AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene84662

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84662.t1 MTR_8g020970 98.921 927 10 0 1 927 1 927 0.0 1905
MS.gene84662.t1 MTR_8g020970 98.817 676 8 0 1 676 1 676 0.0 1384
MS.gene84662.t1 MTR_8g080230 54.157 842 378 6 89 926 78 915 0.0 942
MS.gene84662.t1 MTR_4g088810 54.448 843 350 10 90 927 82 895 0.0 920
MS.gene84662.t1 MTR_4g066170 48.446 869 431 11 64 926 48 905 0.0 841
MS.gene84662.t1 MTR_3g479460 48.089 811 404 8 122 926 103 902 0.0 807
MS.gene84662.t1 MTR_3g079450 48.089 811 404 10 122 926 102 901 0.0 801
MS.gene84662.t1 MTR_3g079420 45.853 844 439 10 90 926 32 864 0.0 763
MS.gene84662.t1 MTR_1g083020 45.731 855 453 9 79 926 7 857 0.0 754
MS.gene84662.t1 MTR_8g018420 47.002 834 425 11 100 926 32 855 0.0 751
MS.gene84662.t1 MTR_8g018730 47.445 822 411 11 113 926 55 863 0.0 748
MS.gene84662.t1 MTR_7g113410 46.635 847 437 13 91 926 24 866 0.0 747
MS.gene84662.t1 MTR_8g018735 47.259 821 412 12 114 926 57 864 0.0 746
MS.gene84662.t1 MTR_5g024020 46.780 823 416 13 113 926 57 866 0.0 740
MS.gene84662.t1 MTR_2g099560 47.023 823 412 15 113 926 55 862 0.0 738
MS.gene84662.t1 MTR_2g099570 46.036 845 434 14 91 926 29 860 0.0 735
MS.gene84662.t1 MTR_8g018730 47.253 819 387 12 113 926 55 833 0.0 729
MS.gene84662.t1 MTR_8g018570 46.594 822 420 13 114 926 58 869 0.0 726
MS.gene84662.t1 MTR_1g104650 44.691 857 457 12 84 926 2 855 0.0 723
MS.gene84662.t1 MTR_8g018520 45.676 821 429 10 113 926 57 867 0.0 723
MS.gene84662.t1 MTR_8g020990 45.432 821 431 11 113 926 57 867 0.0 717
MS.gene84662.t1 MTR_8g018690 45.477 818 430 9 113 926 59 864 0.0 715
MS.gene84662.t1 MTR_8g018510 45.455 814 427 11 120 926 65 868 0.0 712
MS.gene84662.t1 MTR_3g081130 46.489 826 413 12 113 926 49 857 0.0 707
MS.gene84662.t1 MTR_8g018430 44.580 821 438 11 113 926 50 860 0.0 706
MS.gene84662.t1 MTR_8g018550 46.163 821 425 11 113 926 57 867 0.0 704
MS.gene84662.t1 MTR_3g081130 47.809 776 387 10 157 926 22 785 0.0 701
MS.gene84662.t1 MTR_5g024020 44.836 823 396 15 113 926 57 830 0.0 689
MS.gene84662.t1 MTR_8g018690 44.377 818 419 10 113 926 59 844 0.0 686
MS.gene84662.t1 MTR_8g018450 43.688 808 408 10 128 926 58 827 0.0 674
MS.gene84662.t1 MTR_8g018590 44.052 765 380 12 177 926 91 822 0.0 635
MS.gene84662.t1 MTR_8g018650 43.286 767 381 9 164 926 90 806 0.0 614
MS.gene84662.t1 MTR_8g018620 43.229 768 380 11 164 926 90 806 0.0 607
MS.gene84662.t1 MTR_8g011340 39.371 795 460 10 102 882 36 822 0.0 575
MS.gene84662.t1 MTR_8g018730 48.557 589 288 8 344 926 4 583 0.0 560
MS.gene84662.t1 MTR_3g079450 46.774 558 285 7 122 676 102 650 1.27e-176 527
MS.gene84662.t1 MTR_8g018735 45.709 571 290 11 114 676 57 615 1.42e-156 475
MS.gene84662.t1 MTR_6g452500 37.908 612 358 9 313 913 11 611 1.10e-133 415
MS.gene84662.t1 MTR_8g021020 46.835 395 207 3 534 926 37 430 1.34e-122 379
MS.gene84662.t1 MTR_7g012030 58.824 119 49 0 578 696 85 203 1.07e-43 157
MS.gene84662.t1 MTR_8g463810 36.000 225 80 2 488 712 197 357 1.21e-39 152
MS.gene84662.t1 MTR_6g046330 53.077 130 50 1 578 696 75 204 4.06e-38 145
MS.gene84662.t1 MTR_4g088805 58.696 92 38 0 836 927 1 92 1.17e-28 110
MS.gene84662.t1 MTR_0248s0010 67.442 43 2 1 732 774 3 33 5.97e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84662.t1 AT1G17420 70.119 927 267 5 1 926 1 918 0.0 1376
MS.gene84662.t1 AT1G72520 70.909 935 253 10 1 926 1 925 0.0 1360
MS.gene84662.t1 AT1G67560 51.241 927 424 13 7 926 11 916 0.0 904
MS.gene84662.t1 AT3G45140 44.355 868 453 14 66 926 51 895 0.0 754
MS.gene84662.t1 AT1G55020 45.626 846 444 13 90 926 20 858 0.0 743
MS.gene84662.t1 AT3G22400 44.780 862 455 11 83 926 27 885 0.0 735
MS.gene84662.t1 AT3G45140 47.368 760 384 10 173 926 4 753 0.0 726

Find 186 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTGAAAATGTGTTCATTT+TGG 0.118061 8.2:-74110230 None:intergenic
TTTACGGTTGACTCAAATTT+TGG 0.128121 8.2:+74107314 MS.gene84662:CDS
GCAAGATCAATTCCTTTATC+TGG 0.137436 8.2:-74108641 None:intergenic
CAACATGCTGCACTTAATTT+TGG 0.137566 8.2:+74114396 MS.gene84662:CDS
CTTTAGATGCAACTACTAAT+TGG 0.156046 8.2:+74112578 MS.gene84662:CDS
GAGCAGAAAGACTTGATTTA+AGG 0.159246 8.2:-74110291 None:intergenic
ACTCTCTGTCATTGATTATT+AGG 0.161431 8.2:-74114242 None:intergenic
TTTGGTCAGCAATAGAAAAT+TGG 0.164309 8.2:+74114181 MS.gene84662:CDS
TATCAATTCTCTGCTGAAAT+TGG 0.176100 8.2:+74114681 MS.gene84662:CDS
TTGTCACTGTCGTTCAATTT+TGG 0.218955 8.2:-74114330 None:intergenic
CAGAAGATGGTCTTCTAATT+TGG 0.251107 8.2:+74114163 MS.gene84662:CDS
TCTTGTGGACCATAGATTTC+AGG 0.265813 8.2:-74111674 None:intergenic
GAATTACTTGCTCCTAGTTC+TGG 0.266117 8.2:+74114783 MS.gene84662:CDS
AAGTCCTAACAATCTTAATT+TGG 0.267658 8.2:+74114361 MS.gene84662:CDS
CATTCCAAATTAAGATTGTT+AGG 0.277408 8.2:-74114365 None:intergenic
TACCCTGGCTTGTGGATTCT+TGG 0.281500 8.2:-74110438 None:intergenic
TCGTCTGACGAAGAGTATTT+AGG 0.285117 8.2:+74114603 MS.gene84662:CDS
GAAAAGAAAATTCTCTTTCC+AGG 0.286608 8.2:-74107470 None:intergenic
GATATGAAACTTGTATATTC+AGG 0.290338 8.2:-74114492 None:intergenic
TTGATTATTAGGCTTGGATT+TGG 0.309327 8.2:-74114231 None:intergenic
TCCTCAAATCTTTCATCTCT+TGG 0.311154 8.2:-74110205 None:intergenic
TCAGGATCTAATTTACTAAC+AGG 0.316657 8.2:-74111656 None:intergenic
GTTGCAAGCTACAAAATATA+TGG 0.317917 8.2:+74114554 MS.gene84662:CDS
TAATTACACAGCTGAATTTA+CGG 0.322203 8.2:+74107298 MS.gene84662:CDS
CCACTCACTTGATATAATCT+TGG 0.323901 8.2:-74110479 None:intergenic
AATGAATTCTCAGATGTTGA+TGG 0.326863 8.2:+74110328 MS.gene84662:CDS
GTCAAAGAAGACTAATATTA+AGG 0.327000 8.2:+74107265 MS.gene84662:CDS
ATCCTTCAACACTGCTTCAT+TGG 0.329544 8.2:-74107240 None:intergenic
GTATTAATCTTCTCATCAAC+GGG 0.342100 8.2:-74114455 None:intergenic
CATTTATATTGTCTGCTCAT+AGG 0.356521 8.2:+74113079 MS.gene84662:CDS
CTGTCATTGATTATTAGGCT+TGG 0.359024 8.2:-74114237 None:intergenic
GGATCCAATAACTTGAAAAT+TGG 0.359274 8.2:-74113120 None:intergenic
CTATGAGCAGACAATATAAA+TGG 0.371741 8.2:-74113078 None:intergenic
GTAAGTCTCTCAATGGTAAC+AGG 0.380176 8.2:-74111623 None:intergenic
AGAGGCAACAGCCTTCGATT+TGG 0.381268 8.2:+74114628 MS.gene84662:CDS
TTAGAAAATATAACAATTGA+AGG 0.381425 8.2:+74107386 MS.gene84662:CDS
GTGCTTCATAACTTGCTTCC+TGG 0.384287 8.2:+74110268 MS.gene84662:CDS
GATCTTGGAAACCCAGATAA+AGG 0.388829 8.2:+74108629 MS.gene84662:CDS
GGCCCTAACAAAAGAATTCA+TGG 0.391351 8.2:+74106679 MS.gene84662:CDS
GGTGAAGAAGAAGGAAATAT+TGG 0.394404 8.2:-74106722 None:intergenic
AGAATTCATGGGTTCTTCAT+TGG 0.395604 8.2:+74106691 MS.gene84662:CDS
TTGAGGAACCGTTGCGGTGC+TGG 0.401483 8.2:+74114747 MS.gene84662:CDS
TTGGTCAGCAATAGAAAATT+GGG 0.402009 8.2:+74114182 MS.gene84662:CDS
GAACTTAGCCTGCCTCCATC+TGG 0.403989 8.2:+74112520 MS.gene84662:CDS
TATGACACATACAATGATCT+TGG 0.412730 8.2:+74108614 MS.gene84662:CDS
CACTCACTTGATATAATCTT+GGG 0.413543 8.2:-74110478 None:intergenic
AGAAGACCATCTTCTGCATA+AGG 0.416449 8.2:-74114156 None:intergenic
TCTTGGGAATGTCATACTTA+AGG 0.423843 8.2:-74110462 None:intergenic
AAATTAAGATTGTTAGGACT+TGG 0.428810 8.2:-74114359 None:intergenic
TTTAGATGCAACTACTAATT+GGG 0.429267 8.2:+74112579 MS.gene84662:CDS
TGAGTAGTCCAACACTATAA+AGG 0.430450 8.2:-74110351 None:intergenic
TTAGACCATGTGGTTGTGTT+GGG 0.432526 8.2:-74114113 None:intergenic
GATCTTGAGGGAGGCTATCC+TGG 0.433367 8.2:-74113287 None:intergenic
TGATTATTAGGCTTGGATTT+GGG 0.433593 8.2:-74114230 None:intergenic
TGTTGGACTACTCATTAAGT+TGG 0.436827 8.2:+74110360 MS.gene84662:CDS
AGGCTAAGTTCAATTGCAAC+AGG 0.441630 8.2:-74112508 None:intergenic
TCTTTAAGTGCAGACTCTTG+TGG 0.444467 8.2:-74111689 None:intergenic
CAAGATCAATTCCTTTATCT+GGG 0.447942 8.2:-74108640 None:intergenic
ATTGAGTCTTGCTTCACTCC+TGG 0.448673 8.2:+74113210 MS.gene84662:CDS
CCATGATTCGTCTTTCATAT+CGG 0.451611 8.2:-74114304 None:intergenic
GACCAATGAAGCAGTGTTGA+AGG 0.454038 8.2:+74107238 MS.gene84662:CDS
ATGGAAACAAAAGAGTTGTA+AGG 0.456748 8.2:+74106798 MS.gene84662:CDS
TTTGTAGCTTGCAACAAACT+AGG 0.458682 8.2:-74114546 None:intergenic
TTGGTGCACACCAGCATCAT+TGG 0.460866 8.2:-74112632 None:intergenic
CAAGCTAGGAAAGATCTTCC+TGG 0.462757 8.2:+74107452 MS.gene84662:CDS
TCGAGAAGGTGATTGATAGT+AGG 0.464448 8.2:+74114709 MS.gene84662:CDS
GAAAGACTTGATTTAAGGCC+AGG 0.464489 8.2:-74110286 None:intergenic
ACAATTGAAGGATTTGCTAC+TGG 0.466343 8.2:+74107398 MS.gene84662:CDS
AAATTAGATCCTGAAATCTA+TGG 0.468171 8.2:+74111665 MS.gene84662:CDS
GTGGCCTACATTGATTGATT+CGG 0.469250 8.2:-74114280 None:intergenic
AACTCCAGCTATTGCTTGAC+GGG 0.470233 8.2:-74111597 None:intergenic
TTTGGCACGTACCCTCCATA+AGG 0.471877 8.2:-74114426 None:intergenic
TCTTCACTGATAGCTGCAAC+AGG 0.474972 8.2:-74106842 None:intergenic
GTTGGACTACTCATTAAGTT+GGG 0.477312 8.2:+74110361 MS.gene84662:CDS
ACCAAGAGATGAAAGATTTG+AGG 0.477798 8.2:+74110204 MS.gene84662:CDS
TTTAGACCATGTGGTTGTGT+TGG 0.478176 8.2:-74114114 None:intergenic
GCAATTCTTGGGTTCAAGCT+AGG 0.478592 8.2:+74107438 MS.gene84662:CDS
TTTGGAAAGGATCAATGCCT+TGG 0.478720 8.2:+74112423 MS.gene84662:CDS
GATCTTGCTAGACCAACTCT+TGG 0.479530 8.2:+74108656 MS.gene84662:CDS
GCTCTAACCTTAAATTTCAC+AGG 0.486545 8.2:-74106932 None:intergenic
CCATGTGGTTGTGTTGGGTC+TGG 0.492360 8.2:-74114108 None:intergenic
TCATCTCTTGGTATATACAT+AGG 0.492403 8.2:-74110193 None:intergenic
TAACTCCAGCTATTGCTTGA+CGG 0.493184 8.2:-74111598 None:intergenic
CGGCCAGTACGGCACCGTCT+AGG 0.495310 8.2:-74108701 None:intergenic
ATGAAGCAGTGTTGAAGGAT+TGG 0.497542 8.2:+74107243 MS.gene84662:CDS
TACCCTAGACGGTGCCGTAC+TGG 0.498027 8.2:+74108698 MS.gene84662:CDS
GAAGACCATCTTCTGCATAA+GGG 0.501628 8.2:-74114155 None:intergenic
TGCACCAACTTGCCCACCAT+TGG 0.505334 8.2:+74112647 MS.gene84662:CDS
CTAGGAGCAAGTAATTCATA+AGG 0.506030 8.2:-74114777 None:intergenic
CTCAAAAGTTTAATCCCAGC+AGG 0.506743 8.2:-74108521 None:intergenic
CATGTTTGCTCCAATGATGC+TGG 0.508665 8.2:+74112622 MS.gene84662:CDS
GGCCGTGAACCATCTGATAC+AGG 0.510574 8.2:+74108719 MS.gene84662:CDS
TTTATCACAGTAATAGCTCC+TGG 0.510933 8.2:-74107341 None:intergenic
TACCTGTATCAGATGGTTCA+CGG 0.511896 8.2:-74108721 None:intergenic
CTGCTGCTTACAAAACCAAC+TGG 0.511913 8.2:+74113256 MS.gene84662:CDS
GATCCAAGAATCCACAAGCC+AGG 0.513956 8.2:+74110435 MS.gene84662:CDS
CTTGCTCCTAGTTCTGGACC+TGG 0.515196 8.2:+74114789 MS.gene84662:CDS
GTACGGCACCGTCTAGGGTA+TGG 0.515752 8.2:-74108695 None:intergenic
CTACCCGATGATACACCTGC+TGG 0.515885 8.2:+74108506 MS.gene84662:CDS
GGTGTATCATCGGGTAGATA+AGG 0.517991 8.2:-74108500 None:intergenic
TGAAAACATGGTGAAGAAGA+AGG 0.520459 8.2:-74106731 None:intergenic
GTTGATGGCCTTTATAGTGT+TGG 0.523396 8.2:+74110343 MS.gene84662:CDS
CTTAAGGATACCCTGGCTTG+TGG 0.525590 8.2:-74110446 None:intergenic
ATCTCCATGGCATAGCGACC+AGG 0.526081 8.2:-74113228 None:intergenic
GACCTGGTGTTACATGCAGA+GGG 0.527475 8.2:+74114805 MS.gene84662:CDS
GGTATTAATCTTCTCATCAA+CGG 0.530886 8.2:-74114456 None:intergenic
ATATGAAACTTGTATATTCA+GGG 0.530940 8.2:-74114491 None:intergenic
TTTGCCCGTCAAGCAATAGC+TGG 0.532353 8.2:+74111593 MS.gene84662:CDS
CTTACCAATGGTGGGCAAGT+TGG 0.533372 8.2:-74112651 None:intergenic
ATCCCAGCAGGTGTATCATC+GGG 0.533446 8.2:-74108509 None:intergenic
CACTCCTGGTCGCTATGCCA+TGG 0.533642 8.2:+74113224 MS.gene84662:CDS
TGTTTGGTCCAGATGGAGGC+AGG 0.533655 8.2:-74112528 None:intergenic
ATTTCAGATCCTCCAAGAGT+TGG 0.535524 8.2:-74108668 None:intergenic
CCGATATGAAAGACGAATCA+TGG 0.537046 8.2:+74114304 MS.gene84662:CDS
CTTGCTAGACCAACTCTTGG+AGG 0.537115 8.2:+74108659 MS.gene84662:CDS
CAACAGCCTTCGATTTGGAC+GGG 0.537627 8.2:+74114633 MS.gene84662:CDS
TAATTATTACCTGTATCAGA+TGG 0.538174 8.2:-74108728 None:intergenic
AAGGTGATTGATAGTAGGAA+TGG 0.538476 8.2:+74114714 MS.gene84662:CDS
TTGGCACGTACCCTCCATAA+GGG 0.539174 8.2:-74114425 None:intergenic
GATTACCCTTATGCAGAAGA+TGG 0.539224 8.2:+74114150 MS.gene84662:CDS
GAAAGGATCAATGCCTTGGA+TGG 0.543205 8.2:+74112427 MS.gene84662:CDS
GTAACACCAGGTCCAGAACT+AGG 0.543782 8.2:-74114795 None:intergenic
TTGCTAACAATGTGAGGCAA+TGG 0.546196 8.2:-74110412 None:intergenic
TTGATAGTAGGAATGGTGAT+AGG 0.548139 8.2:+74114721 MS.gene84662:CDS
CCATTCTTGTGTTGAAAACA+TGG 0.549764 8.2:-74106743 None:intergenic
CTTGGTATATACATAGGAAG+TGG 0.550676 8.2:-74110187 None:intergenic
TCCACACATGAGGTACACTT+TGG 0.551392 8.2:+74113140 MS.gene84662:CDS
GGAATGGTGATAGGAATTTG+AGG 0.553948 8.2:+74114730 MS.gene84662:CDS
GGACCTGGTGTTACATGCAG+AGG 0.558911 8.2:+74114804 MS.gene84662:CDS
AATCCCAGCAGGTGTATCAT+CGG 0.559593 8.2:-74108510 None:intergenic
ACCTGCGGATGAGATCTTGA+GGG 0.562889 8.2:-74113299 None:intergenic
GGCCAGTACGGCACCGTCTA+GGG 0.564885 8.2:-74108700 None:intergenic
GCAACAGCCTTCGATTTGGA+CGG 0.566610 8.2:+74114632 MS.gene84662:CDS
GATCGAGTGTTTGGTCCAGA+TGG 0.566962 8.2:-74112535 None:intergenic
TTAGTAGTTGCATCTAAAGC+AGG 0.568173 8.2:-74112574 None:intergenic
GTTGATGAGAAGATTAATAC+CGG 0.571822 8.2:+74114458 MS.gene84662:CDS
TCCCTCAAGATCTCATCCGC+AGG 0.574537 8.2:+74113298 MS.gene84662:CDS
GGATCTAATTTACTAACAGG+TGG 0.574715 8.2:-74111653 None:intergenic
GTGCAGAGGATAAATTTGCT+TGG 0.574838 8.2:+74111555 MS.gene84662:intron
GATCTAATTTACTAACAGGT+GGG 0.574850 8.2:-74111652 None:intergenic
GCTACGCACACATGCTTGCA+TGG 0.575061 8.2:+74113053 MS.gene84662:CDS
AATCTGAGAGGTGATGGAAA+AGG 0.575229 8.2:+74108560 MS.gene84662:CDS
GAAAACTGTAAGTCTCTCAA+TGG 0.578316 8.2:-74111630 None:intergenic
ATCCTGGTCAAAACGCCAGT+TGG 0.579614 8.2:-74113271 None:intergenic
AAATGTATCCATACCCTAGA+CGG 0.586694 8.2:+74108687 MS.gene84662:CDS
ATCCAAGAATCCACAAGCCA+GGG 0.589581 8.2:+74110436 MS.gene84662:CDS
ATTATTAGCTTTAGACCATG+TGG 0.590586 8.2:-74114123 None:intergenic
TACCCGATGATACACCTGCT+GGG 0.591254 8.2:+74108507 MS.gene84662:CDS
TTTGGACAGTACCCTTATGG+AGG 0.594124 8.2:+74114414 MS.gene84662:CDS
TACCTGCGGATGAGATCTTG+AGG 0.594715 8.2:-74113300 None:intergenic
CAGATGGTTCACGGCCAGTA+CGG 0.595137 8.2:-74108712 None:intergenic
AGCAGCAGAACTAATCTCCA+TGG 0.597060 8.2:-74113241 None:intergenic
TTGGACAGTACCCTTATGGA+GGG 0.597254 8.2:+74114415 MS.gene84662:CDS
TGGATCTTGCTAACAATGTG+AGG 0.598996 8.2:-74110418 None:intergenic
GTATGCTTGCTTACCAATGG+TGG 0.599350 8.2:-74112660 None:intergenic
GCATCACCCGTCCAAATCGA+AGG 0.600712 8.2:-74114639 None:intergenic
CAGAGCTTGATCAATGCAGA+TGG 0.602184 8.2:+74113183 MS.gene84662:CDS
TGTCATACTTAAGGATACCC+TGG 0.608790 8.2:-74110453 None:intergenic
TCCAAAGTGTACCTCATGTG+TGG 0.612282 8.2:-74113141 None:intergenic
CGAGTGTTTGGTCCAGATGG+AGG 0.614080 8.2:-74112532 None:intergenic
GCCCTAACAAAAGAATTCAT+GGG 0.614296 8.2:+74106680 MS.gene84662:CDS
TTTAAATGGTACCTCTTGGA+CGG 0.616838 8.2:-74111744 None:intergenic
ACCCCTCTGCATGTAACACC+AGG 0.617716 8.2:-74114807 None:intergenic
ATGCAACTACTAATTGGGTG+TGG 0.623131 8.2:+74112584 MS.gene84662:CDS
ACGAAGAGTATTTAGGTGAG+AGG 0.628094 8.2:+74114610 MS.gene84662:CDS
ATGACAGAGAGTTACAAGCT+TGG 0.632533 8.2:+74114253 MS.gene84662:CDS
TACTCCGAATCAATCAATGT+AGG 0.641645 8.2:+74114276 MS.gene84662:CDS
GCAAAACACCAGCACCGCAA+CGG 0.642856 8.2:-74114755 None:intergenic
GCTTGAACCCAAGAATTGCA+AGG 0.647001 8.2:-74107434 None:intergenic
AGGAATTTGAGGAACCGTTG+CGG 0.653263 8.2:+74114741 MS.gene84662:CDS
TGAAATTGGAAAGATCGAGA+AGG 0.656069 8.2:+74114695 MS.gene84662:CDS
TATGCTTGCTTACCAATGGT+GGG 0.656904 8.2:-74112659 None:intergenic
TATTACTTGTGAGAGTCGTG+TGG 0.661476 8.2:+74110159 MS.gene84662:CDS
ATTAAGTGCAGCATGTTGCG+CGG 0.662601 8.2:-74114391 None:intergenic
TAATGGCATGACCGTCCAAG+AGG 0.670114 8.2:+74111733 MS.gene84662:CDS
TGCGGATGAGATCTTGAGGG+AGG 0.673318 8.2:-74113296 None:intergenic
AGTTATTGGATCCACACATG+AGG 0.685129 8.2:+74113130 MS.gene84662:CDS
ATTCGTCTTTCATATCGGCG+TGG 0.688334 8.2:-74114299 None:intergenic
CCAGACCCAACACAACCACA+TGG 0.693358 8.2:+74114108 MS.gene84662:CDS
ACCTGGTGTTACATGCAGAG+GGG 0.702111 8.2:+74114806 MS.gene84662:CDS
AGTAATAGTTCACATAAGTG+CGG 0.704279 8.2:-74114206 None:intergenic
TTAGAGCTGTTGTGACTGTG+AGG 0.719203 8.2:+74106948 MS.gene84662:CDS
ATTAATCTTCTCATCAACGG+GGG 0.724917 8.2:-74114453 None:intergenic
TATTAATCTTCTCATCAACG+GGG 0.754646 8.2:-74114454 None:intergenic
ATATGAAAGACGAATCATGG+TGG 0.755805 8.2:+74114307 MS.gene84662:CDS
ACAGGCTTTAGAGTACCAAG+TGG 0.757581 8.2:-74112490 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr8.2 gene 74106677 74114851 74106677 ID=MS.gene84662
chr8.2 mRNA 74106677 74114851 74106677 ID=MS.gene84662.t1;Parent=MS.gene84662
chr8.2 exon 74106677 74107082 74106677 ID=MS.gene84662.t1.exon1;Parent=MS.gene84662.t1
chr8.2 CDS 74106677 74107082 74106677 ID=cds.MS.gene84662.t1;Parent=MS.gene84662.t1
chr8.2 exon 74107219 74107499 74107219 ID=MS.gene84662.t1.exon2;Parent=MS.gene84662.t1
chr8.2 CDS 74107219 74107499 74107219 ID=cds.MS.gene84662.t1;Parent=MS.gene84662.t1
chr8.2 exon 74108500 74108740 74108500 ID=MS.gene84662.t1.exon3;Parent=MS.gene84662.t1
chr8.2 CDS 74108500 74108740 74108500 ID=cds.MS.gene84662.t1;Parent=MS.gene84662.t1
chr8.2 exon 74110158 74110493 74110158 ID=MS.gene84662.t1.exon4;Parent=MS.gene84662.t1
chr8.2 CDS 74110158 74110493 74110158 ID=cds.MS.gene84662.t1;Parent=MS.gene84662.t1
chr8.2 exon 74111561 74111754 74111561 ID=MS.gene84662.t1.exon5;Parent=MS.gene84662.t1
chr8.2 CDS 74111561 74111754 74111561 ID=cds.MS.gene84662.t1;Parent=MS.gene84662.t1
chr8.2 exon 74112367 74112668 74112367 ID=MS.gene84662.t1.exon6;Parent=MS.gene84662.t1
chr8.2 CDS 74112367 74112668 74112367 ID=cds.MS.gene84662.t1;Parent=MS.gene84662.t1
chr8.2 exon 74113053 74113319 74113053 ID=MS.gene84662.t1.exon7;Parent=MS.gene84662.t1
chr8.2 CDS 74113053 74113319 74113053 ID=cds.MS.gene84662.t1;Parent=MS.gene84662.t1
chr8.2 exon 74114095 74114851 74114095 ID=MS.gene84662.t1.exon8;Parent=MS.gene84662.t1
chr8.2 CDS 74114095 74114851 74114095 ID=cds.MS.gene84662.t1;Parent=MS.gene84662.t1
Gene Sequence

>MS.gene84662

ATGGCCCTAACAAAAGAATTCATGGGTTCTTCATTGGTAGAGAGACCAATATTTCCTTCTTCTTCACCATGTTTTCAACACAAGAATGGTGGATTCTTGATCAGTCCTGTTTTTGTTCCTTATGGAAACAAAAGAGTTGTAAGGTTGAGAAAATCTGCAAAGTTTCCTGTTGCAGCTATCAGTGAAGATTTGATGAAAAGCTCTTCATCATCTTCTTCTATATCATCATCTTCTTCTTCTGTTCCTGCTGAAAAACCTGTGAAATTTAAGGTTAGAGCTGTTGTGACTGTGAGGAACAAGATCAAAGAAGATTTCAAAGAGACTTTTGTGAAACATCTTGATGCTTTTACTGATAGGATTGGAAGAAATGTTGTTCTTGAACTTTTTAGCACTGAGATTGATCCAAGTAAGACTTTTCTTCATTGTGTTAATTAAAAATCAATATTTACTTTATACACTTAATATACAAACATACACTTAATTTTAATTTCTTTGATTTTGATAATTAAATTATTGGTGATTTTTATGTTGTGATCTTTCAGAAACAAAAGCTGCAAAGAAGACCAATGAAGCAGTGTTGAAGGATTGGTCAAAGAAGACTAATATTAAGGCAGAGAGAGTTAATTACACAGCTGAATTTACGGTTGACTCAAATTTTGGAGAACCAGGAGCTATTACTGTGATAAATAATCATCAGCAAGAATTTTACTTAGAAAATATAACAATTGAAGGATTTGCTACTGGAGCATTTCATTTTCCTTGCAATTCTTGGGTTCAAGCTAGGAAAGATCTTCCTGGAAAGAGAATTTTCTTTTCTAATAAGGTATCTAATAATTAATTCTTCAACTCTTTTTTTGTTATTTATCTGTCATCATAACATGGCCCAGTAAGAAAAAAAAAAGTAGTACAGCCAAAAAAGTAGTATCGTAAGGCAACAATATTTGTTTATTCATTTTTTCAACGTGAGAAATAAGGTGAAAATTGAAAGTTTTTTTTTTGAAATAAGGTGAAAATTGAAAGTTGAAAGGTATAATAATTTTTGTTTTGAAGAATCTTGAAAAGGCACAATAATTCCAAAAGTTCATTTTAACGTAAAAATATTGAATGTTATTTAATAATTATTTTATGTGCCAAAACCAAGCAGTCCGTGATTTTATAATTATTGTTATATATCATTGCATTTTTTAATTAATTATCCACGATAAAGTGATATACTAATAAACAATTAACCTAGAAAATCATATAGTCAAATTTATGCATATGGACCAAGCACCACTGAATTGACCTGACGACTACCTACGGTATGAATTGGGTAAAGACACCGTTGAATTGAATACTAGTAGTACTTGTCAAGAAAAAAAATAATACTAGCAAATACCCTCTTTATCTTTATTTTTCGAGTCAGCAACATTCTTTAACACTCACTCGTTTATTAGATTAAATTAAAGTGGGTCTCGCACGTTGAAAATTGATTTTCTTAAAGTGATGAAATATATATTGATTCCATCTAACAAGAGAGTGAGTATTAGAAACTATTAAAATGAAAGTATCTCTCGCATTTCTCTTATTTTTCTGACTAGTACTAGTAGTCTTTATGGTCGAGGATGGATGTGGAACAATAATCTTACCCTTGGCATTATTCAGCATTTACCTAATGTAAATTTATACAAATAATAAAGTGGTAACATTCCCTAAATAAAAAAATAAAGTGGTAACATTTACCTTTCTCGATCTTTTGACTAAGATCAAGTGTAGTATAAATATTCTTTTCGAAATAAAGTGGTAACATATGTTTGATTTAATGTGCGATTTACTTTGAACAGCCTTATCTACCCGATGATACACCTGCTGGGATTAAACTTTTGAGAGAGAAAGACCTGAAAAATCTGAGAGGTGATGGAAAAGGAGTTAGAAAATTATCTGACAGAATATATGATTATGACACATACAATGATCTTGGAAACCCAGATAAAGGAATTGATCTTGCTAGACCAACTCTTGGAGGATCTGAAATGTATCCATACCCTAGACGGTGCCGTACTGGCCGTGAACCATCTGATACAGGTAATAATTAATTATATAAGCAATTTTACACTACTAATGATTAATGAATATTGCATTGTATCCTTTAGTAGGGTGTTAAACTATATTTCTATCCTTTAGGGTGTGTTTGGTTAAGAAGAGAAGTTGTGAAGAGAGAAATAAGTATGAGAGAGTATGAGAAGAGAGAAAAAGGTGAGAAATAGAATAGATTTGATGTAGTGTTTGGTATAAAAGAAAGAGAAGAGAAACAGAGAAGAGAGAAGTGTTGATTGTTAATAAAAGTACAAAAATAACCTTGATTTTAAAAAAGTTCCTTCACAATAATTTTTTATTAGCTATTTTTATTTTTATTTATTTGTAGGCCTTATTTGAATTATAACTATTTTAGATAATTAAGTTAACTATTGATTTAGTTATTAAATTTTTTATTTTAATCACGTGGTGGGTACATGATGTTGAAGTCTGGAAACAAAAACTACATAACAATGTGTGTGAAACACATGAAACAAAAGGGCAATATTGGAAAATCAAAAATTAAATGGTGCTCTCTCCACTTTCTTTGCTACCTTGGAGAGAAGAAAAAAACATGTGGGACCCAGTCATATTTATTCTCTCTTCGATACTTCTTTGATATGCCAAACGTGAACAGTAACCATTTTCTTTGCATTCTCTCTCGTGCCTTTCTCTCTCGTCCCAACTTCTCGCGTAACAAACACACTGTTAAAGTGCGATGTTAGATGATTCGGGTTTGAACTTCGACTCATTCATTTGTATGTATAAGTAAATTTCCAACGACTATTGTGATTTCATCTATCTAAAAAAAAGTCTTGTTAGAATACTATATGAAACTAAGAAGTTGACAAAAACATAATTTCTCTTTATGAAATTATATGTTGTCCTCGTTTTAGTTGCACATTGTATTCTAAGTTGTATTTAAAGTTATATCTTGTGGTCTTGTAAATGCAATTCAGATGATAAAATTCTAAAATGTTACAATGACATTTGATATGATGGTGTGTGGGCTTAAACTCGCATTTCTCCACTTCTCTATACTTAGTGTGTGTCTATGTACGTGTGAATTTCGCTGCAAATCTCCCTTGGAAAAAAAGTGTGATAGCTTGGAAATATAAAAAATTATTTTACATTCAAATTTAGTAAATTTTTACAAATCAATTTGATTTTAAGGATTAAAATTTATACTAATTTGATGTATTTTCTAACATAGGATCTTCTCTGGACCAACTTGTGGGCACCGACATCTTGTGATGCTTTACAGTGGTGTCATGGCATGCTATAATATTATTTTCTTAAATATTTGTACAAATATTTACTTGATAAAATATGATTATCTGTTTAAAATTATGTAGATTATAATATGTTACTATGCTTTGTGGTGATTCTAAATAACTAAACAAAATTGAATTGGATGGTGCTGCAGATATTACTTGTGAGAGTCGTGTGGAGAAGCCACTTCCTATGTATATACCAAGAGATGAAAGATTTGAGGAATCCAAAATGAACACATTTTCAGTGAAGAGGCTAAAAGGAGTGCTTCATAACTTGCTTCCTGGCCTTAAATCAAGTCTTTCTGCTCAAAACAGAGATTTCAATGAATTCTCAGATGTTGATGGCCTTTATAGTGTTGGACTACTCATTAAGTTGGGTTTGCAAGATGATATTTTGAAGAAACTTCCATTGCCTCACATTGTTAGCAAGATCCAAGAATCCACAAGCCAGGGTATCCTTAAGTATGACATTCCCAAGATTATATCAAGTGAGTGGAATATGCACTTGTTATTGTCGATTTCTTGATTTGCTTGCTAAATAAATTTGGTACATAATTGAGTTAAGCTAATTCTTTTCATAAGTAAATCTTTGGTTTAAATACACTTTTAGTCCTCTCGTCCGATTCACAATTTTGATTCTCCATTTTTTAAATTTTTTGAAAATTTCCGCATTACTGAATTTTGGGCTTAATTTAGCAGTGTGTTAGGTTGAAGAATGAATTAACAAGTCATTGGATGGAAAATGTAGCATTGTATATTTTTTGAGATATAATTTTCTTAGGGTTTTACACTTTCGTCATTTAGAAAATTTCCTCTTTGTATAAAATTTCTAAACTTAATATTTCCAAATTGAATTATGTCTTTATAAAAAATTTAACTTAGATTTGAAATACCTTTAAATCATATACCAATTACATTAAAGGAATATTTATACTTTTTTTACTTTCCCTTTCATTTGCAATGTGGTGGGGTCTTTGGCCATGAATATTCTATAATGGAATGCAAGCATGCCATATGAAAAGATAGTGCTACAAATTAAAGAGTGCCACAGTACAACATGTTATTTGGCTTTTAGTGTTTGTCAGTACAATTTCAACCCAAAAAGATGAGTTTTCAGTTCAAAGCTAGTAGATCTAATGTTTATACCATTCCTTTTATATATAAAAAAATTACAACTGGGCCAATTTGTTATTAATTATTACTTGTTCATTTGAAACTAAACTGTCACCAAAGCTGTACTAATCAATTGTTAATTTTAAATGTTTCACATGTGTGTCTAGTTATTATAAACTGTTGATATAAAAATTAAGGAAAATGCTAACTTGTGCCCTCAAGGCACAAGTTAAGAAACCAAAGATAGAAACATTTTGTTGGAAATTGTGCATTCAATATTATAAAAATTTAAAATCTTTTCTTTTCAAGACATAATTTATGATTGAATTCCATTTTTAGATTCTTAACTTGTGTCCTAAGGGTACAAGTTAACACTACCCAAAAATTAAAGTTCTTGTTTAATTAATTGTGAAATTATTGATCTCATGTGCCTAATGTGCAGAGGATAAATTTGCTTGGTTGAGAGATGATGAATTTGCCCGTCAAGCAATAGCTGGAGTTAATCCTGTTACCATTGAGAGACTTACAGTTTTCCCACCTGTTAGTAAATTAGATCCTGAAATCTATGGTCCACAAGAGTCTGCACTTAAAGAAGAGCACATTTTAAATCAACTTAATGGCATGACCGTCCAAGAGGTACCATTTAAAGCATTCAAAATTTTTTTCCTTTTTGGTACAATGCATTCATAAACTTACTAACCATGTTACCTAATCAAGTGCCTTGTGAATTGTTTTTTCAAACTATTTAACCAATTATAACATTTCCCATGGATTAATTTTTTCACTTGACATCAAATTTATAATGGTCACCAAGTGGTTTGGTTCAAGTAGTGAGTGATTTGGATACTTTAAACAAATGGTCAGAGATTTGACTACTCAGGATCGACCTAAAGACTAAGCAATTTAAGTTAGGGTTTTAAGTCTCACTTCAAAATTTGTTTTAGACTTCTAAACATATTGTGTACGTCCTGAGGCAACTAACTCTTGTATATGGAGAAAAATCAGTTAAAAATGGCGAATCTATCATGGTGCCCAATAGGTTCCAACAAAAATTACCACTGTTAAAGGACAATGATACTTCGTGCCTATAACTTGATTTTAAAGAAATAAATTTAAAATTATCCTATGTATGCTCTTGAATGCACAATTCAATACTAGTAGATCATATAAATTTTGACATTTTTTTTTTATTAATTTACTTATTTGCACTAAACTCCCACTTTCTTTTCTTTTTTTGGAATTTTGCAGGCAATAGATCAAAATAAGTTGTTTATAATTGATTATCATGATATCTACCTTCCATTTTTGGAAAGGATCAATGCCTTGGATGGTAGAAAATCATATGCCACACGTACCATATACTTTTTGACACCACTTGGTACTCTAAAGCCTGTTGCAATTGAACTTAGCCTGCCTCCATCTGGACCAAACACTCGATCAAAACGTGTAGTTACACCTGCTTTAGATGCAACTACTAATTGGGTGTGGATGCTTGCAAAAGCTCATGTTTGCTCCAATGATGCTGGTGTGCACCAACTTGCCCACCATTGGTAAGCAAGCATACAATTTTTCATTTACTTAGTCAATCATCAATTTAGTCCTAAAATTTTATGTGACAAGTGACACAAGACATTTACACTCACACATGGACACTGATAATAATTTAATGAAATAATTGAATATAATCACATATGTTGTATCAAATTGACATGTGTCGGACACAAAATATGTCTTTCATGTGAAAAGACATTGCGACATAGGTTTGAAAAAATTGTTTAGAAATTATTTTGCAATTTTGTTAGTTTCATTGACACAATACCTTGACACATGCAACTTAAATACTAAATTGGTGATTGAAGTACTAGAACTTTTTTTTATATAACTGGCATGAACTTATTTCTTTGCTTATTTTTTCTCGGTGAATTTTTATATAGGCTACGCACACATGCTTGCATGGAACCATTTATATTGTCTGCTCATAGGCAATTAAGTGCAATGCATCCAATTTTCAAGTTATTGGATCCACACATGAGGTACACTTTGGAGATCAATGCTTTAGCTCGACAGAGCTTGATCAATGCAGATGGTGTCATTGAGTCTTGCTTCACTCCTGGTCGCTATGCCATGGAGATTAGTTCTGCTGCTTACAAAACCAACTGGCGTTTTGACCAGGATAGCCTCCCTCAAGATCTCATCCGCAGGTAAATATCATATATTAATTTCGGCATTTTTTTTCCGCTTTGTTTTAAAGATCCTAGAACGAAACTCACACACGCAATACGGAGAATAGACATATTTTTTGCTTCTAACCTCTGTACATCAATTGTTTTCGCTGCCTTTTACCATTTGAGGCGCATTGTTGGTTCGTTATAACTTATTAGTTTATGACCTCAACTCTAAAGTTTAAAATAAGGGTCTGTGATTGTGGTCTGTGATTGTATCAGCTTCGTTTAACTACGATTCTTTGCAATATTAAGGATCGTGACATAACCACTCTCTAAAACCCTCCTCAACTATATTTTGATACCTAAGAGAAATTGTAAATATTAAACTTTCTTTTATGATTTGTTTGAGGCGTCACTTAGTATATGTTTGATTTCATTTTAGTCAAAATTGATTCTAGAAGTGTATAATTGTTTTTTATATGTTTGAGTGCATTTCGGAATAATTGATTTTGACTCTAGAATTGATTTTAATTTGAAACTATGATTTATAGTTTTTGACTCTAAACGTTAATTTTACACTTAAATTTATTGTTCAATCCACAATATGTGAATTTGTCCAAACGTAAACCATTTTACATTCAACTTAGTTTTAACTAAAATCAATTTTTCTCACCATAAAATCAAATACACACCTAGCTTCTACGGAAAAGAGACCCTACACTTTTCTTAACATGTAATTGATGTTTGCTTTATTTGGTGCAAGCATATGACATATAAATCTAACTAAGATTTTCACTTTTTAACATTTCAGGGGAATGGCAGTACCAGACCCAACACAACCACATGGTCTAAAGCTAATAATGAAAGATTACCCTTATGCAGAAGATGGTCTTCTAATTTGGTCAGCAATAGAAAATTGGGTCCGCACTTATGTGAACTATTACTACCCAAATCCAAGCCTAATAATCAATGACAGAGAGTTACAAGCTTGGTACTCCGAATCAATCAATGTAGGCCACGCCGATATGAAAGACGAATCATGGTGGCCAAAATTGAACGACAGTGACAATCTTGTCCAAGTCCTAACAATCTTAATTTGGAATGCATCCGCGCAACATGCTGCACTTAATTTTGGACAGTACCCTTATGGAGGGTACGTGCCAAATCGTCCCCCGTTGATGAGAAGATTAATACCGGAAGAAAATGACCCTGAATATACAAGTTTCATATCTGATCCACAAAAGTATTTTCTCAATGCATTGCCTAGTTTGTTGCAAGCTACAAAATATATGGCTGTTGTTGACACGCTTTCGACTCATTCGTCTGACGAAGAGTATTTAGGTGAGAGGCAACAGCCTTCGATTTGGACGGGTGATGCTGAGATTGTCGAAGCATTTTATCAATTCTCTGCTGAAATTGGAAAGATCGAGAAGGTGATTGATAGTAGGAATGGTGATAGGAATTTGAGGAACCGTTGCGGTGCTGGTGTTTTGCCTTATGAATTACTTGCTCCTAGTTCTGGACCTGGTGTTACATGCAGAGGGGTTCCAAATAGTGTGTCCACATAA
Protein sequence

>MS.gene84662.t1

MALTKEFMGSSLVERPIFPSSSPCFQHKNGGFLISPVFVPYGNKRVVRLRKSAKFPVAAISEDLMKSSSSSSSISSSSSSVPAEKPVKFKVRAVVTVRNKIKEDFKETFVKHLDAFTDRIGRNVVLELFSTEIDPKTKAAKKTNEAVLKDWSKKTNIKAERVNYTAEFTVDSNFGEPGAITVINNHQQEFYLENITIEGFATGAFHFPCNSWVQARKDLPGKRIFFSNKPYLPDDTPAGIKLLREKDLKNLRGDGKGVRKLSDRIYDYDTYNDLGNPDKGIDLARPTLGGSEMYPYPRRCRTGREPSDTDITCESRVEKPLPMYIPRDERFEESKMNTFSVKRLKGVLHNLLPGLKSSLSAQNRDFNEFSDVDGLYSVGLLIKLGLQDDILKKLPLPHIVSKIQESTSQGILKYDIPKIISKDKFAWLRDDEFARQAIAGVNPVTIERLTVFPPVSKLDPEIYGPQESALKEEHILNQLNGMTVQEAIDQNKLFIIDYHDIYLPFLERINALDGRKSYATRTIYFLTPLGTLKPVAIELSLPPSGPNTRSKRVVTPALDATTNWVWMLAKAHVCSNDAGVHQLAHHWLRTHACMEPFILSAHRQLSAMHPIFKLLDPHMRYTLEINALARQSLINADGVIESCFTPGRYAMEISSAAYKTNWRFDQDSLPQDLIRRGMAVPDPTQPHGLKLIMKDYPYAEDGLLIWSAIENWVRTYVNYYYPNPSLIINDRELQAWYSESINVGHADMKDESWWPKLNDSDNLVQVLTILIWNASAQHAALNFGQYPYGGYVPNRPPLMRRLIPEENDPEYTSFISDPQKYFLNALPSLLQATKYMAVVDTLSTHSSDEEYLGERQQPSIWTGDAEIVEAFYQFSAEIGKIEKVIDSRNGDRNLRNRCGAGVLPYELLAPSSGPGVTCRGVPNSVST