AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene84718

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84718.t1 MTR_8g024790 94.260 453 24 1 12 462 12 464 0.0 889
MS.gene84718.t1 MTR_8g024770 79.630 432 67 8 38 460 35 454 0.0 682
MS.gene84718.t1 MTR_5g030430 50.990 202 88 4 270 461 474 674 5.80e-55 194
MS.gene84718.t1 MTR_5g030430 32.973 185 91 4 25 180 44 224 3.78e-28 118
MS.gene84718.t1 MTR_8g067280 48.020 202 92 4 271 461 444 643 1.95e-51 184
MS.gene84718.t1 MTR_8g067280 31.282 195 101 6 25 190 53 243 7.45e-25 108
MS.gene84718.t1 MTR_7g096350 28.631 482 241 15 19 440 43 481 2.65e-41 154
MS.gene84718.t1 MTR_7g117670 26.265 514 263 14 25 440 49 544 3.44e-37 144
MS.gene84718.t1 MTR_5g030420 31.720 186 94 4 25 181 27 208 7.50e-29 114
MS.gene84718.t1 MTR_5g030420 31.720 186 94 4 25 181 27 208 9.81e-29 114
MS.gene84718.t1 MTR_8g027495 37.975 158 89 1 283 440 452 600 1.50e-27 116
MS.gene84718.t1 MTR_1g072320 33.526 173 103 3 281 441 457 629 1.24e-18 89.4
MS.gene84718.t1 MTR_1g072320 27.638 199 94 7 19 181 4 188 1.44e-16 82.8
MS.gene84718.t1 MTR_4g097940 30.500 200 111 7 243 426 102 289 6.09e-18 85.1
MS.gene84718.t1 MTR_4g009110 27.363 201 100 5 252 426 133 313 3.48e-17 83.2
MS.gene84718.t1 MTR_2g104490 29.444 180 113 6 272 441 116 291 7.77e-17 81.3
MS.gene84718.t1 MTR_3g104100 33.939 165 98 4 277 431 183 346 1.34e-16 81.6
MS.gene84718.t1 MTR_2g104490 30.625 160 102 5 272 426 116 271 2.22e-16 80.1
MS.gene84718.t1 MTR_0250s0040 28.729 181 109 5 289 457 88 260 3.15e-16 79.0
MS.gene84718.t1 MTR_4g009540 30.435 161 95 5 275 426 142 294 3.55e-16 79.7
MS.gene84718.t1 MTR_4g009540 30.435 161 95 5 275 426 142 294 5.82e-16 79.3
MS.gene84718.t1 MTR_2g104550 25.000 160 114 2 272 426 117 275 5.92e-16 79.0
MS.gene84718.t1 MTR_5g014640 30.899 178 108 5 290 459 155 325 6.72e-16 79.0
MS.gene84718.t1 MTR_6g047570 32.168 143 90 3 260 401 134 270 9.53e-16 78.6
MS.gene84718.t1 MTR_5g014600 28.063 253 148 10 183 418 52 287 2.61e-15 77.4
MS.gene84718.t1 MTR_4g092700 28.161 174 103 4 263 428 137 296 3.53e-15 77.0
MS.gene84718.t1 MTR_1g112680 27.753 227 123 4 241 428 259 483 3.78e-15 78.2
MS.gene84718.t1 MTR_0011s0260 31.159 138 94 1 289 426 183 319 4.01e-15 77.0
MS.gene84718.t1 MTR_4g066460 28.313 166 103 3 262 426 138 288 4.92e-15 76.3
MS.gene84718.t1 MTR_0011s0210 30.216 139 94 2 289 426 183 319 9.55e-15 75.9
MS.gene84718.t1 MTR_2g022280 26.136 176 116 4 288 451 1 174 9.76e-15 73.2
MS.gene84718.t1 MTR_2g081040 35.032 157 87 4 286 430 174 327 1.03e-14 75.9
MS.gene84718.t1 MTR_4g067010 26.857 175 107 5 258 426 121 280 1.08e-14 75.5
MS.gene84718.t1 MTR_4g097920 28.643 199 115 7 243 428 102 286 1.53e-14 75.1
MS.gene84718.t1 MTR_4g097920 28.643 199 115 7 243 428 87 271 1.81e-14 74.3
MS.gene84718.t1 MTR_3g030700 31.792 173 108 4 266 431 173 342 2.01e-14 75.1
MS.gene84718.t1 MTR_8g098275 38.835 103 53 2 288 380 307 409 7.90e-14 73.9
MS.gene84718.t1 MTR_8g098275 38.835 103 53 2 288 380 243 345 8.10e-14 73.6
MS.gene84718.t1 MTR_8g098275 38.835 103 53 2 288 380 400 502 8.59e-14 73.9
MS.gene84718.t1 MTR_7g083900 60.000 55 22 0 287 341 284 338 1.01e-13 73.6
MS.gene84718.t1 MTR_3g498695 58.182 55 23 0 287 341 296 350 1.02e-13 73.6
MS.gene84718.t1 MTR_7g083900 60.000 55 22 0 287 341 284 338 1.02e-13 73.6
MS.gene84718.t1 MTR_3g498695 58.182 55 23 0 287 341 296 350 1.04e-13 73.6
MS.gene84718.t1 MTR_2g010450 30.345 145 85 3 289 425 177 313 1.84e-13 72.0
MS.gene84718.t1 MTR_4g066380 25.728 206 131 6 263 462 137 326 1.94e-13 71.6
MS.gene84718.t1 MTR_6g047550 37.838 111 61 2 260 370 148 250 1.99e-13 70.9
MS.gene84718.t1 MTR_4g097950 30.714 140 87 3 285 418 67 202 4.44e-13 69.3
MS.gene84718.t1 MTR_2g038040 53.226 62 22 1 290 344 53 114 5.11e-13 68.9
MS.gene84718.t1 MTR_0246s0020 49.254 67 30 1 289 355 198 260 8.22e-13 70.1
MS.gene84718.t1 MTR_0246s0020 49.254 67 30 1 289 355 198 260 8.35e-13 70.1
MS.gene84718.t1 MTR_2g104530 28.777 139 94 1 288 426 104 237 9.12e-13 68.9
MS.gene84718.t1 MTR_8g009020 25.316 158 112 2 275 426 129 286 3.77e-12 67.8
MS.gene84718.t1 MTR_4g094762 29.012 162 84 5 290 431 293 443 7.11e-12 67.8
MS.gene84718.t1 MTR_2g104500 25.806 186 130 5 241 426 58 235 2.44e-11 64.7
MS.gene84718.t1 MTR_4g057270 30.000 160 71 7 293 431 153 292 3.76e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84718.t1 AT4G00870 37.527 461 200 14 20 460 29 421 9.81e-73 236
MS.gene84718.t1 AT4G17880 28.864 537 270 13 26 462 63 587 2.94e-68 228
MS.gene84718.t1 AT5G46830 32.952 525 238 17 18 460 18 510 1.06e-64 217
MS.gene84718.t1 AT1G32640 51.980 202 89 4 263 461 427 623 1.49e-56 198
MS.gene84718.t1 AT1G32640 31.579 190 93 3 25 181 67 252 2.40e-30 124
MS.gene84718.t1 AT5G46760 44.770 239 120 5 229 462 359 590 6.90e-54 190
MS.gene84718.t1 AT5G46760 32.065 184 89 5 25 176 51 230 1.81e-26 112
MS.gene84718.t1 AT4G16430 27.215 474 262 13 21 462 45 467 9.20e-41 152
MS.gene84718.t1 AT2G46510 41.714 175 82 3 269 443 376 530 2.22e-34 135
MS.gene84718.t1 AT1G01260 38.889 180 85 3 273 452 417 571 3.17e-30 124
MS.gene84718.t1 AT1G01260 28.571 196 99 5 26 184 48 239 3.74e-13 72.0
MS.gene84718.t1 AT1G01260 38.889 180 85 3 273 452 417 571 3.17e-30 124
MS.gene84718.t1 AT1G01260 28.571 196 99 5 26 184 48 239 3.74e-13 72.0
MS.gene84718.t1 AT1G01260 38.889 180 85 3 273 452 417 571 3.17e-30 124
MS.gene84718.t1 AT1G01260 28.571 196 99 5 26 184 48 239 3.74e-13 72.0
MS.gene84718.t1 AT4G09820 22.998 487 246 16 27 417 17 470 3.01e-24 105
MS.gene84718.t1 AT4G00480 22.201 518 296 15 31 461 26 523 1.43e-19 91.7
MS.gene84718.t1 AT4G00480 22.549 510 294 13 31 456 26 518 2.76e-19 91.3
MS.gene84718.t1 AT4G00480 22.691 498 278 15 31 441 26 503 3.17e-19 90.5
MS.gene84718.t1 AT1G63650 26.486 185 119 3 289 456 405 589 2.90e-16 81.6
MS.gene84718.t1 AT1G63650 26.486 185 119 3 289 456 405 589 2.90e-16 81.6
MS.gene84718.t1 AT1G63650 26.486 185 119 3 289 456 405 589 2.90e-16 81.6
MS.gene84718.t1 AT4G37850 31.325 166 104 5 266 426 130 290 4.01e-16 79.7
MS.gene84718.t1 AT4G37850 31.325 166 104 5 266 426 130 290 4.01e-16 79.7
MS.gene84718.t1 AT3G26744 60.000 55 22 0 287 341 305 359 1.72e-14 75.9
MS.gene84718.t1 AT1G12860 57.895 57 24 0 287 343 265 321 1.78e-14 75.5
MS.gene84718.t1 AT3G26744 60.000 55 22 0 287 341 305 359 1.78e-14 75.9
MS.gene84718.t1 AT3G26744 60.000 55 22 0 287 341 305 359 1.78e-14 75.9
MS.gene84718.t1 AT3G26744 60.000 55 22 0 287 341 305 359 1.78e-14 75.9
MS.gene84718.t1 AT1G12860 57.895 57 24 0 287 343 265 321 2.11e-14 75.5
MS.gene84718.t1 AT5G57150 26.908 249 138 10 233 461 7 231 7.68e-14 71.6
MS.gene84718.t1 AT5G57150 27.193 228 128 8 233 441 7 215 1.34e-13 70.5
MS.gene84718.t1 AT5G57150 27.468 233 126 10 233 443 7 218 1.71e-13 70.9
MS.gene84718.t1 AT3G06120 27.869 183 111 4 288 449 1 183 1.78e-13 69.7
MS.gene84718.t1 AT5G57150 26.754 228 129 8 233 441 7 215 2.14e-13 69.7
MS.gene84718.t1 AT4G29930 54.098 61 21 1 290 343 55 115 2.26e-13 70.1
MS.gene84718.t1 AT4G29930 54.098 61 21 1 290 343 55 115 2.60e-13 69.7
MS.gene84718.t1 AT4G29930 54.098 61 21 1 290 343 55 115 2.81e-13 69.7
MS.gene84718.t1 AT5G57150 36.719 128 59 5 233 359 7 113 2.99e-13 69.3
MS.gene84718.t1 AT4G29930 55.000 60 20 1 291 343 56 115 4.22e-13 68.2
MS.gene84718.t1 AT4G29930 54.098 61 21 1 290 343 55 115 4.76e-13 69.7
MS.gene84718.t1 AT4G29930 54.098 61 21 1 290 343 55 115 4.76e-13 69.7
MS.gene84718.t1 AT4G29930 54.098 61 21 1 290 343 55 115 5.24e-13 69.3
MS.gene84718.t1 AT1G10610 32.847 137 85 4 290 421 235 369 1.89e-12 69.3
MS.gene84718.t1 AT1G10610 32.847 137 85 4 290 421 265 399 2.22e-12 69.3
MS.gene84718.t1 AT1G10610 32.847 137 85 4 290 421 168 302 2.34e-12 68.6
MS.gene84718.t1 AT5G41315 32.258 93 49 1 289 381 449 527 2.77e-12 69.3
MS.gene84718.t1 AT5G41315 32.258 93 49 1 289 381 446 524 2.81e-12 69.3
MS.gene84718.t1 AT5G41315 32.258 93 49 1 289 381 441 519 2.87e-12 69.3
MS.gene84718.t1 AT2G22760 28.415 183 106 7 276 439 100 276 6.83e-12 66.6
MS.gene84718.t1 AT2G16910 54.000 50 23 0 293 342 318 367 1.13e-11 67.4
MS.gene84718.t1 AT3G24140 23.721 215 137 4 269 456 175 389 1.63e-11 66.2
MS.gene84718.t1 AT2G22760 29.697 165 96 6 276 426 100 258 1.91e-11 65.1
MS.gene84718.t1 AT3G24140 23.721 215 137 4 269 456 175 389 2.36e-11 65.9

Find 77 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTCATTTGTTGCAAATTT+TGG 0.152440 8.3:-67651780 None:intergenic
TCCAAACCCAATCCGAAAAT+TGG 0.161848 8.3:+67651194 MS.gene84718:CDS
ATTGAAGGATTTGGAGTTTC+AGG 0.165432 8.3:+67652332 MS.gene84718:CDS
GAGATTGATGTGAAGATAAT+AGG 0.216324 8.3:+67652243 MS.gene84718:CDS
AAGGTAAACCTTTCTTATCT+TGG 0.238310 8.3:+67651254 MS.gene84718:CDS
GTTTGAAATTGCTGTTTCTT+TGG 0.265579 8.3:-67651299 None:intergenic
ATGACAATATCCAACAAAAT+TGG 0.270722 8.3:+67651584 MS.gene84718:CDS
TTATCCATCTTTGAAACATT+TGG 0.290544 8.3:-67652030 None:intergenic
AGGTAAACCTTTCTTATCTT+GGG 0.292720 8.3:+67651255 MS.gene84718:CDS
ATCTTAAAGCATAGAATCTA+TGG 0.304209 8.3:-67651999 None:intergenic
TTGCTGTTTCTTTGGTTCCT+TGG 0.308281 8.3:-67651291 None:intergenic
AAGATTGAAGATTTAGAATC+AGG 0.311502 8.3:+67652102 MS.gene84718:CDS
AATGAGTGTATTGAAGGATT+TGG 0.327266 8.3:+67652323 MS.gene84718:CDS
TACCGATGTCGGCGAATGTT+TGG 0.330440 8.3:-67651708 None:intergenic
ATTCTTGTTGGTGTTGCTAA+TGG 0.335278 8.3:-67651859 None:intergenic
TCCATGCACGTGTGCTTCAT+TGG 0.351119 8.3:-67651506 None:intergenic
AGGGTTAGGGTTTGTGGTGA+AGG 0.352816 8.3:-67651148 None:intergenic
TGTTGAAGGGTTAGGGTTTG+TGG 0.360355 8.3:-67651154 None:intergenic
AACTAACGGTGTAATCGAAA+TGG 0.368563 8.3:+67651555 MS.gene84718:CDS
ATCAGTGTCTTCTCCGGAAC+CGG 0.379047 8.3:-67651737 None:intergenic
AGAGGAAGAAAACCGTTATC+AGG 0.381422 8.3:+67651919 MS.gene84718:CDS
TCAGCCATTCTCATGAAATT+AGG 0.386390 8.3:+67652453 MS.gene84718:CDS
TAGATTCTGATGTGGATGTT+TGG 0.396023 8.3:-67651636 None:intergenic
ATCGACTGTTAATGAAGTTA+TGG 0.398979 8.3:+67652371 MS.gene84718:CDS
TAGATTTGGCTTGGTGTACT+AGG 0.400415 8.3:-67651609 None:intergenic
CAAACAACAAAAGATGAAAA+AGG 0.404302 8.3:+67651235 MS.gene84718:CDS
ATCGGTATCGTCTCCGGTTC+CGG 0.406820 8.3:+67651724 MS.gene84718:CDS
GGATGTTTGGAACATAGATT+TGG 0.408652 8.3:-67651623 None:intergenic
TCTGCTTCCACATGGTTTAA+CGG 0.415651 8.3:-67651952 None:intergenic
AAGATTAATGAGTGTATTGA+AGG 0.416800 8.3:+67652317 MS.gene84718:CDS
GGGTTAGGGTTTGTGGTGAA+GGG 0.421882 8.3:-67651147 None:intergenic
TTGCTTTAGATTCTGTACTT+TGG 0.425852 8.3:+67651440 MS.gene84718:CDS
AGATTTGTCAAGTGGGTTTG+TGG 0.429194 8.3:-67651680 None:intergenic
GGTTCCAAATGTTTCAAAGA+TGG 0.435449 8.3:+67652026 MS.gene84718:CDS
AAAAGTGAAGATGGAAATGA+TGG 0.445716 8.3:+67652143 MS.gene84718:CDS
CCTTTCTTATCTTGGGGTGA+AGG 0.446476 8.3:+67651262 MS.gene84718:CDS
TCTTCCGCGCTCATCTCGTT+AGG 0.463829 8.3:-67652417 None:intergenic
CCAAACCCAATCCGAAAATT+GGG 0.468035 8.3:+67651195 MS.gene84718:CDS
TGTCTCATCAGTGTCTTCTC+CGG 0.468594 8.3:-67651743 None:intergenic
GACAGCAACACGTGTACAAA+TGG 0.479858 8.3:+67651331 MS.gene84718:CDS
CTGACCTAATTTCATGAGAA+TGG 0.482002 8.3:-67652457 None:intergenic
GCTTCTTCAATTTCATTACC+TGG 0.483921 8.3:+67651409 MS.gene84718:CDS
ACTAACGGTGTAATCGAAAT+GGG 0.485666 8.3:+67651556 MS.gene84718:CDS
CCTTCACCCCAAGATAAGAA+AGG 0.497048 8.3:-67651262 None:intergenic
TCGATTACACCGTTAGTTGT+CGG 0.498052 8.3:-67651550 None:intergenic
ATTTGTATTCCGACAACTAA+CGG 0.507792 8.3:+67651541 MS.gene84718:CDS
GGTGTTTGAGTTCCTGATAA+CGG 0.508977 8.3:-67651931 None:intergenic
GCCGACATCGGTATCGTCTC+CGG 0.512586 8.3:+67651718 MS.gene84718:CDS
TTTAGAATCAGGGCAACAAA+AGG 0.514365 8.3:+67652113 MS.gene84718:CDS
TTTGTCTCTCTGCTTCCACA+TGG 0.518910 8.3:-67651960 None:intergenic
TCCAATGAAGCACACGTGCA+TGG 0.524272 8.3:+67651505 MS.gene84718:CDS
TGGAACATAGATTTGTCAAG+TGG 0.527748 8.3:-67651688 None:intergenic
ATTCTATGCTTTAAGATCTG+TGG 0.527981 8.3:+67652005 MS.gene84718:CDS
ACACTCATTAATCTTGCACT+AGG 0.528247 8.3:-67652309 None:intergenic
AATTGAAGAAGCATTACCTA+TGG 0.532558 8.3:-67651398 None:intergenic
TTTGGAACATAGATTTGGCT+TGG 0.537383 8.3:-67651618 None:intergenic
GGTAAACCTTTCTTATCTTG+GGG 0.537701 8.3:+67651256 MS.gene84718:CDS
TTCCAAACATTCGCCGACAT+CGG 0.537863 8.3:+67651706 MS.gene84718:CDS
ATGTGGAAGCAGAGAGACAA+AGG 0.547229 8.3:+67651962 MS.gene84718:CDS
ATCATAGAGGAAGATGAAGA+AGG 0.553296 8.3:-67651100 None:intergenic
TCAAAAGCATTAACATTACA+AGG 0.560113 8.3:-67652222 None:intergenic
AGATTGAAGATTTAGAATCA+GGG 0.563283 8.3:+67652103 MS.gene84718:CDS
CTTGTTGGTGTTGCTAATGG+TGG 0.567083 8.3:-67651856 None:intergenic
GATAATAGGAAATGATGCTA+TGG 0.577854 8.3:+67652257 MS.gene84718:CDS
TGCAACGGCAACTTCAACTG+TGG 0.578390 8.3:+67652185 MS.gene84718:CDS
TGGGGTGAAGGCTATTTCCA+AGG 0.580662 8.3:+67651274 MS.gene84718:CDS
GGAACATAGATTTGTCAAGT+GGG 0.591339 8.3:-67651687 None:intergenic
GTACAAATGGTGATGCTGAA+TGG 0.602234 8.3:+67651344 MS.gene84718:CDS
TCTAAAGCAAAAGCTTTACC+AGG 0.602957 8.3:-67651427 None:intergenic
AAAAGAGAACCTAAGAAGAG+AGG 0.611347 8.3:+67651901 MS.gene84718:CDS
TTCTGAGTTAGATTCTGATG+TGG 0.614919 8.3:-67651644 None:intergenic
TGTCGATAATAACAGTGCAA+CGG 0.631393 8.3:+67652170 MS.gene84718:CDS
CACTCATTAATCTTGCACTA+GGG 0.639299 8.3:-67652308 None:intergenic
ATGAGGAAATGATATCATAG+AGG 0.640466 8.3:-67651113 None:intergenic
ACCGGAGACGATACCGATGT+CGG 0.645053 8.3:-67651719 None:intergenic
TCAAACACCGTTAAACCATG+TGG 0.685029 8.3:+67651945 MS.gene84718:CDS
AGTTCCTAACGAGATGAGCG+CGG 0.706023 8.3:+67652413 MS.gene84718:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATTGAAGTTTTTGTTGA+AGG - chr8.3:67651171-67651190 None:intergenic 20.0%
!!! TAATTGAAGTTTTTGTTGAA+GGG - chr8.3:67651170-67651189 None:intergenic 20.0%
!!! TATTCATTTGTTGCAAATTT+TGG - chr8.3:67651783-67651802 None:intergenic 20.0%
! AAGATTAATGAGTGTATTGA+AGG + chr8.3:67652317-67652336 MS.gene84718:CDS 25.0%
! ATCTTAAAGCATAGAATCTA+TGG - chr8.3:67652002-67652021 None:intergenic 25.0%
! ATGACAATATCCAACAAAAT+TGG + chr8.3:67651584-67651603 MS.gene84718:CDS 25.0%
! CAAACAACAAAAGATGAAAA+AGG + chr8.3:67651235-67651254 MS.gene84718:CDS 25.0%
!! AAGATTGAAGATTTAGAATC+AGG + chr8.3:67652102-67652121 MS.gene84718:CDS 25.0%
!! AGATTGAAGATTTAGAATCA+GGG + chr8.3:67652103-67652122 MS.gene84718:CDS 25.0%
!!! GAATCTTTTTCAATTCTTGT+TGG - chr8.3:67651874-67651893 None:intergenic 25.0%
!!! TAACATTACAAGGTCTTTTT+TGG - chr8.3:67652215-67652234 None:intergenic 25.0%
!!! TCAAAAGCATTAACATTACA+AGG - chr8.3:67652225-67652244 None:intergenic 25.0%
!!! TTATCCATCTTTGAAACATT+TGG - chr8.3:67652033-67652052 None:intergenic 25.0%
AAAAGTGAAGATGGAAATGA+TGG + chr8.3:67652143-67652162 MS.gene84718:CDS 30.0%
AAGGTAAACCTTTCTTATCT+TGG + chr8.3:67651254-67651273 MS.gene84718:CDS 30.0%
AATGAGTGTATTGAAGGATT+TGG + chr8.3:67652323-67652342 MS.gene84718:CDS 30.0%
AGGTAAACCTTTCTTATCTT+GGG + chr8.3:67651255-67651274 MS.gene84718:CDS 30.0%
ATCGACTGTTAATGAAGTTA+TGG + chr8.3:67652371-67652390 MS.gene84718:CDS 30.0%
ATGAGGAAATGATATCATAG+AGG - chr8.3:67651116-67651135 None:intergenic 30.0%
ATTTGTATTCCGACAACTAA+CGG + chr8.3:67651541-67651560 MS.gene84718:CDS 30.0%
GAGATTGATGTGAAGATAAT+AGG + chr8.3:67652243-67652262 MS.gene84718:CDS 30.0%
GATAATAGGAAATGATGCTA+TGG + chr8.3:67652257-67652276 MS.gene84718:CDS 30.0%
! AAGTTTTTGTTGAAGGGTTA+GGG - chr8.3:67651164-67651183 None:intergenic 30.0%
! TTGAGTCGAAATTTTTCCAT+AGG + chr8.3:67651382-67651401 MS.gene84718:CDS 30.0%
! TTGCTTTAGATTCTGTACTT+TGG + chr8.3:67651440-67651459 MS.gene84718:CDS 30.0%
!! AATTGAAGAAGCATTACCTA+TGG - chr8.3:67651401-67651420 None:intergenic 30.0%
!! ATTCTATGCTTTAAGATCTG+TGG + chr8.3:67652005-67652024 MS.gene84718:CDS 30.0%
!! GTTTGAAATTGCTGTTTCTT+TGG - chr8.3:67651302-67651321 None:intergenic 30.0%
!!! TTTTGTTGTTTGCCACAAAA+TGG - chr8.3:67651227-67651246 None:intergenic 30.0%
AAAAGAGAACCTAAGAAGAG+AGG + chr8.3:67651901-67651920 MS.gene84718:CDS 35.0%
AACTAACGGTGTAATCGAAA+TGG + chr8.3:67651555-67651574 MS.gene84718:CDS 35.0%
ACACTCATTAATCTTGCACT+AGG - chr8.3:67652312-67652331 None:intergenic 35.0%
ACTAACGGTGTAATCGAAAT+GGG + chr8.3:67651556-67651575 MS.gene84718:CDS 35.0%
ATCATAGAGGAAGATGAAGA+AGG - chr8.3:67651103-67651122 None:intergenic 35.0%
CACTCATTAATCTTGCACTA+GGG - chr8.3:67652311-67652330 None:intergenic 35.0%
CTGACCTAATTTCATGAGAA+TGG - chr8.3:67652460-67652479 None:intergenic 35.0%
GCTTCTTCAATTTCATTACC+TGG + chr8.3:67651409-67651428 MS.gene84718:CDS 35.0%
GGAACATAGATTTGTCAAGT+GGG - chr8.3:67651690-67651709 None:intergenic 35.0%
GGAATCGAAAAAAGTGAAGA+TGG + chr8.3:67652134-67652153 MS.gene84718:CDS 35.0%
GGTAAACCTTTCTTATCTTG+GGG + chr8.3:67651256-67651275 MS.gene84718:CDS 35.0%
GGTTCCAAATGTTTCAAAGA+TGG + chr8.3:67652026-67652045 MS.gene84718:CDS 35.0%
TAGATTCTGATGTGGATGTT+TGG - chr8.3:67651639-67651658 None:intergenic 35.0%
TCAGCCATTCTCATGAAATT+AGG + chr8.3:67652453-67652472 MS.gene84718:CDS 35.0%
TGGAACATAGATTTGTCAAG+TGG - chr8.3:67651691-67651710 None:intergenic 35.0%
TGTCGATAATAACAGTGCAA+CGG + chr8.3:67652170-67652189 MS.gene84718:CDS 35.0%
TTCTGAGTTAGATTCTGATG+TGG - chr8.3:67651647-67651666 None:intergenic 35.0%
! AATGGCATAGACCCAATTTT+CGG - chr8.3:67651209-67651228 None:intergenic 35.0%
! ATAGACCCAATTTTCGGATT+GGG - chr8.3:67651203-67651222 None:intergenic 35.0%
! ATTGAAGGATTTGGAGTTTC+AGG + chr8.3:67652332-67652351 MS.gene84718:CDS 35.0%
! GAAGGGTTTTCATTTTCATG+AGG - chr8.3:67651133-67651152 None:intergenic 35.0%
! GAAGTTTTTGTTGAAGGGTT+AGG - chr8.3:67651165-67651184 None:intergenic 35.0%
! GGATGTTTGGAACATAGATT+TGG - chr8.3:67651626-67651645 None:intergenic 35.0%
! GTACTAGGTTCCAATTTTGT+TGG - chr8.3:67651597-67651616 None:intergenic 35.0%
! TCTAAAGCAAAAGCTTTACC+AGG - chr8.3:67651430-67651449 None:intergenic 35.0%
! TTCTCTTTTATCAGATGCAG+TGG + chr8.3:67652056-67652075 MS.gene84718:CDS 35.0%
! TTTAGAATCAGGGCAACAAA+AGG + chr8.3:67652113-67652132 MS.gene84718:CDS 35.0%
! TTTGGAACATAGATTTGGCT+TGG - chr8.3:67651621-67651640 None:intergenic 35.0%
!! ATTCTTGTTGGTGTTGCTAA+TGG - chr8.3:67651862-67651881 None:intergenic 35.0%
AGAGGAAGAAAACCGTTATC+AGG + chr8.3:67651919-67651938 MS.gene84718:CDS 40.0%
CCAAACCCAATCCGAAAATT+GGG + chr8.3:67651195-67651214 MS.gene84718:CDS 40.0%
TCAAACACCGTTAAACCATG+TGG + chr8.3:67651945-67651964 MS.gene84718:CDS 40.0%
TCCAAACCCAATCCGAAAAT+TGG + chr8.3:67651194-67651213 MS.gene84718:CDS 40.0%
TCGATTACACCGTTAGTTGT+CGG - chr8.3:67651553-67651572 None:intergenic 40.0%
TCTGCTTCCACATGGTTTAA+CGG - chr8.3:67651955-67651974 None:intergenic 40.0%
! AGATTTGTCAAGTGGGTTTG+TGG - chr8.3:67651683-67651702 None:intergenic 40.0%
! CATAGACCCAATTTTCGGAT+TGG - chr8.3:67651204-67651223 None:intergenic 40.0%
! GGTGTTTGAGTTCCTGATAA+CGG - chr8.3:67651934-67651953 None:intergenic 40.0%
! GGTTTTCTTCCTCTCTTCTT+AGG - chr8.3:67651913-67651932 None:intergenic 40.0%
! GTACAAATGGTGATGCTGAA+TGG + chr8.3:67651344-67651363 MS.gene84718:CDS 40.0%
!! TAGATTTGGCTTGGTGTACT+AGG - chr8.3:67651612-67651631 None:intergenic 40.0%
!! TTGCTGTTTCTTTGGTTCCT+TGG - chr8.3:67651294-67651313 None:intergenic 40.0%
!!! ATTGGGTCTATGCCATTTTG+TGG + chr8.3:67651212-67651231 MS.gene84718:CDS 40.0%
ATGTGGAAGCAGAGAGACAA+AGG + chr8.3:67651962-67651981 MS.gene84718:CDS 45.0%
CCTTCACCCCAAGATAAGAA+AGG - chr8.3:67651265-67651284 None:intergenic 45.0%
CCTTTCTTATCTTGGGGTGA+AGG + chr8.3:67651262-67651281 MS.gene84718:CDS 45.0%
GACAGCAACACGTGTACAAA+TGG + chr8.3:67651331-67651350 MS.gene84718:CDS 45.0%
TGTCTCATCAGTGTCTTCTC+CGG - chr8.3:67651746-67651765 None:intergenic 45.0%
TGTTGAAGGGTTAGGGTTTG+TGG - chr8.3:67651157-67651176 None:intergenic 45.0%
TTCCAAACATTCGCCGACAT+CGG + chr8.3:67651706-67651725 MS.gene84718:CDS 45.0%
TTTGTCTCTCTGCTTCCACA+TGG - chr8.3:67651963-67651982 None:intergenic 45.0%
!! CCCAATTTTCGGATTGGGTT+TGG - chr8.3:67651198-67651217 None:intergenic 45.0%
!! CTTGTTGGTGTTGCTAATGG+TGG - chr8.3:67651859-67651878 None:intergenic 45.0%
AGGGTTAGGGTTTGTGGTGA+AGG - chr8.3:67651151-67651170 None:intergenic 50.0%
AGTTCCTAACGAGATGAGCG+CGG + chr8.3:67652413-67652432 MS.gene84718:CDS 50.0%
ATCAGTGTCTTCTCCGGAAC+CGG - chr8.3:67651740-67651759 None:intergenic 50.0%
GGGTTAGGGTTTGTGGTGAA+GGG - chr8.3:67651150-67651169 None:intergenic 50.0%
TACCGATGTCGGCGAATGTT+TGG - chr8.3:67651711-67651730 None:intergenic 50.0%
TCCAATGAAGCACACGTGCA+TGG + chr8.3:67651505-67651524 MS.gene84718:CDS 50.0%
TCCATGCACGTGTGCTTCAT+TGG - chr8.3:67651509-67651528 None:intergenic 50.0%
TGCAACGGCAACTTCAACTG+TGG + chr8.3:67652185-67652204 MS.gene84718:CDS 50.0%
! TGGGGTGAAGGCTATTTCCA+AGG + chr8.3:67651274-67651293 MS.gene84718:CDS 50.0%
ACCGGAGACGATACCGATGT+CGG - chr8.3:67651722-67651741 None:intergenic 55.0%
ATCGGTATCGTCTCCGGTTC+CGG + chr8.3:67651724-67651743 MS.gene84718:CDS 55.0%
TCTTCCGCGCTCATCTCGTT+AGG - chr8.3:67652420-67652439 None:intergenic 55.0%
GCCGACATCGGTATCGTCTC+CGG + chr8.3:67651718-67651737 MS.gene84718:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 67651094 67652482 67651094 ID=MS.gene84718
chr8.3 mRNA 67651094 67652482 67651094 ID=MS.gene84718.t1;Parent=MS.gene84718
chr8.3 exon 67651094 67652482 67651094 ID=MS.gene84718.t1.exon1;Parent=MS.gene84718.t1
chr8.3 CDS 67651094 67652482 67651094 ID=cds.MS.gene84718.t1;Parent=MS.gene84718.t1
Gene Sequence

>MS.gene84718

ATGTCACCTTCTTCATCTTCCTCTATGATATCATTTCCTCATGAAAATGAAAACCCTTCACCACAAACCCTAACCCTTCAACAAAAACTTCAATTATTAGTCCAAACCCAATCCGAAAATTGGGTCTATGCCATTTTGTGGCAAACAACAAAAGATGAAAAAGGTAAACCTTTCTTATCTTGGGGTGAAGGCTATTTCCAAGGAACCAAAGAAACAGCAATTTCAAACAAACAACACGACAGCAACACGTGTACAAATGGTGATGCTGAATGGTTCTATGTGATGTCGTTGAGTCGAAATTTTTCCATAGGTAATGCTTCTTCAATTTCATTACCTGGTAAAGCTTTTGCTTTAGATTCTGTACTTTGGTTGAATAGTAAACATGAACTTCAAATCTATAACTGTGAGAGATCCAATGAAGCACACGTGCATGGAATTGAAACATTGATTTGTATTCCGACAACTAACGGTGTAATCGAAATGGGTTCTTATGACAATATCCAACAAAATTGGAACCTAGTACACCAAGCCAAATCTATGTTCCAAACATCCACATCAGAATCTAACTCAGAAATAGATCTTATCTCCACAAACCCACTTGACAAATCTATGTTCCAAACATTCGCCGACATCGGTATCGTCTCCGGTTCCGGAGAAGACACTGATGAGACACAAAAAACAATGAACCAAAATTTGCAACAAATGAATAACATTGTTTCATCATGTTCTTTTGATTCTGAAAATTCTGATTCAGAATATTGTCCACCATTAGCAACACCAACAAGAATTGAAAAAGATTCTTTTGAGAAAAGAGAACCTAAGAAGAGAGGAAGAAAACCGTTATCAGGAACTCAAACACCGTTAAACCATGTGGAAGCAGAGAGACAAAGGAGAGAGAAACTAAACCATAGATTCTATGCTTTAAGATCTGTGGTTCCAAATGTTTCAAAGATGGATAAAGCTTCTCTTTTATCAGATGCAGTGGATTACATCAACGAGTTAAAAGCAAAGATTGAAGATTTAGAATCAGGGCAACAAAAGGAATCGAAAAAAGTGAAGATGGAAATGATGGAAATTGTCGATAATAACAGTGCAACGGCAACTTCAACTGTGGTAGACCAAAAAAGACCTTGTAATGTTAATGCTTTTGAGATTGATGTGAAGATAATAGGAAATGATGCTATGGTGAGAGTTCAAAGTGAGAATGTTAATCACCCTAGTGCAAGATTAATGAGTGTATTGAAGGATTTGGAGTTTCAGGTACATCATGCTAGTATATCGACTGTTAATGAAGTTATGGTTCAAGATGTTGTTGTTCGAGTTCCTAACGAGATGAGCGCGGAAGAGAGTCTAATTAGATCAGCCATTCTCATGAAATTAGGTCAGTAG
Protein sequence

>MS.gene84718.t1

MSPSSSSSMISFPHENENPSPQTLTLQQKLQLLVQTQSENWVYAILWQTTKDEKGKPFLSWGEGYFQGTKETAISNKQHDSNTCTNGDAEWFYVMSLSRNFSIGNASSISLPGKAFALDSVLWLNSKHELQIYNCERSNEAHVHGIETLICIPTTNGVIEMGSYDNIQQNWNLVHQAKSMFQTSTSESNSEIDLISTNPLDKSMFQTFADIGIVSGSGEDTDETQKTMNQNLQQMNNIVSSCSFDSENSDSEYCPPLATPTRIEKDSFEKREPKKRGRKPLSGTQTPLNHVEAERQRREKLNHRFYALRSVVPNVSKMDKASLLSDAVDYINELKAKIEDLESGQQKESKKVKMEMMEIVDNNSATATSTVVDQKRPCNVNAFEIDVKIIGNDAMVRVQSENVNHPSARLMSVLKDLEFQVHHASISTVNEVMVQDVVVRVPNEMSAEESLIRSAILMKLGQ