25.0% |
| ATTTCAATGTAAGAATGAGC+GGG | - | chr1.2:16878848-16878867 | MS.gene83071:CDS | 30.0% | |
| CATTTCAATGTAAGAATGAG+CGG | - | chr1.2:16878847-16878866 | MS.gene83071:CDS | 30.0% | |
| TAATAATCATCGTGATGTGA+AGG | - | chr1.2:16878968-16878987 | MS.gene83071:CDS | 30.0% | |
| TAGTAAGAAGGAAGAGATTA+AGG | - | chr1.2:16878560-16878579 | MS.gene83071:CDS | 30.0% | |
| !! | TCAGATTATTCTTCTAGTTC+GGG | - | chr1.2:16879080-16879099 | MS.gene83071:CDS | 30.0% |
| TAAAAACCCTAAATTGAGGC+CGG | - | chr1.2:16878905-16878924 | MS.gene83071:CDS | 35.0% | |
| TGAGTTCGAAAAAGTCAAAG+AGG | - | chr1.2:16879006-16879025 | MS.gene83071:CDS | 35.0% | |
| TTTATATTTCAAGGCCATCG+AGG | - | chr1.2:16878871-16878890 | MS.gene83071:CDS | 35.0% | |
| ! | ATGAGCGGGTTTATATTTCA+AGG | - | chr1.2:16878862-16878881 | MS.gene83071:CDS | 35.0% |
| ! | TGTGTCTGTGGATTATGATT+TGG | - | chr1.2:16878929-16878948 | MS.gene83071:CDS | 35.0% |
| AATCAGAGGAAGAAAGGAGA+AGG | - | chr1.2:16879120-16879139 | MS.gene83071:CDS | 40.0% | |
| AGAAGAATAATCTGATCCCG+TGG | + | chr1.2:16879075-16879094 | None:intergenic | 40.0% | |
| AGAAGAGCAAAGGAAAAAGC+AGG | - | chr1.2:16879180-16879199 | MS.gene83071:CDS | 40.0% | |
| ATATGCGTTAACATTACGCG+CGG | + | chr1.2:16878649-16878668 | None:intergenic | 40.0% | |
| CAGACTAAGAAGAAGAGCAA+AGG | - | chr1.2:16879170-16879189 | MS.gene83071:CDS | 40.0% | |
| CAGCATTAATTGCTGCGAAA+GGG | - | chr1.2:16878586-16878605 | MS.gene83071:CDS | 40.0% | |
| CAGTGTCAGAAATGTTACCA+GGG | - | chr1.2:16878813-16878832 | MS.gene83071:CDS | 40.0% | |
| CATATGGTCAGAAGGAAGAA+GGG | - | chr1.2:16878664-16878683 | MS.gene83071:CDS | 40.0% | |
| TAATCATCGTGATGTGAAGG+AGG | - | chr1.2:16878971-16878990 | MS.gene83071:CDS | 40.0% | |
| TCATAATCCACAGACACATC+CGG | + | chr1.2:16878927-16878946 | None:intergenic | 40.0% | |
| TCTTAGTCTGCTTCTTCTTC+TGG | + | chr1.2:16879160-16879179 | None:intergenic | 40.0% | |
| TGTTAACGCATATGGTCAGA+AGG | - | chr1.2:16878656-16878675 | MS.gene83071:CDS | 40.0% | |
| ! | GTCAAAGAGGAAGCATGATT+CGG | - | chr1.2:16879019-16879038 | MS.gene83071:CDS | 40.0% |
| ! | TCAAAGAGGAAGCATGATTC+GGG | - | chr1.2:16879020-16879039 | MS.gene83071:CDS | 40.0% |
| AAGCTGCTACAGTGGTTGAA+AGG | - | chr1.2:16878748-16878767 | MS.gene83071:CDS | 45.0% | |
| ACACATCCGGCCTCAATTTA+GGG | + | chr1.2:16878914-16878933 | None:intergenic | 45.0% | |
| AGAGCAAAGGAAAAAGCAGG+AGG | - | chr1.2:16879183-16879202 | MS.gene83071:CDS | 45.0% | |
| CCATCGATAGTCGACATTGT+AGG | + | chr1.2:16878786-16878805 | None:intergenic | 45.0% | |
| CGTCTGAATCAGAGGAAGAA+AGG | - | chr1.2:16879114-16879133 | MS.gene83071:CDS | 45.0% | |
| GCAGCATTAATTGCTGCGAA+AGG | - | chr1.2:16878585-16878604 | MS.gene83071:CDS | 45.0% | |
| GCAGTGTCAGAAATGTTACC+AGG | - | chr1.2:16878812-16878831 | MS.gene83071:CDS | 45.0% | |
| GCATATGGTCAGAAGGAAGA+AGG | - | chr1.2:16878663-16878682 | MS.gene83071:CDS | 45.0% | |
| GCGCGTAATGTTAACGCATA+TGG | - | chr1.2:16878648-16878667 | MS.gene83071:CDS | 45.0% | |
| GTCAGAAATGTTACCAGGGT+GGG | - | chr1.2:16878817-16878836 | MS.gene83071:CDS | 45.0% | |
| TACTGAGAAAGCTGCTACAG+TGG | - | chr1.2:16878740-16878759 | MS.gene83071:CDS | 45.0% | |
| TAGCAGATGGCAGGAAAAAC+AGG | - | chr1.2:16878689-16878708 | MS.gene83071:CDS | 45.0% | |
| TGCTACAGTGGTTGAAAGGA+AGG | - | chr1.2:16878752-16878771 | MS.gene83071:CDS | 45.0% | |
| TGTCAGAAATGTTACCAGGG+TGG | - | chr1.2:16878816-16878835 | MS.gene83071:CDS | 45.0% | |
| ! | CCTACAATGTCGACTATCGA+TGG | - | chr1.2:16878783-16878802 | MS.gene83071:CDS | 45.0% |
| ! | TGTTTTTCCTGCCATCTGCT+AGG | + | chr1.2:16878690-16878709 | None:intergenic | 45.0% |
| ! | TTAAGTTGCTGAGTCCTCGA+TGG | + | chr1.2:16878888-16878907 | None:intergenic | 45.0% |
| !! | CATGATTCGGGTGTTGATAG+TGG | - | chr1.2:16879032-16879051 | MS.gene83071:CDS | 45.0% |
| AGGAAAAACAGGAGGCGAAG+AGG | - | chr1.2:16878700-16878719 | MS.gene83071:CDS | 50.0% | |
| CAGAGGAAGAAAGGAGAAGG+AGG | - | chr1.2:16879123-16879142 | MS.gene83071:CDS | 50.0% | |
| CAGATGGCAGGAAAAACAGG+AGG | - | chr1.2:16878692-16878711 | MS.gene83071:CDS | 50.0% | |
| CAGCTCATCGTCTGAATCAG+AGG | - | chr1.2:16879106-16879125 | MS.gene83071:CDS | 50.0% | |
| GACACATCCGGCCTCAATTT+AGG | + | chr1.2:16878915-16878934 | None:intergenic | 50.0% | |
| TCGTGATGTGAAGGAGGAGA+AGG | - | chr1.2:16878977-16878996 | MS.gene83071:CDS | 50.0% | |
| AATGTTACCAGGGTGGGCAC+TGG | - | chr1.2:16878823-16878842 | MS.gene83071:CDS | 55.0% | |
| AGCCGAAAGAGCAGCAACTG+CGG | - | chr1.2:16878623-16878642 | MS.gene83071:CDS | 55.0% | |
| AGGAAGAAGGGCCTAGCAGA+TGG | - | chr1.2:16878676-16878695 | MS.gene83071:CDS | 55.0% | |
| AGTGGATCATCGTCTGCCAC+GGG | - | chr1.2:16879056-16879075 | MS.gene83071:CDS | 55.0% | |
| ATTGAGGCCGGATGTGTCTG+TGG | - | chr1.2:16878917-16878936 | MS.gene83071:CDS | 55.0% | |
| CAGTGGATCATCGTCTGCCA+CGG | - | chr1.2:16879055-16879074 | MS.gene83071:CDS | 55.0% | |
| !! | TCGGGTGTTGATAGTGGCAG+TGG | - | chr1.2:16879038-16879057 | MS.gene83071:CDS | 55.0% |
| AGAAGGGCCTAGCAGATGGC+AGG | - | chr1.2:16878680-16878699 | MS.gene83071:CDS | 60.0% | |
| GAAATGTCCAGTGCCCACCC+TGG | + | chr1.2:16878833-16878852 | None:intergenic | 60.0% | |
| GGCCGCAGTTGCTGCTCTTT+CGG | + | chr1.2:16878628-16878647 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 16878552 | 16879217 | 16878552 | ID=MS.gene83071 |
| chr1.2 | mRNA | 16878552 | 16879217 | 16878552 | ID=MS.gene83071.t1;Parent=MS.gene83071 |
| chr1.2 | exon | 16878552 | 16879217 | 16878552 | ID=MS.gene83071.t1.exon1;Parent=MS.gene83071.t1 |
| chr1.2 | CDS | 16878552 | 16879217 | 16878552 | ID=cds.MS.gene83071.t1;Parent=MS.gene83071.t1 |
| Gene Sequence |
| Protein sequence |
