AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene84220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84220.t1 MTR_3g466740 47.177 496 238 9 1 494 1 474 5.48e-144 421
MS.gene84220.t1 MTR_0067s0010 47.581 496 228 12 1 493 14 480 1.17e-141 416
MS.gene84220.t1 MTR_7g068160 36.449 535 275 16 11 494 17 537 2.21e-97 304
MS.gene84220.t1 MTR_7g067750 36.502 526 274 18 10 494 43 549 4.21e-95 298
MS.gene84220.t1 MTR_7g067970 35.359 543 290 13 5 494 4 538 9.05e-89 282
MS.gene84220.t1 MTR_7g067880 35.328 518 259 17 17 480 16 511 2.64e-86 275
MS.gene84220.t1 MTR_7g067940 33.818 550 278 13 11 494 10 539 7.04e-83 266
MS.gene84220.t1 MTR_7g067300 34.274 496 261 16 28 494 20 479 1.39e-72 238
MS.gene84220.t1 MTR_7g067600 31.627 547 293 19 5 494 2 524 1.55e-69 231
MS.gene84220.t1 MTR_1g040690 30.664 512 265 16 18 490 15 475 1.14e-61 209
MS.gene84220.t1 MTR_7g068070 46.667 180 92 3 10 187 9 186 1.56e-43 162
MS.gene84220.t1 MTR_7g068070 31.633 294 157 6 229 490 260 541 2.92e-33 132
MS.gene84220.t1 MTR_7g067990 32.737 391 213 15 138 494 223 597 8.48e-42 157
MS.gene84220.t1 MTR_7g067990 45.333 150 75 4 9 153 3 150 1.89e-28 119
MS.gene84220.t1 MTR_4g015410 30.260 423 189 15 92 486 25 369 2.60e-37 141
MS.gene84220.t1 MTR_7g067660 38.462 195 103 5 310 494 195 382 4.15e-36 138
MS.gene84220.t1 MTR_7g067660 41.143 175 92 5 17 182 3 175 3.07e-32 127
MS.gene84220.t1 MTR_1g038270 27.027 518 288 21 27 491 19 499 1.57e-34 135
MS.gene84220.t1 MTR_7g068150 29.487 390 180 15 11 354 6 346 1.32e-28 119
MS.gene84220.t1 MTR_7g068040 39.759 166 68 4 26 187 13 150 3.34e-26 104
MS.gene84220.t1 MTR_4g015450 32.500 240 120 9 269 486 251 470 1.90e-24 106
MS.gene84220.t1 MTR_5g086340 26.059 472 272 20 30 491 32 436 3.29e-21 96.7
MS.gene84220.t1 MTR_3g079390 23.077 468 286 17 35 489 45 451 1.54e-19 91.7
MS.gene84220.t1 MTR_6g011050 23.750 400 239 13 35 432 29 364 2.25e-17 84.3
MS.gene84220.t1 MTR_5g086230 31.098 164 100 4 28 184 26 183 3.26e-17 80.5
MS.gene84220.t1 MTR_6g010890 23.077 455 281 14 35 483 30 421 3.98e-16 80.9
MS.gene84220.t1 MTR_6g011060 23.058 412 236 12 35 432 30 374 5.62e-16 80.5
MS.gene84220.t1 MTR_6g009610 23.750 400 240 15 35 432 30 366 1.65e-15 79.0
MS.gene84220.t1 MTR_6g010920 23.153 406 239 14 35 435 30 367 1.87e-15 78.6
MS.gene84220.t1 MTR_2g041180 22.551 439 276 13 31 463 51 431 2.29e-15 79.0
MS.gene84220.t1 MTR_4g015290 37.984 129 58 4 18 140 16 128 2.96e-15 75.5
MS.gene84220.t1 MTR_6g010810 23.659 410 234 13 35 435 27 366 1.21e-14 76.3
MS.gene84220.t1 MTR_8g005285 22.831 438 266 16 19 413 25 433 3.14e-14 75.5
MS.gene84220.t1 MTR_3g107590 23.192 401 250 13 35 432 31 376 7.74e-14 73.9
MS.gene84220.t1 MTR_5g016570 29.452 146 94 4 31 173 102 241 2.02e-12 69.7
MS.gene84220.t1 MTR_2g036320 22.902 441 264 12 35 453 72 458 2.97e-12 69.3
MS.gene84220.t1 MTR_6g009170 24.808 391 215 16 35 413 30 353 7.58e-12 67.8
MS.gene84220.t1 MTR_7g068030 41.975 81 38 1 312 383 47 127 8.47e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84220.t1 AT3G50150 25.319 470 283 18 35 493 95 507 6.99e-28 117
MS.gene84220.t1 AT2G36430 25.000 440 262 13 8 439 26 405 2.00e-26 112
MS.gene84220.t1 AT3G47250 25.745 470 277 14 35 491 66 476 2.90e-26 112
MS.gene84220.t1 AT3G47250 25.745 470 277 14 35 491 66 476 2.90e-26 112
MS.gene84220.t1 AT3G47250 25.745 470 277 14 35 491 66 476 2.90e-26 112
MS.gene84220.t1 AT3G50160 25.696 467 263 18 35 493 109 499 1.26e-25 110
MS.gene84220.t1 AT3G50170 24.896 482 277 19 35 493 118 537 3.44e-25 109
MS.gene84220.t1 AT3G47200 24.893 466 282 15 35 490 51 458 1.19e-24 107
MS.gene84220.t1 AT3G47200 24.893 466 282 15 35 490 51 458 1.19e-24 107
MS.gene84220.t1 AT5G22540 23.844 411 254 12 35 439 36 393 6.98e-24 104
MS.gene84220.t1 AT4G31980 25.301 415 236 13 35 441 298 646 6.44e-23 102
MS.gene84220.t1 AT3G50140 24.894 470 292 18 35 493 118 537 1.44e-22 101
MS.gene84220.t1 AT3G47210 27.317 410 238 14 35 439 98 452 3.25e-22 99.8
MS.gene84220.t1 AT3G50180 25.370 473 270 17 35 493 183 586 6.32e-22 99.8
MS.gene84220.t1 AT3G44710 22.379 496 280 14 46 493 64 502 1.47e-19 92.0
MS.gene84220.t1 AT5G11290 24.668 377 224 10 36 411 39 356 4.27e-19 90.1
MS.gene84220.t1 AT5G22550 21.800 500 290 14 35 493 49 488 2.83e-18 87.8
MS.gene84220.t1 AT3G50150 24.671 304 187 11 35 328 95 366 6.91e-18 86.3
MS.gene84220.t1 AT3G50130 31.544 149 95 3 35 182 144 286 1.49e-16 82.8
MS.gene84220.t1 AT3G50140 25.329 304 192 10 35 328 118 396 2.37e-16 81.6
MS.gene84220.t1 AT3G50170 31.333 150 96 3 35 183 118 261 3.56e-16 80.9
MS.gene84220.t1 AT3G50120 30.667 150 97 3 35 183 108 251 5.01e-14 74.7
MS.gene84220.t1 AT3G50190 30.488 164 106 4 13 176 82 237 2.85e-13 71.6
MS.gene84220.t1 AT3G50190 30.488 164 106 4 13 176 82 237 3.28e-13 71.6
MS.gene84220.t1 AT3G50190 30.488 164 106 4 13 176 82 237 3.28e-13 71.6
MS.gene84220.t1 AT3G50190 30.488 164 106 4 13 176 82 237 3.38e-13 72.0
MS.gene84220.t1 AT5G22560 24.775 222 131 8 35 238 52 255 2.82e-12 69.3

Find 87 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGATTGAGTTGATCAATT+TGG 0.175142 7.1:-33688739 None:intergenic
GAGATTGAGTTGATCAATTT+GGG 0.233510 7.1:-33688738 None:intergenic
TATTGTTCGTGCAAGCTATA+TGG 0.294745 7.1:+33688382 MS.gene84220:CDS
CGAGTTATACGTAACGATTT+AGG 0.304073 7.1:+33689244 MS.gene84220:CDS
GTCTCTGTAATAACCAATTC+TGG 0.310249 7.1:-33689049 None:intergenic
GGTTTCTAATATAGCATAAT+AGG 0.328705 7.1:-33689383 None:intergenic
GACACTACTTGTTCCAGAAT+TGG 0.334562 7.1:+33689036 MS.gene84220:CDS
GCGACTAGGCTTTACGCATC+TGG 0.348043 7.1:+33688917 MS.gene84220:CDS
TCAAATCGTGGAGAATAATA+AGG 0.360628 7.1:+33688823 MS.gene84220:CDS
CAGAGACAATGGAATCAAAA+TGG 0.374706 7.1:+33689065 MS.gene84220:CDS
TGTCTATAATGATTTGCTTA+TGG 0.375743 7.1:+33688565 MS.gene84220:CDS
TGCTACGTTGATAACTCTTC+TGG 0.376103 7.1:+33689492 MS.gene84220:CDS
GTACCGATGGAGACAACTTT+TGG 0.394590 7.1:-33688224 None:intergenic
CTCCATCGGTACTCGATACA+TGG 0.396057 7.1:+33688235 MS.gene84220:CDS
AATGGCGGAATTTCATTGCT+TGG 0.400692 7.1:+33689083 MS.gene84220:CDS
TTTGATTTAAAATTCATCAA+TGG 0.403772 7.1:+33689013 MS.gene84220:CDS
CTAAAATCCTGCTGAAATAG+TGG 0.404718 7.1:-33689420 None:intergenic
CTTGGGAGCATCACAAGAAA+TGG 0.406524 7.1:+33689101 MS.gene84220:CDS
TTGATTTAAAATTCATCAAT+GGG 0.408863 7.1:+33689014 MS.gene84220:CDS
ATGTAGATCAATGGTATTGC+CGG 0.409307 7.1:-33688536 None:intergenic
AGCAAAGTGTAGGACTTACT+TGG 0.411269 7.1:-33689557 None:intergenic
GTATGATCTTGCTACAGTTA+TGG 0.412094 7.1:+33688424 MS.gene84220:CDS
TCAAAACTTGATATTTCAAC+TGG 0.416081 7.1:-33688326 None:intergenic
CGAAGTTCGTCTGGAACAAC+GGG 0.418614 7.1:-33688176 None:intergenic
CAACTCAATCTCAACTTCAA+TGG 0.419347 7.1:+33688749 MS.gene84220:CDS
ATGGCGGAATTTCATTGCTT+GGG 0.420231 7.1:+33689084 MS.gene84220:CDS
CGCCATTTGATCTCTTCCAT+TGG 0.424515 7.1:-33688275 None:intergenic
TACTTTGCCACTATTTCAGC+AGG 0.438865 7.1:+33689413 MS.gene84220:CDS
GGAACAACGGGAATGCTATA+TGG 0.443564 7.1:-33688164 None:intergenic
AGAGATCAAATGGCGTTGTA+TGG 0.444087 7.1:+33688283 MS.gene84220:CDS
AGGCTTTACGCATCTGGGAT+TGG 0.445060 7.1:+33688923 MS.gene84220:CDS
TGTAGAAAGTGTTTATGATC+GGG 0.446440 7.1:+33688640 MS.gene84220:CDS
GAGCTTCTCATTTCCTACTC+AGG 0.447631 7.1:+33688470 MS.gene84220:CDS
AGTCGGACATTATTTCTGAT+TGG 0.449239 7.1:+33688663 MS.gene84220:CDS
CGACTAGGCTTTACGCATCT+GGG 0.463113 7.1:+33688918 MS.gene84220:CDS
TCGAAGTTCGTCTGGAACAA+CGG 0.466627 7.1:-33688177 None:intergenic
GGATGATGAGTATGTGTTCA+AGG 0.475476 7.1:-33689362 None:intergenic
AGCTTCTCATTTCCTACTCA+GGG 0.477291 7.1:+33688471 MS.gene84220:CDS
GAAGCAAAATACCATGTCAT+TGG 0.477313 7.1:+33688112 MS.gene84220:CDS
GACTTACTTGGGAAAATGAT+AGG 0.480795 7.1:-33689545 None:intergenic
AAAACATTTAGAATGTGAAA+TGG 0.489252 7.1:-33688602 None:intergenic
TGCCAATGGAAGAGATCAAA+TGG 0.492988 7.1:+33688273 MS.gene84220:CDS
AAATAGTGGCAAAGTAATGC+AGG 0.495153 7.1:-33689406 None:intergenic
AGGCATGAGTAATCGAGATC+TGG 0.499675 7.1:+33689264 MS.gene84220:CDS
ATCAATGGTATTGCCGGAGA+AGG 0.501823 7.1:-33688530 None:intergenic
AAAACAATAAGCACAAAAGA+TGG 0.501903 7.1:+33688090 MS.gene84220:CDS
TTGTAGAAAGTGTTTATGAT+CGG 0.507043 7.1:+33688639 MS.gene84220:CDS
GGCAATACCATTGATCTACA+TGG 0.518929 7.1:+33688538 MS.gene84220:CDS
GCAAAGTGTAGGACTTACTT+GGG 0.535451 7.1:-33689556 None:intergenic
TGATTCAAAACTACCATCAC+GGG 0.536163 7.1:+33688499 MS.gene84220:CDS
ACATGTTCCATGTAGATCAA+TGG 0.539217 7.1:-33688545 None:intergenic
ACGGGACAAGTACCCTTCTC+CGG 0.541541 7.1:+33688517 MS.gene84220:CDS
TGAATCAAAGTCCCCTGAGT+AGG 0.555563 7.1:-33688483 None:intergenic
ATTGAAATTGATGTCGGAGG+AGG 0.558357 7.1:+33688863 MS.gene84220:CDS
TCAATGGTATTGCCGGAGAA+GGG 0.560453 7.1:-33688529 None:intergenic
GGTCATAGCTATAGAAAGAG+AGG 0.561468 7.1:-33688709 None:intergenic
ATTGGTTATTACAGAGACAA+TGG 0.561655 7.1:+33689054 MS.gene84220:CDS
CTATAATGATTTGCTTATGG+TGG 0.567060 7.1:+33688568 MS.gene84220:CDS
GGGAAAATGATAGGCGACAA+TGG 0.569669 7.1:-33689536 None:intergenic
ATTTGAATCGATGTGCGACT+AGG 0.574528 7.1:+33688903 MS.gene84220:CDS
GAAGGGTACTTGTCCCGTGA+TGG 0.581613 7.1:-33688512 None:intergenic
CAAAATACCATGTCATTGGT+AGG 0.581628 7.1:+33688116 MS.gene84220:CDS
TTGATTCAAAACTACCATCA+CGG 0.582434 7.1:+33688498 MS.gene84220:CDS
TCCCATGTATCGAGTACCGA+TGG 0.583531 7.1:-33688237 None:intergenic
AAGAAAAGAGCTAATGCCAA+TGG 0.586368 7.1:+33688259 MS.gene84220:CDS
TTCGAGAATAGCTTCCATGC+AGG 0.589158 7.1:-33688351 None:intergenic
GATTAATTGAAAGCCATGGA+AGG 0.599201 7.1:+33688065 None:intergenic
GCATCGATTGAAATTGATGT+CGG 0.606536 7.1:+33688857 MS.gene84220:CDS
GTTGATCAATTTGGGGACGA+AGG 0.608781 7.1:-33688730 None:intergenic
AATAGTGGCAAAGTAATGCA+GGG 0.611145 7.1:-33689405 None:intergenic
ACTCATTGTCAATGATGTCA+CGG 0.612953 7.1:-33689321 None:intergenic
ATCACTGAAAAGCAAAGCAG+AGG 0.615803 7.1:-33689179 None:intergenic
TGTTTCCGCTCGTATTGATG+CGG 0.625755 7.1:+33688790 MS.gene84220:CDS
TCCATCGGTACTCGATACAT+GGG 0.630624 7.1:+33688236 MS.gene84220:CDS
AGATTGAGTTGATCAATTTG+GGG 0.630889 7.1:-33688737 None:intergenic
AAGTGTTTATGATCGGGAGT+CGG 0.634120 7.1:+33688646 MS.gene84220:CDS
ATACCAAAAGTTGTCTCCAT+CGG 0.635175 7.1:+33688221 MS.gene84220:CDS
GTCATAGCTATAGAAAGAGA+GGG 0.642936 7.1:-33688708 None:intergenic
GAAACAATTTCAAGTAAGTG+AGG 0.645803 7.1:-33688773 None:intergenic
TCGATTGAAATTGATGTCGG+AGG 0.667501 7.1:+33688860 MS.gene84220:CDS
TACAGCACCTACCAATGACA+TGG 0.678009 7.1:-33688123 None:intergenic
ATAGTGGCAAAGTAATGCAG+GGG 0.698227 7.1:-33689404 None:intergenic
AGACAATGGAATCAAAATGG+CGG 0.698722 7.1:+33689068 MS.gene84220:CDS
GGAGAATGATGTTCAAATCG+TGG 0.698946 7.1:+33688811 MS.gene84220:CDS
GCTTCTCATTTCCTACTCAG+GGG 0.700039 7.1:+33688472 MS.gene84220:CDS
ATTCTCCGCATCAATACGAG+CGG 0.700760 7.1:-33688795 None:intergenic
AAGAGAGGGTTAGTTCTACG+AGG 0.701797 7.1:-33688694 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGATTTAAAATTCATCAAT+GGG + chr7.1:33689014-33689033 MS.gene84220:CDS 15.0%
!!! TTTGATTTAAAATTCATCAA+TGG + chr7.1:33689013-33689032 MS.gene84220:CDS 15.0%
!!! AAAACATTTAGAATGTGAAA+TGG - chr7.1:33688605-33688624 None:intergenic 20.0%
! AAAACAATAAGCACAAAAGA+TGG + chr7.1:33688090-33688109 MS.gene84220:CDS 25.0%
! GGTTTCTAATATAGCATAAT+AGG - chr7.1:33689386-33689405 None:intergenic 25.0%
! TAAAAATACTCTGTGAGAAA+TGG - chr7.1:33688952-33688971 None:intergenic 25.0%
! TCAAAACTTGATATTTCAAC+TGG - chr7.1:33688329-33688348 None:intergenic 25.0%
!!! ACAACCTTCACAAATTTTTT+AGG + chr7.1:33689452-33689471 MS.gene84220:CDS 25.0%
!!! CAGAGTATTTTTAAGACATT+AGG + chr7.1:33688959-33688978 MS.gene84220:CDS 25.0%
!!! TGTCTATAATGATTTGCTTA+TGG + chr7.1:33688565-33688584 MS.gene84220:CDS 25.0%
!!! TTGTAGAAAGTGTTTATGAT+CGG + chr7.1:33688639-33688658 MS.gene84220:CDS 25.0%
ATTGGTTATTACAGAGACAA+TGG + chr7.1:33689054-33689073 MS.gene84220:CDS 30.0%
TCAAATCGTGGAGAATAATA+AGG + chr7.1:33688823-33688842 MS.gene84220:CDS 30.0%
TTGATTCAAAACTACCATCA+CGG + chr7.1:33688498-33688517 MS.gene84220:CDS 30.0%
! ATTTCAGCAGGATTTTAGAT+TGG + chr7.1:33689425-33689444 MS.gene84220:CDS 30.0%
! GAAACAATTTCAAGTAAGTG+AGG - chr7.1:33688776-33688795 None:intergenic 30.0%
! GAAGAAGAGTTCTTCTTTTT+CGG + chr7.1:33689123-33689142 MS.gene84220:CDS 30.0%
!! AGATTGAGTTGATCAATTTG+GGG - chr7.1:33688740-33688759 None:intergenic 30.0%
!! CTATAATGATTTGCTTATGG+TGG + chr7.1:33688568-33688587 MS.gene84220:CDS 30.0%
!! CTTTTTCATTCAATCGCAAA+AGG + chr7.1:33689292-33689311 MS.gene84220:CDS 30.0%
!! GAGATTGAGTTGATCAATTT+GGG - chr7.1:33688741-33688760 None:intergenic 30.0%
!! TGAGATTGAGTTGATCAATT+TGG - chr7.1:33688742-33688761 None:intergenic 30.0%
!! TGTAGAAAGTGTTTATGATC+GGG + chr7.1:33688640-33688659 MS.gene84220:CDS 30.0%
AAATAGTGGCAAAGTAATGC+AGG - chr7.1:33689409-33689428 None:intergenic 35.0%
AAGAAAAGAGCTAATGCCAA+TGG + chr7.1:33688259-33688278 MS.gene84220:CDS 35.0%
AATAGTGGCAAAGTAATGCA+GGG - chr7.1:33689408-33689427 None:intergenic 35.0%
ACATGTTCCATGTAGATCAA+TGG - chr7.1:33688548-33688567 None:intergenic 35.0%
ACTCATTGTCAATGATGTCA+CGG - chr7.1:33689324-33689343 None:intergenic 35.0%
AGACAATGGAATCAAAATGG+CGG + chr7.1:33689068-33689087 MS.gene84220:CDS 35.0%
ATACCAAAAGTTGTCTCCAT+CGG + chr7.1:33688221-33688240 MS.gene84220:CDS 35.0%
ATGTAGATCAATGGTATTGC+CGG - chr7.1:33688539-33688558 None:intergenic 35.0%
CAAAATACCATGTCATTGGT+AGG + chr7.1:33688116-33688135 MS.gene84220:CDS 35.0%
CAACTCAATCTCAACTTCAA+TGG + chr7.1:33688749-33688768 MS.gene84220:CDS 35.0%
CACGCCTAAAAAATTTGTGA+AGG - chr7.1:33689459-33689478 None:intergenic 35.0%
CAGAGACAATGGAATCAAAA+TGG + chr7.1:33689065-33689084 MS.gene84220:CDS 35.0%
CGAGTTATACGTAACGATTT+AGG + chr7.1:33689244-33689263 MS.gene84220:CDS 35.0%
CTAAAATCCTGCTGAAATAG+TGG - chr7.1:33689423-33689442 None:intergenic 35.0%
GAAGCAAAATACCATGTCAT+TGG + chr7.1:33688112-33688131 MS.gene84220:CDS 35.0%
GTATGATCTTGCTACAGTTA+TGG + chr7.1:33688424-33688443 MS.gene84220:CDS 35.0%
GTCATAGCTATAGAAAGAGA+GGG - chr7.1:33688711-33688730 None:intergenic 35.0%
GTCTCTGTAATAACCAATTC+TGG - chr7.1:33689052-33689071 None:intergenic 35.0%
TATTGTTCGTGCAAGCTATA+TGG + chr7.1:33688382-33688401 MS.gene84220:CDS 35.0%
TGATTCAAAACTACCATCAC+GGG + chr7.1:33688499-33688518 MS.gene84220:CDS 35.0%
! GCTACAGTTATGGTTTTTGA+TGG + chr7.1:33688434-33688453 MS.gene84220:CDS 35.0%
! TTCAATTTTCGAAGTTCGTC+TGG - chr7.1:33688188-33688207 None:intergenic 35.0%
!! AGTCGGACATTATTTCTGAT+TGG + chr7.1:33688663-33688682 MS.gene84220:CDS 35.0%
!! GCATCGATTGAAATTGATGT+CGG + chr7.1:33688857-33688876 MS.gene84220:CDS 35.0%
!!! CTTCACAAATTTTTTAGGCG+TGG + chr7.1:33689457-33689476 MS.gene84220:CDS 35.0%
AAGTGTTTATGATCGGGAGT+CGG + chr7.1:33688646-33688665 MS.gene84220:CDS 40.0%
AGAGATCAAATGGCGTTGTA+TGG + chr7.1:33688283-33688302 MS.gene84220:CDS 40.0%
AGCTTCTCATTTCCTACTCA+GGG + chr7.1:33688471-33688490 MS.gene84220:CDS 40.0%
ATAGTGGCAAAGTAATGCAG+GGG - chr7.1:33689407-33689426 None:intergenic 40.0%
ATCACTGAAAAGCAAAGCAG+AGG - chr7.1:33689182-33689201 None:intergenic 40.0%
ATTGAAATTGATGTCGGAGG+AGG + chr7.1:33688863-33688882 MS.gene84220:CDS 40.0%
ATTTGAATCGATGTGCGACT+AGG + chr7.1:33688903-33688922 MS.gene84220:CDS 40.0%
GACACTACTTGTTCCAGAAT+TGG + chr7.1:33689036-33689055 MS.gene84220:CDS 40.0%
GGAGAATGATGTTCAAATCG+TGG + chr7.1:33688811-33688830 MS.gene84220:CDS 40.0%
GGATGATGAGTATGTGTTCA+AGG - chr7.1:33689365-33689384 None:intergenic 40.0%
GGCAATACCATTGATCTACA+TGG + chr7.1:33688538-33688557 MS.gene84220:CDS 40.0%
GGTCATAGCTATAGAAAGAG+AGG - chr7.1:33688712-33688731 None:intergenic 40.0%
TACTTTGCCACTATTTCAGC+AGG + chr7.1:33689413-33689432 MS.gene84220:CDS 40.0%
TCGATTGAAATTGATGTCGG+AGG + chr7.1:33688860-33688879 MS.gene84220:CDS 40.0%
TGCCAATGGAAGAGATCAAA+TGG + chr7.1:33688273-33688292 MS.gene84220:CDS 40.0%
! AATGGCGGAATTTCATTGCT+TGG + chr7.1:33689083-33689102 MS.gene84220:CDS 40.0%
! ATGGCGGAATTTCATTGCTT+GGG + chr7.1:33689084-33689103 MS.gene84220:CDS 40.0%
!! ATCAAGTTTTGACACCTGCA+TGG + chr7.1:33688337-33688356 MS.gene84220:CDS 40.0%
!! TGCTACGTTGATAACTCTTC+TGG + chr7.1:33689492-33689511 MS.gene84220:CDS 40.0%
AGGCATGAGTAATCGAGATC+TGG + chr7.1:33689264-33689283 MS.gene84220:CDS 45.0%
ATCAATGGTATTGCCGGAGA+AGG - chr7.1:33688533-33688552 None:intergenic 45.0%
ATTCTCCGCATCAATACGAG+CGG - chr7.1:33688798-33688817 None:intergenic 45.0%
CGCCATTTGATCTCTTCCAT+TGG - chr7.1:33688278-33688297 None:intergenic 45.0%
GAGCTTCTCATTTCCTACTC+AGG + chr7.1:33688470-33688489 MS.gene84220:CDS 45.0%
GCTTCTCATTTCCTACTCAG+GGG + chr7.1:33688472-33688491 MS.gene84220:CDS 45.0%
GGAACAACGGGAATGCTATA+TGG - chr7.1:33688167-33688186 None:intergenic 45.0%
GGGAAAATGATAGGCGACAA+TGG - chr7.1:33689539-33689558 None:intergenic 45.0%
GTTGATCAATTTGGGGACGA+AGG - chr7.1:33688733-33688752 None:intergenic 45.0%
TACAGCACCTACCAATGACA+TGG - chr7.1:33688126-33688145 None:intergenic 45.0%
TCAATGGTATTGCCGGAGAA+GGG - chr7.1:33688532-33688551 None:intergenic 45.0%
TCCATCGGTACTCGATACAT+GGG + chr7.1:33688236-33688255 MS.gene84220:CDS 45.0%
TGAATCAAAGTCCCCTGAGT+AGG - chr7.1:33688486-33688505 None:intergenic 45.0%
TTCGAGAATAGCTTCCATGC+AGG - chr7.1:33688354-33688373 None:intergenic 45.0%
! TCGAAGTTCGTCTGGAACAA+CGG - chr7.1:33688180-33688199 None:intergenic 45.0%
! TGTTTCCGCTCGTATTGATG+CGG + chr7.1:33688790-33688809 MS.gene84220:CDS 45.0%
!! AAGAGAGGGTTAGTTCTACG+AGG - chr7.1:33688697-33688716 None:intergenic 45.0%
!! CTTGGGAGCATCACAAGAAA+TGG + chr7.1:33689101-33689120 MS.gene84220:CDS 45.0%
!! GTACCGATGGAGACAACTTT+TGG - chr7.1:33688227-33688246 None:intergenic 45.0%
CGACTAGGCTTTACGCATCT+GGG + chr7.1:33688918-33688937 MS.gene84220:CDS 50.0%
CTCCATCGGTACTCGATACA+TGG + chr7.1:33688235-33688254 MS.gene84220:CDS 50.0%
! AGGCTTTACGCATCTGGGAT+TGG + chr7.1:33688923-33688942 MS.gene84220:CDS 50.0%
! CGAAGTTCGTCTGGAACAAC+GGG - chr7.1:33688179-33688198 None:intergenic 50.0%
!! TCCCATGTATCGAGTACCGA+TGG - chr7.1:33688240-33688259 None:intergenic 50.0%
ACGGGACAAGTACCCTTCTC+CGG + chr7.1:33688517-33688536 MS.gene84220:CDS 55.0%
GCGACTAGGCTTTACGCATC+TGG + chr7.1:33688917-33688936 MS.gene84220:CDS 55.0%
!! GAAGGGTACTTGTCCCGTGA+TGG - chr7.1:33688515-33688534 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 33688080 33689564 33688080 ID=MS.gene84220
chr7.1 mRNA 33688080 33689564 33688080 ID=MS.gene84220.t1;Parent=MS.gene84220
chr7.1 exon 33688080 33689564 33688080 ID=MS.gene84220.t1.exon1;Parent=MS.gene84220.t1
chr7.1 CDS 33688080 33689564 33688080 ID=cds.MS.gene84220.t1;Parent=MS.gene84220.t1
Gene Sequence

>MS.gene84220

ATGGAAGGAGAAAACAATAAGCACAAAAGATGGAAGCAAAATACCATGTCATTGGTAGGTGCTGTATCTCATGATTTTCTTCAACCATATAGCATTCCCGTTGTTCCAGACGAACTTCGAAAATTGAATGAGAATGCTTACATACCAAAAGTTGTCTCCATCGGTACTCGATACATGGGAAGAAAAGAGCTAATGCCAATGGAAGAGATCAAATGGCGTTGTATGGTTTATCTTCTTTCTCGATCACCAGTTGAAATATCAAGTTTTGACACCTGCATGGAAGCTATTCTCGAATTAGATTCTATTGTTCGTGCAAGCTATATGGACAAAGTCAAACTAGACGAGTATGATCTTGCTACAGTTATGGTTTTTGATGGTTGTTTTTTGTTAGAGCTTCTCATTTCCTACTCAGGGGACTTTGATTCAAAACTACCATCACGGGACAAGTACCCTTCTCCGGCAATACCATTGATCTACATGGAACATGTCTATAATGATTTGCTTATGGTGGAGAATCAAATACCATTTCACATTCTAAATGTTTTGTTTCGCAAACTTTTTGTAGAAAGTGTTTATGATCGGGAGTCGGACATTATTTCTGATTGGAGTTCAAACCTCGTAGAACTAACCCTCTCTTTCTATAGCTATGACCTTCGTCCCCAAATTGATCAACTCAATCTCAACTTCAATGGACCTCACTTACTTGAAATTGTTTCCGCTCGTATTGATGCGGAGAATGATGTTCAAATCGTGGAGAATAATAAGGAAATAAAGCATGCATCGATTGAAATTGATGTCGGAGGAGGTACAAGTTCAATGAAACATTTGAATCGATGTGCGACTAGGCTTTACGCATCTGGGATTGGTTTCCATTTCTCACAGAGTATTTTTAAGACATTAGGTTATTCTGCTCATGTCAAAAACATGTTCAGTTTTGATTTAAAATTCATCAATGGGACACTACTTGTTCCAGAATTGGTTATTACAGAGACAATGGAATCAAAATGGCGGAATTTCATTGCTTGGGAGCATCACAAGAAATGGAAGAAGAGTTCTTCTTTTTCGGCTGATTCTGAAAAGTGTTGCAGCTTATTCACTTCCTCTGCTTTGCTTTTCAGTGATTTAATATGCTGTACAGCTGATGTTCAATTGCTCATAGATCAACGAGTTATACGTAACGATTTAGGCATGAGTAATCGAGATCTGGCTGCTCTTTTTCATTCAATCGCAAAAGGAGTTGACCGTGACATCATTGACAATGAGTATAGCAAGATGTTTCATGCCTTGAACACATACTCATCATCCTATTATGCTATATTAGAAACCCCTGCATTACTTTGCCACTATTTCAGCAGGATTTTAGATTGGATTTACAACCTTCACAAATTTTTTAGGCGTGGTTACAACTTTGCTGCTACGTTGATAACTCTTCTGGCTGTTGTTCAGACTTGTTATGCCATTGTCGCCTATCATTTTCCCAAGTAA
Protein sequence

>MS.gene84220.t1

MEGENNKHKRWKQNTMSLVGAVSHDFLQPYSIPVVPDELRKLNENAYIPKVVSIGTRYMGRKELMPMEEIKWRCMVYLLSRSPVEISSFDTCMEAILELDSIVRASYMDKVKLDEYDLATVMVFDGCFLLELLISYSGDFDSKLPSRDKYPSPAIPLIYMEHVYNDLLMVENQIPFHILNVLFRKLFVESVYDRESDIISDWSSNLVELTLSFYSYDLRPQIDQLNLNFNGPHLLEIVSARIDAENDVQIVENNKEIKHASIEIDVGGGTSSMKHLNRCATRLYASGIGFHFSQSIFKTLGYSAHVKNMFSFDLKFINGTLLVPELVITETMESKWRNFIAWEHHKKWKKSSSFSADSEKCCSLFTSSALLFSDLICCTADVQLLIDQRVIRNDLGMSNRDLAALFHSIAKGVDRDIIDNEYSKMFHALNTYSSSYYAILETPALLCHYFSRILDWIYNLHKFFRRGYNFAATLITLLAVVQTCYAIVAYHFPK